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  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (312)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 37-43 
    ISSN: 1573-0867
    Keywords: alfalfa ; gneiss ; Italian ryegrass ; maize ; mineral K ; pak-choi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The ability of plant types to release mineral K from little weathered gneiss for a mixture of particle size fractions of less than 10 mm, as well as for two separated size fractions (2 mm〈D〈5 mm, and 1 mm 〈D〈2 mm) were compared in pot experiments with maize (Zea mays L. cv. ND60), pak-choi (Brassica campestrisL. ssp.chinensis (L.) Mokina. var. cammunis Tsen et Lee, cv. Wuyueman) and two alfalfa cultivars (Medicago sativa L. cv. Asta and Haifei). Release of mineral K was significantly stimulated by maize, pak-choi and ryegrass, implying a direct mobilization of mineral K by plant roots. The net release of mineral K was greatly influenced by plant species. Among these, the more profound release of mineral K was observed with maize and ryegrass. Besides, the mobilization of mineral K was negatively correlated with the particle size of gneiss. The difference in net release of K from gneiss between two size fractions decreased in the order: maize 〉 ryegrass 〉 pak-choi.
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  • 2
    Electronic Resource
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    Springer
    Nutrient cycling in agroecosystems 57 (2000), S. 195-206 
    ISSN: 1573-0867
    Keywords: crop residue ; decomposition ; maize ; nutrient dynamics ; soybean ; sunflower
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The decomposition rates of different plant parts of maize (Zea mays L.; Gramineae), soybean [Glycine max (L.) Merr.; Leguminosae] and sunflower (Helianthus annuus L.; Compositae) were studied in soils with different physicochemical characteristics, and their contribution to nutrient availability was assessed. Litter decomposition rates were affected by plant species, plant part, and soil characteristics. In site A (SiCL soil), loss of litter mass was highest in soybean followed by sunflower and maize. In site B (Loam soil), loss of litter mass for soybean and sunflower was almost the same, while for maize it was lower. Nutrient release was high when their soil concentration was initially low. The higher the initial concentration of a nutrient in a plant part the greater its release rate. Nutrients, especially N, released from maize litter mass will be available to successive crops for a longer period than for soybean and sunflower, and are unaffected by soil texture. Nutrients are easily removed from sunflower and soybeans and are more likely to be lost through leaching than nutrients from maize.
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  • 3
    ISSN: 1572-9788
    Keywords: AFLP® ; genetic diversity ; methylation AFLP® ; polymorphism information content ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract AFLP® markers generated by CNG methylation sensitive (PstI/MseI) and CNG methylation insensitive (EcoRI/MseI) enzyme combinations and AFLP markers collected from hypomethylated (PstI/MseI) and hypermethylated (m PstI/MseI) regions were compared for their polymorphism information content, sampling variance and patterns of genetic diversity in a representative sample of 33 inbred lines of maize (Zea mays L.). We demonstrate that the mean polymorphism information content generated by sets of PstI/MseI and m PstI/MseI markers (0.38) is significantly higher than by sets ofEcoRI/MseI markers (0.33). Also the sampling variance highlighted the distinctive nature of the (m) PstI/MseI markers: to achieve a mean standard deviation of 5% in the estimation of genetic distance among the 33 inbreds, the PstI/MseI and m PstI/MseI marker sets (135 and 129 markers, respectively) are clearly smaller than the EcoRI/MseI marker set (173 markers). A further minimizing of the sampling variance of AFLP data in the estimation of genetic similarities was obtained by reducing marker information redundancy by selecting markers evenly distributed over each chromosome: a set of only 106 AFLP markers, sampled conditionally on their genetic map position, was required for a mean standard deviation of 5% in the estimation of genetic distance among the 33 inbreds.
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  • 4
    ISSN: 1573-5036
    Keywords: agroforestry ; Grevillea robusta ; maize ; root competition ; root length ; Senna spectabilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Complementarity in the distribution of tree and crop root systems is important to minimise competition for resources whilst maximising resource use in agroforestry systems. A field study was conducted on a kaolinitic Oxisol in the sub-humid highlands of western Kenya to compare the distribution and dynamics of root length and biomass of a 3-year-old Grevillea robusta A. Cunn. ex R. Br. (grevillea) tree row and a 3-year-old Senna spectabilis DC. (senna) hedgerow grown with Zea mays L. (maize). Tree roots were sampled to a 300 cm depth and 525 cm distance from the tree rows, both before and after maize cropping. Maize roots were sampled at two distances from the tree rows (75–150 cm and 450–525 cm) to a maximum depth of 180 cm, at three developmental stages. The mean root length density (Lrv) of the trees in the upper 15 cm was 0.55 cm cm−3 for grevillea and 1.44 cm cm−3 for senna, at the start of the cropping season. The Lrv of senna decreased at every depth during the cropping season, whereas the Lrv of grevillea only decreased in the crop rooting zone. The fine root length of the trees decreased by about 35% for grevillea and 65% for senna, because of maize competition, manual weeding, seasonal senescence or pruning regime (senna). At anthesis, the Lrv of maize in the upper 15 cm was between 0.8 and 1.5 cm cm−3. Maize root length decreased with greater proximity to the tree rows, potentially reducing its ability to compete for soil resources. However, the specific root length (m g−1) of maize was about twice that of the trees, so may have had a competitive uptake advantage even when tree root length was greater. Differences in maize fine root length and biomass suggest that competition for soil resources and hence fine root length may have been more important for maize grown with senna than grevillea.
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  • 5
    ISSN: 1573-5060
    Keywords: durable resistance ; hybrids ; maize ; marker-assisted selection ; pearl millet ; resistance gene deployment strategies ; rice ; sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Marker-assisted selection (MAS) for resistance genes (R-genes), identified using molecular markers and quantitative trait loci (QTL) analysis, is now possible in many crops. MAS can be used to pyramid several R-genes into a single host genotype. However, this may not provide durable genetic resistance because the pathogen is exposed to a full homozygous pyramid during hybrid seed production and to a full heterozygous pyramid in the resultant hybrid. Alternative gene deployment strategies that generate genetic variability were analysed, for hybrid cereal cultivars of pearl millet, maize, sorghum and rice, using maintainer lines (B-lines) with two smaller complementary pyramids. An F1 seed parent, produced on two such B-lines, can be used to produce a three-way hybrid. All target loci are heterozygous for resistance alleles in the F1 seed parent, and the pathogen is exposed in the hybrid to a host population that is heterogeneous and heterozygous for alleles at the resistance loci targeted by MAS. Alternatively, single-cross hybrids can be made on seed parents that are maintained by two B-lines that differ for the complementary resistance gene pyramids. In a cross-pollinated crop, the B-lines are allowed to intermate to produce a synthetic B-line. In an inbreeding crop, the B-lines are equivalent to a two-component multiline variety. In inbreeding crops, because there is no intermating between the B-line components, the resultant synthetic seed parents have a higher frequency of genotypes with resistance alleles (R-alleles) at several resistance loci. However, in both cross-pollinated and inbreeding crops the genotypic structure in the hybrids is almost the same. All alternatives to a single-cross hybrid having a full pyramid produce hybrid cultivars having lower frequencies of resistance alleles. The frequency of genotypes having R-alleles at several loci increases greatly in both seed parent and hybrid when the overall frequency of R-alleles in the maintainer lines increases. This is simply done by adding a maintainer line that has a full pyramid or by the component lines having overlapping pyramids.
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  • 6
    ISSN: 1573-5060
    Keywords: collaborative/participatory plant breeding ; farmer seed selection ; maize ; Mexico ; response to selection ; selection differential ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Formal plant breeders could contribute much to collaboration with farmers for improving crop varieties for local use. To do so outside researchers must have some understanding of local selection practices and their impact on crop populations in terms of the genetic theory underlying plant breeding. In this research we integrated methods from social and biological sciences to better understand selection and its consequences from farmers' perspectives but based on the concepts used by plant breeders. Among the households we worked with, farmers' selection practices were not always effective yet they understood the reasons for this and had no expectations for response to selection in some traits given the methods available to them. Farmers' statements, practices and genetic perceptions regarding selection and the genetic response of their maize populations to their selection indicate selection objectives different than may be typically assumed, suggesting a role for plant breeder collaboration with farmers.
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  • 7
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    Plant growth regulation 32 (2000), S. 239-243 
    ISSN: 1573-5087
    Keywords: IAA metabolism ; IAA-aspartate ; IAA-glucose ; light ; maize ; plant growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The inhibitory effect of light on the growth of plantscorrelates with a decrease of free IAA content in their tissues andmight be mediated through changes of IAA metabolism. In different partsof Zea mays L. seedlings (roots, mesocotyls and coleoptiles)that respond to light with a different growth rate, the effect of lighton the formation of IAA metabolites was examined in feeding experimentswith 14C-IAA. In all tissues, IAA was taken up andmetabolised mainly into six compounds, four of them were tentativelyidentified as IAA-1-O-glucose (IAGlc), IAA-myo-inositol, indoleacetamide and IAA-aspartate (IAAsp). IAA was metabolised most slowly inthe roots. In coleoptiles and mesocotyls, IAGlc was the most abundantmetabolite, except for mesocotyls in the light. In roots, a relativelylarge amount of IAA was also metabolised into IAAsp. Light stimulatedthe rate of IAA metabolism in all tissues, but its effect on theconversion of IAA was exceptionally high in mesocotyls. In mesocotyltissue the conversion into IAAsp was greatly stimulated by light.Conversely, the content of IAGlc in mesocotyls was decreased by light.Since light inhibited mesocotyl growth significantly and specifically,it is possible that the high capacity of mesocotyls to synthesise IAAspin the light may have caused a depletion of free IAA, which then led toan inhibition of growth. In mesocotyls from the light-grown plants IAAconjugated into IAGlc was probably used for IAAspbiosynthesis.
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  • 8
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    Plant growth regulation 30 (2000), S. 23-29 
    ISSN: 1573-5087
    Keywords: coleoptiles ; IAA metabolism ; IAA-aspartate ; IAA-glucose ; light ; maize ; mesocotyls
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Carbon 14-labelled indole-3-acetic acid (IAA) was fed to segments of shoots of Zea mays seedlings grown in light or dark to find the effect of light on IAA metabolism. The seedling parts coleoptile, with enclosed leaf, and mesocotyl were also used to examine differences in IAA metabolism between tissue types. The rate of metabolite formation as a function of time ranging from 1 to 12 hours was determined. Light did not significantly influence the amount of IAA taken up, but significantly increased its rate of metabolism and greatly increased the content of amide conjugates formed. There were also differences in metabolism depending on tissue type. In all tissues, IAA was metabolized mainly into six compounds. Four were tentatively identified as IAA-glucose (IAGlc), IAA-myo-inositol} (IAInos), indole acetamide (IAAm) and IAA-aspartic acid (IAAsp). 1-O-IAA-D-glucose (1-O-IAGlc) was the first conjugate formed and, except for mesocotyls in the light, it was the most abundant conjugate in maize tissue. In mesocotyl tissue the conversion of IAA into IAAsp was greatly stimulated by light, and the biosynthesis of IAAsp exceeded that of IAGlc. Since light strongly inhibited the growth of the mesocotyl, it is possible that the stimulation of IAAsp synthesis by light causes depletion of free IAA with resultant inhibition of mesocotyl growth.
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  • 9
    ISSN: 1573-5036
    Keywords: Fe nutrition ; intercropping ; maize ; peanut ; rhizosphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Both rhizobox and field experiments were conducted to investigate nutritional interactions between peanut and maize in intercropping systems for Fe acquistion. Field observations indicated that Fe deficiency chlorosis symptoms in peanut grown in monoculture were more severe and widespread compared to those of peanuts intercropped with maize. This indicated a marked improvement in the iron nutrition of peanut intercropped with maize in the field and was further studied. In experiments with rhizoboxes, roots of maize and peanut were either allowed to interact with each other or prevented from making contact by inserting a solid plate between the root systems of the two species. A field experiment for four cropping treatments were examined: peanut grown separately in monoculture, normal peanut/maize intercropping, peanut/maize intercropping with solid plates between the root systems of the two crop species and peanut/maize intercropping with 30 μm nylon nets between the root systems. The results show that the chlorophyll and HCl-extractable Fe concentrations in young leaves of peanut in the intercropping system with unrestricted interactions of the roots of both plant species were much higher than those of peanut in monoculture. In the nylon mesh treatment, the beneficial effects of the maize extended to row 3. The improvement of Fe nutrition in the intercropping system got reduced but not diminished completely in the treatment with nylon net. It is suggested that the improvement in the Fe nutrition of peanut intercropped with maize was mainly caused by rhizosphere interactions between peanut and maize.
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  • 10
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    Nutrient cycling in agroecosystems 55 (1999), S. 175-185 
    ISSN: 1573-0867
    Keywords: fertilizer recommendation ; maize ; soil mineral nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fifteen field trials were conducted to evaluate soil mineral N measurement as a means for quantifying the total N supply to forage maize and so to form the basis for fertilizer recommendations on a crop-specific basis. In every trial, 4 rates of cattle manure N (nominally 0, 80, 160, 240 kg N per ha) and 4 rates of ammonium nitrate (0, 50, 100, 150 kg N per ha) were factorially combined. Soil mineral N measurements were made before manure application, at the time of maize drilling, 7-10 weeks after drilling and after harvest. Measurements on control treatments which received no manure or ammonium nitrate showed extensive net mineralisation of soil N (mean 140 kg N per ha) in the 7-10 weeks after drilling followed by a decrease due to crop uptake, and probably net immobilisation, of approximately the same amount by harvest. This net mineralisation was probably the reason why only one trial showed a significant dry-matter yield response to ammonium nitrate. Results indicated that , to be useful for N recommendations, soil mineral N measurements should be taken 7-10 weeks after drilling. Only if the amount of mineral N at this time is less than expected crop N offtake should fertilizer N be applied. A mean of around 64% of the N applied in ammonium nitrate could be accounted for in soil mineral N after harvest of the maize, although this was reduced to 24% in the single trial where a dry-matter response to ammonium nitrate was recorded.
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  • 11
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    Nutrient cycling in agroecosystems 55 (1999), S. 95-105 
    ISSN: 1573-0867
    Keywords: cover crops ; savanna ; N fertilizer ; maize ; cowpea ; N fertilizer replacement value
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Integrated soil management with leguminous cover crops was studied at two sites in the northern Guinea savanna zone of northern Nigeria, Kaduna (190 day growing season) and Bauchi (150 days). One-year planted fallows of mucuna, lablab, and crotalaria were compared with natural grass fallow and cowpea controls. All treatments were followed by a maize test crop in the second year with 0, 30, or 60 kg N ha−1 as urea. Above ground legume residues were not incorporated into the soil and most residues were burned early in the dry season at the Kaduna site. Legume rotation increased soil total N, maize growth in greenhouse pots, and dry matter and N accumulation of maize. Response of maize grain yield to 30 kg N ha−1 as urea was highly significant at both sites and much greater than the response to legume rotation. The mean N fertilizer replacement value from legume rotation was 14 kg N ha−1 at Kaduna and 6 kg N ha−1 at Bauchi. W ith no N applied to the maize test crop, maize grain yield following legume fallow was 365 kg ha−1 higher than natural fallow at Bauchi and 235 kg ha−1 higher at Kaduna. The benefit of specific legume fallows to subsequent maize was mostly related to above ground N of the previous legume at Bauchi, where residues were protected from fire and grazing. At Kaduna, where fallow vegetation was burned, maize yield was related to estimated below ground N. The results show that legume rotation alone results in small maize yield increases in the dry savanna zone.
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  • 12
    ISSN: 1573-8469
    Keywords: ear rot ; leaf blight ; mid-altitude ; maize ; Stenocarpella macrospora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Inheritance of resistance to Stenocarpella macrospora (Earle) Sutton (syn. Diplodia macrospora Earle) ear rot of maize was studied among selected maize populations in the mid-altitude (1280 m) agro-ecological zone of Nigeria. Diallel analysis among the populations showed significant values for general combining ability (GCA) and specific combining ability (SCA) effects at 5% and 1% levels respectively. Variance components of GCA and SCA on Stenocarpella ear rot were 0.019 and 0.627 respectively, indicating that non-additive genes play major roles in the inheritance of Stenocarpella ear rot resistance. The GCA and SCA effects were relatively dependent on the materials involved in the evaluations. Generation mean analysis was used on five selected parent inbreds (2 resistance and 3 susceptible crossed to give P1, P2, F1, BC1, BC2 and F2 generations). Estimates of the six parameters on ear rot indicate that dominance gene effects made the major contribution to variation in ear rot of maize in the crosses studied. The magnitude and significance of the estimates for digenic effects in the crosses suggest that epistatic gene effects are present and important in the basic mechanism of Stenocarpella ear rot inheritance in the populations studied. Additive effects have only minor importance in the total variation.
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  • 13
    ISSN: 1573-5036
    Keywords: maize ; matric water potential ; rhizosphere ; soil:root adhesion ; soil:root contact ; soil surface properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was designed to investigate the strength of attachment of plant seedling roots to the soil in which they were grown. The study also assessed the effects of differing soil textures and differing soil matric potentials upon the strength of the root:soil attachment. A device for growing roots upon a soil surface was designed, and was used to produce roots which were attached to the soil. In order to quantify root:soil adhesion, roots of maize seedlings, grown on the soil surface, were subsequently peeled off using a universal test machine, in conjunction with simultaneous time-lapse video observation. To clarify the partitioning of energy in the root:soil peeling test, separate mechanical tests on roots, and on two adherent remoulded topsoil balls were also carried out. The seedling root was characterised by a low bending stiffness. The energy stored in bending was negligible, compared to the root:soil adhesion energy. The mechanical properties of two adherent remoulded topsoil balls were a decrease of the soil:soil adhesion energy as the soil:soil plastic energy increased. These two parameters were therefore interdependent. Using a video-camera system, it was possible to separate the different processes occurring during the root:soil peeling test, in particular, the seed:soil adhesion and the root:soil soil adhesion. An interpretation of the complex and variable force:displacement curves was thus possible, enabling calculation of the root:soil interfacial rupture energy. At a given suction (10 kPa), the results of the peeling test showed a clear soil texture effect on the value of the root:soil interfacial rupture energy. In contrast, for the same silty topsoil, the effect of the soil water suction on the value of the interfacial rupture energy was very moderate. The root:soil interfacial rupture energy was controlled mainly by a product of microscopic soil specific surface area and the macroscopic contact surface area between the root and the soil. Biological and physical interactions contributing to root:soil adhesion such as root:soil interlocking mechanics were also analysed and discussed.
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  • 14
    ISSN: 1573-5036
    Keywords: calcite ; goethite ; maize ; phosphate ; H+ release ; Brassica napus L. ; Zea Mays L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In calcareous soils the dynamics of phosphorus is controlled by calcite and iron oxides such as goethite which strongly retain P and consequently maintain low P concentrations in soil solution. Plants can drastically change chemical conditions in the rhizosphere, in particular by releasing H+ or OH− or by excreting organic anions. By modifying the dissolution/precipitation and desorption/adsorption equilibria, roots can influence the mobility of soil P. The aim of this work was to test whether H+ or OH− release can induce the mobilization of P in the rhizosphere of maize and rape supplied with NO3-N or NH4-N and grown on synthetic phosphated calcite or goethite as sole source of P. With P-calcite, the mobilization of P was generally related to the acidification of the rhizosphere. With P-goethite, rhizosphere acidification induced some increase of DTPA-extractable Fe and hence dissolution of goethite. Rhizosphere P was concomitantly depleted but the mechanisms involved are less clear. The difference in behavior of the two species is discussed.
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  • 15
    ISSN: 1573-5036
    Keywords: agroforestry ; Gliricidia sepium ; inorganic nitrogen ; maize ; nitrogen mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Many soils of southern Africa are severely N deficient, but inorganic fertilizers are unaffordable for most subsistence farmers. Rotations and intercrops of legumes with crops may alleviate N deficiency through biological N2 fixation and redistribution of subsoil N to the surface. We monitored soil inorganic N dynamics for two seasons in a gliricidia [Gliricidia sepium (Jacq.) Walp.] – maize (Zea mays L.) intercrop in the unimodal rainfall area of southern Malawi. One maize crop per year was grown with or without interplanted gliricidia, in factorial combination with three rates of N (0, 24 or 48 kg N ha-1). Application of gliricidia prunings increased (p 〈 0.001) topsoil (0 to 20 cm) inorganic N at the end of the dry season and during the early rains. Differences between plus and minus gliricidia treatments were less when total inorganic N to 1-m depth was summed. A greater proportion of the total inorganic N to 1-m depth occurred in the topsoil (0 to 20 cm) when gliricidia was present, suggesting that redistribution of subsoil N to the surface accounted for part of the N increase by gliricidia. Gliricidia lowered (p 〈 0.05) subsoil water content during drier periods. Gliricidia plots accumulated more (p 〈 0.01) ammonium-N during the dry season. Nitrate-N remained constant during the dry season but rose rapidly in gliricidia plots after the onset of rains. A 2-factor model including preseason inorganic N and anaerobic N mineralization potential accounted for 84% of the variability in maize yields for the two seasons' data combined. The combination of preseason inorganic N and potential N mineralization appears to provide a good estimate of N supply to maize in systems receiving both organic and inorganic sources of N.
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  • 16
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    Euphytica 108 (1999), S. 145-150 
    ISSN: 1573-5060
    Keywords: maize ; North Carolina model 2 ; salinity tolerance ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic basis of salt tolerance was examined in selected salt tolerant and sensitive material from a sample of accessions previously assessed for variability in salinity tolerance. The North Carolina Model 2 Design and analysis was followed, tolerance being assessed in 10-day-old seedlings grown in salinized solution culture at control (0 mM), 60 mM and 80 mM NaCl concentrations). Salinity tolerance was shown to be under the control of genes with additive and non-additive effects, with broad and narrow sense heritability estimates being approximately 0.7 and 0.4 over all treatments.
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  • 17
    ISSN: 1573-0867
    Keywords: applied N and P ; crop residue ; maize ; Residual N and P ; Typic Pellustert ; Typic Ustorthent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field trials were conducted on two soil types for seven years (1988–1994) to investigate grain yield response of maize to crop residue application as influenced by varying rates of applied and residual N and P fertilizers. Yearly application of N and P fertilizers at both one-half and full recommended rates resulted in grain yield increases of more than 500 and 1100 kg ha-1, respectively over application of only crop residue. Moreover, grain yield responses due to residual N and P fertilizers applied only during the first year were found to be comparable to the yearly applications of these fertilizers. Rainfall and soil type have exerted considerable influences on the grain yield response obtained in this study. Grain yield exhibited a corresponding decrease with decreasing rainfall. Grain yield increases on Typic Pellustert were relatively higher than on Typic Ustorthent.
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  • 18
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    Agroforestry systems 47 (1999), S. 67-91 
    ISSN: 1572-9680
    Keywords: cover crops ; farmer innovation ; land tenure ; maize ; nutrient cycling ; velvetbean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In many parts of Central America long fallow periods are no longer feasible due to increasing land pressures. Farmers in northern Honduras have developed and diffused from farmer-to-farmer a maize cropping system using velvetbean (Mucuna spp.) as a short-term fallow. High total annual rainfall in a bimodal distribution is favorable to the system because it allows the completion of two rainfed cropping cycles annually. The first season is dedicated to the production of the mucuna crop and the second season is dedicated to maize. Soil pH and exchangeable Ca were not reduced during a 15-year period of continuous mucuna use. Soil organic matter, infiltration, and porosity increased with continuous mucuna use. Maize yields in fields with continuous rotation of mucuna were on average double those obtained without mucuna. The mucuna system was more profitable than the existing alternative bush-fallow system due to higher returns to land and labor resulting from higher yields, lower weeding and land preparation costs, and reduced risk of drought stress. The relative profitability of the mucuna system was also enhanced by seasonally high maize prices during the second season when maize is harvested in the mucuna system. Relatively easy access to land through inexpensive land ownership and land rental markets has made it possible for even small-scale farmers to dedicate land to the mucuna system. Land-use intensity is increasing in the region, however, as land is converted to pastures for cattle production. The opportunity costs of keeping land in the mucuna system, while also accessing land for first-season maize and other crops, are also increasing. These experiences remind us that a viable livelihood is the primary factor in farmers' decision making about adoption particular components of farming systems.
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  • 19
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    Agroforestry systems 40 (1998), S. 41-58 
    ISSN: 1572-9680
    Keywords: hedgerow ; maize ; mulch ; Pennisetum purpureum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Napier grass strips are gaining some acceptance in Southeast Asia as a vegetative means of soil erosion control, due to the relative ease of establishment and management. In addition, the napier grass strips aid productivity of agroforestry systems by providing mulch or by being fed to livestock. In this paper, a bioeconomic comparison of different ways of utilising the napier grass cuttings is undertaken. The three uses examined for the cuttings were: as mulch in the alley areas, sold as fodder, and fed to animals with manure applied back to the system. The analysis linked the soils-oriented SCUAF model with economic and animal component models. SCUAF was parameterized using field data based on farmer interviews and field visits. Farmer interviews and field visits were conducted at Claveria, Misamis Oriental to gather additional data regarding costs and returns associated with animal production, and also to obtain the farm gate price of napier. Predicted biophysical results produced by the SCUAF model showed that application of napier grass cuttings, back into the system, directly as mulch or indirectly as animal manure resulted in higher maize and napier yields, lower soil erosion rates, and less of a reduction in soil mineral nitrogen and soil labile carbon levels. The comparison was made with the case where napier was sold off-farm as fodder. Cost-benefit analysis showed that higher economic returns were gained when napier grass cuttings were fed to animals, rather than applied directly as mulch. This economic benefit was attributed to the draught and transport services rendered by the animal and to a change in the value of the animals. This analysis highlights the value of utilizing animals within a napier grass strip system for control of erosion.
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  • 20
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    Integrated pest management reviews 3 (1998), S. 177-188 
    ISSN: 1572-9745
    Keywords: Cercospora zeae-maydis ; control ; grey leaf spot ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Grey leaf spot of maize, (Zea maydis L.) (Cercospora zeae-maydis) Tehon and Daniels, is one of the most destructive leaf diseases of maize. The distribution and severity of grey leaf spot has increased over the past 10 to 15 years. The United States and South Africa are main areas where research on the disease has been concentrated. The research results have provided valuable and significant insight into pathogen epidemiology and allowed an integrated management system to be developed. However, management options are a result of the agricultural system under which maize is produced. The pathogen survives only on maize, so crop rotation and stubble management are major factors in disease management, but resistant hybrids offer the best option for economic control. Many other factors, such as soil fertility, plant density, irrigation and chemical control, can significantly influence a grey leaf spot epidemic, although a single management practice will not control the pathogen effectively.
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  • 21
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    European journal of plant pathology 104 (1998), S. 611-617 
    ISSN: 1573-8469
    Keywords: Exserohilum turcicum ; Johnson grass ; maize ; northern corn leaf blight ; population genetic structure ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Setosphaeria turcica is the causal agent of northern corn leaf blight, a foliar maize disease of worldwide economic importance. In Europe, its severity increases. To investigate the pathogen's population-genetic structure in central Europe, a total of 80 isolates was sampled in Germany, Switzerland, France, Austria, and Hungary and investigated with 52 random amplified polymorphic DNA (RAPD) markers. The mating type of the isolates was determined in testcrosses. Among the 73 isolates from maize there were 26 different RAPD haplotypes. All isolates with identical haplotype are considered clonemates. The haplotype shared by most members was represented by 22 isolates from Germany, Switzerland, and France, indicating high fitness and substantial migration. Only a single clone had members in both southeastern Austria and southwestern Switzerland, suggesting that the Alps constitute a major barrier for this pathogen. Several haplotypes differed by only one or two RAPD bands from the predominant haplotype and may have arisen by mutation. Few other clonal lineages were detected. The evolution of some haplotypes could not be explained by mutation alone. Sexual recombination may rarely occur. In population samples from Germany, Switzerland, and France, mating type MAT2 was predominating, while most isolates from Austria and Hungary had MAT1. Seven isolates from Johnson grass (Sorghum halepense), an alternative host of S. turcica, were clonemates and very different in RAPD haplotypes from all isolates collected from maize.
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  • 22
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    Plant and soil 198 (1998), S. 185-192 
    ISSN: 1573-5036
    Keywords: colonization ; crop-rotation ; maize ; mycorrhiza ; phosphorus ; tillage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We selected three crop production practices; crop rotation, tillage and phosphorus fertilization, all known to affect arbuscular mycorrhiza (AM) development, to study early AM intraradical colonization in maize. Half of the plots were planted during the first year with either a host (maize, Zea mays L.) or a non-host (canola, Brassica napus L.) crop, and all of them with maize for the second year. Tillage and P fertilization treatments were applied to the plots in the second year. Mycorrhiza development in maize was measured in pot culture bioassays conducted before planting and after harvest of the previous and the subsequent crops, and in the field during the second crop season. Previous cropping of a soil with canola (Brassica napus L.), a non-host plant species, delayed mycorrhiza development of maize in a bioassay conducted with that soil in comparison with a previous cropping cycle with maize (Zea mays L.) or with the original plant species in the field site, bromegrass (Bromus inermis Leys.) and alfalfa (Medicago sativa L.). The delay in mycorrhiza development after cropping with canola was also observed in samples taken from the field and in a bioassay, both conducted at the beginning of the subsequent cropping cycle. Tillage had, on average, little effect on intraradical colonization either in the field or in the bioassays. Phosphorus fertilization also had little effect on mycorrhiza development in the field. Crop rotation with a non-host had the strongest effect on intraradical mycorrhiza development of the three practices studied.
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  • 23
    ISSN: 1573-5036
    Keywords: intercrops ; maize ; model ; roots ; sorghum ; water transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A knowledge of plant interactions above and below ground with respect to water is essential to understand the performance of intercrop systems. In this study, a physically based framework is proposed to analyse the competition for soil water in the case of intercropped plants. A radiative transfer model, associated with a transpiration-partitioning model based on a modified form of the Penman-Monteith equation, was used to estimate the evaporative demand of maize (Zea mays L.) and sorghum ( Sorghum vulgare R.) intercrops. In order to model soil–root water transport, the root water potential of each species was calculated so as to minimise the difference between the evaporative demand and the amount of water taken up by each species. A characterisation of the micrometeorological conditions (net radiation, photosynthetically active radiation, air temperature and humidity, rain), plant water relations (leaf area index, leaf water potential, stomatal conductance, sap flow measurements), as well as the two-component root systems and water balance (soil–root impacts, soil evaporation) was carried out during a 7-day experiment with densities of about 4.2 plant m-2 for both maize and sorghum. Comparison of the measured and calculated transpiration values shows that the slopes of the measured versus predicted regression lines for hourly transpiration were 0.823 and 0.778 for maize and sorghum, respectively. Overall trends in the variation of volumetric water content profiles are also reasonably well described. This model could be useful for analysing competition where several root systems are present under various environmental conditions.
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  • 24
    ISSN: 1573-5036
    Keywords: available soil water ; extraction pattern ; maize ; sorghum ; soya bean ; sunflower ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The estimation of soil water reserves is essential for irrigation management. The usual way of calculating these reserves, held between the soil moisture content at field capacity and the classical limit of −1.5 MPa considered as the lower limit of available water, over the rooting depth of the crop, does not correspond with the real behaviour of crops as regards their ability to extract soil water and should be only considered as the apparent available water (AAW). Measurements of moisture profiles made using a neutron probe soil moisture meter from 1970 until 1991 on unirrigated crops at the INRA Agronomy Station at Toulouse-Auzeville, France, on a deep silty clay soil with a high water holding capacity have enabled us to define the water extraction capacities of maize ( Zea mays L.), sunflower (Helianthus annuus L.), sorghum (Sorghum bicolor L. Moench), soya bean (Glycine max L. Merr.), and winter wheat (Triticum aestivum L.). The results show, not only that all the crops can extract soil water from beyond −1.5 MPa in the surface layers to varying degrees and depths, depending on the crop, but also that deeper down, AAW is not fully used, as the moisture profile gradually returns to field capacity. Of the five crops studied, maize extracts the most water from the top 0.5 m, removing 150% of AAW. This amount falls rapidly lower down, reaching nil at 1.6 m. Conversely sunflower extracts less near the surface, but uses all AAW up to 1.2 m, and still extracts 85% of AAW at 1.6 m. Sorghum is somewhat comparable to sunflower, but with a lower use over the entire profile. Soya bean exhibits strong extraction to 1.0 m, and then much less at depth. As to wheat, its extraction capability is quite high near the surface, and then falls steadily with depth where it is still 30% of AAW at 1.6 m. Soil moisture measurements realised on a bare soil during several successive years were used to fix the maximum soil evaporation and to suggest the contribution of crops in soil water depletion from uppermost layers. The water extraction capacities have been modelled and introduced into the model EPICphase, a modified version of the model EPIC, adapted for irrigation management. Four parameters have been introduced to simulate: (1) the rooting pattern of the crop (parameter α), (2) the degree of involvement of deep layers (parameter p), (3) the fraction of AAW beyond which crop transpiration is affected (parameter t) and (4) the intensity of extraction beyond the limit of −1.5 MPa as a function of soil depth (parameter d). Calibrated on the basis of the driest year since 1970 for each crop, the model was then validated under unirrigated conditions, and then tested on irrigated maize plots. Under unirrigated conditions, the simulations correctly reproduced the water extraction by the five crops, both in an extremely dry year and in a wet year. The observed differences between simulations and observations were found mostly at about 0.1 m depth, and were due to lack of precision of moisture measurements with the neutron probe. From 0.2 to 0.6 m the simulations have a tendency to overestimate the extraction. These differences are explained by water fluxes which are especially high in these layers because of the processes of evaporation from the soil and plant transpiration, which are difficult to simulate with precision. Below 0.6 m, a more stable zone where water movements are of minor importance, the simulations are very precise. For irrigated maize, the results show a very good fit between simulation and measurement, indicating that these water extraction capacity figures could be used for irrigation management provided that the rules for exploitation of the water reserves are well established.
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  • 25
    ISSN: 1573-5036
    Keywords: Grevillea robusta ; maize ; root length ; root biomass ; root sampling ; sieves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The purpose of this study was to investigate the effects of different mesh sizes on the recovery of root length and biomass and to determine whether the degree of recovery was influenced by plant species and sample location. Sieves of 2.0, 1.0, 0.5 and 0.25 mm (4.0, 1.0, 0.25 and 0.06 mm2) mesh sizes were used to recover and measure the root length and biomass of Zea mays L. (maize) at 0–15 cm and 30–45 cm depths and of Grevillea robusta A. Cunn. ex R. Br. (grevillea) at the same depths 1.0 m and 4.5 m from a line of grevillea trees. At 0–15 cm, the coarser sieves (sum collected with 2.0 and 1.0 mm sieves) recovered approximately 80% of the total root biomass measured, but only 60% of the root length. The proportion of total maize root length and biomass recovered by the coarser sieves decreased with soil depth. The proportion of total grevillea root length recovered by the coarser sieves was similar at the two soil depths, but increased slightly with distance from the tree line. The ≥ 0.5 mm sieves recovered between 93 and 96% of grevillea and maize root biomass and between 73 and 98% of their root length, depending on the sample location. Roots passing through the 0.5 mm sieve, but recovered by the 0.25 mm sieve were about 20% of total maize root length and grevillea root length at 1.0 m from the tree line but 〈 5% of the total grevillea root length at 4.5 m from the tree. Roots passing through the 0.5 mm sieve but recovered by the 0.25 mm sieve contributed only slightly to root biomass. Although the ≥ 0.5 mm sieves provided adequate measurements of root biomass, the ≥ 0.25 mm sieves were required for accurate measurement of fine root length. There was no universal correction for root length and biomass underestimation when large sieve sizes were used because the proportions of length and biomass recovered depended on the plant species and on soil depth and distance from the plant.
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  • 26
    ISSN: 1573-5036
    Keywords: acetylene inhibition ; denitrification ; irrigated field ; maize ; 15N balance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Denitrification and total N losses were quantified from an irrigated field cropped to maize and wheat, each receiving urea at 100 kg N ha-1. During the maize growing season (60 days), the denitrification loss measured directly by acetylene inhibition-soil cover method amounted 2.72 kg N ha-1 whereas total N loss measured by 15N balance was 39 kg ha-1. Most (87%) of the denitrification loss under maize occurred during the first two irrigation cycles. During the wheat growing season (150 days), the denitrification loss directly measured by acetylene inhibition-soil cover and acetylene inhibition-soil core methods was 1.14 and 3.39 kg N ha-1, respectively in contrast to 33 kg N ha-1 loss measured by 15N balance. Most (70-88%) of the denitrification loss under wheat occurred during the first three irrigation cycles. Soil moisture and NO 3 - -N were the major factors limiting denitrification under both crops. Higher N losses measured by 15N balance than C2H2 inhibition method were perhaps due to underestimation of denitrification by C2H2 inhibition method and losses other than denitrification, most probably NH3 volatilization.
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  • 27
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    Euphytica 104 (1998), S. 17-23 
    ISSN: 1573-5060
    Keywords: crossability ; D genome ; durum wheat ; embryo culture ; haploid ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract With the aim of examining crossability of durum wheat with maize, two sets of durum wheat genotypes and a set of D-genome chromosome substitution lines of the durum wheat variety ‘Langdon’ were crossed with maize, and followed by 2,4-dichlorophenoxyacetic acid (2,4-D) treatment in detached-tiller culture. In crosses of 25 durum wheat genotypes (breeding lines) with maize, percent frequencies of embryo formation increased from 1.4% to 2.8% by adding silver nitrate to the detached-tiller culture solution. In crosses of 32 durum wheat genotypes (advanced lines and varieties) with maize using the silver nitrate addition, frequencies of embryo formation ranged from 0.0% to 15.8%; seven genotypes showing more than 6.0% embryo formation frequency were related in their pedigrees. In crosses of a set of chromosome substitution lines with maize, higher frequencies of embryo formation were obtained in substitution lines with chromosomes 1D, 3D, 4D and 7D. These results suggest that 1) adding silver nitrate to the 2,4-D treatment increases overall frequency of embryo formation but is not effective enough to induce the development of seeds and embryos from all durum wheat genotypes, and 2) some D-genome chromosomes substituted in a durum wheat genetic background may enhance crossability with maize in combination with homoeologous chromosomes of durum wheat.
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  • 28
    ISSN: 1572-9680
    Keywords: alley-cropping ; credit ; erosion ; labour ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Considerable resources have been expended promoting hedgerow intercropping with shrub legumes to farmers in the Philippine uplands. Despite the resources committed to research and extension, persistent adoption by farmers has been limited to low cost versions of the technology including natural vegetation and grass strips. In this paper, cost-benefit analysis is used to compare the economic returns from traditional open-field maize farming with returns from intercropping maize between leguminous shrub hedgerows, natural vegetation strips and grass strips. An erosion/productivity model, Soil Changes Under Agroforestry, was used to predict the effect of erosion on maize yields. Key informant surveys with experienced maize farmers were used to derive production budgets for the alternative farming methods. The economic incentives revealed by the cost-benefit analysis help to explain the adoption of maize farming methods in the Philippine uplands. Open-field farming without hedgerows has been by far the most popular method of maize production, often with two or more fields cropped in rotation. There is little persistent adoption of hedgerow intercropping with shrub legumes because sustained maize yields are not realised rapidly enough to compensate farmers for establishment and maintenance costs. Natural vegetation and grass strips are more attractive to farmers because of lower establishment costs, and provide intermediate steps to adoption. Rural finance, commodity pricing and agrarian reform policies influence the incentives for maize farmers in the Philippine uplands to adopt and maintain hedgerow intercropping.
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  • 29
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    Agroforestry systems 41 (1998), S. 167-179 
    ISSN: 1572-9680
    Keywords: maize ; sample size ; sampling strategy ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Data from a Brazilian Inga/maize/bean alleycrop experiment are used to illustrate patterns of variation between and within crop rows and their possible effects in the determination of a sampling strategy. Three methods for the determination of sample sizes in agroforestry experiments are compared: the combined plant technique, the formula of Snedecor and Cochran and variance component estimation. Results show that variability in the edge rows was generally greater than variability in the inner rows and suggest three sampling strata – one for each of the two edge rows and the third for the inner rows. For all three sampling methods a sample size greater than ten plants per stratum per block gave little extra precision. Increasing the number of blocks improves precision and may permit a smaller sample size per row to achieve the same precision. The comparison of different methods of sample size determination shows that variance component estimation is the most flexible and efficient approach.
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  • 30
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    Plant and soil 199 (1998), S. 283-291 
    ISSN: 1573-5036
    Keywords: dry matter partitioning ; maize ; 15N ; nitrogen uptake ; nitrogen use efficiency ; senescence ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In eastern Canada, the use of fertilizer N has been identified as the most energy-consuming component of maize (Zea mays L.) grain production. As the economic and environmental costs of excessive N fertilization rise, there is an increased emphasis on selection of hybrids with greater N use efficiency (NUE; defined as the ratio of the amount of 15N recovered in grain or stover dry matter to the amount of fertilizer 15N applied to the soil in this study). Using an 15N-labelling approach, a field study was conducted on a tile-drained Brandon loam soil (Typic Endoaquoll) on the Central Experimental Farm at Ottawa, Canada (45°22′ N, 75°43′ W) in 1993 and 1994. Fertilizer N uptake and partitioning within the plant in relation to dry matter changes were monitored during development of a current stay-green maize hybrid and an older early-senescing hybrid grown with three fertilizer N levels (0, 100, 200 kg N ha-1). Dry matter, N concentration and15 N atom% enrichment of plant components were determined at five growth stages. The current stay-green hybrid, ‘Pioneer 3902’ had greater NUE than the old early-senescing hybrid, ‘Pride 5’, which was associated with 24% more dry matter production and 20% more N uptake during grain fill for Pioneer 3902. There was no indication of greater allocation of N to the grain in Pioneer 3902. Our data suggest that prolonged maintenance of green leaf area for photosynthate production during grain fill and the ability to take up available soil N later in grain filling are characteristics of maize hybrids with greater NUE.
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  • 31
    ISSN: 1573-5036
    Keywords: carbon-13 ; maize ; modelling ; physical protection ; stablecarbon isotope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cultivation of forest and grassland soils induces heavy changes in soil organic matter (SOM) dynamics. To better predict the effect of cultivation, there is a need to describe which organic pools are affected and to which extent. We used a chronosequence of thick humic forest soils converted to maize cultivation for 40 yr in southwest France. The dynamics of soil carbon was investigated through particle-size fractionation and the use of 13C allowed to distinguish forest-derived organic matter and new crop-derived organic matter. This partitioning of soil carbon by size on one hand and by age on the other provided a precise description of carbon turnover. The level towards which tend the organic pools under cultivation showed that the decay rates of soil carbon were one order of magnitude higher under cultivation than under forest. SOM can thus be considered as deprotected under cultivation. All size fractions appeared to be deprotected to the same extent. A progressive transfer of silt-sized C to clay-sized C was nevertheless suspected and attributed to the decreasing stability of fine silt-sized microaggregates with cultivation. SOM furthermore contained some very stable C present as silt-sized and possibly clay-sized particles. The turnover times of maize-derived organic matter was the same as that observed in similar soils cultivated for centuries. This indicated that the new conditions induced by cultivation were reached in the very first years after forest clearing and that the high initial SOM content and high mineralization rate of initial organic matter did not affect the dynamics of newly incorporated carbon.
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  • 32
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    Plant and soil 199 (1998), S. 177-186 
    ISSN: 1573-5036
    Keywords: colonization ; crop rotation ; harvest index ; maize ; mycorrhizae ; phosphorus ; tillage ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We conducted a field experiment to test the hypothesis that improved phosphorus nutrition occurs in maize plants with rapid arbuscular (AM) mycorrhizae development at early developmental stages and that this also is reflected in dry matter allocation and final yield. A split-split plot design was used with previous crop (Zea mays L.-maize and Brassica napus L.-canola), tillage practices (no-tillage or conventional tillage) and P fertilization (5 levels) as factors chosen to modify mycorrhizae development at early developmental stages of maize. Previous cropping with canola resulted in decreased shoot-P concentration and shoot growth of maize at early stages. No-tillage resulted in higher shoot-P concentration but lower shoot weight than conventional tillage. Greater shoot-P uptake was related to a rapid intraradical development of mycorrhizae (previous crop of maize) or rapid connection to a mycorrhizal mycelium network (no-tillage treatments). Maize yield and harvest index were lower after cropping with canola. The yield for conventional tillage was higher than that for no-tillage but the harvest index was lower. The hypothesis was supported at early stages of maize growth by the effect of previous crop but not by results of tillage, because an unknown factor reduced growth in the no-tillage system. The hypothesis was supported at maturity by increased biomass allocation to grain relative to total shoot weight in treatments with greater shoot-P concentration at early stages.
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  • 33
    ISSN: 1573-5036
    Keywords: image processing ; maize ; plant residue ; root diameter ; root length ; root washing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Historically, destructive root sampling has been labor intensive and requires manual separation of extraneous organic debris recovered along with the hydropneumatic elutriation method of separating plant roots from soils. Quantification of root system demographics by public domain National Institute of Health (NIH-Image) and Root Image Processing Laboratory (RIPL) image processing algorithms has eliminated much of the labor-intensive manual separation. This was accomplished by determining the best length to diameter ratio for each object during image analyses. Objects with a length to diameter ratio less than a given threshold are considered non-root materials and are rejected automatically by computer algorithms. Iterative analyses of length to diameter ratios showed that a 15:1 ratio was best for separating images of maize (Zea mays L.) roots from associated organic debris. Using this threshold ratio for a set of 24 soil cores, a highly significant correlation (r2 = 0.89) was obtained between computer image processed total root length per core and actual root length. A linear relationship (r2 = 0.80) was observed between root lengths determined by NIH-Image analyses and lengths determined independently by the RIPL imaging system, using the same maize root + debris samples. This correlation demonstrates that computer image processing provides opportunities for comparing root length parameters between different laboratories for samples containing debris.
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  • 34
    ISSN: 1573-5036
    Keywords: maize ; model ; root architecture ; root intersection ; root length ; stochastic geometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The spatial distribution of root length density (RLD) is important because it affects water and nutrient uptake. It is difficult to obtain reliable estimates of RLD because root systems are very variable and heterogeneous. We identified systematic trends, clustering, and anisotropy as geometrical properties of root systems, and studied their consequences for the sampling and observation of roots. We determined the degree of clustering by comparing the coefficient of variation of a simulated root system with that of a Boolean model. We also present an alternative theoretical derivation of the relation between RLD and root intersection density (RID) based on the theory of random processes of fibres. We show how systematic trends, clustering and anisotropy affect the theoretical relation between RLD and RID, and the consequences this has for measurement of RID in the field. We simulated the root systems of one hundred maize crops grown for a thermal time of 600 K d, and analysed the distribution of RLD and root intersection density RID on regular grids of locations throughout the simulated root systems. Systematic trends were most important in the surface layers, decreasing with depth. Clustering and anisotropy both increased with depth. Roots at depth had a bimodal distribution of root orientation, causing changes in the ratio of RLD/RID. The close proximity of the emerging lateral roots and the parent axis caused clustering which increased the coefficient of variation.
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  • 35
    ISSN: 1573-5060
    Keywords: interspecific cross ; haploid ; wheat ; maize ; rye chromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genotypic influence of both male and female parents on haploid production through interspecific crosses was studied using eight wheat and four maize genotypes. The average numbers of embryos and green haploid plantlets obtained per pollinated floret were 17.6% and 10.1%, respectively. Clear genotypic influence of the wheat genotype was detected, but heterozygosity of the wheat did not affect haploid production. Analogous response to anther culture and interspecific crossing was observed, still a wheat variety which did not respond to anther culture, produced 1.1 plantlets per pollinated spike upon maize pollination. This appears to be a major advantage of interspecific crossing compared to anther culture technique in wheat. Circumstantial evidence is presented for specific wheat × maize interaction on haploid plantlet formation. Rye chromatin enhanced haploid production but only in a complete 1B/1R substitution line. Ovaries with an embryo were found to be dispersed evenly all over the wheat spike, suggesting that within certain limits the developmental stage of ovaries and thus time of pollination within a spike are not as important as it was previously assumed.
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  • 36
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    European journal of plant pathology 104 (1998), S. 301-311 
    ISSN: 1573-8469
    Keywords: diagnosis ; fungi ; nucleic acid hybridization ; maize ; polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The fungus Fusarium moniliforme infects a wide range of crops throughout the world. In maize (Zea mays L.) it causes seedling blight and root, stalk, and ear rots. A simple procedure that can be used to detect infection by F. moliliforme from infected plant tissues has been developed. A F. moniliforme genomic library was prepared and used to identify the recombinant clones containing fungal DNA sequences not hybridizing with the DNA of the host plant, maize. Based on the nucleotide sequence information obtained from the F. moniliforme pUCF2 genomic clone, specific oligonucleotides were designed and used as primers for in vitro DNA amplification by the polymerase chain reaction. An amplification product was obtained with F. moniliforme DNA preparations whereas no amplified DNA was detected with DNAs from other fungal pathogens, including various Fusarium species, or from the host plant. This PCR analysis was successfully employed to identify F. moniliforme directly from the mycelia that develop from naturally infected maize seeds, with no need to obtain pure fungal cultures for reliable diagnosis. The protocol can be used for the diagnosis of infected plants and soils in epidemiological studies of Fusarium diseases, for seed health testing, and for evaluation of susceptibility to colonization in commercial maize hybrids.
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  • 37
    ISSN: 1573-0867
    Keywords: Africa ; biogenic emission ; dry season ; dynamic chamber ; forest ; grassland ; groundnut ; maize ; Miombo typesavanna ecosystem ; nitric oxide ; pulsing ; sorghum ; wet season ; Zimbabwe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract During October to December 1994, we measured diel and seasonal variability in nitric oxide (NO) exchange by a southern African savanna ecosystem (Grasslands Research Station, Marondera, Zimbabwe). A set of automated dynamic chambers was installed in three sub-sites: Miombo forest, natural grassland, and cropped soils (maize, sorghum, groundnut). The latter received a single application of commercialy available in-organic or cowdung fertilizer. The dynamic chamber system is described in detail, including correction of the resulting data for wall losses and gas-phase reactions of the NO-NO2-O3 triad. During the dry season, net vertical NO fluxes were close to the detection limit (0.44 ngN m-2s-1) regardless of vegetation type. During the transition from dry to wet season strong "pulsing" effects of the NO flux were observed: NO emissions increased by a factor of up to 60 within hours. During the wet season, NO emissions varied between the different sub-sites of the ecosystem; corresponding means are in ranking order of fertilized agricultural plots (27.2 ngN m-2 s-1), unfertilized agricultural plots (8.5 ngN m-2 s-1 ), grassland (4.4 ngN m-2 s-1 ), and Miombo forest (1.5 ngN m-2 s-1). Soil moisture was found to be the dominant factor controlling the NO fluxes. Whenever it was not limiting, the diel behaviour of NO emission followed the daily variation of soil temperature measured 1 cm below the surface.
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  • 38
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    Molecular breeding 3 (1997), S. 351-357 
    ISSN: 1572-9788
    Keywords: lignin ; bm3 ; maize ; O-methyltransferase ; PCR ; deletion ; brown midrib
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The caffeic acid O-methyltransferase (COMT) gene plays an important role in the synthesis of lignin. We have used the polymerase chain reaction in conjuction with genomic analysis to characterize deletion mutations of this gene in maize. In addition, we have analyzed and compared regions of the COMT gene from three distinct heterotic groups. Both PCR and Southern analysis indicate that the active wild-type COMT gene can be polymorphic. We suggest that the intron domain of at least one heterotic inbred can contribute to the alteration of the wild-type gene. In addition, multiple deletion mutations have occurred at this locus. We have found a previously uncharacterized deletion mutation in which segments of both the intron and exon have been deleted and replaced by other sequences. Precise knowledge of its sequence has allowed us to develop an assay by which we can follow this mutation in a breeding program.
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  • 39
    ISSN: 1572-9788
    Keywords: Alachlor ; herbicide tolerance ; maize ; RFLP ; SSR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to detect the genetic architecture of maize tolerance to Alachlor, a widely used chloroacetanilide, linkage analysis between the expression of the trait and allelic composition of molecular markers was performed. The experiment was carried out on a population of 142 recombinant inbred lines, developed starting from the F1 between two lines with different reactivity to the herbicide, and self-fertilized for 10 generations; the lines were typed by 48 RFLP markers and 66 microsatellites (SSR). Besides seedling tolerance, evaluated as proportion of normal (non-injured) plants after herbicide treatment, other minor components of tolerance were studied: seed germination ability, pollen germination and tube growth in the presence of the herbicide. The analysis, performed by three statistical methods, revealed the presence of factors controlling seedling tolerance on seven chromosomal regions. Five QTLs appeared to be involved in seed germination ability in the presence of Alachlor, four QTLs in pollen tolerance in terms of germination and four in tube growth under stress were detected. Three loci, on chromosomes 1, 7 and 10, explained most of the variation of seedling tolerance, thus being interesting candidate for marker-assisted selection.
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  • 40
    ISSN: 1573-5060
    Keywords: leafy normal-stature ; leafy reduced-stature ; non-leafy normal-stature ; non-leafy reduced-stature ; maize ; short season environments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Development of maize (Zea mays L.) types that produce leaf area rapidly and finish vegetative development quickly would increase production of maize in mid- to short-season areas. The Leafy (Lfy1) and reduced-stature (rd1) traits each make contributions to this end. However, these two traits have not previously been combined. Our objective was to evaluate the morphological aspects of non-leafy normal-stature (NLNS), leafy reduced-stature (LRS), non-leafy reduced-stature (NLRS), and leafy normal-stature (LNS) maize inbreds. Two traits, Lfy1 and rd1, were incorporated into a series of inbreds, resulting in a range of canopy architectures. Twelve variables were recorded for each of 30 inbreds over three years. The 12 variables were: seed emergence, above-ear leaf number, below-ear leaf number, dead leaf number at tasselling, live leaf number at tasselling, total leaf number, above-ear leaf area, ear leaf length, ear leaf width, ear height, internode length, and plant height. Inbreds containing the Lfy1 trait had more above-ear leaf area, above-ear leaf number, dead leaf number at tasselling, total leaf number and number of live leaves at tasselling than non-leafy inbred lines. Below-ear leaf number was not different among LRS, LNS, and NLNS inbred lines. LRS and NLRS inbred lines were also not different for below-ear leaf number. Plant height, ear height, and ear leaf length and width were higher in normal-stature than reduced-stature plants. The proportion of the seeds which emerged was higher for LRS inbreds than the other trait groups.
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  • 41
    ISSN: 1573-5060
    Keywords: leafy normal-stature ; leafy reduced-stature ; non-leafy normal-stature ; non-leafy reduced-stature ; maize ; short season environments ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Development of maize (Zea mays L.) types that produce leaf area and mature quickly would increase production of maize in mid- to short-season areas. The leafy (Lfy1) and reduced-stature (rd1) traits both make contributions to this end. However, these two traits have not previously been combined. Our objective was to evaluate the yield and yield components of non-leafy normal-stature (NLNS), leafy reduced-stature (LRS), non-leafy reduced-stature (NLRS), and leafy normal-stature (LNS) maize inbred lines. The two genes, ‘Lfy1’ and ‘rd1’, were incorporated into a series of inbred lines resulting in a range of canopy architectures. Ten variables were recorded for each of 30 inbred lines over three years. The 10 variables were: corn heat unit requirement from planting to tasselling, corn heat unit requirement from planting to silking, days between tasselling and silking, grain moisture content, husk dry weight, cob dry weight, ear length, maximum ear circumference, grain yield and ratio of grain yield to moisture content. Reduced-stature inbred lines reached anthesis more quickly than normal-stature inbred lines. Grain moisture content was less in reduced-stature inbred lines than normal stature trait groups. Leafy-reduced stature plants had the highest ratio of grain to moisture content and the lowest grain moisture content at harvest. Inbred lines containing the rd1 trait matured more rapidly than other trait groups. The LRS trait group yielded more than the other groups, and showed great potential for use in mid- to short-season environments.
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  • 42
    ISSN: 1573-5060
    Keywords: water stress ; breeding ; adaptation ; G × E ; clustering ; ordination ; Zea mays ; corn ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ten trials evaluated the performance of several late tropical maize populations (La Posta Sequía, Pool 26 Sequía and Tuxpeño Sequía) selected for tolerance to drought during flowering and grain filling and also for yield potential. Families (S1 or full-sib) had been selected recurrently for six to eight years on an index of traits. Pattern (clustering and ordination) analysis was used to analyse the relative performance of entries that included cycles of selection for drought tolerance in the populations and non-drought tolerant checks. Mean environment (E) yields ranged from 1.0 to 10.4 t ha-1. Analysis of variance showed that 97.9% of the total sums of squares was accounted for by E, and that, of the remaining sums of squares the G × E (genotype by environment interaction) was almost 3 times that of the contribution of G alone. Cluster analysis separated the checks, the earlier maturing drought tolerant entries and the later maturing drought tolerant entries. This was verified by principal component (PC) analysis of the G × E matrix. Grouping of the environments (i.e. based on entry performance), resulted in the separation of different types of droughts, and of medium and high yielding well-watered environments. The patterns of discrimination observed indicated that the yield gains under drought would have been unlikely to occur if selection had been done only in well-watered environments. Within each population, selection improved broad adaptation (higher mean yield) to both drought and well-watered environments and cycles of selection ‘jumped’ from non-drought-tolerant to drought-tolerant groups as their specific adaptation to drought environments increased.
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  • 43
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    Plant and soil 188 (1997), S. 319-327 
    ISSN: 1573-5036
    Keywords: agroforestry ; fallow ; maize ; nitrate ; root distribution ; root length density ; root to shoot ratio ; Sesbania sesban
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract One hypothesis for a benefit of integrating trees with crops is that trees with deep root systems can capture and “pump up” nutrients from below the rooting zone of annual crops. Few studies have compared both root and nutrient distribution for planted trees, crops and grassland vegetation. A field study was conducted on a Kandiudalfic Eutrudox in the highlands of western Kenya to measure rooting characteristics and distribution of inorganic N and water in three land-use systems (LUS): (i) Sesbania sesban (L.) Merr. fallow, (ii) uncultivated natural weed fallow and (iii) unfertilized maize (Zea mays L.) monoculture. The maximum rooting depth was 1.2 m in the maize LUS, 2.25 m in a 13-month-old natural fallow, and 〉 4 m in a 15-month-old sesbania fallow. Total root length was 1.26 km m-2 for the maize LUS, 5.98 km m-2 for the natural fallow, and 4.56 km m-2 to 4 m for the sesbania fallow. Root length to 1.2 m was greater (p 〈 0.01) for natural fallow than for maize and sesbania fallow. A considerable portion of the sesbania root length to 4 m was in the subsoil; 47% was at 1.2 to 4 m and 31% was at 2.25 to 4 m. Deep rooting of sesbania coincided with lower soil water below 2 m in the sesbania fallow than the natural fallow. Nitrate-N, but not ammonium-N, to 4 m was affected by LUS. Total nitrate to 4 m was 199 kg N ha-1 for the maize LUS, 42 kg N ha-1 for the natural fallow and 51 kg N ha-1 for the sesbania fallow. Soil nitrate in the maize LUS was highest at 0.3 to 1.5-m depth on this Oxisol with anion sorption capacity. No such accumulation of subsoil nitrate was present under sesbania and natural fallow.
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  • 44
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    Plant and soil 192 (1997), S. 37-48 
    ISSN: 1573-5036
    Keywords: Al toxicity ; Ca deficiency ; maize ; phosphogypsum ; root growth ; subsoil acidity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The chemical barrier to root development existing in the subsoils of acid soils is a subject of increasing interest. In order to better understand the factors involved in the amelioration of subsoil acidity, the effects of calcium sulphate, phosphogypsum and calcium carbonate on the properties of the solid and liquid phases of subsoil samples and on the growth and nutrient uptake by maize (Zea mays L.) were evaluated. The soils used were two alic red-yellow latosols, two acric dusky red latosols and one alic dark-red latosol from the State of São Paulo, Brazil. A vertical split-root technique was used in a greenhouse experiment, with the plants initially grown in a small pot with 130 g fertile soil, which was introduced in a larger pot containing 2 dm3 of the subsoil samples. The treatments consisted of a control (C) and applications of calcium carbonate (CC), calcium sulphate (CS) and phosphogypsum (PG) at the rate of 10 mmolc Ca2+ dm-3. CS and PG reduced soil acidity, but in a much smaller proportion than CC. Calcium carbonate reduced the activity of Al3+ because of the increase in pH. Total aluminum and calcium contents in the soil solution were much higher for the red-yellow latosols than for the other soils, indicating lower sorption of Ca2+ and $$SO_4^{2 - } $$ in these soils. The activity of Al in the soil solution was decreased in different ways for the five soils, depending on the ionic strength and the formation of the ionic pair $$AlSO_4^ + $$ and, in the case of PG, the formation of complexes of Al with F (AlF2+, $$AlF_2^ + $$ and $$AlF_3^\bigcirc $$ ). The subsoil samples presented severe restrictions for maize root growth and all three treatments were equally effective in increasing root development, which could be attributed to the supply of calcium in one of the acric dusky red latosols and a combined effect of the amendment in reducing the activity of Al and increasing the activity of Ca in the soil solution in the other soils. As a consequence the three treatments increased in the same manner water, N and K uptake from the subsoil and the dry matter production of maize. It can be concluded that, for the soils considered in this research, phosphogypsum is an effective amendment for acid subsoils containing low calcium or toxic aluminum contents.
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  • 45
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    Agroforestry systems 37 (1997), S. 199-207 
    ISSN: 1572-9680
    Keywords: groundnut ; humid lowlands ; labour efficiency ; maize ; partial budgeting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A fallow management trial was initiated in March 1990 in Yaounde, Cameroon, with the objective of identifying an efficient method of managing residue derived from a planted fallow of Cajanus cajan. Ten months after establishment, the shrubs were slashed and residues were treated in one of the following ways: burnt, incorporated in the soil, mulched on soil surface or removed. Following that, maize and groundnut were planted. Soil organic matter was fractionated after the residue treatments, and elementary partial budgeting was conducted. After three cycles of fallow and cropping seasons, maize yield was similar, about 3 t ha-1, in all plots except in the residue-removed plots, where it was 1.4 t ha-1. Yield trend of groundnut was also similar. The residue management method did not affect either the nature of fractions or the total content of soil organic matter. The highest net return, US$5945 ha-1 year-1, was obtained from the residue incorporated treatment, whereas the highest return to labour, US$11 per manday-1 was associated with burning of residues. In areas such as the forest zone of Cameroon where labour is a major constraint and climatic conditions allow vigorous vegetation growth, burning appears to be the best method of residues management, at least in the short run.
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  • 46
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    Plant growth regulation 21 (1997), S. 51-58 
    ISSN: 1573-5087
    Keywords: abscisic acid (ABA) ; ABA metabolism ; leaf water status ; maize ; Commelina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Metabolism and distribution of xylem-fed ABA were investigated in leaves of maize (Zea mays) and Commelina communis when water stress and xylem pH manipulation were applied. 3H-ABA was fed to excised leaves via the transpiration stream. Water stress was applied through either a previous soil-drying before leaves were excised, or a quick dehydration after leaves were fed with ABA. Xylem-delivered ABA was metabolised rapidly in the leaves (half-life 0.7 h and 1.02 h for maize and Commelina respectively), but a previous soil-drying or a post-feeding dehydration significantly extended the half-life of fed ABA in both species. In the first few hours after ABA was fed into the detached leaves, percentages of applied ABA remaining unmodified were always higher in leaves which received water stress treatments than in control leaves. However the percentage decreased to below the control levels several hours later in leaves which received a previous soil-drying treatment prior to excision, but had then been rehydrated by the xylem-feeding process itself. One possible explanation for this could be a changed pattern of compartmentalisation for xylem-carried ABA. A post-feeding dehydration treatment also changed the distribution of xylem-fed ABA within the leaves: more ABA was found in the epidermis of Commelina leaves which had been dehydrated rapidly after ABA had been fed, compared to the controls. The levels of xylem-delivered ABA remaining unmodified increased as the pH of the feeding solution increased from 5 to 8. The results support the hypothesis that water stress and a putative stress-induced xylem pH change may modify stomatal sensitivity to ABA by changing the actual ABA content of the leaf epidermis.
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  • 47
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    Euphytica 98 (1997), S. 141-148 
    ISSN: 1573-5060
    Keywords: maize ; corn ; floury ; open-pollinated ; white ; Zea mays ; landraces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Thirty-five white maize (Zea mays L.) landraces were obtained by the Indian Agricultural Program of Ontario (IAPO) from native farmers in Ontario and New York State between 1986 and 1989. These landraces probably belong to the racial complex Northern Flints and Flours. The 35 landraces, one experimental population and one control hybrid were evaluated in field experiments at two sites in 1993 and 1994. Data were collected on 24 traits in order to characterize and classify the IAPO landraces. Most quantitative traits examined exhibited considerable variation among the IAPO landraces. Variation was also observed within many of the landraces for ear and cob colour, and for endosperm texture. Most ears had eight rows of kernels, but there was some variation for this among and within landraces. Based on seven traits from the two sites in 1994, the IAPO population was grouped into 10 clusters by centroid clustering analysis.
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  • 48
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    Plant and soil 196 (1997), S. 295-300 
    ISSN: 1573-5036
    Keywords: diurnal rhythm ; maize ; nitrate uptake ; root age ; root zones ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The close relationship between nitrate depletion of the subsoil and root-length densities found in field experiments could not be explained by mathematical models simulating nitrate uptake (Wiesler and Horst, 1994). The objective of the present study was the validation of some of the assumptions made in these models namely uniform nitrate-uptake rates (NURs) independent on root age and daytime. Different techniques were developed and compared for the measurement of NUR of different root zones: (i) isolated root segments, (ii) compartmented uptake cuvettes, (iii) depletion of nitrate (water) from agarose blocks placed on specific zones of roots growing in nutrient solution and (iv) in rhizotrones filled with soil over the whole growing cycle of maize plants. All methods yielded a similar magnitude of NUR (10 - 30 pmol cm-2 s-1). However, only intact plants growing in nutrient solution as well as in soil, but not isolated root segments, showed higher NUR at apical root zones compared to more mature branching root zones by a factor of 2 - 8. The NUR of the root apex was particularly sensitive to the nitrogen demand of the plant and the assimilate supply from the shoots as affected by light intensity. At suboptimal, but not at optimal light conditions during preculture, NUR was lower in the dark than in the light. As plants matured, NUR of soil grown plants became increasingly dependent on water uptake. But even if nitrate uptake by mass flow was subtracted from total nitrate uptake, mature roots showed a surprisingly high nitrate-uptake capacity. The results indicate that the formation of root-age classes with different NUR and the assumption of lower NUR at night could improve the modelling of nitrate uptake.
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  • 49
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    Integrated pest management reviews 1 (1996), S. 251-263 
    ISSN: 1572-9745
    Keywords: storage ; maize ; Prostephanus truncatus ; larger grain borer ; Kenya
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A 4-year project in Kenya to develop management strategies for the larger grain borer (Prostephanus truncatus) in smallholder maize stores is described. The entomological investigations included behavioural and ecological studies of pest activity in experimental and farmers' maize stores and the natural environment, the use of insecticides to protect maize stored as grain and cobs and the release of a biological control agent. The latter was a predatory beetle; this marks the first release of a biological control agent against a storage pest in East Africa. The operation of a national trapping network to assess the extent and future spread of the pest and predator and target control campaigns is outlined. Recommendations for the control of the stored product pests, resulting from these investigations, were tested for likely adoption and modification in the light of socioeconomic surveys and a cost-benefit analysis. A decision tree approach to managing stored product pests is suggested which allows extension workers and farmers to decide the necessity of pesticide application when assessing how best to protect stored maize.
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  • 50
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    Nutrient cycling in agroecosystems 46 (1996), S. 225-234 
    ISSN: 1573-0867
    Keywords: long-term experiment ; maize ; wheat ; fertilizers ; farm yard manure ; weedicide application ; yield sustainability ; zinc deficiency ; nutrient uptake ; cropping sequence ; organic carbon build-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Long-term field experiments play an important role in understanding the complex interactions of plants, soils, climate and management and their effects on sustainable crop production. A long-term fertilizer experiment with maize-wheat-cowpea (fodder) is in progress since 1971 at Punjab Agricultural University farm Ludhiana, India. The experimental result for the first 21 years showed that application of N alone or in combination with P did not produce as much maize and wheat grains as the application of N, P and K together. Eight years after the start of the experiment, the optimal levels of N, P and K application (100% NPK) were unable to sustain the similar (maize) yield level as before because of Zn deficiency. Whereas in FYM amended plots the Zn deficiency did not appear and the higher crop yields could be sustained. The chemical control of weeds could not sustain the maize productivity at the same level as the manual removal of weeds. It was concluded that the high level of crop production can be sustained with the application of N, P and K under intensive cropping system provided deficiency of any of the micronutrient does not crop up. The deficiency of Zn is most likely to occur in semi-arid light textured alluvial soils under intensive cropping without the addition of farm yard manure/organic manures. In maize based cropping systems, manual control of weeds may be preferred to the chemical one. Addition of FYM in conjunction with 100% NPK is most beneficial both from bio-physical and economic point of view.
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  • 51
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    Nutrient cycling in agroecosystems 46 (1996), S. 91-95 
    ISSN: 1573-0867
    Keywords: ammonium thiosulphate (ATS) ; maize ; N uptake ; phenological stages ; N-S fertilisation ; S deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ammonium thiosulphate solution, (ATS, (NH4)2S2O3, 12% NH4-N and 26% S), is a nitrogen-sulphur fertiliser which can also inhibit nitrification, inhibit area hydrolysis and also solubilize micronutrients in alkaline soils. A three year field study was conducted in northeastern Italy to compare the growth, yield, and nutrient uptake of irrigated maize (Zea mays L.) fertilised with 250 kg N ha-1 urea-ammonium nitrate solution (UAN, 30-0-0) or UAN plus ATS. Dry matter (DM) yield, sulphur (S) and nitrogen (N) uptake were measured at several growth stages. Grain was measured and analyzed at maturity. Maize grain yield and N uptake were increased respectively 30.6% and 42.2% in the first year by adding ATS to UAN. Adding 10% by weight ATS to UAN (22.8 kg S ha-1) increased grain yields by 1.9, 1.7 and 1.6t ha-1 for the three years of the study. To distinguish whether the response was due to S or other ATS attributes, ATS was compared to an equivalent amount of S from single superphosphate (SSP). Plots fertilised with ATS gave grain yields 0.5 and 1.2 t ha-1 greater than plots fertilised with equal rates of S from SSP in the last two years of the study. This added yield from ATS over SSP may have been due to beneficial effects of ATS on N or micronutrient availability or to the split application of the S from ATS.
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  • 52
    ISSN: 1573-0867
    Keywords: Agro-ecological zones ; (AEZ) ; cabbages ; economic optimum (fertilizer rates) ; fertilizer response ; GIS ; maize ; modelling ; N ; P ; potatoes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A comprehensive long-term fertilizer trial programme for annual crops was established in 1985 at 70 sites in the high and medium rainfall areas of Kenya testing N and P, farmyard manure and other critical nutrients. Sites were selected according to their representativeness regarding soils and climate in agro-ecological units. The fertilizer trial programme included all major food crops typical for the respective area, both monocropped and intercropped in the case of cereals. Economic optimum fertilizer rates were calculated taking into account input/output price ratios and value-cost ratios. The majority of sites showed responses to N or P, only at one site response to both N and P with a significant interaction was found. Price elasticity of crops such as potatoes and cabbages was higher than that of maize or sorghum. Critical soil P levels were determined for maize at 13 ppm P for modified Olsen extract and 32 ppm P for Mehlich I extract. Variability of rainfall was found to greatly influence viability of fertilizer recommendations. First attempts to extrapolate fertilizer recommendations from areas of representativeness to areas with similar conditions by means of GIS and modelling approaches are discussed.
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  • 53
    ISSN: 1573-0867
    Keywords: cassava ; fertilizer ; intercropping ; maize ; paleustalf
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted on a Paleustalf to examine the effect of fertilizer application and the possible yield limiting factors in a cassava-maize intercropping system. Application of N-fertilizer significantly increased maize grain and cassava root yields as well as N, P and K concentrations of both crops compared with application of non-N fertilizer or with the unfertilized controls. Intercropping, with or without fertilizer application, led to a decrease in the root yield and N, P and K concentrations of cassava, but had no significant effect on nutrient concentrations and grain yield of maize. Growth analysis revealed that from 20 weeks after planting to harvest in cassava and during the flowering to harvest in maize dry matter accumulation in both crops was similar in intercropping. However, during the same period, the mean crop growth rate in cassava was less than a fourth of the mean crop growth rate in maize. It was concluded that the main factor limiting total yield in a cassava-maize intercropping system is the depression of early cassava growth by vigorous maize component, which reduces the amount of assimilate allocated to cassava roots.
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  • 54
    ISSN: 1573-5036
    Keywords: lettuce ; maize ; phosphorus solubilization ; plant growth promoting rhizobacteria (PGPR) ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Rhizobium leguminosarum bv. phaseoli strains P31 and R1, Serratia sp. strain 22b, Pseudomonas sp. strain 24 and Rhizopus sp. strain 68 were examined for their plant growth-promoting potential on lettuce and forage maize. All these phosphate solubilizing microorganisms (PSM) were isolated from Québec soils. The plants were grown in field conditions in three sites having high to low amounts of available P. In site 1 (very fertile soil), strains R1 and 22b tended to increase the dry matter yield of lettuce shoots (p≤0.10). Lettuce inoculated with rhizobia R1 had a 6% higher P concentration (p≤0.10) than the uninoculated control. In site 2 (poorly fertile soil), the dry matter of lettuce shoots was significantly increased (p≤0.05) by inoculation with strain P31 and 24 plus 35 kg ha-1 P-superphosphate, or with strain 68 plus 70 kg ha-1 P-superphosphate. In site 3 (moderately fertile soil), the dry matter of maize shoots was significantly increased (p≤0.05) by inoculation with strain 24 plus 17.5 kg ha-1 P-superphosphate, or with strain P31 plus 35 kg ha-1 P-superphosphate. Inoculation with PSM did not affect lettuce P uptake in the less fertile soil in site 2. In site 3 with the moderately fertile soil, maize plants inoculated with strain R1 had 8% higher P concentration than the uninoculated control (p≤0.01), and 6% with strains P31 and 68 (p≤0.05). The results clearly demonstrate that rhizobia specifically selected for P solubilization function as plant growth promoting rhizobacteria with the nonlegumes lettuce and maize. The P solubilization effect seems to be the most important mechanism of plant growth promotion in moderately fertile and very fertile soils when P uptake was increased with rhizobia and other PSM.
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  • 55
    ISSN: 1573-5036
    Keywords: maize ; root growth model ; root mapping ; root spatial distribution ; root system ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Most existing water and nutrient uptake models are based on the assumption that roots are evenly distributed in the soil volume. This assumption is not realistic for field conditions, and significantly alters water or nutrient uptake calculations. Therefore, development of models of root system growth that account for the spatial distribution of roots is necessary. The objective of this work was to test a three dimensional architectural model of the maize root system by comparing simulated horizontal root maps with observed root maps obtained from the field. The model was built using the current knowledge on maize root system morphogenesis and parameters obtained under field conditions. Simulated root maps (0.45 × 0.75 m) of horizontal cross sections at 3 depths and 3 dates were obtained by using the model for a plant population. Actual root maps were obtained in a deep, barrier-free clay-loamy soil by digging pits, preparing selected horizontal planes and recording root contacts on plastic sheets. Results showed that both the number of cross-sections of axile roots, and their spatial distribution characterized with the R-index value of Clark and Evans (1954), were correctly accounted for by the model at all dates and depths. The number of cross-sections of laterals was also correctly predicted. However, laterals were more clustered around axile roots on simulated root maps than on observed root maps. Although slight discrepancies appeared between simulated and observed root maps in this respect, it was concluded that the model correctly accounted for the general colonization pattern of the soil volume by roots under a maize crop.
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  • 56
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    Plant and soil 186 (1996), S. 29-38 
    ISSN: 1573-5036
    Keywords: Acetobacter ; Azoarcus ; Hebaspirillum ; Klebsiella ; maize ; sugar cane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The development of nitrogen fixation in maize can be considered the “holy grail” of nitrogen fixation research. As nitrogen fertilization is one of the highest costs of corn production, the development of a symbiosis between diazotrophic bacteria and corn would be of enormous economic value. Such a discovery would also improve human health as it would decrease the amount of nitrate in ground water as well as in corn cultured for human consumption. Several proposals have been made toward this end. These include: a) the transfer of root nodulation genes from a legume to maize; b) the expression of the bacterial nif regulon in maize organelles; and c) the development of corn lines with the ability to accept fixed nitrogen from diazotrophs in the rhizosphere. All of these proposals have enormous technical problems to overcome such that the development of nitrogen-fixing corn in the near term has been considered unlikely. An alternative and less-technically challenging approach may be a thorough study of non-pathogenic bacterial endophytes that already inhabit the corn plant. The discovery of a nitrogen-fixing bacterial-sugar cane association by Döbereiner and coworkers in Brazil illustrates the enormous potential of endophytic bacteria to enhance grass biomass in the absence of nitrogen fertilizer. Döbereiner and coworkers have discovered diazotrophic strains of Acetobacter diazotrophicus and Herbaspirillum seropedicae in lines of sugar cane that were bred in the absence of nitrogen fertilizer. The Brazilian group has also demonstrated that sugar cane plants infected with these diazotrophs are capable of deriving all of their nitrogen needs from N2. Recently, the presence of non-pathogenic endophytic bacteria in corn has been shown. Based on this evidence and using the sugar cane paradigm as an example, investigators are working toward the discovery and analysis of diazotrophic endophytes in corn which includes the search for corn germplasm that would readily benefit from an association with these bacteria. Several diazotrophic endophytes have been identified in grass species that are members of the α-, β-, and γ-subclasses of the proteobacteria. Our understanding of the ability of these bacteria to enhance the growth of grasses through nitrogen fixation is only beginning to be explored but this approach is thought to be far less technically challenging than are other proposals to develop nitrogen fixation in maize.
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  • 57
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    Euphytica 89 (1996), S. 291-296 
    ISSN: 1573-5060
    Keywords: maize ; Zea mays ; phenotypic correlation ; genotype × environment interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study was conducted to determine the relationships among various agronomic traits in inbreds, hybrids and between inbreds and their progeny of maize in short season areas. Phenotypic correlations of each measured trait between as well as among hybrids and inbreds were not the same. The highest correlation coefficient (r=0.78) was obtained between days to anthesis and yield. The high yielding inbreds did not necessarily give rise to high yielding progeny. Given that days to anthesis plays an important role in two major traits (yield and moisture) for hybrids, an emphasis on optimum anthesis dates during inbred development could be a useful criterion for the prediction of hybrid performance in short season areas.
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  • 58
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    Euphytica 91 (1996), S. 89-97 
    ISSN: 1573-5060
    Keywords: anthesis-silking interval ; drought tolerance ; grain yield ; maize ; QTL ; RFLP ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Grain yield in the maize (Zea mays L) plant is sensitive to drought in the period three weeks either side of flowering. Maize is well-adapted to the use of restriction fragment length polymorphisms (RFLPs) to identify a tight linkage between gene(s) controlling the quantitative trait and a molecular marker. We have determined the chromosomal locations of quantitative trait loci (QTLs) affecting grain yield under drought, anthesis-silking interval, and number of ears per plant. The F3 families derived from the cross SD34(tolerant) × SD35 (intolerant) were evaluated for these traits in a two replicated experiment. RFLP analysis of the maize genome included non-radioactive DNA-DNA hybridization detection using chemiluminescence. To identify QTLs underlying tolerance to drought, the mean phenotypic performances of F3 families were compared based on genotypic classification at each of 70 RFLP marker loci. The genetic linkage map assembled from these markers was in good agreement with previously published maps. The phenotypic correlations between yield and other traits were highly significant. In the combined analyses, genomic regions significantly affecting tolerance to drought were found on chromosomes 1,3,5,6, and 8. For yield, a total of 50% of the phenotypic variance could be explained by five putative QTLs. Different types of gene action were found for the putative QTLs for the three traits.
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  • 59
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    Euphytica 92 (1996), S. 129-134 
    ISSN: 1573-5060
    Keywords: common bean ; correlations ; intercropping ; maize ; selection gains ; selection methods ; yields ; Phaseolus vulgaris ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Despite the growing industrialization, technification and transformation that is happening in the agriculture around the world, and despite that agricultural research has always concentrated its effort on sole crops, multiple cropping systems have historically been important for common bean production in tropical countries. The reasons for this fact, are economical and social, as well as biological. Bean breeders have always been questioned on their work, because the development of new varieties is usually done in sole crop, but the varieties are grown in either systems. This paper addresses a set of questions that are usually presented to the breeders, in light of the evidence obtained from many trials conducted in Brazil and in the U.S.A.: Will the genotypes bred for sole crop conditions, perform well when grown in intercrop; How different should a genotype be, for cultivation in intercropping compared to genotypes developed for sole crop conditions; Is there a need for special breeding programs for intercropping and How could a breeding program focus the question of multiple (associated) cropping?
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  • 60
    ISSN: 1573-5036
    Keywords: maize ; nitrate ; nitrogen fertilizer ; root zone ; soil solution ; soil solution sampler with looped hollow fiber (LHF-sampler) ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of inorganic nitrogen (N) fertilizer on the ionic composition of the soil solution under maize (Zea mays L.) was studied. A pot experiment was carried out with two treatments combined factorially, with or without N application (Ca(NO3)2; +N and −N treatments, respectively), and with or without plants. Three looped hollow fiber samplers were installed in each pot to sample soil solutions nondestructively from the root zone, seven times during the 50-day growth period. Plants were harvested on the 50th day, and their nutrient contents determined. Effects of N fertilizer on the soil solutions were observed by the first sampling, 2 days after sowing. The concentrations of Ca and NO3 − and electrical conductivity (EC) increased significantly in the +N treatments as direct effects of fertilizer application. In addition, the concentrations of Mg, K, Na and H+ also increased and that of P decreased significantly as indirect effects caused by the re-establishment of chemical equilibria. This suggested the greater supply as well as the greater possibility of leaching loss not only of NO3 − but also of Ca, Mg and K. In the treatments with plants, the concentrations of NO3 −, Ca, Mg and K decreased with time and pH increased significantly compared with the unplanted soil. The depletion of N in the soil solution roughly agreed with the amount of N taken up by the plant. The depletions of K from the soil solution amounted to less than 10% of the amount of the K taken up, suggesting intensive replenishment of K from exchange sites in the soil. Depletions of Ca and Mg were several times higher than the amounts taken up, indicating that the depletions resulted from the adsorption of the divalent cations by the soil rather than uptake by plants. Because NO3 − is hardly absorbed by exchange sites in soil and was the dominant anion in solution, it was concluded that NO3 − had a major role in controlling cation concentrations in the soil solution and, consequently, on their availability for uptake by plants as well as their possible leaching loss. ei]H Marschner
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  • 61
    ISSN: 1573-5036
    Keywords: barley ; chloride ; foliar absorption ; maize ; saline sprinkling ; salinity ; sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Above-canopy sprinkler irrigation with saline water favours the absorption of salts by wetted leaves and this can cause a yield reduction additional to that which occurs in salt-affected soils. Outdoor pot experiments with both sprinkler and drip irrigation systems were conducted to determine foliar ion accumulation and performance of maize and barley plants exposed to four treatments: nonsaline control (C), salt applied only to the soil (S), salt applied only to the foliage (F) and salt applied to both the soil and to the foliage (F+S). The EC of the saline solution employed for maize in 1993 was 4.2 dS m−1 (30 mM NaCl and 2.8 mM CaCl2) and for barley in 1994, 9.6 dS m−1 (47 mM NaCl and 23.5 mM CaCl2). The soil surface of all pots was covered so that in the F treatment the soil was not salinized by the saline sprinkling and drip irrigation supplied nutrients in either fresh (treatments C and F) or saline water (treatments S and F+S). Saline sprinkling increased leaf sap Na+ concentrations much more than did soil salinity, especially in maize, even though the saline sprinkling was given only two or three times per week for 30 min, whereas the roots of plants grown in saline soil were continuously exposed to salinity. By contrast, leaf sap Cl− concentrations were increased similarly by saline sprinkling and soil salinity in maize, and more by saline sprinkling than saline soil in barley. It is concluded that barley leaves, and to a greater extent maize leaves, lack the ability to selectively exclude Na+ when sprinkler irrigated with saline water. Moreover, maize leaves selectively absorbed Na+ over Cl− whereas barley leaves showed no selectivity. When foliar and root absorption processes were operating together (F+S treatment) maize and barley leaves accumulated 11–14% less Na+ and Cl− than the sum of individual absorption processes (treatment F plus treatment S) indicating a slight interaction between the absorption processes. Vegetative biomass at maturity and cumulative plant water use were significantly reduced by saline sprinkling. In maize, reductions in biomass and plant water use relative to the control were of similar magnitude for plants exposed only to saline sprinkling, or only to soil salinity; whereas in barley, saline sprinkling was more detrimental than was soil salinity. We suggest that crops that are salt tolerant because they possess root systems which efficiently restrict Na+ and Cl− transport to the shoot, may not exhibit the same tolerance in sprinkler systems which wet the foliage with saline water. ei]T J Flowers
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  • 62
    ISSN: 1573-5036
    Keywords: barley ; chloride ; foliar absorption ; maize ; salinity ; sodium ; sprinkler irrigation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Brief pre- and post-irrigation sprinkling treatments using freshwater were tested to determine if these practices could reduce the uptake of salts through leaves when saline water is used to sprinkler irrigate crops. Maize and barley were sprinkler irrigated 2 to 3 times per week for 30 min with saline water (4.2 dS m−1, 30 mmol L−1 NaCl and 2.8 mmoles L−1 CaCl2 for maize and 9.6 dS m−1, 47 mmoles L−1 NaCl and 23.5 mmoles L−1 CaCl2 for barley) in separate experiments with plants grown in pots outdoors. The soil surface of all pots was covered to prevent salinization of the soil by the sprinkling water. One half of the sprinkled plants was grown in nonsaline soil to study the effects of pre-wetting and post-washing when ion uptake was primarily through leaves. The other half of the sprinkled plants was grown in soil salinized by drip irrigation, in order to evaluate the effects of pre-wetting and post-washing when Na+ and Cl- uptake was through both leaves and roots. Post-washing with freshwater (5 min) reduced the leaf sap concentrations of Cl- in saline-sprinkled plants from 56 to 43 mmol L−1 in maize and from 358 to 225 mmol L−1 in barley (averages for plants grown in nonsaline and saline soil). Na+ concentrations in leaf sap were reduced from 93 to 65 mmoles L−1 (maize) and from 177 to 97 mmoles L−1 (barley) by the post-washing. Pre-wetting had a small effect on ion uptake through leaves, the only significant reduction in seasonal means being in leaf Na+ concentrations for plants grown in nonsaline soil. Pre-wetting and post-washing, when combined, reduced leaf Cl- concentrations to levels similar to those of nonsprinkled plants grown in saline soil; however, Na+ concentrations in leaves remained 3.5 times (maize) and 1.5 times (barley) higher than those of nonsprinkled plants. When pre-wetting and post-washing were not applied, sprinkled barley plants grown in saline soil had grain yields which were 58% lower than nonsprinkled plants grown in saline soil, but the reduction in grain yield was only 17% when the freshwater treatments were given. We conclude that a brief period of post-washing with freshwater is essential when saline water is employed in sprinkler irrigation. By comparison, the benefits from pre-wetting were small in these experiments. ei]T J Flowers
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  • 63
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    Plant and soil 184 (1996), S. 131-141 
    ISSN: 1573-5036
    Keywords: aerenchyma ; cryo-microscopy ; intercellular water ; maize ; root cortex ; solute diffusion ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract During a study of the diffusivity of sulphorhodamine G in the cortical apoplast of maize roots widely discrepant rates were found between different samples. In roots which had developed large aerenchyma spaces, the diffusion in some regions was very fast, indistinguishable from the rate in water. In other regions the rate was as much as 100 times slower. Examination of frozen intact roots with the cryo-scanning electron microscope showed the presence of liquid filling some of the aerenchyma spaces, while other spaces of the same root contained air. X-ray microanalysis of the liquid (for oxygen) showed that the liquid was water with few detectable ions. Similar liquid was present in small intercellular spaces within the spoke-like radial files of cells between the large spaces, or between remnants of collapsed cell walls at the edges of the large spaces. It is proposed that regions of roots with high diffusivity are those in which some of the aerenchyma spaces are filled with water. In seeking the origin of this liquid, the progress of aerenchyma formation could be followed in the frozen tissues. The first change observed in a group of contiguous cells was a loss of vacuolar solutes and of cell turgor. Next the walls broke apart and collapsed back onto the surrounding turgid cells leaving a volume of ion-poor liquid. The liquid was probably not that found in some aerenchyma spaces of the mature roots, because the final stage of space formation was a loss of the liquid, leaving an air filled cavity surrounded by a composite lining formed from the collapsed walls of the broken cells. It is likely that the liquid in the spaces of mature aerenchyma is exuded from the remaining living cortical cells at times when the root turgor is high. This would be consistent with several recent studies which have shown periodic exudation of water from the surface of turgid roots. The spasmodic occurrence of root cortex tissue with enhanced diffusivity would have important implications for the transport of nutrient ions across the root.
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  • 64
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    Plant and soil 178 (1996), S. 153-160 
    ISSN: 1573-5036
    Keywords: carbon flow ; maize ; rhizosphere ; root ; sugar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influx and efflux of sugar-C and the cycling of C within intact maize roots (Zea mays L.) was studied in sterile solution culture. Using metabolic inhibitors it was shown that roots could take up sugars against the concentration gradient probably via H+-ATPase dependent plasmalemma proton cotransporters. In contrast to this, no evidence was found for an ATPase mediated efflux of sugars from the root. All parts of the root were capable of taking up exogenous sugars. Examination of sugar exudation sites along the root slowed efflux at all locations, with the amount of efflux linearly correlated with internal cellular concentration. The results clearly indicated that the influxefflux mechanisms are linked both spatially, temporally and with respect to the sugars capable of transportation. The turnover of C within the root was found to be extremely rapid with turnover of the soluble sugar pool being 0.8 to 15 times daily depending on root spatial location. The results strongly suggest that the recapture of sugars from outside the root plays an important role in regulating the amount of C lost to the soil which in turn will reduce both pathogen attraction and the size of the rhizosphere microbial population and will also increase the plant's C efficiency.
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  • 65
    ISSN: 1573-5036
    Keywords: Key words ; bioavailability ; crop plants ; DTPA ; maize ; Ni uptake ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The presence of higher-than-normal quantities of nickel is one of the most general features of ultramafic soils and is often suspected as the reason for their infertility. This study on the bioavailability of Ni in ultramafic soils derived from peridotites in New Caledonia showed important variations depending on the position of the soil in the landscape. In piedmont and non hydromorphic colluvio-alluvial soils, Ni was poorly absorbed by cultivated plants. In contrast, crops species grown in the plain soils, especially those found in the colluvio-alluvial and plain soils subject to temporary reducing conditions, possessed very high and even toxic Ni concentrations. Extraction of Ni by DTPA 5 mM was an effective method of estimating Ni bioavailability in these soils. The regression equation developed with only DTPA-extractable ni explained 88% of the variability in tomato Ni concentration. Extractable Ni might originate from the association of Ni with primary alterable minerals, organic matter and goethite. ei]Section editor: L V Kochian
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  • 66
    ISSN: 1573-5036
    Keywords: maize ; net translocation rate ; micronutrient ; root zone temperature ; shoot base temperature ; shoot demand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of suboptimal root zone temperatures (RZTs) on net translocation rates from the roots to the shoots and the concentrations of Fe, Mn, Zn, and Cu were examined in maize grown in nutrient solution or soil. Plants were grown at 12 °C, 18 °C and 24 °C RZT. At each RZT, the growth-related shoot demand for nutrients was varied by independently modifying the temperature of the shoot base (SBT) including the apical shoot meristem. The net translocation rates of Mn and Zn from the roots to the shoots were reduced at low RZTs, irrespective of the SBT and of the substrate (soil or nutrient solution). Obviously, the net translocation rates of Mn and Zn at low RZT were mainly regulated by temperature effects on the roots and not by the chemical nutrient availability in the rhizosphere or by shoot growth rate as controlled by SBTs. When both RZT and SBT were reduced, the decrease in net translocation rates of Mn and Zn was similar to the decline in the shoot growth rate and concentrations of Mn and Zn in the shoot fresh matter were not greatly affected or were even increased by low RZT. However, at high SBT and low RZT in nutrient solution, the depressed net translocation rates of Mn and Zn combined with the increased shoot growth resulted in significantly decreased concentrations of Mn and Zn in the shoot, indicating that Mn and Zn may become deficient even at high chemical availability. By contrast to Mn and Zn, the net translocation rates of Fe and Cu at all RZTs were markedly enhanced by increased SBTs. Accordingly, the concentrations of Fe and Cu in the shoot fresh matter were not greatly affected by RZTs, irrespective of the SBTs. These results indicate that the ability of roots to supply Fe and Cu to the shoot was internally regulated by the growth related shoot demand per unit of roots.
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  • 67
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    Euphytica 87 (1996), S. 45-51 
    ISSN: 1573-5060
    Keywords: sweet corn ; Rp1 ; disease resistance ; plant breeding ; complex locus ; recombination ; Puccinia ; Zea mays ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Rp1 locus of maize is a complex rust resistance locus where multiple resistance genes are clustered. Rare recombination events between Rp1 genes or alleles can produce two or more detectable genes linked in coupling phase. Such ‘compound’ genes can then be manipulated as a single gene in breeding programs. Several compound Rp1 genes, each carrying two or three tightly linked resistance genes, were constructed to test their utility in controlling common rust. While none of the lines carrying single Rp1 genes were resistant to all of the characterized North American P. sorghi biotypes, most of the two component and all of the three component Rp1 complexes were resistant. The potential for utilization of compound resistance genes in other crop species is discussed.
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  • 68
    ISSN: 1573-5060
    Keywords: embryogenesis ; wheat ; maize ; Triticum aestivum ; Zea mays ; haploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Embryogenesis was analyzed in wheat × maize hybrids using paraffin sectioning. Embryogenesis in wheat × maize hybrids is different from that in self-pollinated wheat plants. Development of the embryo is not accompanied by the formation of an endosperm. The endosperm nuclei remain free in the cytoplasm, fail to advance into the cellular stage, and degenerate at a later time. The antipodal cells quickly degenerate in the fertilized ovaries of wheat × maize hybrids similar to self-pollinated ovaries. The antipodal cells remain normal in unpollinated ovaries. The pre-embryo will abort if it is allowed to develop on the plant, because of a nutritional shortage in the absence of an endosperm. Therefore, embryo rescue is necessary for haploid production from a wheat × maize hybrids. Haploid polyembryos were obtained from spikelet culture of wheat × maize hybrids. The formation of polyembryos is due to the cleavage of the pre-embryo and the effect of 2,4-D. The frequency of haploid embryo production and plant regeneration is affected significantly by maize genotypes, but not by wheat genotypes. The concentration of 2,4-D affects only the size of the embryo.
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  • 69
    ISSN: 1573-0867
    Keywords: alley cropping ; Leucaena hedgerows ; maize ; N-competition ; N-use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A two-year field experiment was undertaken on an Alfisol in the forest-savanna transition zone of southwestern Nigeria, to study the effect of alley cropping, root barrier, application of N fertilizer andLeucaena leucocephala (Lam.) de Wit. prunings, on N utilization by maize andLeucaena hedgerows. The trial was conducted using a split-plot design with three replications. Mainplot treatments were alley cropping withLeucaena leucocephala planted at 4m interhedgerow spacing and control (no hedgerows). Four subplot treatments were with and without addition of hedgerow prunings, and with and without presence of root barriers. There were no significant effects of presence ofLeucaena hedgerows and root barriers on maize grain yield.Leucaena hedgerows recovered about 7% of the 30 kgN/ha applied as (15NH4)2SO4 to maize during one year. Application of hedgerow prunings increased maize yield by 82% and N-uptake in the grain by 50% over the treatment without prunings. Recoveries of15N-labelled prunings and fertilizer N by maize plants were about 10 and 16% respectively. Prunings addition increased15N-fertilizer recovery in main season maize and the residual value during the minor season by about 36% over the treatment without prunings. Pruning N-use efficiency was higher for plants grown adjacent to the hedgerows than in the middle of alleys during main season and the reverse was observed during the minor season. Absence of a root barrier increased N-use from prunings. Large amounts of applied N in the system were unaccounted for. Results of the trial showed, that without root barrier there was no measurable below-ground N competition betweenLeucaena hedgerows and maize.
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  • 70
    ISSN: 1573-0867
    Keywords: China ; Loess Plateau ; maize ; 15N ; Nitrogen ; urea ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field trials were carried out to study the fate of15N-labelled urea applied to summer maize and winter wheat in loess soils in Shaanxi Province, north-west China. In the maize experiment, nitrogen was applied at rates of 0 or 210 kg N ha−1, either as a surface application, mixed uniformly with the top 0.15 m of soil, or placed in holes 0.1 m deep adjacent to each plant and then covered with soil. In the wheat experiment, nitrogen was applied at rates of 0, 75 or 150 kg N ha−1, either to the surface, or incorporated by mixing with the top 0.15 m, or placed in a band at 0.15 m depth. Measurements were made of crop N uptake, residual fertilizer N and soil mineral N. The total above-ground dry matter yield of maize varied between 7.6 and 11.9 t ha−1. The crop recovery of fertilizer N following point placement was 25% of that applied, which was higher than that from the surface application (18%) or incorporation by mixing (18%). The total grain yield of wheat varied between 4.3 and 4.7 t ha−1. In the surface applications, the recovery of fertilizer-derived nitrogen (25%) was considerably lower than that from the mixing treatments and banded placements (33 and 36%). The fertilizer N application rate had a significant effect on grain and total dry matter yield, as well as on total N uptake and grain N contents. The main mechanism for loss of N appeared to be by ammonia volatilization, rather than leaching. High mineral N concentrations remained in the soil at harvest, following both crops, demonstrating a potential for significant reductions in N application rates without associated loss in yield.
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  • 71
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    Nutrient cycling in agroecosystems 46 (1996), S. 205-213 
    ISSN: 1573-0867
    Keywords: available potassium ; isotopic exchange ; leaching ; maize ; sustainability ; Togo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The transfers of native and applied K in a rhodic Ferralsol were studied in an agrosystem of southern Togo to propose sustainable cultivation strategies for K in kaolinitic soils. Potassium balance was measured over two years in field conditions under continuous maize cultivation with two K fertilisation levels (0 and 137 kg K ha−1 yr−1). Postassium leaching below the root zone, determined using ceramic cup samplers and Darcy's law, was on average 7.5 kg K ha−1 yr−1 with K fertilisation, i.e. 2% of the quantity of K applied, and 4.5 kg K ha−1 yr−1 without. The low leaching values resulted from a K concentration lower than 130 μM in the soil solution. The low K concentration in the soil solution was related to selective adsorption of K increased by a low content of exchangeable K, with a Gapon selectivity coefficient ranging from 7.9 and 11.5 M −0.5. So the level of exchangeable K must first be increased to raise K concentration in the soil solution. The fixation and release of K was analysed using the isotopic exchange method with 42K-ions and compartmental analysis of the kinetics of isotopic exchange. Potassium fixed in a form non available within one year accounted for 78% of the difference between the two treatments. The annual amount of K fertilisation must thus be based on the quantity of K removed in the grain and crop residues, with an extra addition to account for K fixation. Given a crop residue content of 85 kg K ha−1 yr−1 in the fertilised treatment, the return of crop residues is essential if sustainability is to be achieved with traditional cropping systems where little K fertiliser is added.
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  • 72
    ISSN: 1572-9680
    Keywords: beans ; hedgerow ; maize ; net present value ; partial budgeting ; sensitivity analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Although alley cropping has been shown elsewhere to permit continuous cropping, it has not been widely tested in the highlands of east and central Africa where it has the additional potential of controlling soil erosion. The effect of four rates (0, 30, 60 and 90 kg N ha−1) of inorganic N on the performance of alley cropping using Leucaena diversifolia as the hedgerow species was studied in the central highlands of Burundi. Significant increase in maize yield (average of 26%) due to alley cropping was only first realised in 1992, three years after the commencement of the trial. In 1993, the average yield advantage of the alley cropping plots was 21%. The prunings augmented the response of maize yield to inorganic N in 1992 and 1993. Compared with the control, economic benefits over the five-year period for all the treatments were negative.
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  • 73
    ISSN: 1572-9680
    Keywords: credit ; economics ; labour ; maize ; soil erosion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil erosion in the Philippine uplands is severe. Hedgerow intercropping is widely advocated as an effective means of controlling soil erosion from annual cropping systems in the uplands. However, few farmers adopt hedgerow intercropping even in areas where it has been vigorously promoted. This may be because farmers find hedgerow intercropping to be uneconomic compared to traditional methods of farming. This paper reports a cost-benefit analysis comparing the economic returns from traditional maize farming with those from hedgerow intercropping in an upland community with no past adoption of hedgerows. A simple erosion/productivity model, Soil Changes Under Agroforestry (SCUAF), is used to predict maize yields over 25 years. Economic data were collected through key informant surveys with experienced maize farmers in an upland community. Traditional methods of open-field farming of maize are economically attractive to farmers in the Philippine uplands. In the short term, establishment costs are a major disincentive to the adoption of hedgerow intercropping. In the long term, higher economic returns from hedgerow intercropping compared to open-field farming are realised, but these lie beyond farmers′ limited planning horizons.
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  • 74
    ISSN: 1573-5060
    Keywords: anthocyanin ; candidate gene ; linkage ; maize ; RFLP ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Linkages between molecular markers and genes involved in the expression of agronomical traits have already been described in all of the major crops. In most cases, the genetic model underlying the Quantitative Traits Loci (QTL) is discussed. Here, Restriction Fragment Length Polymorphisms (RFLPs) and Mapmaker-QTL have been used to pinpoint seven regions of the genome significantly correlated with four pigmentation qualitative traits of maize (Zea mays L.). Two of these, located on chromosomes 2 and 10, explain most of the variation of these traits. The R and B gene loci known to be involved in the regulation of the anthocyanin pathway map to the same regions and we suggest that these loci could be the candidate genes involved in the correlations detected with RFLPs. This type of result is in accordance with the hypothesis of the candidate gene which supposes that, if we have a very high density map of randomly-selected cDNA clones, it should theoretically be possible to associate a cloned genic sequence with a phenotypic trait where correlations are found.
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  • 75
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    Euphytica 92 (1996), S. 241-247 
    ISSN: 1573-5060
    Keywords: earliness ; selection ; adaptation ; exotic germplasm ; maize ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The maturity rating of maize (Zea mays L.) grown in Galicia (Northwestern Spain) varies from FAO 200 to 600. Later germplasm has usually too much moisture content in the kernel at normal harvesting time so serious problems with storing could appear. Besides, farmers have tended to use earlier varieties in the last few years. All this imposes limitations on the amount of germplasm available to develop varieties adapted to this area. To study the possibility of adapting late, exotic material to the environment of the Atlantic coast of Galicia we carried out six cycles of individual selection on two non-adapted populations of maize (Purdue A and Purdue B). The criterion of selection was early silking and the criterion of response was moisture content of kernel at harvesting. The original populations and the populations obtained after each cycle of selection were crossed to the hybrids CM105 × CM109 (tester Reid) and H99 × H95 (tester Lancaster) and were evaluated in four environments to study the efficiency of the selection scheme. There were significant reductions in days to silking (6.1 and 6.7 days from cycle 0 to cycle 6 for Purdue A and Purdue B, respectively) and grain moisture at harvesting (3.0 and 3.9% from cycle 0 to cycle 6 for Purdue A and Purdue B, respectively). There were also reductions in plant height and yield in both populations. In general, the crosses ‘population × Lancaster’ were higher for yield than the crosses ‘population × Reid’. Yield of the population crosses by both testers decreased after the six cycles of selection probably because of the earlier maturity of the selected populations. Some inbreeding depression may also have occurred.
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  • 76
    ISSN: 1573-5036
    Keywords: biomarker ; copper ; (iso)peroxidase ; maize ; peroxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Changes in the growth parameters and in enzyme activities were studied in roots and leaves of 14-days old maize grown in a nutrient solution containing various copper concentrations (i.e. 0.01 to 10 μM). A significant decrease in root and leaf biomass was only found at 10 μM Cu. In contrast, changes in several enzyme activities occured at lower copper concentrations in the solution, corresponding to different threshold values which are lower than those observed for growth parameters. Peroxidase (POD) activity significantly increased in all investigated plant organs (i.e. 3rd-leaf, 4th-leaf and roots) in relation to their copper content. Additionally, glucose-6-phosphate dehydrogenase (G-6-PDH), and isocitrate dehydrogenase (ICDH) activities decreased in the leaves, especially in the 4th-leaf. However, the activity of malic enzyme (ME), G-6-PDH, glutamate dehydrogenase (GDH) and ICDH increased with the copper content in roots. According to the relationship between POD activity and copper content, the toxic critical value was set at 26 mg Cu per kg dry matter (DM) in roots and 21 mg Cu per kg DM in the 3rd-leaf. In roots, a new isoenzyme of peroxidase appeared for copper content above 12.6 mg Cu kg DM−1. Measurement of enzyme activity, especially that of POD and Cu-specific changes in the (iso)peroxidase pattern, might be used as biomarkers to assess the phytotoxicity for maize grown on copper-contaminated substrata.
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  • 77
    ISSN: 1573-0867
    Keywords: millet ; sorghum ; rice ; maize ; wheat ; nutrient harvest index ; post-anthesis nutrient uptake ; recovery fraction ; simulation modelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In land use plans, fertilizer recommendations are indispensable to avoid soil nutrient depletion or soil water pollution. Nutrient relations of five cereals have been evaluated on the basis of a literature review with the aim of arriving at such fertilizer recommendations at regional level. Nutrients considered were nitrogen, phosphorus and potassium for millet, sorghum, maize, rice and wheat. The relevant nutrient relations are fertilizer nutrient application to nutrient uptake, and nutrient uptake to crop yield. In addition, post-anthesis nutrient uptake is considered. Subsequently, obtained results are used in simulation modelling exercises to calculate the time required to attain an equilibrium nutrient balance and to investigate the effect of erosion control and straw recycling. Although fertilizer requirements could be assessed for each of the five cereals, monitoring of nutrient supply from natural sources remains necessary. Moreover, research on fertilizer use should focus on improvement of fertilizer recoveries and multiperiod models for both N and P uptakes by crops to allow quantitative land use planning where the time scale is included.
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  • 78
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    Nutrient cycling in agroecosystems 43 (1995), S. 83-86 
    ISSN: 1573-0867
    Keywords: phosphorus fertilizers ; phosphate rock ; maize ; winter rape
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract North Carolina Natural Phosphate (NCNP) is a natural marine sediment, excavated, washed and sold in unprocessed form as a phosphate fertilizer. In vegetation and field experiments carried on in Poland in 1992–1994 with potatoes, rye, maize and winter rape the efficiency of phosphorus in NCNP was in the range of 75–100% of phosphorus in superphosphate. Blending and granulating of NCNP with urea and sulphur proved to be very effective. Further works on blending technology and the usability of NPS or NPKS blends are recommended.
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  • 79
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    Nutrient cycling in agroecosystems 40 (1995), S. 41-48 
    ISSN: 1573-0867
    Keywords: Arachis hypogaea ; intercropping ; maize ; N2 fixation ; N-transfer ; 15N ; Vigna radiata ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Grain legumes are used widely in intercropping systems. However, quantitative and comparative data available as to their N2 fixation and N beneficial effect on the companion crop in intercropping systems are scarce. Hence, studies were conducted to ascertain the above when cowpea (Vigna unguiculata L.), mungbean (Vigna radiata L.) and groundnut (Arachis hypogaea L.) were intercropped with maize. The study was15N-aided and made outdoors in basins (30 L) filled with 38 kg of soil.15N labelling was effected by incorporating15N-tagged plant material or applying15N-labelled fertilizer along with sucrose to stabilize15N enrichment in the soil during the experimental period. Intercropped groundnut fixed the highest amount of nitrogen from the atmosphere (i.e. 552 mg plant−1), deriving 85% of its N from the atmosphere. Intercropped cowpea and mungbean fixed 161 and 197 mg N plant−1, obtaining 81% and 78% of their N content from the atmosphere, respectively. The proportion of N derived by maize from the associated legume varied from 7-11% for mungbean, 11–20% for cowpea and 12–26% for groundnut which amounted to about 19–22, 29–45 and 33–60 mg N maize plant−1, respectively. The high nitrogen fixation potential of groundnut in dual stands and its relatively low harvest index for N have apparently contributed to greater N-benefical effect on the associated crop.
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  • 80
    ISSN: 1573-8469
    Keywords: disease progress ; disease spread ; Exserohilum turcicum ; maize ; northern leaf blight ; plant density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effect of plant density (37,037, 44,444 and 55,555 plants/ha) on the increase of northern leaf blight in time and space on two maize cultivars planted at spacings of 90, 75 and 60 between rows and 30 cm within rows was investigated.Exserohilum turcicum infested maize residue was used as an inoculum source. Maize density did not significantly affect the disease indices, but significantly influenced the grain yield of the two cultivars. In contrast, the two cultivars differed significantly (P≤0.01) in disease severity, but not grain yield. Higher disease severities and grain yields were associated with higher plant densities, whereas the apparent infection rate was lower in higher plant density. Distance from the maize residue (inoculum source) significantly influenced disease severity. The percentage leaf area blighted, area under disease progress curve and disease progress curve intercept decreased with distance from the maize residue area, but the apparent infection rate on EV8429-SR appeared to increase with distance. Disease gradients (b) were higher in closely planted maize and flattened with time in one location only.
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  • 81
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    Plant and soil 172 (1995), S. 229-234 
    ISSN: 1573-5036
    Keywords: aluminium ; calcium ; maize ; nitrate ; roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Aluminium toxicity has been shown to decrease NO3 - uptake in Zea mays seedlings during the first 30 minutes after addition of Al3+. This suggests that NO3 - uptake inhibition could be a primary response to Al addition. We therefore tested the hypothesis that NO3 - uptake and root elongation are affected differently by Al3+. Eight-day old seedlings were exposed to 100 μM Al3+ in the presence of 1 or 10 mM Ca2+, added as either CaSO4 or CaCl2. In the presence of Al3+, cumulative uptake of NO3 --N during an 8 h period was not affected by Ca2+ level (1 or 10 mM). Root elongation at 1 mM Ca2+ was decreased to 63% of the control by the presence of Al3+. Raising ambient Ca2+ from 1 to 10 mM in the presence of Al3+ restored elongation rates to 78% (CaCl2) and 88% (CaSO4) of elongation without Al. Because reductions in root elongation were partially overcome by added Ca2+, but lowered uptake of NO3 - was not, it was concluded that Al3+ toxicity decreased root growth and NO3 - uptake by different mechanisms.
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  • 82
    ISSN: 1573-5036
    Keywords: maize ; organic acids ; rhizosphere ; root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influx and efflux of organic acids across the root-soil interface were investigated in intact, sterile maize (Zea mays L.) roots under a variety of experimental conditions. Under nutrient-sufficient conditions the efflux of organic acids was shown to constitute 〈 1% of the total C lost across the root-soil interface. Under severe nutrient stress, however, the rates of malate and citrate efflux from the root increased 33 and 12 fold respectively. Influx experiments indicated that roots could not directly reabsorb citrate-Fe3+ or other metal complexes from solution. Influx of citrate was observed only at high external citrate concentration (≥ 1 mM) or from solutions with low ionic strengths. It was postulated that citrate influx is of little importance in a soil environment.
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  • 83
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    Euphytica 82 (1995), S. 157-164 
    ISSN: 1573-5060
    Keywords: EPSP synthase ; glyphosate ; herbicide tolerance ; natural variation ; Zea mays ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Variation in susceptibility to the safe broad-spectrum herbicide glyphosate was investigated in maize. Eleven inbred lines, grown in a growth chamber, were evaluated for their tolerance to the herbicide at 2.4 mM (0.2 kg a.i. in 400 I ha-1 of water). Following treatment with glyphosate at the three-leaf stage, significant variation in damage, expressed as visual injury ratings scored 7, 14 and 21 days after the application of the herbicide, was found. Effects on dry weight and shoot height were consistent with visual scores and the carbon-exchange rate was found to be a sensitive index of differential injury. Biochemical characterization of 5-enol-pyruvyl-shikimate-3-phosphate (EPSP) synthase, the main target of the herbicide, ruled out the possibility that this differential susceptibility was due to variations in the sensitivity of the enzyme. On the contrary, a positive correlation was found between in vivo tolerance and EPSP synthase levels, measured at different stages during seedling growth. This result suggests that a naturally occurring difference in EPSP synthase levels in the tissues may contribute to the differential response observed in vivo in maize inbreds.
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  • 84
    ISSN: 1573-5036
    Keywords: alley cropping ; Chromolaena odorata ; Gliricidia sepium ; groundnut ; maize ; rice ; root length density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tree root systems may improve soil fertility through carbon inputs, uptake of leachable nutrients and maintenance of soil biomass, but can at the same time reduce crop yields by competition for water and nutrients. Quantitative information about the positive and negative effects of tree roots and their changes in space and time are necessary for the optimization of agroforestry associations. An alley cropping experiment was layed out as a randomized complete block design on a Plinthic Lixisol/Ferralic Cambisol with Gliricidia sepium hedgerows at 5 m distance, including a sole cropping control. The development of root systems was monitored by sequential soil coring (eight samplings) during one year, with maize and groundnut as crops. Additional information is presented from a single sampling for rice during the foregoing year. Pronounced fluctuations of live root length density indicated an important variability in the nutrient and water uptake capacity of the vegetation. At low total root length density, the hedgerows affected the root development in the agroforestry plots directly by the presence of their root systems. At high root length density, they affected root development mainly by improving crop root growth and influencing the composition of the spontaneous vegetation. The root length density of the hedgerows was too low to compete with the crops for soil resources. The hedgerows tended to increase root length densities in the subsoil when few roots were present, thus possibly reducing the risk of nutrient leaching. However, the length density of the perennial root systems decreased during the cropping season, presumably as an effect of repeated pruning, and attained minimum values almost at the same time as the crops. Trees with denser root systems which are less frequently pruned may be more efficient in achieving closer nutrient cycles, though at the cost of higher root competition with crops.
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  • 85
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    Plant and soil 171 (1995), S. 141-146 
    ISSN: 1573-5036
    Keywords: aluminium ; maize ; monomeric Al ; organo-Al complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract As aluminium (Al) severely inhibits magnesium (Mg) uptake by many plant species, Mg uptake and Mg-Al interactions in maize (Zea mays L.) were studied in a series of short and long-term experiments. A relationship between Mg uptake and the degree of Mg saturation of exchange or binding sites of the root apoplast (root-CEC) was studied by growing plants in solutions containing: (i) different concentrations of Al, calcium (Ca) and hydrogen (H) ions; and (ii) a number of organic complexes of Al. In short-term experiments, Ca had little effect on the Mg nutrition of maize plants. However, with increasing concentrations of Al and H ions in nutrient solution, there was a decrease in both the degree of Mg saturation of root-CEC and Mg uptake. Effects of pH on cation (H, Al, Mg, Ca) binding at the root apoplasm were pronounced and complicated because of a simultaneous change of H ion concentration, effective root-CEC and Al speciation. The behaviour of Al as organic Al complexes differed from that supplied as aluminium chloride (AlCl3). In the presence of organo-Al complexes, less Mg was replaced from apoplastic binding sites and Mg uptake was inhibited less severely than with AlCl3. In a long-term experiment, Al-citrate, in contrast with AlCl3, was not phytotoxic to maize, expressed by the lack of any inhibition of shoot biomass production.
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  • 86
    ISSN: 1573-5036
    Keywords: acidification ; Alfisol ; aluminum toxicity ; cowpea ; exchangeable acidity ; maize ; manganese toxicity ; nitrogen fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Increased use of N fertilizer and more intensive cropping due to the rising food demand in the tropics requires design and evaluation of sustainable cropping systems with minimum soil acidification. The objectives of this study were to quantify acidification of an Oxic Kandiustalf with different types of N fertilizer in two cropping systems under no-tillage and its effect on crop performance. Chemical soil properties in continuous maize (Zea mays L.) and maize-cowpea (Vigna unguiculata (L.) Walp) rotation were determined with three N sources (urea (UA), ammonium sulfate (AS) and calcium ammonium nitrate (CAN)) in Nigeria, West Africa, during five years. Chemical soil properties were related to grain yield and diagnostic plant nutrient concentrations. For the three N sources, the rate of decline in soil pH in maize-cowpea rotation was 57±7.5% of that in continuous maize, where double the amount of N fertilizer was applied. The rate of soil acidification during the five years was greater for AS than for UA or CAN in continuous maize, and not different for UA and CAN in both cropping systems. With AS, soil pH decreased from 5.8 to 4.5 during five years of continuous maize cropping. Exchangeable acidity increased with N fertilization, but did not reach levels limiting maize or cowpea growth. Return of residues to the soil surface may have reduced soluble and exchangeable Al levels by providing a source of organic ligands. Soil solution Mn concentrations increased with N fertilization to levels likely detrimental for crop growth. Symptoms of Mn toxicity were observed on cowpea leaves where AS was applied to the preceding maize crop, but not on maize plants. Soil acidification caused significant reductions in exchangeable Ca and effective CEC. Main season maize yield with N fertilization was lower with AS than with UA or CAN, but not different between UA and CAN during the six years of cropping. The lower maize grain yield with AS than with the other N sources was attributed to lower pH and a greater extractable Mn concentration with AS. When kaolinitic Alfisols are used for continuous maize cropping, even under no-tillage with crop residues returned as mulch, the soil may become acidifed to pH values of 5.0 and below after a few years. The no-till cereal-legume rotation with judicial use of urea or CAN as N sources for the cereal crop is a more suitable system for these poorly buffered, kaolinitic soils than continuous maize cropping. The use of AS as N source should be avoided. H Marschner Section editor
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  • 87
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    Plant and soil 176 (1995), S. 101-105 
    ISSN: 1573-5036
    Keywords: arbuscular mycorrhiza ; “criollo” ; growth ; inoculation ; maize ; mycorrhizal ; native ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We tested the effect of two single species inocula and a mixed inoculum of the native population of arbuscular mycorrhizal (AM) fungi on the growth response of “criollo” maize (Zea mays L.). To determine the inocula that produced the highest response on maize growth, we conducted a greenhouse experiment at 3 levels of P fertilization (0, 40 and 80 kg ha−1). Inoculation with Glomus mosseae (Nicolson and Gerdemann) Gerd. and Trappe (LMSS) produced the greatest shoot growth rates at the two lowest P fertilization levels. Inoculation with Acaulospora bireticulata Rothwell and Trappe (ABRT) and the native population (NP) resulted in similar shoot growth rates at all P levels. These rates were higher than the non-mycorrhizal control rate at the lowest P level but lower than the control at the highest P level. Also, ABRT and NP had significantly lower shoot growth rates than the inoculation treatment with G. mosseae at all P levels. The non-mycorrhizal control had the lowest growth rate at the lowest P level but its growth rate increased linearly with increased P fertilization. Inoculation with G. mosseae and A. bireticulata produced similar colonization rates which were lower than the native population colonization rate. There was no correlation between colonization and shoot growth rates.
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  • 88
    ISSN: 1572-9680
    Keywords: groundnut ; maize ; root biomass ; root turnover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A considerable amount of data is available about above-ground biomass production and turnover in tropical agroforestry systems, but quantitative information concerning root turnover is lacking. Above- and below-ground biomass dynamics were studied during one year in an alley cropping system withGliricidia sepium and a sole cropping system, on aPlinthic Lixisol in the semi-deciduous rainforest zone of the Côte d'Ivoire. Field crops were maize and groundnut. Live root mass was higher in agroforestry than in sole cropping during most of the study period. This was partly due to increased crop and weed root development and partly to the presence of the hedgerow roots. Fine root production was higher in the alleys and lower under the hedgerows compared to the sole cropping plots. Considering the whole plot area, root production in agroforestry and sole cropping systems was approximatly similar with 1000–1100 kg ha−1 (dry matter with 45% C) in 0–50 cm depth; about 55% of this root production occured in the top 10 cm. Potential sources of error of the calculation method are discussed on the basis of the compartment flow model. Above-ground biomass production was 11.1 Mg ha−1 in sole cropping and 13.6 Mg ha−1 in alley cropping, of which 4.3 Mg ha−1 were hedgerow prunings. The input of hedgerow root biomass into the soil was limited by the low root mass ofGliricidia as compared to other tree species, and by the decrease of live root mass of hedgerows and associated perennial weeds during the cropping season, presumably as a result of frequent shoot pruning.
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  • 89
    ISSN: 1573-5060
    Keywords: maize ; climatic factor ; genotype x environment interaction ; root trait ; statistical model ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The efficiency of genotopic and climatic characteristics in accounting for the interaction between genotypes and environments has been assessed in a three-year trial involving a set of genotypes presenting a range of root morphology characteristics (number and size). Climatic information on rainfall and temperature was recorded during the experiments, together with extra data on the growth and development of the genotypes. Their effects have been tested in factorial regression models. Climatic covariates were very powerful in accounting for the genotype by year interaction as well as the year main effect alone. For the number of adventitious roots on internode 7, the main effect of year could be described as a linear function of the average temperature and precipitation that occurred during the period of root initiation and growth. For internode 6, no clear conclusion was possible. For the root traits studied, 74 to 98% of the interaction could be explained by one climatic covariate. The regression coefficients can be considered as measures of genotypic stability. The genotypic covariates describing aerial development performed rather poorly, compared with environmental ones, even though the physiological and functional relationships between root and shoot are well known. Neither genotype main effect nor genotype by year interaction could be described sufficiently by factorial regression. Still, the genotypic covariates performing best clearly differed between root counts and size. Also the best genotypic covariates differed for main effect and interaction.
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  • 90
    ISSN: 1573-5060
    Keywords: germplasm registration ; maize ; Zea mays ; Isozyme ; markers ; morphological traits ; morphological variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetic markers (isozymes), in addition to the traits recommended by the UPOV convention, have been used in France since 1989 to characterize the maize inbred lines submitted to registration. In the years 1989. 1990 and 1991, a total of 974 inbreds has been described according to this procedure. Relationships between genetic markers were investigated and underlined the occurrence of linkage disequilibria within the tested germplasm. These disequilibria appeared to depend strongly on the breeding history of the germplasm. In some cases, these disequilibria could be related to a major progenitor (foundation effect). Relationships between genetic markers and quantitative traits were also pointed out. High coefficients of determination (up to 60%) were in some cases observed at the within group level, and also appeared to be very dependent on the major progenitors of the group of interest. The consequences of these results for breeding and distinctiveness studies are discussed.
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  • 91
    ISSN: 1573-5060
    Keywords: diallel cross ; qualitative interactions ; recurrent selection ; stability analysis ; maize ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A 10×10 diallel cross experiment involving white modified opaque-2 maize inbred lines was grown at four sites. A stability analysis, based on both a combining ability and a heterotic pattern model, was developed. The stability analysis provided valuable information on the genotype x environment interaction properties of the 10 inbred lines. The Gail & Simon (1985) test for qualitative interactions provided a means of determining the nature of these interactions. The inbred, SO507W(M), is shown to have the best potential for use in a hybrid breeding programme, in terms of having the highest weighted general combining ability and line heterosis, and the best general adaptability to all four sites. The single cross, SO713W(P) x PO558W(F), has been identified as one of the genotypes to be used in a recurrent selection programme that favours specific combining ability.
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  • 92
    ISSN: 1573-5060
    Keywords: genetic control ; maize streak virus ; major genes ; minor genes ; resistance ; Zea mays ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary S1 to S5 inbred lines, derived from a maize population bred for its overall resistance to three tropical viruses, were screened for resistance to maize streak virus (MSV) by artificial plant infection using viruliferous leafhoppers. Symptoms were rated and intra-line frequency distributions studied for all pedigree inbred lines. Mortality due to MSV was very low among these inbreds. Symptoms appeared later, developed slower and were less severe than in the susceptible control hybrid. Results of a study of 500 S1 and 93 S2 lines suggested that resistance is under genetic control via a system involving loci with major genes (with dominance for resistance) controlling high to complete resistance, associated with a genetic system involving loci with minor genes controlling partial resistance. Lines expressing complete resistance to MSV were developed from 5 cycles of inbreeding and selection. The relevance of such complete and partial resistance is discussed.
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  • 93
    ISSN: 1573-5060
    Keywords: glyphosate ; herbicide tolerance ; non-target effects ; somaclonal variation ; Zea mays ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The progeny of 104 regenerated maize plants were screened for tolerance to the safe broad-spectrum herbicide glyphosate during seed germination and early growth. Seven somaclones showed varying degrees of resistance to the application of the herbicide at 1.2 mM (0.1 kg a.i. in 400 1 ha-1 of water). Plants capable of a normal growth following treatment with 2.4 mM (0.2 kg ha-1) glyphosate at the three leaf stage were selfed, and their progeny analyzed. A family able to tolerate the exposure to glyphosate at 2.4 mM was isolated and shown to maintain a photosynthetic rate comparable with control after the application of the herbicide. The selfed progeny of the tolerant somaclone was characterized as to the properties of two targets of glyphosate, the shikimate pathway enzymes 5-enol-pyruvyl-shikimate-3-phosphate (EPSP) synthase and 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase. In vitro tests ruled out the possibility that the tolerance was due to altered forms of these enzymes. Families showed significant variability with regard to EPSP and DAHP synthase levels, measured at different stages during seedling growth; however, not even these traits were correlated with in vivo response to glyphosate. The possible role of other physiological processes in determining the increased tolerance to the herbicide is discussed.
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  • 94
    ISSN: 1573-5060
    Keywords: Zea mays ; insect vectors ; resistance ; maize ; maize streak virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Settling, probing and oviposition behaviour ofCicadulina mbila, the main vector of maize streak virus (MSV) disease were studied on four maize genotypes, 100MSR, HASR, Reunion and H512. Of the four, only H512 was completely susceptible to MSV while the other three genotypes were all known to have some resistance to MSV. Generally,C. mbila settled in higher numbers on the MSV susceptible genotype compared to the three virus resistant ones. However, the number of probing marks left on H512 and 100MSR were similar while more marks were made on Reunion and HASR in increasing order. The oviposition patterns were similar to those observed for probing behaviour. The results suggest the existence of potentially useful resistance mechanisms against the vector. The possible implications of these results are discussed.
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  • 95
    ISSN: 1573-5060
    Keywords: AMMI ; best linear unbiassed prediction ; factorial regression ; genotype by environment interaction ; maize ; missing values ; multiplicative interaction ; reduced rank regression ; two-way table
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary As the sequel to a paper that dealt with the theoretical aspects of linear and bilinear models for the analysis of genotype by environment interaction in multi-environment trials, this paper presents an illustration of the application of these models to real life data. The data come from maize trials that were conducted within the ongoing evaluation programme for the Dutch Descriptive Variety List of Field Crops. The variable that is analyzed is dry matter content. It is shown how linear and bilinear models can be used supplementary to each other within a general strategy for dealing with genotype by environment interaction.
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  • 96
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    Euphytica 84 (1995), S. 57-65 
    ISSN: 1573-5060
    Keywords: bush bean ; Phaseolus vulgaris ; intercropping ; maize ; sole cropping ; Zea mays ; quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The intereropping of common bean (Phaseolus vulgaris L.) and maize (Zea mays L.) is a common practice in the Northwest of the Iberian Peninsula. In this experiment, 25 populations of bush bean were evaluated for nutritional and culinary traits of dry seed and immature pod in sole crop and in intercrop in two years and in two locations, in order to determine whether selection of bush bean in sole crop can result in genetic progress for intercrop. There were no differences in the population means between cropping systems. Significant differences were detected among populations for all traits tudied except starch content, crude ash, water absorption and coat proportion. Population x environment interaction was significant for pod thickness. Bean populations did not interact with cropping systems for any trait, although there were differences between cropping systems for crude protein. These results suggest that a sole crop system provides sufficient information to select bean populations efficiently for the bush bean-maize intercropping system, although the advanced generations of the breeding program should be tested for quantitative traits such as protein content in the appropriate cropping system (intercropping) to know the competitive ability with the associated species. This would permit to choose bush bean populations which complement more efficiently and would be less competitive with the maize population.
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  • 97
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    European journal of plant pathology 101 (1995), S. 301-310 
    ISSN: 1573-8469
    Keywords: Bipolaris zeicola ; maize ; multilocus haplotypes ; Northern Corn Leaf Spot ; sexual reproduction ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Over 3 years 15 samples ofC. carbonum were collected from 11 corn fields in North Carolina and Tennessee. Among 514 isolates of race 2 and 319 isolates of race 3, five phenotypic characters (mating type, production of pseudothecia, production of asci and ascospores, tolerance to cycloheximide and carboxin, respectively) that are controlled by single genes at unlinked loci (Mat, Psu, Asc, Cyh, Crb) were examined. Gametic phase disequilibrium (GPD) was analyzed by three methods. First, observed and expected four-locus haplotype frequencies were compared inG-tests for goodness of fit (method 1) and second, four-locus disequilibrium was calculated using an index of association which is based on the variance of the number of loci at which two isolates in a given population differ (method 2). Third, observed and expected frequencies of allele pairs were compared in 2×2 contingency tables (method 3). Each test was performed on individual and pooled samples which also allowed assessment of heterogeneity among samples. In general this heterogeneity was low, i.e. the consistency of associations among samples was high. Four-locus disequilibrium was significant in race 2 with method 1 but not with method 2. In race 3 we found no disequilibrium with any of the two methods. Method 3 indicated that several allele pairs were significantly associated in race 2 but not race 3. Thus GPD was significant in race 2 but not in race 3. Mating type frequencies were close to 0.50 in race 2 and race 3. This and the non-significant index of association indicate that the sexual stage ofC. carbonum is operating within each of the two race populations. It is suggested that selection favours different haplotypes in the asexual than in the sexual stage of the pathogen. Such disruptive selection may have maintained polymorphisms of genes related to sexual fertility and stabilized gene frequencies inC. carbonum over a period of 15 years (1972–1987).
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  • 98
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    Plant and soil 162 (1994), S. 309-313 
    ISSN: 1573-5036
    Keywords: in vitro translation ; maize ; nutrient stress proteins ; Poly(A)+RNA ; sulphate deprivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Roots of ten-days-old seedlings obtained from a maize hybrid grown in complete or in sulphate-deprived medium were used to extract Poly(A)+RNA. The response to sulphate deprivation, which is known to increase the uptake capacity up to ten times, was manifested also by the expression of three mRNA species, as shown by the in vitro translation of the mRNA population. One hour after transfer to complete nutrient medium all three mRNAs were still present.
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  • 99
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    Plant and soil 165 (1994), S. 327-332 
    ISSN: 1573-5036
    Keywords: gravitropism ; maize ; nodal roots ; plagiogravitropism ; seminal roots ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The direction of root growth can be studied by analyzing the trajectories of roots growing in soil. Both the primary seminal root and nodal roots of maize attain a preferred, or liminal, angle of growth that deviates from the vertical. These roots are said to be plagiogravitropic. Experiments using plants grown in soil-filled boxes revealed that the primary seminal root is truly plagiogravitropic. It shows both positive and negative gravitropism in response to gravity stimuli and tends to maintain its direction even after growing around obstacles. These are experimental results suggesting that plagiogravitropic growth is controlled by internal factors. The orientation of the grain affects the establishment of the liminal angle of the primary seminal root, and both the position of their node of origin and the root diameter are closely related to the plagiogravitropic behaviour of nodal roots. Several external factors are also known to influence plagiogravitropism. Low soil water content causes a decrease in the angle of growth and soil mechanical resistance suppresses the gravitropic curvature. Plagiogravitropic behaviour of both seminal and nodal roots plays a significant role in shaping the root system.
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  • 100
    ISSN: 1573-5060
    Keywords: abscisic acid ; inheritance ; drought stress ; Zea mays ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study analyzes the components of phenotypic variation for abscisic acid (ABA) content in maize (Zea mays L.) leaves and the correlations with drought sensitivity index (DSI) and silk delay (SD), involved in the reaction to water deficit. Eight early- and seven medium-maturity inbreds were examined in field trials: in 1990 with low irrigation volume and in 1991 with low and high irrigation volumes. ABA concentration and DSI were investigated at growth stages (S) corresponding to stem elongation (S3), appearance of the first husks (S4), and mid-end of silking (S5). The ABA concentration was significantly higher in conditions of water deficit and in the later growth stage. The genetic component for ABA concentration attained higher relative values than those shown by DSI in the same growth stages and by SD; moreover, it increased from growth stage 3 to stage 5. The genotype × year and genotype × irrigation volume interactions were smaller for ABA concentration than for DSI and SD. The broad sense heritability on a plant basis, estimated in drought conditions, for ABA concentration ranged from 21.4 to 55.1% according to maturity group and growth stage. A wide variation was observed among lines for ABA concentration: the medium-maturity group showed a three-fold range (from 219 to 605 ng ABA g−1 dry weight). No clear relationships between ABA concentration, DSI and SD were found. These results indicate the feasibility of a selection for ABA concentration within segregating populations derived from crosses between the inbred lines herein tested.
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