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  • nitrogen  (54)
  • Oryza sativa  (47)
  • Springer  (101)
  • 2005-2009
  • 1995-1999  (101)
  • 1997  (101)
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  • 2005-2009
  • 1995-1999  (101)
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  • 1
    ISSN: 1432-0789
    Keywords: Cyanobacteria ; Soil inoculation N ; transformation ; Inorganic N ; Easily oxidizable N ; Hydrolysable N ; Non-hydrolysable N ; Wetland rice Farmyard manure ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted with wetland rice (Oryza sativa cv. IR-36) in a sandy clay loam soil (Entisol) to study the effect of inoculation with a soil-based mixed culture of four diazotrophic cyanobacteria,Aulosira fertilissima, Nostoc muscorum, N. commune andAnabaena spp., on the N-flux in inorganic NH4 ++NO3 −+ NO2 −), easily oxidizable, hydrolysable and non-hydrolysable forms of N in soil during vegetative growth periods of the crop. Effects on grain and straw yield and N uptake by the crop were estimated. The effects of applying urea N and N as organic sources, viz.Sesbania aculeata, Neem (Azardirachta indica) cake and FYM, each at the rate of 40 kg N ha−1, to the soil were also evaluated. Inoculation significantly increased the release of inorganic N, evidenced by its increased concentrations either in soil or in soil solution. However, such increases rarely exceeded even 4% of total N gained in different froms in the soil system by inoculation during the vegetative growth stages of the rice plant, when the nutritional requirement of the plants is at a maximum. Most of the N2 fixed by cyanobacteria remained in the soil as the hydrolysable form (about 85%) during this period. Inoculation caused an insignificant increase in grain (8%) and straw (11%) yield, which was, however, accompanied by a significant increase in N uptake by the grain (30%) and an increase in total uptake of 15.3 kg N ha 1. Such beneficial effects of inoculation varied in magnitude with the application of organic sources, with farmyard manure (FYM) being the most effective. Application of urea N, on the other hand, markedly reduced such an effect.
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  • 2
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    Biology and fertility of soils 25 (1997), S. 285-289 
    ISSN: 1432-0789
    Keywords: Key words Methane emission ; Wetland soils ; Greenhouse gases ; Inceptisol ; Vertisol ; Rice ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a greenhouse study, methane emissions were measured from two diverse Indian rice-growing soils planted to five rice cultivars under similar water regimes, fertilizer applications and environmental conditions. Significant variations were observed in methane emitted from soils growing different cultivars. Total methane emission varied between 8.04 and 20.92gm–2 from IARI soil (Inceptisol) and between 1.47 and 10.91gm–2 from Raipur soil (Vertisol) planted to rice. In all the cultivars, emissions from IARI soil were higher than from Raipur soil. The first methane flux peak was noticed during the reproductive phase and the second peak coincided with the grain-ripening stage of the rice cultivars.
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  • 3
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    Biology and fertility of soils 24 (1997), S. 70-75 
    ISSN: 1432-0789
    Keywords: Flooded rice soil ; Soil-trapped CH4 and C02 ; δ13C values of CH4 and C02 ; Greenhouse effect ; CO2 flux ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pot experiments were conducted to monitor the changes in compositions and δ13C values of soil-trapped CH4 and C02 in flooded rice soil with and without rice plants or rice straw. Incorporation of rice straw increased the concentration of CH4 and C02 accumulated in soil, and the quantities of emitted CH4 to the atmosphere. Rice plants reduced the concentration of soil-trapped CH4 and CO2, and the decreased portion of CH4 was replaced by N2. A significantly negative correlation was found between soil-trapped CH4 and N2. The presence of rice plants increased the δ13C values of CH4. The δ13C values of CH4 tended to increase toward the end of the growing season and were positively correlated with concentration of soil-trapped CH4. A positive correlation between δ13C values of CH4 and C02, and between the δ13C values of CH4 and its concentration, were observed. The CH4 in the rice stems was 4–14% enriched in13C relative to soil-trapped CH4. In contrast, CO2 in rice plant stems was 1–9% lighter in13C relative to soil-trapped C02. These results are discussed in relation to the precursor pools and pathways of methanogenesis.
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  • 4
    ISSN: 1432-0789
    Keywords: Key words Microbial biomass C ; Water-soluble organic carbon ; Light fraction organic carbon ; Fertilizer ; nitrogen ; 13C nuclear magnetic resonance ; Infrared spectrophotometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil samples taken from four experimental sites that had been cropped to continuous corn for 3–11 years in Ontario and Quebec were analyzed to evaluate changes in quantity and quality of labile soil organic carbon under different nitrogen (N) fertility and tillage treatments. Addition of fertilizer N above soil test recommendations tended to decrease amounts of water-soluble organic carbon (WSOC) and microbial biomass carbon (MBC). The quality of the WSOC was characterized by 13C nuclear magnetic resonance and infrared spectrophotometry and the results indicated that carbohydrates, long-chain aliphatics and proteins were the major components of all extracts. Similar types of C were present in all of the soils, but an influence of management was evident. The quantity of soil MBC was positively related to the quantities of WSOC, carbohydrate C, and organic C, and negatively related to quantities of long-chain aliphatic C in the soil. The quantity of WSOC was positively related to the quantities of protein C, carbohydrate C, and negatively related to the quantity of carboxylic C. The quantity of soil MBC was not only related to quantities of soil WSOC but also to the quality of soil WSOC.
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  • 5
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    Biology and fertility of soils 24 (1997), S. 261-265 
    ISSN: 1432-0789
    Keywords: Key words Methane ; Wetland rice soils ; Oryza sativa ; Methane oxidation ; Acetylene Propylene oxide ; Methanotrophs ; Rhizosphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Acetylene up to 500 μl l–1 did not affect methane formation in anoxic soil up to 12 h, but further incubation for 1 week showed strong inhibition of methanogenesis. To ascertain the extent of the oxidation of methane produced from rice-planted pots, the effect of acetylene on methane emission was studied. Two rice varieties (Toyohatamochi and Yamahikari) were grown in a greenhouse in submerged soil in pots. At about maximum tillering, heading, and grain-forming stages, methane fluxes were measured. Flux measurement was performed for 3 h from 6 pm, then acetylene at 100 μl l–1 was added to some of the pots. At 6 a.m. the following day, methane fluxes were again measured for 3 h. Only at maximum tillering stage of the variety Toyohatamochi was a significant increase (1.4 times) in methane flux caused by acetylene observed, whereas in the other treatments no significant increase in methane fluxes by acetylene could be defected. To ascertain the activity of methane monooxygenase (MMO), propylene oxide (PPO) formation from propylene was measured with excised roots and a basal portion of stems of the rice plants grown on the submerged soil. A level of 0.1–0.2 μmol PPO h–1 plant–1 was recorded. The roots showed the highest PPO formation per gram dry matter, followed by basal stems. Methane oxidation was roughly proportional to PPO formation. Soluble MMO-positive methanotroph populations were measured by plate counts. The number of colony-forming units per gram dry matter was 106–105 in roots, and 104–103 in basal stems. These results indicate the possibility of methane oxidation in association with wetland rice plants.
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  • 6
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    Biology and fertility of soils 26 (1997), S. 31-34 
    ISSN: 1432-0789
    Keywords: Key words Microbial biomass ; Air-drying ; Extractable ; nitrogen ; Extractable phosphorus ; Tropical soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The microbial contribution to extractable N and P after the air-drying of eight Indian dry tropical Ultisols was quantified. Air-drying of the soils decreased microbial biomass C by 25–53% but increased extractable N and P by 14–34% and 24–121%, respectively. This increase in the extractable N and P was accounted for, to some extent, by microbial biomass killed due to air-drying. Microbial biomass contributes 17–36% and 19–82% to the extractable N and P, respectively, possibly due to air-drying of the soils. I conclude that due to contamination of microbial biomass with the available nutrients in air-dried soils, measurements of extractable nutrients should be made on field-moist soils.
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  • 7
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    Nutrient cycling in agroecosystems 48 (1997), S. 241-246 
    ISSN: 1573-0867
    Keywords: barley ; denitrification ; fertilizerefficiency ; mineralization ; nitrogen ; sludge
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Enhanced microbial activity following sewage sludge land application may affect soil N cycling and, therefore, plant available N. We studied the effect of anaerobically treated sewage sludge on N-fertilizer efficiency and on some aspects of the soil N cycling. Field plots (3 m × 9 m) sown with barley (Hordeum vulgare L.) in November were amended with a) sludge (80 mg ha-1) and ammonium nitrate (150 kg N ha-1), b) ammonium nitrate (150 kg ha-1) only, c) or left unamended. Monthly soil samples were taken from 0 to 20-and 20 to 50-cm depths to determine soil inorganic N (NH4 +, NO3-). Denitrification in the upper 20-cm horizon was estimated by measuring N2O+N2 emission from undisturbed soil samples by the acetylene-inhibition technique. Crop yield parameters were analysed before harvesting, and grain production was recorded. With respect to the control, the yield increase for the N-fertilizer treatment was 85% and 45% for the sludge + N-fertilizer treatment. The decrease of N-fertilizer efficiency in sludge amended plots was presumably due to a decrease in spring plant available N. Presumably, microbial immobilisation and denitrification in organic amended treatments were responsible for the decrease in N-fertilizer efficiency.
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  • 8
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    Nutrient cycling in agroecosystems 48 (1997), S. 37-50 
    ISSN: 1573-0867
    Keywords: greenhouse gases ; nitrogen ; NOx ; nitrous oxide ; ozone precursors ; trace gases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Over 60 published papers reporting field measurements of emissions of nitric oxide (NO) from soil are reviewed, and over 100 annual estimates of NO emissions were made for various types of ecosystems, including agricultural fields. These data were stratified by biome and the mean of each stratum was multiplied by an estimate of the biome area. A few strata were identified as clearly having low NO emissions: montane forests, swamps and marshes, tundra, and temperate forests that are not heavily affected by N deposition. The largest emissions were observed in tropical savanna/woodland, chaparral, and cultivated agriculture, but variation in NO emissions within these strata was also large. Although the stratification scheme fails to partition this within-stratum variation, it does clearly identify these biomes as globally important sources of NO and as areas where more research is needed to investigate within-biome variation in NO emissions. It is too early to tell whether differences in NO emissions between temperate and tropical agriculture are significant, but it is clear that agriculture is an important source of NO and that management practices affect NO emissions. The best current estimate of the global soil source of NO is 21 Tg N yr-1. Adsorption of NOx onto plant canopy surfaces may reduce emissions to the atmosphere to as low as 13 Tg N yr-1, although the absorption effect is probably smaller than this. An error term for the global estimate is difficult to determine, but it is at least ±4 and perhaps as large at ±10 Tg N yr-1. Hence, only modest progress has been made in narrowing uncertainties in the estimate of the global soil source of NO, although some published lower estimates appear unlikely. This inventory reconfirms that the soil source of NO is similar in magnitude to fossil fuel emissions of NOx. Further narrowing of the uncertainty of the estimate of global soil NO emissions will require more sophisticated and carefully chosen stratification schemes to address variation within biomes based on soil fertility, soil texture, climate, and management and will require linking this type of inventory and stratification with mechanistic models.
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  • 9
    ISSN: 1572-9680
    Keywords: fibre ; intake ; nitrogen ; palatability ; tannin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aim of this work was to assess whether degradability, gas production or chemical constituents could predict the preference of browses. Forty tropical browse species leaves with a crude protein (CP) content ranging from 79 to 307 g kg-1 DM were used for this study. The neutral detergent fibre (NDF) ranged from 220 to 694 g kg-1 DM, while acid detergent fibre (ADF) ranged from 146 to 523 g kg-1 DM. The NDF-bound nitrogen (NDFN) and ADF-bound nitrogen were particularly high in Calliandra calothyrsus, Acacia polyacantha, Sesbania sesban, Acacia venosa and Acacia hockii. High levels of tannins were observed in Acacia species especially A. dolichocephala, A. hockii, A. microbotrya and A. salicina. High levels were also observed in Flemingia macrophyla and Leucaena pallida. The browse species differed (P 〈 0.05) in DM in sacco degradability coefficients. High potential degradability (PD) and effective degradability (ED) were observed in Sesbania spp, Moringa stenopetala, Indigofera arrecta, Chamaecytisus palmensis and Atriplex spp. The browses differed (P 〈 0.05) in asymptotic gas (Ag) production (ml g-1 OM), but had similar (P 〉 0.05) times of incubation at which half of the asymptotic gas had been formed. Preference and DM intake were positively correlated (P 〈 0.01) to NDFN, but negatively correlated (P 〈 0.05) to NDF and ADF. The PD and Ag were negatively (P 〈 0.001) related to NDF, ADF and lignin. Total phenols (TP) and condensed tannins (CT) were negatively (P 〈 0.05) related to PD, ED and Ag. A positive correlation was observed between CT and NDF-bound condensed tannins (r = 0.55, P 〈 0.001) and, CT and TP (r = 0.40, P 〈 0.01). Prediction equations were poor for DM intake and preference, moderate for gas production and good for potential and effective degradabilities. The phenolic components were more related to dry matter degradation and gas production than to preference and dry matter intake. NDFN and Ag made a positive contribution to both preference and DM intake. It was concluded that chemical constitutes such as N, NDF, NDFN, ADF and lignin are essential to predict the nutritive value of browses.
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  • 10
    ISSN: 1432-203X
    Keywords: Oryza sativa ; rice ; somaclonal variation ; genetic integrity ; RAPD analysis ; DNA markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary RAPD analysis was performed among eight rice somaclonal families known to vary for specific characters and four somaclonal families which were phenotypically normal. The parental cultivar,indica rice cv. FR13A, was found to be homogeneous and homozygous at all but one of the 45 RAPD loci. Polymorphisms were found at 28 of the 45 bands among the somaclonal families, including both loss of parental bands, and the appearance of novel non-parental bands. Segregation data revealed both heterozygous and homozygous mutation events, with recessive mutations more prevalent than dominant. All somaclonal families differed significantly from the parental material, indicating that genomic alterations occurred in all families regardless of phenotype. None of the variant families could be regarded as isogenic lines of FR13A at the DNA level. However, some of the DNA level variation may be in highly repeated sequences with no phenotypic effects. The implications for somaclonal breeding and genetic engineering programs are discussed.
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  • 11
    ISSN: 1432-0983
    Keywords: Key words Intermolecular recombination ; Inverted repeat ; Oryza sativa ; Plastid DNA (PtDNA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have determined the nucleotide sequences around the junction points of oligomeric-deleted ptDNAs possessing a head-to-head or tail-to-tail configuration from long-term cultured cell lines and albino plants. It was shown that DNA rearrangement occurred by direct fusion of deleted ptDNAs in an inverted orientation, which was linked by an asymmetrical sequence of 254–698 bp derived from either of the ptDNAs joined. It is notable that inverted repeats of 7–14 bp flank the asymmetrical sequences at each of the junction points. These features of the DNA sequence around the junction points are commonly observed in oligomeric ptDNA with a large-scale deletion regardless of the cell lines employed. It is suggested that the short inverted repeats are involved in the intermolecular recombination of ptDNA.
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  • 12
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    Environmental and resource economics 10 (1997), S. 341-362 
    ISSN: 1573-1502
    Keywords: Baltic Sea ; eutrophication ; nitrogen ; phosphorus ; cost effective
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Economics
    Notes: Abstract Due to eutrophication caused by heavy loads of nitrogen and phosphorus, the biological conditions of the Baltic Sea have been disturbed: large sea bottom areas without any biological life, low stocks of cods, and toxic blue green algaes. It is recognized that the nitrogen and phosphorus loads to the Baltic Sea must be reduced by 50% in order to restore the sea. The main purpose of this paper is to calculate cost effective nitrogen and phosphorus reductions to the Baltic Sea from the nine countries surrounding the Baltic Sea. The results show a significant difference in minimum costs of decreasing nitrogen and phosphorus loads to the Sea: approximately 12 000 millions of SEK per year and 3 000 millions of SEK respectively for reductions by 50%. It is also shown that a change from a policy of cost-effective nutrient reductions to a policy where each country reduces the nutrient loads by 50% increase total costs for both nitrogen and phosphorus reductions by about 300%. The results are, however, sensitive to several of the underlying assumptions and should therefore be interpreted with much caution.
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  • 13
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    Water, air & soil pollution 99 (1997), S. 477-486 
    ISSN: 1573-2932
    Keywords: sediment ; nitrogen ; phosphorus ; organic matter ; cluster analysis ; Gulf of Finland ; estuaries
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Dry weight (DW), ignition loss (IL) and concentrations of total nitrogen (TN) and total phosphorus (TP) of the sediment surface layer (0 to 10 cm, 1 cm slices) were analyzed from 20 sites in the eastern Gulf of Finland. The distance of the sampling sites from the mouth of the River Neva explained the nutrient concentrations of the sediments well, while the effect of water depth was negligible. The increase of TN and the decrease of TP along the transect from the river mouth towards the open Gulf were caused by the diminishing share of allochthonous material supplied from the River Neva. The mean TN concentration of the different accumulation areas was about 40 % higher in the sediment surface than in the deeper layer (9 to 10 cm). The corresponding difference for TP varied from 53 to 56 %. The results suggest considerable netflux of nutrients from sediment to water. The net sediment accumulation of nutrients were estimated as 6.0 g m-2 a-1 of N and 1.7 g m-2 a-1 of P corresponding 22 000 t a-1 of N and 6 100 t a-1 of P for the whole eastern Gulf.
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  • 14
    ISSN: 1573-2932
    Keywords: Adriatic Sea ; nutrients ; benthic fluxes ; carbon ; nitrogen ; silicon ; phosphorus ; budgets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Benthic fluxes of dissolved inorganic N, Si and P nutrients, alkalinity, dissolved inorganic C (DIC), and O2 from sediments in the Gulf of Trieste (northern Adriatic, Italy) were measured monthly in the period September 1995 – August 1996 using in situ incubated light benthic chambers. The highest efluxes of DIC, NH4 +, PO4 3−, Si(OH)4, and NO3 − influxes encountered in late summer — early autumn were the consequence of degradation of benthic microalgae, and in autumn mostly of sedimented phytoplankton. High NO3 − efflux was observed in spring. Only NH4 + and Si(OH)4 fluxes were significantly correlated with temperature. This correlation suggests that the rate of downward input and the quality and quantity of sedimentary organic matter (autochthonous and allochthonous) were superimposed on the temperature fluctuations. High DIC, NH4 + and Si(OH)4 effluxes observed in July 1996 were due to the late spring — early summer degradation of sedimentary organic matter produced by benthic microalgae, while the autumn phytoplankton bloom was quickly reflected in enhanced benthic fluxes due to higher temperature. Significant correlations between NH4 +, PO4 3− and Si(OH)4 fluxes suggested their parallel regeneration and utilization at the sediment-water interface. The nutrient fluxes were linked to O2 consumption, suggesting that aerobic oxidation processes were important at the sediment-water interface in the Gulf. The N, P and Si nutrients released from sediment pore waters are probably utilized in benthic microalgal and bottom-water primary production. This indicates that pelagic and benthic communities in the central part of the Gulf of Trieste function relatively independently of each other.
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  • 15
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    Water, air & soil pollution 98 (1997), S. 389-399 
    ISSN: 1573-2932
    Keywords: fertilizer ; nitrogen ; pollution ; runoff ; stable isotopes ; sugarcane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In many forested wetlands of Louisiana, surface water quality is being deteriorated by nutrient input from adjacent agricultural production area. This field study assesses the input of fertilizer N, applied to sugarcane fields, to forested wetlands. The potential use of natural abundance variations in15N/14N ratios for identification and tracing surface water N sources (NH 4 + - and NO 3 − -N) was evaluated. Runoff and surface water samples were collected from sugarcane fields and bordering forested wetlands (6 stations) over a 16 month period and analyzed for NH 4 + -N, NO 3 − -N, and associated NH 4 + -δ 15N and NO 3 − -δ 15N ratios. FertilizerN draining into adjacent forested wetland was estimated to be only a small fraction of the amount applied. Concentrations of NH 4 + - and NO 3 − -N in the collected water samples were low and ranged from 0.02 to 1.79 mg L−1. Isotopic analysis revealed NH 4 + -δ 15N and NO 3 − -δ 15N means were distinctive and may have the potential to be used as tracers of N contamination. The mean NH 4 + -δ 15N value was +18.6±7.1‰ and the NO 3 − -δ 15N mean was +8.3±3.1‰. Anomalously high NO 3 − -δ 15N values (〉30‰) were attributed to denitrification.
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  • 16
    ISSN: 1573-5028
    Keywords: chromosome location ; linolenic acid ; microsome ; ω-3 fatty acid desaturase ; Oryza sativa ; transgenic hairy roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ω-3 fatty acid desaturases are membrane-bound enzymes catalyzing the conversion of linoleic acid to linolenic acid in lipids, and are located both in the microsome and plastid envelopes as two different isoforms. A cDNA encoding the microsome ω-3 fatty acid desaturase (OsFAD3) and the corresponding genomic clone were isolated from rice (Oryza sativa L.). The OsFAD3 gene was composed of 8 exons and 7 introns. A microsatellite was present in the second exon of the OsFAD3 gene, showing polymorphism between Indica and Japonica rice varieties. The mapping of this microsatellite showed that the OsFAD3 gene was located on chromosome 11. Expression of the OsFAD3 cDNA in tobacco hairy root tissues and subsequent analysis of fatty acid compositions demonstrated the activity of the microsome ω-3 fatty acid desaturase. The OsFAD3 mRNA was abundant in root tissues, but was hardly detectable in leaves. In root tissues, a high level of the OsFAD3 mRNA was observed at 15 °C and 20 °C, with its level decreasing markedly at temperatures below 10 °C. The accumulation of the OsFAD3 mRNA in leaf tissues remained at quite low levels, both at normal growth temperatures and at chilling temperatures. Similar temperature responses of the OsFAD3 gene were observed both in chilling- tolerant and in chilling-intolerant rice cultivars.
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  • 17
    ISSN: 1573-5028
    Keywords: arginine decarboxylase ; gene expression ; Oryza sativa ; polyamines ; rice ; salinity stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of salinity stress on the activity of arginine decarboxylase (ADC, EC 4.1.1.19), the first enzyme in biosynthesis of polyamines (PA) from arginine, as well as its transcript level has been compared in salt-sensitive (M-1-48) and salt-tolerant (Pokkali) rice cultivars. Treatment of 72 h grown seedlings either with increasing concentrations of NaCl or with 150 mM NaCl for different time periods, showed a gradual increase of activity in Pokkali. In M-1-48 an immediate increase followed by sharp decrease was observed on prolonged treatment beyond 6 h or above 150 mM NaCl. To generate a DNA probe for ADC, the polymerase chain reaction was used with oat genomic DNA and sequence-specific primers. A region of oat genomic DNA containing a coding sequence for 166 amino acids of the C-terminal part of the ADC enzyme was amplified and called OAD1. Southern analysis of EcoRI- or BamHI-cut genomic DNAs from different cultivars of rice with OAD1 as the probe revealed strong hybridization with one DNA fragment of rice and restriction fragment length polymorphism (RFLP) was noticed. Northern analysis of total RNA of rice with OAD1 as the probe revealed hybridization with a transcript of similar size to the ADC transcript in oat. While in Pokkali, at least a 20-fold accumulation of OAD1 homologous transcript was detected after treatment with 200 mM NaCl, only a seven-fold increase in transcript level was found in M-1-48 after 150 mM NaCl treatment. Results suggest that in the salt-tolerant rice cultivar Pokkali, ADC enzyme activity increases and its transcript also accumulates during the prolonged salinity stress, this mechanism is absent in the salt-sensitive rice cultivar M-1-48 where a prolonged period of salinity stress down-regulates both ADC activity and its transcript level.
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  • 18
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    Theoretical and applied genetics 94 (1997), S. 316-321 
    ISSN: 1432-2242
    Keywords: Key words Weedy rice ; Oryza sativa ; Classification ; Origin of weedy rice ; Isozyme ; Randomly amplified polymorphic DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Weedy rice (Oryza sativa L.) is an important resource for breeding and for studying the evolution of rice. The present study was carried out to identify the genetic basis of the weedy rices distributed in various countries of the world. One hundred and fifty two strains of weedy rice collected from Bangladesh, Brazil, Bhutan, China, India, Japan, Korea, Nepal, Thailand and the USA were tested for variations in six morpho-physiological characteristics and in 14 isozyme loci. Twenty six weedy strains selected from the above materials were assayed for the Est-10 locus, six RAPD loci of the nuclear genome, and one chloroplast locus. From the results of multivariate analysis based on the morpho-physiological characteristics and the isozymes, weedy rice strains were classified into indica and japonica types, and each type was further divided into forms resembling cultivated and wild rice. Thus, four groups designated as I, II, III and IV were identified. Weedy strains of group I (indica-type similar to cultivars) were distributed mostly in temperate countries, group II (indica-type similar to wild rice) in tropical countries, group III (japonica-type similar to cultivars) in Bhutan and Korea, group IV ( japonica-type similar to wild rice) in China and Korea. In group I, classified as indica, several strains showed japonica-specific RAPD markers, while some others had japonica cytoplasm with indica-specific RAPD markers in a heterozygous state at several loci. One weedy strain belonging to group II showed a wild rice-specific allele at the Est-10 locus. However, in groups III and IV, no variation was ound either for the markers on Est-10 or for the RAPD loci tested. Judging from this study, weedy rice of group I might have originated at least partly from gene flow between indica and japonica, whereas that of group II most probably originated from gene flow between wild and cultivated indica rice. Weedy rice of group III is thought to have originated from old rice cultivars which had reverted to a weedy form, and that of group IV from gene flow between japonica cultivars and wild rice having japonica backgrounds.
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  • 19
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    Theoretical and applied genetics 95 (1997), S. 359-368 
    ISSN: 1432-2242
    Keywords: Key words Retroposon ; SINE ; Target site duplication ; Oryza sativa ; AA genome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We determined copy numbers of p-SINE1, a short interspersed element (SINE) in rice, and found that p-SINE1 was distributed in the Oryza sativa genome with an average spacing of about 1 member per 70 kb. We identified 31 p-SINE1 members located at different loci by cloning and characterized them by sequencing. Comparison with a consensus sequence derived from their sequences revealed that all of the p-SINE1 members contained base substitution mutations at various positions. In addition to the substitutions, some members contained deletions, insertions and tandem duplications of a few bases or of a large DNA segment. These mutations seem to have occurred to inhibit transcription from each p-SINE1 member by RNA polymerase III. PCR using a pair of primers that hybridize with the sequences flanking each p-SINE1 member revealed that many of the p-SINE1 members were present at corresponding loci in strains belonging to all rice species carrying the AA genome. Several of them were, however, present at corresponding loci in strains belonging to a limited number of species or in a limited number of the strains belonging to a rice species. These p-SINE1 members are supposed to be useful in the identification and classification of various rice strains with the AA genome. Simple tandem repeats of a trinucleotide (CAT) or dinucleotide (AG) sequence existed in the flanking regions of 2 p-SINE1 members. Such repeats, called microsatellite DNA, varied in number even in the cultivated rice strains examined, suggesting that microsatellite DNA is useful for the identification and classification of cultivars.
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  • 20
    ISSN: 1432-2242
    Keywords: Key words RFLP ; Insect resistance ; Oryza sativa ; Orseolia oryzae ; Linkage analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A PCR-based marker (E20570) linked to the gene Gm4t, which confers resistance to a dipteran pest gall midge (Orseolia oryzae), has been mapped using the restriction fragment length polymorphism (RFLP) technique in rice. Gm4t is a dominant resistance gene. We initially failed to detect useful polymorphism for this marker in a F3 mapping population derived from a cross between two indica parents, ‘Abhaya’בShyamala’, with as many as 35 restriction enzymes. ‘Abhaya’ carries the resistance gene Gm4t and ‘Shyamala’ is susceptible to gall midge. Subsequently, E20570 was mapped using another mapping population represented by a F2 progeny from a cross between ‘Nipponbare’, a japonica variety, and ‘Kasalath’, an indica variety, in which the gene Gm4t was not known to be present. Gm4t mapped onto chromosome 8 between markers R1813 and S1633B. Our method, thus, presents an alternative way of mapping genes which otherwise would be difficult to map because of a lack of polymorphism between closely related parents differing in desired agronomic traits.
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  • 21
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    Theoretical and applied genetics 95 (1997), S. 112-118 
    ISSN: 1432-2242
    Keywords: Key words Diallel cross ; Hybrid rice ; Oryza sativa ; Restriction fragment length polymorphism (RFLP) ; Simple sequence repeat (SSR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The partial sterility of hybrids between the indica and japonica rice subspecies of Asian cultivated rice is a serious constraint for utilizing inter-subspecific heterosis in hybrid rice breeding. In this study, we have investigated the relationship between molecular-marker polymorphism and indica-japonica hybrid fertility using a diallel set involving 20 rice accessions including 9 indica and 11 japonica varieties. Spikelet fertility of the resulting 190 F1s and their parents was examined in a replicated field trial. Intra-subspecific hybrids showed much higher spikelet fertility than inter-subspecific hybrids except in crosses involving wide-compatibility varieties. The parents were surveyed for DNA polymorphism using 96 RFLP and ten SSR markers, which revealed extensive genetic differentiation between indica and japonica varieties. A large number of markers detected highly significant effects on hybrid fertility. The chromosomal locations for many of the positive markers coincided well with previously identified loci for hybrid sterility. The correlation between hybrid fertility and parental distance was low in both intra- and inter-subspecific crosses. The results suggest that the genetic basis of indica-japonica hybrid sterility is complex. It is the qualitative, rather than the quantitative, difference between the parents that determines the fertility of hybrids.
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  • 22
    ISSN: 1610-7403
    Keywords: litterbag ; mass loss ; microclimate ; microcosms ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The decay rates of Japanese Konara Oak (Quercus serrata Murray) and Japanese Red Pine (Pinus densiflora Sieb. et Zucc.) leaf litter were monitored for one year. It aimed to compare the decomposition of leaf litter using microcosms set up in the field (FM) and in the greenhouse (GM), with the litterbag (LB) method as control. Results showed that incubation setting affected the decay rate (k), respiration rates and the changes in the concentrations of nitrogen (N). Thek value ofQuercus in FM was higher than LB, while thek value ofPinus was higher in the LB than in FM. The decay ratesk for both species, however, were significantly lower in GM than FM and LB, clearly suggesting that decay rate was inhibited in the greenhouse. Significant differences in microclimatic variables and soil biological activities (soil respiration) existed between greenhouse and field microcosms, hence, the decay rates were affected. The N concentrations for both litter types increased as decomposition proceeded. Decomposition studies using laboratory microcosm approach alone may lead to erroneous conclusions especially if no appropriate field studies are conducted along with it.
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  • 23
    ISSN: 1573-5052
    Keywords: Aster ; Atriplex ; Chenopodium ; nitrogen ; phosphorus ; roots ; shoots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The loss of dry mass, nitrogen and phosphorus from shoot and root litter of mudflat annuals was examined in a series of experimental marshes in the Delta Marsh, Manitoba, Canada. Litter bags containing shoot material of three mudflat annuals (Aster laurentianus Fern., Atriplex patula L., and Chenopodium rubrum L.) were placed on the sediment surface of the marshes under drawdown conditions. In addition, litter bags containing root material of these three species were shallowly buried. Approximately 70% and 50% of both shoot and root litter, respectively, was still present after one year in the field. During the second year when the marshes were flooded, shoot and root litter lost an additional 20% and 0% of their mass, respectively. Except for Chenopodium roots, which accumulated nitrogen and phosphorus during both years, shoot and root litter lost from 0 to 50% of their nitrogen and phosphorus early in the first year, with levels generally remaining constant through the remainder of the study period. Our results indicate that mudflat annual litter decomposed slowly and would provide abundant habitat for aquatic invertebrates when these marshes were reflooded. However, most nutrient loss took place in the first year when the litter was unflooded, with little loss occurring in the second year when flooded.
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  • 24
    ISSN: 1572-9788
    Keywords: diversity ; genetic mapping ; Oryza sativa ; RAPDs ; rice ; inter-simple sequence repeats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic variation between samples of Oryza sativa from 19 localities in Bangladesh and Bhutan was assessed using two PCR-based molecular marker systems: RAPD (random amplification of polymorphic DNA) and ISSR-PCR (inter-simple sequence repeat polymerase chain reaction). Employing RAPD, a set of 14 decanucleotides of arbitrary sequence directed the amplification of 94 reproducible marker bands, 47 (50%) of which were polymorphic. In addition, a set of 9 ISSR primers were used to direct amplification of 71 PCR products, 40 (56%) of which were polymorphic. Multivariate analyses of the two PCR-based molecular marker data sets provided evidence that the patterns of variation correspond with the classification described by Glaszmann [9] using isozyme analysis. Subtle differences in the relationships revealed between rice groups using the two types of PCR-based marker led to investigations of their map positions using an intraspecific doubled haploid mapping population. The observation that the chromosomal locations of markers can influence diversity assessments is presented and the significance of this is discussed.
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  • 25
    ISSN: 1573-5117
    Keywords: fish food ; faeces ; nutrient ; nitrogen ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fish food and faeces were fractioned into the differentcomponents of phosphorus and nitrogen. There was a rapid release ofphosphorus from the fish food and faeces and a decrease thereafterwhereas ammonium release was slow at first with the rate increasingwith time. Both temperature and pH affected the release of nutrientsfrom fish food and faeces. The release of phosphorus and nitrogen washigher at higher temperatures. The maximum release of phosphorus wasat pH 4.0 whereas nitrogen release was maximum at neutral (7.0) toalkaline (10.0) media.
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  • 26
    ISSN: 1573-5117
    Keywords: nitrogen ; phosphorus ; drainage waters ; sediments ; sorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lake Łuknajno, a shallow (max. depth 3 m) and relativelylarge(630 ha) water body situated in the Great Masurian Lakessystem(Poland) is strongly affected by its agricultural watershed.Thetotal volume of drainage waters pumped into the lake in 1993amounted 2.84 × 106m3 which is equivalentto65% of the lake volume. In spring and autumn drainage waterswereextremely rich in nitrogen (especially nitrates) and the N:Pratioreached 57 in spring. In summer, drainage waters containedrelatively more phosphorus so the N:P ratio decreased tonearly 10.Nutrient concentrations in lake water followed the changes ininflowing waters. High concentrations of nitrates and ammoniawerenoted in lake water in spring but summer concentrations oftheseions were close to zero. Soluble reactive phosphorus variedbetween10 and 20 µg P l−1 throughout the season. A constantoutflow of nitrogen to the lower Lake Śniardwy was assumedbased ona permanent concentration gradient between waters of these twolakes.Lake Łuknajno is a hard water lake. Co-precipitation ofphosphorus with calcium carbonate is likely to occur though nosignificant P accumulation in bottom sediments was found.Sorptionof phosphorus on sediments as measured under experimentalconditions has minor effects on P cycling. Bottom sedimentscomposed mainly of calcareous gyttja do not accumulate eitherorganic matter or mineral forms of nutrients.
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  • 27
    ISSN: 1573-5117
    Keywords: pristine ; hydrochemistry ; podzol ; soil processes ; sulphate ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During the Surface Water Acidification Programme, ahydrochemical plot study was carried out at thepristine Høylandet study site during 1986–89. Theplot soils were acidic iron podzols (sensuKubiena), with a significant content of secondaryaluminium (Al), forming a potential major source oftoxic Al in streams and lakes. Rain and mist inputscontain small amounts of anthropogenic sulphate, withammonium inputs being enhanced during the summermonths. Vegetation canopy interactions, includingsorption and leaching, change input water chemistrybefore it reaches the soil, in which organicinteractions increase, especially during the summer.Amphibole in the soil parent material is probablyresponsible for the large mineral weathering ratedetermined and this source of base cations, alliedwith the small anthropogenic inputs, results in Alconcentrations in the streamwater being small incomparison with impacted sites.
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  • 28
    ISSN: 1573-5117
    Keywords: sediment–water fluxes ; nitrogen ; chlorophyll a ; derivatives ; seasonal variations ; estuaries ; Mediterranean Coast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seasonal variability of sediment–water ammonium flux andoxygen uptake was studied in an estuarine bay (Alfacs Bay, Ebro Delta, NWMediterranean) influenced by temporal freshwater discharges. Three stationswith different organic loading were sampled. The relationships of benthicfluxes to bottom water (temperature, dissolved oxygen, ammonium, nitrateplus nitrite) and to sediment (porosity, chlorophyll a derivative pigments,organic carbon and nitrogen) variables were examined. Oxygen uptake rangedfrom 0.3 to 2.5 mmol m−2 h−1 and ammoniumrelease ranged from 6 to 230 µmol m−2 h−1.The lowest value was recorded at the station furthest from the freshwaterinputs, and the highest was at the littoral station nearest the freshwaterdischarge channels (for oxygen uptake) and at the deep station at the saltwedge front (for ammonium flux). Water temperature and the concentration ofchlorophyll a derivative pigments on the surface sediment were revealed asthe main variables to be taken into account to explain the variabilityfound. Changes in fluxes reflecting temperature changes were found at thestation furthest from the freshwater inputs, while at the other, fluxvariability was found to be related to the cycle of functioning offreshwater discharge channels. The different patterns of variability arediscussed in relation to the dynamics of the estuary and to the mainfeatures of benthic nitrogen cycling.
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  • 29
    ISSN: 1573-5117
    Keywords: lake sediment ; bioturbation ; chironomid larvae ; organic matter decomposition ; nutrient exchange ; nitrogen ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The importance of Chironomus plumosus larvae onbenthic metabolism and nutrient exchange across thesediment–water interface was evaluated in a shalloweutrophic lake (Lake Arreskov, Denmark) following aphytoplankton sedimentation. Chironomus plumosuslarvae were added to laboratory sediment microcosms,corresponding to a density of 2825 larvae m−2.Non-inhabited microcosms served as controls. Asedimentation pulse of organic matter was simulated byadding fresh algal material (Chlamydomonasreinhardii) to sediment cores (36 g dryweight m−2). The mineralization was followed bymeasuring fluxes of O2, CO2, dissolvedinorganic nitrogen and phosphate. A rapid clearance ofalgae from the water column in faunated microcosmssuggested that chironomids may be of major importancein controlling phytoplankton concentrations in shalloweutrophic lakes. Chironomids increased the sedimentO2 uptake ≈ 3 times more than what wouldbe expected from their own respiration, indicating astimulation of microbial activity and decomposition oforganic matter in the sediment. Addition of algaeenhanced the release of CO2, NH+ 4 ando-P. The excess inorganic C, N and P released inamended non-inhabited sediment after 36 dayscorresponded to 65, 31 and 58% of the C, N and P inthe added algae. In sediment inhabited by Chironomus plumosus the corresponding numbers were147, 45 and 73%, indicating that mineralization oforganic matter also from the indigenous sediment poolwas stimulated by chironomids.
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  • 30
    ISSN: 1573-5117
    Keywords: wastewater ; nitrogen ; phosphate ; constructed wetlands ; Phragmites mauritianus ; mesocosms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Artificial wetlands have not yet been used in Ugandafor wastewater purification. The feasibility of usingartificial wetlands to improve presettled wastewaterquality was tested using 40 l experimental buckets(mesocosms) at Kirinya sewage works, Jinja, Uganda.These mesocosms were operated in an intermittent,vertical flow mode. They were synoptic experiments toa pilot constructed wetland project in which theutility of Phragmites mauritianus to treatpresettled wastewater for P and N was examined. Results showed that the laterite-gravel rootedPhragmites reactors improved the wastewater qualitysignificantly, possibly to advanced secondary ortertiary water quality levels. These reactors achievedreduction efficiency of over 90% for P and over 60%for N after a 5-day water retention time. The massbalances of N and P over the reactors, indicated ahigher uptake rate for P by the plant than for N.Laterite-gravel rooted Phragmites mesocosms weremore efficient than the floating Phragmitesmesocosms for the wastewater treatment.
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  • 31
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    Plant molecular biology 35 (1997), S. 145-153 
    ISSN: 1573-5028
    Keywords: complex traits ; linkage ; molecular marker ; Oryza sativa ; positional cloning ; QTL analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Recent progress in the generation of a molecular genetic map and markers for rice has made possible a new phase of mapping individual genes associated with complex traits. This type of analysis is often referred to as quantitative trait locus (QTL) analysis. Increasing numbers of QTL analyses are providing enormous amounts of information about QTLs, such as the numbers of loci involved, their chromosomal locations and gene effects. Clarification of genetic bases of complex traits has a big impact not only on fundamental research on rice plant development, but it also has practical benefits for rice breeding. In this review, we summarize recent progress of QTL analysis of several complex traits in rice. A strategy for positional cloning of genes at QTLs is also discussed.
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  • 32
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    European journal of plant pathology 103 (1997), S. 747-750 
    ISSN: 1573-8469
    Keywords: Appressoria ; fungitoxicity ; Magnaporthe grisea ; Oryza sativa ; partial resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 33
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    Molecular genetics and genomics 253 (1997), S. 615-623 
    ISSN: 1617-4623
    Keywords: Key words Flower development ; MADS box genes ; Oryza sativa ; Sorghum bicolor ; Synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract With the aim of elucidating the complex genetic system controlling flower morphogenesis in cereals, we have characterized two rice and two sorghum MADS box genes isolated from cDNA libraries made from developing inflorescences. The rice clones OsMADS24 and OsMADS45, which share high homology with the Arabidopsis AGL2 and AGL4 MADS box genes, are expressed in the floral meristem, in all the primordia, and in mature floral organs. High expression levels have also been found in developing kernels. The sorghum clone SbMADS1 is also homologous to AGL2 and AGL4: expression analysis and mapping data suggest that it is the ortholog of OsMADS24. The pattern of expression of SbMADS2, the other sorghum MADS box gene, suggests that it may play a role as a meristem identity gene, as does AP1 in Arabidopsis, to which it shows considerable homology. The four genes have been mapped on a rice RFLP genetic map: the results are discussed in terms of synteny among cereals.
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  • 34
    ISSN: 1573-5036
    Keywords: eutrophication ; herons ; nesting site ; nitrogen ; nitrophyllous species ; phosphorus ; similarity index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effects of colony nesting of herons on soil properties and herb layer composition in Pinus densiflora forest were studied at Pomaeri, Kangwon Province in Korea. Herons have used this habitat as a nesting site from January to October every year. In 1995, more than 500 herons were observed in this habitat. Nutrient content of soil was much higher at the nesting site than that of the non-nesting site (control). Total nitrogen concentration of soil at the nesting site and the control site was 14.8±1.85 mg g-1 and 2.8±0.35 mg g-1, respectively. Phosphorus content of soil in the nesting site was 32 times greater than that of the control site. This is evidently due to the addition of feces of the herons, and decomposition of thin twigs and organic debris from the canopy of dead trees and bird nests. Light intensity at herb layer of the nesting site and of the control site was 80% and 20%, respectively, of incident on outside forest. Species diversity of herb layer in the nesting site (9 species) was quite lower than that in the control site (14 species). Similarity index of the herb layer between the two sites was 0.07. The nesting site was dominated by indicator species of soil eutrophication such as Humulus japonicus, Persicaria perfoliata, Persicaria fauriei, Commelina communis, Chelidonium majus var. asiaticum. Changes of herb species composition in the nesting site was evidently due to the eutrophication of the soil and increased light intensity of the herb layer.
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  • 35
    ISSN: 1573-5036
    Keywords: DRIS ; nitrogen ; perennial ryegrass ; phosphorus ; potassium ; sulphur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Herbage analysis offers a definitive means of determining the N, P, K and S status of perennial ryegrass swards. Unfortunately, the results of such analyses can be difficult to interpret, simply because the minimum or 'critical' concentration of a nutrient in plant tissue for optimum growth, varies both with crop age and with changes in the concentrations of other nutrients. The Diagnosis and Recommendation Integrated System (DRIS) could help to improve the reliability of such interpretations. Diagnoses made using DRIS are based on relative rather than on absolute concentrations of nutrients in plant tissue, and as such should be comparatively independent of crop age. The aim of this study was to establish and test DRIS methodology for high-yielding perennial ryegrass swards. Because of prohibitive costs, setting up a whole new series of field experiments to evaluate DRIS model parameters for perennial ryegrass was out of the question. Instead, the diagnostic norms and associated coefficients of variation for the model were evaluated using data from a single (large) multi-factorial glasshouse experiment. Of the nutrient ratios selected to form the diagnostic norms, K/N and S/N had the clearest physiological rationale, whereas those involving Ca and Mg in combination with N, P, K and S appeared to have little physiological basis. It was reasoned, though, that because Ca and Mg uptake by plants are largely passive processes (ultimately governed by plant growth), the DRIS indices for these nutrients, together reflected the degree to which growth may be limited by non-nutritional (environmental) factors relative to nutritional ones. Both indices were combined to form a single reference (Ri) index. Without such an internal reference, plant growth could be limited by multiple nutrient deficiencies, and yet N, P, K and S indices might all be close to, or equal to zero (i.e. the optimum), simply because the absolute concentrations of each nutrient (while low) had been in the correct state of balance. Moreover, by effectively using Ca and Mg as internal reference parameters in DRIS, 'nutrient concentrations' which previously formed the basis of the critical value approach, were essentially incorporated into the DRIS model, thus combining the strengths of the two diagnostic approaches; the only difference being that Ca and Mg, and not dry matter, were the internal references against which the levels of the major nutrients were compared.
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  • 36
    ISSN: 1573-5036
    Keywords: ammonium toxicity ; carbohydrates ; CO2 ; nitrogen ; Pinus taeda ; Pinus ponderosa ; root respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We measured CO2 efflux from intact root/rhizosphere systems of 155 day old loblolly (Pinus taeda L.) and ponderosa (Pinus ponderosa Dougl. ex Laws.) pine seedlings in order to study the effects of elevated atmospheric CO2 on the below-ground carbon balance of coniferous tree seedlings. Seedlings were grown in sterilized sand culture, watered daily with either 1, 3.5 or 7 mt M NH 4 + , and maintained in an atmosphere of either 35 or 70 Pa CO2. Carbon dioxide efflux (μmol CO2 plant−1 s−1) from the root/rhizosphere system of both species significantly increased when seedlings were grown in elevated CO2, primarily due to large increases in root mass. Specific CO2 efflux (μmol CO2 g root−1 s−1) responded to CO2 only under conditions of adequate soil nitrogen availability (3.5 mt M). Under these conditions, CO2 efflux rates from loblolly pine increased 70% from 0.0089 to 0.0151 μmol g−1 s−1 with elevated CO2 while ponderosa pine responded with a 59% decrease, from 0.0187 to 0.0077 μmol g−1 s−1. Although below ground CO2 efflux from seedlings grown in either sub-optimal (1 mt M) or supra-optimal (7 mt M) nitrogen availability did not respond to CO2, there was a significant nitrogen treatment effect. Seedlings grown in supra-optimal soil nitrogen had significantly increased specific CO2 efflux rates, and significantly lower total biomass compared to either of the other two nitrogen treatments. These results indicate that carbon losses from the root/rhizosphere systems are responsive to environmental resource availability, that the magnitude and direction of these responses are species dependent, and may lead to significantly different effects on whole plant carbon balance of these two forest tree species.
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  • 37
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    Plant and soil 195 (1997), S. 351-364 
    ISSN: 1573-5036
    Keywords: barrier island ; environmental effects ; litter quality ; nitrogen ; phosphorus ; root decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A root decomposition study using the litterbag approach was conducted along a dune and swale chronosequence on the Virginia Coast Reserve-Long Term Ecological Research Site in Virginia, USA to evaluate how environmental and substrate quality factors influence belowground decay and associated nutrient dynamics. Gradients in moisture levels and nitrogen availability associated with the chronosequence provided the experimental framework. Spartina patens roots were buried at all sites as a standard substrate to evaluate environmental influences. Roots native to each site were buried to evaluate community decay dynamics and the influence of litter quality. Spartina decay was reduced in the wet, anoxic soils of swale sites (k = 0.21–0.33 yr-1) relative to decay in dunes soils (k = 0.52–0.72 yr-1). Increasing soil nitrogen availability from younger to older sites had no effect on the rate of Spartina root decay. Native root decay across the Hog Island chronosequence exhibits certain trends expected in response to nitrogen limitation and moisture availability. Increased nitrogen content of root material corresponds to increased soil nitrogen availability. Among dune sites, native root decay increased in concert with increased root nitrogen (6 year k = 0.34 yr-1, 120 year dune: k = 0.97 yr-1). Litter quality, alone, does not explain this trend since Spartina roots decayed more slowly than native dune roots and had a higher initial nitrogen content. Among swales, increased moisture levels and associated soil anoxia inhibited native root decomposition and minimized the effects of litter quality on decay. In general, phosphorus was rapidly lost from decaying roots while nitrogen immobilization was low to nonexistent. The low nitrogen immobilization of decaying roots in a nitrogen limited ecosystem warrants further study and may reveal that belowground decay increases the rate of nutrient cycling relative to decay aboveground.
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  • 38
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    Euphytica 95 (1997), S. 45-48 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; non-allelic ; segregation ; semidwarfism ; sd1 gene ; dwarf ; mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The mode of inheritance of five semidwarf (SD) mutants and allelic relationship with DGWG (sd1) locus was studied. The five SD mutants viz., Basmati 370 (SD1), Basmati 370 (SD2), Basmati 370 (SD3), TCA 2 (SD) and TCA P2-5 (SD) when crossed with their tall parents exhibited monogenic inheritance of 3 tall: 1 semidwarf in the F2 progenies. The SD mutants were also crossed with semidwarf varieties (DGWG and Bala) possessing sd1 gene. Crosses between Basmati 370 (SD1) × Bala, Basmati 370 (SD3) × Bala and TCA 2 (SD) × DGWG produced tall F1 hybrids and in F2 generation, modified dihybrid ratios (9 : 6 : 1 and 9 : 7) were observed indicating that the mutants Basmati 370 (SD1), Basmati 370 (SD3) and TCA 2 (SD) are non-allelic to sd1 gene. Whereas, the mutants Basmati 370 (SD2) and TCA P2-5 (SD) when crossed with Bala produced semidwarf F1 hybrids and in F2 generation, segregation for plant height was not observed indicating that these mutants are allelic to sd1 gene. The three non-allelic SD mutants identified in the present study can be used in rice breeding as alternative gene sources for semidwarfism.
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  • 39
    ISSN: 1573-5036
    Keywords: catch crop ; mineralisation ; nitrogen ; simulation model ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The release of nitrogen from incorporated catch crop material in winter is strongly influenced by soil temperatures. A laboratory experiment was carried out to investigate this influence in the range of 1-15 °C. Samples of sandy soil or a mixture of sandy soil with rye shoots were incubated at 1-5-10-15 °C, and samples of sandy soil with rye roots were incubated at 5-10-15 °C. Concentrations of Nmin (NH4 +-N and NO3 --N) were measured after 0-1-2-4-7-10 weeks for the sandy soil and the sandy soil:rye shoot mixture, and after 0-2-7-10 weeks for the sandy soil:rye root mixture. At 1 °C, 20% of total organic N in the crop material had been mineralised after ten weeks, indicating that mineralisation at low temperatures is not negligible. Maximum mineralisation occurred at 15 °C; after ten weeks, it was 39% of total applied organic nitrogen from shoot and 35% from root material. The time course of mineralisation was calculated using an exponential decay function. It was found that the influence of temperature in the range 1-15 °C could be described by the Arrhenius equation, stating a linear increase of ln(k) with T-1, k being the relative mineralisation rate in day-1 and T the temperature (°C). A simulation model was developed in which decomposition, mineralisation and nitrification were modelled as one step processes, following first order kinetics. The relative decomposition rate was influenced by soil temperature and soil moisture content, and the mineralisation of N was calculated from the decomposition of C, the C to N ratio of the catch crop material and the C to N ratio of the microbial biomass. The model was validated first with the results of the experiment. The model was further validated with the results of an independent field experiment, with temperatures fluctuating between 3 and 20 °C. The simulated time course of mineralisation differed significantly from the experimental values, due to an underestimation of the mineralisation during the first weeks of incubation.
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  • 40
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    Plant and soil 191 (1997), S. 279-290 
    ISSN: 1573-5036
    Keywords: acidification ; carbon ; carbon dioxide ; nitrogen ; pasture ; Pinus radiata ; sea salt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The conversion of hill country pasture to exotic forest plantations is occurring rapidly (70,000 ha yr−1) in New Zealand. Impacts of this land-use change on soil properties, soil fertility, and water quality are only beginning to be investigated. This study examines the effects of radiata pine (Pinus radiata) on soil and soil solution chemistry, in a region of low atmospheric pollution, 20 years after plantation establishment, assuming that the pasture and pine research sites had comparable soil properties before planting pine. The primary effects of conversion on soil chemistry were a decrease of organic carbon in the mineral soil that was balanced by an accumulation of the surface litter layer, a decrease in soil N, soil acidification, and increased pools of exchangeable Mg, K, and Na. Soil solution studies revealed a large input of sea salts by enhanced canopy capture of sea salts that contributed to much larger solute concentrations and elemental fluxes in the pine soil. Sea salts appear to accumulate in the micropores of pine soil during the dry summer period and are slowly released to macropore flow during the rainy season. This results in a progressive decrease in solute concentrations over the period of active leaching. While chloride originating from sea salt deposition was the dominant anion in the pine soil, bicarbonate originating from root and microbial respiration was the dominant anion in the pasture soil. Carbon dioxide concentrations in the soil atmosphere were 12.5-fold greater in the pasture soil than in the pine soil due to greater rates of root and microbial respiration and to slower diffusion rates resulting from wetter soil conditions in the pasture. Although elemental fluxes from the upper 20 cm of the soil profile were substantially greater in the pine soil, these losses were compensated for by increased elemental inputs resulting from nutrient cycling and enhanced canopy capture of sea salts.
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  • 41
    ISSN: 1573-5036
    Keywords: carbohydrates ; carbon dioxide ; nitrogen ; nutrients ; roots ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Rising atmospheric carbon dioxide, nitrogen deposition and warmer temperatures may alter the quantity and quality of plant-derived organic matter available to soil biota, potentially altering rates of belowground herbivory and decomposition. Our objective was to simulate future growth conditions for an early successional (loblolly) and late successional (ponderosa) species of pine to determine if the physical and chemical properties of the root systems would change. Seedlings were grown for 160 days in greenhouses at the Duke University Phytotron at 35 or 70 Pa CO2 partial pressure, ambient or ambient + 5 °C temperature, and 1 or 5 mMNH4O3. Roots from harvested seedlings were analyzed for changes in surface area, specific root length, mass, total nonstructural carbohydrates (TNC), and concentrations of macro-nutrients. Surface area increased in both species under elevated CO2, due primarily to increases in root length, and this response was greatest (+138%) in loblolly pine at high temperature. Specific root length decreased in loblolly pine at elevated CO2 but increases in mass more than compensated for this, resulting in net increases in total length. TNC was unaffected and nutrient concentrations decreased only slightly at elevated CO2, possibly from anatomical changes to the root tissues. We conclude that future growth conditions will enhance soil exploration by some species of pine, but root carbohydrate levels and nutrient concentrations will not be greatly affected, leaving rates of root herbivory and decomposition unaltered.
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  • 42
    ISSN: 1573-5036
    Keywords: nitrogen ; 13N ; 15N ; phloem sap ; positron-emitting tracer imaging system ; rice ; xylem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen movement through the xylem vessels and sieve tubes in rice plants was studied using xylem and phloem sap analysis in combination with stable and radioactive nitrogen isotope techniques. More than 90% of nitrogen was translocated in the sieve tubes of rice plants as amino acids. When 15N (99.6 atom%) was applied as a nitrate to the root, 15N first appeared in phloem sap of the leaf sheath within 10 minutes and increased to 37 atom% excess 5 hours after the experiment had started. In long-term experiments, 63% of nitrogen in the phloem sap of the leaf sheath and 15% in that of the uppermost internode came from nitrogen absorbed within the last 24 hours and 50 hours, respectively. To obtain information about the more rapid circulation of nitrogen in the plant, radioactive 13N was used as a tracer. A positron-emitting tracer imaging system was used to show that 13N was transferred to the leaf sheath within 8 minutes of its application to the roots. Analysis of the xylem sap of the leaf sheath showed that when the nitrate was applied to the roots, most of the nitrogen in the xylem was transported as a nitrate. These data showed that phloem and xylem sap analysis together with the stable and radioactive nitrogen techniques provide a good method for the detection of nitrogen cycles in plants.
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  • 43
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    Plant cell, tissue and organ culture 50 (1997), S. 13-18 
    ISSN: 1573-5044
    Keywords: Oryza sativa ; picolinic acid ; rice blast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An in vitro screening procedure was adapted to study the sensitivity of callus to the toxin picolinic acid of Pyricularia grisea in four rice cultivars. The lethal dose LD50 was determined on the basis of probit-log dosage response curve. The values of LD50 were 10, 51, 129 and 151 ppm for Tetep, Newbonnet, Labelle and M 201, respectively. The callus culture of cultivar Tetep, with a known broad spectrum of resistance, exhibited a high toxin sensitivity whereas the highly susceptible cultivar M 201 showed low sensitivity indicating the absence of relation between host plant specific resistance to blast and resistance of the callus to toxin.
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  • 44
    ISSN: 1573-5060
    Keywords: ‘contig lines’ ; interspecific hybridisation ; genetic resources ; molecular markers ; Oryza glaberrima ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the past, utilisation of African cultivated rice, Oryza glaberrima, in O. sativa breeding was hampered by high sterility in interspecific F1 and in early progenies. Recent development of mapped molecular markers provides the opportunity to rationalise and to monitor introgressions between the two cultivated rice species. Development of such introgressed lines through interspecific hybridisation and backcrossing represents high potential to create new genetic and transgressive variation. We are initiating an original marker-aided backcross program with the aim of producing a set of 100 ‘contig lines’, each one bearing an alien O. glaberrima chromosomal fragment of around 20 cM in the O. sativa genetic background. As a preliminary step, we are now developing a genetic linkage map based on STS and RAPD markers, using an interspecific backcross between O. sativa and O. glaberrima. BC1 plants will then be selected to expedite the production of ‘contig lines’ exhibiting only the target fragment. The interspecific polymorphism is anticipated to be large enough to make this methodology feasible. Many advantages are expected from this approach, such as: (i) systematic assessment of the useful genetic resources displayed by O. glaberrima, (ii) improved and simplified detection of QTLs and (iii) comparative analysis of genomic organisation of O. glaberrima vs. O. sativa. Current work on evaluation of STS and RAPD polymorphism between parents and development of the BC1 population is presented.
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  • 45
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; aluminium-resistance ; selection ; stress ; in vitro ; hydroponic cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A selection procedure is described that isolated Al-resistant individuals from a sensitive rice (Oryza sativa L.) genotype. Somaclonal variation was used as the only source of variability and selection pressures were applied at both callus and regenerated plant levels. When 10 week-old, embryo-derived calli were submitted to different Al stresses for a period of 20 weeks. After this in vitro stress period, selected calli were grown during 18 weeks, without selection pressure, on regeneration media. Selection pressures were applied on regenerated plants (R0), and 9 R0 plants which produced seeds were selected. The transmission of the Al-resistant character to the R1, R2, R3 and R4 generations was then investigated, and three plant lines which had an increased percentage of Al-resistant plants till the fourth generation of self-pollination, were identified. One was from a callus maintained on an Al-free medium. The efficiency of in vitro selection pressures was therefore debated.
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  • 46
    ISSN: 1573-5060
    Keywords: Oryza sativa ; japonica rice cultivar ; mature embryo ; primary calli ; protoplasts ; green plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A plant regeneration system from rice protoplasts using calli derived from mature embryos was established for the two Brazilian modern rice cultivars IAC-201 and IAC-165. After 30 to 40 days of in vitro culture it was possible to obtain on average 6 million protoplasts per gram of callus. Microscopic selection of embryogenic calli was a key step for protoplast isolation. The production of embryogenic calli increased when L-proline and casein hydrolysate were used in the callus induction medium. The Oc or IR52 nurse cell lines were essential for protoplast division. Different regeneration media were studied and 139 plants were regenerated which set seed. Some of the regenerated plants showed morphological variation such as the presence of awns in spite of the short time of the in vitro culture.
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  • 47
    ISSN: 1573-5060
    Keywords: Oryza sativa ; photoperiod sensitive genic male-sterile rice (PSGMR) ; inheritance ; anther culture ; pollen plant ; polyploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The fertility segregations of F1, F2, BCF1 descended from crosses between PSGMR and japonica varieties, and F1's anther cultured homozygous diploid pollen plant populations (H2) were studied to reveal the genetic mechanism of photoperiod sensitive genic male sterility in PSGMR under natural daylight length at Shanghai. Rate of bagged seed-setting was used as an indicator of fertility. Fifteen F1 showed complete fertility similar to their parents. The ratio of completely sterile plants to fertile plants in fifteen F2 and four BCF1 was 1:15 and 1:3, respectively. The ratio of completely sterile to fertile diploid pollen plants in nine diploid populations (H2) was 1:3. These results demonstrated that the photoperiod sensitive genic male sterility in PSGMR was governed by two pairs of independent major recessive genes. There were no significant fertility segregations in hybrids F1 and selfed F2 between Nongken 58S and its derivatives 7001S, 5088S, 5047S and M105-9S, indicating that the photoperiod sensitive genic male-sterile genes in Nongken 58S were allelic to those in its derivatives. Several photoperiod sensitive genic male-sterile diploid pollen lines were bred from anther cultured homozygous diploid populations (H2) in about a three-year period. Most of these diploid lines showed significant fertility transformation and stable complete sterility from 5 August to 5 September, excellent agronomic traits and high resistance to blast and bacterial leaf blight.
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  • 48
    ISSN: 1573-5060
    Keywords: hybrid sterility ; anther indehiscence ; spikelet sterility ; pollen grain fertility ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hybrid sterility of the cross between Silewah, an Indonesian native variety and Hayakogane, a Hokkaido rice variety in Japan was confirmed to be caused by anther indehiscence, based on a lot of spikelets with fewer numbers than 3 of dehiscent anthers and fewer numbers of pollens poured onto stigma per spikelet in Silewah/Hayakogane F1. In Silewah/Hayakogane//Hayakogane BC1F1, spikelet fertility was correlated with mean number of dehiscent anthers per spikelet. So, genic analyses for hybrid sterility by anther indehiscence were conducted by scoring spikelet sterility in F2, BC1F1s and triple cross. As a result, it was concluded that the hybrid sterility was controlled by complementary action of three dominant genes. Silewah has one of the three genes and Hayakogane has the other two. It was presumed that the two genes which Hayakogane possesses were derived from those of Aikoku, an old native variety in Japan.
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  • 49
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    Plant growth regulation 21 (1997), S. 1-6 
    ISSN: 1573-5087
    Keywords: ammonium ; cell growth ; medium pH ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The possible involvements of a decrease in medium pH and accumulation of ammonium in ammonium-inhibited growth of rice cells were investigated. Ammonium, applied at concentrations ranging from 20 to 50 mM, markedly inhibited cell growth and decreased medium pH. The accumulation of ammonium in rice cells was observed only when ammonium concentration was 40 mM or higher. Ammonium-inhibited growth was alleviated when medium pH was buffered with MES [2-(N-morpholino)-ethanesulfonic acid]. However, no difference in ammonium level was observed between buffered and unbuffered ammonium-fed rice cells. Succinic acid, α-ketoglutaric acid, glutamic acid and glutamine were found to be effective in reversing ammonium-inhibited growth of rice cells and reducing a fall in pH in the ammonium-fed medium. Succinic acid, α-ketoglutaric acid and glutamic acid decreased the level of ammonium in ammonium-fed rice cells. However, glutamine was unable to decrease the ammonium level in ammonium-fed rice cells. The current results suggest that a decrease in medium pH is a factor responsible for growth inhibition of ammonium-fed rice cells.
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  • 50
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    Plant growth regulation 22 (1997), S. 163-168 
    ISSN: 1573-5087
    Keywords: Oryza sativa ; paraquat ; putrescine ; spermidine ; spermine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The protective effect of polyamines against paraquat (PQ) toxicity of rice (Oryza sativa) leaves was investigated. PQ treatment resulted in a higher putrescine (PUT) and lower spermidine (SPD) and spermine (SPM) levels in rice leaves. Pretreatment with SPD and SPM, which resulted in a 10- and 20-fold increase in endogenous level of SPD and SPM, respectively, reduced PQ toxicity (30%). Limited reduction of PQ toxicity by exogenous SPD and SPM is most likely due to the fact that they are not readily transported in rice leaf cells and localized to those areas along the cut edges of detached rice leaves [4]. PUT pretreatment did not increase endogenous SPD and SPM levels and had no effect on reducing PQ toxicity. It was found that 1,10-phenanthroline, an iron chelator, treatment reduced the toxicity of PQ (35%) and increased the levels of SPD (27%). The results indicate that reduction of PQ toxicity by SPD and SPM is due to increased activities of catalase (18%) and peroxidase (40%).
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  • 51
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    New forests 14 (1997), S. 33-44 
    ISSN: 1573-5095
    Keywords: nutrient concetration ; frost hardiness ; growth cessation ; Pinus sylvestris ; visual damage ; nitrogen ; phosphorus ; potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In this study the effect of summer fertilization on the initiation of frost hardening of containerized second-year Scots pine (Pinus sylvestris L.) seedlings is studied. During the second growing season three different fertilization programs (water soluble NPK with micronutrients) determined by electrical conductivity of peat water extract (0.2, 0.5 and 1.2 mS cm-1) were initiated. The growth and nutrient concentrations of needles were monitored during the fertilization period. The frost hardiness of seedlings was assessed on four separate occasions at two week intervals from August 7 to September 18. This assessment was based on artificial freezing tests and visual damage scoring of tissue browning on current-year needles. Clear differences in foliar N, P and K concentrations were observed between the fertilization treatments. Fertilization prolonged the growing period of needles and increased root collar diameter. In all the tests, the highest fertilization level resulted in the highest level of frost hardiness. The difference between the fertilization treatments ranged from 1 °C to 2.2 °C. Frost hardiness increased with an increase in foliar nitrogen concentration and slightly less consistently with increases in foliar phosphorus and potassium concentrations.
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  • 52
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    Plant growth regulation 21 (1997), S. 37-42 
    ISSN: 1573-5087
    Keywords: abscisic acid ; growth ; methyl jasmonate ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of abscisic acid (ABA) and methyl jasmonate (MJ) on growth of rice seedlings were compared. The lowest tested concentration of ABA and MJ that inhibited seedling growth was found to be 4.5 and 0.9 µM, respectively. Growth inhibition by ABA is reversible, whereas that by MJ is irreversible. GA3 was found to be more effective in reversing inhibition of shoot growth by ABA than by MJ. KCl partially relieved MJ-inhibited, but not ABA-inhibited, growth of rice seedlings. The beneficial effect of K+ on growth of rice seedlings in MJ medium could not be replaced by Li+, Na+ or Cs+. MJ treatment caused a marked release of K+ into the medium. In order to understand whether cell wall-bound peroxidase activity was inversely related to rice seedling growth, effects of ABA and MJ on cell wall-bound peroxidase activity were also examined. Results indicated that both ABA and MJ increased cell wall-bound peroxidase activity in roots and shoots of rice seedlings. Although MJ (4.5 µM) was less effective in inhibiting root growth than ABA (9 µM), MJ was found to increase more cell wall-bound peroxidase activity in roots than ABA.
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  • 53
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    Plant growth regulation 21 (1997), S. 195-201 
    ISSN: 1573-5087
    Keywords: abscisic acid ; ammonium ; benzyladenine ; leaf senescence ; methionine sulfoximine ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The relationship between ammonium accumulation and senescence of detached rice leaves was investigated. Ammonium accumulation in detached rice leaves coincided closely with dark-induced senescence. Exogenous NH4Cl and methionine sulfoximine, which caused an accumulation of ammonium in detached rice leaves, promoted senescence. Treatments such as light and benzyladenine, which retarded senescence, decreased ammonium level in detached rice leaves. Abscisic acid, which promoted senescence, increased ammonium level in detached rice leaves. The current results suggest that ammonium accumulation may be involved in regulating senescence. Evidence was presented to show that ammonium accumulated in detached rice leaves increases tissue sensitivity to ethylene. The accumulation of ammonium in detached rice leaves during dark-induced senescence is attributed to a decrease in glutamine synthetase activity and an increase in reduction of nitrate.
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  • 54
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    Biogeochemistry 36 (1997), S. 189-203 
    ISSN: 1573-515X
    Keywords: ammonia volatilization ; grassland ; nitrogen ; ungulate ; Yellowstone National Park
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract We measured ammonia volatilization at three topographic positions(hilltop, mid-slope, slope-bottom) on three grassland landscapes at threetimes during 1995 (April, May, July) on the northern winter range ofYellowstone National Park that supports large herds of native ungulates.Percent ammonia-N lost from all sites during the study ranged 1–24%of urea-N applied. Volatilization among sites was negatively related tosoil cation-exchange capacity (r = –0.85) and rates were highest inJuly. We used the relationship between soil CEC and percent Nvolatilized from urea-amended plots to estimate annual ammonia-Nvolatilization from 5 sites for which annual ungulate urine inputs werepreviously determined (Frank et al. 1994). Estimated mean annualammonia-N volatilized from those sites was 1.4 kg/ha/yr, which wasless than a previously reported regional atmospheric deposition rate (2kg/ha/yr; Swank 1984). Results indicate the need to understand theinteraction between (1) spatially heterogeneous patterns of soilprocesses, and 2) nonuniform patterns of ungulate use of landscapes todetermine rates of ecosystem-level N-gaseous loss. Findings alsosuggest that ammonia-N volatilized from urine patches should not leadto a decline in soil N in this ecosystem.
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  • 55
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    Biogeochemistry 37 (1997), S. 237-252 
    ISSN: 1573-515X
    Keywords: freshwater ; limitation ; marine ; nitrogen ; phosphorus ; ratio ; stoichiometry ; trace elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Nitrogen supply is often assumed to limitmarine primary production. A global analysis of totalnitrogen (N) to phosphorus (P) molar ratios shows thattotal N:P is low (〈16:1) in some estuarine andcoastal ecosystems, but up to 100:1 in open oceans.This implies that elements other than N may limitmarine production, except in human impacted, estuarineor coastal ecosystems. This pattern may reconcileconflicting enrichment studies, because N additionfrequently increases phytoplankton growth where totalN:P is expected to be low, but P, Fe, or Si augmentphytoplankton growth in waters where total N:P ishigh. Comparison of total N:P stoichiometry betweenmarine and freshwaters yields a model of the form ofthe aquatic N:P cycle.
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  • 56
    ISSN: 1573-515X
    Keywords: carbon ; nitrogen ; Ohio River ; phosphorus ; Red field ratios ; dissolved organic matter ; rivers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract A 12-month study was conducted to measure the concentrations ofdissolved organic matter (DOC, TDN, TDP) in four sites within a119 km long reach of the Ohio River, near Louisville, KY. In thisstudy we test whether specific geomorphological and biologicalfactors influenced variations in dissolved organic matter.Concentrations of DOC in the river averaged ≈1200μmol/L, and varied by nearly two orders of magnitudeseasonally (mean DOC during base flow ≈620 μmol/L).Peak periods for DOC at all sites were during April–May. Thesite nearest a navigation dam (deeper, lower current velocities)had significantly lower concentrations of TDN and greater C:Nratios than upstream sites. The largest tributary entering thisreach (Kentucky River) had no significant effect on levels of DOMin the main river, despite having significantly greaterconcentrations of TDN and lower levels of DOC during most monthsof the year. Concentrations of DOC, TDN, and TDP were notsignificantly different in littoral and pelagic habitats at allsites studied, suggesting little floodplain influence on DOM inthis constricted-channel section of the Ohio River. C:N ratios ofDOM in the Ohio were significantly different among seasons; C:Nexceeded or equaled Redfield ratios in summer and fall (6 to 10),but were below Redfield (1.8 to 3.0) during winter and spring.Regression models suggest that total phytoplankton densities andflow conditions are the two most important factors regulating DOMin this very large river.
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  • 57
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    Plant molecular biology 35 (1997), S. 197-203 
    ISSN: 1573-5028
    Keywords: genetic engineering ; particle bombardment ; plant biotechnology ; transgenic rice ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bombardment-based methodology is responsible for the effective genetic manipulation of major cereals including rice. Many groups reported significant advances on various aspects of rice molecular biology and genetic engineering using procedures based on bombardment technology. Molecular and genetic characterization of large numbers of these plants (more than 500 independent transgenic plants) provided information on structure, expression and stability of integrated DNA through multiple generations. Such evaluations were carried out in the greenhouse and in the field. Stability of expression was found to be dependent on the nature of the promoter and the transgene, and in specific cases on gene copy number. Direct DNA transfer utilizing particle bombardment for the delivery of foreign DNA into rice tissue results in the recovery of large numbers of independently derived transgenic plants in a variety-independent fashion. Gene copy number, level and stability of expression of transgenes can be compared to other DNA delivery methods, direct or indirect, including Agrobacterium-mediated gene transfer. In this paper, the technology is summarized and discussed in terms of present and future applications, including field trials and potential commercialization of transgenic rice expressing a number of genes of agronomic interest such as pest and herbicide resistance.
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  • 58
    ISSN: 1573-5044
    Keywords: genotype ; in vitro culture ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The regeneration response of mature and immature rice embryos from 15 rice varieties (Oryza sativa L. var. indica) was separately measured in terms of regeneration per cent and total number of regenerated plantlets obtained for a fixed sample size per variety, when cultured on standard MS media. The response of the rice varieties was classed as high, medium or poor, based on the above criteria. The regeneration per cent of genotypes showing high, medium and poor response ranged from 67 to 97, 38 to 63 and 0 to 36 respectively. The variation in the regeneration response amongst the defined groups was found to be statistically significant in terms of regeneration per cent and total plantlets for a constant sample size when subjected to one way analysis of variance and Duncan's multiple range test. Only small influences on regeneration frequency were found when the most and least responsive genotypes, Binnatoa and BR-23 were cultured on N6 and MS media with additional or different supplements.
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  • 59
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    Journal of chemical ecology 23 (1997), S. 979-993 
    ISSN: 1573-1561
    Keywords: Terpenes ; α-pinene ; camphor ; borneol ; cineole ; emission ; carbon-based secondary compounds ; biomass ; nitrogen ; water ; carbon dioxide concentrations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Rosmarinus officinalis L. plants were grown under carbon dioxide concentrations of 350 and 700 μmol/mol (atmospheric CO2 and elevated CO2) and under two levels of irrigation (high water and low water) from October 1, 1994 to May 31, 1996. Elevated CO2 led to increasingly larger monthly growth rates than the atmospheric CO2 treatments. The increase was 9.5% in spring 1995, 23% in summer 1995, and 53% in spring 1996 in the high-water treatments, whereas in low-water treatments the growth response to elevated CO2 was constrained until the second year spring, when there was a 47% increase. The terpene concentration was slightly larger in the elevated CO2 treatments than in atmospheric CO2 treatments and reached a maximum 37% difference in spring 1996. There was no significant effect of water treatment, likely as a result of a mild low water treatment for a Mediterranean plant. Terpene concentration increased throughout the period of study, indicating possible age effects. The most abundant terpenes were α-pinene, cineole, camphor, borneol, and verbenone, which represented about 75% of the total. No significant differences were found in the terpene composition of the plants in the different treatments or seasons. The emission of volatile terpenes was much larger in spring (about 75 μg/dry wt/hr) than in autumn (about 10 μg/dry wt/hr), partly because of higher temperature and partly because of seasonal effect, but no significant difference was found because of CO2 or water treatment. The main terpene emitted was α-pinene, which represented about 50% of the total. There was no clear correlation between content and emission, either quantitatively or qualitatively. More volatile terpenes were proportionally more important in the total emission than in total content and in autumn than in spring.
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    World journal of microbiology and biotechnology 14 (1997), S. 113-118 
    ISSN: 1573-0972
    Keywords: Aspergillus ; continuous culture ; glucoamylase ; growth ; fungi ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Maltose-limited continuous culture of Aspergillus niger was carried out with potassium nitrate to investigate growth and glucoamylase formation characteristics. Glucoamylase production was dependent on the specific growth rate. The maximal amount of glucoamylase (units/l and U/g dry weight) was obtained at μ=0.08h−1, and the maximum specific rate of production (units/g/dry weight per hour) was at μ=0.2h−1. The maintenance coefficients (ms and mATP) were higher than for some other fungi. Maximal growth yields on substrate, oxygen and ATP (Yxsm, YxO2m and Yxam) were very efficient (high) and the value of Yxam, which cannot exceed the theoretical maximal value, is obtained when a P/O ratio of 1:1 is assumed. This indicates that biomass formation is energetically inexpensive and most of the expended energy has to be invested in the process of glucoamylase excretion.
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    Agroforestry systems 38 (1997), S. 51-76 
    ISSN: 1572-9680
    Keywords: nitrogen ; nutrient cycling ; phosphorus ; soil fertility ; soil organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Trees can influence both the supply and availability of nutrients in the soil. Trees increase the supply of nutrients within the rooting zone of crops through (1) input of N by biological N2 fixation, (2) retrieval of nutrients from below the rooting zone of crops and (3) reduction in nutrient losses from processes such as leaching and erosion. Trees can increase the availability of nutrients through increased release of nutrients from soil organic matter (SOM) and recycled organic residues. Roots of trees frequently extend beyond the rooting depth of crops. Research on a Kandiudalfic Eutrudox in western Kenya showed that fast-growing trees with high N demand (Calliandra calothyrsus, Sesbania sesban and Eucalyptus grandis) took up subsoil nitrate that had accumulated below the rooting depth of annual crops. Sesbania sesban was also more effective than a natural grass fallow in extracting subsoil water, suggesting less leaching loss of nutrients under S. sesban than under natural uncultivated fallows. Nutrient release from SOM is normally more dependent on the portion of the SOM in biologically active fractions than on total quantity of SOM. Trees can increase inorganic soil N, N mineralization and amount of N in light fraction SOM. Among six tree fallows of 2- and 3-year duration on an Ustic Rhodustalf in Zambia, inorganic N and N mineralization were higher for the two tree species with lowest (lignin + polyphenol)-to-N ratio (mean = 11) in leaf litter than for the two tree species with highest ratio (mean = 20) in leaf litter. Trees can also restore soil fauna, which are important for SOM and plant residue decomposition. Some agroforestry trees have potential to provide N in quantities sufficient to support moderate crop yields through (i) N inputs from biological N2 fixation and retrieval of nitrate from deep soil layers and (ii) cycling of N from plant residues and manures. The cycling of P from organic materials is normally insufficient to meet the P requirements of crops. Sustained crop production with agroforestry on P-deficient soils will typically require external P inputs.
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  • 62
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    Plant and soil 196 (1997), S. 321-325 
    ISSN: 1573-5036
    Keywords: lettuce ; nitrogen ; phosphorous ; potassium ; nutrient limitation ; nutrient remobilisation ; relative growth rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The relationship between plant nutrient concentration and relative growth rate (RGR) was studied under non-steady state conditions using data from a new N interruption experiment with young lettuce plants grown hydroponically in the glasshouse. RGRs estimated from the fit of a versatile growth model were shown to decline curvilinearly with plant N concentration as N deficiency increased. Similar curvilinear relationships were also derived when the same model was used to reanalyse data for N, P and K interruption treatments from other experiments previously published in the literature. These results clearly indicate that the rate of remobilisation of nutrient reserves varies with the nutrient status of the plant. This contrasts with the linear relationships observed where the changes in plant N concentration occurred solely as a response to increasing plant age, or when plants were grown under steady state conditions with constant relative nutrient addition rates. These differences in the pattern of response provide strong evidence to support the hypothesis that the form of the relationship between RGR and plant nutrient concentration can vary depending upon whether a plant's external supplies or internal reserves of a particular nutrient are more limiting.
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  • 63
    ISSN: 1573-5036
    Keywords: Allium ursinum ; acidification ; beech stand ; nitrogen ; nutrient leaching ; soil mesofauna ; soil solution chemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aboveground biomass built up annually by Allium ursinum (L.) contains similar amounts of nutrients as the foliage of mature Fagus sylvatica (L.) stands. The decomposition of the A. ursinum stand in early summer provides N rich forage for grazing mesofauna, especially favouring collembolans and accelerating mineralization of soil organic matter. Short term decreases of soil pH had no negative effect on populations of collembolans. Synergistic effects from soil fauna and microbes may accelerate nitrogen release from decomposing leaf litter. A positive feed back may have emerged. High animal abundance and diverse mesofauna populations are capable of high rates of litter fragmentation. Consequently, favourable conditions for microorganisms are created and allow high rates of mineralization and release of nutrients. Our data show that substantial amounts of nitrogen are lost from the system. Undisturbed forest ecosystems are considered to recycle mineralized nitrogen efficiently though. But temporal uncoupling of the N cycle due to microbial activity and delayed or decreased N uptake of higher plants can cause enhanced leaching even from undisturbed systems. The tendency to loose nutrients is apparent from high nitrate concentrations in the soil solution throughout the year. When nutrient losses from A. ursinum subsystems are considered, lateral nutrient imports from adjacent parts of the ecosystem have to be taken into account. If lateral import does not counterbalance losses, maintenance of the soil nutrient status must occur by weathering or a decline is to be expected.
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  • 64
    ISSN: 1573-5036
    Keywords: cowpea ; crop residues ; mineralisation ; nitrogen ; phosphorusconcentration ; t Stylosanthes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Laboratory studies were conducted to investigate the effect of phosphorus concentration in residues of cowpea (t Vigna unguiculata, L. Walp) and stylo (t Stylosanthes hamata, L., cv Verano) on their rate of nitrogen mineralisation when incubated in a soil whose P status was deficient for plant growth. Residues with a range of P concentrations were obtained by applying varying rates of P to soil in which the plants were grown in the field or the glasshouse. Variations in P concentration of field- or glasshouse-grown residues were not accompanied by variations in other chemical components (C:N ratio, lignin and polyphenol concentrations). Both lignin and polyphenol concentrations were higher in the field-grown than in the glasshouse-grown residues. Lignin concentration was greater in cowpea than in stylo, but polyphenols were higher in stylo. Cowpea residues mineralised N less rapidly than stylo. N mineralisation from residues with low P concentration was consistently less than from those of higher P concentration; reduced mineralisation was observed for P concentration in the residues below 1.6 g kg−1. When inorganic P was added to the residue-soil systems, N mineralisation from the residues was increased, though no interaction between the effects of adding inorganic P and P concentration in the residues was observed.
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  • 65
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    Plant and soil 192 (1997), S. 237-247 
    ISSN: 1573-5036
    Keywords: immobilization ; mineralization ; nitrogen ; residues ; sugarbeet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The leaves and crowns from 15N-labelled sugar beets were incubated in either a silty clay loam or sand soil for almost one year. Four additions of fresh, chopped residues mixed with soil were tested: 15N-labelled leaves alone, 15N-labelled leaves plus unlabelled crowns, unlabelled leaves plus 15N-labelled crowns, and 15N-labelled crowns alone; a control with no addition was also incubated. The C:N ratio of the leaves was 11 and that of the crowns 40. Incubations were carried out in pots kept at 20 °C and optimal moisture conditions. The leaves mineralized N from the start of the experiment but the addition of crowns to soil at first caused immobilization of nitrogen followed eventually by mineralization after 6 or 12 weeks depending on soil type. The extra amounts of mineral N found in soil at the end of the experiment where additions were made corresponded to the sum of the background mineralization and the addition; no priming effects were encountered. Very slight differences only were found between the initial rates of mineralization of C in all of the treatments. Although there was also little difference between the sand and silty clay loam soils in the direct mineralization of nitrogen from the sugar beet leaves, where N was first immobilized (i.e. from crowns or a mixture) re-release of N took place more quickly in the sand soil. The total recovery of15 N found in soils after 24 weeks incubation ranged from 70% to 90% with least being lost from the sugar-rich but N-deficient crowns. Where leaves plus crowns were incubated together both residues contributed to the microbial biomass N. In practice, immobilization of this magnitude and duration (expressed as a temperature sum) could exceed the growth period of a spring sown crop. The actual immobilization found in any one field is likely to depend on the C:N ratio of the residues and could account for much of the variation in the residual benefit of sugar beet residues reported in the literature.
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  • 66
    ISSN: 1573-5036
    Keywords: catch crop ; intensive dairy farming ; maize silage ; mineral nitrogen ; nitrogen ; slurry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrate leaching from a double-cropping forage system was measured over a 2-year period (June 1994–May 1996) in the Northwest region of Portugal using ceramic cup samplers. The crops were grown for silage making and include maize (from May to September) and a winter crop (rest of the year) consisting of a mixture of cereals and Italian ryegrass. The experiment was performed on two different sites with a history of many years under the same crop and fertiliser management, but differing in the amounts of N applied as fertiliser and by regular cattle slurry applications. The annual nitrate leaching losses measured ranged from 154 to 338 kg N ha-1. These amounts lead to annual mean concentrations between 22 and 41 mg $$NO_3^ - $$ -N L-1 in the drained water. The coarse textured soils (sandy loams) and the climatic conditions of the region with more than 600 mm of drainage concentrated between October and March, tended to promote the leaching of all the nitrate-N left in the soil after the maize crop plus the N released by mineralization during the winter period. On these soils, the minimum amount of drainage (necessary to provide the complete leaching of all the nitrate-N in the soil profile in the end of summer), seems to be between 300 and 400 mm. The winter crops removed important quantities of N (83–116 kg N ha-1) but, due to their late establishment in autumn they did not succeed in taking up the nitrate-N left in the soil after the maize crop. Approaches for reducing the nitrate leaching losses in this particular system are discussed.
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  • 67
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    Euphytica 95 (1997), S. 253-258 
    ISSN: 1573-5060
    Keywords: rice ; Oryza sativa ; widely compatible restorer ; fertility restoration ability ; anther culture ; inheritance ; Indica-japonica heterosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Following anther culture of various F1 hybrids of indica restorer (R) lines/wide compatible varieties (WCVs) and japonica R lines/WCVs, the homozygous diploid plants (2n) generated were test-crossed with indica WA type cytoplasmic-genetic male sterile (CMS) line Zhanshan 97A (WA), indica testers Nanjing 11 and Nante, japonica BT CMS line Hanfeng A (BT), and japonica testers Balilla and Akihikari to identify widely compatible restorer lines. The results of this study showed that among the diploid pollen plants generated from F1 hybrids of indica R lines/WCVs, 36.7% and 64.7% possessed normal fertility restoration ability (rate of seed-setting 〉 80%) to Hanfeng A and Zhanshan 97A, respectively. 71.3% and 32.3% had normal fertility restoration ability to Hanfeng A and Zhanshan 97A, respectively, in diploid pollen plants derived from the japonica R lines/WCVs F1's anther culture. Several widely compatible R lines were selected from anther culture of F1 hybrids of indica R lines/WCVs and japonica R lines/WCVs. These widely compatible R lines derived from diploid pollen plants showed good wide compatibility and restoration ability both to WA and BT type CMS lines. Strong standard heterosis of major agronomic traits and yield traits was observed in F1 test-crosses of widely compatible R lines with Hanfeng A and Zhanshan 97A. The wide compatibility in widely compatible R lines H17, H158 and H281 was studied by a set of three-way crosses. Segregation of the fertile plants with seed-setting rate 〉 70% and semi-fertile plants with seed-setting rate 〉 69.9% agreed with a ratio of 1:1, indicating that wide compatibility in these widely compatible R lines is governed by a pair of major genes.
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  • 68
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    Euphytica 95 (1997), S. 301-307 
    ISSN: 1573-5060
    Keywords: gene location ; heading time ; Oryza sativa ; photoperiod sensitivity ; rice ; trisomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Recent genetic analyses on heading-time of rice indicated that almost all the well-adapted varieties in the temperate zone carry a strong photoperiod-sensitivity gene E1, a dominant allele of E1 locus. In order to identify the chromosome on which E1 is located, a trisomic analysis was made using two primary trisomic series originating from the japonica varieties, Nipponbare and Kinmaze, respectively. The Nipponbare and Kinmaze series were crossed with heading-time tester lines, EG0 and EG3, respectively, both of which did not carry the E1. The F2 populations for chromosome 1, 2, and 3 could not be analyzed due to lack of seed. All the other F2 populations showed distinct segregation into early-type and late-type plants caused by the E1 locus segregation, which suggested that the trisomic analysis for E1 locus could be efficiently made. Both disomic and trisomic groups in the F2 population from the cross of the trisomic line for chromosome 7 × EG0 showed a segregation ratio significantly different at the 1% level from a ratio of 1 [e1e1; early]: 3 [E1e1, E1E1; late]. This suggested that E1 was located on chromosome 7. Subsequently, the linkage analysis was made using three morphological marker genes on chromosome 7. It was recognized that E1 was linked to rfs (rolled fine strip gene) and slg (slender glume gene) with recombination values of 16.3 ± 5.88 and 9.1 ± 4.72%, respectively. From these results, it can be concluded that E1 is most likely to be located on chromosome 7.
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  • 69
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    Euphytica 96 (1997), S. 135-142 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; chilling tolerance ; in vitro selection ; rice ; somaclonal variation ; field performance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Embryo-derived calli of four rice varieties cultivated at high altitude in Burundi — Facagro 57, Facagro 76, Kirundo 3 and Kirundo 9 — were submitted to different temperature regimes. The percentage of regenerating calli greatly varied depending on variety, length of culture and callus temperature treatment. The reduction of regeneration percentages induced by low temperature was more pronounced in the more sensitive varieties. Regenerated plants (R0) and their progenies in R1, R2 and R3 were cold-screened together with control plants. In all varieties, significantly higher survival rates were obtained in R3 with in vitro plants than with control plants. Such chilling tolerance improvement was not obtained following a massal selection applied during 3 successive generations onto the control plants. In vitro plants regenerated from calli cultivated either at 25 °C, either at 4 °C, were cultivated at different altitudes in Burundi during two successive generations. For most observed traits, the in vitro plants were characterized by lower means, larger variation and higher maximum values than the control plants. The most chilling-tolerant somaclonal families were most usually characterized by extensive differences in fatty acid composition, chilling-induced electrolyte leakage and chlorophyll fluorescence, compared to the varieties they derived from.
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  • 70
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    Euphytica 97 (1997), S. 25-29 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; amylograph viscosity characteristics ; inheritance ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Southern United States long grain rice (Oryza sativa L.) is a unique market type characterized by intermediate amylose content, gelatinization temperature, and amylograph viscosity profile characteristics. Rice breeders sometimes use long grain germplasm with differing amylograph viscosity profiles. The inheritance of rice amylograph viscosity profiles has not been ascertained and, thus, was the objective of this study. A six parent diallel (parents plus F1 hybrids) was evaluated at Stuttgart, AR during 1994 and 1995. In 1994, 65 F2 plants from the ‘L-202’ × ‘Newrex’ and L-202 × ‘Katy’ crosses were space planted, and in 1995, the 65 F2:3 lines were planted as hill plots. The inheritance of amylograph viscosity profiles appeared to be controlled by a single locus with genes with additive effects. The results of the diallel analysis also support control by a single locus with genes with additive effects because GCA effects were significant and SCA effects nonsignificant for peak, hot paste, and cool paste viscosities. Intermediate amylose content and intermediate amylograph viscosity profiles were strongly correlated. Selection first for intermediate amylose content followed by reselection for intermediate hot paste viscosities would identify long grain genotypes with southern United States long grain cooking quality in progeny from crosses between southern United States long grain rice genotypes and softer cooking long grain genotypes.
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  • 71
    ISSN: 1573-5060
    Keywords: isozyme ; differentiation ; Oryza sativa ; rice ; upland rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two rice ecotypes, the so-called lowland and upland populations, which carry different isozyme genotypes mostly at a single locus, are cultivated in Japan. The aim of this study was to examine the origin and the mechanism for keeping these genetic differences. The upland population is cultivated in upland fields and carries a different allele for a particular isozyme gene, Pgd-1, which has never been found in the lowland population. RFLP markers showed a weak trend for genetic differentiation between the two ecotypes. On the other hand, morphological, and physiological traits showed marked differences between the two ecotypes. Furthermore, based on the genotypic difference, two Japonica subgroups are defined in the upland population. Subgroup I is the minor group and carries key lowland characters, including the genotype for PGD. Subgroup II carries different traits and the genotype for PGD of the alternative subgroup. As an allelic difference for Pgd-1 is known to occur between the two ecospecies, Tropical (Tr) and Temperate (Tm) Japonicas, upland cultivars can be classified by diagnostic characters which distinguish a variety into Tr or Tm type. The upland population consists of three types of cultivars, Tr-, Tm- and intermediate-type. In contrast, the lowland population consists of a uniform Tm type Japonicas. As Japanese upland cultivars still have an isozyme allele specific to the Tr type, the upland population has a rather complex constitution which is presumably now being introgressed by lowland genetic material, but still represents a major difference at some genetic levels. Upland rice carries several stress-resistant genes which would be useful for lowland rice breeding. The genetic difference would be efficient for tagging upland specific traits by upland specific genetic markers.
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  • 72
    ISSN: 1573-5060
    Keywords: fertility restoration ; hybrid rice ; linkage analysis ; Oryza sativa ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hybrid varieties developed by making use of the wild abortive cytoplasmic male sterility system account for 90% of hybrid rice produced. Previous inheritance studies have established that the fertility restoration in this system is controlled by two major loci, but the chromosomal locations of the fertility restorer (Rf) loci have yet to be resolved. In this study we determined the genomic locations of the two Rf loci by their linkage to molecular markers. The Rf gene containing regions were identified by surveying two bulks, made of 30 highly fertile and 46 highly sterile plants from a large F2 population of the cross between Zhenshan 97A and Minghui 63, with RFLP markers covering the entire rice genome. The survey identified two likely Rf gene containing regions, located on chromosomes 1 and 10 respectively. This was confirmed by ANOVA using a large random sample from the same F2 population and also with a genome-wide QTL analysis of a test-cross population. The results also showed that both loci have major effects of almost complete dominance on fertility restoration and the effect of the locus on chromosome 10 is larger than the one on chromosome 1. The two loci acted as a pair of classical duplicate genes; a single dominant allele at one of the two loci would suffice to restore the fertility to normal or nearly normal. Closely linked markers identified in this study may be used for marker assisted selection in hybrid rice breeding programs.
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  • 73
    ISSN: 1573-5060
    Keywords: Oryza sativa ; Aus ; rice ; hybrid sterility ; isozomes ; varietal diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Magnitudes of genetic variation within each of major varietal groups of cultivated rice were surveyed in terms of isozyme polymorphism and allelic differentiation of hybrid sterility loci, both of which are considered to have litt le selective value. Allelic differences for 20 isozyme loci were examined in a total of 337 accessions, including Indica and Japonica rices. Aus cultivars from India, Javanica cultivars and both landraces and cultivars from China. Eleven out of the 20 isozyme loci were polymorphic. The Aus cultivars contained more alleles per locus than others. The hybrid sterilities in the crosses among Chinese cultivars, Indica and Japonica cultivars were mainly controlled by locus S-5, whereas the hybrid sterilities of Aus cultivars, when they were crossed to Indica, Japonica or Javanica cultivars, were found to be controlled by allelic interaction at hybrid sterility loci S-5, S-7, S-9 and S-15. Also in terms of the number of alleles at S-5 and S-7, Aus cultivars contained more alleles than others. While the Aus group showed an extreme diversity for both hybrid sterility alleles and isozymes, modern cultivars from the south of YangZi River in China were classified into Indica type and those from the north were into Japonica, which were almost the same as those in Japan. On the basis of the measured polymorphism the Indica-Japonica differentiation was explained by founder effects, i.e., through selection and distribution of two original sources each with a unique set of markers. Contrastingly, the continuous and pronounced diversity in the Aus group was attributed to the absence of such a process. The intermediate groups in Yunnan province and Tai-hu Lake region of China are considered to be isolated from such founder effects, retaining an intermediate diversity in terms of isozymes and hybrid sterility genes. Since hybrid sterility hampers the exchange of genes between cultivars of different groups, the understanding of its genetic basis will be important in rice breeding, particularly in hybrid rice breeding.
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  • 74
    ISSN: 1573-5060
    Keywords: Oryza rufipogon ; Griff ; Oryza sativa ; interspecific hybrid ; hybrid swarms ; cytological abnormalities ; evolution and rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The morphology and cytology of selected hybrid swarms of wild and cultivated rices were studied following natural hybridization between the native species Oryza rufipogon Griff and exotic species Oryza sativa L. (var. Thaothabi and Moirangphoe). Similar studies were also conducted on populations developed through artificial hybridization of those two species. The morphological variation in plant height, anthocyanin pigmentation, Kernel and husk colour, sterility, presence of awns and grain shattering habits were similar in both natural (hybrid swarms) and artificially derived populations of interspecific hybrids. The cytological abnormalities such as quadrivalant formation at metaphase I, delayed separation, laggards and bridge formation, unequal separation and chromosomal elimination were also observed in both populations. These results indicated that the evolution of intermediate types had occurred due to genetic introgression from cultivated species to wild species. A systematic extinction of founder population was also noticed due to sudden ecological changes like deep submergence to medium submergence and human interference by growing cultivated species O. sativa L. in the area where wild species were being grown.
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  • 75
    ISSN: 1573-5028
    Keywords: Oryza sativa ; rice ; drought stress ; abscisic acid (ABA) ; QTL analysis ; comparative genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants accumulate abscisic acid (ABA) under droughted conditions. Genetic variation in the accumulation of ABA in detached and partially dehydrated leaves of rice has previously been reported, and this was found to be associated with variation in leaf size (smaller leaves made more ABA). Correlation analysis failed to distinguish clearly between a causal relationship between the two traits and close genetic linkage between loci controlling the traits. Here we present a detailed genetic analysis of ABA accumulation in detached and partially dehydrated rice leaves, using a population of F2 plants generated from the lowland × upland cross IR20 (high-ABA) × 63-83 (low-ABA) which was mapped with RFLP and AFLP markers. Several highly significant quantitative trait loci (QTLs) for ABA accumulation and leaf weight were identified. Only one of the minor QTLs for ABA accumulation (accounting for only 4% of the phenotypic variance) was coincident with any QTLs for leaf size such that the high-ABA allele was associated with smaller leaves. This analysis, therefore, showed that the association previously found between ABA accumulation and leaf size was probably largely due to genetic linkage and not to a direct effect of leaf size on ABA accumulation or vice versa. Because of the importance of ABA accumulation in regulating responses of plants to drought stress and the effects of plant size on the rate of development of stress, QTLs for drought-induced ABA accumulation, leaf size and tiller number were compared between rice and wheat. In particular, a possible location in rice was sought for a homoeologue of the major wheat vernalization responsive gene, Vrn1, as this gene is also associated with major effects on leaf size, tiller number and ABA accumulation in wheat. The likelihood of homoeologous loci regulating ABA accumulation, leaf size and tiller number in the two crops is discussed.
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  • 76
    ISSN: 1573-515X
    Keywords: Amazon ; deforestation ; hydrologic pathway ; groundwater ; nitrogen ; rain forest ; slash-and-burn agriculture ; solutes ; tropical
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Hydrochemical changes caused by slash-and-burnagricultural practices in a small upland catchment inthe central Amazon were measured. Soluteconcentrations were analyzed in wet deposition,overland flow, shallow throughflow, groundwater andbank seepage in a forested plot (about 5 ha) and anadjacent plot (about 2 ha) which had been deforestedin July 1989 and planted to manioc, and in streamwater in partially deforested and forested catchments. Measurements were made from November 1988 to June1990. The effects of slash-and-burn agriculturalpractices observed in the experimental plot includedincreased overland flow, erosion, and large losses ofsolutes from the rooted zone. Concentrations ofNO3 -, Na+, K+, SO4 2-,Cl- and Mn in throughflow of the experimentalplot were higher than those of the control plot bymore than a factor of 10. Extensive leaching occurredafter cutting and burning, but solute transfers werediminished along pathway stages of throughflow togroundwater, and particularly within the riparian zoneof the catchment. High concentrations of N and P inoverland flow indicate the importance of usingforested riparian buffers to mitigate solute inputs toreceiving waters in tropical catchments.
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  • 77
    ISSN: 1573-5117
    Keywords: nitrogen ; phosphorus ; phytoplankton biomass ; Daphnia grazing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of nitrogen as a factor controllingphytoplankton biomass was studied in nutrientenrichment incubations in the laboratory using waterfrom pelagic region of two mesotrophic lakes ineastern Finland, Lake Kallavesi (in year 1994) andLake Juurusvesi (in year 1995). We used differentcombinations of phosphorus and nitrogen additions ina total of eight experiments. Furthermore, we includedDaphnia grazing treatment to the experimentaldesign in Lake Juurusvesi experiments. The nitrogentreatments did not increase chlorophyll aconcentration in any of the experiments compared withthe controls. Chlorophyll a content was highestin those nutrient treatments where phosphorus wasadded with or without nitrogen. Daphnia grazingdecreased chlorophyll a concentration comparedwith non-grazed treatments. In some cases grazing alsocaused higher ammonium concentrations. Theseexperiments, as well as the nutrient ratio of the lakewater used, suggest that phosphorus is likely tocontrol the amount of phytoplankton biomass.
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  • 78
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    Hydrobiologia 363 (1997), S. 117-126 
    ISSN: 1573-5117
    Keywords: nutrient limitation ; eutrophication ; nitrogen ; phosphorus ; Archipelago Sea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eutrophication is the most acute environmentalproblem in the Archipelago Sea, SW Finland. Whenanalysing the factors behind this escalatingeutrophication the determination of limitingnutrient at a given time is essential. Besidesexperimentations, nutrient limitation of planktonhas been extensively studied by direct chemicalanalyses. We used the latter approach in this work.Nutrient limitation was studied by calculatingdifferent nutrient ratios – totalnitrogen:phosphorus, inorganic nitrogen:phosphorus,and nutrient balance ratio. Results showed thatphosphorus usually limited primary production onlynear the coast line. In the middle zone of theArchipelago Sea the limiting factor variedtemporally. Outer in the open sea nitrogen limitedprimary production during most of the year.Phosphorus limited phytoplankton growth especiallyin spring and in summer and nitrogen in late summerand in autumn. Our results suggested that nitrogenis an important limiting nutrient in the ArchipelagoSea. In recent years when the eutrophication hasproceeded there has been a shift from productionlimitation by both nutrients to limitation bynitrogen alone. But if we want to define andcharacterize the nutrient limitation of the entireecosystem of the Archipelago Sea, budgets have to becalculated for both N and P and internal recyclingmust be taken into account as well as externalsupply of nutrients and loss processes.
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    Biogeochemistry 37 (1997), S. 63-75 
    ISSN: 1573-515X
    Keywords: nutrient limitation ; soil development ; nitrogen ; phosphorus ; chronosequence ; Hawai'i
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Walker & Syers (1976) proposed a conceptual model that describesthe pattern and regulation of soil nutrient pools and availability during long-term soil and ecosystem development. Their model implies that plantproduction generally should be limited by N on young soils and by P on oldsoils; N and P supply should more or less equilibrate onintermediate-aged soils. We tested the application of this model to nutrientlimitation, using a well characterized substrate age sequence in Hawaiianmontane rain forest. Earlier experiments had evaluated nutrient limitationin forests on a young (300 y) and an old (4,100,000 y) substrate on the samedevelopmental sequence; N alone limited tree growth on the youngsubstrate, while P alone did so on the old one. An additional fertilizerexperiment based on replicated treatments with N, P, and all othernutrients combined, applied in individually and in all factorialcombinations, was established in an intermediate-aged site in theLaupahoehoe Forest Reserve, Hawaii. Here, diameter increments of thedominant tree Metrosideros polymorpha increased slightly with Nadditions, and nearly doubled when N and P were added together.Additions of elements other than N and P had no significant effecton growth. These results show that N and P had equilibrated (relativeto plant requirements) in the intermediate aged site. Together withthe earlier experiments, these results suggest that the Walker and Syersmodel provides a useful starting point for explaining the nature anddistribution of nutrient limitation in forest ecosystems.
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  • 80
    ISSN: 1573-515X
    Keywords: aluminium ; forest soils ; lysimeters ; nitrification ; nitrogen ; roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The effects of enhanced (NH4 2SO4deposition on soil solution cation and anion concentrations and annualionic fluxes were followed using a standardised experimental protocolin six European coniferous forests with contrasting soil types, pollutioninputs and climate. Native soil cores containing a ceramic suction cupwere installed in the field, roofed and watered every two weeks withlocal throughfall or local throughfall with added(NH4)2SO4 at 75 kgNH4 +-N ha-1 a-1. Livingroot systems were established in half of the lysimeters.Untreated throughfall NH4 +-N deposition at thesites ranged from 3.7 to 29 kg ha-1 a-1Soil leachates were collected at two weekly intervalsover 12 months and analysed for volume, andconcentrations of major anions and cations. Increasesin soil solution NO3 - concentrations inresponse to N additions were observed after 4–9months at three sites, whilst one sandy soil with highC:N ratio failed to nitrify under any of thetreatments. Changes in NO3 - concentrationsin soil solution controlled soil solution cationconcentrations in the five nitrifying soils, withAl3+ being the dominant cation in the more acidsoils with low base saturation. The acidification responses ofthe soils to the (NH4 2SO4additions were primarily related to the ability of thesoils to nitrify the added NH4 +. pH and soiltexture seemed important in controllingNH4 + leaching in response to the treatments,with two less acidic, clay/clay loam sites showingalmost total retention of added NH4 +, whilstnearly 75% of the added N was leached asNH4 + at the acid sandy soils. The presenceof living roots significantly reduced soil solutionNO3 - and associated cation concentrations attwo of the six sites. The very different responses of the sixsoils to increased (NH4)2SO4deposition emphasise that the establishment of N critical loadsfor forest soils need to allow for differences in N storagecapacity and nitrification potential.
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  • 81
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    Biogeochemistry 39 (1997), S. 1-13 
    ISSN: 1573-515X
    Keywords: 226Ra accumulation ; background level ; natural zones ; terrestrial animals ; nitrogen ; rain forest ; slash-and-burn agriculture ; solutes ; tropical
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Radium (226Ra) accumulation by terrestrial animalswas studied in the territory of the former USSR. Concentrations of226Ra in different soils, plants and animals aresimilar to the background level. For soils it fluctuates in range0.5–1.5 × 10-12 gg-1 (18.3–55 Bq/kg), for plantsbetween 0.1–1.7 × 10-12 gg-1 (3.7–62.1 Bq/kg), and for animals– 0.02–1.5 ×10-12 g g-1(0.73–55 Bq/kg). There were no significant differences in226Ra concentration between animals of different groups. Themaximal 226Ra concentration is marked for the woodlouse Hemilepistus aphganicus from the Badkhyz reserve in Turkmenistan– 1.49 × 10-12g g-1 of dwt (54.4 Bq/kg), the minimal one– for Colorado beetle Leptinotarsadecemlineata from the vicinity of Moscow – 0.02× 10-12 gg-1 of dwt (0.73 Bq/kg). Concentration ratios for linkssoil – plant, plant – animal andprey – carnivore are usually close to 1. A hypothesison the similarity of 226Raconcentration in different animals is discussed.
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  • 82
    ISSN: 1573-2932
    Keywords: nitrogen ; acidification ; Adirondack Mountains ; atmospheric deposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Assessments of the aquatic effects of acidic deposition have focused on sulfur, as have recent efforts to control the emissions of acidifying compounds. Nitrogen dynamics were excluded from most acidic deposition modeling studies because it was believed that terrestrial ecosystems strongly retain N and because modeling N is a more formidable task than modeling S due to the influence of complex biological processes on N cycling. Re-examination of available data for the Adirondack Mountains of New York suggests that N deposition may be contributing to both chronic and episodic acidification of freshwaters to a greater extent than is generally believed. Previous research concluded that N has played a limited role in acidification processes in these lakes, based on regional averages of chronic chemistry. However, it is now known that historic acidification responses have been spatially variable within the Adirondack Mountains and that the declines in lakewater pH have been less than previously believed. Lakewater NO3 − concentrations are commonly in the range of 5 to 25 μeq L−1 on a chronic basis in portions of the Adirondack region that have experienced significant chronic acidification. These NO3 − concentrations correspond in magnitude to inferred historical acidification. Furthermore, the relative importance of NO3 − as an agent of acidification increases dramatically during snowmelt when conditions are most toxic to fish. The consequence of not addressing N in formulating acidification recovery strategies for the Adirondacks includes the likelihood that we will overestimate the response of surface water to the mandated sulfur emissions reductions.
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  • 83
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    Water, air & soil pollution 96 (1997), S. 329-345 
    ISSN: 1573-2932
    Keywords: beech ; biomonitoring ; nitrogen ; solid particles ; spruce ; sulphur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The foliage contents of Stotal and Ntotal were determined in 405 Permanent monitoring plots in four National Parks and sixteen Landscape Protection Areas and were compared with 1483 Permanent monitoring plots on Slovakia. The foliage content of S ranged from 0.72 to 6.77 g kg−1 in hardwoods and from 0.98 to 4.3 g kg−1 in softwoods. A critical increase was determined in one National Park and in four Landscape Protection Areas. The foliage content of N ranged from 9.7 to 48.7 g kg−1 in softwoods, and from 7.8 to 51.4 g kg−1 in hardwoods. An insufficient level of N was determined in one Landscape Protection Area and a critical increase in four Landscape Protection Areas. The sulphur to nitrogen ratio ranged from 0.08 to 0.176 in softwoods, and from 0.078 to 0.253 in hardwoods. The foliage surface on hitherto explored four National Parks and sixteen Landscape Protection Areas in network 4×4 km was covered by all categories of rigid fallout particles. Fly-ash from coal combustion were observed each of National Parks and Landscape Protection Areas by scanning electron microscopy. Mycelia and spore, mineral matters, sand and soil particles were present on all National Parks and Landscape Protection Areas. Particles derived from aluminium production were observed at three Landscape Protection Areas. Particles derived from cement production and from lime works were observed at two National Parks and six Landscape Protection Areas. Particles derived from ferrous metals industry were observed at each of National Parks and Landscape Protection Areas and particles typical for other technologies were present in three of National Parks and twelve Landscape Protection Areas.
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  • 84
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    Water, air & soil pollution 96 (1997), S. 329-345 
    ISSN: 1573-2932
    Keywords: beech ; biomonitoring ; nitrogen ; solid particles ; spruce ; sulphur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The foliage contents of Stotal and Ntotal were determined in 405 Permanent monitoring plots in four National Parks and sixteen Landscape Protection Areas and were compared with 1483 Permanent monitoring plots on Slovakia. The foliage content of S ranged from 0.72 to 6.77 g kg-1 in hardwoods and from 0.98 to 4.3 g kg-1 in softwoods. A critical increase was determined in one National Park and in four Landscape Protection Areas. The foliage content of N ranged from 9.7 to 48.7 g kg-1 in softwoods, and from 7.8 to 51.4 g kg -1 in hardwoods. An insufficient level of N was determined in one Landscape Protection Area and a critical increase in four Landscape Protection Areas. The sulphur to nitrogen ratio ranged from 0.08 to 0.176 in softwoods, and from 0.078 to 0.253 in hardwoods. The foliage surface on hitherto explored four National Parks and sixteen Landscape Protection Areas in network 4 × 4 km was covered by all categories of rigid fall-out particles. Fly-ash from coal combustion were observed each of National Parks and Landscape Protection Areas by scanning electron microscopy. Mycelia and spore, mineral matters, sand and soil particles were present on all National Parks and Landscape Protection Areas. Particles derived from aluminium production were observed at three Landscape Protection Areas. Particles derived from cement production and from lime works were observed at two National Parks and six Landscape Protection Areas. Particles derived from ferrous metals industry were observed at each of National Parks and Landscape Protection Areas and particles typical for other technologies were present in three of National Parks and twelve Landscape Protection Areas.
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  • 85
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    Water, air & soil pollution 98 (1997), S. 389-399 
    ISSN: 1573-2932
    Keywords: fertilizer ; nitrogen ; pollution ; runoff ; stable isotopes ; sugarcane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In many forested wetlands of Louisiana, surface water quality is being deteriorated by nutrient input from adjacent agricultural production areas. This field study assesses the input of fertilizer N, applied to sugarcane fields, to forested wetlands. The potential use of natural abundance variations in 15N14N ratios for identification and tracing surface water N sources (NH 4 + - and NO3 --N) was evaluated. Runoff and surface water samples were collected from sugarcane fields and bordering forested wetlands (6 stations) over a 16 month period and analyzed for NH 4 + -N, NO 3 - -N, and associated NH 4 + -δ15N and NO 3 - -δ15N ratios. Fertilizer N draining into adjacent forested wetland was estimated to be only a small fraction of the amount applied. Concentrations of NH 4 + - and NO 3 - -N in the collected water samples were low and ranged from 0.02 to 1.79 mg L-1. Isotopic analysis revealed NH 4 + -δ15N and NO 3 - -δ15N means were distinctive and may have the potential to be used as tracers of N contamination. The mean NH 4 + -δ15N value was +18.6 ± 7.1‰ and the NO 3 - -δ15N mean was +8.3 ± 3.1‰. Anomalously high NO 3 - -δ15N values (〉30‰) were attributed to denitrification.
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  • 86
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    Water, air & soil pollution 99 (1997), S. 477-486 
    ISSN: 1573-2932
    Keywords: sediment ; nitrogen ; phosphorus ; organic matter ; cluster analysis ; Gulf of Finland ; estuaries
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Dry weight (DW), ignition loss (IL) and concentrations of total nitrogen (TN) and total phosphorus (TP) of the sediment surface layer (0 to 10 cm, 1 cm slices) were analyzed from 20 sites in the eastern Gulf of Finland. The distance of the sampling sites from the mouth of the River Neva explained the nutrient concentrations of the sediments well, while the effect of water depth was negligible. The increase of TN and the decrease of TP along the transect from the river mouth towards the open Gulf were caused by the diminishing share of allochthonous material supplied from the River Neva. The mean TN concentration of the different accumulation areas was about 40 % higher in the sediment surface than in the deeper layer (9 to 10 cm). The corresponding difference for TP varied from 53 to 56 %. The results suggest considerable netflux of nutrients from sediment to water. The net sediment accumulation of nutrients were estimated as 6.0 g m−2 a−1 of N and 1.7 g m−2 a−1 of P corresponding 22 000 t a− of N and 6 100 t a−1 of P for the whole eastern Gulf.
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  • 87
    ISSN: 1573-2932
    Keywords: nitrogen ; acidification ; Adirondack Mountains ; atmospheric deposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Assessments of the aquatic effects of acidic deposition have focused on sulfur, as have recent efforts to control the emissions of acidifying compounds. Nitrogen dynamics were excluded from most acidic deposition modeling studies because it was believed that terrestrial ecosystems strongly retain N and because modeling N is a more formidable task than modeling S due to the influence of complex biological processes on N cycling. Re-examination of available data for the Adirondack Mountains of New York suggests that N deposition may be contributing to both chronic and episodic acidification of freshwaters to a greater extent than is generally believed. Previous research concluded that N has played a limited role in acidification processes in these lakes, based on regional averages of chronic chemistry. However, it is now known that historic acidification responses have been spatially variable within the Adirondack Mountains and that the declines in lakewater pH have been less than previously believed. Lakewater NO3 - concentrations are commonly in the range of 5 to 25 μeq L-1 on a chronic basis in portions of the Adirondack region that have experienced significant chronic acidification. These NO3 - concentrations correspond in magnitude to inferred historical acidification. Furthermore, the relative importance of NO3 - as an agent of acidification increases dramatically during snowmelt when conditions are most toxic to fish. The consequence of not addressing N in formulating acidification recovery strategies for the Adirondacks includes the likelihood that we will overestimate the response of surface water to the mandated sulfur emissions reductions.
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  • 88
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    Water, air & soil pollution 99 (1997), S. 315-323 
    ISSN: 1573-2932
    Keywords: lake ; sediment ; carbon ; nitrogen ; stable isotope ; fractionation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Stable isotope composition of carbon and nitrogen in the sediment and pore water of a eutrophic freshwater lake was studied. Based on changes in the δ13C and δ15N values of dissolved components and sediment fraction, possible processes involved in the decomposition of sedimentary organic matter are outlined. The relative importance of acetate fermentation and CO2 reduction was estimated using known mathematical models, and ammonia assimilation by methanogenic bacteria is hypothesised to be the main process governing the isotope fractionation of dissolved nitrogen in pore water.
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  • 89
    ISSN: 1573-2932
    Keywords: Adriatic Sea ; nutrients ; benthic fluxes ; carbon ; nitrogen ; silicon ; phosphorus ; budgets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Benthic fluxes of dissolved inorganic N, Si and P nutrients, alkalinity, dissolved inorganic C (DIC), and O2 from sediments in the Gulf of Trieste (northern Adriatic, Italy) were measured monthly in the period September 1995 - August 1996 using in situ incubated light benthic chambers. The highest efluxes of DIC, NH4+, PO43-, Si(OH)4, and NO3- influxes encountered in late summer - early autumn were the consequence of degradation of benthic microalgae, and in autumn mostly of sedimented phytoplankton. High NO3- efflux was observed in spring. Only NH4+ and Si(OH)4 fluxes were significantly correlated with temperature. This correlation suggests that the rate of downward input and the quality and quantity of sedimentary organic matter (autochthonous and allochthonous) were superimposed on the temperature fluctuations. High DIC, NH4+ and Si(OH)4 effluxes observed in July 1996 were due to the late spring - early summer degradation of sedimentary organic matter produced by benthic microalgae, while the autumn phytoplankton bloom was quickly reflected in enhanced benthic fluxes due to higher temperature. Significant correlations between NH4+, PO43- and Si(OH)4 fluxes suggested their parallel regeneration and utilization at the sediment-water interface. The nutrient fluxes were linked to O2 consumption, suggesting that aerobic oxidation processes were important at the sediment-water interface in the Gulf. The N, P and Si nutrients released from sediment pore waters are probably utilized in benthic microalgal and bottom-water primary production. This indicates that pelagic and benthic communities in the central part of the Gulf of Trieste function relatively independently of each other.
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  • 90
    ISSN: 1573-2932
    Keywords: Cystoseira myrica ; Red Sea ; coral reefs ; nitrogen ; phosphorus ; pollution ; eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Elevated phosphate concentrations at Safaga and Quseir, Red Sea, Egyptian coast, wererelated to some ecological and nutritional aspects of the macro-alga Cystoseira myrica during1985. The results obtained were compared with those recorded for a non-polluted control site atGhardaqa. Chemical analysis of surface seawater samples revealed that the concentrations of dissolvedphosphate at Safaga and Quseir were 3 and 20 times as much as that at Ghardaqa, respectively.The standing crop of C. myrica showed two peaks in spring and autumn. Maximum biomass yield(491 g m-2) and number of individuals (127 m-2) of C. myrica were recorded in the spring at theGhardaqa site. Quseir C. myrica samples contained significantly higher protein-N, total-N andtotal-P values but lower carbohydrate values than the Safaga and Ghardaqa samples (LSD at 1%level). Tissue N:P molar ratios for C. myrica, Laurencia papillosa and Ulva lactuca collected fromGhardaqa ranged from 68-98, compared to 30-59 for the same species collected at thephosphorus polluted sites at Safaga and Quseir. The carbon levels and the C:N ratios of C. myricaexhibited no significant variations in the three studied sites. However, the C:P ratios of Ghardaqaplants were significantly higher than those of Safaga and Quseir.
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  • 91
    ISSN: 1573-2932
    Keywords: Cystoseira myrica ; Red Sea ; coral reefs ; nitrogen ; phosphorus ; pollution ; eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Elevated phosphate concentrations at Safaga and Quseir, Red Sea, Egyptian coast, were related to some ecological and nutritional aspects of the macro-algaCystoseira myrica during 1985. The results obtained were compared with those recorded for a non-polluted control site at Ghardaqa. Chemical analysis of surface seawater samples revealed that the concentrations of dissolved phosphate at Safaga and Quseir were 3 and 20 times as much as that at Ghardaqa, respectively. The standing crop ofC. myrica showed two peaks in spring and autumn. Maximum biomass yield (491 g m−2) and number of individuals (127 m−2) ofC. myrica were recorded in the spring at the Ghardaqa site. QuseirC. myrica samples contained significantly higher protein-N, total-N and total-P values but lower carbohydrate values than the Safaga and Ghardaqa samples (LSD at 1% level). Tissue N:P molar ratios forC. myrica, Laurencia papillosa andUlva lactuca collected from Ghardaqa ranged from 68–98, compared to 30–59 for the same species collected at the phosphorus polluted sites at Safaga and Quseir. The carbon levels and the C:N ratios ofC. myrica exhibited no significant variations in the three studied sites. However, the C:P ratios of Ghardaqa plants were significantly higher than those of Safaga and Quseir.
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  • 92
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    Water, air & soil pollution 99 (1997), S. 315-323 
    ISSN: 1573-2932
    Keywords: lake ; sediment ; carbon ; nitrogen ; stable isotope ; fractionation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Stable isotope composition of carbon and nitrogen in the sediment and pore water of a eutrophic freshwater lake was studied. Based on changes in the δ11C and δ15N values of dissolved components and sediment fraction. possible processes involved in the decomposition of sedimentary organic matter are outlined. The relative importance of acetate fermentation and CO2 reduction was estimated using known mathematical models, and ammonia assimilation by methanogenic bacteria is hypothesised to be the main process governing the isotope fractionation of dissolved nitrogen in pore water.
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  • 93
    ISSN: 1572-9788
    Keywords: DNA markers ; genetic linkage map ; marker-assisted breeding ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We have developed an RFLP framework map with 146 RFLP markers based on a doubled haploid population derived from a cross between an indica variety IR64 and a japonica variety Azucena. The population carries 50.2% of IR64 loci and 49.8% of Azucena loci, indicating an equal amount of genetic materials from each parent has been transmitted to the progenies through anther culture. However, some markers show segregation distortion. These distorted marker loci are located on 10 chromosomal segments. Using this map we were able to place 8 isozymes, 14 RAPDs, 12 cloned genes, 1 gene for brown planthopper (BPH) resistance, and 12 QTLs for grain length, grain width and length/width ratio onto rice chromosomes. The major gene for BPH resistance was mapped on chromosome 12 near RG463 and isozyme Sdh-1. Most of the QTLs identified for the three grain characters were closely linked on chromosomes 1, 2, 3 and 10. We concluded that the RFLP framework map presented here will be useful for mapping other genes segregating in this doubled haploid population. Thus rapid generation of doubled haploid lines and their unbiased segregation make it very attractive for gene mapping.
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  • 94
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    Photosynthetica 34 (1997), S. 401-408 
    ISSN: 1573-9058
    Keywords: biomass ; carotenoids ; catalase ; chlorophyll ; flavonoids ; Oryza sativa ; peroxidase ; phenols ; stomatal conductance ; transpiration rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a field experiment with rice (Oryza sativa L. cv. Saket 4) grown under ambient and supplemental ultraviolet-B (UV-B) radiation at 20 % ozone depletion, differences in gas exchange, concentrations of photosynthetic pigments, anthocyanins and flavonoids, biomass accumulation, catalase and peroxidase activities, and contents of ascorbic acid and phenol were determined. Decline in photosynthesis was associated with reductions in stomatal conductance and concentrations of photosynthetic pigments. Enhanced UV-B radiation (eUV-B) increased the contents of flavonoid and phenolic compounds in leaves. Peroxidase activity increased and catalase activity was always lower at eUV-B. The total plant biomass decreased at eUV-B.
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  • 95
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    World journal of microbiology and biotechnology 13 (1997), S. 595-596 
    ISSN: 1573-0972
    Keywords: Algal biomass ; Aulosira yield ; Brassica campestris ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This study points to the utility of Aulosira fertilissima and algal biomass of the Upper and Lower lake of Bhopal as a cheap and effective green manure for rice and mustard.
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  • 96
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    Biologia plantarum 39 (1997), S. 447-451 
    ISSN: 1573-8264
    Keywords: D-arginine ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Suspension-cultured rice cells growth was markedly inhibited and ammonium content increased when rice cells were deprived of phosphate. When rice cells were cultured at increasing concentrations of ammonium chloride, ammonium content increased, however, no significant inhibition of cell growth was observed. Addition of D-arginine, an inhibitor of putrescine biosynthesis, resulted in a complete recovery of growth in rice cells under phosphate deprivation, but did not decrease the content of ammonium. Our results indicate that the growth inhibition induced by phosphate deprivation is not associated with ammonium accumulation.
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  • 97
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    Biologia plantarum 40 (1997), S. 597-603 
    ISSN: 1573-8264
    Keywords: chlorophyll ; Oryza sativa ; starch ; soluble sugars
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pre-submergence reserve saccharides was found adequate to substantiate the survival of tolerant cultivar under flooding. Survival percentage declined in both tolerant and susceptible cultivars with less saccharide content. However, it was more apparent in susceptible cultivar. Plant height, fresh and dry mass of shoots, leaf mass/area ratio and starch content per plant before flooding showed significant positive association with submergence tolerance. Hence, the improved seedling vigour could be used to increase submergence tolerance.
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  • 98
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    Biologia plantarum 40 (1997), S. 295-298 
    ISSN: 1573-8264
    Keywords: cultivar differences ; growth parameters ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For screening tolerance of six cultivars of rice (Oryza sativa) to different concentrations of nickel (0, 6, 12, 18, 24 and 30 μg dm3), growth parameters (root and shoot length, root and shoot dry matter production) and root and shoot tolerance indexes were tested after 7, 10 and 13 d of treatment. In presence of nickel in nutrient solution, root growth of cv. Nilgiri was enhanced, while in cvs. Subhadra, Khandagiri, Rudra, Sankar and Annapurna it decreased. The root tolerance index (RTI) and shoot tolerance index (STI) in cv. Nilgiri were high; cvs. Sankar and Khandagiri, however, showed a low RTI. Based on the standard growth parameters, six cultivars of rice were ranked in respect of their tolerance to nickel: Nilgiri 〉 Annapurna 〉 Subhadra 〉 Khandagiri 〉 Rudra 〉 Sankar.
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  • 99
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    Biologia plantarum 40 (1997), S. 305-309 
    ISSN: 1573-8264
    Keywords: micro-callus ; Oryza sativa ; particle-bombardment ; suspension culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Culture conditions for a fine dispersion of plated cells of Oryza sativa L. cv. IR 20, have been worked out. These plated cells developed microcalli containing large number of somatic embryos and subsequently plantlets. By using single cells and clusters of 2 - 4 cells, an efficient DNA-delivery by microprojectile bombardment into cells and its transient expression were assessed by employing a plasmid construct containing β-glucuronidase gene.
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  • 100
    ISSN: 1573-8264
    Keywords: Oryza sativa ; osmoprotectant ; PEG ; seedlings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When seedlings of two rice (Oryza sativa L.) cultivars Ratna and Jaya were raised under 100 and 500 µM cadmium nitrate in the medium, a high proline content was noted in Cd2+ stressed seedlings compared to controls. Seedlings grown under 500 µM Cd(NO3)2 maintained increased proline level compared to non-stressed seedlings. Kinetic properties of RNase extracted from control grown and Cd2+ stressed seedlings showed a marked alteration in Km due to Cd2+ treatment. The RNase isoforms were purified from 15-d-old rice seedlings with a total purification of 22.25 fold and 74.75 % yield using conventional biochemical techniques. Three RNase isoforms, namely I, II and III were eluted from DEAE-Sephacel column. The isoform RNase II had Km value of 3.2 mg(RNA) cm-3. The in vitro osmotic stress created by incorporation of PEG in the enzyme assay medium led to decreased affinity of enzyme towards its substrate with increase in Km. This loss in affinity was partially restored by the addition of 1 M proline in the assay medium, suggesting the possible protective role of proline on RNase under osmotic stress.
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