ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    ACCLIMATIVE RESPONSE TO TEMPERATURE STRESS IN HIGHER PLANTS: Approaches of Gene Engineering for Temperature Tolerance (2002)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology and Plant Molecular Biology 53 (2002), S. 225-245 
    Details
    ISSN: 1040-2519
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Notes: Abstract Temperature stresses experienced by plants can be classified into three types: those occurring at (a) temperatures below freezing, (b) low temperatures above freezing, and (c) high temperatures. This review outlines how biological substances that are deeply related to these stresses, such as heat-shock proteins, glycinebetaine as a compatible solute, membrane lipids, etc., and also detoxifiers of active oxygen species, contribute to temperature stress tolerance in plants. Also presented here are the uses of genetic engineering techniques to improve the adaptability of plants to temperature stress by altering the levels and composition of these substances in the living organism. Finally, the future prospects for molecular breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.arplant.53.100201.160729
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Deletion of the gene encoding cytochrome b562 from Rhodobacter sphaeroides (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 120 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Cytochrome b562 is a membrane bound di-heme cytochrome previously isolated and characterized from the membranes of photosynthetically grown Rhodobacter sphaeroides. Although originally suggested to be a component of the bc1 complex, subsequent work, including the sequence of the gene encoding the protein, indicates that this is not the case. More recently, it has been proposed to be a component of complex II (succinate-ubiquinol oxidoreductase). In an effort to determine the function of this cytochrome, the gene encoding the protein has been deleted from the chromosome, and the resulting strain has been examined. The results show that this cytochrome is not as essential component of complex II, the bc1 complex, or any of the respiratory oxidases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07015.x
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Expression of a gene for plastid ω-3 fatty acid desaturase and changes in lipid and fatty acid compositions in light- and dark- grown wheat leaves (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 96 (1996), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The leaves of monocotyledonous plants create a developmental sequence of cells and plastids from the base to the apical portion. We investigated fatty-acid and lipid compositions in successive leaf sections of light- and dark-grown wheat (Triticum aestivum L. cv. Chihoku) seedlings. The most notable change in the fatty acid composition was the increase of linolenic acid (18:3) with maturation of leaf cells, which occurred both in light- and dark-grown leaf tissues. In light-grown leaves, the increase of 18:3 with maturation was mainly attributed to the increase of monogalactosyldiacylglycerol (MGD) and also to the increase of the 18:3 level of MGD. In dark-grown leaves, the increase of 18:3 in the leaf apex was caused by the increase of the levels of MGD and digalactosyldiacylglycerol (DGD) and also by the increase of the 18:3 levels of within these two lipids. Since MGD and DGD are mainly found in plastid membranes, these findings indicate that both the synthesis of galactolipids and the formation of 18:3 these lipids take place during plastid development. The plastid ω-3 fatty acid desaturase is responsible for the formation of 18:3 in plastid membrane lipids. To investigate the regulation of desaturation, we isolated a gene for wheat plastid ω-3 fatty acid desaturase (TaFAD7). The mRNA level of TaFAD7 in light-grown leaves was much higher than that in dark-grown leaves. During the greening of etiolated leaves the level of TaFAD7 mRNA increased significantly, accompanied by an increase of the 18:3 level of total fatty acids. On the other hand, the levels of TaFAD7 mRNA were almost the same in all the leaf sections of both light- and dark-grown leaf tissues. These results suggest that the effect of the expression of the TaFAD7 gene on the increase of the 18:3 level is different between the leaf development under continuous light- or dark-conditions and the light-induced greening process of etiolated leaves. The increase of 18:3 content of MGD (or MGD and DGD) with maturation is apparently regulated not solely by the level of TaFAD7 mRNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1996.tb00214.x
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Temperature-sensitive Arabidopsis mutant defective in 1-deoxy-d-xylulose 5-phosphate synthase within the plastid non-mevalonate pathway of isoprenoid biosynthesis (2000)
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 108 (2000), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The temperature-sensitive mutant of Arabidopsis, chs5, developed chlorotic leaves at restrictive temperatures (15°C), but almost normal green leaves at permissive temperatures (22°C). At the restrictive temperature, the chs5 mutation blocked the accumulation of chlorophylls and carotenoids. A temperature-shift analysis revealed that the manifestation of the chlorotic phenotype occurred in young leaf tissues, but did not in mature leaf tissues. Genetic and sequence analysis demonstrated that the chs5 mutation was caused by a single-base change in the coding region of a recently identified CLA1 gene. The CLA1 gene exhibited a high sequence similarity to the genes encoding 1-deoxy-d-xylulose 5-phosphate synthase (DXS) localized to the non-mevalonate pathway, which was recently discovered in bacteria and higher plants. In addition, the application of 1-deoxy-d-xylulose, the free sugar of 1-deoxy-d-xylulose 5-phosphate, rescues the defect in the chs5 mutant. These results indicated that the chlorotic phenotype of the chs5 mutant was caused by a defect in DXS activity and that DXS functions preferentially at an early stage of leaf cell development. A transiently expressed green fluorescent protein fused with the CLA1 transit peptide was localized within the chloroplasts in the green cultured cells of tobacco, which suggests that the putative localization of the non-mevalonate pathway is in plastids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2000.108001019.x
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Characterization of a rice mutant having an increased susceptibility to light stress at high temperature (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 89 (1993), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of light irradiation at high temperature was investigated in a rice mutant, spl-2, which is sensitive to solar radiation. Dead spots appeared on the mutant leaves when cultured at a high temperature (40°C) under strong white-light illumination (15 W m−2). A similar damage was also observed in the wild-type leaves under the same conditions when the plants were preincubated in the dark for one day. Preillumination with weak light (6 W m−2) lessened the irradiation-induced damage in the wild-type. These observations suggest that in rice plants the acclimatization to weak light has a protective effect against strong irradiation at high temperature, and the spl-2 locus participates in the mechanisms of the acclimatization. The action spectrum for the irradiation-induced damage at the high temperature (40°C) in the spl-2 leaves indicated that the maximum damage occurred at around 480 nm and, in a lesser extent, at around 680 nm. Activity of the O−2− and H2O2−scavenging enzymes in the spl−2 leaves were almost the same or somewhat higher than those in the wild-type after irradiation with strong white light (15 W m−2) at 40°C, whereas the content of ascorbic acid in the spl−2 decreased significantly compared with the wild type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1993.tb05287.x
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Role of ω-3 fatty acid desaturases in the regulation of the level of trienoic fatty acids during leaf cell maturation (1996)
    Springer
    Planta 199 (1996), S. 439-442 
    Details
    ISSN: 1432-2048
    Keywords: Arabidopsis ; ω-3 fatty acid desaturase ; Leaf cell maturity ; Linolenic acid ; Lipid transfer ; Mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Trienoic fatty acids, namely α-linolenic acid and hexadecatrienoic acid, present in leaf lipids are produced by ω-3 fatty acid desaturases located in the endoplasmic reticulum and plastid membranes. The changes in the level of trienoic fatty acids during leaf maturation were investigated in wild-type plants of Arabidopsis thaliana (L.) Heynh. and in the fad7 mutant deficient in the activity of a plastid ω-3 desaturase. The levels of trienoic fatty acids increased in 26 °C- and 15 °C-grown wild-type plants with maturation of leaves. The increase in trienoic fatty acids was mainly due to galactolipids enriched in plastid membranes. In addition, the relative levels of trienoic fatty acids in major glycerolipids, including phospholipids enriched in the endoplasmic reticulum membranes, also increased with leaf maturation. By contrast, when the fad7 mutant was grown at 26 °C, the relative levels of trienoic fatty acids in individual lipids decreased with leaf maturation. The decreases in the levels of trienoic fatty acids, however, were alleviated when the fad7 mutant was grown at 15 °C. These results suggest that the plastid ω-3 desaturase plays a major role in increasing the levels of trienoic fatty acids with leaf maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195737
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Tissue-specific and light-responsive regulation of the promoter region of the Arabidopsis thaliana chloroplast ω-3 fatty acid desaturase gene (FAD7) (1995)
    Springer
    Plant molecular biology 29 (1995), S. 599-609 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; chloroplast ; gene expression ; ω-3 fatty acid desaturase ; promoter ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Arabidopsis FAD7 gene encodes a chloroplast ω-3 fatty acid desaturase that catalyzes the desaturation of lipid-linked dienoic fatty acids (18:2 and 16:2). An 825 bp FAD7 promoter fragment upstream from the transcriptional start point contained several short sequences which were homologous to the cis-elements (box II, G-box, etc.) conserved in many light-responsive genes. We introduced the FAD7 promoter fused to the β-glucuronidase (GUS) or the luciferase (LUC) reporter gene into tobacco plants. The −825 promoter sequence conferred tissue-specific and light-responsive expression to both these reporter genes in transgenic tobacco, indicating that these expressions of the FAD7 gene were regulated mainly at the transcriptional level. Histochemical GUS staining showed that the activity of the FAD7 promoter is restricted to the tissues with chloroplast-containing cells although the staining was noticeably absent in the chloroplast-containing cells associated with vascular systems. The 5′ deletion experiments of the promoter revealed that the −362/ −166 region, containing two putative box II sequences, was responsible for the tissue-specific and light-responsive expression of the FAD7 gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020987
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Structure, chromosomal location and expression of a rice gene encoding the microsome ω-3 fatty acid desaturase (1997)
    Springer
    Plant molecular biology 33 (1997), S. 493-502 
    Details
    ISSN: 1573-5028
    Keywords: chromosome location ; linolenic acid ; microsome ; ω-3 fatty acid desaturase ; Oryza sativa ; transgenic hairy roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ω-3 fatty acid desaturases are membrane-bound enzymes catalyzing the conversion of linoleic acid to linolenic acid in lipids, and are located both in the microsome and plastid envelopes as two different isoforms. A cDNA encoding the microsome ω-3 fatty acid desaturase (OsFAD3) and the corresponding genomic clone were isolated from rice (Oryza sativa L.). The OsFAD3 gene was composed of 8 exons and 7 introns. A microsatellite was present in the second exon of the OsFAD3 gene, showing polymorphism between Indica and Japonica rice varieties. The mapping of this microsatellite showed that the OsFAD3 gene was located on chromosome 11. Expression of the OsFAD3 cDNA in tobacco hairy root tissues and subsequent analysis of fatty acid compositions demonstrated the activity of the microsome ω-3 fatty acid desaturase. The OsFAD3 mRNA was abundant in root tissues, but was hardly detectable in leaves. In root tissues, a high level of the OsFAD3 mRNA was observed at 15 °C and 20 °C, with its level decreasing markedly at temperatures below 10 °C. The accumulation of the OsFAD3 mRNA in leaf tissues remained at quite low levels, both at normal growth temperatures and at chilling temperatures. Similar temperature responses of the OsFAD3 gene were observed both in chilling- tolerant and in chilling-intolerant rice cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005726210977
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Two maize genes encoding ω-3 fatty acid desaturase and their differential expression to temperature (1998)
    Springer
    Plant molecular biology 36 (1998), S. 297-306 
    Details
    ISSN: 1573-5028
    Keywords: gene cloning ; fatty acid desaturase ; temperature ; Zea mays ; chloroplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated two maize cDNAs and the corresponding genes encoding fatty acid desaturase with Arabidopsis thaliana FAD7 gene as a probe. They shared almost 90% identity at DNA sequence level. Northern analysis revealed that both genes are expressed in leaves, but not in roots at normal temperature- and low temperature-growth condition. The overall level of these transcripts are elevated upon exposure to low temperature. The tissue-specific expression and DNA sequence data indicate that both genes encode plastidic ω-3 fatty acid desaturases. One of them is expressed exclusively at normal temperature but not at 5 °C , whereas the other is expressed inversely. We, therefore, termed them ZmFAD7 and ZmFAD8, respectively. Among other stresses, high-salt treatment induced the accumulation of the ZmFAD7 and ZmFAD8 transcripts in roots but drought had no effect on their expression. Cycloheximide induced the accumulation of the ZmFAD7 transcript in roots. The genomic clones of ZmFAD7 and ZmFAD8 consist of 8 exons and 7 introns as same as in the cases of A. thaliana FAD7 and FAD8 genes and the sizes of the 6 internal exons were identical among them. A phylogenetic analysis of ZmFAD7, ZmFAD8 amino acid sequences and those originated from other plant species is also presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005993408270
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Modification of fatty acid composition by over- and antisense-expression of a microsomal ω-3 fatty acid desaturase gene in transgenic tobacco (1996)
    Springer
    Transgenic research 5 (1996), S. 115-121 
    Details
    ISSN: 1573-9368
    Keywords: ω-3 fatty acid desaturase ; overexpression ; antisense RNA ; linolenic acid ; transgenic tobacco ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ω-3 fatty acid desaturases, which catalyse the conversion of linoleic acid (18:2) to linolenic acid (18:3) in lipids, are located in the microsomes and plastid membranes. Transgenic tobacco plants were produced that express the transcripts of a tobacco microsomal ω-3 fatty acid desaturase gene (NtFad3) in antisense and sense orientations under the control of the cauliflower mosaic virus 35S promoter. The antisense construct has the 0.5-kb fragment of theNtFad3 cDNA containing a 3′-flanking region and a part of the coding region in antisense orientation. The antisense-transformant lines showed decreases of the steady-stateNtFad3 mRNA level to 30% of the control plants. In these lines, the 18:3 content decreased to about 80% in root tissues and to about 70–80% in leaf tissues when compared with the control plants. The sense construct has the 1.4-kb full-length cDNA ofNtFad3. In one of the sense-transformant lines, theNtFad3 mRNA level increased 8 times when compared with that of the control plants. In this line, the 18:3 content increased by about 1.5-fold in root tissues and by about 1.1-fold in leaf tissues. These results indicate that the up- and down-regulation of the transcript level in the microsomal ω-3 fatty acid desaturase gene is useful to modify the 18:3 content in the vegetative tissues of higher plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01969429
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...