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  • Articles  (130)
  • nitrate  (69)
  • 15N  (64)
  • 1990-1994  (130)
  • 1960-1964
  • 1950-1954
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (130)
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  • Articles  (130)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 18 (1994), S. 231-236 
    ISSN: 1432-0789
    Keywords: A-value ; Bradyrhizobium ; Genotype ; Growth stage ; 15N ; Nitrogen fixation ; Nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract TheA-value method, involving the application of a higher15N rate to a reference non-N2-fixing plant, was used to assess the magnitude of N2 fixation in two bambara groundnut cultivars at four growth stages [vegetative, 0–47 days after planting (DAP); early pod-filling, 47–99 DAP; mid-pod-filling, 99–120 DAP; physiological maturity, 120–148 DAP). The cultivars were Ex-Ada, a bunchy type, and CS-88-11, a slightly spreading type. They were grown on a loamy sand. Uninoculated Ex-Ada and CS-88-11 were used as reference plants to measure the N2 fixed in the inoculated bambara groundnuts. In this greenhouse study, soil was the major source of N in bambara groundnuts during vegetative growth, and during this period it accounted for over 80% of the N accumulaed in the plants. However, N2 fixation became the major source of plant N during reproductive growth. There were significant differences between the two cultivars in the ability to fix N2, and at physiological maturity, almost 75% of the N in CS-88-11 was derived from the atmosphere compared to 55% in Ex-Ada. Also, the total N fixed in CS-88-11 at physiological maturity was almost double that in Ex-Ada. Our data indicate that the higher N2 fixation in CS-88-11 was due to two factors, a higher intensity of N2 fixation and a longer active period of N2 fixation. The results also suggest that bambara groundnut genotypes could be selected for higher N2 fixation in farining systems.
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  • 2
    ISSN: 1573-0867
    Keywords: 15N ; residual N ; Sesbania ; urea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field studies were conducted during two consecutive wet seasons in flooded rice (Oryza sativa L.) to determine the effect of green manure on urea utilization in a rice-fallow-rice cropping sequence. Replicated plots were fertilized with 60 to 120 kg of urea N ha−1 in three split applications (50, 25 and 25%) with or without incorporation of dhaincha (Sesbania aculeata L.) (100 kg N ha−1). During the first crop only 31 to 44% of the urea added was used by the rice. Incorporatingin situ grown dhaincha (GM) into the soil at transplanting had little effect on urea utilization. Forty-four to 54% of the N added was not recovered in the soil, rice crop, or as nitrate leachate during the first cropping season. Incorporation of GM had no effect on fertilizer N recovery. Only about 2% of the urea N added to the first rice crop was taken up by the second rice crop and, as in the first crop, the GM had little effect on residual N, either in amount or utilization.
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  • 3
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    Springer
    Nutrient cycling in agroecosystems 37 (1994), S. 159-163 
    ISSN: 1573-0867
    Keywords: leaching ; nitrate ; underground water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of nitrogen fertilizer application on nitrate leaching and contamination of underground and surface waters in a continuously cropped lowland area of South Western Nigeria has indicated a high potential for nitrate leaching. It was estimated that with 100 kg N ha−1 applied, as much as 29.5 kg N ha−1 could be lost through leaching below the root zone of a maize crop, Over a 3 year period the applied nitrogen contributed to nitrate pollution of underground water significantly in excess of the maximum level accepted for potable water. This was particularly high in valley bottoms where the nitrate nitrogen content ranged from 12.8 to 24.6 mg L−1. Contribution to adjacent stream was, however, not significant.
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  • 4
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    Springer
    Biology and fertility of soils 17 (1994), S. 101-107 
    ISSN: 1432-0789
    Keywords: Nutrient cycle ; Coniferous forest ; Soil properties ; 15N ; Acidification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The fate of inorganic 15N added to different coniferous forest soils was traced throughout the soil profile (0–25 cm) in a laboratory experiment under controlled conditions of temperature and water content. Six soils with different chemical climates were compared. The sequestration of labelled N was significantly explained by the clay content but the correlation was improved when C and N content were included. The level of acidification, even in soil with a fine texture, reduced the immobilization. For a similar N input, sandy soils with low C content or high acidification showed a reduced N storage capacity, so that N excess would be able to pollute the ground-water.
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  • 5
    Electronic Resource
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    Springer
    Biology and fertility of soils 17 (1994), S. 32-38 
    ISSN: 1432-0789
    Keywords: Organic matter ; Extracellular enzymes ; Solubilization ; Mineral N ; Total soluble N ; 15N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a sandy soil containing 15N-labeled active (soluble and easily degradable) and non-labelled passive (recalcitrant) fractions of soil organic matter, the rate of net N mineralization (solubilization) was determined during a 55-day incubation at 25°C, 63% water-holding capacity and different levels of soil extracellular-enzyme activities. The active fraction of soil N was labelled by preincubation (at 5°C and 74% water-holding capacity for 6 months) of soil amended with 15N-labeled plant material. Increases in the activity of extracellular-enzymes in soil were induced by the addition of glucose and KH2PO4 at the beginning of the incubation. The results show that the contents of total soluble N (NO 3 − −N+NH 4 + −N + soluble organic N) were significantly higher in glucose-amended soil compared to the unamended soil. The increases in soluble N in soil amended with 1 and 2 mg glucose g-1 dry soil corresponded to a mean rate of net solubilization of 7.9±1.4 and 18.8±0.7 nmol N g-1 dry soil day-1, respectively. The mean rate of net N solubilization (3.6±1.0 nmol N g-1 dry soil day-1) in unamended soil was significantly lower than those of glucose amended soils. The content of 15N in total soluble N in soil amended with 2 mg glucose, for example, was diluted from 3.11±0.08 atom% before the incubation to 2.77±0.03 atom% after 55 days. This indicates that 89% of soluble-N accumulated in soil by the end of the incubation originated from the active fraction of soil N and the rest, estimated at 11%, originated from the passive fraction. The activities of soluble and total proteases as well as the rate of N solubilization in the soil increased with the application of glucose. The activity of these extracellular enzymes was highly correlated with the rates of net N solubilization. Thus, increases in extracellular-enzyme activities in glucose-amended soils had a priming effect on the solubilization of 15N-labeled active and non-labeled passive fractions of soil organic N. It seems that the activity of extracellular-enzymes expressed in terms of total and soluble protease activities could be a rate-limiting factor in the processes of soil organic N solubilization.
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  • 6
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; dry matter partition ; nitrogen content ; DW/Nratio ; nitrate ; ammonium ; glutamic acid ; in vitro nitrogen source
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Different nitrogen sources (NO3 −, NH4 +, glutamic acid and their combinations) influenced the growth and morphogenic responses (node number, shoot length, and stem, leaf and root dry weight) of three micropropagated potato cultivars (Spunta, Kennebec, Huinkul). Addition of reduced nitrogen (NH4 + or glutamic acid) in a nitrate medium increased shoot length and leaf number. The large increase in growth in plants fed with NO3 −, NH4 + could be explained by higher organic nitrogen content and enhanced dry matter partition to the shoot. This suggests that reduced nitrogen source is required, at least as a supplement to NO3 −, to enhance N assimilation and growth.
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  • 7
    ISSN: 1573-5036
    Keywords: alfalfa ; bi-directional N transfer ; bromegrass ; long-term N transfer ; short-term N transfer ; 15N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Transfer of N from legumes to associated non-legumes has been demonstrated under a wide range of conditions. Because legumes are able to derive their N requirements from N2 fixation, legumes can serve, through the transfer of N, as a source of N for accompanying non-legumes. Studies, therefore, are often limited to the transfer of N from the legume to the non-legume. However, legumes preferentially rely on available soil N as their source of N. To determine whether N can be transferred from a non-legume to a legume, two greenhouse experiments were conducted. In the short-term N-transfer experiment, a portion of the foliage of meadow bromegrass (Bromus riparius Rhem.) or alfalfa (Medicago sativa L.) was immersed in a highly labelled 15N-solution and following a 64 h incubation, the roots and leaves of the associated alfalfa and bromegrass were analyzed for 15N. In the long-term N transfer experiment, alfalfa and bromegrass were grown in an 15N-labelled nutrient solution and transplanted in pots with unlabelled bromegrass and alfalfa plants. Plants were harvested at 50 and 79 d after transplanting and analyzed for 15N content. Whether alfalfa or bromegrass were the donor plants in the short-term experiment, roots and leaves of all neighbouring alfalfa and bromegrass plants were enriched with 15N. Similarly, when alfalfa or bromegrass was labelled in the long-term experiment, the roots and shoots of neighbouring alfalfa and bromegrass plants became enriched with 15N. These two studies conclusively show that within a short period of time, N is transferred from both the N2-fixing legume to the associated non-legume and also from the non-legume to the N2-fixing legume. The occurrence of a bi-directional N transfer between N2-fixing and non-N2-fixing plants should be taken into consideration when the intensity of N cycling and the directional flow of N in pastures and natural ecosystems are investigated.
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  • 8
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    Plant and soil 162 (1994), S. 113-116 
    ISSN: 1573-5036
    Keywords: ammonium ; nitrate ; split-roots ; root exudate ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of plant-root N-status on bacterial growth in the rhizosphere was studied with 5-week-old wheat plants grown in soil with low N content obtained by mixing 9:1 gravel:sandy loam. As a consequence of N limitation, significant increase in3Hthymidine (Tdr) incorporation rate occured 3 days after addition of 30 mM ammonium compared to controls without ammonium. Plants were grown with split-roots to separate the effect of soil N from effect of plant root derived organic matter-N on bacterial activity. The increase in nitrate concentration from 10 mM to 30 mM at one part of the root system led to significant increased3HT dr incorporation in the rhizosphere at the other part of root system after 4 days showing that the composition of root exudates became more favourable for bacterial growth when plants were fertilized with the higher level of nitrate.
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  • 9
    ISSN: 1573-5036
    Keywords: nitrate ; nitrate leaching ; nitrogen availability ; nitrogen uptake ; plant indicator method ; spring wheat ; Triticum aestivum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A long-term comparison between two routine soil nitrogen tests, soil nitrate versus plant indicator method, was performed on the Negev Desert loessial soil in Israel. The Gilat plant indicator method was found to be a better method to reflect the soil nitrogen availability for wheat under field conditions. It was found that 15 to 38 kg ha-1 of NO3-N, measured by nitrate soil test, for each 30 cm soil increment, is not available for plant uptake. This plant unavailable NO3-N background in the soil cannot be leached by repeated irrigation cycles of 100 mm each, or by heavy rains.
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  • 10
    ISSN: 1573-5036
    Keywords: ammonium nitrate ; green manure ; incorporation of plant material ; 15N ; N loss ; N mineralization ; NO emission ; oilseed rape ; organic N pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was carried out at a pilot plot that was cropped with oilseed rape, and then left partly fallow and partly cropped with a green manure (mustard) during the autumn after harvest of the oilseed rape. The rape residues were incorporated in the soil. Methods used to quantify the N fluxes from harvest until sowing of the next crop were (1) 15N balance method, (2) total mineral N analysis and (3) NO emission measurements. Losses of spring applied fertilizer N were negligible in cropped plots and minimal in fallow plots during the following autumn-winter period. Most of the plant-N residues was retained by the organic N pool of the upper 30-cm soil layer. The green manure contributed slightly to soil available N at sowing of the next crop. However, the incorporation of plant material resulted in a nitrate flux that was at risk of leaching on the fallow plots, and on the green manure plots after incorporation of the green manure. This nitrate was largely derived from soil organic N, not from unused fertilizer applied in spring or from immobilized fertilizer. The NO emissions from the green manure plots were significantly higher than emissions from the fallow plots. The plants had a stimulating effect on the NO emission. A relationship between the NO emission and the soil nitrate concentration could not be established. No emissions were measured after green manure incorporation due to the low temperatures at the pilot plot. However, a greenhouse experiment showed an increased emission after incorporation. The NO emissions seemed to be related with the soil ammonium concentration.
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  • 11
    ISSN: 1573-5036
    Keywords: mobile N ; N transfer ; 15N ; root damage ; stored N ; white clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An experiment is described in which the magnitude of N transferred from damaged white clover roots to perennial ryegrass was determined, using 15N labelling of the grass plant. There was no effect on the growth and N-fixation of the clover plants after removing part of the root system. The 15N data suggested that N had been acquired by all grass plants, even in plants grown alone with no further N supplied after labelling. However, after quantifying the mobile and stored N pools of the grass plants it was evident that significant transfer of N from clover to grass only took place from damaged clover roots. Dilution of the atom% 15N in the roots of the grass plants grown alone, and in association with undamaged clover roots, was explained by remobilisation of N within the plant.
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  • 12
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    Plant and soil 163 (1994), S. 103-109 
    ISSN: 1573-5036
    Keywords: soil mineralogy ; 15N ; clay fixed ammonium ; fertilizer rate ; fertilizer nitrogen recovery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Clay fixed NH4 + can provide a significant sink for fertilizer N, as well as a source of N for plant uptake. Knowledge or soil NH4 + fixing capacity and release for crops is necessary to develop long-term fertilizer programs. Field experiments with corn (Zea mays L.) were carried out to investigate soil NH4 + fixing capacity and subsequent release as influenced by fertilizer rates using 15N in a Ste. Rosalie clay (fine, mixed, frigid, Typic Humaquept) and a Chicot sandy clay loam (fine-loamy, mixed, frigid, Typic Hapludalf). With high N rates increased NH4 + fixation occurred only in the Ste. Rosalie soil. At the end of the first growing season, fertilizer N recovery as clay fixed NH4 + for high and normal rates of fertilizer in the Ste. Rosalie soil was 17.8% and 28.7%, respectively and the recovery for the high and normal rates in the Chicot soil was 4.6 and 10.5%, respectively. Significant amounts of clay fixed NH4 +-N were released in the soil profile in the second year after 15N application on the Chicot soil. Recently clay fixed fertilizer NH4 +N was released more rapidly than that of the native fixed NH4 +, from the surface layer of the Ste. Rosalie soil. The fertilizer fixed NH4 + seems to be in a more labile N pool than the native fixed NH4 +-N in the Chicot soil.
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  • 13
    ISSN: 1573-5036
    Keywords: basal stem ; nitrate ; Rhizoctonia solani ; root disease ; spring wheat ; tissue nutrient concentrations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Root disease caused by Rhizoctonia solani is a common problem of spring wheat in South Australia. There are reports that nitrogen applications can reduce the incidence and severity of the disease. A glasshouse trail in pots examined the effects of disease and of applied nitrogen on wheat growth, and evaluated the utility of the basal stem nitrate concentration in diagnosing deficiency in plants with and without root disease. Plants were harvested at the mid-tillering stage. Shoot growth was increased by applied nitrogen until a maximum yield was attained, after which additional N had no effect on shoot yield. Root growth, however, responded positively only to low levels of applied N, after which it declined, and in the highest N treatment root mass was less than in the plants without applied N. Root disease caused severe reductions in plant growth, and both root and shoot mass were affected similarly. Even though growth of diseased plants responded positively to applied nitrogen the response was less than that of disease-free plants. The critical concentration of basal stem nitrate-N did not appear to be affected by root disease, and was estimated at 1200 mg kg-1, consistent with other glasshouse data. The basal stem nitrate-N concentration, either in fresh or dried tissue, appeared a better diagnostic tool of N stress than did total shoot N concentration or content, because of sharper definition of critical concentrations. Concentrations of other nutrients in shoot tissue were affected differentially by both applied nitrogen and root disease, but generally did not reach critical levels, although phosphorus and magnesium appeared deficient in very disease-stressed plants.
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  • 14
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    Plant and soil 166 (1994), S. 137-152 
    ISSN: 1573-5036
    Keywords: mineralization ; nitrate ; nitrogen model ; rye grass ; soil nitrogen pools ; spring cereals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of an undersown catch crop on the dynamics and leaching of nitrogen in cropping systems with spring cereals were investigated in southern Sweden. Field measurements of soil mineral nitrogen and nitrogen concentrations in drainage water were made for 4 years in a sandy soil. The experiment was performed on four tile-drained field plots sown with spring cereals. On two of the plots, Italian rye grass was undersown and ploughed down the following spring during three of the years. The other two plots were treated in a conventional way and served as controls. Soil nitrate levels were substantially reduced in the catch-crop treatment, but increased during the fourth year when no catch crop was grown. The differences between the treatments in soil nitrate were reflected in the nitrate concentrations measured in the drainage water. A mathematical model was used to simulate nitrogen dynamics in corresponding treatments. There was good agreement between measurements and simulations with regard to patterns of change in soil mineral nitrogen and nitrate concentrations in drainage water for each treatment. Simulated leaching of nitrate in the conventional treatment was 1.9–3.9 g N m−2 y−1 during the first three years while calculated leaching based on the measurements was 2.7–4.4 g N m−2 y−1. In the catch-crop treatment leaching of nitrate was reduced by 1.4–2.6 g m−2 y−1 according to the simulations and by 2.2–4.1 g m−2 y−1 according to calculations based on the measurements. Measurements showed that leaching of nitrogen compounds other than nitrate was hardly affected by the catch crop. In the simulations the ploughed-down catch crop resulted in temporary increases of the litter pool, a net increase of the humus pool and a reduced C-N ratio of the litter pool. Simulated net mineralization from the litter pool was substantially higher in the catch-crop treatment compared with the conventional treatment. In the fourth year, the yield of the main crop was 20–25% higher in the catch-crop treatment, and leaching was higher than in the conventional treatment.
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  • 15
    ISSN: 1573-5036
    Keywords: ammonium ; denitrification ; cattle slurry ; grassland ; nitrate ; nitrous oxide ; peat soils ; slurry application ; soil conditions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The impact of cattle slurry on denitrification losses and nitrous oxide emission was determined on a peat soil in the Netherlands. As well as measuring losses on a day-to-day basis after three different methods of slurry application, two methods for estimating denitrification and nitrous oxide emissions were compared, i.e. coring/incubation and enclosure techniques. Slurry was applied either in a conventional way, diluted 1:3 or acidified with nitric acid. There was much variation with time, method of assessment and method of slurry application in both apparent denitrification rates and N2O fluxes: it was not always possible to provide adequate explanation for all of the effects demonstrated. Major proportions of the variation in denitrification and N2O emission could be accounted for by variation in soil moisture, soil temperature and NH4 + (but not NO3 -) content. It was suggested that nitrification was playing a key role in maintaining an adequate substrate supply (NO3 -) for denitrification and perhaps contributing directly to an unknown extent to N2O emissions. There were overall differences in the extent of losses with the different methods of slurry application but these were highly dependent upon interactions with current soil and weather conditions.
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  • 16
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    Plant and soil 162 (1994), S. 249-257 
    ISSN: 1573-5036
    Keywords: lettuce ; maximum inflow (Imax) ; model ; nitrate ; relative growth rate ; root:shoot-ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The net inflow of nitrate can be calculated from the nitrate concentration at the root surface by means of the Michaelis-Menten equation. Because of maximum inflow (Imax) is not constant but varies with plant age and growing conditions, a model for calculating Imax during plant growth was derived. Lettuce was grown in nutrient solution. Variations in temperature, radiation and plant age were used to vary growth rates and N-demand of plants. There was a linear relationship between relative growth rates (RGR) and maximum nitrate inflow (Imax), that could be described by the following regression function: Imax = 0.24 + 6.57 RGR. A residual analysis showed a further influence on Imax from the root:shoot-ratio (RSR), the effects of which could be accounted for by including an e-function in the relationship: Imax = (0.27 + 10.63 RGR) e(−0.0017 RSR). This model for calculating Imax was validated in two further experiments.
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  • 17
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    Plant and soil 163 (1994), S. 267-277 
    ISSN: 1573-5036
    Keywords: cultivar ; critical root length density ; field experiment ; nitrate ; N utilization ; root growth ; uptake rate ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a 2-year field study conducted on a high fertilized Gleyic Luvisol in Stuttgart-Hohenheim significant differences among 10 maize cultivars were observed in soil nitrate depletion. The different capability of the cultivars to utilize nitrate particularly from the subsoil was positively correlated with (a) shoot N uptake at maturity, and (b) root length density (Lv) in the subsoil layers at silking. “Critical root length densities” for nitrate uptake were estimated by (a) calculating uptake rates per unit root length (U), (b) subsequent calculation of needed nitrate concentration in soil solution (C1) to sustain calculated U according to the Baldwin formula, and (c) reducing measured Lv and proportionate increase of U until needed concentration equaled measured concentration. Uptake rate generally increased with soil depth. “Critical root length densities” for cultivar Brummi (high measured root length densities and soil nitrate depletion) at 60–90 cm depth ranged from 7 % (generative growth) to 28 % (vegetative growth) of measured Lv Measured root length density of each other cultivar was higher than “critical root length density” for Brummi indicating that the root system of each cultivar examined would have been able to ensure N uptake of Brummi. Positive relationships between root length density and nitrate utilization as indicated by correlation analysis therefore could not be explained by model calculations. This might be due to simplifying assumptions made in the model, which are in contrast to non-ideal uptake conditions in the field, namely irregular distribution of roots and nitrate in the soil, limited root/soil contact, and differences between root zones in uptake activity. It is concluded from the field experiment that growing of cultivars selected for high N uptake-capacity of the shoots combined with “high” root length densities in the subsoil may improve the utilization of a high soil nitrate supply.
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  • 18
    ISSN: 1573-5036
    Keywords: Al avoidance ; Al tolerance ; ammonium ; Mucuna ; nitrate ; split-root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract InMucuna pruriens var.utilis, grown with nitrate-N in a hydroponic split-root system, an Al avoidance reaction of root growth was observed, which was ascribed to local P stress in the Al containing compartment. The Al avoidance reaction was similar to the avoidance ofMucuna roots of acid subsoil in the field where roots grew preferentially in the topsoil. In the present paper the effect of different N forms (NO3 − and NH4 +) on the reactions ofMucuna to Al were studied, since in acid soils N is present as a mixture of NO3 − and NH4 +. No interaction between the N form and Al toxicity was found. A hydroponic split-root experiment with NH4NO3 nutrition, which is comparable to the situation in the field, showed that under these conditions Al avoidance did not occur. It is concluded that a relation between the Al avoidance reaction ofMucuna and P stress is still likely.
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  • 19
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    Nutrient cycling in agroecosystems 37 (1994), S. 93-105 
    ISSN: 1573-0867
    Keywords: Erosion/Productivity Impact Calculator (EPIC) model ; leaching ; lettuce ; net N mineralization ; nitrate ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Low N use efficiency and high nitrate (NO 3 - ) pollution potentials are problems in intensive vegetable production systems. The purpose of this study was to quantify N utilization by lettuce (Lactuca sativa L. cv Salinas), and identify periods of NO 3 - loss in an on-farm study in the Salinas Valley in coastal California. During autumn and winter, surface moisture remained low, and NO 3 - concentrations increased, reflecting high net mineralizable N, as determined by anaerobic incubation, and nitrification potential, as determined by the chlorate inhibition method. At the onset of a large winter storm, tracer levels of15NO 3 - were injected in the top 5 mm of soil in 30 cm-deep cylinders. After two weeks, most of the15N was present as15NO 3 - at 10–30 cm depth. By difference, losses to denitrification accounted for ~ 25% of the surface-applied15N. Leaching below 30 cm did not occur, since no15N enrichment of NO 3 - -N was measured in anion-exchange resin membranes placed at the base of each cylinder. During the crop period, NO 3 - losses were most pronounced after irrigation events. Uptake of N by two crops of lettuce (above- and belowground material) was approximately equal to fertilizer inputs, yet simulation of N fates by the Erosion/Productivity Impact Calculator (EPIC) model indicated losses of 14.6 g-N m−2 by leaching and 2.5 g-N m−2 by denitrification during the 6-month crop period. The large NO 3 - losses can be attributed to accumulation of soil NO 3 - during winter that was leached or denitrified during the irrigated crop period.
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  • 20
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    Nutrient cycling in agroecosystems 38 (1994), S. 239-247 
    ISSN: 1573-0867
    Keywords: catch crops ; immobilization ; nitrate ; soil watrer tension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A pot incubation method for measuring mineralization dynamics from fresh plant material was tested. The aim was to develop a method which under well-defined conditions could produce mineralization data suited for estimating model input parameters for nitrogen prediction models. The results showed that the water tension of the soil could be controlled easily and precisely by diffusion through porous ceramic cups, and that nitrogen mineralization or immobilization could be measured already after 15 days at 15°C. The results showed that for the incubated catch crop residues carbon, nitrogen and nitrate-N contents were the most important factors determining mineralization. No significant effects ould be ascribed to other parameters measured.
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  • 21
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    Nutrient cycling in agroecosystems 38 (1994), S. 131-139 
    ISSN: 1573-0867
    Keywords: aqua ammonia ; di-ammonium phosphate ; gamma-irradiated soil ; 15N ; organic matter solubility ; urea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Interactions between15N-labelled fertilizers applied at concentrations representative of the fertilizer microsite and the solubility of the nitrogenous component of soil organic matter were investigated in laboratory experiments. Soil organic N was solubilized in aγ-irradiated soil due to addition of NH3(aq), and the fertilizer-induced loss of unlabelled total N in the extracted soil (ΔTUs) increased with increasing N fertilizer concentration and soil pH. ΔTUs was linearly correlated with ammoniacal-N concentration and the pH of the fertilized soil within the range of 7.5-10 (r = 0.94). Total organic N in the soil extract (OTe) increased rapidly up to day 14 following addition of 2000 mg urea-N kg−1 soil, but was then stable up to day 28. OTe of a range of soils increased from between 5 and 148 to between 15 and 368 mg N kg−1 soil after application of 1045 mg NH3-N kg−1 soil. While up to 25% of the organic N was solubilized by the fertilizer in nine soils, the change in total organic N in the extracts (ΔOTe) of three soils was not significant. The highest ΔOTe of 399 mg N kg−1 soil (35.4% of soil organic N) was measured after application of 2000 mg NH3-N kg−1 soil. pH and ΔOTe decreased in the order of NH3(aq) 〉 urea 〉 di-ammonium phosphate 〉 ammonium sulphate at equivalent rates of N addition. A negative ΔOTe was measured following application of ammonium sulphate. ΔOTe was correlated with the pH of the fertilized soil but not ammoniacal-N concentration for different N fertilizer sources.
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  • 22
    ISSN: 1573-5036
    Keywords: ammonium ; arctic plants ; nitrate reductase ; nitrogen uptake ; 15N ; root temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We investigated whether six arctic plant species have the potential to induce nitrate reductase (NR) activity when exposed to NO3 --nitrogen under controlled environment conditions, using an in vivo assay that uses the rate of NO2 --accumulation to estimate potential NR activity. We also assessed the effect of low root temperatures on NR activity, growth and nitrogen uptake (using 15N applications) in two of the selected species. Five of the six species (Cerastium alpinum, Dryas intergrifolia, Oxyria digyna, Saxifraga cernua and Salix arctica) were capable of inducing NR activity when exposed to solutions containing 0.5 mM NO3 - at 20°C for 10 days. Although in vivo NR activity was not induced in Saxifraga oppositifolia under controlled conditions, we conclude that it was capable of growing successfully on NO3 -, due to the presence of moderate rates of NR activity observed in both NH4 +-grown and NO3 --treated plants. Exposure of O. digyna and D. integrifolia to 3°C root temperatures for two weeks, with the shoots kept at 20°C, resulted in root and leaf NR activity rates of NO3 --treated plants being reduced to rates exhibited by NH4 +-grown plants. Although these decreases in NR in both species appeared to be due to limitations in NO3 --uptake and growth rate (rather than direct low-temperature inhibition of NR synthesis per se), direct low-temperature inhibition of root NR synthesis could not be ruled out. In contrast to the temperature insensitivity of NH4 + uptake in D. integrifolia, NO3 --uptake in D. integrifolia was inhibited by low root temperatures. We conclude that the selected arctic species have the genetic potential to utilize NO3 --nitrogen, and that low root temperatures, in conjunction with other environmental limitations, may be responsible for the lack of induction of NR in D. integrifolia and Salix arctica under field conditions.
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  • 23
    ISSN: 1573-5036
    Keywords: 15N ; Cucumis sativus ; Glomus intraradices ; hyphal N transport ; plant N status ; VA mycorrhiza
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cucumis sativus L. cv. Aminex (F1 hybrid) was grown alone or in symbiosis with Glomus intraradices Schenck and Smith in containers with two hyphal compartments (HCA and HCB) on either side of a root compartment (RC) separated by fine nylon mesh. Plants received a total of either 100, 200 or 400 mg N which were applied gradually to the RC during the experiment. 15N was supplied to HCA 42 d after plating, at 50 mg 15NH4 +-N kg−1 soil. Lateral movement of the applied 15N towards the roots was minimized by using a nitrification inhibitor and a hyphal buffer compartment. Non-mycorrhizal controls contained only traces of 15N after a 27 d labelling period irrespective of the amount of N supplied to the RC. In contrast, 49, 48 and 27% of the applied 15N was recovered in mycorrhizal plants supplied with 100, 200 and 400 mg N, respectively. The plant dry weight was increased by mycorrhizal colonization at all three levels of N supply, but this effect was strongest in plants of low N status. The results indicated that this increase was due partly to the improved inflow of N via the external hyphae. Root colonization by G. intraradices was unaffected by the amount of N supplied to the RC, while hyphal length increased in HCA compared to HCB. Although a considerable 15N content was detected in mycorrhizal roots adjacent to HCB, only insignificant amounts of 15N were found in the external hyphae in HCB. The external hyphae depleted the soil of inorganic N in both HCA and HCB, while the concentration of soil mineral N was still high in non-mycorrhizal containers at harvest. An exception was plants supplied with 400 mg N, where some inorganic N was present at 5 cm distance from the RC in HCA. The possibility of a regulation mechanism for hyphal transport of N is discussed.
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  • 24
    ISSN: 1573-5036
    Keywords: compensatory root growth ; localized nutrient supply ; nitrate ; phosphate ; potassium ; split-pot system ; vermiculitic soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Vertical stratification of plant-available K in vermiculitic soil profiles contributes to a late-season K deficiency that limits cotton (Gossypium hirsutum L.) yields on affected soils. Split-root solution culture and split-pot soil experiments were conducted to determine whether root distribution and cultivar differences in root extension in these stratified profiles result from a compensatory response to localized enrichment with NO3-N, PO4-P, and/or K in the root zone. Compensatory root growth was greatest in response to localized NO3-N enrichment. For two cultivars examined in solution culture, 74% of new root development occurred in the half-pot providing 90% of the total NO3-N supply. Only 60% of cultivar root development occurred in the half-pot providing 90% of the PO4-P. No compensatory root growth was observed in response to localized K enrichment. In the split-pot system, the proportion of total root surface area developing in a half-pot was highly correlated with localized soil NO3-N levels (r2=0.81), while increased K availability in one half of the root zone did not affect root distribution. Mean soil NO3-N supply to the whole root system determined shoot N accumulation (r2=0.97). Shoot K accumulation was not related to soil K availability but was strongly correlated with mean root surface area density (r2=0.86). Cultivar ‘Acala GC510’, known to be less sensitive to K deficiency than ‘Acala SJ-2’, had significantly larger root diameter in all nutrient-supply environments. Under conditions of K stress, ‘Acala GC510’ had increased root branching and allocated greater dry matter to roots relative to shoots than ‘Acala SJ-2’. The results demonstrate that K acquisition by cotton is strongly influenced by the quantity and distribution of NO3-N in the root zone through its effects on root proliferation, and that distinct cultivar differences associated with crop performance on low K soils can be detected in short-term, solution culture growth systems.
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  • 25
    ISSN: 1573-5060
    Keywords: Cichorium endiviae ; endive ; cultivars ; quality ; nitrate ; chloride ; osmolarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A high nitrate content is a negative quality trait in vegetables. Endive (Cichorium endiviae L.) contributes considerably to human nitrate intake in Northwestern Europe. A collection of 125 commercially available cultivars of endive were grown on nutrient solution and evaluated for genetic variation between cultivars for the nitrate concentration in expressed sap. In two subsequent experiments 23 cultivars, representing the range of nitrate levels found in the first experiment, were grown both in soil and on nutrient solution. There were significant differences between cultivars in nitrate accumulation. Cultivar ‘Vicor’ consistently had the lowest nitrate content. Averaged over all experiments the nitrate concentration in expressed sap of this cultivar was 0.7 g·l-1 (19%) lower than that of two recommended cultivars. The results indicate that there may be some possibilities to reduce the nitrate content of endive by cultivar choice or breeding. However, the chances of obtaining a substantial reduction of the nitrate content of endive seem less than in some other crops, such as lettuce. The nitrate levels of the cultivars showed interactions with experiments and growth medium. There was no correlation between the cultivar means for the concentrations of nitrate and chloride, or between the concentration of nitrate and the osmolarity of the plant sap.
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  • 26
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    Nutrient cycling in agroecosystems 36 (1993), S. 91-94 
    ISSN: 1573-0867
    Keywords: competing vegetation ; forest disturbance ; nitrate ; nutrients ; succession
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Une fertilisation azotée sous forme de nitrate déclenche la germination de graines deRubus idaeus L. (framboisier sauvage) naturellement enfouies dans le parterre de peuplements forestiers conifériens âgés de 50 ans, situés dans l'est du Québec. Les contrastes orthogonaux ont montré un effet linéaire significatif entre les doses de nitrate appliquées et le dénombrement de semis de framboisier sauvage durant deux saisons de croissance consécutives. Les traitements de fertilisation ont davantage stimulé l'émergence du framboisier sauvage à la seconde année qu'à la première. L'auteur discute brièvement de l'effet possible du nitrate sur la levée de la dormance de graines de framboisier sauvage en relation avec les perturbations forestières.
    Notes: Abstract Nitrate-nitrogen fertilization triggered germination of dormantRubus idaeus L. (red raspberry) seeds naturally buried in the forest floor of 50-year-old coniferous stands of eastern Quebec. Orthogonal contrasts revealed a significant linear effect between the rates of nitrate applied and red raspberry seedling counts over two consecutive growing seasons. The fertilization treatments stimulated emergence to a greater extent in year 2 than in year 1. The potential effect of nitrate on raspberry seed dormancy breakage is briefly discussed in relation to site disturbance.
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  • 27
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    Nutrient cycling in agroecosystems 36 (1993), S. 19-27 
    ISSN: 1573-0867
    Keywords: critical concentrations ; nitrate ; nitrogen fertilization ; petiole ; potato ; short day conditions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments were conducted for three crop seasons to develop tissue analysis technology for optimizing N fertilization in potato grown under short day conditions in subtropics. Nitrogen deficiencies could be detected as early as 25 days after planting (DAP) by tissue analysis of NO3-N concentration in petioles. Petiolar NO3-N declined sharply with age of the crop from 25 to 60 DAP and was significantly correlated at all stages of growth with applied N and tuber yield of potato. Critical concentrations of Petiolar NO3-N were 1.28, 1.23, 1.07 and 0.96% in an early maturing cv. Kufri Chandramukhi and 2.16, 1.95, 1.40 and 1.18% in a late maturing cv. Kufri Badshah at 25, 40, 50 and 60 DAP, respectively. Optimum yields were obtained when petiolar NO3-N was maintained above critical concentrations through the growth period up to 60 DAP by corrective side dressing of N. Response to corrective side dressing of N decreased with increasing concentration of petiolar NO3-N. Optimum rate of N for side dressing up to 30 DAP was 142, 116, 90, 64 and 37 kg ha−1 for petiolar NO3-N of 0.5, 1.0, 1.5, 2.0 and 2.5%, respectively in Kufri Chandramukhi. Corresponding rates of side dressed N were 183, 164, 146, 127, 108 kg ha−1 in late maturing cv. Kufri Badshah. For optimum yields, fertilization of 80 to 120 kg N ha−1 at the time of planting followed by corrective side dressing as recommended by petiolar tissue analysis is advocated.
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  • 28
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    Biology and fertility of soils 15 (1993), S. 225-228 
    ISSN: 1432-0789
    Keywords: Urease inhibitors ; Urea N efficiency ; 15N ; Ryegrass ; Hydroquinone (HQ) ; Phenyl phosphorodiamidate (PPDA) ; N-(n-butyl) phosphorothioic triamide (NBPT)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A greenhouse experiment was conducted to study the comparative efficiency of urea as an N fertilizer with and without the addition of different urease inhibitors. Ryegrass (Lolium perenne L.) was used as the test plant and the N balance technique with 15N was applied. Three urease inhibitors, hydroquinone, phenyl phosphorodiamidate (PPDA), and N-(n-butyl) phosphorothioic triamide (NBPT), were evaluated for their effects on urea-N uptake as well as on grass yield. The addition of urease inhibitors, except for hydroquinone in the later growth period, did not significantly influence the dry matter weight. Throughout the whole growth period, only NBPT significantly increased the total urea-N uptake. In the uninhibited system, the major fertilizer N loss occurred during the first period of grass growth, presumably via NH3 volatilization, since the environment did not favour the other pathways of N loss. However, an appreciable amount of urea N was lost during the later growth period in all inhibited systems, especially in the hydroquinone-treated system. This indicates that the application of urease inhibitors could not eliminate the urea N loss. The greater N loss in the hydroquinone-treated soil appears to be related to the inhibition by hydroquinone of nitrification.
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  • 29
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    Biology and fertility of soils 16 (1993), S. 299-301 
    ISSN: 1432-0789
    Keywords: A N value ; 15N ; Nitrogen fixation ; Glycine max ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pot experiments were conducted with two soils, from Rottenhaus and Seibersdorf in Austria, to ascertain whether the rate of fertilizer N application and the test crop would influence the amount of N available in the soil as assessed by the A-value method. 15N-labelled fertilizer was applied at rates of 10, 25, 40, 60, and 100 mg N kg-1 soil, corresponding approximately to 20, 50, 80, 120 and 200 kg N ha-1 respectively, and two crop species, barley (Hordeum vulgareL.) and non-nodulating soybean (Glycine max L.) were used to determine the soil A N value under the various fertilizer regimes. The results showed that the Rottenhaus soil had a higher A N value than the Seibersdorf soil, suggesting that the former was more fertile than the latter. The A N values of both soils were significantly affected by the level of N application. When grown in the same soil, the two test crops showed significantly different fertilizer use efficiency and per cent N derived from fertilizer when the rate of N application exceeded 20 kg ha-1. Thus, the A N value as determined by the two test crops differed significantly for the same soil when the rate of N application was greater than 20 kg/ha. The difference was greater when the soil fertility level was high. The dependence of the A N value on the level of N application and the species of crop seriously compromises the suitability of this method for determining plant-associated N2 fixation. Hence, considerable caution is required when using this method to estimate plant-associated N2 fixation.
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    Biology and fertility of soils 15 (1993), S. 215-219 
    ISSN: 1432-0789
    Keywords: N2 fixation ; N-supplying ability ; 15N ; Arachis hypogaea ; Intercropping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Identification of legume genotypes with high N-supplying ability is important in improving and sustaining the productivity of low-input cropping systems. Hence, 15N-aided studies were made to ascertain the relative N-supplying ability of some cultivars of groundnut, a widely grown tropical legume. The study was conducted outdoors in 1991 at Kamburupitiya, Sri Lanka, in tanks filled with 64 kg soil which had been tagged by incorporating 15N-labelled plant material. Maize cv. Badra was grown as a monocrop and as an intercrop with five genotypes of groundnut, X-14, MI-1, Red Spanish, ICGV 87127, and a non-nodulating line. All the nodulating genotypes derived over 90% of their N from the atmosphere. Significant genotypic differences in N2 fixation were observed. X-14 fixed the highest amount (1.95 g plant-1), while Red Spanish the lowest (0.88 g plant-1). Intercropping of maize with nodulating groundnut significantly decreased the 15N atom excess of maize, depending on the genotype. However, this decrease did not appear to be related to the amount of N2 fixed, based on aboveground material. The per cent N derived by maize from the intercropped groundnuts varied from 17% (X-14) to 39% (Red Spanish), indicating a marked genotypic variability in N-suppling ability. X-14, which fixed the largest amounts of N2, grew most vigorously compared to other genotypes, causing a growth depression in the maize. The genotype that fixes the most N2 may therefore not necessarily have the greatest N-supplying ability. The transfer of N from the legume and the consequent improvement of N nutrition in the associated cereal in low-fertility situations is therefore expected to be high when the growth of the legume is intermediate and does not suppress the growth of the cereal.
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  • 31
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    Biology and fertility of soils 15 (1993), S. 285-293 
    ISSN: 1432-0789
    Keywords: Manures ; N mineralisation ; N uptake ; 15N ; Added nitrogen interactions ; Priming effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field and laboratory experiments were used to examine the efficiency of N uptake from various manure forms, and at different rates of application. In a field experiment, wheat was grown on soils with different amounts of 15N-labelled legume residues. The amount of N taken up by the crop was directly proportional to the amount applied, with a recovery of between 15% and 23% of the legume N. In a second field experiment, inorganic N was applied at rates varying from 0 to 120 kg N ha-1 in the presence and absence of poultry manure. The uptake of N by barley was 11 kg ha-1 greater in the manured plots when no inorganic N was applied, and 23 kg ha-1 greater when N was applied at the top rate. N uptake in a pot experiment was again shown to be directly proportional to the rate of manure application, but the amount of N taken up was strongly related to the N content of the manure. An incubation experiment demonstrated that net N mineralisation reached a maximum where residue concentrations were 1,5%. The significance of added nitrogen interactions in the context of manure-N additions is discussed.
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  • 32
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    Biology and fertility of soils 16 (1993), S. 125-130 
    ISSN: 1432-0789
    Keywords: N2 fixation ; Ontogeny ; 15N ; Vigna radiata ; Vigna mungo ; Vigna unguiculata ; Arachis hypogaea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ontogenic variations in N2 fixation and accumulation of N by the mungbean (Vigna radiata L. Wilczek), blackgram (Vigna mungo L. Hepper), cowpea (Vigna unguiculata L. Walp.), and groundnut (Arachis hypogaea L.) were studied by a 15N-dilution technique. Pots filled with 7 kg of red yellow podzolic soil were used. Samples were taken 20, 40, 60, and 80 days after emergence which approximately corresponded to preflowering, flowering, early/mid-pod filling and late pod filling stages, respectively. During early growth (up to 40 days after emergence), the carryover of seed N accounted for a considerable fraction of the total plant N in the legumes, the highest being in the groundnut. With a correction for carryover, the groundnut derived over 45% of its N content from the atmosphere 20 days after emergence whereas the corresponding figures were 33% for the blackgram and about 28% for the cowpea and mungbean. Between flowering and early pod fill, there was a rapid increase in N2 fixation in all legumes except in groundnut which showed highest fixation from 60 to 80 days after emergence. In the mungbean, N2 fixation and uptake of soil N were insignificant 60 days after emergence while in other legumes these processes continued beyond this time. All legumes derived about 90% of their N from atmosphere by 80 days after emergence. However, due to considerable interspecific differences in total N yield the final amount of N2 fixed showed an appreciable variation among legumes. It was highest in the groundnut (443 mg N plant-1) followed by the cowpea (385), blackgram (273), and mungbean (145), respectively. The groundnut maintained nodules until the late pod filling stage while in other legumes, nodules senesced progressively following the mid-pod filling stage. During pod filling there was a net mobilization of N from vegetative tissues to developing pods in the mungbean, which amounted to about 20% of N in seeds. This mobilization was not evident in other legumes.
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  • 33
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    Potato research 36 (1993), S. 301-308 
    ISSN: 1871-4528
    Keywords: colour ; petiole ; nitrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A field experiment with potato (Solanum tuberosum L., cv. Vebeca) was conducted on a sandy soil near Wageningen (52° North) in 1992. The treatments included a zero-nitrogen control and combinations of three amounts of nitrogen, viz. 110, 180 and 250 kg N ha−1, and splitting of the N dose in one (early May), two (early May and June) or three (early May, June, July) applications. The chlorophyll content of the uppermost fully grown leaves was assessed with a SPAD-502 chlorophyll meter (Minolta, Osaka, Japan) throughout the season. The pattern of change with time in SPAd-values differed between treatments. SPAD-502 readings correlated well with laboratory measurements of the chlorophyll content and with the nitrogen concentration in leaves (r 2〉0.95). Data on the nitrate concentration in petiole sap (included as a reference) showed that this variable responded much more to split nitrogen applications than the SPAD-value. Future research will need to consider other factors which may affect the chlorophyll content of the foliage.
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  • 34
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    Plant and soil 157 (1993), S. 147-150 
    ISSN: 1573-5036
    Keywords: 15N ; forest soils ; N deposition ; N losses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A direct correlation was found between fractional losses of added N and the change in δ 15N‰ during 19 years in an experiment with annual additions of N at three rates to a Scots pine (Pinus sylvestris L.) forest in northern Sweden. This confirms that processes leading to losses of N discriminate against 15N, and opens possibilities to conduct retrospective studies of the N balance in forests.
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  • 35
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    Plant and soil 151 (1993), S. 97-104 
    ISSN: 1573-5036
    Keywords: aluminium ; ammonium ; induction ; maize ; nitrate ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Experiments with two maize (Zea mays L.) hybrids were conducted to determine (a) if the inhibition of nitrate uptake by aluminium involved a restriction in the induction (synthesis/assemblage) of nitrate transporters, and (b) if the magnitude of the inhibition was affected by the concurrent presence of ambient ammonium. At pH 4.5, the rate of nitrate uptake from 240 μM NH4NO3 was maximally inhibited by 100 μM aluminium, but there was little measurable effect on the rate of ammonium uptake. Presence of ambient aluminium did not eliminate the characteristic induction pattern of nitrate uptake upon first exposure of nitrogen-depleted seedlings to that ion. Removal of ambient aluminium after six hours of induction resulted in recovery within 30 minutes to rates of nitrate uptake that were similar to those of plants induced in absence of aluminium. Addition of aluminium to plants that had been induced in absence of aluminium rapidly restricted the rate of nitrate uptake to the level of plants that had been induced in the presence of aluminium. The data are interpreted as indicating that aluminium inhibited the activity of nitrate transporters to a greater extent than the induction of those transporters. When aluminium was added at initiation of induction, the effect of ambient ammonium on development of the inhibition by aluminium differed between the two hybrids. The responses indicate a complex interaction between the aluminium and ammonium components of high acidity soils in their influence on nitrate uptake. ei]{gnA C}{fnBorstlap}
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  • 36
    ISSN: 1573-5036
    Keywords: cultivar ; nitrate ; nitrate leaching ; N utilization ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a 2-year field experiment conducted on a Gleyic Luvisol in Stuttgart-Hohenheim one experimental and nine commercial maize cultivars were compared for their ability to utilize soil nitrate and to reduce related losses of nitrate through leaching. Soil nitrate was monitored periodically in CaCl2 extracts and in suction cup water. Nitrate concentrations in suction water were generally higher than in CaCl2 extracts. Both methods revealed that all cultivars examined were able to extract nitrate down to a soil depth of at least 120 cm (1988 season) or 150 cm (1987 season). Significant differences among the cultivars existed in nitrate depletion particularly in the subsoil. At harvest, residual nitrate in the upper 150 cm of the profile ranged from 73–110 kg N ha−1 in 1987 and from 59–119 kg N ha−1 in 1988. Residual nitrate was closely correlated with nitrate losses by leaching because water infiltration at 120 cm soil depth started 4 weeks after harvest (1987) or immediately after harvest (1988) and continued until early summer of the following year. The calculated amount of nitrate lost by leaching was strongly influenced by the method of calculation. During the winter of 1987/88 nitrate leaching ranged from 57–84 kg N ha−1 (suction cups) and 40–55 kg N ha−1 (CaCl2 extracts), respectively. The corresponding values for the winter of 1988/89 were 47–79 and 20–39 kg N ha−1, respectively. ei]Section editor: B E Clothier
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  • 37
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    Plant and soil 155-156 (1993), S. 353-354 
    ISSN: 1573-5036
    Keywords: cowpea ; nitrogen fixation ; nitrate ; partitioning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract If the quality and quantity of yields from cowpea (Vigna unguiculata [L.] Walp.) are to be maximised, a complete understanding of the N nutrition of the plant must be achieved. The N requirement for developing pods of this species may come from mobilization of N in vegetative tissue, biological N fixation and uptake of N from soil. In this study, the fate of a pulse of fixed 15N2 or of 15NO3-given to different cowpea plants during pod development was determined. The plants were grown in vermiculite in plastic pots that were able to be sealed with silicone adhesive and equipped with a rubber septum so that 15N2 gas could be injected into the air space above the vermiculite, and gas losses would be eliminated. Nineteen days after injection of 15N2 the pods, leaves, nodules and roots contained 65%, 15%, 9%, and 4%, respectively of the quantity of 15N2 fixed. When 15NO3-15N was taken up by other plants during this period, these plant parts contained 40%, 26%, 3% and 19%, respectively, of the total plant 15N. The percentage 15N in roots was greater, and that of 15N in nodules was lower, when 15NO3-15N was applied than when 15N2 was utilised by plants. These results indicate that, while a high percentage of fixed-N or NO3-N given to cowpea plants moved to the developing pods, other sinks were competing for this newly-aquired N.
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    Plant and soil 157 (1993), S. 147-150 
    ISSN: 1573-5036
    Keywords: 15N ; forest soils ; N deposition ; N losses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A direct correlation was found between fractional losses of added N and the change in δ15N‰ during 19 years in an experiment with annual additions of N at three rates to a Scots pine (Pinus sylvestris L.) forest in northern Sweden. This confirms that processes leading to losses of N discriminate against15N, and opens possibilities to conduct retrospective studies of the N balance in forests.
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    Plant and soil 152 (1993), S. 255-260 
    ISSN: 1573-5036
    Keywords: crimson clover ; field labeling ; legume ; nitrogen ; 15N ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant material labeled with 15N is often used to determine recovery of N from green manure crops by subsequent crops. In this study, 15N enriched crimson clover (Trifolium incarnatum L.) was grown at a field site where it was to be utilized in a subsequent experiment. A foliar spray of (NH4)2SO4 (99 atom % excess 15N) was applied to a 1.2 m × 8.8 m plot of crimson clover at a rate of 10 kg N ha−1 in early March 1990, immediately prior to the period of rapid vegetative growth. Clover shoots harvested in April contained 1.72 atom % excess 15N. Total N concentration of enriched clover was similar to that in adjacent untreated clover. Clover shoots contained 20% of the applied 15N, and an additional 27% was recovered from the surface soil horizon (0 to 15 cm). A gradient was observed across the plot, with clover enrichment increasing from 1.3 to 2.2 atom % excess 15N. Recovery of applied 15N in soil was highest in the subplots with lowest clover enrichment. Variability in 15N enrichment was also observed among plant parts: leaves from the basal half of shoots had 2.2 atom % excess 15N; while leaves from the terminal half of shoots, terminal stems, and basal stems had between 1.1 and 1.4 atom % excess 15N.
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  • 40
    ISSN: 1573-5036
    Keywords: air pollution ; ammonia ; ammonium ; 15N ; N concentration ; needle ; pine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Young saplings of Pinus sylvestris L. were exposed to gaseous NH3 at 53 or 105 μg m−3 for one year in open-top chambers. Saplings received 15N-labelled (NH4)2SO4 via the soil. To examine the importance of foliar N uptake, changes in the concentration of total and labelled N in the needles were followed. Increase in needle biomass and N concentration were found in trees exposed to NH3, confirming that atmospheric NH3 acted as a N fertilizer. NH3 had a greater and quicker effect than (NH4)2SO4: compared with the growth in ambient air, the N concentration in the needles exposed to NH3 had increased by 49% in four months, while the increase after highest N-fertilization (200 kg N ha−1 y−1) was only 8%. The small contribution of NH4 + fertilization to the total N concentration was not due to a deficient N uptake: the 15N concentration in the needles increased significantly with time. On the other hand, NH3 uptake in shoots may have a negative effect on the NH4 + root uptake. The relation between plant N and atmospheric NH3 concentration was non-linear and possible reasons for this observation are discussed. Fumigation with NH3 significantly decreased the ratios of K/N and P/N, showing that fumigation disrupted the nutrient balance.
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  • 41
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    Plant and soil 155-156 (1993), S. 387-390 
    ISSN: 1573-5036
    Keywords: Brassica napus L. ; canola ; critical concentrations ; nitrate ; nitrogen ; sowing time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Critical concentrations of NO3-N in fresh petiole tissue and total N in the dried lamina were determined for the youngest mature leaf (YML) of field-grown canola. For dry matter yield of canola sown on 4 May, critical NO3-N concentration in the YML petiole at the rosette stage (RS) was 1.46 mg/g fresh wt. At the flower-buds-visible stage (BV) it was 0.45 mg/g fresh wt. For seed yield the values were 1.72 and 0.53 mg/g fresh wt. Critical total N concentration in the YML lamina for dry matter yield were 69 mg/g dry wt. at RS and 57 at BV. For seed yield they were 71 and 59 mg/g dry wt. Critical NO3-N concentrations in the YML petiole of canola sown on 30 May were reduced by 50%; critical total-N concentrations in the YML lamina were not reduced to the same extent. Despite the reductions in critical N concentrations in the YML, critical N fertilizer rates for vegetative growth and seed yield were unaffected by sowing date or plant growth stage.
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  • 42
    ISSN: 1573-5036
    Keywords: denitrification ; nitrate ; nitrate reductase ; nitrite ; nitrite reductase ; nitrous oxide evolution ; Rhizobium meliloti
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The denitrifying ability of thirteen strains of Rhizobium meliloti was tested. Most of the strains were able to reduce nitrate to nitrous oxide or dinitrogen. However, they failed to use nitrate as electron acceptor for ATP generation or growth at low oxygen tensions. Under micro-aerobic conditions, free-living cells of R. meliloti 102-F-51 strain exhibited a constitutive nitrate reductase activity independent of the presence of nitrate. On the other hand, nitrite reductase activity was dependent not only on low levels of oxygen but also on the presence of a high nitrate concentration in the medium. Denitrification activity proceeded immediately once a threshold level of nitrite was accumulated in the medium or in cells incubated with 1mM nitrite. However, a lag period was required when cells were incubated with nitrate.
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  • 43
    ISSN: 1573-5036
    Keywords: allantoic acid ; allantoin ; ammonium ; nitrate ; common bean ; 15N dilution analyses ; xylem ureide assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Utilising the 15N dilution technique the relationship between the proportion of N derived from N2 fixation and relative abundance of ureides in xylem sap was evaluated for Phaseolus vulgaris L. cv. Mokcham during vegetative and reproductive development. In order to establish calibration curves for time integrated estimates of N2 fixation, plants were raised in sand culture during the dry season in northern Thailand and continuously supplied with a N-free nutrient solution or the same solution amended with 0, 3, 6 or 9 mol m−3 nitrate. Large changes in plant dependence on N2 fixation were concomitantly reflected by corresponding alterations in N solutes in xylem sap. Regression analyses of the data suggested high correlations between relative ureide content and N2 fixation, but different slopes and line intercepts indicated the requirement for the use of calibration curves established for different phases of the development of the plant. Largest age related differences were noted between vegetative and reproductive development. Judging from 95% confidence limits, utilisation of appropriate calibrations can reduce errors of the technique to close to ±5%. A second experiment, involving similarly cultivated plants exposed to different sources of mineral N, indicated an effect of ammonium on xylem sap composition. This implies that calibrations, in which N2 fixation is regulated only by applications of various concentrations of nitrate, may lead to errors in situations where a major proportion of the plant available soil nitrogen fraction is present in the form of ammonium.
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  • 44
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    Plant and soil 151 (1993), S. 127-138 
    ISSN: 1573-5036
    Keywords: urine ; nitrous oxide ; dinitrogen ; nitrification ; denitrification ; carbon ; 15N ; nitrification inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A 15N labelling technique was used to measure N2O and N2 emissions from an undisturbed grassland soil treated with cow urine and held at 30 cm water tension and 20°C in a laboratory. Large emissions of dinitrogen were detected immediately following urine application to pasture. These coincided with a rapid and large increase in soil water-soluble carbon levels, some of this increase being attributed to solubilization of soil organic matter by high pH and ammonia concentrations. Emissions of nitrous oxide generally increased with time in contrast to dinitrogen fluxes which decreased as time progressed. Estimated losses of N2O and N2 over a 30 day period were between 1 to 5% and 30 to 65% of the urine N applied plus N mineralized from soil organic matter, respectively. Most of the N2 and N2O originated from denitrification with nitrification-denitrification being of minor significance as a source of N2O. Comparisons of the 15N enrichments in the soil mineral N pools and the evolved N2O suggested that much of the N2O was produced in the 5–8 cm zone of the soil. It is concluded that established grassland soils contain large amounts of readily-oxidizable organic carbon which may be used by soil denitrifying organisms when nitrate is non-limiting and soil redox potential is lowered due to high rates of biological activity and high soil moisture contents. ei]{gnR}{fnMerckx}
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  • 45
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    Plant and soil 154 (1993), S. 67-72 
    ISSN: 1573-5036
    Keywords: ammonium uptake ; cereals ; kinetics ; legumes ; 15N ; nitrate uptake ; translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Influx isotherms were obtained for nitrate and ammonium from three legumes, Cajanus cajan (L.) Millsp., Cicer arietinum L. and Arachis hypogaea L. and three cereals, Sorghum bicolor (L.) Moench., Pennisetum glaucum L. and Zea mays L. The transition in influx isotherms for both nitrogen sources was found to be within the concentration range (0.05–2.5 mM) tested. There were significant differences in Km and Vmax for ammonium between legumes and cereals. The difference in the kinetic properties for nitrate uptake between the two groups of plants only became apparent at the higher concentration tested. Legumes translocated absorbed nitrate and ammonium to shoots more rapidly than cereals. Results show that there are significant differences in uptake and translocation of ammonium and nitrate between legumes and cereals.
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  • 46
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    Plant and soil 150 (1993), S. 167-175 
    ISSN: 1573-5036
    Keywords: ammonium nitrate ; barley ; fertilizer efficiency ; 15N field experiment ; N recovery ; 15N ; plant uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract 15N-labelled ammonium nitrate was applied to spring barley growing on a Cambisol soil in western Switzerland. Immobilization, plant uptake and disappearance of inorganic nitrogen were followed at frequent intervals. Fertilizer nitrogen disappeared shortly after its application, mainly through immobilization by soil microorganisms and absorption by the crop. Some of the added nitrogen was probably denitrified as a result of humid conditions during the first days after fertilizer application. At the end of the growing season, 31% of the added nitrogen was recovered from the aerial barley plants, and 56% was immobilized by microorganisms. Most of the fertilizer nitrogen not used by the crop was immobilized in the upper 0–30 cm soil layer. This prevented downward movement of nitrate and limited nitrogen losses. Fertilizer efficiency was mainly determined by the competition between crop uptake and microbial immobilization. Careful consideration of the time of fertilization, taking into account plant growth and weather conditions, can result in an increase in fertilizer efficiency and minimal pollution.
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  • 47
    ISSN: 1573-5036
    Keywords: lettuce ; maximum inflow (Imax) ; model ; nitrate ; relative growth rate ; root:shoot-ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lettuce was grown in nutrient solution under fluctuating climatic conditions in the greenhouse. The maximum nitrate inflow (Imax) was measured twice a week to validate a model for calculating Imax, that was developed for constant conditions in a growth chamber. Growth and Imax were very similar between greenhouse and growth chamber plants, so that the model was able to predict Imax very precisely. The daily maximum nitrate inflow was calculated and its dependency on fluctuating temperature could be shown.
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  • 48
    ISSN: 1573-5036
    Keywords: clover ; grass ; leaching ; lysimeter ; nitrogen ; 15N ; nutrient balances ; nutrient uptake ; pasture ; subsoiling ; sulphur ; 35S
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Synthetic cow urine labelled with 35S and 15N was applied to large, undisturbed, monolith lysimeters sampled from subsoiled and non-subsoiled areas of a grass/clover pasture. For one year following the urine application, the lysimeters were subjected to a combination of natural rainfall, simulated rainfall and simulated flood irrigations. Drainage from the lysimeters was sampled regularly and monthly (approx.) pasture cuts taken. At the end of the year, the lysimeters were destructively sampled in 50 mm depth increments for soil analysis. Leachates, plant samples and soil samples were analysed for 35S and 15N. There were no significant differences in plant uptake of 35S and 15N between the subsoiled and nonsubsoiled lysimeters. Initially grass showed a higher degree of labelling than clover. Total amounts of 35S and 15N leached from the subsoiled lysimeters were approximately twice that leached from the nonsubsoiled ones. Leaching patterns differed substantially between the two nutrients. Total recoveries of 35S (in plants, leachates and soil extracts) accounted for 82% of the applied 35S for the subsoiled lysimeters and 72% for non-subsoiled ones. The unrecovered 35S is considered to have been incorporated into soil organic matter. Total recoveries of 15N (in plants, soil and leachates) were similar to those for 35S, but unrecovered 15N is attributed to loss by denitrification.
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  • 49
    ISSN: 1573-5036
    Keywords: ammonium ; conventional rice ; hybrid rice ; nitrate ; N uptake ; solution culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Although NH4 + has generally been accepted as the preferred N source for fertilising rice, some workers have concluded tha NO3 - is as effective as NH4 +. The present glasshouse study exmined the relative uptake of NH4 + and NO3 - from solution and cultures containing 5–120 mg N/L supplied as NH4NO3 by a hybrid rice (India) and a conventional rice cultivar (Japonica). At all levels of N supply, the hybrid rice had higher leaf area and higher rates of uptake of total N than the conventional cultivar. Net photosynthesis rates were similar for both cultivars at the highest rates of N supply, but were lower at 5–40 mg N/L for the hybrid cultivar than for the conventional cultivar. At all levels of N supply, the conventional rice cultivar absorbed more NH4 + than NO3 -. In contrast, the hybrid rice absorbed more NH4 + than NO3 - at the low levels of N supply (5–40 mg N/L), but more NO3 - than NH4 + at the high levels of at 80 and 120 mg N/L. It is concluded that the uptake of N by rice is under genetic control and also dependent on levels of N supply. Thus the appropriate form of N fertiliser for rice may depend on cultivar and rates of N supply.
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  • 50
    ISSN: 1573-5036
    Keywords: ammonium ; nitrate ; nitrification ; nitrogen ; old-growth forest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Old-growth forest stands of mixed species composition provide the opportunity to study species-specific influences on soil properties. We monitored rates of nitrogen mineralization, nitrification and an index of ammonium and nitrate uptake in a mixed old-growth stand of Douglas-fir (Pseudotsuga menziesii), western hemlock (Tsuga heterophylla) and western redcedar (Thuja plicata) over a two-year period. Litter and mineral soil (0–10-cm depth) were sampled adjacent to ten large trees of each species. After initial characterization of litter and soil, buried bags were incubated in both layers for ca. 2-month intervals. Soil and litter pH was lowest near western hemlocks. Nitrification, nitrate concentrations, and percent uptake as nitrate differed among the tree species; rates were highest near western redcedars. For all species, percent nitrification and nitrate uptake rate were higher in soil than in litter. The results indicate species-specific effects on ammonium and nitrate production and uptake within this forest type.
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  • 51
    ISSN: 1573-5036
    Keywords: Broadcast ; loam soil ; 15N ; N losses ; N recovery ; row application ; split dressing ; sugar beet ; winter wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Since 1986, the fate of fertilizer N (NH4NO3 or NaNO3) applied in field conditions on two main arable crops, winter wheat (Triticum aestivum) and sugar beet (Beta vulgaris), has been studied using 15N. Up to a rate of 200 kg ha-1 of N, mean recovery of fertilizer by winter wheat was 70%, provided it had been split applied. Single application (with or without dicyandiamid) was less effective. For sugar beet, in 1990, 1991 and 1992, 40% of fertilizer N was found in the crop at harvest when NH4NO3 had been broadcast at 100 to 160 kg N ha-1 at sowing time. For the same N rate, recovery was 50% when row applied near the seeds and 60% for 80 kg N ha-1. For the two experimental crops, residual fertilizer N in soil was exclusively organic. It ranged from 15 to 30% of applied N and was located in the 30 cm upper layer. Losses were generally lower with winter wheat (12%) than with sugar beet (20–40%) and could be ascribed to volatilization and denitrification. Soil derived N taken up by the plant was site and year dependent.
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  • 52
    ISSN: 1573-5036
    Keywords: exponential addition ; nitrate ; Plantago lanceolata L. ; relative uptake rate ; reproductive growth rate ; SAR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Culture experiments are described in which Plantago lanceolata L. was grown from seedling till flowering under steady state conditions of optimum or suboptimum nitrate nutrition. In the optimum treatment, plants had free access to nitrate. In two suboptimum treatments, nitrate was added with constant relative addition rates (RAR) of 0.18 or 0.10 d−1 during the phase of constant relative uptake rates (RUR) of the plants and then with RAR's that were reduced stepwise from 0.18 to 0.07 d−1 or 0.10 to 0.04 d−1 when nutrient absorption gradually decreased. Reduction of the RAR's was aimed at maintenance of a balance between RAR and RUR. External nitrate concentrations were measured to monitor the reductions. In the vegetative phase, the relative growth rate (RGR) and the root weight ratio (RWR) of P. lanceolata were constant. In the reproductive phase, RGR's were constant, but lower, and RWR's decreased. Concentrations of organic-N in leaves were stable during the experimental period while those of the peduncles were lower and decreased with time. The ratio of reproductive to vegetative weight increased linearly with time. A number of plant parameters varied with N supply. ei]Section editor: T W Rufty
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  • 53
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    Plant and soil 155-156 (1993), S. 359-362 
    ISSN: 1573-5036
    Keywords: 15N ; microbial biomass ; nitrogen uptake efficiency ; Oryza sativa ; soil nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Recent field studies on irrigated rice at the IRRI research farm indicate efficient use of fertilizer-N based on plant uptake of applied N, (estimated by N difference), and utilization of acquired N for increased grain yield. These findings contrast with 15N uptake in microplot studies which underestimate the actual increase in plant N from added fertiliser. Constraints other than uptake efficiency, however, may govern fertiliser-N efficiency in farmers fields. In a study of 44 farmers' fields in Central Luzon, rice yields ranged from 2.5 to 6.2 t ha-1 and N uptake from 35 to 95 kg N ha-1 in plots without fertiliser-N addition. Farmers applied from 35 to 240 kg N ha-1, but there was no relationship between the N rate used by each farmer and the effective soil N supply. Mean N uptake efficiency from fertiliser by N difference was only 36%. We conclude that improved fertiliser-N efficiency by farmers will require a more information-intensive management strategy that makes N fertiliser inputs better fitted to the seasonal pattern of crop N demand and soil N supply.
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  • 54
    ISSN: 1573-0867
    Keywords: Leaching ; calcium ; magnesium ; potassium ; nitrate ; tropical soils ; 15N ; urea ; shifting cultivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Calcium hydroxide was applied to monolith lysimeters at Onne in south-east Nigeria. Eight lysimeters were cropped with maize followed by upland rice and four were uncropped. The cropped and two uncropped lysimeters received Mg, K and urea in the first season. Two uncropped lysimeters received no fertilizers. Drainage water was collected during the two growing seasons and analyzed for calcium, magnesium, potassium, sodium, nitrate and chloride. The fertilizer applied in the second season was not leached during the year of application. The cropped lysimeters lost 27 percent of the sum of the exchangeable Ca in the soil profile and the calcium added, and 29 percent of the corresponding sum for Mg. With no crop, the losses increased to 34 and 37 percent, respectively, but with no crop or fertilizer, the losses were similar to those from the cropped lysimeters. The loss of potassium ranged from 6 percent from the unfertilized lysimeters to 10 percent in the cropped lysimeters. The amounts of sodium leached ranged from 29 to 35 kg Na ha−1. The bulk of the calcium and magnesium leached from calcium hydroxide and fertilizers occurred in the second season when the loss was in good agreement with the amount of nitrate lost giving (Ca + Mg)/NO3 charge ratios of approximately one. Urea increased the amount of nitrate leached and led to a corresponding increase in the amounts of calcium and magnesium lost in the drainage water. The charge ratio remained unchanged when the cations were leached only with nitrate derived from the mineralization of soil organic matter. In the cropped lysimeters, this source accounted for about four times more nitrate in the drainage water than the fertilizer.
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  • 55
    ISSN: 1573-0867
    Keywords: N-response ; model ; critical %N ; nitrate ; soil mineral-N ; N-loss ; leaching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To aid the development of simulation models for N-response, N-fertilizer experiments with onions (Allium cepa L.) were carried out on 5 different sites. In each experiment, there was little loss of fertilizer-N in soil during the period between application and rapid crop growth and little loss of mineral N by leaching at any time. Even so, a substantial proportion of the N applied as fertilizer could not be accounted for in the crop and soil at harvest; the sum of soil mineral-N plus crop N (excluding fibrous roots) was always linearly related to N rate applied over the entire range (0–300 kg N ha−1) and the gradient was always approximately the same, 0.64, irrespective of soil type or the amount of nitrate remaining in soil at harvest. Evidence was obtained that the phenomena resulted from roots retaining N and inducing immobilization at a rate proportional to soil nitrate concentration and that the proportionality constant was similar on all sites. Throughout plant growth there was little luxury consumption of N and the critical %N was related to plant mass by an equation previously deduced for other C3 crops (Plant and Soil 85, 163); plant nitrate concentration in the early stages increased with soil mineral-N (0–30 cm) to a maximum which varied from site to site but the nitrate concentration in the mature crop was always negligible. Plant yield in the early stages of growth generally declined with increase in fertilizer-N, despite the crops having been planted as sets and no more than 150 kg N ha−1 broadcast at one time; but at maturity, yield always increased asymptotically with increase in fertilizer-N. Mineralization rates were approximately the same in the first as in the second half of each experiment. At harvest, residual soil mineral-N in the upper 30, 60 and 90 cm of soil increased with increase in fertilizer-N even when crop demand for N exceeded supply. At harvest in every experiment, the ratio of crop dry weight in the absence of added N to the maximum obtained was approximately equal to the ratio of plant %N (with no fertilizer) to critical %N. The various phenomena concerning yields, plant-N contents, and values of soil mineral-N at harvest were quite well simulated by a slightly modified version of a previously published model (Fert. Res. 18, 153) with few site-dependent inputs.
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  • 56
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    Nutrient cycling in agroecosystems 32 (1992), S. 55-59 
    ISSN: 1573-0867
    Keywords: 15N ; nitrogen ; rice ; soil N ; N fertilizer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the southern U.S. rice belt it is recommended that rice (Oryza sativa L.) grown in the dry-seeded, delayed flood cultural system have the preflood N fertilizer applied and the field flooded at the fourth to fifth leaf stage of plant development. The objective of this field study was to determine if delaying the flood and preflood N application past the fifth leaf stage was detrimental to rice total N and fertilizer15N uptake, total dry matter, and grain yield. This study was conducted on a Crowley silt loam (Typic Albaqualfs) and a Perry clay (Vertic Haplaquepts). The preflood N fertilizer and flood were delayed 0, 7, 14, or 21 d past the fourth to fifth leaf stage, after which time a permanent flood was established and maintained until maturity. All treatments received 20.5 g N m−2 as15N-labeled urea in three topdress applications. All plant and soil samples were taken at maturity. Harvest index increased as the preflood N and flood were delayed past the 4 to 5 leaf stage. Total N in the grain + straw either decreased or showed a decreasing trend as the N and flood were delayed. Similarly, uptake of native soil N decreased as flood was delayed. Conversely, percent recovery of fertilizer N in the rice plant and the plant-soil system increased as the preflood N and flood were delayed. Rice grain yield was not significantly affected by delaying the preflood N and flood up to 21 d.
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  • 57
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    Nutrient cycling in agroecosystems 33 (1992), S. 71-79 
    ISSN: 1573-0867
    Keywords: Nitrogen balance ; 15N ; oxamide ; slow release fertilizer ; soil microbial biomass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Slow release N fertilizers are receiving increasing attention for use on turf grass, but their fate in the plant-soil system is still poorly understood. We aimed to quantify the uptake and recovery of N by a mixture of grasses when applied as either urea or oxamide in different diameter granules using a tracer technique (15N). The effects of the N source on soil biomass, root density and amount of readily available organic C in soil were also evaluated. In a first experiment oxamide in 4–5 mm diameter granules was compared with urea. The initial N absorption, 40 days after fertilization (d.a.f.), was higher for urea (23.5%) than for oxamide (12.1%), but after 64 days absorption efficiencies were about the same (11%) for both fertilizers. Fertilizer-derived N lost by leaching was much greater from the urea-fertilized soil (1.57 g), compared with losses from oxamide-fertilized soil (0.05 g). The total residual fertilizer N remaining in the system at the end of the experiment was 26.7% of applied urea N and 39.6% of applied oxamide N. Cumulated absorption efficiencies, calculated after dismantling the lysimeters, were 43.1% for urea and 54.8% for oxamide (roots included). A priming effect caused by a larger uptake of soil N because of the better root development was found in the oxamide-treated lysimeter. Fertilization with oxamide also caused an increase in the amount of soil microbial biomass. In a second experiment, the efficiencies and fertilizer N uptake rates from oxamide applied at two different granule sizes (1–2 mm and 5–10 mm) were evaluated. The amount of soil N taken up by the grass was linearly related to root density (r = 0.92).
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  • 58
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    Biology and fertility of soils 12 (1992), S. 241-252 
    ISSN: 1432-0789
    Keywords: Decomposition ; Litter ; Microarthropods ; Nitrogen ; 15N ; Litterbags ; Cornus florida ; Quercus prinus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Surface additions of (15NH4)2SO4 were used to measure the immobilization and subsequent movement of exogenous N added to two litter types of contrasting quality (Cornus florida and Quercus prinus). Litterbaskets were used to measure the litter mass loss and N dynamics and to follow the movement of the 15N label through litter, F layer, and soil pools. Half of the litterbaskets of each species were treated with naphthalene to reduce microarthropod densities. The faster decomposing C. florida litter maintained a higher excess atom % 15N, and a greater relative concentration of the labeled input (μg 15N g−1) than did Q. prinus litter. In both litter types the excess atom % 15N, relative concentration (μg 15N g−1), and absolute amount of label recovered in the litter declined over time. This occurred during a period of net accumulation of total litter N, implying simultaneous release of the initial input and immobilization of N from other sources. The concentration of 15N in the soil increased over time, while the F layer apparently acted as an intermediary in the transfer of 15N from litter to soil. Naphthalene effectively reduced microarthropod numbers in all horizons of the litterbaskets and significantly reduced the decay rates of Q. prinus, but not C. florida litter. Naphthalene did not appear to affect total N dynamics in the litter. However, with all horizons taken together, the naphthalene-treated litterbaskets retained more total 15N than the control litterbaskets. Naphthalene also changed the vertical distribution of 15N within litterbaskets, so that the litter retained less of the 15N-labeled input and the F layer and soil horizons retained more of the labeled input than in control litterbaskets. Our major conclusions are: (1) the N pool of decomposing litter is dynamic, with simultaneous N release and immobilization activating N turnover even during the net accumulation phase; (2) litter quality is an important determinant of immobilization and retention of exogenous N inputs and, therefore, turnover of the litter N pool; and (3) microarthropod activity can significantly affect the incorporation and retention of exogenous N inputs in decomposing litter, although these changes are apparently not reflected in net N accumulation or release during the 1st year of decomposition. However, the naphthalene may have affected microbially mediated N dynamics and this possibility needs to be considered in interpreting the results.
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  • 59
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    Biology and fertility of soils 13 (1992), S. 1-5 
    ISSN: 1432-0789
    Keywords: Nitrification inhibitor ; Dicyandiamide ; 15N ; Incubation ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of temperature on the action of a dicyandiamide nitrification inhibitor was studied during a laboratory incubation after the addition of ammonium sulphate labelled with 15N. In the control treatment, nitrification was only slightly affected by temperature and was rapid; on the 42nd day, two-thirds of the 15N was incorporated into the nitrate fraction while no further tracer was found in ammoniacal form. With the addition of dicyandiamide, the process was slowed down considerably when the temperature was maintained at 10°C, and only about 10% of the 15N was nitrified in 6 months. After 1 month of incubation at 10°C, a temperature increase to 15°C for 4 weeks modified the nitrification kinetics only slightly. However, as soon as the temperature reached 20°C, the beginning of dicyandiamide decomposition and an increase in the quantity of NO 3 - -N was observed. The inhibition was measured by the nitrification index, which was greater than 80% as long as the temperature did not exceed 15°C, and decreased to 10% after 6 months; this value was reached only after 1 year in soil maintained at 10°C. The half-life of the NH 4 + was decreased by raising the temperature. In the experimental conditions described, nitrification was inhibited by the dicyandiamide for at least 6 months provided the temperature did not exceed 15°C.
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  • 60
    ISSN: 1432-0789
    Keywords: Typic cryoboroll ; N yield ; 15N ; Root length ; Grass-legume intercrop ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Barley-field pea intercrops have been shown to increase N yield when grown under cryoboreal subhumid conditions. In this study, we extended previous research by testing the hypotheses that (1) the intercropped field pea fixes a greater proportion of its shoot and root N than does sole-cropped field pea; (2) N is transferred from the annual legume to the cereal during the growing season; and (3) root production is greater under intercropped than sole-cropped conditions. Unconfined microplots seeded to barley, field peas, or a barley-field pea intercrop were fertilized with N at 10 kg ha-1 as (NH4)2SO4 (5.21 atom % 15N excess). Both the intercropped and sole-cropped barley derived more than 93% of their N from the soil. In contrast, 40% of N in the intercropped field pea was derived from soil. This study provided no evidence for transfer of N from the legume to the cereal. On average, the proportion of N derived from air by both pea intercrops was 39% higher than that derived by the sole-cropped pea. Root length determined by a grid intersection method following digitization using an image analyzer tended to be higher under intercropping than in sole crops. We conclude that even on fertile soils benefits may accrue from annual intercropping that includes a legume. The benefits arise from (1) increased N production, (2) greater N-fixation efficiency, and/or (3) more shoot and root residue-N mineralization for subsequent crops.
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  • 61
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    Potato research 35 (1992), S. 355-364 
    ISSN: 1871-4528
    Keywords: cultivar ; nitrate ; nitrogen ; Solanum tuberosum L. ; tuber
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The duration of dormancy of seed potatoes varies between years and between origins. Therefore, the effects of conditions during crop growth on dormancy of progeny tubers were studied. The effect of nitrogen during tuber bulking on the duration of dormancy was investigated in three field experiments with two cultivars. In addition to an application of 125 kg N/ha at planting, top dressings of 0–150 kg N/ha were given about 2 weeks after tuber initiation. Haulm was pulled about 4 weeks later. The effect of nitrogen rate at planting was also examined in one experiment. Nitrogen top dressings shortened dormancy in all experiments by 5–8 days. An increased nitrogen rate at planting resulted in a shorter dormancy when the duration of dormancy was expressed in days after tuber initiation, but not when it was expressed in days after haulm pulling, probably because extra nitrogen also delayed tuber initiation.
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  • 62
    ISSN: 1573-5036
    Keywords: ammonium ; Ca-phosphate ; Fe/Al-phosphate ; nitrate ; P mobilization ; rhizosphere pH ; ryegrass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mobilization of soil P as the result of plant-induced changes of soil pH in the vicinity of plant roots was studied. Seedlings of ryegrass were grown in small containers separating roots from soil by a 30-μm meshed nylon screen which root hairs could penetrate but not roots. Two soils were used, a luvisol containing P mainly bound to calcium and an oxisol containing P mainly bound (adsorbed) to iron and aluminum. Plant-induced changes of soil pH were brought about by application of ammonium-or nitrate-nitrogen. After plants had grown for 10 d the soil was sliced in thin layers parallel to the root mat which had developed on the screen, and both soil pH and residual P determined. Mobilization of P was assessed by P-depletion profiles of the rhizosphere soil. Soil pH at the root surface decreased by up to 1.6 units as the result of ammonium N nutrition and it increased by up to 0.6 units as the result of nitrate N nutrition. These changes extended to a distance between 1 and 4 mm from the root surface depending on the type of soil and the source and level of nitrogen applied. In the luvisol, compared to zero-N treatment, P mobilization increased with the NH4-induced decrease in pH, whereas the NO3-induced pH increase had no effect. In contrast, in the oxisol a similar pH decrease caused by NH4 nutrition had no effect, whereas the pH increase caused by NO3 increased markedly the mobilization of soil P. It is concluded that in the luvisol calcium phosphates were dissolved by acidification, whereas in the oxisol adsorbed phosphate was mobilized by ligand exchange.
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  • 63
    ISSN: 1573-5036
    Keywords: early-successional forest ; gross N mineralization ; moist tropics ; N immobilization ; 15N ; nutrification ; nutrient cycling ; old-growth forest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We compared the resin-core and buried-bag incubation methods for estimating nitrogen (N) transformation rates using the 15N pool dilution technique in alluvial soils of an early successional forest (ESF) and an old-growth forest (OGF) at the La Selva Biological Station in Costa Rica. Soil cores (38×100-mm) from both forests were incubated in situ for 7 days. The two methods gave generally similar estimates of net N mineralization rates for the two forests. Estimates of ammonium production by the resin-core method were higher than those by the buried-bag method in ESF, but did not differ significantly in OGF (p〈0.05). Estimates of nitrate production by the two methods did not differ significantly. Nitrate averaged 74% and 81% of the total inorganic N production in ESF and OGF, respectively. Net N mineralization in ESF (6.6 mmol m-2d-1) did not differ significantly from that in OGF (5.0 mmol m-2d-1). Fluxes of ammonium and nitrate were high for both forests, but the OGF tended to have higher gross mineralization and nitrification rates than ESF. Approximately 60% of the gross nitrate production and less than 30% of the ammonium were immobilized by microorganisms.
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  • 64
    ISSN: 1573-5036
    Keywords: Fagus silvatica L. ; wet deposition ; N-uptake ; 15N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Uptake of NH4 and NO3 by above ground parts of beech trees was studied by spraying young trees with varying concentrations of 15N labeled solutions, different N-forms, and spray regimes over four months. Following treatment, the trees were harvested and analyzed for 15N and major element content. Throughfall was collected and analyzed in addition in order to study the interaction between nitrogen uptake and cation leaching. Significant amounts of N were taken up by the above ground plant parts in all treatments as indicated by 15N analysis of the trees as well as by throughfall measurements. NH4 uptake exceeded the uptake of NO3 if applied in the same concentration. Uptake of N increased linearly with increasing concentration in the spray solution and with spray intensity. The uptaken N was translocated within the plant. The contribution of N from uptake by above ground parts to the total N content of tissues differed and reached a maximum level of 6% in leaves. No effect of above ground N uptake on the total N content of tissues was found. Calculating atmospheric N inputs to forest ecosystems by throughfall measurements may underestimate the actual N input.
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  • 65
    ISSN: 1573-5036
    Keywords: biological nitrogen fixation ; denitrification ; fallow ; flooded soil ; leaching ; legume ; nitrate ; nitrogen balance ; nitrogen loss ; Oryza sativa ; rice ; weeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lowlands comprise 87% of the 145 M ha of world rice area. Lowland rice-based cropping systems are characterized by soil flooding during most of the rice growing season. Rainfall distribution, availability of irrigation water and prevailing temperatures determine when rice or other crops are grown. Nitrogen is the most required nutrient in lowland rice-based cropping systems. Reducing fertilizer N use in these cropping systems, while maintaining or enhancing crop output, is desirable from both environmental and economic perspectives. This may be possible by producing N on the land through legume biological nitrogen fixation (BNF), minimizing soil N losses, and by improved recycling of N through plant residues. At the end of a flooded rice crop, organic- and NH4-N dominate in the soil, with negligible amounts of NO3. Subsequent drying of the soil favors aerobic N transformations. Organic N mineralizes to NH4, which is rapidly nitrified into NO3. As a result, NO3 accumulates in soil during the aerobic phase. Recent evidence indicates that large amounts of accumulated soil NO3 may be lost from rice lowlands upon the flooding of aerobic soil for rice production. Plant uptake during the aerobic phase can conserve soil NO3 from potential loss. Legumes grown during the aerobic phase additionally capture atmospheric N through BNF. The length of the nonflooded season, water availability, soil properties, and prevailing temperatures determine when and where legumes are, or can be, grown. The amount of N derived by legumes through BNF depends on the interaction of microbial, plant, and environmental determinants. Suitable legumes for lowland rice soils are those that can deplete soil NO3 while deriving large amounts of N through BNF. Reducing soil N supply to the legume by suitable soil and crop management can increase BNF. Much of the N in legume biomass might be removed from the land in an economic crop produce. As biomass is removed, the likelihood of obtaining a positive soil N balance diminishes. Nonetheless, use of legumes rather than non-legumes is likely to contribute higher quantities of N to a subsequent rice crop. A whole-system approach to N management will be necessary to capture and effectively use soil and atmospheric sources of N in the lowland rice ecosystem.
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  • 66
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    Plant and soil 143 (1992), S. 115-125 
    ISSN: 1573-5036
    Keywords: N-mineralization ; nitrification ; nitrate ; radiata pine ; deformation ; tree form
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies of nitrogen availability were carried out in radiata pine (Pinus radiata D. Don) plantations on former pasture sites in N.S.W. in conjunction with studies of the effects of previous land use on tree form. Sites were selected on previously improved pastures (cleared with introduced legumes) and unimproved pastures (partially cleared without legumes) to form age sequences of stands which had been established for periods of up to fifteen years. Mineral-N pools in soils and forest floor samples were determined monthly for thirteen months and nitrification potentials were determined from periodic laboratory incubations. Nitrate and ammonium pools in 2-, 4-, 6-, 9- and 15-year-old radiata pine stands fluctuated seasonally, peaking in summer and autumn. Maximum total mineral-N concentrations of 20 to 40 μg g−1 soil occurred in the youngest, ex-improved pastures with nitrate-N concentrations of up to 25 μg g−1. In the 15-year-old stands, nitrate-N was only detected during autumn, at less than 5 μg g−1 soil. Net N-mineralization and nitrification potentials were consistently higher in the ex-improved pasture soils compared with the ex-unimproved pastures. N availability decreased with increasing stand age in the ex-improved pasture soils, but the pattern was less clear for the unimproved pasture sites. Suppression of clover by pines and the accumulation of nitrogen in the standing biomass are thought to be the major factors controlling the decline of available N during stand development.
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  • 67
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    Plant and soil 144 (1992), S. 289-295 
    ISSN: 1573-5036
    Keywords: ammonium ; ammonia toxicity ; maize (Zea mays L.) ; nitrate ; nitrogen use efficiency ; root length density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Growth of maize seedlings can be improved by enhanced ammonium nutrition, but placing fertilizer anhydrous ammonia close to seedlings introduces the risk of ammonia toxicity. In this study, growth and root elongation response to rates of closely placed NH4OH bands were investigated in two contrasting maize hybrids. Seven rates of NH4OH, ranging from 0 to 200 mg N kg-1 soil were injected into the center of each pot. A single rate of Ca(NO3)2-N was included to compare hybrids for N form preference at a moderate N rate. Three seedlings per pot were planted 5.7 cm from the injection point. Hybrid B73×LH51 produced a quadratic response in shoot growth to NH4OH rates, whereas LH74×LH123 exhibited a significant linear decline in response to NH4OH rate. Root length density sampled from the fertlized zone declined linearly in response to NH4OH rate while a slight increase in root length density in unfertilized zones was observed at intermediate NH4OH rates. Hybrids did not differ in root length density in either zone. The hybrid with greater tolerance of NH4OH rates (B73×LH51) also showed a preference in shoot growth for NH4-over NO3-N at 66.7 mg N kg-1 compared to LH74×LH123. On average across hybrids, nitrate concentrations of xylem exudate collected from detopped plants were 14.5 mmol g-1 for Ca(NO3)2 treatments and 1.5 mmol g-1 for NH4OH treatments, indicating that contrasting N-form nutrition resulted from fertilizer treatments. Malate concentrations were higher in the NH4OH treatment indicating that this organic acid anion may substitute for the negative charge of nitrate during enhanced ammonium nutrition in maize. The results suggest that potentially useful genetic variation exists in maize for N form preference and for tolerance to increasing ammonical-N rates.
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  • 68
    ISSN: 1573-5036
    Keywords: ammonium ; cyanides ; Gaeumannomyces graminis var tritici ; nitrate ; nitrogen fertilizer ; pseudomonads ; soil conduciveness ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field cropped with wheat, a high and low level of soil conduciveness to take-all were induced by applying a nitrogen fertilizer with either calcium nitrate or ammonium sulphate. From these two soils, two representative populations of fluorescent pseudomonads were tested for their in situ behaviour. Take-all index and root dry weight were assessed on plants cropped in soils infested with Gaeumannomyces graminis var tritici (Ggt) and each bacterized with one of the isolates of fluorescent pseudomonads. The bacteria tested can be split into three groups: antagonists which reduce take-all, deleterious isolates which aggravate the disease and neutral without evident effect on the disease. The predominance of antagonistic fluorescent pseudomonads in the NH4-treated soil and the predominance of deleterious ones in the NO3-treated soil was confirmed after statistical analysis. The microbial impact on take-all must be more considered as the resulting effect of divergent activities of both rhizobacteria types than the only consequences of the presence of antagonistic pseudomonads. All the high cyanogenic pseudomonads were antagonists in situ and were more numerous in the NH4-treated soil than in the NO3-treated soil.
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  • 69
    ISSN: 1573-5036
    Keywords: ammonium ; Gaeumannomyces graminis var tritici ; nitrate ; nitrogen fertilizers ; fluorescent pseudomonads ; root system ; soil conduciveness ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Take-all of wheat, caused by Gaeumannomyces graminis var tritici (Ggt), is reduced by ammoniacal fertilizers as compared to nitrate sources. This influence of nitrogen on the disease is only observed on nodal roots at flowering. But soil conduciveness to take-all, as measured in a soil bioassay, is modified earlier. Forty days after nitrogen application at early tillering, the NH4-treated soil became less conducive than the NO3-treated one. When nitrogen applications are done at sowing and at tillering, differences in disease propagation between the two soils are enhanced. Results from four years of experimentation show that when the level of natural soil inoculum is high, disease severity is reduced by ammonium, showing an effect on the parasitic phase of Ggt. At a low level of natural inoculum the effect of the source of nitrogen is mainly observed on the percent of infected plants, indicating that the saprophytic and preparasitic phases are affected. Rhizospheric bacterial populations increase from sowing to tillering, but differences on take-all conduciveness after tillering are not correlated with differences in the amounts of aerobic bacteria or fluorescent pseudomonads isolated from soils treated with different sources of nitrogen. Qualitative changes in fluorescent Pseudomonas spp. populations, like in vitro antagonism, are more likely to explain differences in soil conduciveness to take-all than are quantitative changes in this group. Nevertheless, the introduction of Ggt in a cropped soil leads to a greater increase in fluorescent pseudomonads populations than in total aerobic bacteria. The delay between reducing soil conduciveness and reducing disease in the field with ammonium nitrogen fertilization, the qualitative change of fluorescent pseudomonads populations and the role of necroses in rhizobacteria multiplication, provide information leading to our representation of a dynamic model based on the differentiation of the wheat root system into seminal and nodal roots.
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  • 70
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    Plant and soil 147 (1992), S. 49-57 
    ISSN: 1573-5036
    Keywords: ammonium ; field ; kiwifruit ; 15N ; nitrogen fertilizer ; recovery ; soil nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The fate of 15N-labelled ammonium fertilizer applied once to six-year-old field-grown kiwifruit (Actinidia deliciosa ‘Hayward’) vines was measured over three years. The three main treatments were nitrogen (N) applied singularly at 100 or 200 kg N ha−1 in early spring (two weeks before bud burst) or split with 100 kg N ha−1 (unlabelled) in early spring and 100 kg N ha−1 (15N-labelled) ten weeks later. All N treatments were applied to vines with a history of either 50 or 200 kg N ha−1 yr−1. For three years after 15n application, components of the vines and soil (0–600 mm depth) were sampled at harvest in late autumn and the N and 15N contents determined. By the first harvest, all plant uptake of 15N had occurred and this represented 48–53% of the 15N applied. There was no significant effect of current N fertilizer treatment or of N history on 15N recovery by vines. Removal of 15N in harvested fruit was small at 5–6% in the first year and 8% over 3 years. After 2–3 years, most plant 15N occurred in the roots and this component declined only slowly over time. In contrast, there was a large temporal decline in 15N in above-ground plant components due to the annual ‘removal’ in leaf fall and pruning. An associated experiment showed that when 15N-labelled prunings and leaves were mulched and returned to the soil, only about 9% was recovered by plants within 2 years. Almost all remaining mulched material had been immobilised into the soil organic N. In all treatments, about 20% of the added 15N remained in soil at the first harvest. This was almost entirely in organic fractions (〈0.4% in inorganic N) and mostly in the surface 150-mm layer. The 15N content in soil changed little over time (from 20 to 17% between the first and third harvests respectively) and indicated that most of the N had been immobilised into stable humus forms.
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  • 71
    ISSN: 1573-5036
    Keywords: ammonium ; citrus ; nitrate ; NO3 −/NH4 + ratio ; nitrogen ; nutrient solution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In short-term water culture experiments with different 15N labeled ammonium or nitrate concentrations, citrus seedlings absorbed NH4 + at a higher rate than NO3 −. Maximum NO3 − uptake by the whole plant occurred at 120 mg L−1 NO3 −-N, whereas NH4 + absorption was saturated at 240 mg L−1 NH4 +-N. 15NH4 + accumulated in roots and to a lesser degree in both leaves and stems. However, 15NO3 − was mostly partitioned between leaves and roots. Adding increasing amounts of unlabeled NH4 + (15–60 mg L−1 N) to nutrient solutions containing 120 mg L−1 N as 15N labeled nitrate reduced 15NO3 − uptake. Maximum inhibition of 15NO3 − uptake was about 55% at 2.14 mM NH4 + (30 mg L−1 NH4 +-N) and it did not increase any further at higher NH4 + proportions. In a long-term experiment, the effects of concentration and source of added N (NO3 − or NH4 +) on nutrient concentrations in leaves from plants grown in sand were evaluated. Leaf concentration of N, P, Mg, Fe and Cu were increased by NH4 + versus NO3 − nutrition, whereas the reverse was true for Ca, K, Zn and Mn. The effects of different NO3 −-N:NH4 +-N ratios (100:0, 75:25, 50:50, 25:75 and 0:100) at 120 mg L−1 total N on leaf nutrient concentrations, fruit yield and fruit characteristics were investigated in another long-term experiment with plants grown in sand cultures. Nitrogen concentrations in leaves were highest when plants were provided with either NO3 − or NH4 + as a sole source of N. Lowest N concentration in leaves was found with a 75:25 NO3 −-N/NH4 +-N ratio. With increasing proportions of NH4 + in the N supply, leaf nutrients such as P, Mg, Fe and Cu increased, whereas Ca, K, Mn and Zn decreased. Yield in number of fruits per tree was increased significantly by supplying all N as NH4 +, although fruit weight was reduced. The number of fruits per tree was lowest with the 75:25 NO3 −-N:NH4 +-N ratio, but in this treatment fruits reached their highest weight. Rind thickness, juice acidity, and colour index of fruits decreased with increasing NH4 + in the N supply, whereas the % pulp and maturity index increased. Percent of juice in fruits and total soluble solids were only slightly affected by NO3 −:NH4 + ratio.
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  • 72
    ISSN: 1573-5036
    Keywords: ammonium ; ammonitrate ; Coffea arabica L. ; Glomus intraradices ; nitrate ; nutrient uptake ; pH ; rhizosphere ; vesicular arbuscular mycorrhizae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effects of N sources (ammonium, nitrate and ammonitrate) and VA mycorrhizae (Glomus intraradices) on rhizosphere soil characteristics (pH, exchangeable acidity, exchangeable cations, inorganic N concentrations) growth and nutrient acquisition of coffee seedlings (Coffea arabica L. cv guatemala) were investigated in a pot study with an acid soil (Red Bluff Loam) sterilized by autoclaving. Ammonium addition decreased rhizosphere pH while nitrate and ammonitrate additions both increased rhizosphere pH. Mycorrhizae induced a higher pH, a lower exchangeable acidity and higher values of exchangeable cations in the rhizosphere. Ammonium addition resulted in a lower mycorrhizal infection than the two other N sources. Mycorrhizal plants grew better and accumulated more N, Ca and Mg than non-mycorrhizal plants.
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  • 73
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    Plant and soil 139 (1992), S. 247-251 
    ISSN: 1573-5036
    Keywords: ammonium ; lupins ; nitrate ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two cultivars of Lupinus angustifolius L. were grown in a glasshouse in solutions containing NO3 -, NH4 + or NH4NO3 with a total nitrogen concentration of 2.8 M m-3 in each treatment. One cultivar chosen (75A-258) was relatively tolerant to alkaline soils whereas the other (Yandee) was intolerant to alkalinity. Controlled experiments were used to assess the impact of cationic vs. anionic forms of nitrogen on the relative performance of these cultivars. Relative growth rates (dry weight basis) were not significantly different between the two cultivars when grown in the presence of NO3 -, NH4 + or NH4NO3. However, when NO3 - was supplied, there was a modest decline in relative growth rates in both cultivars over time. When plants grown on the three sources of nitrogen for 9 days were subsequently supplied with 15NH4NO3 or NH4 15NO3 for 30 h, NH4 + uptake was generally twice as fast as NO3 - uptake, even for plants grown in the presence of NO3 -. Low rates of NO3 - uptake accounted for the decrease in growth rates over time when plants were grown in the presence of NO3 -. It is concluded that the more rapid growth of 75A-258 than Yandee in alkaline conditions was not due to preferential uptake of NH4 + and acidification of the external medium. In support of this view, acidification of the root medium was not significantly different between cultivars when NH4 + was the sole nitrogen source.
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  • 74
    ISSN: 1573-5036
    Keywords: 15N ; forest decline ; nitrogen deposition ; revitalization fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract 15N abundances of current needles of Norway spruce collected during 23 yrs of a forest fertilization experiment were studied in order to follow ecosystem gains and losses of N. Unlabelled ammonium nitrate at four rates (N0–N3), phosphorus at three rates (P0–P2), and potassium plus other elements including micronutrients at two rates (K0–K1), had been applied to plots in a complete factorial design. Nitrogen had been applied annually at average rates of 0, 34, 68 and 102 kg N ha-1 yr-1. Tree growth had responded positively to additions of N, but the response was remarkably more positive to the N2P2K1 treatment. In N1 treatments, δ15N (‰) declined over time. This was consistent with an earlier study, and should reflect a change in 15N abundance towards that of fertilizer N (minus discrimination during uptake), which in turn means accretion of most of the N added. As in the earlier study, in which N3 plots lost most of the N added, the present N3 plots showed an increasing δ15N (‰). This pattern was not significantly affected by additions of P and K plus other elements, although a weak negative effect of P on N accretion was indicated, i.e. there was a tendency δ15N (‰) to be higher when P was added. This, and another recent result based on an N budget, shows that so-called revitalization fertilization may well increase growth of trees, but also promotes losses of N from the ecosystem. As in the previous study, a decline in δ15N (‰) on control plots provided evidence of contamination. Given a removal of 100 kg N ha-1 at stem harvest and a leaching of 2 kg N ha-1 yr-1, our data on 15N suggest that a load of 9 kg N ha-1 yr-1 would saturate the ecosystem after 100 years. This load is only about twice the annual deposition at the site.
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  • 75
    ISSN: 1573-5036
    Keywords: critical concentration ; deficiency ; diagnosis ; growth rate ; lettuce ; luxury consumption ; nitrogen ; nitrate ; nutrient requirement ; petiole sap ; phosphorus ; phosphate ; potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A method is described for determining the way in which growth rate varies with plant nutrient concentration using a simple nutrient interruption technique incorporating only 2 treatments. The method involves measuring the changes in growth and nutrient composition of otherwise well-nourished plants after the supply of one particular nutrient has been withheld. Critical concentrations are estimated from the relationship between the growth rate (expressed as a fraction of that for control plants of the same size which remained well-nourished throughout) and the concentration of the growth-limiting nutrient in the plants as deficiency developed. Trials of the method using young lettuce plants showed that shoot growth rate was directly proportional to total N (nitrate plus organic N) concentration, and linearly or near-linearly related to K and P concentration over a wide range; the corresponding relationship for nitrate was strongly curvi-linear. Critical concentrations (corresponding to a 10% reduction in growth rate) determined from these results were similar to critical values calculated from models derived from field data, but were generally higher than published estimates of critical concentration (based on reductions in shoot weight) for plants of a similar size. Reasons for these discrepancies are discussed. Nitrate, phosphate or potassium concentrations in sap from individual leaf petioles were highly sensitive to changes in shoot growth rate as deficiency developed, with the slope of the relationships varying with leaf position, due to differences both in their initial concentration and in the rates at which they were utilized in individual leaves. Each nutrient was always depleted more quickly in younger leaves than in older ones, providing earlier evidence of deficiency for diagnostic purposes. Although the plants were capable of accumulating nitrate, phosphate and potassium well in excess of that needed for optimum dry matter production during periods of adequate supply, the rate of mobilization of these reserves was insufficient to prevent reductions in growth rate as the plants became deficient. This brings into question the validity of the conventional concept that luxury consumption provides a store of nutrients which are freely available for use in times of shortage. The implications of these results for the use of plant analysis for assessing plant nutrient status are discussed.
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  • 76
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    Plant and soil 143 (1992), S. 19-31 
    ISSN: 1573-5036
    Keywords: ammonium ; calcium ; carboxylate/organic nitrogen ratio ; 15N isotope ; nitrate ; nitrogen cycling ; perennial ryegrass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Solution culture studies have shown that plant uptake of NH4 + and NO3 - can be improved by increasing the concentration of Ca2+ in the root environment: the same may be true for grass grown in soil culture. An experiment was set up to see whether gypsum (CaSO4 2H2O) increased the rate at which perennial ryegrass absorbed 15NH4 + and 15NO3 - from soil. The results demonstrated that gypsum increases the rates of uptake of both NH4 + and NO3 - by perennial ryegrass. However because there was little potential for mineral-N loss from the experimental system, either by gaseous emission or by N immobilization, long term improvements in fertilizer efficiency were not observed. Nitrogen cycling from shoots to roots commenced once net uptake of N into plants had ceased. Labelled N transferred thus to roots underwent isotopic exchange with unlabelled soil N. It was suggested that this exchange of N might constitute an energy drain from the plant, if plant organic N was exchanged for soil inorganic N. The fact that the exchange occurred at all cast doubt on the suitability of the 15N-isotope dilution technique for assessing fertilizer efficiency in medium to long term experiments. There was evidence that the ‘extra’ NO3 --N taken up by plants on the all-nitrate treatments as a result of gypsum application, was reduced in root tissue rather than in shoots, but to the detriment of subsequent root growth and N uptake.
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  • 77
    ISSN: 1573-5036
    Keywords: ammonia toxicity ; chloride ; inhibition ; nitrate ; nitrification ; salt stress ; urine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of urinary chloride and nitrogen concentration and osmotic pressure on the nitrification of ammonium in a calcareous soil treated with cow urine were examined. Urinary chloride concentrations of up to 7.4 g L−1 had no effect on the rate of nitrification, as determined by the accumulation of soil nitrate. Osmotic stress, generated using a mixed salt solution, had an inhibitory effect on nitrification at soil osmotic pressures lower than or equal to −1.0 PMa. Nitrification was completely inhibited at a soil osmotic pressure of −2.6 MPa. Accumulation of nitrate after a lag phase of 18 days was noted in the −2.0 MPa soil osmotic pressure treatment, indicating some degree of adaptation or osmo-regulation within the nitrifying population at this stress level. High urine-N concentrations resulted in considerable nitrite accumulations and reduced nitrification activity through the effect of free ammonia. It is concluded that in most temperate grassland soils at near-neutral pH, urinary chloride and nitrogen are unlikely to reduce nitrification rates, except where urine-N concentrations exceed 16 g N L−1. Inhibition due to osmotic stress will be directly related to soil moisture status and may be particularly severe in dry, light-textured soils.
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  • 78
    ISSN: 1573-5036
    Keywords: 15N ; nitrogen harvest index ; nitrogen uptake efficiency ; soil water potential ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was conducted to investigate the influence of soil water potential, depth of N placement, timing, and cultivar on uptake of a small dose of labeled N applied after anthesis by wheat (Triticum aestivum L.) Understanding postanthesis N accumulation should allow better control of grain protein concentration through proper manipulation of inputs. Two hard, red spring-wheat cultivars were planted in early and late fall each yr of a 2-yr field experiment. Less than 1 kg N ha−1 as K 15NO3 was injected into the soil at two depths: shallow (0.05 to 0.08 m) and deep (0.15 to 0.18 m). In both years an irrigation was applied at anthesis, and injections of labeled N were timed 4, 12, and 20 days after anthesis (DAA). Soil water potential was estimated at the time of injection. Mean recovery of 15N in grain and straw was 57% of the 15N applied. Recovery did not differ between the high-protein (Yecora Rojo) and the low-protein (Anza or Yolo) cultivars. Mean recovery from deep placement was 60% versus only 54% from shallow placement (p 〈 0.01). Delaying the time of injection decreased mean recovery significantly from 58% at 4 DAA to 54% at 20 DAA. This decrease was most pronounced in the shallow placement, where soil drying was most severe. Regressions of recovery on soil water potential of individual cultivar x yr x planting x depth treatments were significant only under the driest conditions. Stepwise regression of 15N recovery on soil water potential and yield parameters using data from all treatments of both years resulted in an equation including soil water potential and N yield, with a multiple correlation coefficient of 0.64. The translocation of 15N to grain was higher (0.89) than the nitrogen harvest index (0.69), and showed a highly significant increase with increase in DAA. This experiment indicates that the N uptake capacity of wheat remains reasonably constant between 4 and 20 DAA unless soil drying is severe.
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  • 79
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    Plant and soil 141 (1992), S. 177-196 
    ISSN: 1573-5036
    Keywords: A-value ; acetylene reduction assay ; agroforestry ; isotope dilution ; 15N ; nitrogen fixation ; nodules ; trees ; ureide technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The integration of trees, especially nitrogen fixing trees (NFTs), into agroforestry and silvo-pastoral systems can make a major contribution to sustainable agriculture by restoring and maintaining soil fertility, and in combating erosion and desertification as well as providing fuelwood. The particular advantage of NFTs is their biological nitrogen fixation (BNF), their ability to establish in nitrogen-deficient soils and the benefits of the nitrogen fixed (and extra organic matter) to succeeding or associated crops. The importance of NFTs leads to the question of how we can maximise or optimize their effects and how we can manage BNF and the transfer of nitrogen to associated or succeeding plantings. To be able to achieve these goals, suitable methods of measuring BNF in trees are necessary. The total nitrogen difference (TND) method is simple, but is better suited for low than high soil N conditions. The acetylene reduction assay (ARA), although sensitive and simple, has many technical limitations especially for NFTs, and the estimates of BNF have generally been very low, compared to other methods. For NFTs, the 15N techniques are still under development, but have already given some promising results (e.g., has been used to measure large genetic variability in BNF within different NFTs). Various factors affect BNF in trees. They include the age of trees, the microbial component, soil moisture, temperature, salinity, pH, soil N level and plant nutrient deficiencies. Some of the factors, e.g. temperature, affect the symbiosis more than plant growth, and differences in the effects of these factors on BNF in different NFT genotypes have been reported. These factors and research needs for improving BNF in trees are discussed.
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  • 80
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    Plant and soil 142 (1992), S. 53-61 
    ISSN: 1573-5036
    Keywords: neem ; N fertilizer ; nitrate ; Oryza sativa L. ; Vigna unguiculata (L.) Walp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cowpea, Vigna unguiculata (L.) Walp., is well adapted to acid upland soil and can be grown for seed, green manure, and fodder production. A 2-yr field experiment was conducted on an Aeric Tropaqualf in the Philippines to determine the effect of cowpea management practice on the response of a subsequent upland rice crop to applied urea. Cowpea was grown to flowering and incorporated as a green manure or grown to maturity with either grain and pods removed or all aboveground vegetation removed before sowing rice. Cowpea green manure accumulated on average 68 kg N ha−1, and aboveground residue after harvest of dry pods contained on average 46 kg N ha−1. Compared with a pre-rice fallow, cowpea green manure and residue increased grain yield of upland rice by 0.7 Mg ha−1 when no urea was applied to rice. Green manure and residue substituted for 66 and 70 kg urea-N ha−1 on upland rice, respectively. In the absence of urea, green manure and residue increased total aboveground N in mature rice by 12 and 14 kg N ha−1, respectively. These increases corresponded to plant recoveries of 13% for applied green manure N and 24% for applied residue N. At 15 d after sowing rice (DAS), 33% of the added green manure N and 16% of the added residue N was recovered as soil (nitrate + ammonium)-N. At 30 DAS, the corresponding recoveries were 20 and 37% for green manure N and residue N, respectively. Cowpea cropping with removal of all aboveground cowpea vegetation slightly increased (p〈0.05) soil (nitrate + ammonium)-N at 15 DAS as compared with the pre-rice fallow, but it did not increase rice yield. Cowpea residue remaining after harvest of dry pods can be an effective N source for a subsequent upland rice crop.
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  • 81
    ISSN: 1573-5036
    Keywords: intercropping ; isotope dilution ; Lupinus angustifolius ; 15N ; nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen fixation was measured in monocropped sweet-blue lupin (Lupinus angustifolius), lupin intercropped with two ryegrass (Lolium multiflorum) cultivars or with oats (Avena sativa) on an Andosol soil, using the 15N isotope dilution method. At 117 days after planting and at a mean temperature below 10°C, monocropped lupin derived an average of 92% or 195 kg N ha−1 of its N from N2 fixation. Intercropping lupin with cereals increased (p〈0.05) the percentage of N derived from atmospheric N2 (% Ndfa) to a mean of 96%. Compared to the monocropped, total N fixed per hectare in intercropped lupin declined approximately 50%, in line with the decrease in seeding rate and dry matter yield. With these high values of N2 fixation, selection of the reference crop was not a problem; all the cereals, intercropped or grown singly produced similar estimates of N2 fixed in lupin. It was deduced from the 15N data that significant N transfer occurred from lupin to intercropped Italian ryegrass but not to intercropped Westerwoldian ryegrass or to oats. Doubling the 15N fertilizer rate from 30 to 60 kg N ha−1 decreased % Ndfa to 86% (p〈0.05), but total N fixed was unaltered. These results indicate that lupin has a high potential for N2 fixation at low temperatures, and can maintain higher rates of N2 fixation in soils of high N than many other forage and pasture legumes.
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  • 82
    ISSN: 1573-5036
    Keywords: ammonium ; carboxylates ; H+/OH- efflux ; ionic balance ; nitrate ; nitrate reductase activity ; nitrogen starvation ; Rumex acetosa ; Rumex crispus ; Rumex maritimus ; Rumex palustris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Growth, chemical composition, and nitrate reductase activity (NRA) of hydroponically cultured Rumex crispus, R. palustris, R. acetosa, and R. maritimus were studied in relation to form (NH4 +, NO3 -, or both) and level of N supply (4 mM N, and zero-N following a period of 4mM N). A distinct preference for either NH4 + or NO3 - could not be established. All species were characterized by a very efficient uptake and utilization of N, irrespective of N source, as evident from high concentrations of organic N in the tissues and concurrent excessive accumulations of free NO3 - and free NH4 +. Especially the accumulation of free NH4 + was unusually large. Generally, relative growth rate (RGR) was highest with a combination of NH4 + and NO3 -. Compared to mixed N supply, RGR of NO3 -- and NH4 +-grown plants declined on average 3% and 9%, respectively. Lowest RGR with NH4 + supply probably resulted from direct or indirect toxicity effects associated with high NH4 + and/or low Ca2+ contents of tissues. NRA in NO3 - and NH4NO3 plants was very similar with maxima in the leaves of ca 40 μmol NO2 - g-1 DW h-1. ‘Basal’ NRA levels in shoot tissues of NH4 + plants appeared relatively high with maxima in the leaves of ca 20 μmol NO2 - g-1 DW h-1. Carboxylate to organic N ratios, (C-A)/Norg, on a whole plant basis varied from 0.2 in NH4 + plants to 0.9 in NO3 - plants. After withdrawal of N, all accumulated NO3 - and NH4 + was assimilated into organic N and the organic N redistributed on a large scale. NRA rapidly declined to similar low levels, irrespective of previous N source. Shoot/root ratios of -N plants were 50–80% lower than those from +N plants. In comparison with +N, RGR of -N plants did not decline to a large extent, decreasing by only 15% in -NH4 + plants due to very high initial organic-N contents. N-deprived plants all exhibited an excess cation over anion uptake (net proton efflux), and whole-plant (C-A)/Norg ratios increased to values around unity. Possible difficulties in interpreting the (C-A)/Norg ratio and NRA of plants in their natural habitats are briefly discussed.
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  • 83
    ISSN: 1573-5036
    Keywords: asparagus ; Asparagus officinalis ; nitrogen fertilizer ; 15N ; plant uptake ; soil nitrogen fractions ; time of application
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of nitrogen (N) fertilizer on the production of a 6-year-old asparagus (Asparagus officinalis L.) crop was examined over 2 years by the application of 0, 50 or 100 kg N ha−1 as ammonium sulphate at three times; 1) prior to fern growth (9 months before harvest), 2) prior to harvest, or 3) early-harvest prior to the main period of spear production. The utilization of N fertilizer was examined by applying 15N-enriched ammonium sulphate to 2 m×2.5 m microplost within the 50 kg N ha−1 treatments. There was a 12% response in spear production to added N in the first harvest year only and there was no significant effect of rate or time of N application. Plant uptake of added 15N by the end of the first harvest period was 25, 11 and 4% of the total applied for the pre-fern, pre-harvest and early-harvest treatments respectively. About 60% of the 15N applied pre- or early-harvest remained in the soil at the end of the first harvest period. Most of the 15N in soil in the pre-harvest treatment occurred as inorganic N and had been leached into the 150–600 mm soil depth. In contrast, most of the 15N that remained in soil from the early-harvest application was in the 0–75 mm depth and 40% of this had been immobilized into organic N. In the short-term (less than one year), utilisation of 15N-labelled N fertilizer by asparagus was greatest when applied prior to fern growth. However, after 2 years there was no difference between treatments in 15N recovery in plant or soil. Thus, time of application of N fertilizer had no long-term effect on production or utilization of N fertilizer by asparagus.
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  • 84
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    Plant and soil 147 (1992), S. 59-68 
    ISSN: 1573-5036
    Keywords: kiwifruit ; 15N ; plant uptake ; remobilisation ; temporal changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Temporal changes in the nitrogen (N) and 15N content of various components of six-year-old kiwifruit (Actinidia deliciosa ‘Hayward’) vines which had received 15N-labelled ammonium fertilizer were measured. The fertilizer was applied singularly at 100 or 200 kg N ha−1 in early spring (two weeks before bud burst) or split with 100 kg N ha−1 (unlabelled) in early spring and 100 kg N ha−1 (15N-labelled) ten weeks later. All treatments were applied to vines with a history of either 50 or 200 kg N ha−1 yr−1. The N concentration of leaf and fruit tissue was generally lower in the 100 kg N ha−1 treatment than in the 200 kg N ha−1 treatments and this effect was greater than that of N fertilizer history. During the first 8 weeks after bud burst there was a rapid accumulation of N in leaves (ca. 80 kg N ha−1). Analysis of xylem sap at 4 weeks after bud burst revealed that about 60% of the N utilised for new growth was from remobilisation of N stored within the vines and about 40% from soil and fertilizer N. This was unaffected by rate of N application. Plant uptake of added 15N was rapid and almost complete within 10 weeks of application in either early spring or early summer. Initially, most 15N was present in the leaf and root components but these subsequently declined due to translocation into other components of the vine. The decline in leaf 15N coincided with an equivalent accumulation of 15N in the fruit. The 15N enrichment of the ‘annual’ components (leaves, fruit and current-season's shoots) was about twice that of the structural components (one-year-old shoots, cordon, stem and structural roots) during the first year after application. By the third year the 15N concentration of the annual components had declined to similar levels to that of the structural components. These changes were used to estimate the annual throughput of N from soil in vines which received no N fertilizer in years 2 and 3 at 50% of total above-ground N and only 7% in roots. This was equivalent to about 120 kg N ha−1 yr−1. Where fertilizer N (200 kg ha−1) was applied in year 2 the annual throughput of N from soil and fertilizer was about 170 kg N ha−1.
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  • 85
    ISSN: 1573-5036
    Keywords: alkaloid synthesis ; ammonium ; bound nitrogen ; Datura stramonium ; hyoscyamine ; mineral nutrition ; nitrate ; scopolamine ; vermiculite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plants of Datura stramonium var. tatula L. Torr. were cultivated on vermiculite and received two different mineral solutions. In one treatment only NO3 −-nitrogen was added, while in the other NO3 −-nitrogen was partly (20%) replaced by NH4 +-nitrogen. Total dose of nitrogen as well as interionic ratios were kept constant in both treatments. With the combined treatment (NO3 −-NH4 +) a significant higher hyoscyamine content was found at the time when highest biomass was reached. This was apparently the result of an increased alkaloid biosynthesis. Also scopolamine content was positively influenced, but only at a point past maximal biomass yield. No significant differences in amounts of nitrogen bound per plant were found between both treatments. The higher alkaloid content observed with the combined treatment was associated with a higher relative proportion of bound nitrogen present in the alkaloids. It seems that more nitrogen is available for secondary metabolism when NH4 +-nitrogen is present in the culture medium.
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  • 86
    ISSN: 1573-5036
    Keywords: fertilizer ; genotype ; isotope dilution ; legume ; 15N ; nitrogen fixation ; nodule ; phosphorus use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A promising approach for overcoming poor crop yields in phosphorus (P)-deficient soils is to exploit the genetic variation among plants to grow under low P conditions. We examined the P requirements of three mungbean cultivars, T-77, MI-5 and E-72, using four P rates, 0, 30, 60 and 90 mg P kg-1 soil (designated P0, P1, P2 and P3, respectively). Nodulation was highest in T-77, and unlike the other cultivars, nodule numbers were not increased by P application. Similarly, growth of T-77 was the highest, and was not influenced by P rates. In contrast shoot yields of MI-5 and E-72 at P0 were only 76 and 65%, respectively, of the maximum obtained under P application. Nodule dry weight and the amount of N fixed (Ndfa) in each cultivar was enhanced by P application, with T-77 generally giving the lowest response, and accumulating the highest Ndfa. The data suggest a higher P requirement for N2 fixation (especially for T-77) than for growth. All plants increased their P uptake as P rates increased, with T-77 accumulating the highest amount of P at each P level. Differences in the physiological P use efficiency, PPUE (g shoot mg-1 P) among genotypes were generally not significant, neither were there any consistent trends as P rates changed. The ability to absorb P therefore appeared to be more important than PPUE in enhancing growth. We conclude from our data that it is possible by selection to obtain plants capable of good growth and high N2 fixation in soils of low P; cultivar T-77 is a good example.
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  • 87
    ISSN: 1573-0867
    Keywords: Ammonium ; cabbage ; nitrate ; nitrogen fertilizer ; nitrification inhibitor ; organic fertilizer ; soil microbial activity ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Yield response of summer cabbage (Brassica oleracea varcapitata cv. Hispi F1) to N applied as organic (activated sewage sludge derived protein [Protox] and dried blood) and inorganic (ammonium nitrate, ammonium sulphate, sodium nitrate and urea) fertilizers was compared in relation to the N availability characteristics of the materials. Effects of the nitrification inhibitor dicyandiamide (DCD) on N release, crop yield and N status were also assessed. In addition CO2 efflux was measured from amended soil to determine effects of fertilizer application on soil microbial activity. The organic N sources were mineralized quickly on application to soil and exhibited similar patterns of NH4-N depletion and NO3-N accumulation as functions of thermal-time as with mineral fertilizers. However, the yield response to organic N was marginally smaller (though not significantly) compared with mineral forms; probably because less N was released to the crop. This was reflected in smaller total N concentrations and N recoveries in plants supplied with organic fertilizer. Applied DCD increased the thermal-time for complete nitrification of NH4-N sources and raised the total N content of the crop, but had no overall effect on crop growth. In contrast to inorganic N sources which generally reduced CO2 efflux from soil, application of protein-based fertilizers increased the rate of soil microbial activity directly by raising substrate availability. Sewage sludge derived protein provided an effective alternative to mineral fertilizers for the nutrition of summer cabbage whilst minimising stress of the soil environment which may occur following the application of conventional forms of inorganic N to the soil.
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  • 88
    ISSN: 1573-0867
    Keywords: Cultivation ; leaching ; mineralization ; mixed cropping ; nitrate ; nitrogen ; pasture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted to investigate the effect of timing and method of cultivation of a 3-year old ryegrass/white clover pasture on subsequent N mineralization, NO 3 - -N leaching, and growth and N uptake of a wheat crop in the following season. The size of various N pools and decomposition of14C-labelled ryegrass material were also investigated. Cultivation method (mouldboard or chisel ploughing) generally had no significant effect on the accumulation of mineral N in the profile in the autumn or on the amount of NO 3 - -N leached over winter.14C measurements suggested that initial decomposition rate of plant material was faster from May than March cultivation treatments. Despite this, overall net mineralization of organic N (of soil plus plant origin) increased with increasing fallow period between cultivation and leaching. The total amounts of mineral N accumulated in the soil profile before the start of leaching were 139, 119 and 22 kg N ha−1 for the March, May and July cultivated soils respectively. Cumulative leaching losses over the trial calculated from soil solution samples were 78, 40 and 5 kg N ha−1 for the March, May and July cultivated soils respectively. Differences in N mineralization over the season were generally not reflected by changes in amounts of potentially-mineralizable soil N (as measured by extraction or laboratory incubation) or levels of microbial biomass during the season. The amount of mineral N in the profile in spring increased with decreasing fallow period. This was reflected in an approximately 15% and 25% greater grain yield and N uptake respectively by the following wheat crop in plots cultivated in July rather than in March.
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  • 89
    ISSN: 1573-5036
    Keywords: 13C ; carbon isotope discrimination ; 15N ; % Ndfa ; N2 fixation ; salinity ; soybeans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A preliminary study was conducted on greenhouse-grown soybean plants to investigate if carbon isotope (13C) discrimination (Δ) is related with the capacity to fix atmospheric nitrogen (N2). Seven soybean (Glycine max (L.) Merrill) cultivars (Goldsoy, A-100, Lee, Ada, D61-2694, Alto and Evans) inoculated with a mixture of Bradyrhizobium japonicum were tested. A salinity treatment imposed on one set of plants increased the range in Δ (18.72 × 103 − 19.56 × 103 without salinity to 18.72 × 103 − 20.36 × 103 with salinity) and N2 fixation (79.4%–84.9% without salinity to 73.6%–84.9% with salinity) attainable in the seven cultivars studied. For the full range of treatments, Δ was negatively correlated (r=−0.62*) with N2 fixation. Thus, subject to confirmation from further study, Δ may prove a useful tool in the selection of legume genotypes with a high capacity for N2 fixation.
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  • 90
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    Plant and soil 144 (1992), S. 85-92 
    ISSN: 1573-5036
    Keywords: beans (Phaseolus vulgaris L.) ; Costa Rica ; dystrandept ; intercropping ; maize (Zea mays L.) ; N transfer ; 15N ; VAM fungi (Glomus etunicatum)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen (N) transfer from N-fixing legumes via vesicular-arbuscular mycorrhizal (VAM) fungi to associated non-fixing plants has been demonstrated in greenhouse experiments. To date, this transfer has been shown only where mineral N is applied shortly before harvest, and hence is readily available. We have yet to demonstrate VAM-mediated N transfer where soil-N is limiting, a condition under which most traditional legume-nonlegume intercrops are grown. In this study, 15N-enriched soil (with 0.28%N) was used to distinguish between the uptake of soil- and atmospherically-derived N in maize grown with beans in the presence or absence of VAM fungi. VAM infection did not result in transfer of fixed N or soil N from bean to maize, despite a VAM-stimulated increase in N fixation in bean. In fact, beans were more competitive for soil N when mycorrhizal. N content in beans increased by 75% with a concomitant 22% decrease in mg N per maize plant. The competitive effect may have resulted from a VAM-mediated shift in carbon allocation in beans (but not maize) from shoots to roots.
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  • 91
    ISSN: 1573-5036
    Keywords: isotope dilution ; lupins ; 15N ; 35S ; symbiotic N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lupins, canola, ryegrass and wheat fertilized with Na2 35SO4 and either 15NH4Cl or K15NO3(N:S=10:1), were grown in the field in unconfined microplots, and the sources of N and S (fertilizer, soil, atmosphere, seed) in plant tops during crop development were estimated. Modelled estimates of the proportion of lupin N derived from the atmosphere, which were obtained independently of reference plants, were used to calculate the proportion of lupin N derived from the soil. Total uptake of N and S and uptake of labelled N and S increased during crop development. Total uptake of S by canola was higher than lupins, but labelled S uptake by lupins exceeded uptake by canola. The form of N applied had no effect on uptake of labelled and unlabelled forms of N or S. Ratios of labelled to unlabelled S and ratios of labelled to unlabelled N derived from soil sources decreased during growth, and were less for S than for N for each crop at each sampling time. Although ratios of labelled to unlabelled soil-derived N were similar between crops at 155, 176 and 190 days after sowing, ratios of labelled to unlabelled S for lupins were higher than for the reference crops and declined during this period. The ratios of labelled to unlabelled S in lupins and the reference plants therefore bore no relationship either to ratios of labelled to unlabelled soil-derived N in the plants, or to total S uptake by the plants. Therefore the hypothesis that equal ratios of labelled N to unlabelled soil-derived N in legumes (Rleg) and reference plants (Rref) would be indicated by equal ratios of labelled to unlabelled S was not supported by the data. The results therefore show that the accuracy of reference plant-derived values of Rleg cannot be evaluated by labelling with 35S.
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  • 92
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    Nutrient cycling in agroecosystems 29 (1991), S. 309-316 
    ISSN: 1573-0867
    Keywords: Lolium perenne ; Coron ; N-Sure ; Nitrazine ; melamine ; urea ; nitrate ; N metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The absorption of three new slow-release fertilizers (Coron, N-Sure and Nitrazine) by perennial ryegrass turf was compared to uptake of NO 3 - and urea using a nutrient solution culture system. Each source of nitrogen was supplied to turf cultures at a rate equivalent to 21 kg N ha−1 every five days during a twenty day experimental period. Nitrate and urea produced the most growth, while growth on Coron and N-Sure was reduced 30%. Growth on Nitrazine-N was further reduced to only 40% of that on NO 3 - and urea. Coron and N-Sure were absorbed relatively rapidly during the first 24 hour period, with cumulative absorption over the five day period amounting to approximately 80% of the total supplied. Nitrazine-N was absorbed more slowly, with only 40% of the N absorbed after five days. Perennial ryegrass was apparently capable of metabolizing both Coron and N-Sure. The slow-release N component of Nitrazine (melamine) was inhibitory to photosynthesis, and at higher solution concentrations, was toxic to the turf.
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  • 93
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    Biology and fertility of soils 11 (1991), S. 97-100 
    ISSN: 1432-0789
    Keywords: Added N interaction ; Fertilizer N ; 15N ; Organic amendment ; Oryza sativa ; Wetland rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A pot experiment was conducted to study the availability of soil and fertilizer N to wetland rice as influenced by wheat straw amendment (organic amendment) and to establish the relative significance of the two sources in affecting crop yield. Straw was incorporated in soil at 0.1, 0.2, and 0.3% before transplanting rice. Inorganic N as 15N-ammonium sulphate was applied at 30, 60, and 90 μg g-1 soil either alone or together with wheat straw in different combinations. After harvesting the rice, the plant and soil samples were analyzed for total N and 15N. Straw incorporation significantly decreased the dry matter and N yield of rice, the decrease being greater with higher rates of straw. The reduction in crop yield following the straw incorporation was attributed mainly to a decrease in the uptake of soil N rather than fertilizer N. The harmful effects of organic matter amendment were mitigated by higher levels of mineral N addition. The uptake of applied N increased and its losses decreased due to the straw incorporation. Mineral N applied alone or together with organic amendment substantially increased the uptake of unlabelled soil N. The increase was attributed to a real added N interaction.
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  • 94
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    Biology and fertility of soils 11 (1991), S. 116-120 
    ISSN: 1432-0789
    Keywords: Denitrification ; Flooded soil ; 15N ; Urea ; Wetland rice ; Oryza sativa L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The efficiency of N use in flooded rice is usually low, chiefly due to gaseous losses. Emission of CH4, a gas implicated in global warming, can also be substantial in flooded rice. In a greenhouse study, the nitrification inhibitor encapsulated calcium carbide (a slow-release source of acetylene) was added with 75, 150, and 225 mg of 75 atom % 15N urea-N to flooded pots containing 18-day-old rice (Oryza sativa L.) plants. Urea treatments without calcium carbide were included as controls. After the application of encapsulated calcium carbide, 3.6 μg N2, 12.4 μg N2O-N, and 3.6 mg CH4 were emitted per pot in 30 days. Without calcium carbide, 3.0 mg N2, 22.8 μg N2O-N, and 39.0 mg CH4 per pot were emitted during the same period. The rate of N added had a positive effect on N2 and N2O emissions, but the effect on CH4 emissions varied with time. Carbon dioxide emissions were lower with encapsulated calcium carbide than without. The use of encapsulated calcium carbide appears effective in eliminating N2 losses, and in minimizing emissions of the “greenhouse gases” N2O and CH4 in flooded rice.
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  • 95
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    Biology and fertility of soils 12 (1991), S. 10-18 
    ISSN: 1432-0789
    Keywords: Agroecological rotation ; Hordeum vulgare ; Microbial biomass ; 15N ; Rotation effects ; Pot experiment ; Soil nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soil N dynamics and barley yields (Hordeum vulgare L.) were compared in pot experiments using surface samples from a Gray Luvisol under three cropping systems at Breton, Alberta: (1) an agroecological 8-year rotation including cereals, forage, and fababeans (Vicia faba L.) as green manure, from wich two plots were selected, one following fababeans, and the second following 3 years of forage; (2) a continuous grain system, with fertilizer N at 90 kg ha-1 year-1; and (3) a classical Breton 5-year rotation [following oats (Avena sativa L.)] involving forage and cereals, without returning crop residues to the land, selecting one plot with PKS treatment and a second as control. The fertilizer N equivalent for the cropping system; “AN” value and “A” value (analogous to AN), but in fertilizer 15N units, soil biomass, and C and N mineralization were monitored. In the first agroecological plot (after fababeans), grain and total plant biomass production were 116% greater than from the continuous grain treatment. Barley plants in the two agroecological plots derived 48.5% and 37.8%, respectively, of their N requirement from non-labelled soil N sources not present in the continuous grain plot. At crop maturity, the recovery of 15N microbial biomass was 1.5 times higher in soil from the first agroecological plot than from the continuous grain plot. The fertilizer N equivalent was 2670 mg pot-1 (485 kg ha-1) for the first and 1850 mg pot-1 for the second agroecological treatment. Fertilizer N equivalent values exceed net amounts of N mineralized by a factor of 4. Recovery by the barley crop of 15N added at 55 mg pot-1 was more efficient in the agroecological treatments (45%–51%) than in the continuous grain or classical Breton treatments (35%–37%). It was concluded (1) that past soil history may be associated more with the ability of barley plants to compete for available N, and hence the use of N, than with net soil N mineralization; and (2) an increased supply of N to crops following the incorporation of fababean residues, manure application, and the soil N-conserving effect of growing legumes were all partly responsible for the observed differences in soil fertility.
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  • 96
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    Plant and soil 131 (1991), S. 89-96 
    ISSN: 1573-5036
    Keywords: ammonium ; mineralization ; nitrate ; nitrification ; 15N ; plant ; soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Details are presented of a simple mathematical framework that allows 15N tracer experiments to be interpreted in terms of the main processes of the soil/plant nitrogen cycle. The calculations, all of which can be performed on a scientific calculator, yield the rates of gross mineralization and nitrification and the crop nitrogen uptake occurring as ammonium and nitrate. Two procedures are presented. One requires paired experiments with labelled ammonium and unlabelled nitrate as one treatment, and unlabelled ammonium and labelled nitrate as the other. The second procedure requires only the labelled ammonium, unlabelled nitrate treatment. Example calculations are presented using actual experimental data. The interpretative procedure uses the fact that the rate of isotopic dilution in an ammonium pool labelled with 15N is a function of the rate at which unlabelled ammonium is introduced into the pool via mineralization. Similarly, the rate of isotope dilution in an 15N labelled nitrate pool is a function of the rate at which unlabelled nitrate is introduced into the pool via nitrification.
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  • 97
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    Plant and soil 131 (1991), S. 97-105 
    ISSN: 1573-5036
    Keywords: ammonium ; mineralization ; nitrate ; nitrification ; 15N ; plant ; soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A pulse dilution 15N technique was used in the field to determine the effect of the ammonium to nitrate ratio in a fertilizer application on the uptake of ammonium and nitrate by ryegrass and on gross rates of mineralization and nitrification. Two experiments were performed, corresponding approximately to the first and second cuts of grass. Where no substantial recent immobilization of inorganic nitrogen had occurred, mineralization was insensitive to the form of nitrogen applied, ranging from 2.1–2.6 kg N ha-1 d-1. The immobilization of ammonium increased as the proportion of ammonium in the application increased. In the second experiment there was evidence that high rates of immobilization in the first experiment were associated with high rates of mineralization in the second. The implication was that some nitrogen immobilized in the first experiment was re-mineralized during the second. Whether this was nitrogen taken up, stored in roots and released following defoliation was not clear. Nitrification rates in this soil were low (0.1–0.63 kg N ha-1 d-1), and as a result, varying the ratio of ammonium to nitrate applied markedly altered the relative uptake of ammonium and nitrate. In the first experiment, where temperatures were low, preferential uptake of ammonium occurred, but where 〉90% of the uptake was as ammonium, a reduction in yield and nitrogen uptake was observed. In the second experiment, where temperatures and growth rates were higher, the proportion of ammonium to nitrate taken up had no effect on yield or nitrogen uptake.
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  • 98
    ISSN: 1573-5036
    Keywords: lucerne ; nitrate ; nitrate reductase ; Rhizobium meliloti
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen fixing plants of lucerne (Medicago sativa L. cv. Aragón) were grown in a glasshouse for three months in the absence of nitrate, and then supplied with 5 mM KNO3 for a week. In control (non-nitrate fed) plants, nitrate reductase activity (NRA EC 1.6.6.1) was detected only in nodules. After nitrate supply, root NRA showed a transient increase. Shoot NRA increased with time, paralleling changes in nitrate distribution; stem NRA represented nearly 50% of total NRA in plant tissues. Total nitrogen, expressed on a dry weight basis, tended to decrease in shoots upon nitrate supply. Bacteroid NRA (EC 1.7.99.4) showed a great variation depending on Rhizobium meliloti strains, ranging from 5 to 40% of total plant NRA. However, different Rhizobium strains did not give different results in terms of plant growth parameters, nitrate or organic nitrogen content.
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  • 99
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    Plant and soil 132 (1991), S. 29-39 
    ISSN: 1573-5036
    Keywords: actinorhizal plants ; associative nitrogen fixation ; cereals ; grasses ; isotope dilution ; 15N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract During the past 10 years estimates of N2 fixation associated with sugar cane, forage grasses, cereals and actinorhizal plants grown in soil with and without addition of inoculum have been obtained using the 15N isotope dilution technique. These experiments are reviewed in this paper with the aim of determining the proportional and absolute contribution of N2 fixation to the N nutrition of non-legumes, and its role as a source of N in agriculture. The review also identifies deficiencies in both the totality of data which are currently available and the experimental approaches used to quantify N2 fixation associated with non-legumes. Field data indicate that associative N2 fixation can potentially contribute agronomically-significant amounts of N (〉30–40 kg N ha-1 y-1) to the N nutrition of plants of importance in tropical agriculture, including sugar cane (Saccharum sp.) and forage grasses (Panicum maximum, Brachiaria sp. and Leptochloa fusca) when grown in uninoculated, N-deficient soils. Marked variations in proportions of plant N derived from the atmosphere have been measured between species or cultivars within species. Limited pot-culture data indicate that rice can benefit naturally from associative N2 fixation, and that inoculation responses due to N2 fixation can occur. Wheat can also respond to inoculation but responses do not appear to be due to associative N2 fixation. 15N dilution studies confirm that substantial amounts of N2 can be fixed by actinorhizal plants.
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  • 100
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    Plant and soil 133 (1991), S. 47-56 
    ISSN: 1573-5036
    Keywords: clover ; competition ; grass ; Lolium perenne ; 15N ; nitrogen fixation ; Phleum pratense ; temperature ; Trifolium repens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract It was the aim of this study to determine the way in which low temperature modifies the effect of a competing grass on nitrogen fixation of a forage legume. White clover (Trifolium repens L.) was grown in monoculture or in different planting ratios with timothy (Phleum pratense L.) or perennial ryegress (Lolium perenne L.) in growth chambers at either 7.5/5°C (LoT) or 15/10°C (HiT) average day/night temperatures, and with 2.5 or 7.5 mM 15N-labelled nitrate in the nutrient solution. Competition with grass led to a marked increase in the proportion of clover nitrogen derived from symbiosis (% Nsym). This increase was slower at LoT where % Nsym was reduced considerably; it was closely related to the reduction in the amount of available nitrate as a result of its being utilized by the grass. Nitrogen concentration in white clover herbage and dry matter yield per clover plant were reduced, for the most part, when a competing grass was present. The amount of nitrogen fixed per plant of white clover decreased markedly with temperature. Low temperature consequently accentuated competition for nitrate. The capacity of white clover to compete successfully was limited by its slower growth and nitrogen accumulation.
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