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  • 1
    ISSN: 1432-0789
    Keywords: Key words Net mineralization ; Nitrification ; Denitrification ; Leaching ; Field incubation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An improved method is described for incubating intact soil cores in the field, which permits concurrent measurement of net mineralization, nitrification, denitrification and leaching. Cores were enclosed in PVC tubes with minimal disturbance to the physical state or to the natural cycles of wetting/drying, soil temperature and aeration during an incubation lasting 4–5 days. An example of the application of the method is given in which soils with contrasting drainage characteristics were compared. Over a 64-day experimental period, 58% of the mineralized nitrogen (N) in a freely drained soil was nitrified and 36% of the nitrate-N (NO3 –-N) was denitrified. In a poorly drained soil, 72% of the mineralized N was nitrified and 63% of the NO3 –-N was denitrified. In both soil types, 18% of the remaining NO3 –-N was leached. Rates of nitrification were significantly correlated with net mineralization (r 2=0.41 and 0.52) and also closely correlated with denitrification (r 2=0.67 and 0.68) in the freely and poorly drained soils, respectively. Independent measurements of these processes, using alternative techniques (for the same period), compared favourably with measurements obtained with the improved incubation method. Adoption of this method has a number of advantages with respect to field net N mineralization, and also allows interpretation of the impact this may have on other N transformation processes.
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  • 2
    ISSN: 1432-0789
    Keywords: Key words Nitrogen-15 natural abundance variability ; Plant tissues ; Grassland ; Sward management ; Dinitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The 15N natural abundance (δ15N) of white clover (Trifolium repens L.) grown in pasture under different management practices was determined. Plants were split into leaflets, petioles and stolons and the 15N signature of each tissue was measured. The δ15N of leaflet tissue from plants of two non-N2-fixing species (Lolium perenne L. and Ranunculus repens L.), growing in close proximity to the sampled T. repens, was also measured. By using T. repens plants grown in the absence of mineral N to provide reference material, the proportion of N derived from N2 fixation (%Ndfa) in pasture plants was calculated. Within a plot, variation was present in the δ15N between the tissues of T. repens. Variation was also present between the same tissues under different management practices. The %Ndfa in the leaf material of T. repens varied from 34% to 100% between the plots. The use of different reference species did not affect the estimate of %Ndfa.
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  • 3
    ISSN: 1432-0789
    Keywords: Key words Soluble organic nitrogen ; Mineral nitrogen ; Potentially mineralisable nitrogen ; Cultivation ; Grassland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  This study evaluated the effect of cultivation and reseeding on the distribution and fate of soil mineral N (SMN), soluble organic N (SON) and potentially mineralisable N (PMN) in the soil profile of two long-term grasslands in the UK. Cultivation and reseeding significantly increased the total soluble N concentration (SMN plus SON) of the soil profile (0–90 cm), with over 50 mg SON kg–1 observed. By contrast, the PMN pool was unaffected by cultivation and declined with increasing soil depth. The flush in SON and SMN observed in both soils disappeared within 1 year following cultivation. The fate of SON appeared to be dependent on soil type, with considerably more movement to deeper layers apparent in the profile of a silty clay loam (30% clay) than in a clay loam (49% clay). Mineralisation and/or immobilisation of SON in the topsoil probably accounted for the changes observed in the SON content of the clay loam. SON is an important N pool in grassland soils and cultivation has a significant impact on its release. Measurements of SON should therefore be included in studies of N cycling in agricultural cropping systems, so that full account may be taken of its potential as a source or sink of mobile N.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 29 (1999), S. 38-45 
    ISSN: 1432-0789
    Keywords: Key words Grassland ; Nitrogen ; Mineralization ; Macro-organic matter ; Soil particles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  A study was conducted to determine mineralization rates in the field and in different soil layers under three grassland managements (viz. a reseeded sward, a permanent sward with a conventional N management, and a long-term grass sward with 0 N (0-N) input). Potential mineralization rates of soil particles (sand, silt and clay) and macro-organic matter fractions of different sizes (i.e. 0.2–0.5, 0.5–2.0 and 〉2 mm) were also determined in the laboratory. In the reseeded plots, net mineralization was unchanged down to 40 cm depth. In the undisturbed conventional-N swards, mineralization rates were substantially higher in the top layer (0–10 cm) than in the deeper layers. In plots which had received no fertilizer N, mineralization was consistently low in all the layers. There was more macro-organic matter (MOM) in the 0-N plots (equivalent to 23 g kg–1 soil for 0–40 cm) than in the two fertilized plots (i.e. conventional-N and reseeded) which contained similar amounts (ca. 15 g kg–1 soil). C and N contents of separated soil particles did not differ amongst the treatments, but there were large differences with depth. Potential mineralization in the bulk soil was greatest in the 0–10 cm layers and gradually decreased with depth in all the treatments. Separated sand particles had negligible rates of potential mineralization and the clay component had the highest rates in the subsurface layers (10–40 cm). MOMs had high potential rate of mineralization in the surface layer and decreased with soil depth, but there was no clear pattern in the differences between different size fractions.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 24 (1997), S. 147-152 
    ISSN: 1432-0789
    Keywords: Key words Grassland soils ; Humic materials ; δ15N ; Nitrogen cycling ; Soil organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The natural abundance of δ15N in disturbed and undisturbed pasture soils was examined. From the disturbed soil, the top 10 cm of the profile was examined and the soil split into fractions based on particle size. Plant shoot and root material contained similar low enrichments in 15N, whereas recently deposited shoot residues were highly enriched. Differences between the soil fractions in observed total N did not reflect similar 15N variation. However, the enrichment of humic material extracted from the largest soil fraction was considerably lower in 15N relative to that from the smaller fractions. The complexity of the humic material from the larger fractions was less according to the E 4 /E 6 ratio. Analysis of the profile from the undisturbed soil showed increasing 15N enrichment with depth which corresponded well with visible soil horizons and showed an inverse relationship with total soil N. This 15N enrichment was mirrored by the enrichment in humic materials down the profile and also corresponded with an increasing chemical complexity as shown by the E 4 /E 6 ratio.
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  • 6
    ISSN: 1432-0789
    Keywords: Key words Nitrogen-15 mean pool dilution ; Mineralization ; Immobilization ; Nitrification ; Field incubation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The 15N isotope dilution method was combined with a field incubation technique to provide simultaneous measurements of gross and net rates of N turnover in three long-term swards: unfertilized (Z) or receiving N either from N fixation as clover (C), or as 200 kg fertilizer N ha–1 year–1 (F). Uniform N enrichment of soil microplots was achieved with a multi-point soil injector to measure mineralization/immobilization turnover and nitrification over a 4-day incubation. Net rates of mineralization ranged between 0.6 and 2.9 μg N g–1 day–1 and in all three treatments were approximately half the gross rates. Nitrification rates (gross) were between 1.0 and 1.6 μg N g–1 day–1. In the F treatment, the turnover of NH4 +-N and NO3 –-N pools was on a 2- and 4-day cycle, respectively, whereas in the N-limited treatments (C and Z) turnover rates were faster, with the NO3 –-N pools turning over twice as fast as the NH4 +-N pools. Therefore, available N was recycled more efficiently in the C and Z treatments, whereas in the F treatment a higher N pool size was maintained which would be more vulnerable to leakage. A large proportion of the added 15N was recovered in the soil microbial biomass (SMB), which represented a 4–5 times larger sink for N than the plant biomass. Although the C treatment had a significantly lower SMB than the grass-only treatments, there were no differences in microbial activity. Gross rates of nitrification increased along the gradient of N input intensity (i.e. Z〈C〈F), and the addition of a nitrification inhibitor (C2H2) tended to increase microbial immobilization, but did not influence plant N uptake. In this study, the value of combining different techniques to verify net rates was demonstrated and the improved methodology for 15N labelling of soil enabled measurements to be obtained from relatively undisturbed soil under natural field conditions.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 14 (1998), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Nitrogen (N) is of enviromental concern if it leaches or is released as nitrous oxide (N2O,). In order to utilize N efficiently in grazed pasture systems, the fluxes of N from various sources need to be quantified. One flux is N mineralization from organic sources. Previous work has examined incubation and chemical extraction of soils as methods to determine N mineralization potential. This paper re-examines new and previously published data on net mineralization, with the aim of examining the relationships between soil thermal units, net N mineralization (measured using acetylene incubations) and dry matter production in pastures. Net N mineralization is expressed as N turnover (net N mineralization as a % of total soil N). Relationships are developed between soil thermal units, dry matter production, and N turnover. These relationships have potential in advising farmers on potential N mineralization from soil organic matter. A second use of such relationships is the modelling of N transformations in pasture systems. Further work should explore the effect of soil moisture on such relationships and examine the relationship between soil thermal units and uptake of N by pasture.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 13 (1997), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Ceramic suction cups were used to obtain samples of soil solution from permanently grazed swards receiving 200 kg N/ha/y. The suction cups were installed in 1 ha plots at 10, 30 and 60 an depth in a poorly drained, heavy clay soil in S. W. England. The plots were hydrologically isolated from each other by perimeter drains which channelled surface runoff water into v-notch weirs. In one treatment, artificial drainage by a system of field and mole drains also converged to outfalls through v-notch weirs, which enabled samples to be taken. Nitrate and a range of other ionic constituents were examined over a 12 month period in soil solutions taken from the suction cups and compared with leachate obtained from the field drains and surface channels. Field drain samples frequently exceeded the EC limit of 11.3 mg nitrate-N/1, but concentrations in suction cups obtained during the same period did not, and were up to ten-fold less. Although correlations for ions were found between different sampling depths and drainage samples, no clear patterns emerged. It was concluded that suction cups were inappropriate for the determination of the overall leaching losses in this soil type, but provided useful data on changes in ionic concentrations which occurred in different soil horizons through to drainage outfalls.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 14 (1998), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Phosphorus budgets have been compiled for two contrasting grassland farming systems in the UK; intensive dairy farming and extensive hill sheep production. Balance sheets of inputs, recycling of P through the soil–plant–animal pathway and outputs are presented to determine the potential rate of P accumulation in the two systems. A typical 57 ha intensive dairy farm with 129 lactating cows imports 2.48t P via fertilizer, bedding and concentrates, plus a small amount from the atmosphere, of which 0.98t are exported in milk, calves and transfer from soil to the aquatic environment. Therefore 1.5t of P are retained within the farm each year, which equates to an accumulation rate of 26 kg/ha in the plant-soil system. This surplus occurs despite a fertilizer input of only 16 kg/ha. However, a large proportion of P, equivalent to 27 kg/ha, is imported in feed concentrates.For the typical 841 ha hill sheep farm supporting 694 Blackface ewes, P inputs and outputs are 0.66 and 0.42 t/yr, respectively. Therefore, approximately 0.24t P are retained within the farm, which is equivalent to an accumulation rate of 0.28 kg/ha per yr. In comparison to the small annual inputs and outputs of P on the hill farm, much P, 2.48t, is recycled through the plant-soil and plant-animal-soil pathways on the hill sheep farm. For both farming systems there is a net input of P, although the rate of accumulation is ten times greater for the dairy farm where the annual retention of P represents 60% of the total P inputs, compared with 36% on the hill sheep farm.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 9 (1993), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The concern over leakage of nitrate into waters and loss of other forms of N to the environment demands an appraisal of N flows within complete systems. The grassland N cycle is complex, with interactive controls over fluxes and transformations, and has the potential for considerable losses. Although there are data from experimental systems, a total comprehension of flows is not yet possible.Intensive dairy farming has a number of opportunities for leaks. A ‘model’ system in SW England has an annual input of 25.6 tonnes of N: of this only 20% is transferred into protein or milk, a further 46% is lost to the wider environment, 34% is as yet unaccounted for and much is recycled. Recent research has provided new techniques to decrease losses. To meet the joint requirements of production and environmental concerns we need to consider N flows and supplies on an integrated, whole farm basis, and to take better account of mineral N in the soil profile in relation to current crop demand, local climate and past sward management.
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