ISSN:
1435-0661
Source:
Springer Online Journal Archives 1860-2000
Topics:
Geosciences
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
4 NO3, ranging from 0 to 120 kg N ha−1. In addition, the effect of soil sieving and storage temperature and time on amino acid mineralization was also investigated. At times ranging from 1 to 40 d after the addition of the inorganic N, the mineralization kinetics of an equimolar mixture of fifteen 14C-labeled amino acids was followed for a subsequent 24-h period. The rate of 14CO2 evolution was soil dependent, with half-lives ranging from 2 h for topsoils to 25 h for subsoils. For all soils, at all times, and at all inorganic-N loadings, the addition of inorganic N appeared to have little effect on the mineralization kinetics of the amino acids to 14CO2. In addition, the presence of inorganic N also had no major effect on the C use efficiency of the microbial biomass. It is speculated that N release from the amino acids into the soil by the microbial biomass may also be little affected by inorganic-N additions. Sieving and storage of soil at either 4 or 18°C for up to 40 d had little impact on amino acid mineralization rate. Experiments with potential microbial disrupting agents (autoclaving, CHCl3 fumigation, HgCl2, and freeze–thaw) all indicated that the observed mineralization of amino acid C was due to microbial activity. We conclude therefore that inorganic N and soil storage has little effect on the microbial use of readily assimilatable amino acids.
Type of Medium:
Electronic Resource
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