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  • Triticum aestivum  (153)
  • Angiosperms  (135)
  • Springer  (287)
  • American Meteorological Society
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 59-68 
    ISSN: 1573-0867
    Keywords: bicarbonate-extractable potassium ; muriate of potash ; potassium ; potassium chloride ; relative effectiveness ; silicate rock powder ; Triticum aestivum ; Trifolium subterraneum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Granite (silicate) rock dust, a by-product of quarry operations, is being advocated and used as a fertilizer in the wheatbelt of south-western Australia (WA). The dust is insoluble and based on its nutrient element content (1.9% K and 0.3%P and negligible N) it is not expected to be a useful fertilizer. Previous laboratory studies and glasshouse experiments in WA suggest the dust is a slow release K fertilizer. This paper extends the previous studies to consider the dust as an NP or K fertilizer in the year of application in a field experiment on a soil deficient in N, P and K. In addition, the effectiveness of the dust as a K fertilizer was compared with the effectiveness of KCl (muriate of potash), the K fertilizer used in WA at present, in glasshouse experiments using K deficient soils. In the field experiment, compared with NP fertilizer or NPK fertilizer (urea, supplying N; superphosphate, providing P, S, Ca, Cu, Zn and Mo; KCl providing K), the dust had no effect on grain yield of wheat (Triticum aestivum); in fact dust applied at 20 t ha-1, for unknown reasons, reduced yields by about 65% compared to the nil (no fertilizer, no dust) treatment. Relative to the nil treatment, applying NPK fertilizer increased yields about threefold, from 0.54 to 1.79 t ha. The glasshouse experiments showed that, relative to KCl, the dust was from about 0.02 to 14% as effective in K deficient grey sandy soils for producing dried tops of 30-day old wheat plants or 42-day old clover (Trifolium subterraneum) plants. In soils with adequate K (yellow sands, sandy loams or clays, loamy clays, clay loams and clays), neither KCl nor the dust affected yields of 30 to 42-day old wheat or clover plants grown in the glasshouse. In the glasshouse experiments, no yield depressions were measured for the dust applied up to 17 g dust per kg soil (equivalent to 17 t dust ha-1 mixed into the top 10 cm of soil in the field). It is concluded that the dust has no value as a fertilizer.
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  • 2
    Electronic Resource
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    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 117-123 
    ISSN: 1573-0867
    Keywords: labelled nitrogen ; Lolium perenne ; nitrogen cycling ; root biomass ; straw ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Large amounts of nitrogen (N) fertiliser (150–200 kg N/ha) are currently being applied to perennial ryegrass (Lolium perenneL.) seed crops in New Zealand. Due to increasing requirements for efficient use of N fertilisers and minimising nitrate contamination of the environment, a field experiment was established using 15N-labelled fertiliser to follow the fate of applied N. Urea-15N was applied to a perennial ryegrass seed crop in April (30 kg N/ha), August (30 kg N/ha), September (60 kg N/ha) and October (60 kg N/ha). The urea-15N was applied in solution and watered in to minimise volatilisation loss. At the time of harvest (December), 9% of the applied 15N was in the seed, 29% in the straw, 19% in the roots and 39% in the soil organic matter. Losses of 15N were minimal as the N was applied in several applications, each one at a relatively low rate, and at times when leaching was unlikely to occur. Ryegrass plants used a greater proportion of the N applied in September and October (61–65%) compared with that applied in April (44%). Consequently more N was recovered from the soil in the autumn application (57%) than from the September and October applications (28–44%). The availability of the residual fertiliser N to a subsequent wheat (Triticum aestivum L.) crop was studied in a glasshouse experiment. The residual fertiliser N was present in the soil and ryegrass roots and stubble. The wheat plants only recovered 7–9% of this residual N. Most of the N taken up by the wheat came from the soil organic N pool. Overall, applying a total of 180 kg N/ha to the ryegrass appeared to have minimal direct impact on the environment. In the short term N not used by the ryegrass plants contributed to the soil organic N pool.
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  • 3
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    Springer
    Sciences of soils 5 (2000), S. 10-21 
    ISSN: 1432-9492
    Keywords: Soil temperature ; Triticum aestivum ; Stubble retention ; Nitrogen ; Early growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Early growth and development are often lower when wheat is sown into standing stubble. A study was conducted to determine whether this difference in early growth could be explained by the effects of stubble on soil temperature in the vicinity of the young plant. The roles of nitrogen nutrition and soil strength were also assessed. Three crops were monitored (1990–1992), with the wheat being sown into either standing wheat stubble after a no-till fallow (NT), or into no-tilled plots from which the stubble had been removed by burning (NB). Measurements were made of wheat growth and development, soil and plant N, soil temperature and penetration resistance. The site was on a black earth near Warialda in the northern wheatbelt of New South Wales, Australia. In 1992 wheat was also grown under simulated stubble to isolate the shading and soil temperature effects of stubble from other factors. A significant (P〈0.05) relationship was found between average soil temperature and above ground dry matter (DM) at 65 days after sowing (DAS) but not at 107 DAS. This relationship accounted for differences in DM production at 65 DAS between NT and NB treatments in 1991 and 1992, but not in 1990. In that year the lower DM production in NT plots was associated with poorer N nutrition, and possibly disease. Laboratory incubations indicate that immobilisation of N as stubble decomposed could have contributed to this. Burning stubble produced no immediate increase in soil N availability, so that it is unlikely that N contained in stubble contributed to the difference. Soil strength differences between treatments and phytotoxic effects are unlikely to have contributed to growth differences in this soil.
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  • 4
    ISSN: 1432-2242
    Keywords: Key words Glutenin ; Triticum aestivum ; Wheat storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Low-molecular-weight (LMW) glutenin subunits consist mainly of two domains, one at the N- terminus which contains repeats of short amino-acid motifs, and a non-repetitive one rich in cysteine, at the C- terminal region. In previous reports, polyacrylamide-gel electrophoresis has been used to show that large size variation exists among LMW and HMW glutenin subunits, and it has been suggested that deletions and insertions within the repetitive region are responsible for these variations in length. In this study, PCR-amplification of genomic DNA (Triticum aestivum variety Chinese Spring) was used to isolate three full-length LMW glutenin genes: LMWG-MB1, LMWG-MB2 and LMWG-MB3. The deduced amino-acid sequences show a high similarity between these ORFs, and with those of other LMW glutenin genes. Comparisons indicate that LMWG-MB1 has probably lost a 12-bp fragment through deletion and that LMWG-MB1 and LMWG-MB2 have an insertion of 81 bp within the repetitive domain. The current study has shown direct evidence that insertions and/or deletions provide a mechanistic explanation for the allelic variation, and the resultant evolution, of prolamin genes. Single-base substitutions at identical sites generate stop codons in both LMWG-MB2 and LMWG-MB3 indicating that these clones are pseudogenes.
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  • 5
    ISSN: 1432-2242
    Keywords: Key words Wheat ; High-molecular-weight glutenin ; AS-PCR ; Glu-A1 locus ; Null allele ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The present work reports new PCR markers that amplify the complete coding sequence of the specific alleles of the high molecular weight (HMW) glutenin genes. A set of AS-PCR molecular markers was designed which use primers from nucleotide sequences of the Glu-A1 and Glu-D1 genes, making use of the minor diffeences between the sequences of the x1, x2* of Glu-A1, and the x5 and y10 of Glu-D1. These primers were able to distinguish between x2* and the x1 or xNull of Glu-A1. Also x5 was distinguishable from x2, and y10 from y12. The primers amplified the complete coding regions and corresponded to the upstream and downstream flanking positions of Glu-A1 and Glu-D1. Primers designed to amplify the Glu-A1 gene amplified a single product when used with genomic DNA of common wheats and the xNull allele of this gene. This work also describes the cloning and characterisation of the nucleotide sequence of this allele. It possesses the same general structure as x2* and x1 (previously determined) and differs from these alleles in the extension of the coding sequence for a presumptive mature protein with only 384 residues. This is due to the presence of a stop codon (TAA) 1215-bp downstream from the start codon. A further stop codon (TAG), 2280-bp downstream from the starting codon is also found. The open reading frame of xNull and x1 alleles has the same size in bp. Both are larger than x2* which shows two small deletions. The reduced size of the presumptive mature protein encoded by xNull could explain the negative effect of this allele on grain quality.
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  • 6
    ISSN: 1432-2242
    Keywords: Keywords AFLPs ; Bulked segregant analysis ; Marker-assisted selection ; Microsatellites ; Powdery mildew resistance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Molecular markers were identified in common wheat for the Pm24 locus conferring resistance to different isolates of the powdery mildew pathogen, Erysiphe graminis DM f. sp. tritici (Em. Marchal). Bulked segregant analysis was used to identify amplified fragment length polymorphism (AFLP) markers and microsatellite markers linked to the gene Pm24 in an F2 progeny from the cross Chinese Spring (susceptible)× Chiyacao (resistant). Two AFLP markers XACA/CTA-407 and XACA/CCG-420, and three microsatellite markers Xgwm106, Xgwm337 and Xgwm458, were mapped in coupling phase to the Pm24 locus. The AFLP marker locus XACA/CTA-407 co-segregated with the Pm24 gene, and XACA/CCG-420 mapped 4.5 cM from this gene. Another AFLP marker locus XAAT/CCA-346 co- segregated in repulsion phase with the Pm24 locus. Pm24 was mapped close to the centromere on the short arm of chromosome 1D, contrary to the previously reported location on chromosome 6D. Pm24 segregated independently of gene Pm22, also located on chromosome 1D. An allele of microsatellite locus Xgwm337 located 2.4±1.2 cM from Pm24 was shown to be diagnostic and therefore potentially useful for pyramiding two or more genes for powdery mildew resistance in a single genotype.
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  • 7
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    Theoretical and applied genetics 100 (2000), S. 32-38 
    ISSN: 1432-2242
    Keywords: Key words Waxy (Wx) protein ; Triticum aestivum ; Amylose content ; Starch ; Rapid Visco-Analyzer ; Swelling power ; Noodle quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Waxy (Wx) protein is a granule-bound starch synthase (GBSS) responsible for amylose production in cereal endosperm. Eight isolines of wheat (Triticum aestivum L.) having different combinations of presence and absence of three Wx proteins, Wx-A1, -B1, and -D1, were produced in order to elucidate the effect of Wx protein deficiencies on the apparent amylose content and starch-pasting properties. An improved SDS gel electrophoresis showed that ’Bai Huo’ (a parental wheat) carried a variant Wx-B1 protein from an allele, Wx-B1e. Thus, wheat lines of types 1, 2, 4, and 6 examined in this study contained a variant Wx-B1 allele and not the standard allele, Wx-B1a. The results from 3 years of experiments using 176 lines derived from two cross-combinations showed that apparent amylose content increased the least in type 8 (waxy) having no Wx proteins and, in ascending order, increased in type 5 (only the Wx-A1 protein is present) 〈type 7 (Wx-D1) 〈type 6 (Wx-B1) 〈type 3 (Wx-A1 and -D1) 〈type 4 (Wx-A1 and -B1) 〈type 2 (Wx-B1 and -D1) 〈type 1 (three Wx proteins). However, Tukey’ s studentized range test did not detect significant differences in some cases. Densitometric analysis suggested that the amylose content was related to the amount of the Wx protein in the eight types. Parameters in the Rapid Visco-Analyzer test and swelling power were correlated to amylose content. Consequently, amylose content and pasting properties of starch were determined to be influenced the most by the lack of the Wx-B1 protein, followed by a lack of Wx-D1, and leastly by the Wx-A1 deficiency, which indicated the presence of differential effects of the three null alleles for the Wx protein.
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  • 8
    ISSN: 1432-2242
    Keywords: Keywords Composite populations ; Triticum aestivum ; Blumeria (Erysiphe) graminis f. sp. tritici ; Residual resistance effects ; Quantitative resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The evolution of adult plant resistance towards powdery mildew (caused by Blumeria graminis f. sp. tritici) was investigated in 11 wheat populations cultivated for 10 years in a French network for dynamic management (DM) of wheat genetic resources. The aims of the study were to compare the evolution of resistance in sites submitted to different powdery mildew pressure and to investigate the implication of specific resistance gene action in adult plant resistance. For this, 7 of the 11 populations were characterized for their composition of specific resistance genes (results presented in a former paper). Even though no population differed significantly from the initial PA0 pool for mean adult plant resistance, divergence appeared among the final populations. The populations with the highest adult plant resistance level originated from sites where powdery mildew pressure is known to be high (Vervins, Le Rheu), whereas populations with the lowest adult plant resistance corresponded to areas with no, or very low, powdery mildew pressure (Toulouse, Montreuil-Bellay). A residual effect of defeated specific resistance genes was hypothesized, as lines accumulating at least two specific resistance genes appeared more resistant. Additional quantitative resistance seemed to be involved in adult plant resistance. DM lines appeared then as an interesting source of variability for resistance towards powdery mildew. Moreover, as these lines had been grown in mixed populations they may be appropriate as components of a composite cultivar.
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  • 9
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    Theoretical and applied genetics 100 (2000), S. 519-527 
    ISSN: 1432-2242
    Keywords: Key words Wheat ; Triticum aestivum ; Physical mapping ; Deletion lines ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Extended physical maps of chromosomes 6A, 6B and 6D of common wheat (Triticum aestivum L. em Thell., 2n=6x=42, AABBDD) were constructed with 107 DNA clones and 45 homoeologous group-6 deletion lines. Two-hundred and ten RFLP loci were mapped, including three orthologous loci with each of 34 clones, two orthologous loci with each of 31 clones, one locus with 40 clones, two paralogous loci with one clone, and four loci, including three orthologs and one paralog, with one clone. Fifty five, 74 and 81 loci were mapped in 6A, 6B and 6D, respectively. The linear orders of the mapped orthologous loci in 6A, 6B and 6D appear to be identical and 65 loci were placed on a group-6 consensus physical map. Comparison of the consensus physical map with eight linkage maps of homoeologous group-6 chromosomes from six Triticeaespecies disclosed that the linear orders of the loci on the maps are largely, if not entirely, conserved. The relative distributions of loci on the physical and linkage maps differ markedly, however. On most of the linkage maps, the loci are either distributed relatively evenly or clustered around the centromere. In contrast, approximately 90% of the loci on the three physical maps are located either in the distal one-half or the distal two-thirds of the six chromosome arms and most of the loci are clustered in two or three segments in each chromosome.
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  • 10
    ISSN: 1573-4927
    Keywords: nitrate reductase ; Triticum aestivum ; ditelosomics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The nitrate reductase activities (NRA) of 31 ditelosomic stocks were compared with that of the control plant [Chinese Spring (CS) euploid], using in vivo and in vitro assay procedures that had been optimized with respect to the euploid. Fourteen stocks exhibited significant differences in in vivo NRA from that of the euploid; the effect of removal of a chromosome arm was always to increase NRA. Eight of these stocks showed similar effects in vitro, although in three, a casein-sensitive factor had to be eliminated before the difference was expressed. Homoeologous group effects were evident among ditelosomics of groups 2, 4, and 7, while for three chromosomes (2D, 7A, and 7B), removal of either arm resulted in a similar increase in NRA in vivo and probably in vitro.
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  • 11
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    Theoretical and applied genetics 66 (1983), S. 101-109 
    ISSN: 1432-2242
    Keywords: Anther culture ; Culture temperature ; Induction frequency ; Pollen callus (plantlet) ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The response of anther culture to culture temperature was studied in detail using many varieties, F1 hybrids and pollen-derived lines of wheat (Triticum aestivum) as materials. The suitable culture temperature for inducing pollen callus (or embryoids) in wheat anther culture ranged from 26 °C to 30 °C, varying with genotypes. But for the great majority of wheat genotypes the suitable culture temperatures lay between 28 °C and 30°C. The most significant genotypic variation in the response to culture temperature was observed in the comparison between the culture at 33 °C for eight days followed by culture at 25 °C (or 26 °C) and the continuous culture at 25 °C (or 26 °C). This genotypic variation in the response to culture temperature is a heritable character which may be controlled by multiple genes. The effect of culture at 30 °C for eight days followed by culture at 26 °C was similar to, or in some cases, better than that of continuous culture at 28 °C, and the effect of culture at 32 °C for eight days followed by culture at 28 °C was similar to that of continuous culture at 30 °C. In the range from 26 °C to 32 °C, the overwhelming majority of pollen calli emerged before the 40th day after anther inoculation, and the higher the culture temperature, the earlier and more concentrated the emerging period of the pollen callus. The pollen callus obtained at high temperatures above 28 °C should be transferred in time onto the regeneration medium at 25°–27°C to induce shoots.
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  • 12
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    Theoretical and applied genetics 64 (1983), S. 255-258 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Malate dehydrogenase ; Chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The malate dehydrogenase (E.C. no 1.1.1. 37) of Triticum aestivum L. cv. Chinese Spring, shows two activity zones. The results obtained support the hypothesis that the malate dehydrogenase isozymes of zone II are dimers composed of the six possible combinations of subunits coded by triplicate genes located in the long arms of chromosomes of the homoeologous group 1.
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  • 13
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    Theoretical and applied genetics 68 (1984), S. 335-345 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; 2D electrophoresis ; Developmental genetics ; Cytoplasmic inheritance ; Genetic regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two-dimensional gel electrophoresis of denaturated proteins were performed at five developmental stages or organs (hereafter referred to as stage-organs) on two wheat lines with four different cytoplasms. Five hundred and fifty to 712 reproducible spots were scored depending on the stage-organ. Each stage-organ is unambiguously characterized and several types of control of protein quantity are recorded. Post-translational modifications are hypothetized and may sometimes be stagespecific. Two cytoplasmic patterns are found: one for the euplasmic lines with Triticum aestivum cytoplasm and one for the alloplasmic lines with Aegilops juvenalis, Ae. ventricosa and Ae. kotschyi cytoplasms. Cytoplasmic variation is observed for 28 spots showing position difference, all of which are probably products of the LS gene, and for four spots showing differences for regulation of protein quantity. Nuclear variation between ‘Chinese Spring’ and ‘Selkirk’ is found for 20 allelic differences and for 20 regulatory systems, the latter number being probably underestimated.
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  • 14
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    Theoretical and applied genetics 66 (1983), S. 111-121 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron ; Intergeneric hybrids ; Embryo culture ; Chromosome pairing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric hybrids of Triticum aestivum (2n=42,AABBDD) with Agropyron ciliare (2n= 28,SSYY), A. trachycaulum (2n=28,SSHH), A. yezoense (2n=28,SSYY) and A. scirpeum (2n=28) are reported for the first time. F1 hybrids of T. aestivum were also produced with A. intermedium (2n=42,E1E1E2E2Z1Z1) and A. junceum (2n=14,JuJu). All wheat-Agropyron hybrids were obtained by embryo rescue technique. Cultivars and reciprocal crosses differed for seed set, seed development and F1 plant production. The F1 hybrids were sterile. Attempts to obtain amphiploids were unsuccessful. However, backcross derivatives were obtained with wheat as the recurrent parent. The level of chromosome pairing in A. trachycaulum x wheat, A. yezoense x wheat and wheat x A. junceum hybrids provided no evidence of homologous or homoeologous pairing. Mean pairing frequencies in A. ciliare x wheat, wheat x A. scirpeum and wheat x A. intermedium hybrids indicated homoeologous or autosyndetic pairing. Ph gene was more effective in regulating homoeologous pairing in A. yezoense x wheat hybrids than in A. ciliare x wheat hybrid. Chromosome pairing data of BC1 derivatives indicated that either some of the wheat chromosomes were eliminated or Agropyron chromosomes caused reduced pairing of wheat homologues.
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  • 15
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Gliadins ; Electrophoresis ; Hybridological analysis ; Genetic nomenclature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inheritance of gliadin components in winter wheat has been studied by one-dimensional polyacrylamide gel electrophoresis. Single F2 grains from 36 intervarietal hybrid combinations have been analysed. The genetic analysis has revealed blocks, including 1–6 gliadin components, which are inherited as individual mendelian traits. About 80 variants of blocks have been detected. On the basis of the allelism test they are grouped into 6 series in accordance with the number of known gliadin-coding loci located on chromosomes of the homoeologous groups 1 and 6. Each series includes 8–18 blocks controlled by different alleles of one gliadin-coding locus. Blocks of components have been confirmed to be inherited codominantly in accordance to the gene dose in the triploid endosperm. The highest similarity between members of one series is observed in groups of blocks controlled by chromosomes ID and 6D. On the contrary, many blocks controlled by chromosomes 1A and 1B have no bands in common. The presented catalogue of blocks of components may be used to make up gliadin genetic formulae and to compare electrophoregrams obtained by different authors. Blocks of gliadin components are suitable genetic markers for use in revealing and studying heterogeneity of wheat varieties, in tracing their origin, in identifying recombinations, translocations and substitutions of the genetic material and in solving many other problems of the origin, evolution and selection of hexaploid wheat.
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  • 16
    ISSN: 1432-2242
    Keywords: Amphiplasty ; Ag-NOR ; Addition lines ; Nucleolar organizer ; Agropyron elongatum ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleolar organizer activity of the Agropyron elongatum, its amphiploid with hexaploid wheat (Triticum aestivum) and the chromosome addition lines is analyzed by the silver-staining procedure. Four Ag-NORs are observed in A. elongatum corresponding to the chromosomes 6E and 7E. In the amphiploid T. aestivum — A. elongatum, eight Ag-NORs are observed which corresponds the wheat chromosomes 1B and 6B and to the elongatum chromosomes 6E and 7E. Thus, there is codominance in the nucleolar organizer activity of the chromosomes of the two species. However, a partial amphiplasty is detected since less than 8 Ag-NORs (7 up to 4) are observed in some metaphase cells; the chromosomes 6E and 7E are occasionally suppressed by wheat chromosomes. This conclusion is confirmed by the behaviour of the addition lines since only in those corresponding to the chromosomes 6E and 7E are the elongatum chromosomes nucleolar active although occasionally they can be suppressed by wheat chromosomes.
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  • 17
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    Theoretical and applied genetics 69 (1984), S. 193-198 
    ISSN: 1432-2242
    Keywords: C-banding ; Heterochromatin ; Meiosis ; Common wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The meiotic identification of nine pairs of chromosomes at metaphase I of meiosis of Triticum aestivum (B genome, 4A and 7A) has been achieved using a Giemsa C-banding technique. As a result, the analysis of the pairing of each chromosome arm in disomic and monosomic intervarietal hybrids between ‘Chinese Spring’ and the Spanish cultivar ‘Pané 247’ could be carried out. Differences in the chiasmata frequencies per chromosome arm cannot be explained on the basis of relative arm lengths only. Possible effects of arm-to-arm heterochromatic differences on meiotic pairing are discussed.
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  • 18
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Gliadins ; Two-dimensional electrophoresis ; Hybridological analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Blocks of gliadin components found both in a number of varieties and in single F2 grains of winter wheat intervarietal hybrids have been studied by two-dimensional electrophoresis combining electrophoresis in acidic aluminium-lactate buffer (pH3.1) and SDS-electrophoresis. Gliadin components (spots) have been shown to be inherited as linked groups (blocks), codominantly and in accordance with a gene dosage in triploid endosperm. Blocks include components differing in their electrophoretic mobility and molecular weight. Some allelic variants of blocks differ only in presence of few additional components or in the electrophoretic mobility of components with similar molecular weights; other variants may contain no similar components. Apparently, in the course of evolution, mutations in individual genes of gliadin-coding loci and processes changing the number of expressing genes and the sizes of their structural part occurred.
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  • 19
    ISSN: 1432-2242
    Keywords: Key words Composite populations ; Triticum aestivum ; Blumeria (Erysiphe) graminis f. sp. tritici ; Selection ; Drift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Dynamic management has been proposed as a complementary strategy to gene banks for the conservation of genetic resources. The evolution of frequencies of genes for specific resistance towards powdery mildew (caused by Blumeria graminis f. sp. tritici) in populations of a French network for dynamic management of bread wheat genetic resources was investigated after 10 years of multiplication without human selection. The objective was to determine whether specific resistance gene diversity was maintained in the populations and whether any changes could be attributed to selection due to pathogen pressure. Seven populations, originating from four of the network sites, were characterized and compared to the initial population for six specific resistance gene frequencies detected by nine Blumeria graminis f. sp. tritici isolates. Diversity decreased at the population level, but because of a strong differentiation between the populations, this diversity was maintained at the network level. The comparison of Fst parameters estimated on neutral markers (RFLP) and on resistance gene data revealed that in two of the populations specific resistance genes had been selected by pathogen pressure, whereas evolution in two other populations seemed to be the result of genetic drift. For the three last populations, conclusions were less clear, as one had probably experienced a strong bottleneck and the other two presented intermediate Fst values. A dynamic management network with sites contrasted for pathogen pressure, allowing genetic drift in some populations and selection in others, appeared, at least on the short term, to be a good tool for maintaining the diversity of genes for specific resistance to powdery mildew.
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  • 20
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    Theoretical and applied genetics 65 (1983), S. 41-46 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Protein ; Mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Poor adaptability or functional quality of much germplasm used for breeding high-protein hard red winter wheats prompted mutagenesis as an alternative means of increasing grain protein content. Four hard red winter wheat genotypes — KS644 (‘Triumph// Concho/Triumph’), ‘Kaw’, ‘Parker’, and ‘Shawnee’ — were treated with 0.40 M ethyl methanesulfonate (EMS). Advanced lines (M8-M10) were selected that had a 3-year mean grain protein advantage of 0.7% to 2.0% over controls. Increased grain protein content was generally associated with decreased grain yield and kernel weight, but some high-protein mutant lines had yields or kernel weights similar to those of original genotypes. Changes in height and lodging induced by EMS were generally favorable, most mutants being shorter and lodging less than controls, but blooming date was generally delayed, a deleterious change. One line also changed from resistant to segregating for wheat soil-borne mosaic virus. Mutant lines might be utilized in cross-breeding programs, particularly if negative pleiotropic effects and linkages are absent.
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  • 21
    ISSN: 1432-2242
    Keywords: B-Chromosomes ; Chromosome associations ; Triticum aestivum ; Secale cereale hybrids
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    Notes: Summary T. aestivum var. ‘Chinese Spring’ (monosomic 5B and 5D, respectively) was crossed with S. cereale (with and without B-chromosomes). The resulting nullisomic 5B hybrids exhibited a high degree of chromosome association both at 20°C and 10°C. The presence of B-chromosomes reduced association slightly whether 5B was present or not. In nullisomic 5D hybrids B-chromosomes of rye raise chromosome association at 20°C when compared to hybrids with 5D, with as well as without, B's. At 10°C, due to the absence of the Ltp gene on 5D, chromosome association in nullisomic 5D hybrids is low, and no effects of rye B-chromosomes is detectable. The hypothesis that B-chromosomes of rye carry (an) asynaptic gene(s) decreasing effective pairing, and (an) independent post-synaptic gene(s) increasing chiasma frequency on effective pairing sites, is presented.
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  • 22
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    Theoretical and applied genetics 57 (1980), S. 57-58 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Anther culture ; Haploid
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    Topics: Biology
    Notes: Summary Five haploid plants of wheat were used for anther culture. Embryos were formed and six plants were regenerated. Of these, two were haploid (n=3x=21) and two diploid (2n=6x=42). The two diploids derived from the anthers of the same haploid wheat plant gave seeds, but the fertility was reduced in one of them showing, abnormalities at meiosis.
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  • 23
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    Theoretical and applied genetics 59 (1981), S. 153-160 
    ISSN: 1432-2242
    Keywords: Mitochondrial heterosis ; Mitochondrial complementation ; Triticum aestivum ; Hordeum vulgare ; Zea mays
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    Topics: Biology
    Notes: Summary Two families each of wheat (Triticum aestivum L.), barley (Hordeum vulgare L.) and maize (Zea mays L.) were studied for mitochondrial heterosis and in vitro mitochondrial complementation. Inbred parents and their hybrids were compared for seedling heights and rate of oxygen uptake by the whole tissue to find out if the hybrids showed greater growth and respiratory activity at the seedling stage. Further comparisons were made by isolating mitochondria from the seedling tissues and measuring their ADP∶0 ratio, respiratory control ratio and cytochrome c oxidase activity for mitochondrial heterosis. Mixtures of parental mitochondria were similarly compared with parental and hybrid mitochondria for in vitro mitochondrial complementation. No evidence for mitochondrial heterosis or in vitro mitochondrial complementation was found, nor any correlation between the different mitochondrial parameters, seedling heights or rates of oxygen uptake by seedling tissue. The suggested use of mitochondrial heterosis and in vitro mitochondrial complementation for plant breeding is discussed.
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  • 24
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    Theoretical and applied genetics 59 (1981), S. 313-316 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Puccinia graminis ; Stem rust resistance ; Monosomic analysis
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    Topics: Biology
    Notes: Summary Using monosomic analysis genes governing resistance in one wheat variety, ‘E5883’, against stem rust races 15C and 122, were located on specific chromosomes. Against race 15C, a major dominant gene, Sr6, was located on chromosome 2D of ‘E5883’ with minor modifiers found on chromosomes 4A and 6D. Against race 122, the presence of a recessive hemizygous ineffective gene, Sr8, was identified, located on chromosome 6A of variety ‘E5883’.
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  • 25
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Glutenin ; Gliadin ; Electrophoresis
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    Notes: Summary Subunits of wheat endosperm proteins have been fractionated by two-dimensional electrophoresis. To determine which subunits in the two-dimensional electrophoretic pattern belong to gliadin or glutenin the endosperm proteins have also been fractionated by a modified Osborne procedure and by gel filtration on Sephadex G-100 and Sepharose CL-4B prior to separation by two-dimensional electrophoresis. The control of production of five major grain protein subunits is shown to be determined by chromosomes 6A, 6B and 6D by comparing two-dimensional electrophoretic protein subunit patterns of aneuploid lines of the variety ‘Chinese Spring’. From these and previous studies it is concluded that some α, β and γ gliadins (molecular weights by SDS-PAGE 30,000 to 40,000) are specified by genes on the short arms of homoeologous Group 6 chromosomes, the ω gliadins (molecular weights by SDS-PAGE 50,000 to 70,000) are specified by genes on the short arms of homoeologous Group 1 chromosomes and the glutenin subunits (molecular weights by SDS-PAGE 〉 85,000) are specified by genes on the long arms of homoeologous Group 1 chromosomes. No major gliadins or glutenin subunits were absent when any of the chromosomes in homoeologous Groups 2, 3, 4, 5 or 7 were deleted. However two gliadins whose presumed structural genes are on chromosome 6D were absent in aneuploid stocks of ‘Chinese Spring’ carrying two additional doses of chromosome 2A. Two out of thirty-three intervarietal or interspecific chromosome substitution lines examined, involving homoeologous Group 2 chromosomes, lacked the same two gliadins. All the subunits in the other thirty-one chromosome substitution lines were indistinguishable from those in ‘Chinese Spring’. It is therefore concluded that the major variation affecting gliadin and glutenins in wheat is concentrated on the chromosomes of homoeologous Groups 1 and 6 but Group 2 chromosomes are candidates for further study. An endosperm protein controlled by chromosome 4D in ‘Chinese Spring’ is shown to be a high molecular weight globulin.
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  • 26
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat-phenolics-location
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    Notes: Summary Using thin-layer chromatography and nulli-tetrasomic and ditellosomic series of Triticum aestivum L. cv. ‘Chinese Spring’, it has been possible to relate the phenolic compounds found in adult plant leaves and 12 day-old seedling leaves with the chromosomes or chromosome arms 1 B, 2 BL, 3 BL, 5 A, 6 AL, 7 B and 7 DS.
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    Theoretical and applied genetics 63 (1982), S. 71-73 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Pollen ; Anther culture ; Callus ; Regeneration ; Plantlets
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    Topics: Biology
    Notes: Summary Pollen shed between 4–8 d from anthers of Triticum aestivum cultured in liquid medium gave rise to calluses. Tillers were harvested at the mid-to late-unicellular pollen stages and chilled for 8 d at 4–5 °C before the anthers were dissected out. Pollen cultures gave about 6 times as many calluses on a per anther basis as anthers cultured on solid medium. With the most productive of 5 cultivars tested, pollen culture results in roughly one callus for each anther used, though the calluses formed by pollen culture were less productive for the regeneration of shoots than calluses derived from anthers cultured on solid medium. The ratio of green to albino shoots is roughly 1∶ 1 for anther cultures but considerably less for pollen cultures.
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  • 28
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    Theoretical and applied genetics 63 (1982), S. 121-124 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Grainprotein ; Gene action ; Regression ; Diallel cross
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    Notes: Summary Diallel crosses between five divergent vulgare wheat cultivars were made in order to evaluate the mode of inheritance and combining ability of grain proteins. Significant differences in grain protein content were found between cultivars and their hybrids. It was established that the inheritance of seed protein in the F1 generation included both additive and non-additive gene action.
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  • 29
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    Theoretical and applied genetics 64 (1983), S. 97-101 
    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Electrophoresis ; Endosperm proteins
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    Topics: Biology
    Notes: Summary Total endosperm protein subunits, extracted from the common wheat cultivar Chinese Spring and from some of its aneuploid lines, were fractionated according to their molecular weight (MW) in an improved high resolution one-dimensional sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). The resolution obtained by this method and, in particular, that of the high molecular weight (HMW) glutenin and gliadin subunits approached that of a previous report in which two-dimensional fractionation system based on charge and MW was used. In the cultivar Chinese Spring, 21 discrete protein bands were resolved and the chromosomes controlling many of them were either reconfirmed, or, in some cases, established. The advantages of this high resolution SDS PAGE technique are discussed.
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    Theoretical and applied genetics 67 (1983), S. 53-58 
    ISSN: 1432-2242
    Keywords: Aegilops umbellulata ; Genetics ; Lectin ; Triticum aestivum
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    Topics: Biology
    Notes: Summary Each of the three genomes in hexaploid wheat controls the expression of a specific lectin in the embryo. The chromosomes which control their synthesis were determined using nullisomic-tetrasomic and inter-varietal chromosome substitution lines of ‘Chinese Spring’. All three wheat lectins were shown to be controlled by the homoeologous group 1 chromosomes. Using ditelosomic lines of ‘Chinese Spring’ the lectin genes could be localized on the long arms of chromosomes 1A and 1D. Inter-specific addition and substitution lines of Aegilops umbellulata chromosomes to ‘Chinese Spring’ indicated that chromosome 1U, which is homoeologous to the group 1 chromosomes of wheat, controls lectin synthesis.
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  • 31
    ISSN: 1432-2242
    Keywords: HMW glutenin subunit genes ; cDNA clones ; Tandem DNA repeats ; Chromosomal location ; Gene copy number ; Wheat ; Triticum aestivum
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    Topics: Biology
    Notes: Summary cDNA clones encoding wheat HMW glutenin subunits have been isolated from a cDNA bank made to poly A+ RNA from developing wheat endosperm var. Chinese Spring. One such clone, pTag 1290, has enabled us to identify the HMW glutenin mRNA species. The DNA sequence of this clone has been partially determined and it contains several tandem DNA repeats. The sequence is discussed in relation to the generation of the HMW glutenin subunit gene family. Analysis of the organization of the HMW glutenin sequences in the wheat genome revealed that the genes encoding HMW glutenin subunits exist in low copy number and are located on the long arm of each of the homoeologous group 1 chromosomes.
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  • 32
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; T. timopheevii ; Wheat ; Photoperiod ; Vernalization ; Male sterility ; Alloplasmic hybrids
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    Notes: Summary Studies were conducted to determine the influence of the male sterility-inducing cytoplasm of Triticum timopheevii (Zhuk.) Zhuk. on response of several common winter wheat (T. aestivum L.) nuclear genotypes to photoperiod and vernalization. Comparative studies of cytoplasmic substitution lines provide information on the role of the cytoplasmic genetic mechanism in growth and development. In the case of cytoplasmic male sterility-based hybrid production systems, ubiquity of sterility-inducing cytoplasm in derived hybrids warrants thorough characterization of its influence on plant phenotype. Factorial combinations of cytoplasm (T. timopheevii and T. aestivum), nuclear genotype, and photoperiod or vernalization treatments were evaluated under hydroponic conditions in controlled environment chambers. Interaction of cytoplasm, photoperiod, and nuclear genotype was significant in one or more experiments for days to anthesis and potential spikelet number, and interaction of cytoplasm, vernalization, and nuclear genotype was significant for days to spike emergence. Long day length was associated with increased percentage seed set in one study, but interactions of photoperiod and cytoplasm were not detected for percentage seed set. Interactions involving cytoplasm and photoperiod or vernalization were interpreted as evidence of the existence of genetic factors in cytoplsam of T. timopheevii which alter photoperiod or vernalization responses of alloplasmic plants relative to responses exhibited by euplasmic plants. Since photoperiod and vernalization responses are critical to adaptation, T. timopheevii cytoplasm can alter adaptability of T. aestivum. The specific effect would be nuclear genotype dependent, and does not appear to be of a magnitude greater than that induced by nuclear genetic variability at loci conditioning photoperiod or vernalization responses or other adaptation-determining characteristics. Normal multilocation/year testing of alloplasmic hybrids should therefore adequately identify zones of adaptation.
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    Theoretical and applied genetics 66 (1983), S. 77-86 
    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Electrophoresis ; Endosperm proteins ; Glutenins ; Gliadins
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    Notes: Summary Endosperm protein subunits of 109 primitive and modern lines of hexaploid wheat, Triticum aestivum L. em. Thell., were fractionated by one-dimensional, high resolution, sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). A wide range of both qualitative and quantitative variation was observed in the fractions of the high molecular weight (HMW) glutenin and gliadin subunits of the different lines. The qualitative variation was expressed in the number of subunits per fraction and in their molecular weight, as determined by the differential rate of migration. The quantitative variation was expressed in the differential staining intensity of several subunits. The widest variation was detected in the HMW glutenin and gliadin subunits controlled by chromosome 1B while a much smaller variation was observed in those subunits controlled by chromosome 1A and further smaller variation in the subunits controlled by 1D. Only a small number of subunits in both fractions was found to be controlled by chromosome 1A indicating that diploidization of endosperm protein genes in common wheat has been non-random. The genetic and evolutionary implications of these findings are discussed.
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  • 34
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    Theoretical and applied genetics 57 (1980), S. 57-58 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Anther culture ; Haploid
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    Topics: Biology
    Notes: Summary Five haploid plants of wheat were used for anther culture. Embryos were formed and six plants were regenerated. Of these, two were haploid (n=3x=21) and two diploid (2n=6x=42). The two diploids derived from the anthers of the same haploid wheat plant gave seeds, but the fertility was reduced in one of them showing, abnormalities at meiosis.
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  • 35
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    Theoretical and applied genetics 58 (1980), S. 247-252 
    ISSN: 1432-2242
    Keywords: Maternal tissues ; Grain weight ; Wheat ; Triticum aestivum ; Caryopsis ; Pericarp ; Endosperm ; Embryo
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    Topics: Biology
    Notes: Summary Reciprocal crosses were made between semi dwarf spring wheat cultivars (Triticum aestivum L.) differing in grain weight. The weights of the F1 grains (on maternal spikes), from intact as well as from defoliated plants, and those of the F2 grains (on f1 spikes), were examined. Grain weight was controlled primarily by the genotype of the maternal tissues (pericarp, testa or other floret or spikelet organs, including the vascular system), with complete dominance of heaviness. No indications suggesting maternal inheritance were obtained. The frequency distribution of the weights of the F2 grains indicated the presence of genotypic effects exerted by the endosperm or embryo. The embryo or endosperm factors for heaviness also seemed to be dominant.
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    Theoretical and applied genetics 60 (1981), S. 157-160 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Puccinia graminis ; Stem rust resistance ; Inheritance
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    Topics: Biology
    Notes: Summary Two bread wheat accessions, E5008 and E6160, have been genetically analysed for resistance genes effective against Indian cultures of stem rust races, 15C and 122. The inheritance of resistance to each race has been determined from the F1 and F2 of the crosses (resistant parents with the susceptible variety, ‘Agra Local’) and F2 progenies from the backcross to ‘Agra Local’. Tests have been performed to see if the two varieties carry common genes/s for resistance. The identity of the genes for resistance has been established from relevant crosses with single gene lines carrying known genes for resistance. A single dominant gene effective to race 15C in E5008 has been demonstrated to be Sr9b. Of the two recessive genes, each producing distinct infection types (0; and 1–3) against race 122, one gene has been inferred to be Sr12 and the second to be a hitherto undesignated gene. The resistance of E6160 against race 15C is controlled by two genes, one dominant and one recessive. The dominant gene has been identified as Sr9b. The recessive gene has been inferred to be a new gene. Similarly, a dominant gene effective against race 122 in E6160 has been observed to be different from those so far designated. In addition, the presence of modifier gene/s in the variety, E6160 has been suggested.
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    Theoretical and applied genetics 61 (1982), S. 129-133 
    ISSN: 1432-2242
    Keywords: Interchange ; Chromosome arrangements ; Common wheat ; Triticum aestivum
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    Topics: Biology
    Notes: Summary Chromosome arrangements of twenty-eight cultivars of common wheat, Triticum aestivum L., from or introduced into Spain are compared with that of ‘Chinese Spring’ taken as a pattern. All the cultivars analyzed differ from ‘Chinese Spring’ by one or two reciprocal translocations. When 12 out of 28 cultivars were compared it was concluded that a minimum number of thirteen interchanges are present, involving at least ten different chromosomes of the complement. The interest of a reappraisal of the rôle of interchanges in the evolution of Gramineae is pointed out.
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    Theoretical and applied genetics 61 (1982), S. 337-339 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; ‘Chinese Spring’ ; Chromosome banding ; Hydrolysis ; Feulgen
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    Notes: Summary Terminal and/or interstitial N-bands were produced on the seven B-genome chromosomes and chromosomes 4 and 7 of the A-genome of Triticum aestivum cv. ‘Chinese Spring’ by a modified BSG technique following a standard Feulgen preparation. The banding was accomplished by modifying the barium hydroxide treatment.
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  • 39
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    Theoretical and applied genetics 66 (1983), S. 1-7 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Aegilops species ; Alloplasmic lines ; 2D gel electrophoresis ; Cytoplasmic inheritance
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    Topics: Biology
    Notes: Summary In this first analysis the protein patterns obtained by two-dimensional gel electrophoresis of 8 day-old leaves from 18 alloplasmic wheat lines are compared. From 440 spots retained on the basis of their reproducibility, 36 proteins were observed to vary in different cytoplasms, allowing us to distinguish the T. aestivum cytoplasm from 5 Aegilops cytoplasms. Twenty-four of the 36 variable proteins could be structurally related to the large subunit of RuBPCase. Nuclear variation between 3 wheat varieties was observed for 14 proteins.
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    Plant systematics and evolution 134 (1980), S. 11-21 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Daucus ; Petroselinum ; Acer ; rRNA ; transcription ; precursors of less than 2.5 × 106 daltons ; processing
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    Topics: Biology
    Notes: Abstract Actively dividing cells from parsley (Petroselinum crispum) and carrot (Daucus carota) (bothApiaceae) andAcer pseudoplatanus (Aceraceae) were used to detect the primary gene product of the rDNA in plant cells. Parsley and carrot cells were labelled with [32P] orthophosphate. In both cases only one high molecular weight rRNA precursor was present on polyacrylamide gels under non-denaturing conditions. Its molecular weight did not exceed 2.5 × 106 daltons. The component emerged from the heterogenous material after a labelling period of 5–10 min. In parsley cells 45 min after onset of incubation labelled mature rRNA (25S and 18S) arrived in the cytoplasm. InAcer pseudoplatanus (incubation period 60 min) two rapidly labelled components did emerge from polyacrylamide gels; their molecular weights were 2.3 and 3.2− 3.4 × 106 daltons. After electrophoresis under denaturing conditions the larger component was no longer present, thus indicating that it was an aggregate of different RNA molecules. The molecular weights of the rRNA precursors ofD. carota andP. crispum determined after electrophoresis in formamide gels were about 2.1 × 106 daltons. From these results we have no evidence for the existence of rRNA precursors exceeding the molecular weight of 2.5 × 106 daltons.
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    Plant systematics and evolution 135 (1980), S. 11-39 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Winteraceae ; Drimys brasiliensis ; Development of inflorescences ; flowers and fruits ; reproductive biology ; pollination ; seed dispersal ; vegetative propagation ; early Angiosperm evolution
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    Topics: Biology
    Notes: Abstract From field observations onDrimys brasiliensis, principally in the Botucatu region of São Paulo State, Brazil, new data on the reproductive biology, the rhythm of growth, and the development of lateral cymose inflorescences, flowers and fruits are presented. Pollination accelerates the rate of flower-development for about 4–6 days. Pollination experiments show thatD. brasiliensis is not self-sterile; because of mechanical devices the sticky pollen grains do not normally come into contact with the stigmata unless an animal pollen vector is involved. The pollinators are diurnalColeoptera, Diptera andThysanoptera which eat from the pollen, lick from the stigmatic exudates and (in case of the flies) probably also from the staminal glands. Fruit- and seedeaters are birds which seem to be the main dispersal agents. Establishment of new individuals normally is through seedlings, but also by vegetative propagation through plagiotropous branches which may root and separate from the mother plant. The morphological, developmental and reproductive aspects inD. brasiliensis are discussed in a wider context, compared with data from otherMagnoliidae, and related to aspects of early Angiosperm evolution.
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    Plant systematics and evolution 137 (1981), S. 73-94 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Brassicaceae ; Capsella bursa-pastoris ; Isoenzyme polymorphism ; population analyses ; geographic variation ; heterozygosity ; breeding system
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    Topics: Biology
    Notes: Abstract The genetic variability of natural populations ofCapsella bursapastoris in North- and Middle-Europe has been estimated by means of enzyme assays. Zymograms of 81 populations have been developed. 17 loci could be identified, and 8 of them can be heterozygous. Genetic variability is greater between populations than within. No correlation between actual population sizes and genetic heterogeneity could be detected. Some electromorphs shift their frequencies proportionally to increasing adversity of climatic conditions, some appear to be constant over the whole area, and others are characterized by an apparently irregular variation pattern. Marginal populations comprise a significantly higher proportion of heterozygous plants than central ones. Apart from this clinal variation pattern, a mosaic pattern, strongly related to habitat conditions, was observed: genetic heterogeneity is greater in more intensively disturbed sites. The pattern of genetic variation in natural populations ofCapsella bursa-pastoris is rather highly influenced by the breeding system.
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    Plant systematics and evolution 136 (1980), S. 247-258 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae (=Gramineae) ; Triticum ; Aegilops ; diploid species ; Starch Gel electrophoresis ; allozymic variation ; phylogenetic relationships ; evolution
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    Topics: Biology
    Notes: Abstract Twenty enzyme loci were examined in the diploid species ofTriticum andAegilops for allelic variation by starch gel electrophoresis. SectionSitopsis, including the five species,Ae. speltoides, Ae. lingissima, Ae. sharonensis, Ae. bicornis andAe. searsii form a close subgroup withAe. speltoides slightly removed from the others.T. monococcum s. lat., was found to be closest to the species of theSitopsis group.Ae. comosa, Ae. umbellulata andAe. uniaristata form a second subgroup withAe. caudata most closely related to these species.Ae. squarrosa appears almost equally related to all of the species, showing no special affinity for any one species group. Nineteen out of twenty loci examined were polymorphic with a mean of 6.7 alleles per locus. Species could be, for most loci, characterized by the presence of predominant alleles. A conspicious genetic characteristic ofTriticum-Aegilops is the sharing of these predominant alleles between species. Within species variation is characterized by a diffuse distribution of secondary alleles.
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    Plant systematics and evolution 137 (1981), S. 63-65 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Emblingia ; Emblingiaceae ; Sapindaceae ; Dodonaea ; Stylobasidaceae ; Stylobasium ; Gyrostemonaceae ; Anemophily ; entomophily ; autogamy
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    Topics: Biology
    Notes: Abstract Emblingia is a short lived fire weed with a highly autogamous breeding system.
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    Plant systematics and evolution 138 (1981), S. 47-60 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Ranunculaceae ; Ranunculus seguieri ; R. alpestris ; R. traunfellneri ; R. bilobus ; R. crenatus ; Relationship betweenR. seguieri andR. alpestris s. lat.: morphology ; crossing experiments ; chromosome numbers ; karyotypes
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    Topics: Biology
    Notes: Abstract Morphological and cytological investigations as well as crossing experiments were carried out withRanunculus seguieri Vill. and 4 species of theRanunculus alpestris L. group (R. alpestris L.,R. traunfellneri Hoppe,R. bilobus Bertol.,R. crenatus Waldst. & Kit.). ForR. seguieri andR. alpestris, localities and distribution are given in addition to extensive diagnoses. A key to the species includes morphological characteristics and distribution data forR. traunfellneri, R. bilobus, andR. crenatus. New diagnostic characters are described. Crossing experiments betweenR. seguieri and the species of theR. alpestris group were unsuccessful. All 5 species have a chromosome number of 2n = 16, the record forR. bilobus is new. There is no statistically significant difference between the karyotypes ofR. seguieri andR. alpestris s. str. Nevertheless, according to morphological evidence and crossing experiments,R. seguieri is not closely related to theR. alpestris group.
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    Plant systematics and evolution 137 (1981), S. 127-134 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Boraginaceae ; Pyrrolizidine alkaloids ; phenolic compounds ; chemotaxonomy
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    Topics: Biology
    Notes: Abstract By means of thin layer chromatography pyrrolizidine alkaloids and phenolic compounds in some members of the familyBoraginaceae, subfamiliesHeliotropioideae andBoraginoideae, were studied. FromOmphalodes verna a main alkaloid was isolated with an isoretronecanol (or stereoisomeric) nucleus. The chemotaxonomical model ofTétényi forBoraginaceae based on fatty acids is generally supported, but relationships betweenHeliotropioideae andCynoglosseae appear to be closer, suggesting parallel developments from common ancesters.
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    Plant systematics and evolution 138 (1981), S. 175-188 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Eriocaulaceae ; Eriocaulon setaceum ; Embryology ; systematics
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    Notes: Abstract Eriocaulon setaceum can be characterized by: young microsporangium wall with epidermis, endothecium (with fibrous thickenings), and glandular tapetum (uninucleate cells); pollen grains 3-celled, spiraperturate; embryo sac development according to the Polygonum type and with antipodal cyst; endosperm nuclear; embryo small, with incipient differentiation into cotyledonary and epicotylary loci; seed coat mainly from the inner layers of the integuments; pericarp 2-layered and membranous. Embryologically, theEriocaulaceae are nearer to theXyridaceae than to otherFarinosae. Their elevation to the rank of an order,Eriocaulales, therefore appears justified.
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  • 48
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    Keywords: Angiosperms ; Poaceae ; Hordeum spontaneum ; Allozymes ; microgeographic differentiation ; population genetics
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    Notes: Abstract Allozymic variation in proteins encoded by 22 loci was analyzed electrophoretically in 278 individual plants of wild barley,Hordeum spontaneum, the progenitor of cultivated barley, in four 100 meter transects, in Israel, each equally subdivided into basalt and terra rossa soil types. Significant differentiation according to soil was found in 9 alleles. Our results suggest that allozyme polymorphisms in wild barley are at least partly adaptive, and differentiate by edaphic natural selection rather than by stochastic processes, and/or neutrality of allozymic variants.
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    Plant systematics and evolution 139 (1981), S. 11-45 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Solanaceae ; Lycopersicon ; Solanum pennellii ; Allozymes ; electrophoresis ; isozymes ; mating systems ; self-incompatibility
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    Notes: Abstract Genetic variation—primarily in 19 genetic loci of seven enzyme systems—was analyzed in accessions from various parts of the geographic range ofSolanum pennellii, which according to all tested biosystematic criteria behaves like a species ofLycopersicon. In comparison with the largely sympatricL. hirsutum andL. pimpinellifolium, this species exhibits the same trends of reduced allogamy and decreased genetic variation toward the north and south margins of its distribution, though to a much lesser degree; it does not exhibit their trends toward smaller flower size in the same peripheral regions. All three species agree to a considerable extent in the ranking of their tested loci in respect to degree of variablility; however, overall polymorphy is highest inS. pennellii. Except for the appearance of self-compatibility at its southernmost margin,S. pennellii is exclusively and rigidly self-incompatible. Alleles are distributed much more uniformly over the range than in the previously mentioned species, marginal and internal endemic mutants being much less abundant. A marked geographic disagreement is evident in regions of high and low variation. These differences in patterns of genetic variability are reconciled in terms of observed differences in mating systems, probable age of distributions, and adaptive strategies.
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    Plant systematics and evolution 138 (1981), S. 259-262 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Bromeliaceae ; Tillandsia subgen.Allardtia ; T. kolbii ; spec. nova ; Flora of Mexico
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    Notes: Abstract A new species ofTillandsia is described and discussed in regard to related species.
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    Plant systematics and evolution 138 (1981), S. 263-274 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rutaceae ; Citrus limon ; Style ; canal cells ; canal filling substance ; ultrastructure ; anatomy
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    Notes: Abstract The ultrastructure of the canal cells and the canal filling substance ofCitrus limon have been studied. At maturity the canal cells are very rich in cytoplasm. Their inner tangential walls lining the canal are much thickened and formed by two layers: the outer corresponds to the original wall, the inner is formed by subsequent deposition of abundant materials of different origin. This thickening occurs at the same time as the filling of the stylar canal. Both events are paralleled by considerable dictyosomic activity, the formation of a large amount of rough endoplasmic reticulum, and the incorporation of small cytoplasmic masses into the cell wall, due to plasmalemma evaginations. — The material in the stylar canal has a heterogeneous ultrastructure aspect and consists of polysaccharides, proteins and lipids; it presumably provides nutrients for the growing pollen tubes.
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    Plant systematics and evolution 139 (1982), S. 209-227 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Dicotyledons ; Monocotyledons ; DNA reassociation kinetics ; fast repeats ; repetitive DNA ; single copy DNA ; short period interspersion pattern ; slow repeats ; genome evolution
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    Notes: Abstract Angiosperms investigated by DNA/DNA reassociation studies were classified and tested for a taxonomic class- and subclass-specifity in a biometrical fashion. Monocotyledons and Dicotyledons differ significantly from each other with respect to a genomic parameter (U/R-ratio;U single copy DNA fraction;R = 1-U fraction of repetitive DNA). This difference is discussed from an evolutionary and molecular point of view.—Intercorrelations between the fraction of fast repeats, slow repeats, and single copy DNA can be detected. The amount of DNA organized in a short period pattern of interspersion is found to depend on the fraction of repetitive and single copy DNA. The number of DNA segments tandemly arranged in a short period pattern is linearly correlated withR/U-values. This correlation allows for a formula suitable for the estimation of the number of active genes in angiosperms. The analytical complexities of repetitive and single copy DNA are linearly correlated with the genome size of higher plants. The ratioU/R depends on the genome size of angiosperms in a hyperbolic fashion.
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    Plant systematics and evolution 139 (1982), S. 163-178 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Liliaceae ; Tulipa subg.Leiostemones ; Giemsa banding ; heterochromatin ; karyosystematics
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    Notes: Abstract The chromosomes of several cultivatedTulipa species of subg.Leiostemones were examined in conventionally stained and C-banded preparations. The heterochromatin content varied from almost none to 45%. Several chromosome types were recognized with respect to chromosome morphology and heterochromatin distribution, and groups of species with common chromosome characteristics could be identified. These karyological relationships are discussed with respect to the groups formed on the basis of floral and bulb charateristics.
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    Plant systematics and evolution 137 (1981), S. 145-180 
    ISSN: 1615-6110
    Keywords: Coniferophytina ; Angiosperms ; Arecaceae ; Poaceae ; Quercus ; Citrus ; sclerophyllous trees ; mountain plants ; Acclimatation ; chilling resistance ; frost resistance ; stress ecotypes
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    Notes: Abstract Gradations in cold resistance of plants with different latitudinal and altitudinal distribution, and the various categories of chilling and freezing resistance are hypothesized to be evolutionary steps of adaptive responses to increasing low temperature stress and annual seasonality. The gradual lowering of the critical phase transition temperature of biomembrane lipids, the capacity of persistent supercooling of tissues, and the development of a dormancy linked freezing-tolerance are considered to be essential mechanisms resulting in improved acclimatation to low temperature climates.
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    Plant systematics and evolution 138 (1981), S. 89-113 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Nicotiana ; Gossypium ; Lemnaceae ; Fraction 1 protein ; differentiation and age of taxa ; migration to Australia ; land connections ; long distance dispersal
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    Notes: Abstract Fraction 1 protein (F-1-protein) (ribulose bisphosphate carboxy-lase-oxygenase) contained inLemnaceae has been evolving for at least 50 million years because fossils of these plants have been identified in strata belonging to the Upper Cretaceous. Electrofocusing F-1-protein resolves the large subunit polypeptides coded by extranuclear DNA and the small subunit polypeptides coded by nuclear DNA. Four differences affecting isoelectric points of the large subunit polypeptides and eight affecting the small subunit polypeptides are now present among eleven species representing the four genera comprising theLemnaceae. In comparison, four differences in the large and 13 in the small subunit polypeptides exist among 63 species ofNicotiana; four differences in the large and eight differences in the small subunit polypeptides exist among 19 species ofGossypium. The number of differences in F-1-protein composition being of the same order of magnitude for the generaNicotiana, Gossypium, and the familyLemnaceae, we infer that these Angiosperms are of similar antiquity. Nicotiana species indigenous to Australia and Africa contain F-1-proteins whose large subunit polypeptides are different but some of whose small subunit polypeptides are like those found in species from the Western Hemisphere. The same situation is found for the F-1-protein inGossypium. These results are in harmony with the view that species ofNicotiana andGossypium have arrived in Australia via former land connections between S. America, Antarctica, and Australia.
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    Plant systematics and evolution 138 (1981), S. 139-146 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Magnoliidae ; Lauraceae ; Hernandiaceae ; Pollen morphology ; pollen ultrastructure ; Angiosperm phylogeny
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    Notes: Abstract The pollen grains ofLauraceae andHernandiaceae are characteristic in having a tubular exine of ± microfibrillar structure. This is coated with a layer of medium electron-dense globules and very peculiar spines, made up of a substance differing from that of the exine. Such a pollen wall structure is otherwise unknown in seed plants and thus adds to the list of odd features erratically present in various taxa of the Ranalean complex.
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    Plant systematics and evolution 138 (1981), S. 189-198 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Malvaceae ; Hibiscus acetosella ; H. asper ; H. surattensis ; H. radiatus ; andH. cannabinus ; Allopolyploidy ; evolution of dosage compensation ; alcohol dehydrogenase ; malate dehydrogenase ; leucine aminopeptidase
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    Notes: Abstract Mature seeds of diploid and tetraploidHibiscus species were analyzed for enzyme activity (alcohol dehydrogenase, malate dehydrogenase, leucine aminopeptidase), total protein content, DNA amount and dry weight. The recently formed tetraploid,H. radiatus, generally had enzyme and protein levels very similar to the sum of its progenitors, while the more ancient speciesH. acetosella had several lower levels. This difference may reflect the greater amount of timeH. acetosella has had to evolve dosage compensations.
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    Plant systematics and evolution 138 (1981), S. 235-246 
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    Keywords: Angiosperms ; Asteraceae ; Lactuceae ; Microseris ; Canalized numbers ; quantitative genetics ; evolutionary genetics ; pappus parts
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    Notes: Abstract The ChileanMicroseris pygmaea has a ten-part paleaceous pappus while the CalifornianM. bigelovii has five pappus parts on each achene. Hybrids between the two species have between five and ten pappus parts with averages below 7.5. Hybrid B 87 has an F 1 average value of 6.7 pappus parts. 140 F 2 plants were raised from this hybrid, and 12 F 3 families were obtained by selfing from F 2 plants. One larger F 4 family has been raised. Pappus part number in all of these is still canalized between 5 and 10. Variation within these limits is genetically determined by a quantitatively acting polygenic system. Modeling of this system suggests that a minimum of four, but probably not many more, genes are involved. This opens the possibility of a complete genetic analysis of the system.
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    Plant systematics and evolution 139 (1981), S. 95-102 
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    Keywords: Angiosperms ; Menispermaceae ; Cocculus hirsutus ; Seed ; fruit ; anatomy ; affinities
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    Notes: Abstract The ovules ofCocculus hirsutus are anatropous, bitegmic and, crassinucellate. The fruit is drupaceous, black purple, and laterally compressed and has a pericarp demarcated into exocarp and endocarp. The seeds are curved around the basal bodies. Testa and tegmen cells are thin-walled and unspecialized. In the ripe seed the inner epidermis of the tegmen persists, whereas the entire testa and the outer layer of the tegmen degenerate. Relationships of theMenispermaceae toRanunculaceae, Berberidaceae, andLardizabalaceae are supported.
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    Plant systematics and evolution 139 (1981), S. 103-111 
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    Keywords: Angiosperms ; Rubiaceae ; Nodal anatomy ; leaf base ; stipules
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    Notes: Abstract The leaf base vascularization in most of theRubiaceae corresponds to the unilacunar pattern with one complex trace. Only some species are known to have a trilacunar nodal pattern with three traces. In the unilacunar type one pair of smaller bundles separates from the complex lacunary leaf trace laterally, each soon forking into two arms: One arm becomes a marginal vein of the petiole, the other, besides supplying the stipules, forms a ± distinct vascular “bridge” within the cortex of the nodal flanks. In theRubieae this flank bridge develops as a very distinct vascular “ring” commissure out of which the whorled leaf-like appendages are vascularized; only the opposite true leaves receive their complex trace out of the lacunes directly. Axillary branches originate only from these true leaves.
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    Plant systematics and evolution 140 (1982), S. 39-55 
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    Keywords: Angiosperms ; Brassicaceae (Cruciferae) ; Alysseae ; Arabideae ; Brassiceae ; Heliophileae ; Hesperideae ; Lepidieae ; Pringleeae ; Sisymbrieae.—Serological systematics
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    Notes: Abstract The serological investigations support the opinion ofJanchen (1942) to combine the generaBunias, Isatis, andSisymbrium in the tribeSisymbrieae; Cheiranthus, Erysimum, andMatthiola in the tribeHesperideae; andBrassica, Crambe, Sinapis, andSuccowia in the tribeBrassiceae. They further underline the central position of theSisymbrieae and the isolated position of theHeliophileae. In accordance withEigner (1973) theBrassiceae are placed closer to theSisymbrieae than inJanchen; the same holds for thePringleeae. No serological justification could be found to uniteArabis andBarbarea in the tribeArabideae, andAlyssum andLunaria in theAlysseae. From the antigen-systems used among the representatives ofJanchen's Lepidieae the generaLepidium andNeslia show remarkable correspondence both toCamelina andThlaspi, but not toCochlearia which appears distant fromCamelina andThlaspi also.
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    Plant systematics and evolution 140 (1982), S. 109-117 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Gramineae (Poaceae) ; Agrostis tenuis.—Evolution ; Copper tolerance ; genotype-environment interaction
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    Notes: Abstract The effect of increased copper concentration in solution on the rooting of seven copper tolerant populations ofAgrostis tenuis has been examined using the regression technique ofFinlay &Wilkinson (1963). Three types of response to increased copper concentration have been detected, and it is suggested that these reflect a different genetic control of copper tolerance in different populations.
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    Keywords: Angiosperms ; Boraginaceae ; Symphytum.—Pyrrolizidine alkaloids ; chemotaxonomy
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    Notes: Abstract By means of thin layer chromatography in conjunction with mass spectrometry the pyrrolizidine alkaloid patterns derived fromSymphytum asperum, several cytotypes ofS. officinale agg. and the artificial hybrids of the former taxa, were compared. The obtained patterns were not essentially affected by variation in cytotype, harvesting times and -location of plants. Lycopsamine, acetyl-lycopsamine and symphytine or their isomers were generally found in theS. officinale cytotypes, echimidine and symphytine inS. asperum. The interspecific hybrids contained all alkaloids mentioned. The definite lack of echimidine in the 2 n=40 cytotype proves that it is conspecific withS. officinale and does not belong to a hybrid-swarmS. asperum × S. officinale with 2 n=48.
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    Plant systematics and evolution 141 (1983), S. 177-185 
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    Keywords: Angiosperms ; Iridaceae ; Iris vartani ; Bulb structure ; vegetative reproduction ; bulblet germination
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    Notes: Abstract Structure and behaviour of the underground organs ofIris vartani were investigated in connection with the vegetative reproduction of this species. Under favourable conditions, well developed mature plants give rise to two replacement bulbs and to several propagation bulblets each year. In spite of this considerable vegetative propagation, no crowding occurs at the natural habitat. Controlled experiments show that as soon as a small group is formed, each bulb produces only one replacement bulb and not two as before. Moreover, the bulblets do not sprout while they are attached to the respective mother bulbs, but rather die and disintegrate, remaining as empty peels. In contrast, detached bulblets, sown individually in separate pots, germinate readily. Consequently, the aggregates ofI. vartani do not increase to any great extent and thus ensure their survival “in situ” for many years.
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    Plant systematics and evolution 141 (1983), S. 243-255 
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    Keywords: Angiosperms ; Caryophyllaceae ; Petrorhagia prolifera ; P. velutina ; P. glumacea ; P. nanteuilii ; Chromosome numbers ; karyotype ; meiotic behaviour ; polyploidy
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    Notes: Abstract Cytogenetic investigations have been made in the fourPetrorhagia species and hybrids of the sectionKohlrauschia. The three diploid species show close similarities in chromosome number, size and morphology, with the exception ofP. velutina, where one pair of metacentric chromosomes is represented by a pair of telocentrics. Meiotic studies in hybrids indicate close genomic homology between the diploid species and also between the two floral forms ofP. prolifera. The tetraploidP. nanteuilii behaves as an allotetraploid forming only bivalents at meiosis and results suggest thatP. velutina andP. prolifera are the diploid progenitors of this species. Since meiosis in diploid and triploid hybrids results in extensive intergenomic pairing it is concluded that the natural tetraploid has a bivalent promoting mechanism that prevents pairing between the genomes of its diploid progenitors.
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    Plant systematics and evolution 141 (1983), S. 231-241 
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    Keywords: Angiosperms ; Liliiflorae ; Liliaceae ; Aphyllanthaceae ; Xanthorrhoeaceae ; Aphyllanthes monspeliensis ; Morphogenesis ; anatomy ; vestigial cambium ; secondary thickening ; ultrastructure
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    Notes: Abstract Aphyllanthes monspeliensis is an isolated member of theLiliiflorae endemic to the Mediterranean region. It is herbaceous, but in its rhizome possesses a secondary thickening meristem (STM). The secondary tissues produced are similar to those known in various arborescent Monocotyledons. Ultrastructural features of the STM cells are described. A relictual intrafascicular cambium also occurs in the bundles of the primary system of the rhizome.
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    Plant systematics and evolution 143 (1983), S. 1-16 
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    Keywords: Angiosperms ; Compositae ; Dispersal ; pappus ; growth form: annuals ; biennials ; perennial herbs ; shrubs and trees ; weeds and non-weeds
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    Notes: Abstract The relationship between gross morphological dispersal structures, growth habit, and weediness is reported for a worldwide sample of 5893 Compositae species from 18 regional floras. A significantly smaller percentage of annual species than perennial species has dispersal structures. Among species with dispersal structures, plumed types are more frequent in perennials while adhesive types are more frequent in annuals. Differences in the occurrence and nature of dispersal structures between perennial herbs, shrubs and trees were minor. Weeds are less likely to have well developed morphological dispersal structures than non-weeds. The data support the idea that dispersal in space may be more important for perennial or non-weedy plants than for annual and weedy plants.
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    Plant systematics and evolution 143 (1983), S. 227-232 
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    Keywords: Angiosperms ; Magnoliidae ; Illiciaceae ; Illicium floridanum ; Ballistic dispersal ; seed dispersal ; vegetative propagation ; early angiosperm evolution
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    Notes: Abstract The primary mode of fruit dispersal in the woodyMagnoliidae is animal dispersal.Illicium floridanum and other members of the genus have a well development system of ballistic fruit dehiscence. The ballistic dispersal is effective only over short distances, but may be important in propelling the seeds away from the parent tree. The presence of explosive dehiscence mechanisms in relatively primitive angiosperm genera suggests that a diversity of dispersal mechanisms may have been present in early angiosperms.
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    Plant systematics and evolution 143 (1983), S. 277-283 
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    Keywords: Angiosperms ; Chenopodiaceae ; Beta ; Reproductive biology ; allogamy versus autogamy
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    Notes: Abstract Populations of different taxa withinBeta sect.Beta were compared using a number of floral and reproductive characters. Plants which were known to be self-incompatible (through S-allele incompatibility) were found to be morphologically distinct from those which were self-compatible, with the floral characteristics of the latter promoting their autogamy.
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    Keywords: Angiosperms ; Boraginaceae ; Symphytum ; Phytosterols ; triterpenoids ; chemotaxonomy
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    Notes: Abstract From a comparison of phytosterol and triterpenoid patterns of severalSymphytum officinale cytotypes,S. asperum and their interspecific hybrids,S. ×uplandicum, which were obtained from thin layer chromatography and gaschromatography (also in combination with mass spectrometry), the hybrid character of the latter taxon is clearly shown. The specific value of the triterpenoid isobauerenol as a chemotaxonomical marker within this group is discussed in some detail.
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    Plant systematics and evolution 143 (1983), S. 311-329 
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    Keywords: Angiosperms ; Compositae ; Calendula ; Selfing ; interspecific hybrids ; speciation
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    Notes: Abstract Breeding experiments were carried out inCalendula species. In the annuals, which are selfers, rarely some outcrossing was observed only in the most peripheral flowers. In experimental crosses fruit was produced in all combinations. Fertile F1 and F2 hybrids could be grown from crosses between parents with similar chromosome numbers:C. palaestina ×C. pachysperma and crosses of different morphological forms ofC. arvensis. In crosses of species with different chromosome numbers at least partly fertile F1 hybrids were obtained fromC. tripterocarpa ×C. stellata andC. tripterocarpa ×C. arvensis and crosses of the latter withC. palaestina. Fertile F2 plants were grown from the combination ofC. arvensis ×C. tripterocarpa. Considering this information and previously obtained data, a scheme is proposed for explaining speciation in the genusCalendula.
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    Plant systematics and evolution 144 (1984), S. 59-66 
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    Keywords: Angiosperms ; Araceae ; Hapaline ; Hapale ; Phymatarum ; Bucephalandra ; Flora of Borneo ; East Malaysia ; Sarawak
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    Notes: Abstract On the basis of newly collected and cultivated material full descriptions and illustrations are presented forHapaline appendiculata Ridl. andPhymatarum borneense M. Hotta.
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    Plant systematics and evolution 144 (1984), S. 67-71 
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    Keywords: Angiosperms ; Scrophulariaceae ; Veronica fridericae spec. nova ; V. acrotheca ; V. farinosa ; V. armena ; V. multifida ; V. jacquinii ; V. caucasica ; V. filifolia ; V. oltensis ; Flora of Turkey
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    Notes: Abstract Veronica fridericae spec. nova, described from the type locality in the S.E. Turkish province Van, is related toV. acrotheca andV. farinosa, both endemic to W. Iran. All 8 perennial species with pinnatifid leaves in S.W. Asia are presented in a key.
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    Plant systematics and evolution 144 (1984), S. 149-150 
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    Keywords: Angiosperms ; Campanulaceae ; Campanula schimaniana spec. nova ; Flora of Afghanistan and of Pakistan
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    Notes: Abstract Campanula schimaniana is a new species of sect.Campanula subsect.Rupestres, distributed in the westernmost mountains affected by monsoon.
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    Plant systematics and evolution 144 (1984), S. 151-153 
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    Keywords: Angiosperms ; Compositae ; Asteraceae-Heliantheae ; Calea ; Chromosome numbers ; polyploidy
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    Topics: Biology
    Notes: Abstract Chromosome numbers are reported for six taxa ofCalea, among them tetraploidC. septuplinervia, the only known polyploid inCalea s. str. The base chromosome number ofCalea is interpreted as x = 19.
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    Plant systematics and evolution 144 (1984), S. 155-163 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Leguminosae ; Fabaceae ; Caesalpinioideae ; Mimosoideae ; Faboideae ; Phaseolus coccineus ; Serological systematics ; seed proteins ; phytohaemagglutinin ; vicilin ; phaseolin
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    Topics: Biology
    Notes: Abstract An immunosystematical research was conducted on the seed proteins of 32 species ofFabaceae. By comparing all immunelectrophoretic patterns with the self reaction of a reference system (Phaseolus coccineus) all proteins detected have been identified and their distribution within the family has been analyzed. Half of the proteins identified inP. coccineus gave positive cross reactions with proteins present in all other species. Among this group are “protein I” and “phaseolin”. This result supports the homologization between phaseolin and vicilin. Among the other proteins, three are irregularly distributed throughout the family, and only three are restricted to a few taxa. This last group includes phytohaemagglutinin, which does not present any cross reaction outside the tribePhaseoleae.
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    Plant systematics and evolution 144 (1984), S. 209-220 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticum ; Elytrigia ; wheat ; Evolution ; genome ; karyotype
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract The lengths of the A, B, and D genomes of common wheat,Triticum aestivum, were measured from the karyotype. Relative to the B genome, standardized as length 1.000, the lengths of the A and D genomes were 0.835 and 0.722, respectively. The lengths of the chromosome arms in the A and D genomes were then multiplied by the appropriate constants so that the total lengths of each genome also equalled 1.000. These calculations revealed that homoeologous chromosomes in wheat, with a few exceptions, have similar sizes and arm ratios. The arm lengths of the three homoeologues in each homoeologous group were then averaged. These average chromosomes turned out to be remarkably similar, in size and arm ratio, to their homoeologues in the E genome ofElytrigia elongata. This evidence and data on cross-compatibility and morphological characteristics suggested that the genusTriticum is a result of adaptive radiation from the perennial genusElytrigia, specifically from the complex of species possessing the E genome or one closely related to it.
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    Plant systematics and evolution 144 (1984), S. 307-314 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Geraniaceae ; Erodium ; Monsonia ; Geranium ; Taxonomic revision ; Flora of Egypt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A key is provided for the 14Erodium species of the Egyptian flora. The important differential chracters of leaf, inflorescence, flower, and fruit are discussed and illustrated.
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    Plant systematics and evolution 144 (1984), S. 315-320 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Fabaceae ; Lupinus palaestinus ; L. pilosus ; Breeding system ; insect induced self pollination
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    Notes: Abstract Significant increase of pod production occurs inLupinus palaestinus Boiss. andL. pilosus Murr. following insect visits. The cause of this increase is investigated through (1) examination of the biology of pollination, (2) examination of pod production under various pollination conditions, (3) examination of cross pollination by genetical markers. All data strongly suggest that “Insect Induced Self Pollination” is the main factor in the increase of pod production of these species in nature.
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    Plant systematics and evolution 145 (1984), S. 1-13 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticum aestivum ; Aegilops longissima ; Cytogenetics ; homoeologous relationships ; chromosome substitutions ; translocations
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    Topics: Biology
    Notes: Abstract The relationships of three wheat-Aegilops longissima chromosome addition lines A, C, and D with homoeologous wheat chromosomes were studied in PMC meiosis. Substitutions of alien chromosome A for wheat chromosome 6 B, chromosome C for 1 B and chromosome D for 4 B were obtained. The production of 4 BS/C and 7 BS/D chromosome translocations indicated cytogenetic relationships of C partially to homoeologous wheat chromosomes of group 1 and 4, and D partially to homoeologous wheat chromosomes of group 4 and 7.
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    Plant systematics and evolution 145 (1984), S. 15-28 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Gramineae ; Hordeum spontaneum ; Wild gene pool ; factor analysis ; numerical taxonomy
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    Topics: Biology
    Notes: Abstract Samples from 11 populations of wild barley,Hordeum spontaneum, from Israel, were examined for morphological variation in a “common garden” plot design. Earliness traits had the highest between population variation of all traits studied. No relationship was found between dimensions of leaves and size of seeds.—Using numerical taxonomy methods, four races were found, which correspond to the geographical and environmental range of the species in Israel. It is concluded thatH. spontaneum shows a well developed tendency toward formation of highly adaptive races rather than exhibiting clinical variation.
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  • 82
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    Keywords: Angiosperms ; Annonaceae ; Porcelia goyazensis ; Annona reticulata ; Winteraceae ; Drimys brasiliensis ; Giemsa C-banding ; structural heterozygosity ; karyotype evolution in tropical woody plants
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    Notes: Abstract InPorcelia goyazensis (2n = 18) Giemsa C-banding patterns differ from those ofAnnona reticulata (2n = 14) and reveal structural heterozygosity. The amplitude of karyological variation in theAnnonaceae is greater than expected for a “primitive” woody family. In a comparison with other tropical angiosperm groups, the highly differentiated karyotype ofDrimys brasiliensis (2n = 86) is interpreted as being the end-point of numerous karyological changes.
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    Plant systematics and evolution 145 (1984), S. 55-77 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Acanthaceae ; Bignoniaceae ; Bombacaceae ; Caesalpiniaceae ; Caryocaraceae ; Chrysobalanaceae ; Fabaceae ; Lobeliaceae ; Lythraceae ; Malvaceae ; Marcgraviaceae ; Mimosaceae ; Musaceae ; Onagraceae ; Orchidaceae ; Passifloraceae ; Proteaceae ; Rubiaceae ; Solanaceae ; Tiliaceae ; Zingiberaceae ; Flower nectar ; amino acids ; sugars ; evolutionary significance ; pollination biology
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    Notes: Abstract Individual amino acids and sugars from flower nectar of 32 plant species with different pollination systems were quantified and compared. Data show that there is no correlation between sugar and amino acid concentration. Furthermore there is no correlation between composition and concentration of amino acids and evolutionary advancement, nor any direct relation with pollination systems. However, higher sugar concentrations are often linked with more advanced morphological characters. Nectars from pierced or damaged flowers or nectars contaminated with pollen exhibit modifications and increases in amino acid composition. The presence of proline probably indicates such pollen contamination. Most pollinating animals depend on flower nectar in their energetic requirements, yet innumerable alternative amino acid and protein sources exist. Future research has to consider the relationship between nutritional requirements of pollinating animals and dependence on flower nectars.
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    Plant systematics and evolution 145 (1984), S. 135-153 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Senecio vulgaris ; S. vernalis ; Autotetraploidy ; self-compatibility ; annual weeds
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    Topics: Biology
    Notes: Abstract Senecio vulgaris L. (2n = 40) is suggested to be of autotetraploid origin fromS. vernalis Waldst. & Kit. (2n = 20). This conclusion is based on results obtained from experimental hybridisations and cytological observations, and the consideration of morphological affinities, patterns of geographical distribution and hybrid formation under natural conditions. The morphological differences between the two species are related to a difference in the breeding system. WhilstS. vernalis is self-incompatible,S. vulgaris is self-compatible and strongly self-pollinating. Equally, other self-pollinating taxa traditionally associated withS. vulgaris are shown to have evolved independently from outbreeding relatives. Within a narrow frame of relationship, annual weeds have evolved in different cytotaxonomic circumstances.
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    Plant systematics and evolution 145 (1984), S. 165-168 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Solanaceae ; Datura innoxia ; Haploidy ; diploidy ; triploidy ; tetraploidy ; hexaploidy ; amino acid composition
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    Topics: Biology
    Notes: Abstract In haploid, diploid, triploid, tetraploid and hexaploid cytotypes of the scopalamine-yieldingDatura innoxia, the amino acid profiles were studied. The results suggest that the amino acid composition is influenced quantitatively by increasing ploidy levels, but in an irregular way.
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    Plant systematics and evolution 145 (1984), S. 183-191 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Saxifragales ; Cunoniales ; Brunelliaceae ; Brunellia comocladiifolia ; B. mexicana ; Theales ; Caryocaraceae ; Caryocar brasiliense ; C. microcarpum ; C. villosum ; Karyosystematics ; paleopolyploidy ; tropical woody plants
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    Notes: Abstract Chromosome numbers are polyploid, 2n = 28 inBrunellia comocladiifolia andB. mexicana, and 2n = 46 inCaryocar brasiliense, C. microcarpum andC. villosum. The chromosome are small in both genera, with a length of ca. 1,6-0,4µm. Interphase nuclei correspond to the prochromosomal and the chromocentric type, respectively. This is in conformance with the systematic placement ofBrunelliaceae intoCunoniales, and ofCaryocaraceae intoTheales. Brunellia exhibits affinities to various other orders ofRosidae (andHamamelididae), and is suggested to be primarily apetalous. On a comparative basis, the chromosome numbers found in both families are interpreted as paleopolyploid (4 x and 6 x). This apparently is in correspondence with their rather primitive features, systematic isolation, relatively depauperate status, and evidently great age.
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    Plant systematics and evolution 145 (1984), S. 193-201 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Ranunculaceae ; Aconitum ; Seed morphology ; seed coat SEM microcharacteristics ; taxonomic importance ; adaptative ecological significance
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    Notes: Abstract The seed coat morphology, investigated in taxa representative of the main European groups ofAconitum, are in good agreement with the current taxonomy of the genus. The seed coat microcharacteristics (warty epidermal cells) are very constant. There is a trend for the reduction of longitudinal wings on the edges concomitant with the development of ridges and transverse wings on the faces. Another morphological progression leads from smooth to rugulose and eventually to transverse wing-bearing seed faces. A working hypothesis suggests an ecological adaptative significance to these changes.
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    Plant systematics and evolution 145 (1984), S. 223-226 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Theaceae ; Camellia japonica ; Allozymes ; polyploidy ; origin of cultivars
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    Notes: Abstract Electrophoretic examination of allozymes from 189Camellia japonica cultivars revealed some banding patterns not explainable by codominant diploid genetics. At several loci encoding dimeric enzymes, 5 and 6 banded patterns were observed in 7 cultivars. These patterns are interpreted as resulting from triploidy or aneuploidy, where three variant alleles code for products which are electrophoretically distinguishable and associate to form three homodimers and three heterodimers. The presence of allozyme multiplicity in these clones suggests a sexual rather than a somatic mode of triploid origin.
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    Plant systematics and evolution 145 (1984), S. 243-258 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Lactuceae ; Microseris ; Canalized numbers ; additive polygenes ; evolutionary genetics ; pappus parts ; leaf shape ; isoenzymes
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    Topics: Biology
    Notes: Abstract CrossingMicroseris pygmaea (10 pappus parts) withM. bigelovii (5 pappus parts) results in hybrids with variable pappus part numbers between 5 and 10. Previous work has shown that a system of four additively acting genes determines the average pappus part numbers of these hybrids. In hybrid B87 two genes have a 10-determining and a 5-determining allele each, two others a 5-determining and a null (inactive or missing) allele. Genetic linkage of one of the latter with the enzyme geneEsterase-1 and the leaf shape genespatulate leaves has been demonstrated. Here we demonstrate linkage between one of the two 10-determining genes and the enzyme locusEsterase- Y/B. The genotypes in the pappus part system of many specimens can now be fully determined. This is a major advance for the analysis of the evolution of this additive polygenic system.
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  • 90
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    Keywords: Angiosperms ; Caryophyllaceae ; Silene latifolia ; S. alba ; S. pratensis ; Geographic variation ; racial differentiation ; clines ; morphological variation ; flavone glycosylation genes ; principal components analysis ; cluster analysis ; Flora of Europe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphological and biochemical data were analysed from 30 greenhouse-grown populations of EuropeanSilene latifolia. Six separate character sets (flavones, seed, pollen, capsules, male and female flower morphology) were used in the analyses. There was broad-scale congruence between trends of geographic variation in most character sets, with the populations being assigned to western (or southern and western) and eastern clusters. The eastern and western clusters abut along a transition zone that runs roughly from Belgium to the northern Balkans; this zone represents a region of relatively rapid change and contains populations intermediate between the eastern and western clusters. Variation in flower morphology was weak and discordant with variation in the other character sets. The origin and maintenance of the variation pattern is discussed in terms of migrational history and “hybrid zones”.
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    Plant systematics and evolution 145 (1984), S. 291-309 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Cruciferae ; Brassicaceae ; Biscutella variegata complex ; B. laevigata complex ; Chromosome numbers ; variation ; aneuploidy and polyploidy ; chromosomal imbalance ; protogyny ; inbreeders and outbreeders
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    Notes: Abstract Chromosome counts were determined for 46 populations ofBiscutella representing 28 taxa. The genus was found to contain diploid taxa with 2n = 12, 16 and 18, tetraploid taxa with 2n = 36 and hexaploid taxa having 2n = 54.B. laevigata L. s. l. consists of diploid and tetraploid populations which are poorly differentiated morphologically. TetraploidB. laevigata s. l. and hexaploidB. variegata Boiss. & Reuter (s. l.) are characterized by chromosomal instability. The variation in chromosome numbers and the occurrence of polyploidy is discussed in relation to the taxonomy of the genus. An investigation of the breeding system showed that most of the annual species were self-compatible and partly inbreeding and most of the perennial species self-incompatible and, therefore, outbreeding, while one annual species,B. cichoriifolia Loisel., showed both systems.
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    Plant systematics and evolution 146 (1984), S. 1-11 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Liliaceae ; Hyacinthaceae ; Scilla peruviana ; Gene mapping ; restriction enzymes ; ribosomal RNA genes ; rRNA/DNA hybridization ; polyploidy
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    Notes: Abstract Scilla peruviana biotypes have different chromosome numbers due to changes in the nucleolar chromosomes and polyploidy. We have examined two diploid (2n = 15 and 2n = 16) and two tetraploid biotypes (2n = 28 and 2n = 32). From the results of rRNA/DNA filter hybridizations it appears that rDNA percentages of the diploid biotypes are, approximately, 2.2-fold higher than those of the tetraploid biotypes. To examine the rRNA gene structure we have utilizedSouthern blot hybridization after DNA digestions with three restriction enzymes: Eco RI, Hind III and Bam HI. From the band analysis of both single and double digestions it has been possible to reveal the presence, in the diploid biotypes, of three gene types, heterogeneous both for length and for nucleotide sequences in the external spacer. The three rRNA genes are 12 600, 12 700, and 12 800 base pairs long and they have a different position of the Hind III sites in the external spacer. On the other hand, a single gene type of 12 600 base pairs, identical to the first type of the diploid biotypes, surprisingly exists in the tetraploid biotypes. Considerations on the rRNA gene regulation and evolution are made.
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    Plant systematics and evolution 146 (1984), S. 13-30 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rutaceae ; Chromosome number ; karyotaxonomy ; base number ; karyotype variation ; literature critique
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    Topics: Biology
    Notes: Abstract Detailed chromosome counts have been made in 61 species, belonging to 33 genera ofRutaceae. 30 of these species are reported here for the first time. For 18 species at least one previous publication gives a chromosome number differing from that reported here. Such discrepancies are, in most cases, due to errors in counting or identification of the material. By critically reviewing the literature on each particular case, it appears possible to eliminate most of the false data. On the basis of the present results, the base number x = 10 is proposed for the genusRuta.
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    Plant systematics and evolution 146 (1984), S. 47-55 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Annonaceae ; Chromosomes ; polyploidy ; karyosystematics ; cytotaxonomy
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    Notes: Abstract Information and illustrations on somatic chromosomes, structure of interphase nuclei and hair development are presented for 9 genera and 14 species ofAnnonaceae. A few suggestions tend to improve the unsatisfactory current classification. The spectrum of polyploids found in the family ranges from neo- to meso- and paleopolyploids.
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    Plant systematics and evolution 146 (1984), S. 87-104 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Iridaceae ; Sisyrinchieae ; Tigridieae ; Trimezieae ; Basic numbers ; chromosome size ; karyotypes ; polyploidy ; karyotypic asymmetry ; bimodality ; DNA increase ; chromosome evolution
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    Notes: Abstract Although the South AmericanIridaceae are cytologically diverse, three tribes are distinguishable on the basis of karyotype morphology. TheSisyrinchieae andTrimezieae have variable basic numbers, ploidy levels and chromosome sizes, while theTigridieae are characterized by a relatively uniform basic number and bimodal karyotype. Changes in chromosome size within genera may suggest fluctuations in their DNA amount with latitude and altitude, particularly inSisyrinchium. The results are considered in terms of opportunities for more detailed research.
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    Plant systematics and evolution 146 (1984), S. 117-121 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Embryology ; megagametophyte ; antipodals proliferation
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    Topics: Biology
    Notes: Abstract The structure of the grasses megagametophyte is considered to be characteristic enough as to deserve a particular place in the megagametophyte typology. Furthermore, it is compared with those of other Monocotyledonous families to point out embryological affinities.
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    Plant systematics and evolution 146 (1984), S. 105-116 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Myrtales ; Oliniaceae ; Olinia ; Embryology ; reproductive anatomy ; systematics
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    Topics: Biology
    Notes: Abstract Two of the five species ofOliniaceae (Olinia emarginata andO. ventosa), a monotypic and problematic family of theMyrtales, were investigated embryologically.Oliniaceae clearly agree with otherMyrtales in their basic embryological characteristics, and are characterized further by having an ephemeral endothecium, a campylotropous ovule, and a thick, three-five-layered, outer integument. A combination of these three characteristic features is unknown elsewhere inMyrtales, so that embryological features do not support a close relationship with any other member of the order. Shared distinctive anther characteristics (i.e. ephemeral endothecium) suggest thatOliniaceae are derived from the common ancestor ofCrypteroniaceae s. str.,Rhynchocalycaceae, Alzateaceae, andPenaeaceae.
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    Plant systematics and evolution 146 (1984), S. 141-143 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Lamiaceae ; Salvia tuxtlensis spec. nova ; Flora of Mexico
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    Notes: Abstract Salvia tuxtlensis, sp. n., is immediately recognized by its semipersistent bracts and yellow flowers. Its affinities are not clear. It is endemic to the Soteapan area in the Sierra de Los Tuxtlas, in Veracruz, Mexico.
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    Plant systematics and evolution 146 (1984), S. 123-139 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Boraginaceae ; Rochelia ; Rochelia disperma ; Gynoecial development ; fruit development ; pseudomonomery ; systematics ; evolutionary trends
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    Topics: Biology
    Notes: Abstract The ontogeny of the gynoecium ofRochelia disperma has been investigated by LM and SEM. From the floral apex only one carpel primordium arises abaxially and eventually shifts into a subterminal position. Neither an initial stadium of a second carpel nor an adaxial vascular strand in gynobase and style could be observed. InR. stylaris two vascular strands run through the style and two undifferentiated lobes in adaxial position may be regarded as rudimentary mericarps. Only from comparison with related taxa the conclusion can be drawn thatRochelia is really pseudomonomerous, more so inR. disperma than inR. stylaris. The primary gynoecial bulge splits up into three parts inR. disperma: style/stigma, nutlets with gynobase, and disc. While the mericarps originate in mostBoraginoideae from the symplicate region and the ascidiate one is restricted to the very basal parts, inR. disperma the ascidiate part extends and forms the nutlets. The hood-shaped mouth of the carpel (the plicate zone) is closed to a triangular slit in lateral position, the stigma. The nutlets are triangular with broad base and do not surround the adaxial part of the gynobase inR. disperma, R. persica, R. bungei, R. stylaris, andR. macrocalyx. In contrast,R. peduncularis, R. cardiosepala, andR. cancellata have nutlets clasping the gynobase; they may be more closely related than was assumed up to now. The glochids ofRochelia are fascicled unicellular hairs (with different shapes) and not emergences as in theCynoglosseae. There is an evolutionary trend towards fruit formation with only one mericarp, especially inR. disperma.
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    Plant systematics and evolution 146 (1984), S. 171-179 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rosaceae ; Fragaria ; Restitution ; microsporogenesis ; polyploidy ; evolution
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    Topics: Biology
    Notes: Abstract Double restitution has been observed cytologically for the first time in microsporogenesis of a F1 hybridFragaria virginiana ×F. chiloensis ♂. Restitution is probably due to irregularities affecting the spindle mechanism. Single or double restitution may depend upon the duration of the effect or upon the stage of meiosis affected. The occurrence of triades is indicative of a possible intracellular diversity. Although the reason which may cause restitution remains to be ascertained, maternal inheritance indicates an extrachromosomal cause.
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