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  • 1
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 61 (1982), S. 327-331 
    ISSN: 1432-2242
    Keywords: Petunia parodii ; Microspore-embryogenesis ; Nuclear fusion ; Triploidy ; Horticulture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A total of 61 microspore-derived plants of Petunia parodii were grown to maturity revealing a predominent population of triploids, 80.3%. Cytological investigations, together with the evidence from microfluorimetry, suggest that the origin of these triploids was due to the fusion of interphase nuclei in two different pathways. In the majority of embryogenic microspores, a vegetative nucleus of 1C DNA content fused with an endo-reduplicated 2C DNA generative nucleus at the binucleate stage and produced true triploid embryoids and plantlets (A pathway). Where this fusion failed, both the vegetative and the generative nuclei divided separately and in the multinucleate microspore two or more daughter nuclei fused to form a mixoploid embryoid. Such mixoploid embryoids produced a mixed population of plants with various ploidy levels as well as ploidy polymorphism within an individual. Since the triploids are morphologically superior with a faster growth rate than their diploids and related tetraploids, a predominent population of triploid plants was obtained from such mixoploid embryoids (B pathway). By low temperature treatment of the anther-donor buds, the embryogenic response of microspores was enhanced up to 5-fold.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 2 (1983), S. 255-256 
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Haploid, diploid and triploid plants in Petunia violacea have been produced by culturing anthers of early binucleate pollen on kinetin supplemented basal medium. Cytological studies reveal that non-haploid embryoids developed due to nuclear fusion at early stages of pollen embryogenesis. The significance of plants of various ploidy levels in this commonly cultivated species of Petunia is discussed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 59 (1981), S. 221-223 
    ISSN: 1432-2242
    Keywords: Chochlearia pyrenaica ; Newly induced autopolyploids ; Established natural autopolyploids ; B chromosomes ; Multivalent suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In diploid Cochlearia pyrenaica, its established natural autotetraploid C. officinalis, and their newly induced autotetraploid and auto-octoploid derivatives, B chromosomes change the normal pattern of chromosome association by suppressing homologous pairing. Frequency of bivalents increases at the expense of multivalents from lower to higher numbers of B chromosomes. The reduction of multivalents due to the direct influence of the B chromosomes, independent of pollen mother cell chiasma frequency, is suggested as being related to the mechanism that prevents A/B chromosome pairing.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1436-6215
    Keywords: enzyme activity ; mitochondria ; mustard oil ; carnitin ; fish oil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary The effects of short-term feeding of a 20 % mustard oil (containing 47 % erucic acid) diet with 0.1 % carnitine and/or fish oil on the activity of enzymes of lipid metabolism in mitochondrial and post-mitochondrial fractions of heart, liver, and skeletal muscles of rats were determined to study the reversal of erucic acidinduced lipidosis. Histopathological changes were also studied. The inclusion of fish oil or fish oil plus carnitine in a mustard oil diet, especially the latter, eliminated the histopathologically detectable lipidosis in heart and skeletal muscles. The reason for this appears to be the increased activity of lipase and phospholipase in tissue homogenates and of cholesterol ester hydrolase in the post-mitochondrial fraction; and also an increase in mitochondrial succinate dehydrogenase which may increase the utilization of the liberated fatty acids. The results suggest that the erucic acid-induced lipidosis is prevented or eliminated by increased lipolysis and oxidation of fatty acids when such a diet is supplemented with carnitine and/or fish oil.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 126 (1994), S. 147-155 
    ISSN: 1573-0832
    Keywords: Cryptococcus neoformans ; Goats ; Mastitis ; Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Unilateral intramammary inoculation of 10 goats withCryptococcus neoformans (2×106 yeast cells) resulted in the development of mastitis, with gross and microscopic lesions being restricted to the infected udder halves only and there was no dissemination of infection to the opposite uninfected udder halves as well as to other organs of the body. The experiment was continued for 40 days, with 2 animals each from the infected and control groups being killed on 5th, 10th, 20th, 30th and 40th day postinoculation (DPI). Initial enlargement of the infected udder halves was followed by marked decrease in size leading to very small, firm and nodular udder halves. After infection, there was also sharp fall in the milk yield. Cryptococcal organisms were demonstrated in the mastitic milk and udder impression smears with special stains.C. neoformans was reisolated from the milk of only infected udder halves up to 25th DPI. Microsopically, there was initially acute diffuse purulent mastitis which later on became chronic, characterised by marked infiltration of lymphocytes, macrophages, extensive fibrosis and development of multiple granulomas. The cryptococcal organisms could be demonstrated in the udder sections only up to 30th DPI. It is concluded that intramammary inoculation ofCryptococcus neoformans in goats leads to severe mastitis with sharp fall in milk yield.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 121 (1993), S. 77-81 
    ISSN: 1573-0832
    Keywords: Aspergillosis ; Goats ; Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intratracheal inoculation of goats withAspergillus fumigatus spores resulted in the development of characteristic gross and microscopic lesions. The lesions were restricted to lungs and there was no dissemination of infection to other tissues of the body except liver in one goat 16 days after infection. The experiment was continued for 37 days. Gross changes in lungs were observed up to the 24th day post-infection. The lesions, in general, included congestion and oedema in the first 6 days followed by the development of varying greyish-white nodules in the lungs. Microscopic changes consisted of granulomatous reaction with well developed granulomas in lungs. Hyphae and conidiophores with fruiting bodies ofAspergillus fumigatus could be demonstrated in sections up to 24 days of infection. Reisolation of the fungus consistently was achieved up to 24 days. It is concluded that intratracheal inoculation ofAspergillus fumigatus spores in goats leads to pulmonary aspergillosis up to 24 days.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 126 (1994), S. 9-14 
    ISSN: 1573-0832
    Keywords: Aspergillosis ; Goats ; Pathology ; Udder
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intramammary inoculation of goats withAspergillus fumigatus spores resulted in the development of mastitis with characteristic gross and microscopic lesions. The mastitis and the lesions were restricted to the infected udder halves only and there was no dissemination of infection to other tissues of the body. The experiment was continued for 45 days. Gross changes in the infected udder were observed up to the 45th day post-infection. The lesions, in general, included variable sized abscesses in the first 15 days followed by development of varying sized greyish-white nodules in the infected udders. Microscopic changes consisted of granulomatous reaction with well developed granulomas in the infected udders. Hyphae and spores ofAspergillus fumigatus could be demonstrated in sections of the infected udders up to 45 days after infection. Reisolation of the fungus consistently was achieved up to 45 days. It is concluded that intramammary inoculation ofAspergillus fumigatus spores in goats leads to chronic granulomatous mastitis.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 138 (1981), S. 23-27 
    ISSN: 1615-6110
    Keywords: Brassicaceae (Cruciferae) ; Cochlearia ; Diploidy ; induced autotetraploidy ; natural autotetraploidy ; induced autooctoploidy ; chromosomes ; nucleic acids ; protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of chromosome doubling on macromolecular composition, i.e. DNA, RNA and protein, were examined in diploidCochlearia pyrenaica DC., its established natural autotetraploidC. officinalis L., and their newly colchicine-induced autotetraploid and autooctoploid derivatives. DNA, RNA and protein contents increase from lower to higher ploidy levels (2x 〉 4x 〉 8x). The established natural autotetraploids and newly induced autotetraploids also differ, as DNA, RNA and protein have been reduced during the course of establishment. Net synthesis of RNA and protein per unit DNA does not change significantly neither from lower to higher ploidy levels nor between the autotetraploids of newly induced and established natural origins.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 145 (1984), S. 165-168 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Solanaceae ; Datura innoxia ; Haploidy ; diploidy ; triploidy ; tetraploidy ; hexaploidy ; amino acid composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In haploid, diploid, triploid, tetraploid and hexaploid cytotypes of the scopalamine-yieldingDatura innoxia, the amino acid profiles were studied. The results suggest that the amino acid composition is influenced quantitatively by increasing ploidy levels, but in an irregular way.
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