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  • 1
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    Fluorodensitometric determination of potato glycoalkaloids on thin-layer chromatograms (1981)
    Elsevier
    Details
    Publication Date: 1981-05-01
    Print ISSN: 0021-9673
    Electronic ISSN: 1873-3778
    Topics: Chemistry and Pharmacology
    Published by Elsevier
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Boekbesprekingen (1979)
    Springer
    Pharmacy world & science 1 (1979), S. 833-836 
    Details
    ISSN: 1573-739X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02293351
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Orthosiphon stamineus, een geneeskruid met een diuretische werking (1979)
    Springer
    Pharmacy world & science 1 (1979), S. 965-970 
    Details
    ISSN: 1573-739X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Samenvatting In dit artikel wordt een overzicht gegeven van het tot dusver bekende fytochemisch en farmacologisch onderzoek naar de werkzame bestanddelen van de bladeren vanOrthosiphon stamineus Benth., dat als geneeskruid wordt toegepast bij nier- en blaasziekten en als diureticum. Hoewel een hoog kaliumgehalte en de aanwezigheid van inositol in het kruid wel verantwoordelijk worden gesteld voor de werking, is er nog geen duidelijke relatie tussen deze bestanddelen en de farmacologische effecten. Mogelijk zouden ook andere stoffen een rol kunnen spelen, zoals nog niet volledig geÏdentificeerde saponinen, terwijl flavonoÏden en hun omzettingsprodukten verantwoordelijk zouden kunnen zijn voor de bacteriostatische werking van Orthosiphon. In ons laboratorium is een onderzoek begonnen teneinde hierover opheldering te verschaffen.
    Notes: Abstract In this paper a survey is given of the phytochemical and pharmacological research, known until now, into the active principles of the leaf drugOrthosiphon stamineus Benth., which is used therapeutically for diseases of the kidneys and the bladder and as a diuretic. Although a high potassium content and the presence of inositol have been claimed for the activity no clear relationship exists between these constituents and the pharmacological effects. Other constituents as for instance not fully identified saponins may possibly play a role and flavonoids and their metabolites would be responsible for the bacteriostatic activity of Orthosiphon. An investigation is in progress in our laboratory to elucidate these problems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02293376
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  • 4
    Electronic Resource
    Electronic Resource
    The essential oil of Valeriana officinalis L. s.l. (1979)
    Springer
    Pharmacy world & science 1 (1979), S. 443-449 
    Details
    ISSN: 1573-739X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The pharmacological action of Valerian is mainly ascribed to the so-called valepotriates, which were first shown to be present in Valerian ByThies (1966). An investigation into the composition of the essential oil of subterranean parts of a commercial Valerian strain has been carried out in order to obtain a clearer insight into its possible contribution to the pharmacological action of this plant. During this investigation, mainly performed by means ofGc-Ms, 69 compounds could be identified of which 45 had not been reported to be present in the essential oil of European Valerian. Three types of essential oil could be distinguished in a comparative investigation of a number of samples of root material from individual plants, namely: Type A: 2.4–4.9% elemol, 6.2–8.7% valeranone and 13.4–15.9% valerenal; no ‘M = 238’ and ‘M = 280’. Type B: 9.8–11.7% elemol, 10.3–12.0% valerenal; no valeranone, ‘M = 238’ and ‘M = 280’. Type C: 1.9–2.8% elemol, 16.2–18.1% valeranone, 9.3–10.3% ‘M = 238’, 3.3–3.9% valerenal and 3.3–3.8% ‘M = 280’. ‘M = 238’ and ‘M = 280’ are presumably a kessyl alcohol and its corresponding acetate respectively. From the results obtained the conclusion can be drawn that, if the essential oil of Valerian plays a role in the pharmacological action of the crude drug, it depends upon the composition of the essential oil. An extended investigation has to be carried out in order to define the original chemotypes ofValeriana officinalis L. s.l.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02293248
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and analytical aspects of Valeriana compounds (1979)
    Springer
    Pharmacy world & science 1 (1979), S. 956-964 
    Details
    ISSN: 1573-739X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Valepotriates are considered as one of the main groups of compounds responsible for the sedative activity of Valeriana preparations. An extraction method for valepotriates is given and the isolation of (iso) valtrate, didrovaltrate andIvhd-valtrate by means of column chromatography on silicagel was shown to be a fast method without giving much decomposition. Toluene-ethyl acetate-methyl ethyl ketone (80+15+5) as a mobile phase gives an excellent indication of the presence of the various valepotriates in Valeriana preparations. For the quantitative determination of valepotriates a direct spectrophotometric scanning onTlc plates was compared with aHplc
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02293375
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  • 6
    Electronic Resource
    Electronic Resource
    Production of L-DOPA by cell suspension cultures of Mucuna pruriens (1985)
    Springer
    Plant cell, tissue and organ culture 3 (1985), S. 61-73 
    Details
    ISSN: 1573-5044
    Keywords: plant cell suspension cultures ; callus cultures ; L-DOPA ; catecholamines ; Mucuna pruriens ; Fabaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methods for induction and maintenance of callus as well as cell suspension cultures of Mucuna pruriens are described. The presence of 3- (3, 4-dihy droxyphenyl 1)-L-alanine (L-DOPA) in these cell suspension cultures was demonstrated by means of TLC and HPLC, the latter being equipped with an ultra-violet or a more specific electrochemical detector. Further methods employed were GC, also in combination with mass spectrometry, and thermal desorption chemical ionization mass spectrometry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041657
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  • 7
    Electronic Resource
    Electronic Resource
    Production of L-DOPA by cell suspension cultures of Mucuna pruriens (1985)
    Springer
    Plant cell, tissue and organ culture 3 (1985), S. 75-82 
    Details
    ISSN: 1573-5044
    Keywords: plant cell suspension cultures ; L-DOPA ; catecholamines ; Mucuna pruriens ; Fabaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The endogenous synthesis of 3-(3,4-dihydroxyphenyl)-L-alanine (L-DOPA) by cell suspension cultures of Mucuna pruriens was found to be influenced by several environmental parameters. The nature of the nitrogen source as well as the concentration of nitrogen containing salts, sucrose and phosphate in the culture medium were found to affect the biosynthesis of L-DOPA. Addition of 2, 4-dichlorophenoxyacetic acid to the medium suppressed L-DOPA production; continuous illumination of the cultures had a strong beneficial effect on L-DOPA production. L-DOPA was accumulated intracellularly by the cell suspension cultures. These observations further demonstrate that for certain products of plant cell suspensions product synthesis can be manipulated by a proper selection of specified nutrients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041658
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  • 8
    Electronic Resource
    Electronic Resource
    Regeneration of plants from tissue- and cell suspension cultures of Symphytum officinale L. and effect of in vitro culture on pyrrolizidine alkaloid production (1983)
    Springer
    Plant cell, tissue and organ culture 2 (1983), S. 227-238 
    Details
    ISSN: 1573-5044
    Keywords: callus cultures ; cell suspensions ; regeneration ; pyrrolizidine alkaloids ; polyamines ; Symphytum ; Boraginaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Primary calluses were induced from various organs of Symphytum officinale L. (comfrey) plants on solid MS and B5 medium supplemented with plant growth regulators. The callus was further subcultured on B5 medium. Cell suspension cultures were derived from B5 grown calluses by transfer to liquid B5 medium. Calluses as well as cell suspension cultures could be induced to regenerate whole plants on solid MS medium. Plants regenerated from short term cultures were identical with plants from which cultures were initiated in morphology and chromosome number. Production of pyrrolizidine alkaloids ceased on prolonged subculturing of suspensions although polyamines, which might act as precursors, were still detectable. However, regenerated plants produced the original alkaloids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033561
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  • 9
    Electronic Resource
    Electronic Resource
    The occurrence of valeranone and crypto-fauronol in the essential oil of Valeriana officinalis L. s.l. collected in the northern part of The Netherlands (1981)
    Springer
    Pharmacy world & science 3 (1981), S. 1316-1320 
    Details
    ISSN: 1573-739X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In this paper the results are presented of an investigation of the essential oils of severalValeriana officinalis L. s.l. populations collected from 29 locations in the northern part of The Netherlands. UsingGc-ms, two chemovars could be distinguished based on the presence or absence of valeranone. Investigation in more detail by means of (TAS)Tlc showed that all plants from six locations contained valeranone, whereas the plants from another six locations were not homogeneous with respect to the presence or absence of this constituent for which pharmacological activity has been reported. The presence of crypto-fauronol could be shown in the valeranone containing plants harvested from only two locations. No direct relation could be shown between plant morphology, composition and pH of the soil and the phenomenon mentioned above. Crossing experiments have to be carried out in order to elucidate how this presence or absence of valeranone is genetically linked. No differences could be shown in the pattern of the valepotriates which are supposed to be the most important pharmacologically active substances of Valerian. The results obtained from the present and earlier investigations make it clear that a qualitative and quantitative investigation of the essential oil of Valerian remains necessary because the differences in the composition of the essential oil concern constituents which may contribute to the sedative action of Valerian and its preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02193381
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  • 10
    Electronic Resource
    Electronic Resource
    Separation of valtrate and isovaltrate by means of preparative liquid chromatography (1982)
    Springer
    Pharmacy world & science 4 (1982), S. 21-24 
    Details
    ISSN: 1573-739X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In this study valtrate and isovaltrate, two isomeric valepotriates present in variousValeriana species, were separated on a preparative scale with a Waters preparative Liquid Chromatography/500 a system. A rapid separation was achieved with the solvent methylene chloriden-propanol-acetone (99 + 0.5 + 0.5). Extensive use of the recycle mode of the instrument resulted in a considerable reduction of the amount of solvent used, without affecting the purity and the recovery (〉 90%) of the separated components. With the method presented in this communication 1 gram of a mixture of valtrate and isovaltrate was separated in 20 minutes using 2.1 liters of solvent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02112353
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