Abstract
The ultrastructure of the canal cells and the canal filling substance ofCitrus limon have been studied. At maturity the canal cells are very rich in cytoplasm. Their inner tangential walls lining the canal are much thickened and formed by two layers: the outer corresponds to the original wall, the inner is formed by subsequent deposition of abundant materials of different origin. This thickening occurs at the same time as the filling of the stylar canal. Both events are paralleled by considerable dictyosomic activity, the formation of a large amount of rough endoplasmic reticulum, and the incorporation of small cytoplasmic masses into the cell wall, due to plasmalemma evaginations. — The material in the stylar canal has a heterogeneous ultrastructure aspect and consists of polysaccharides, proteins and lipids; it presumably provides nutrients for the growing pollen tubes.
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References
Barka, T., Anderson, P., 1965: Histochemistry: Theory, Practice and Bibliography. — New York: Harper and Rose, Hoeber Medical Division.
Bell, J., Hicks, G., 1976: Transmitting tissue in the pistil of tobacco: light and electron microscopic observations. — Planta131, 187–200.
Cresti, M., van Went, J. L., Pacini, E., Willemse, M. T. M., 1976: Ultrastructure of transmitting tissue ofLycopersicon peruvianum style: development and histochemistry. — Planta132, 305–312.
, Ciampolini, F., Sansavini, S., 1980a: Ultrastructural and histochemical features of pistil ofMalus communis: the stylar transmitting tissue. — Scientia Horticulturae12, 327–337.
,, Tiezzi, A., 1980b: Ultrastructural investigations onCitrus limon (L.)Burm. stigma. — (Seventh European Congress on Electron Microscopy, The Hague, August 24th 29th.) — Electron Microscopy2, 252–253.
Dashek, W. V., Thomas, M. R., Rosen, W. G., 1971: Secretory cells of Lilypistils. II. Electron microscope cytochemistry of canal cells. — Amer. J. Bot.58, 909–920.
Feder, N., O'Brien, T. P., 1968: Plant microtechnique: some principles and new methods. — Amer. J. Bot.55, 123–142.
Gori, P., 1977: Ponceau 2 R staining on semi-thin sections of tissues fixed in glutaraldehyde-osmium tetroxide and embedded in epoxy resins. — J. Microsc.,110, 103–105.
Hanf, M., 1935: Vergleichende und entwicklungsgeschichtliche Untersuchungen über Morphologie und Anatomie der Griffel und Griffeläste. — Beih. Bot. Centralbl.54, 99–141.
Heslop-Harrison, J., 1978a: Recognition and response in the pollen-stigma interaction. — InCurtis, A. S. G., (Ed.): Cell-Cell Recognition, 121–138. — Cambridge: Cambridge University Press.
, 1978b: Genetics and physiology of angiosperm incompatibility systems. — Proc. R. Soc. London, ser. B,202, 73–92.
Jensen, W. A., 1962: Botanical Histochemistry. — San Francisco: Freeman.
, Fischer, D. B., 1969: Cotton embryogenesis: the tissue of the stigma and style and their relation to the pollen tube. — Planta84, 97–121.
Kroh, M., 1973: Nature of the intercellular substance of the stylar transmitting tissue. — InLoewus, F., (Ed.): Biogenesis of Plant Cell Wall Polysaccharides, 195–205. — New York: Academic Press.
Kroh, M., van Bakel, C. H. J., 1973: Incorporation of label into the intercellular substance of stylar transmitting tissue formPetunia pistils labelled with tritiated myo-inositol. An electron-microscopic autoradiographic study. — Acta Bot. Neerl.22, 106–111.
Loewus, F., Labarca, C., 1973: Pistil secretion product and pollen tube wall formation. — InLoewus, F., (Ed.): Biogenesis of Plant Cell Wall Polysaccahrides, 175–193. — New York: Academic Press.
Pearse, A. G. E., 1961: Histochemistry, Theoretical and Applied. 2nd ed. — Boston: Little Brown.
Rosen, W. G., Thomas, H. R., 1970: Secretory cells of Lily pistils. I. Fine structure and function. — Amer. J. Bot.57, 1108–1114.
, 1971: Pistil-pollen interactions inLilium. — InHeslop-Harrison, J., (Ed.): Pollen Development and Physiology, 239–254. — London: Butterworths.
Sassen, M. M. A., 1974: The stylar transmitting tissue. — Acta Bot. Neerl.23, 99–108.
Sedgley, M., Buttrose, M. S., 1978: Structure of the stigma and style of the avocado. — Austr. J. Bot.26, 667–682.
Schnepf, E., 1974: Gland cells. — InRobards, A. W., (Ed.): Dynamic Aspects of Plant Ultrastructure. — London: McGraw Hill.
Spurr, A. R., 1969: A low viscosity epoxy resin embedding medium for electron microscopy. — J. Ultrastruct. Res.26, 31–43.
Thiéry, J. P., 1967: Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. — J. Microscopie6, 987–1018.
Vasil, I. K., 1974: The histology and physiology of pollen germination and pollen tube growth in the stigma and in the style. — InLinskens, H. F., (Ed.): Fertilization in Higher Plants, 105–118. — Amsterdam: North Holland.
, Johri, M. M., 1964: The style, stigma and pollen tube. — Phytomorphology14, 352–369.
Vasil'ev, A. E., 1970: Ultrastructure of stigmatoid cells inLilium. — Fiziol. Rast.17, 1240–1248; translated in Soviet Plant Physiol.17, 1035–1044.
Welk, Sr. M., Millington, W. F., Rosen, W. G., 1965: Chemotropic activity and the pathway of the pollen tube in Lily. — Amer. J. Bot.52, 774–781.
Wilms, H. J., 1980: Ultrastructure of the stigma and style of spinach in relation to pollen germination and pollen tube growth. — Acta. Bot. Neerl.29, 33–47.
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Research performed under CNR program “Biology of Reproduction”.
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Ciampolini, F., Cresti, M., Sarfatti, G. et al. Ultrastructure of the stylar canal cells ofCitrus limon (Rutaceae). Pl Syst Evol 138, 263–274 (1981). https://doi.org/10.1007/BF00985190
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DOI: https://doi.org/10.1007/BF00985190