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  • Articles  (1,419)
  • Chemistry  (1,385)
  • Fisheries
  • General Chemistry
  • SPACE SCIENCES
  • 2005-2009  (42)
  • 1995-1999  (1,377)
  • Medicine  (1,419)
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  • 1
    Publication Date: 2009-07-03
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Diederich, Francois -- England -- Nature. 2009 Jul 2;460(7251):33. doi: 10.1038/460033c.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19571863" target="_blank"〉PubMed〈/a〉
    Keywords: Chemistry ; Internet ; Periodicals as Topic/*standards/*trends ; Printing/*trends ; Societies, Scientific
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 2006-08-05
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Crowder, L B -- Osherenko, G -- Young, O R -- Airame, S -- Norse, E A -- Baron, N -- Day, J C -- Douvere, F -- Ehler, C N -- Halpern, B S -- Langdon, S J -- McLeod, K L -- Ogden, J C -- Peach, R E -- Rosenberg, A A -- Wilson, J A -- New York, N.Y. -- Science. 2006 Aug 4;313(5787):617-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Marine Conservation, Nicholas School of the Environment and Earth Sciences, Duke University Marine Laboratory, Beaufort, NC 28516, USA. lcrowder@duke.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16888124" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biodiversity ; *Conservation of Natural Resources ; *Ecosystem ; Environment ; Fisheries ; Fishes ; *Government Regulation ; *Marine Biology ; Oceans and Seas ; Population Dynamics ; Seawater ; United States
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2006-07-22
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Szathmary, Eors -- New York, N.Y. -- Science. 2006 Jul 21;313(5785):306-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Biology, Eotvos University Budapest, and Collegium Budapest (Institute for Advanced Study), 2 Szentharomsag utca, H-1014 Budapest, Hungary. szathmary@colbud.hu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16857926" target="_blank"〉PubMed〈/a〉
    Keywords: *Biological Evolution ; Chemical Phenomena ; Chemistry ; Computational Biology ; Cooperative Behavior ; Cultural Evolution ; Exobiology ; Humans ; Language ; Models, Biological ; Models, Theoretical ; Molecular Biology ; Origin of Life ; *Research ; Selection, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    Publication Date: 2006-04-29
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chapman, Demian D F -- Pikitch, Ellen K -- Babcock, Elizabeth A -- New York, N.Y. -- Science. 2006 Apr 28;312(5773):526-8; author reply 526-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16645076" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Anthozoa ; Biomass ; Caribbean Region ; *Conservation of Natural Resources ; *Ecosystem ; Fisheries ; *Fishes ; Predatory Behavior ; *Sharks
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2006-11-25
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enserink, Martin -- New York, N.Y. -- Science. 2006 Nov 24;314(5803):1235-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17124302" target="_blank"〉PubMed〈/a〉
    Keywords: Chemistry ; *Cooking ; *Food ; France ; History, 20th Century ; History, 21st Century
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2006-08-12
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Holden, Constance -- New York, N.Y. -- Science. 2006 Aug 11;313(5788):777.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16902121" target="_blank"〉PubMed〈/a〉
    Keywords: *Acoustics ; *Animal Identification Systems ; Animals ; *Ecosystem ; Environment ; Fisheries ; *Fishes ; International Cooperation ; Movement ; Oceans and Seas ; Seawater ; Telemetry
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2007-04-21
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Duarte, Carlos M -- Marba, Nuria -- Holmer, Marianne -- New York, N.Y. -- Science. 2007 Apr 20;316(5823):382-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉IMEDEA (CSIC-UIB), Instituto Mediterraneo de Estudios Avanzados, 07190 Esporles, Mallorca, Spain. carlosduarte@imedea.uib.es〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17446380" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Animals, Domestic ; *Aquaculture/statistics & numerical data/trends ; Biodiversity ; Ecosystem ; Fisheries ; Fishes ; *Food ; Invertebrates ; Oceans and Seas ; Plants ; Seawater ; Vertebrates
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
    Publication Date: 2008-02-16
    Description: The management and conservation of the world's oceans require synthesis of spatial data on the distribution and intensity of human activities and the overlap of their impacts on marine ecosystems. We developed an ecosystem-specific, multiscale spatial model to synthesize 17 global data sets of anthropogenic drivers of ecological change for 20 marine ecosystems. Our analysis indicates that no area is unaffected by human influence and that a large fraction (41%) is strongly affected by multiple drivers. However, large areas of relatively little human impact remain, particularly near the poles. The analytical process and resulting maps provide flexible tools for regional and global efforts to allocate conservation resources; to implement ecosystem-based management; and to inform marine spatial planning, education, and basic research.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Halpern, Benjamin S -- Walbridge, Shaun -- Selkoe, Kimberly A -- Kappel, Carrie V -- Micheli, Fiorenza -- D'Agrosa, Caterina -- Bruno, John F -- Casey, Kenneth S -- Ebert, Colin -- Fox, Helen E -- Fujita, Rod -- Heinemann, Dennis -- Lenihan, Hunter S -- Madin, Elizabeth M P -- Perry, Matthew T -- Selig, Elizabeth R -- Spalding, Mark -- Steneck, Robert -- Watson, Reg -- New York, N.Y. -- Science. 2008 Feb 15;319(5865):948-52. doi: 10.1126/science.1149345.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Center for Ecological Analysis and Synthesis, 735 State Street, Santa Barbara, CA 93101, USA. halpern@nceas.ucsb.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18276889" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Climate ; Conservation of Natural Resources ; *Ecosystem ; Fisheries ; *Human Activities ; Humans ; Mathematics ; Models, Theoretical ; Oceans and Seas
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2008-10-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stone, Richard -- New York, N.Y. -- Science. 2008 Oct 10;322(5899):184. doi: 10.1126/science.322.5899.184.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18845724" target="_blank"〉PubMed〈/a〉
    Keywords: *Angiosperms ; Animals ; Biodiversity ; *Bivalvia ; China ; Cyprinidae ; *Ecosystem ; Eutrophication ; Fisheries ; *Fishes ; *Fresh Water ; Population Dynamics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
    Publication Date: 2008-10-04
    Description: Atlantic bluefin tuna populations are in steep decline, and an improved understanding of connectivity between individuals from eastern (Mediterranean Sea) and western (Gulf of Mexico) spawning areas is needed to manage remaining fisheries. Chemical signatures in the otoliths of yearlings from regional nurseries were distinct and served as natural tags to assess natal homing and mixing. Adults showed high rates of natal homing to both eastern and western spawning areas. Trans-Atlantic movement (east to west) was significant and size-dependent, with individuals of Mediterranean origin mixing with the western population in the U.S. Atlantic. The largest (oldest) bluefin tuna collected near the northern extent of their range in North American waters were almost exclusively of western origin, indicating that this region represents critical habitat for the western population.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rooker, Jay R -- Secor, David H -- De Metrio, Gregorio -- Schloesser, Ryan -- Block, Barbara A -- Neilson, John D -- New York, N.Y. -- Science. 2008 Oct 31;322(5902):742-4. doi: 10.1126/science.1161473. Epub 2008 Oct 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Marine Biology, Texas A&M University, 5007 Avenue U, Galveston, TX 77551, USA. rookerj@tamug.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18832611" target="_blank"〉PubMed〈/a〉
    Keywords: *Animal Migration ; Animals ; Atlantic Ocean ; Carbon Isotopes/analysis ; Ecosystem ; Fisheries ; *Homing Behavior ; Likelihood Functions ; Mediterranean Sea ; Otolithic Membrane/chemistry ; Oxygen Isotopes/analysis ; Population Density ; Population Dynamics ; Reproduction ; Tuna/growth & development/*physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 11
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2009-08-22
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rick, Torben C -- Erlandson, Jon M -- New York, N.Y. -- Science. 2009 Aug 21;325(5943):952-3. doi: 10.1126/science.1178539.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Archaeobiology Program, Department of Anthropology, National Museum of Natural History, Smithsonian Institution, Washington, DC 20013, USA. rickt@si.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19696338" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Anthropology ; Archaeology ; *Ecosystem ; *Environment ; Fisheries ; Fishes ; Humans ; Marine Biology ; Otters ; Population Dynamics ; Sea Urchins ; Shellfish
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 12
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2009-05-30
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Morell, Virginia -- New York, N.Y. -- Science. 2009 May 29;324(5931):1132. doi: 10.1126/science.324_1132.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19478158" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Fisheries ; *Humpback Whale ; Oceans and Seas ; Population Dynamics ; Ussr
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 13
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1997-10-27
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Potera, C -- New York, N.Y. -- Science. 1997 Oct 10;278(5336):225-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9340769" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Eukaryota/*growth & development/isolation & purification ; Fish Diseases/diagnosis/*parasitology ; Fisheries ; Montana ; Oligochaeta/parasitology ; Oncorhynchus mykiss/*parasitology ; Polymerase Chain Reaction ; Protozoan Infections/diagnosis/parasitology ; *Protozoan Infections, Animal ; RNA, Protozoan/genetics ; RNA, Ribosomal/genetics ; Spores/physiology ; Temperature
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 14
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-05-14
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Inman, Mason -- New York, N.Y. -- Science. 2005 May 13;308(5724):937.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15890852" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Climate ; *Ecosystem ; Environment ; Fisheries ; *Fishes ; North Sea ; Population Dynamics ; *Seawater ; Temperature
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 15
    Publication Date: 2005-07-26
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kraus, Scott D -- Brown, Moira W -- Caswell, Hal -- Clark, Christopher W -- Fujiwara, Masami -- Hamilton, Philip K -- Kenney, Robert D -- Knowlton, Amy R -- Landry, Scott -- Mayo, Charles A -- McLellan, William A -- Moore, Michael J -- Nowacek, Douglas P -- Pabst, D Ann -- Read, Andrew J -- Rolland, Rosalind M -- New York, N.Y. -- Science. 2005 Jul 22;309(5734):561-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Edgerton Research Laboratory, New England Aquarium, Boston, MA 02110-3399, USA. skraus@neaq.org〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16040692" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Atlantic Ocean ; Ecology ; *Ecosystem ; Environment ; Female ; Fisheries ; Male ; Mortality ; Population Dynamics ; Population Growth ; Public Policy ; Reproduction ; Ships ; *Whales/physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 16
    Publication Date: 2005-07-30
    Description: The open oceans comprise most of the biosphere, yet patterns and trends of species diversity there are enigmatic. Here, we derive worldwide patterns of tuna and billfish diversity over the past 50 years, revealing distinct subtropical "hotspots" that appeared to hold generally for other predators and zooplankton. Diversity was positively correlated with thermal fronts and dissolved oxygen and a nonlinear function of temperature (approximately 25 degrees C optimum). Diversity declined between 10 and 50% in all oceans, a trend that coincided with increased fishing pressure, superimposed on strong El Nino-Southern Oscillation-driven variability across the Pacific. We conclude that predator diversity shows a predictable yet eroding pattern signaling ecosystem-wide changes linked to climate and fishing.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Worm, Boris -- Sandow, Marcel -- Oschlies, Andreas -- Lotze, Heike K -- Myers, Ransom A -- New York, N.Y. -- Science. 2005 Aug 26;309(5739):1365-9. Epub 2005 Jul 28.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Biology Department, Dalhousie University, Halifax, NS, Canada B3H 4J1. bworm@dal.ca〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16051749" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Biodiversity ; Climate ; *Ecosystem ; Fisheries ; Oceans and Seas ; Oxygen/analysis ; *Perciformes ; Population Density ; *Predatory Behavior ; Regression Analysis ; Seasons ; Temperature ; Time Factors ; *Tuna ; Zooplankton
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 17
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-05-10
    Description: Here we describe the requirements of an e-Infrastructure to enable faster, better, and different scientific research capabilities. We use two application exemplars taken from the United Kingdom's e-Science Programme to illustrate these requirements and make the case for a service-oriented infrastructure. We provide a brief overview of the UK "plug-and-play composable services" vision and the role of semantics in such an e-Infrastructure.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hey, Tony -- Trefethen, Anne E -- New York, N.Y. -- Science. 2005 May 6;308(5723):817-21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Engineering and Physical Sciences Research Council, Polaris House, North Star Avenue, Swindon SN2 1ET, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15879209" target="_blank"〉PubMed〈/a〉
    Keywords: Chemical Phenomena ; Chemistry ; Combinatorial Chemistry Techniques ; *Computational Biology ; *Computer Communication Networks ; *Computing Methodologies ; Databases as Topic ; Graves Disease/genetics ; *Internet ; *Research ; *Software ; Williams Syndrome/genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 18
    Publication Date: 2005-11-19
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bhattacharjee, Yudhijit -- New York, N.Y. -- Science. 2005 Nov 18;310(5751):1110-1.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16293737" target="_blank"〉PubMed〈/a〉
    Keywords: Animal Migration ; Animals ; Conservation of Natural Resources/*history ; Fisheries ; *Gadus morhua/physiology ; History, 20th Century ; History, 21st Century ; Maine ; Public Policy ; Reproduction
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 19
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-12-13
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sayles, Jesse S -- Green, David M -- New York, N.Y. -- Science. 2005 Dec 9;310(5754):1616-8; author reply 1616-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16339430" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Atlantic Ocean ; Canada ; *Ecosystem ; Environment ; Fisheries ; *International Cooperation ; Mortality ; Population Dynamics ; Public Policy ; Ships ; United States ; *Whales
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 20
    Publication Date: 2005-06-11
    Description: Removal of top predators from ecosystems can result in cascading effects through the trophic levels below, completely restructuring the food web. Cascades have been observed in small-scale or simple food webs, but not in large, complex, open-ocean ecosystems. Using data spanning many decades from a once cod-dominated northwest Atlantic ecosystem, we demonstrate a trophic cascade in a large marine ecosystem. Several cod stocks in other geographic areas have also collapsed without recovery, suggesting the existence of trophic cascades in these systems.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Frank, Kenneth T -- Petrie, Brian -- Choi, Jae S -- Leggett, William C -- New York, N.Y. -- Science. 2005 Jun 10;308(5728):1621-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Fisheries and Oceans, Bedford Institute of Oceanography, Ocean Sciences Division, Post Office Box 1006, Dartmouth, Nova Scotia, B2Y 4A2, Canada. frankk@mar.dfo-mpo.gc.ca〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15947186" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Atlantic Ocean ; Biodiversity ; Biomass ; *Ecosystem ; Fisheries ; *Fishes ; *Food Chain ; Gadus morhua ; *Invertebrates ; *Phytoplankton ; Population Density ; Population Dynamics ; Predatory Behavior ; Principal Component Analysis ; Seals, Earless ; *Seawater ; Time Factors ; *Zooplankton
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 21
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-10-22
    Description: If you trust the conventional wisdom, Amy Palmer and Alexis Templeton did a lot of things wrong in their job search. Then why did things turn out so right?〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Austin, Jim -- New York, N.Y. -- Science. 2005 Oct 21;310(5747):518-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16239480" target="_blank"〉PubMed〈/a〉
    Keywords: *Career Choice ; *Career Mobility ; Chemical Phenomena ; Chemistry ; Education, Graduate ; *Faculty ; Microbiology ; *Research ; United States ; *Universities
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    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 22
    Publication Date: 2005-04-23
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Avasthi, Amitabh -- New York, N.Y. -- Science. 2005 Apr 22;308(5721):487-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15845821" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Biodiversity ; California ; *Conservation of Natural Resources ; *Ecosystem ; Environment ; Fisheries ; *Fishes ; Marine Biology ; *Seawater
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 23
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-01-08
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Xian, Weiwei -- Kang, Bin -- Liu, Ruiyu -- New York, N.Y. -- Science. 2005 Jan 7;307(5706):41.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15637253" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; China ; *Ecosystem ; Fisheries ; Fishes ; *Rivers ; Scyphozoa/*growth & development ; Seasons ; Seawater
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-04-02
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stokstad, Erik -- New York, N.Y. -- Science. 2005 Apr 1;308(5718):41-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15802579" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biodiversity ; Conservation of Natural Resources ; Costs and Cost Analysis ; Developed Countries ; Developing Countries ; *Ecology/methods ; *Ecosystem ; *Environment ; Eutrophication ; Fisheries ; Humans ; International Cooperation ; Poverty ; Public Policy ; Trees
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-09-17
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stone, Richard -- New York, N.Y. -- Science. 2005 Sep 16;309(5742):1805-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16166491" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biomass ; *Ctenophora/growth & development/physiology ; *Ecosystem ; Feeding Behavior ; Fisheries ; Fishes ; Food Chain ; International Cooperation ; Iran ; Oceans and Seas ; *Pest Control, Biological ; Population Dynamics ; *Zooplankton
    Print ISSN: 0036-8075
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-10-08
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pala, Christopher -- New York, N.Y. -- Science. 2005 Oct 7;310(5745):37.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16210510" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Commerce/*legislation & jurisprudence ; Conservation of Natural Resources/*legislation & jurisprudence ; *Eggs ; Fisheries ; *Fishes ; Internationality ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-05-14
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Boersma, P Dee -- Vargas, Hernan -- Merlen, Godfrey -- New York, N.Y. -- Science. 2005 May 13;308(5724):925.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15890846" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Biodiversity ; Biological Evolution ; *Conservation of Natural Resources/legislation & jurisprudence ; *Ecosystem ; Ecuador ; Emigration and Immigration/legislation & jurisprudence ; Fisheries ; Humans ; *Population Growth ; Travel
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  • 28
    Publication Date: 2005-05-14
    Description: We show that the distributions of both exploited and nonexploited North Sea fishes have responded markedly to recent increases in sea temperature, with nearly two-thirds of species shifting in mean latitude or depth or both over 25 years. For species with northerly or southerly range margins in the North Sea, half have shown boundary shifts with warming, and all but one shifted northward. Species with shifting distributions have faster life cycles and smaller body sizes than nonshifting species. Further temperature rises are likely to have profound impacts on commercial fisheries through continued shifts in distribution and alterations in community interactions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Perry, Allison L -- Low, Paula J -- Ellis, Jim R -- Reynolds, John D -- New York, N.Y. -- Science. 2005 Jun 24;308(5730):1912-5. Epub 2005 May 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centre for Ecology, Evolution and Conservation, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK. a.perry@uea.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15890845" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Body Size ; *Climate ; *Ecosystem ; Environment ; Fisheries ; *Fishes/anatomy & histology/growth & development ; North Sea ; Population Density ; Population Dynamics ; *Seawater ; Temperature ; Time Factors
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2005-06-18
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Grigg, Richard W -- Dollar, Steven J -- New York, N.Y. -- Science. 2005 Jun 17;308(5729):1740-2; author reply 1740-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15961652" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Anthozoa ; *Conservation of Natural Resources ; *Ecosystem ; Eukaryota ; Fisheries ; Hawaii ; Population Dynamics ; Seals, Earless ; Turtles ; Water Pollution
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  • 30
    Publication Date: 2006-01-10
    Description: Since the mass mortality of the urchin Diadema antillarum in 1983, parrotfishes have become the dominant grazer on Caribbean reefs. The grazing capacity of these fishes could be impaired if marine reserves achieve their long-term goal of restoring large consumers, several of which prey on parrotfishes. Here we compare the negative impacts of enhanced predation with the positive impacts of reduced fishing mortality on parrotfishes inside reserves. Because large-bodied parrotfishes escape the risk of predation from a large piscivore (the Nassau grouper), the predation effect reduced grazing by only 4 to 8%. This impact was overwhelmed by the increase in density of large parrotfishes, resulting in a net doubling of grazing. Increased grazing caused a fourfold reduction in the cover of macroalgae, which, because they are the principal competitors of corals, highlights the potential importance of reserves for coral reef resilience.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mumby, Peter J -- Dahlgren, Craig P -- Harborne, Alastair R -- Kappel, Carrie V -- Micheli, Fiorenza -- Brumbaugh, Daniel R -- Holmes, Katherine E -- Mendes, Judith M -- Broad, Kenneth -- Sanchirico, James N -- Buch, Kevin -- Box, Steve -- Stoffle, Richard W -- Gill, Andrew B -- New York, N.Y. -- Science. 2006 Jan 6;311(5757):98-101.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Marine Spatial Ecology Lab, School of BioSciences, University of Exeter, Prince of Wales Road, Exeter EX4 4PS, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16400152" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Anthozoa/growth & development ; Bahamas ; Biomass ; Body Size ; *Conservation of Natural Resources ; *Ecosystem ; Fisheries ; *Fishes ; *Perciformes/anatomy & histology ; Population Density ; Population Dynamics ; Predatory Behavior
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2006-12-23
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Guo, Jerry -- New York, N.Y. -- Science. 2006 Dec 22;314(5807):1860.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17185573" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; China ; *Conservation of Natural Resources ; *Dolphins ; Environment ; Extinction, Biological ; Fisheries ; *Fresh Water ; Population Density ; *Porpoises ; *Rivers ; Water Pollution
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2006-04-29
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enserink, Martin -- New York, N.Y. -- Science. 2006 Apr 28;312(5773):512.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16645058" target="_blank"〉PubMed〈/a〉
    Keywords: Chemistry ; China ; *Fatty Acids ; France ; History, 21st Century ; *Theft
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  • 33
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2006-11-11
    Description: Sea urchins are major components of marine communities. Their grazing limits algal biomass, and they are preyed upon by many predators. Purple sea urchins (Strongylocentrotus purpuratus) are among the best studied species. They live in environments that alternate between two stable states: luxuriant, species-rich kelp forests and sea urchin-dominated "barrens." The transition from one state to the other can be initiated by several factors, including the abundance of algal food, predators, storm intensities, and incidence of disease. Purple sea urchins compete with other grazers, some of which are important fishery resources (such as abalones and red sea urchins), and they are harvested for scientific research. Revelations from their genome will lead to a better understanding of how they maintain their ecological importance, and may in turn enhance their economic potential.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pearse, John S -- New York, N.Y. -- Science. 2006 Nov 10;314(5801):940-1.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Long Marine Laboratory, University of California, Santa Cruz, 100 Shaffer Road, Santa Cruz, CA 95060, USA. pearse@biology.ucsc.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17095690" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biodiversity ; *Ecosystem ; Feeding Behavior ; Fisheries ; Fishes ; Gastropoda ; Genome ; Kelp ; Population Dynamics ; Strongylocentrotus ; Strongylocentrotus purpuratus/genetics/*physiology
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  • 34
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2007-04-14
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McCauley, Douglas J -- New York, N.Y. -- Science. 2007 Apr 13;316(5822):200-1; author reply 200-1.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17431154" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biomass ; Conservation of Natural Resources ; *Ecosystem ; Fisheries ; Pacific Ocean ; Population Dynamics ; Predatory Behavior ; *Sharks ; *Tuna
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  • 35
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2007-02-10
    Description: Achieving a fundamental understanding of the phenomena that will underpin both global stewardship and future technologies in energy calls for a thoughtful balance between large-scale immediate solutions using existing technology and the fundamental research needed to provide better solutions in the 50-year period.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Whitesides, George M -- Crabtree, George W -- New York, N.Y. -- Science. 2007 Feb 9;315(5813):796-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA. gwhitesides@gmwgroup.harvard.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17289985" target="_blank"〉PubMed〈/a〉
    Keywords: Biomass ; Biotechnology ; Carbon Dioxide/chemistry ; Catalysis ; Chemical Phenomena ; Chemistry ; Electricity ; Electrodes ; *Energy-Generating Resources ; Environment ; Photosynthesis ; *Research ; Solar Energy
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  • 36
    Publication Date: 2007-06-30
    Description: Like all species, humans have exercised their impulse to perpetuate and propagate themselves. In doing so, we have domesticated landscapes and ecosystems in ways that enhance our food supplies, reduce exposure to predators and natural dangers, and promote commerce. On average, the net benefits to humankind of domesticated nature have been positive. We have, of course, made mistakes, causing unforeseen changes in ecosystem attributes, while leaving few, if any, truly wild places on Earth. Going into the future, scientists can help humanity to domesticate nature more wisely by quantifying the tradeoffs among ecosystem services, such as how increasing the provision of one service may decrease ecosystem resilience and the provision of other services.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kareiva, Peter -- Watts, Sean -- McDonald, Robert -- Boucher, Tim -- New York, N.Y. -- Science. 2007 Jun 29;316(5833):1866-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Nature Conservancy, 4245 North Fairfax Drive, Suite 100, Arlington, VA 22203, USA. pkareiva@tnc.org〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17600209" target="_blank"〉PubMed〈/a〉
    Keywords: *Agriculture ; Animal Husbandry ; Animals ; Animals, Domestic ; Cities ; Commerce ; *Conservation of Natural Resources ; Crops, Agricultural ; Disasters ; *Ecosystem ; Fisheries ; *Human Activities ; Humans ; Trees
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  • 37
    Publication Date: 2007-03-31
    Description: Impacts of chronic overfishing are evident in population depletions worldwide, yet indirect ecosystem effects induced by predator removal from oceanic food webs remain unpredictable. As abundances of all 11 great sharks that consume other elasmobranchs (rays, skates, and small sharks) fell over the past 35 years, 12 of 14 of these prey species increased in coastal northwest Atlantic ecosystems. Effects of this community restructuring have cascaded downward from the cownose ray, whose enhanced predation on its bay scallop prey was sufficient to terminate a century-long scallop fishery. Analogous top-down effects may be a predictable consequence of eliminating entire functional groups of predators.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Myers, Ransom A -- Baum, Julia K -- Shepherd, Travis D -- Powers, Sean P -- Peterson, Charles H -- New York, N.Y. -- Science. 2007 Mar 30;315(5820):1846-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, Dalhousie University, 1355 Oxford Street, Halifax, NS B3H 4J1, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17395829" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Atlantic Ocean ; Bivalvia ; Conservation of Natural Resources ; *Ecosystem ; *Elasmobranchii ; Fisheries ; *Food Chain ; Ostreidae ; Population Dynamics ; Population Growth ; Predatory Behavior ; *Sharks ; Skates (Fish)
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  • 38
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2007-12-22
    Description: The runners-up for 2007's Breakthrough of the Year include advances in cellular and structural biology, astrophysics, physics, immunology, synthetic chemistry, neuroscience, and computer science.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉New York, N.Y. -- Science. 2007 Dec 21;318(5858):1844-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18096772" target="_blank"〉PubMed〈/a〉
    Keywords: Algorithms ; Animals ; Cellular Reprogramming ; Chemical Phenomena ; Chemistry ; Cosmic Radiation ; Humans ; Imagination ; Memory ; Physical Phenomena ; Physics ; Pluripotent Stem Cells ; Receptors, Adrenergic, beta-2/chemistry ; *Science ; T-Lymphocyte Subsets/cytology/immunology
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  • 39
    Publication Date: 2007-10-06
    Description: Captive breeding is used to supplement populations of many species that are declining in the wild. The suitability of and long-term species survival from such programs remain largely untested, however. We measured lifetime reproductive success of the first two generations of steelhead trout that were reared in captivity and bred in the wild after they were released. By reconstructing a three-generation pedigree with microsatellite markers, we show that genetic effects of domestication reduce subsequent reproductive capabilities by approximately 40% per captive-reared generation when fish are moved to natural environments. These results suggest that even a few generations of domestication may have negative effects on natural reproduction in the wild and that the repeated use of captive-reared parents to supplement wild populations should be carefully reconsidered.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Araki, Hitoshi -- Cooper, Becky -- Blouin, Michael S -- New York, N.Y. -- Science. 2007 Oct 5;318(5847):100-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Zoology, 3029 Cordley Hall, Oregon State University Corvallis, OR 97331, USA. arakih@science.oregonstate.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17916734" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Animals, Domestic/genetics/physiology ; Animals, Wild/genetics/physiology ; *Breeding ; Female ; Fisheries ; Male ; Oncorhynchus mykiss/genetics/*physiology ; Oregon ; Population Dynamics ; *Reproduction ; Time Factors
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  • 40
    Publication Date: 2008-12-20
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Whitfield, John -- New York, N.Y. -- Science. 2008 Dec 19;322(5909):1786-7. doi: 10.1126/science.322.5909.1786.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19095921" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Birds ; Climate ; Ecosystem ; Fisheries ; *Fishes ; *Food Chain ; Humans ; North Sea ; Nutritive Value ; Oceans and Seas ; Pacific Ocean ; Population Dynamics ; Predatory Behavior ; *Sea Lions ; Seawater ; Temperature
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  • 41
    Publication Date: 2009-09-12
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Walker, Brian -- Barrett, Scott -- Polasky, Stephen -- Galaz, Victor -- Folke, Carl -- Engstrom, Gustav -- Ackerman, Frank -- Arrow, Ken -- Carpenter, Stephen -- Chopra, Kanchan -- Daily, Gretchen -- Ehrlich, Paul -- Hughes, Terry -- Kautsky, Nils -- Levin, Simon -- Maler, Karl-Goran -- Shogren, Jason -- Vincent, Jeff -- Xepapadeas, Tasos -- de Zeeuw, Aart -- New York, N.Y. -- Science. 2009 Sep 11;325(5946):1345-6. doi: 10.1126/science.1175325.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The Commonwealth Scientific and Industrial Research Organisation (CSIRO) Sustainable Ecosystems, Canberra, ACT 2601, Australia. brian.walker@csiro.au〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19745137" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Climatic Processes ; Communicable Diseases/drug therapy/epidemiology ; Drug Resistance ; Ecosystem ; *Environment ; Fisheries ; Health ; Humans ; *International Agencies ; *International Cooperation
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  • 42
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2009-11-07
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vogel, Gretchen -- New York, N.Y. -- Science. 2009 Nov 6;326(5954):788-91. doi: 10.1126/science.326_788.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19892956" target="_blank"〉PubMed〈/a〉
    Keywords: *Academies and Institutes/economics/organization & administration ; Anthropology ; Biology ; Chemistry ; Germany ; Germany, East ; Physics ; Research Personnel ; Universities
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  • 43
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2009-05-09
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Greene, Charles H -- Monger, Bruce C -- McGarry, Louise P -- New York, N.Y. -- Science. 2009 May 8;324(5928):733-4. doi: 10.1126/science.1173951.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Ocean Resources and Ecosystems Program, Department of Earth and Atmospheric Sciences, Cornell University, Ithaca, NY 14853, USA. chg2@cornell.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19423808" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Atlantic Ocean ; *Climate ; Cold Temperature ; *Ecosystem ; Female ; Fisheries ; Gadiformes/physiology ; Ovum/physiology ; Pandalidae/*physiology ; Phytoplankton/*physiology ; Population Dynamics ; Reproduction ; Salinity ; Seasons ; *Seawater/chemistry
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  • 44
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 6 (1995), S. 237-242 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Because of the recent trend in total hip arthroplasty to achieve direct contact of the endoprosthesis with cortical bone, comparison of bone apposition from cortical bone and cancellous bone was performed. The response of cortical and cancellous bone to hydroxylapatite was also compared. Implants in cortical bone demonstrated a higher interface shear strength and more bone apposition than those in cancellous bone over all time periods tested. Hydroxylapatite coating increased the interfacial shear and bone apposition in cortical bone at all time periods and in both the 3- and 6-week cancellous bone models. The potential effect of stress shielding (and subsequent bone resorption) by femoral stems ingrown with cortical bone is discussed. © 1995 John Wiley & Sons, Inc.
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  • 45
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    Journal of Applied Biomaterials 6 (1995), S. 249-258 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Ions released from implant surfaces are suspected of playing some role in osteolysis surrounding metal prostheses. To understand how ions may affect osteogenesis, previous work exposed osteogenic cells to metal ions to study acute cytotoxic responses. The purpose of this study was to assess the long-term effects of sublethal ion concentrations on osteogenic cell proliferation and function. Bone marrow stromal cells were harvested from juvenile rats and exposed to solutions of ions associated with Co-Cr-Mo and Ti-6Al-4V implants. Cells were cultured for up to 4 weeks and assayed for total protein, alkaline phosphatase, osteocalcin, and calcium. Other than V+5, none of the ions affected cell proliferation, indicating that the chosen concentrations were sublethal as desired. V+5 elicited delayed gross toxicity not previously observed during acute experiments. At the chosen concentrations, Co+2, Cr+6, Mo+6, and Co-Cr-Mo alloy elicited little effect on cell proliferation and moderate effects on matrix mineralization. Cultures exposed to Ti+4, Al+3, and Ti-6Al-4V alloy also showed no decrease in cell number, but did show near total suppression of osteocalcin secretion and matrix mineralization. These results suggest that ions released from Ti alloy implants may interfere with osteoblastic cell differentiation, contributing to periprosthetic osteolysis by impairing normal osteogenesis. © 1995 John Wiley & Sons, Inc.
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  • 46
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    Journal of Applied Biomaterials 6 (1995), S. 267-282 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Crestal bone loss is observed around various designs of dental implants. A possible cause of this bone loss is related to the stresses acting on periimplant bone. To investigate the relationship between stress state and bone loss, two-dimensional finite element models corresponding to bucco-lingual and mesio-distal sections of canine mandibles with one of two designs of porous-coated dental implants were analyzed. A fully porous-coated design consisting of a solid Ti6A14V core had a porous coating over the entire outer surface of the implant component, while a partially porous-coated design had the porous coating over the apical two-thirds of the implant surface only. Occlusal forces with axial and transverse components were assumed to act on the implant with interface bonding and effective force transfer at all porous coat-bone interfaces and no bonding for the non-porous-coated regions. The results of the analysis indicated that at most implant aspects (buccal, lingual, mesial, and distal), the equivalent stresses in crestal bone adjacent to the coronal-most, non-porous-coated zone of the partially porous-coated implants were lower than around the most coronal region of the fully porous-coated implants. The region of lower stresses around the partially porous-coated implants corresponded to observed areas of crestal bone loss in animal studies, suggesting that crestal bone loss in this case was due to bone disuse atrophy. A number of parameters of the finite element models were varied to determine the effect on the resulting stress fields and, therefore, possible long-term bone remodeling. Based on differences in observed bone structures by histological examination and results of finite element analyses with fully and partially porous-coated implants, an equivalent stress equal to 1.6 MPa was determined to be sufficient to avoid bone loss due to disuse atrophy in the canine mandibular premolar region. © 1995 John Wiley & Sons, Inc.
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  • 47
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  • 48
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    Keywords: Chemistry ; Polymer and Materials Science
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    Notes: A quantitative method of reporting surface degradation of the ultra-high molecular weight polyethylene (UHMWPE) tibial component from retrieved total knee replacements (TKR) was developed. Specific features include a qualitative assessment expressing the patterns in which the damage was detected as well as a quantitative summary of the observed degradation mechanisms. In addition, a method of measuring lower limb alignment changes with time is described and related to the observed damage patterns. Two case studies are presented. One case illustrated that changes in alignment resulted from factors other than wear. The damage observed on the tibial plateau appeared to occur subsequent to the changes in alignment. The second case illustrated that the wear of the UHMWPE tibial insert lead to the changes in the overall lower limb alignment. The methods described provide additional information regarding TKR failure mechanisms compared to reporting methods currently available. In particular, the collection of temporal alignment data at clinical follow-up visits enhanced the assessment of the retrieved TKR. © 1995 John Wiley & Sons, Inc.
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  • 49
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The purpose of this study was to determine whether small, phagocytosable particles of titanium alloy (Ti) and high-density polyethylene (HDPE) have an adverse effect on bone ingrowth. The bone harvest chamber (BHC) was implanted bilaterally in the proximal tibial metaphysis of six mature rabbits. The BHC has a transverse 1-mm wide pore providing a continuous canal through the chamber for tissue ingrowth. After an initial 6-week period for osseointegration of the BHC, the contents of the canal were harvested repeatedly at 3 weekly intervals. This could be done with the chamber in place, without disturbing its exterior surface or the surrounding bone. The carrier solution, 1% sodium hyaluronate (Healon) was implanted first. In subsequent implantations, Healon was mixed with particles of HDPE or Ti averaging 4.7 ± 2.1 and 3.0 ± 2.6 μm, respectively. The contralateral chamber was left empty and served as a control. The chambers were harvested repeatedly, alternating experimental and control sides. The sections from the control side, and those containing Healon alone demonstrated extensive trabecular bone in a fibrovascular stroma. The sections containing Ti alloy particles were qualitatively and quantitatively similar to the control sections and those containing Healon, except for the presence of small black granules of Ti alloy, dispersed in the fibrovascular stroma or phagocytosed by scattered macrophages. The sections containing HDPE particles were infiltrated and engulfed by mononuclear and multinuclear histiocytic cells in a highly fibrous stroma. The majority of the multinucleated cells present were interpreted as being foreign body giant cells. Less trabecular bone was seen in the HDPE group compared to the other groups. Using the parameters chosen for this experiment, it would appear that small, phagocytosable HDPE particles are more deleterious to net formation of bone compared to particles of Ti alloy. © 1995 John Wiley & Sons, Inc.
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  • 50
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    Journal of Applied Biomaterials 6 (1995), S. 55-64 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: This study deals with a three-point flexure test for the metal-ceramic bond involving geometrically simple specimens (alloy strips partly coated with ceramic) that can be fabricated with reasonable expenditure and sufficient reproducibility. The calculation of the stress distribution in such specimens with the aid of the finite-element method (FEM) is presented. The aim of this numerical analysis is: to investigate the stress distribution in a ceramometallic specimen with dimensions that, in a large number of experiments, have proven to lead to debonding at one end of the ceramic veneer instead of a crack in the middle of the veneer; and to assign a bond strength to the measured critical bending force that takes into account the influence of the Young's modulus of the alloy as well as a possible deviation of the thickness of the metal substrate from the standard value. Bond strength values of a variety of metal-ceramic combinations are demonstrated. These experimental results demonstrate the reproducibility of the test method as well as its sensitivity to diverse parameters. The presented method is proposed as an alternative to another flexure bond test nominated for international standard. In this test ceramometallic strips are bent over a rod to a 90° angle of the specimen ends, subsequently flattened, and the fracture surface visually inspected for adherence of the ceramic to the alloy substrate along the predominant part of the middle third of the specimen. It is clear that such a test can at most deliver qualitative results. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 87-87 
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    Keywords: Chemistry ; Polymer and Materials Science
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  • 52
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    Keywords: Chemistry ; Polymer and Materials Science
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  • 53
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    Journal of Applied Biomaterials 6 (1995), S. 105-108 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Four different brands of bone cement (Palacos R, Simplex P, Sulfix, CMW 1) were tested for exothermic changes during polymerization at atmospheric pressure and under partial vacuum of 0.2 bar. Palacos R was also mixed at four pressure levels (1.0, 0.2, 0.12, and 0.05 bar). The peak temperature in the bone cement was 46 to 124 °C, depending on the measuring point. There was no difference in peak temperature or duration of temperature increase above 50 °C during the curing of cement whether mixed at atmospheric pressure or under partial vacuum at different pressure levels. © 1995 John Wiley & Sons, Inc.
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  • 54
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    Journal of Applied Biomaterials 6 (1995), S. 99-104 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: A major problem in fracture treatment by external fixation is screw loosening, which often results in reduced stability and can lead to prolonged treatment. A load-carrying experiment was conducted to determine whether coating implants with bioactive hyroxyapatite (HA) increases screw stability. Twelve HA-coated ASIF screws with 3 different macroporosities were inserted in 12 sheep that had already been fitted with a 6-pin external fixator for the treatment of a tibial osteotomy. The same number of uncoated polished steel screws served as controls. Although initial stability was not different for HA-coated screws, average removal torque after a 9-week implantation period increased with increasing macroporosity of the HA coating (p 〈.002). Instability of some screws was accompanied by histologic findings of cartilagenous tissue and proliferation of periosteal callus. Near the threads in the tibial cortex and in the shaft area of the screw were seen large numbers of HA particles that had been sheared off during implantation as well as during screw removal because of high contract forces between the HA coating and bone. Particulate debris of HA particles as well as the release of small bone fragments during explantation is likely to be unavoidable since HA adherence to bone is greater than adherence to steel after several weeks of implantation. © 1995 John Wiley & Sons, Inc.
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  • 55
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    Journal of Applied Biomaterials 6 (1995), S. 125-128 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Porous polyurethane foams were prepared from Daro foam components with a range of mechanical properties to simulate human trabecular bone. Ratios of 10.0:5.0, 10.0:7.9, and 10.0:10.0 isocyanate to resin were mixed, cured, and cut into cubes. Properties were determined from uniaxial compression to 50% of the original cube height at a strain rate of 1.2 mm/s. Electron microscopy was used to characterize the foam structure. Average compressive yield stress values, ultimate compressive stresses, and elastic moduli ranged from 4.44 to 2.79, 5.61 to 3.28, and 134.0 to 110.1 MPa, respectively, for the three formulations. The foam materials showed a similar morphology of spherical bubbles, and the average bubble size tended to decrease as the ratio of isocyanate to resin increased even though the bubble size differences were not statistically significant. The results indicate that large blocks of foam can be prepared with consistent mechanical properties simulating a range of trabecular bone properties so that implants can be tested for various patient populations. © 1995 John Wiley & Sons, Inc.
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  • 56
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    Journal of Applied Biomaterials 6 (1995), S. 129-135 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Bioabsorbable films show promise in preventing postoperative interfacial tissue adhesion. Absorbable polymers in film form are generally more sensitive to chemical environments, due to their large surface area to volume ratio. The in vivo environment contains lipids such as cholesterol, triglycerides, and phospholipids, which are known to affect the degradation of permanent and absorbable polymeric biomateials. Preliminary investigations of in vitro lipid exposure of bioabsorbable poly(ortho ester) (POE) films for implant use are described. POE has been studied previously for use in controlled drug delivery and fracture fixation. Six-week in vitro exposure of 65:35 POE films to a cholesterol emulsion (1 g/L) showed no apparent difference in hydrolytic degradation rates of mechanical properties or mass loss compared to deionized water exposed films. Decreases of 28 and 6% in inherent viscosity were observed after 5 weeks for cholesterol and deionized water exposed films, respectively, suggesting cholesterol may have some effect. Further examination of the data, due to sample variation, revealed that clear, uniform films showed only minor changes in mass loss and mechanical properties after 6 weeks in either of the in vitro media. But slightly cloudy films possessing microscopic bubbles showed accelerated degradation in both media, indicating the effect of cholesterol was inconclusive due to sample variation. Control of the microbubble formation process could have utility in controlling hydrolytic degradation of POE films. © 1995 John Wiley & Sons, Inc.
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  • 57
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    Journal of Applied Biomaterials 6 (1995), S. 171-174 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Grit blasting is a common procedure of roughening surfaces to promote physical attachment of porous coatings, but it has been shown to reduce fatigue strength. Shot peening is known to increase fatigue strength by inducing compressive surface stresses; however, it is not known how subsequent grit blasting affects these benefits. This study examines the endurance limits, Se, of ELI grade Ti-6A1-4V specimens under rotating cyclic bending, including polished (control); belted and beaded; belted, beaded, and grit blasted; and belted, beaded, shot peened, and grit blasted. Belting and beading resulted in a slight increase in Se; grit blasting caused a 15% reduction in Se from polished. Fifty percent of this reduction was recovered when shot peening preceded grit blasting, suggesting that residual compressive surface stresses, induced by peening, were not eliminated by the blast process. Roughness averages and RMS values did not correlate with Se trends. SEM results showed classical fatigue fractures, consistent with surface crack initiation. © 1995 John Wiley & Sons, Inc.
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  • 58
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    Journal of Applied Biomaterials 6 (1995), S. 175-183 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Concentrated fibrinogen was prepared from whole blood by cryoprecipitation or chemical precipitation and combined with thrombin to make fibrin glue (FG). Surgical application of FG include control of bleeding, adhesion of tissues, and sealing of tissue defects. The purpose of this study was to compare cryoprecipitation (cryo) of fibrinogen to precipitation using ethanol, ammonium sulfate (AS), and poly(ethylene glycol) (PEG). Our results suggest that AS precipitation is as effective as cryo in yielding fibrin glues with high bond strengths and is more effective than ethanol and PEG precipitation. In addition, the volume of FG per milliliter of plasma is greater after AS precipitation than after a single freeze-thaw cycle. It is concluded that AS is an efficient means for preparing FG from autologous blood. © 1995 John Wiley & Sons, Inc.
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  • 59
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    Journal of Applied Biomaterials 6 (1995), S. 185-190 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Biological materials have been used as prosthetic devices such as heart valves, vascular grafts, and pericardial patches. These biological materials have to be fixed with crosslinking reagents and sterilized subsequently before they can be implanted in humans. Recently, a new crosslinking reagent, epoxy compound, has been used to fix bioprostheses. In this fixation technique, heparin may be ionically bound on the tissue surface. It has been shown that the amount of heparin bound to the tissue surface is proportional to the quantity of protamine impregnated in the biological tissues. However, it is not known if the impregnation of protamine will affect the crosslinking density of the biological tissues. This study was designed to compare the crosslinking densities of the epoxy compound fixed biological tissues with or without heparinization. Fresh porcine aortic valves procured from a slaughter house were first impregnated in various concentrations of protamine sulfate (0, 0.5, 1.0, or 1.5%) for about 30 min. The porcine aortic valves were then crosslinked in a 4% epoxy compound solution (Denacol® EX-313). The porcine samples were taken out at various elapsed fixation periods: 18, 25, 48, 72, 96, and 120 h. Finally, the crosslinked porcine aortic valves were heparinized in a 0.5% sodium heparin solution for about 1 h. The crosslinking densities of the porcine leaflet and the aortic wall of each sample were determined by measuring their shrinkage temperatures. It was revealed that the impregnation of various concentrations of protamine did not seem to significantly alter the shrinkage temperatures of the porcine leaflet and the aortic wall throughout the entire fixation process (p 〉 0.05). This indicated that the impregnation of protamine did not significantly change the crosslinking density of the biological tissues. However, it was found that protamine tended to discolor the tissue and to stiffen the porcine leaflet and the aortic wall. Although the impregnation of protamine did not seem to significantly alter the crosslinking density of the epoxy compound fixed biological tissues, the increase of the stiffness of the heparinized tissues may cause concerns in some clinical applications. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 209-211 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Reducing the porosity of bone cement substantially strengthens the cement in fatigue. However, concern has been raised that reducing the porosity of the cement would increase the shrinkage of the cement and thus compromise the cement-bone interface. We measured diametral shrinkage of cement mantles prepared with and without centrifugation of the cement. Four cement preparations (centrifuged and uncentrifuged mixed with monomer at either room temperature or 0°C) were used to form cement mantles of four different thicknesses (2, 4, 6, and 8 mm) around a stainless steel rod. We conclude that porosity reduction dramatically increases the fatigue strength of bone cement without substantially changing the diametral dimensions of the polymerized cement when studied in the shape and volume of a cement mantle that would be used for total hip replacement. © 1995 John Wiley & Sons, Inc.
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  • 61
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    Journal of Applied Biomaterials 6 (1995), S. 225-230 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The distal migration of polyethylene wear debris appears to be a major cause of loosening and osteolysis of cementless total hip arthroplasties. The use of modern cementing techniques, circumferential porous or hydroxyapatite (HA) coating has been advocated as a means of preventing access of the particles to the bone-implant interface. The purpose of this study was to compare the ability of different methods of implant fixation to prevent wear debris migration. Three each of smooth, porous-coated, HA-coated, and cemented hemiarthroplasties were implanted in the right hip of 12 dogs. Polyethylene particles were injected into the hip once a month for 4 months and then the femurs were sectioned. The percentage bone ingrowth was 8% for the smooth, 38% for the porous, and 83% for the HA-coated sections. Infiltration of the interface membrane by histiocytes containing polyethylene particles, and endosteal scalloping or osteolysis were found on all sections of the smooth prostheses. No particles or osteolysis were found on any sections of the other three types of prostheses. Capsular and lymph node sections from all dogs revealed histiocytic infiltration with numerous polyethylene particles present. It is concluded that the use of cement, porous, or HA coating prevents or delays access of the polyethylene wear debris to the bone-implant interface. No one of these methods of fixation proved more advantageous in the 5-month limit of this study. © 1995 John Wiley & Sons, Inc.
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  • 62
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    Keywords: Chemistry ; Polymer and Materials Science
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    Notes: Thrombosis remains a significant and potentially catastrophic complication of polyethylene terephthalate (Dacron) prosthetic vascular graft implantation. Numerous attempts have been made to create a novel surface that reduces the adverse effects of blood interaction with the material. The purpose of this study was to create reactive groups on Dacron without significantly altering the chemical and physical properties of the biomaterial. These groups would then serve as “anchor sites” for covalent attachment of the blood protein albumin to the surface, thus creating a more biocompatible surface. Denier reduction, an established textile chemistry procedure that creates carboxyl groups on the fiber surface via hydrolysis of the material, was performed at 100°C using sodium hydroxide concentrations of 0.5, 1.0, 2.5, and 5.0% (treated materials referred to as 0.5% hydrolyzed etc.). Tensile strength determination of hydrolyzed materials revealed no statistically significant difference in material strength between control, 0.5, and 1.0% hydrolyzed materials; the 2.5 and 5.0% hydrolyzed materials had significant strength loss as compared to the controls. Significant fiber weight loss occurred in the 1.0, 2.5, and 5.0% hydrolyzed Dacron segments. The 0.5% hydrolyzed material did not have any significant weight loss. Covalent linkage of 125I-albumin to these modified materials using the crosslinker 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide hydrochloride (EDC) resulted in the 0.5% hydrolyzed material having the greatest protein binding (330 ng/mg Dacron, 2.4-fold greater than control). Incubation of the 0.5% hydrolyzed material with EDC and various concentrations of 125I-albumin resulted in the 14.80 m̈M solution permitting the greatest binding per milligram Dacron (330 ng/mg Dacron). Scanning electron microscopy, performed blindly, revealed no change in the 0.5% hydrolyzed Dacron as compared to untreated Dacron. The 5.0% hydrolyzed Dacron, however, had noticeable structural damage on the outer periphery of the fiber surface. Observation of the untreated Dacron with nonspecifically bound albumin showed scattered areas of albumin adherent to the fiber surface whereas covalent linkage of albumin to the 0.5% hydrolyzed Dacron via EDC crosslinking showed numerous albumin moieties on each fiber. This study demonstrates that a clinically accepted biomaterial (Dacron) can be chemically modified, without significantly altering the physical and chemical characteristics of the biomaterial, in order to covalently bind albumin to the fiber surface. Thus, these results serve as foundation for creating potential novel biomaterials without significantly altering the properties of the original biomaterial. © 1995 John Wiley & Sons, Inc.
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  • 63
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    Journal of Applied Biomaterials 6 (1995), S. 19-26 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The use of multiple-component systems in orthopedic surgery gives the surgeon increased flexibility in choosing the optimal implant, but introduces the possibility of interfacial corrosion. Such corrosion could limit the longevity of prostheses due either to tissue reactions to corrosion products, or to device failure. The incidence and nature of corrosion of modular total hips was evaluated in a consecutive series of 79 retrieved implants from University Hospitals of Cleveland. Surfaces were examined with stereo- and scanning electron microscopy. Several laboratory studies were undertaken to examine mechanisms that might contribute to the initiation of corrosion. The first set of experiments investigated the effect of head neck extension; the second study looked at the effect of material combinations on fretting corrosion and crevice corrosion. Analysis of retrieved implants demonstrated that fretting corrosion played a major role in the initiation of interface corrosion, and that a correlation existed between corrosion and length of neck extensions. Laboratory studies showed that longer head neck extensions may be more susceptible to fretting corrosion because of an instability at the interface. Short-term mixedmetal corrosion studies demonstrated that the coupling of cobalt and titanium alloys did not render the interface more susceptible to corrosion. It is hypothesized that fretting corrosion contributes to the initiation of modular interface corrosion, and that the problem can be reduced by design changes that increase the stability of the interface. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 35-41 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: In ligament replacement cases where the artificial ligament has worn and partially or totally ruptured, the released wear particles have been associated with a chronic synovitis that inhibits integration of the ligament and contributes to the ultimate failure of the device. In this study, the biological response to ligament wear particles from nine different artificial ligaments was quantitatively evaluated in vivo. Wear particles, the majority of which were 〈7 μm, were generated in vitro. These particles were injected into rabbit knee joints, and the biological response as well as the systemic migration of the particles were evaluated histologically after 4 weeks. The extent of the inflammatory reaction to the ligament wear particles was found to be significantly (p 〈 0.05) influenced by the type of material implanted. No particles were found in the regional lymph nodes or in the spleen, liver, kidney, or lung. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 43-53 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Two experimental methods for restoring flexor tendon sheath integrity and preventing adhesions around traumatized flexor tendons utilizing artificial tendon sheaths made of either hydroxyapatite (HAp) or alumina were studied in a flexor tendon-trauma model and compared to a standard tendon sheath repair and a control. Eighty toes were divided equally into a control group, a sheath repair group, an HAp group, and an alumina group. Profundus tendons in zone II were divided and repaired after sublimis excision in all groups. In the sheath repair group, the flexor sheath was also repaired after suturing the tendon. In artificial sheath groups, sheaths made of HAp and alumina were placed over the repair sites to protect them from the surrounding tissues. In the control group, after repairing the tendon, the flexor sheath was excised and no artificial sheaths were used. Each toe was immobilized in a plaster cast for 3 weeks. After three weeks, the plaster cast was removed followed by the removal of the sheaths in the artificial sheath groups through a small incision in the skin in zone II. Active mobilization was encouraged in each group. Postoperative adhesions were examined at 3, 6, 9, and 12 week intervals by using light microscopic techniques. To further explore the effects of artificial sheaths on tendon healing, transmission electron microscopy was done for the HAp and alumina groups at 3, 6, and 12 week intervals. Results demonstrated decreased severity of postoperative adhesions in the HAp as well as in the alumina groups in comparison with the sheath repair and controls. A space resembling the fibro-osseous canal was formed around the tendon after removing the sheaths. This space remained patent until 12 weeks, 9 weeks after removing the sheaths, and a newly formed tendon sheath-like structure lined by synovial cells and with a peritenon-like structure over the tendon surface was observed. In the sheath repair and control groups, the severity of adhesions was decreased with the passage of time, to some extent due to unrestricted mobility. However, a newly formed tendon sheath or peritenon-like structure was not observed. Electron microscopic studies confirmed good healing at the suture in the HAp and alumina groups with no evidence of necrosis. These results are qualitative in nature as no statistical tests were performed. From these results we conclude that if the tendon is separated from the surrounding granulation tissue by a barrier with good biocompatibility, the tendon can heal with fewer adhesions. Artificial sheaths may be used to reduce adhesions in severe injuries of the hand where postoperative immobilization is necessary due to fractures, vascular, or nerve injuries. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 65-68 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
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    Notes: A natural poly(hydroxybutyrate-co-9% hydroxyvalerate) copolyester was processed into a three-dimensional porous foam structure by salt leaching/solvent casting with previously sieved sodium chloride salts. Laboratory-built P(HB-9% HV) foams and commercial collagen sponges were cut into small rectangular specimens, sterilized, and prewetted using ethanol, rinsed with Dulbecco's minimum essential medium + 10% serum culture media, and seeded with fibroblasts isolated from canine anterior cruciate ligaments. The fibroblast cultures into such porous substrates were performed from 0 to 35 days by incubation (5% CO2) at 37°C. It demonstrated that the P(HB-HV) sustained a cell proliferation rate similar to that observed in collagen sponges, up to at least 35 days, with a maximal cell density on the day 28 in culture. On the other hand, the P(HB-HV) materials kept their structural integrity during the culture period while the collagen foams contracted greatly. Further, the total protein production after 4 weeks in culture was found to be twice as high (190 ± 10%) in the P(HB-9% HV) foam than in the collagen foam. Porous P(HB-HV) materials appear to be adequate polymeric substrates for cell cultures. However, further evaluations are still required to confirm such preliminary results. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 69-74 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Neodymium-iron-boron magnets are being increasingly used in orthodontic and orthopedic appliances. The purpose of this study was to investigate the in vitro cytotoxicity of these new “super” magnets. A modified agar overlay technique was performed using both L929 mouse fibroblasts and adult human oral mucosal fibroblasts. The neodyium-iron-boron magnets were tested in the following forms: uncoated magnetized, uncoated demagnetized, parylene coated demagnetized, and parylene coated magnetized. Results revealed that uncoated magnetized, uncoated demagnetized, and parylene coated magnetized were cytotoxic to both L929 mouse fibroblasts and human oral mucosal fibroblasts. However the parylene coated demagnetized neodymium magnets were only cytotoxic to the human mucosal fibroblasts. This study supports the hypothesis that these are cytotoxic effects of possibly corrosion products and of magnetism. The specific toxic effects of parylene were not examined in this present study. Statistical analysis (Mann-Whitney U-test, p = 0.014) suggested that, in this experiment, the human oral mucosal fibroblasts were more sensitive to the effects of these rare earth magnets. With the evidence of a biological effect, the use of implanted magnets must at present be limited until the safety can be assured. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995) 
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  • 69
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    Journal of Applied Biomaterials 6 (1995), S. 75-83 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: A new bone cement was developed with the purpose of reducing the adverse biological effects during cementation of implants. This bone cement is characterized by lower exotherm, low release of monomer, low residual content of monomer, and retained physical properties. The essential innovation was substitution of half of the methylmethacrylate (MMA) in the monomer with long chain, high molecular weight, less volatile, and less soluble methacrylates (n-decylmethacrylate, isobornyl-methacrylate), as well as alteration of the accelerator system to a mix of dihydroxypropyl-p-toluidine and N,N-dimethyl-p-toluidine. The powder contains butyl-methacrylate-MMA copolymers. These measures lower the glass-transition temperature, and permit more complete mixing in an integrated package, mixing, and delivery system consisting of a vacuum packed, double chamber pouch. The porosity was reduced to about 2% and the largest voids measured 0.1 mm. The polymerization exotherm was reduced to 58 °C. The compressive strength was 82 MPa, the four-point bending strength 55 MPa, the flexural modulus 2.24 GPa, the tensile strength 32 MPa, and the shear strength 36 MPa. The fracture toughness was 0.89 MPa √cm. These mechanical properties together with the fatigue life were on level with manually mixed, conventional PMMA bone cements. © 1995 John Wiley & Sons, Inc.
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    Notes: A Synthetic composite bone graft material, composed of fibrillar collagen, hydroxyapatite, and tricalcium phosphate, was evaluated in canine femoral shaft defects and compared to ungrafted defects and defects grafted with autogenous bone. The results of mechanical testing of the grafted femora in torsion at 1 year postoperatively were previously reported. This report details the histological and microscopic features of the graft sites. The results of this study suggest that the healing of the graft site is not yet complete at 1 year and that healing may be more complete in defects that received the synthetic graft as compared to ungrafted sites and autogenous bone graft. The bone tissue appears to respond to the graft particles as if they were bone particles. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 109-116 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Notes: The effects of metal ions released from orthopedic implants on nearby bone cells remain largely unknown. The purpose of this study was to examine the acute toxicity of metal ions on osteogenic cells derived from bone marrow. Bone marrow stromalcells were cultured with metal ions found in commonly used orthopedic implants, that is, Ti-6Al-4V, Co-Cr-Mo, and 316L stainless steel. Solutions of individual ions and combinations representing the alloy composition were prepared from atomic absorption standards and added to the cultures to give concentrations ranging from 50 ppb to 5o ppm. After a 48-h period of exposure to ions, the bone marrow cultures were examined for effects of cytotoxicity by measuring total cell number, total cell protein, and mitochondrial activity. Cr6+ was grossly cytotoxic; Co2+, Mo6+, Fe3+, and Ni2+ were moderately cytotoxic; and Ti4+, Al3+, V5+, and Mn2+ were minimally toxic, as determined by the assays used. Ion solutions representing Co-Cr-Mo and 316L stainless steel were moderately toxic; solutions representing Ti-6Al-4V were toxic at only the highest concentrations used. The observed cytotoxicity was time-dependent, with irreversible toxic effects being initiated following as short as a 3- to 6-hour exposure. These results show that metal ions associated with Co-Cr-Mo and 316L stainless steel are toxic to osteogenic cells at concentrations approximating those measured in the fibrous membrane encapsulating orthopedic implants. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 117-123 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Topics: Medicine , Technology
    Notes: The main function of the intervertebral disc is to transmit and attenuate compressive and torsional forces, and stabilize the intervertebral joint. Unfortunately, the disc may be displaced or damaged due to trauma or disease causing the nucleus to herniate and protrude into the vertebral canal or intervertebral foramen. Pressure on the spinal nerve may cause pain or paralysis in the area of its distribution. At present, the surgical procedures used to alleviate this condition include disc excision, and/or spinal fusion. A more desirable situation would involve removing the nucleus pulposus and part or all of the annulus fibrosis and implanting a suitable biofunctional equivalent. Such a prostheis should attenuate stresses and prevent abnormal stress at adjacent intervertebral joints. Maintenance of normal disc height would prevent impingement of the posterior facet joints and facet joint syndrome. In a previous companion paper (J. Applied Biomat. 5:125-132; 1994), the mechanical behavior of disc prostheses manufactured from fiber reinforced, elastomeric thermoset resins were examined. In order to develop devices which were more practical from a manufacturing standpoint and extremely reproducible, the fiber reinforced thermoset resins were replaced by multi-durometer thermoplastic elastomeric materials. In the present paper, the mechanical properties of thermoplastic multicomponent desings have been investigated. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 137-144 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Topics: Medicine , Technology
    Notes: The research field of biomaterials and surgical implants has matured to a point suggesting that a formal and comprehensive education is now required to handle all professional issues related to biomaterials and implant development. A professional curriculum is proposed for a discipline of biomaterials science and engineering on a graduate level. The curriculum includes the definition of an essential knowledge base and describes two track options for a study period of 3 years. Lists of prerequisites as well as required and suggested courses are presented and discussed. Continuing education courses are presented as examples. A quick vision of the immediate future of the field enforces the need for biomaterials professionals to take the lead in bringing the field into the next century. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995) 
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    Journal of Applied Biomaterials 6 (1995), S. 167-169 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: A novel drug delivery system for osteomyelitis was developed using porous hydroxyaptite blocks (HA-b) loaded with antibodies by centrifugation. In the study, 10 mm3 HA-b was placed in a container and mixed with antibiotic solution; the antibiotic was then loaded into the pores of the HA-b by centrifuging at 1500 rpm for 15 min. Slow release of HA-b in both moist form and dried form (by heating at 160°C) was tested after loading with the antibiotic arbekacin sulfate (ABK), 1-N-(S)-4 amino-2-hydroxybutyryl dibekacin. To estimate the concentration of antibiotic, both forms of HA-b were placed in 3 mL of phosphate buffered saline (PBS), which was replaced every 48 h. In both groups, which were loaded with 70 mg ABK per one block of HA (concn 0.5 μg/mL) which is sufficiently high to control most pathogens, was maintained for 21 exchanges of PBS (after 42 days). Minimum inhibitory concentration for methicillin-resistant Staphylococcus aureus (MRSA), 3.13 μg/mL, was maintained until nine exchanges took place (after 18 days). The centrifugation method is wsimple, and driked ABK produced by heating loaded HA-b is particularly useful in clinical applications for osteomyelitis. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 191-197 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: In this study, reinforced poly(L-lactic acid) (PLLA) fibers made by a dry-spinning/hot-drawing process were evaluated for use as a suture. The initial tensile strength of the PLLA fibers was lower than the initial tensile strength of the commercially available sutures: PDS, VicrylTM, silk, and Ethilon™. However, after 12 weeks immersion in a phosphate saline buffer at 37°C, PDS sutures have lower tensile strength than PLLA sutures and the tensile strength of Vicryl™ was unmeasurable because of fragmentation. Initially, PLLA fibers distinegrated into fibrils during degradation triggering an inflammatory response comparable to degradable multifilament sutures. However, the intensity of the inflammatory response against the PLLA fibers decreased and after 80 weeks implantation in the muscle layer of the abdominal wall of rats it was comparable to the one against Ethilon™. The inflammatory response against Ethilon™, which is considered to be nondegradable, increased in the same period, probably due to the change in shape. In practice, the handling characteristics of PLLA sutures are superior to the monofilament sutures like PDS and EthilonTM and comparable with the multifilament sutures like VicrylTM and silk. The knot security, but this remains to be investigated. It is concluded that dry-spun/hot-drawn (reinforced) PLLA fibers have the potential for use as long-term degradable suture material. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 203-208 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Journal of Applied Biomaterials 6 (1995), S. 223-223 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Journal of Applied Biomaterials 6 (1995), S. 213-218 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Notes: The occlusion and stability of five synthetic plugs used to restrict the femoral canal prior to cemented arthroplasty was assessed. A model was employed consisting of a hollow wooden dowel to simulate the canal, with adapters fixed to both ends to accommodate cement insertion and pressurization, and to produce a closed distal cavity. Three different canal diameters within the range accommodated by the plug selected were employed to assess insertion force, distal pressure during both insertion and pressurization of acrylic cement, and plug migration and leakage for each device. There was a wide variation among plugs in the magnitudes of the force and distal pressure upon plug insertion. During cement pressurization, cement leakage and/or plug migration was noted in all trials, and either of these events resulted in pressure rises in the distal cavity. Most notably, differences of only 0.5 mm in the diameter of the canal resulted in marked changes in the occlusion and stability achieved. It is concluded that for the range of canal sizes anticipated intraoperatively, these synthetic plugs appear deficient to varying extents in their ability to occlude or remain stable in the intramedullary canal. © 1995 John Wiley & Sons, Inc.
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    Notes: The purpose of this study was to compare hydroxyapatite (HA)-coated titanium (Ti) and HA-coated cobalt-chrome (CoCr) implants in the distal femur of the rabbit by evaluating bone apposition and interfacial shear strength. Bilateral cylindrical implants with a plasma sprayed 50-m̈m thick HA coating were press-fit into the metaphyseal cancellous bone of the lateral femoral condyles in a transverse fashion, and the animals were sacrificed at 2, 4, and 8 weeks postimplantation. Mechanical strength of the interface between HA and bone was measured using the pushout method. For histologic analysis, the fractional linear extent of bone apposition was quantitated. No differences were found in the interfacial shear strength between the Ti and CoCr at any time period. The amount of bone apposition increased significantly at each time interval for both substrate metals, but there were no significant differences between the two substrates at any of the time periods studied. The HA-coated CoCr implants performed in a similar manner to the HA-coated Ti implants, both mechanically and histologically, suggesting that HA-coated CoCr implants deserve further study as a viable alternative to Ti for the biological fixation of total joint components in orthopaedic surgery. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 243-248 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Topics: Medicine , Technology
    Notes: Historically, pyrolytic carbon has been a material for cardiovascular applications, but it has several properties suited for orthopedic uses as well. Pyrolytic carbon has an elastic modulus similar to bone and is highly fatigue resistant, but has not been used in orthopedics because of poor fixation to bone. Plasma sprayed hydroxylapatite (HA) has significantly improved the bonding of bone to titanium alloy implants. The effect of plasma-sprayed HA on pyrolytic carbon implants was investigated in this study. Cylindrical samples were implanted through a single cortex in Beagle femurs. The animals were sacrificed after 8 weeks, and a mechanical push-out test was performed on the implants immediately after explantation. Samples were microradiographed, stained for histology, and examined histomorphometrically. Interface strength for each type of implant was calculated. Pyrolytic carbon showed almost no attachment strength with an average strength value of 1.59 MPa. HA-coated pyrolytic carbon (8.71 MPa) yielded the same interfacial strength as HA-coated titanium (8.71 MPa). Histology revealed that bone was in direct apposition to all implants, both HA coated and noncoated. Failures occurred between the core material and the coating, or within the coating, but not at the bone/HA interface. Histomorphometry results confirmed that the two types of HA-coated implants had more bone apposition than the uncoated pyrolytic carbon implants. It was concluded that a plasma sprayed HA coating significantly improves the bone fixation of pyrolytic carbon. © 1995 John Wiley & Sons, Inc.
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    Notes: Heparin oligosaccharides with different anticoagulant activities were prepared and immobilized onto pyrolytic carbon coated graphite (PC) heart valve materials commonly used in mechanical heart valve prostheses. Prior to immobilization, PC surfaces were modified by radio-frequency plasma polymerized N-vinyl-2-pyrrolidone (PPNVP) thin films (∼ 100 nm) and derivatized to provide surface hydroxyl groups. Cleaved, low affinity heparin (C-heparin) with factor Xa inhibition activity of 107 to 130 IU/mg, was prepared by partial deaminative cleavage of commercial crude heparin, and high-affinity heparin (HA-heparin) with factor Xa inhibition activity of 550 to 1000 IU/mg was prepared by fractionation of C-heparin using agarose-ATIII affinity chromatography. C-heparin and HA-heparin were immobilized to surface modified PC by reductive amination. Anticoagulant activity of the heparin immobilized surfaces was determined by chromogenic assay for the inhibition of factor Xa. Highest surface anticoagulant activity was measured on C-heparin immobilized surfaces (64.0 ± 7.3 mIU/cm2) compared with HA-heparin immobilized surfaces (27.2 ± 12.2 mIU/cm2), suggesting higher binding of C-heparin than HA-heparin on the modified PC surfaces. Immobilized surfaces were evaluated under dynamic flow conditions, by subjecting samples to shear stress of up to 206 dyn/cm2 in the presence of 5% albumin solution or human plasma. Anticoagulant activity of the immobilized heparin was retained, although reduced, and the modified surfaces showed evidence for protein resistance. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 283-288 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Notes: The effect of sodium fluoride on the polarization resistance of titanium was investigated. Titanium plates were exposed to sodium chloride solutions with increasing fluoride concentrations. This was done at pH 7 and 4 at 37°C. The polarization resistance technique was chosen because it is the only electrochemical corrosion test procedure that allows sequential measurements of the same specimen and provides a quantitative basis to estimate corrosion currents unlike measurements of the potential. The results showed a large decrease in polarization resistance with increasing fluoride concentration at pH 4. The polarization resistance at pH 7 remained constant after a slight decrease at a very high value, even with a high fluoride concentration. The results clearly confirm that titanium is attacked by fluoride in an acidic environment. The clinical implications are that fluoride rinses or fluoride gels must have a neutral pH if there is a titanium containing device in the oral environment despite the less prophylactic effectiveness. © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 303-304 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Journal of Applied Biomaterials 6 (1995), S. 1-7 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Surgical implants need to be free from contaminants before implantation. The effectiveness of a presently used Clemson bioengineering cleaning (CBC) protocol was evaluated for cleaning three different biomaterials (titanium, aluminum oxide, and polyethylene terephthalate, PET) contaminated with three different contaminants (calcium chloride, zinc chloride, and hexadecane). Radiolabeled tracer analysis (RTA), with the use of liquid scintillation, was used as the surface analytical technique to quantitatively determine the removed from the biomaterial surface. On average, the ultrasonic cleaning step removed 99.96% of all three contaminants from both titanium and aluminum oxide. The CBC protocol did not sufficiently clean PET fabric contaminated with hexadecane leaving 11.76% of the contaminant after the ultrasonic step. With the use of isopropyl alcohol in series with 1% Liquinox, the ultrasonic step cleaned the fabric soiled with hexadecane within 30 min, removing 99.85% of the hexadecane initially on the surface. RTA proved to be an excellent method of quantifying surface contamination on implant materials, and for assessing the effectiveness of cleaning protocols in question. © 1995 John Wiley & Sons, Inc.
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    Notes: In research animals, the immune adjuvant activity of silicone in eliciting antibodies to associated protein antigens is now well established. In humans, the immune adjuvant activity of silicone remains controversial. Clusters of data from various research groups are beginning to define the boundary conditions for future large epidemiological studies. In that spirit, this manuscript reports pilot clinical data from a serological study conducted 3 years ago and reported previously in this journal. Sera from a self-selected symptomatic population of patients with breast implants were assayed for elevated concentrations of antibodies showing binding avidity to silicone surface associated antigens [anti-SSAA(x)]. In that study of 249 patients, two distinct statistically significant subgroups were identified on the basis of the serological assay alone: patients without any elevated anti-SSAA(x) (negative) and patients with elevated anti-SSAA(x) (positive) (p 〈 0.001). In this study, a clinical survey returned by 226 of those patients was correlated with the previously acquired serological findings. The 11 most common clinical complaints reported by the 199 anti-SSAA(x) negative patients and by the 27 anti-SSAA(x) positive were compared and their frequencies analyzed. The 199 anti-SSAA(x) negative patients, as a group, showed a lower frequency of a variety of signs and symptoms compared to the 27 anti-SSAA(x) positive patients. Statistically significant differences were seen in three of the symptoms: fever, foot pain, and sleep disturbance (p 〈 0.05). In addition, the syndrome of fever in the absence of local chest pain was a significantly associated with anti-SSAA(x) positivity (p 〈 0.001). © 1995 John Wiley & Sons, Inc.
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    Journal of Applied Biomaterials 6 (1995), S. 161-165 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: We performed a histomorphological and morphometric analysis of the effects of short daily periods of micromotion and phagocytosable particles of high density polyethylene (PE) on bone ingrowth into a 1 × 1 × 5 mm canal within a titanium chamber in rabbits. The micromotion chamber (MC) was implanted in the tibia of nine mature New Zealand white rabbits. After osseointegration and first harvest of tissue, 40 micromotions (amplitude = 0.5 mm) were applied daily at a rate of 1 Hz for a 3-week period. The tissue within the chamber was then harvested. For the second treatment, PE particles (108/mL) were placed within the canal. The tissue in the chamber was harvested 3 weeks later. The next treatment was a 3-week rest period, in which neither micromotion nor particles were utilized; a harvest followed. The final treatment combined PE particles and micromotion, followed by a harvest 3 weeks later. Sections from control harvests contained extensive trabecular bone arranged longitudinally throughout the canal in a fibrovascular stroma. Micromotion produced longitudinally oriented fibrous tissue within the chamber. PE particles were associated with macrophages, surrounding and engulfing the birefringent particles. The combination of particles and micromotion produced a fibrous stroma laden with macrophages. PE particles and micromotion, alone or together, produced a similar effect in inhibiting bone ingrowth, compared to nonmoved chambers without particles. In this short-term experiment, no additive or potentiating effect of these two stimuli could be demonstrated. © 1995 John Wiley & Sons, Inc.
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  • 92
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    Journal of Applied Biomaterials 6 (1995), S. 199-202 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The biological effects of the corrosion products of neodymium iron boron (Nd2Fe14B) magnets are largely unknown. The aim of this study was to identify the types of corrosion product and to evaluate the effect of the corrosion products (CP) of Nd2Fe14B magnets on the proliferation of human oral mucosal fibroblasts. Uncoated Nd2Fe14B magnets were stored in saline at 37°C for 6 months and the corrosion products collected. 100 μL of a cell suspension (human oral mucosal fibroblasts [4 × 104 cell/mL]) was aliquoted into 72 wells of a 96-well plate, the remaining plates receiving culture medium only. After 12 h incubation at 37°C, each well then received 100 μL of either (A) culture medium, (B) 100% CP, (C) 50%, or (D) 0% CP. The plates were reincubated at 37°C for a further 48, 96 or 144 h. Fibroblast proliferation was assessed using the methylene blue uptake/elution technique. The compounds in the corrosion product were examined using quantitative X-ray analysis. Statistical analysis (ANOVA, Bonferroni's test 0.05, SAS v 6.04), showed that at each time point, the cell numbers in groups B, C. and D were significantly lower than group A. Within groups B, C, and D no significant differences were found, despite the suggestion of a dose response effect. Fibroblast proliferation in the presence of corrosion products was significantly lower than with culture medium. Fibroblast proliferation did occur in the presence of 0, 50, and 100% CP. The actual corrosion products appeared to be salts of iron but 3.2% (±0.6) of neodymium chloride (NDCl3) was found. © 1995 John Wiley & Sons, Inc.
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  • 93
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 6 (1995), S. 219-221 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
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  • 94
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 6 (1995) 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
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  • 95
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    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 29 (1995) 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
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  • 96
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    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 29 (1995), S. 19-24 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: We performed a transcortical push-out test to determine the effect of surface roughness of hydroxyapatite (HA)-coated implants on bone-implant shear strength in a canine model. Hydroxyapatite- and alumina-coated SUS316L with the same surface roughness (roughness average: Ra = 5 μm) and HA-coated Ti-6Al-4V (Ra = 8.4 μm), sintered HA (Ra = 0.9 μm), and dense alumina (Ra = 1.3 μm) were inserted into the dog's femur. The interface shear strength of the dense alumina was significantly lower than that of other implants at both 4 and 12 weeks after implantation. At 4 weeks after implantation, the interface shear strength of the alumina-coated SUS316L was significantly lower than that of other implants (P 〈 .05) except the dense alumina, but at 12 weeks, there was no significant difference between the implant types except the dense alumina. This indicates that the surface roughness of the HA coating affects the enhancement of the bone-implant interface shear strength at the early period after implantation, and that a surface roughness of several micrometers does not influence the bond strength between bone and HA. A scanning electron microscopic study indicated that in almost all cases at 12 weeks, the failure site after push-out testing was the coating-substrate interface, not the coating-bone interface. Therefore, protection of the coating-substrate interface from direct shear loading is needed. © 1995 John Wiley & Sons, Inc.
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  • 97
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    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 29 (1995), S. 47-57 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The morphology of bone may be influenced by many factors, including electromechanical ones such as electric potentials, electric fields, or zeta potentials. Stress-generated potential studies in bone and particle electrophoresis studies using calcium-deficient hydroxyapatite have shown that the zeta potential depends on the composition of the steeping fluid and steeping time. To better quantify and understand these in situ potential changes in bovine cortical bone, the effects of alterations in calcium, phosphate, and fluoride concentrations in Neuman's Fluid (NF), which simulates in vivo bone extracellular fluid, were investigated using particle electrophoresis. The zeta potential increased in magnitude with increased calcium concentration in NF in as little as 17 min. Increasing phosphate concentration in NF also increased the zeta potential magnitude. These results provide support for a structural model of the bone matrix surface-bone fluid interface, which incorporates the bone surface proper (composed of collagen, mineral, and boundary regions), stationary layer (in which ions, ionic complexes, and proteins may be adsorbed), and bone extracellular fluid. These results, coupled with those of previous studies, indicate that the protein phase probably has an important role in the determination of the physiologic zeta potential; the role of the mineral phase may also be important. © 1995 John Wiley & Sons, Inc.
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  • 98
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    Journal of Biomedical Materials Research 29 (1995), S. 89-99 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Dense, sintered, slip-cast hydroxyapatite rods were implanted transfemorally in young adult rats. The femora were excised after 2 and 4 weeks and, following fixation, either embedded in methyl methacrylate for light microscopy, decalcified and prepared for transmission electron microscopy, or freeze fractured in liquid nitrogen for scanning electron microscopic analysis. The latter was performed on the two tissue fragments that remained after freeze fracturing, from which the first contained the implants and the second comprised tissue that had been immediately adjacent to the hydroxyapatite rods. Undecalcified light microscopic sections revealed extensive bone tissue formation around and in contact with the hydroxyapatite rods. The initial bone matrix apposed to the implant surface, as demonstrated with scanning electron microscopy, was either composed of globular deposits or an organized network of collagen fibers. The deposits, which ranged in size from 0.1-1.1 μm, fused to form a cement-like matrix to which collagen fibers were attached. Degradation of the hydroxyapatite surface resulted in the presence of unidirectionally aligned crystallites, with which the newly formed bone matrix was closely associated. Ultrastructural analysis of the bone-hydroxyapatite interface with transmission electron microscopy revealed a 50-600-nm-wide collagen-free granular zone, comprising one or more 40-100-nm-thick electron-dense layer(s). These structural arrangements most probably partially represent the globular deposits and proteinaceous material adsorbed onto and partially in the degrading hydroxyapatite surface. Although the latter change in surface topography may have enhanced bonding of the cement-like matrix to the hydroxyapatite, the cause for this change in topography and the type of bond formed are, at present, unknown. © 1995 John Wiley & Sons, Inc.
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  • 99
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    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 29 (1995), S. 138-138 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
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  • 100
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    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 29 (1995), S. 309-314 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: This study assessed the effectivenness of finite element analysis in predicting the stress intensity factor (KIC) for three types of dental materials: a glass ionomer, a dental amalgam, and a composite resin. Laboratory tests were conducted on small single-edge notch specimens loaded in three-point bending to determine values for fracture toughness (KQ). Using the dimensions measured for each laboratory specimen, a J integral approach was employed to calculate KIC using finite element analysis. Both two-dimensional plane strain and three-dimensional models were used in determining KIC for each specimen, and these values were compared to the KQ values obtained from laboratory tests. The results indicated that no significant differences existed between laboratory results and those obtained from both two- and three-dimensional finite element models (P 〉 .85). For the three-dimensional model, values for KIC were found to vary across the specimen thickness, with the values at the center of the specimen closely paralleling those obtained from the two-dimensional plane strain J integral technique was as effective as the three-dimensional technique in calculating values for KIC. © 1995 John Wiley & Sons, Inc.
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