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Plant regeneration from protoplasts isolated from primary calli using mature embryos of two Brazilian rice cultivars

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Abstract

A plant regeneration system from rice protoplasts using calli derived from mature embryos was established for the two Brazilian modern rice cultivars IAC-201 and IAC-165. After 30 to 40 days of in vitro culture it was possible to obtain on average 6 million protoplasts per gram of callus. Microscopic selection of embryogenic calli was a key step for protoplast isolation. The production of embryogenic calli increased when L-proline and casein hydrolysate were used in the callus induction medium. The Oc or IR52 nurse cell lines were essential for protoplast division. Different regeneration media were studied and 139 plants were regenerated which set seed. Some of the regenerated plants showed morphological variation such as the presence of awns in spite of the short time of the in vitro culture.

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References

  • Abdullah, R., E.C. Cocking & J.A. Thomson, 1986. Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4: 1087–1093.

    Article  Google Scholar 

  • Aldemita, R. & F.J. Zapata, 1991. Proline and high NH4 + concentration on the anther culture of rice. Kimika 7: 31–36.

    Google Scholar 

  • Chu, C., 1978. The N6 medium and its applications to anther culture of cereal crops. In: Proceedings of Symposium on Plant Tissue Culture, pp. 43–50. May 25–30. Peking, China.

  • Ella, E.S. & F.J. Zapata, 1993. Effect of maltose and gelling agent on protoplast culture response in indica rice. Intern Rice Res Newslett 17: 5–6.

    Google Scholar 

  • Frearson, E.M., J.B. Power & E.C. Cocking, 1973. The isolation, culture and regeneration of petunia leaf protoplasts. Dev Biol 33: 130–137.

    Article  PubMed  CAS  Google Scholar 

  • Fujimura, T., M. Sakurai, H. Akagi, T. Negishi & A. Hirose, 1985. Regeneration of rice plants from protoplasts. Plant Tissue Culture Lett 2: 74–75.

    Google Scholar 

  • Gamborg, O.L., R.A. Miller & K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158.

    Article  PubMed  CAS  Google Scholar 

  • Ghosh Biswas, G.C. & F.J. Zapata, 1993. High-frequency plant regeneration from protoplasts of indica rice (Oryza sativa L.) using maltose. J Plant Physiol 141: 470–475.

    Google Scholar 

  • Hoagland, D.R. & D.I. Arnon, 1950. The water culture methods for growning plants without soil. Agric Exp Bta (Circ 347).

  • Koetje, D.S., H.D. Grimes, Y-C. Wang & T.K. Hodges, 1989. Regeneration of rice (Oryza sativa L.) from primary callus derived from immature embryos. J Plant Physiol 135: 184–190.

    CAS  Google Scholar 

  • Kyozuka, J., Y. Hayashi & K. Shimamoto, 1987. High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206: 408–413.

    Article  CAS  Google Scholar 

  • Kyozuka, J., E. Oto & K. Shimamoto, 1988. Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Theor Appl Genet 76: 887–896.

    Article  Google Scholar 

  • Lee, L., R.E. Schroll, H.D. Grimes & T.K. Hodges, 1989. Plant regeneration from indica rice (Oryza sativa L.) protoplasts. Planta 178: 325–333.

    Article  Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth requirements of tobacco tissue cultures. Physiol Plant 15: 473–497.

    Article  CAS  Google Scholar 

  • Ohira, K., K. Ojima & A. Fujiwara, 1973. Studies on the nutrition of rice cell culture I. A simple defined medium of rapid growth in suspension culture. Plant Cell Physiol 14: 1113–1121.

    CAS  Google Scholar 

  • Rueb, S., M. Leneman, R.A. Schilperoort & L.A.M. Hensgens, 1994. Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.). Plant Cell, Tissue & Organ Cult 36: 259–264.

    Article  Google Scholar 

  • Toriyama, K., K. Hinata & T. Sasaki, 1986. Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor Appl Genet 73: 16–19.

    Article  Google Scholar 

  • Torrizo, L.B. & F.J. Zapata, 1992. High efficiency plant regeneration from protoplasts of the indica cultivar IR58. J Exp Bot 43: 1633–1637.

    Google Scholar 

  • Wu, C. & F.J. Zapata, 1992. Plant regeneration from protoplasts isolated from primary callus of four japonica rice (Oryza sativa L.) varieties. Plant Sci 86: 83–87.

    Article  Google Scholar 

  • Zapata, F.J. & E.S. Ella, 1988. Specific gravity of the grain — A factor to consider in rice tissue culture. J Plant Physiol 132: 294–297.

    Google Scholar 

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Author notes

  1. Francisco J. Zapata-Arias

    Present address: Plant Breeding Unit, FAO/IAEA Agriculture and Biotechnology Laboratory, A-2444, Seibersdorf, Austria

Authors and Affiliations

  1. Departmento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil

    Daniel S. Moura, Francisco J. Zapata-Arias & Akihiko Ando

  2. Seçao de Radiogenética, Centro de Energia Nuclear na Agricultura (CENA), Universidade de Sao Paulo, Piracicaba, Sao Paulo, Brasil

    Augusto Tulmann Neto

Authors
  1. Daniel S. Moura
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  2. Francisco J. Zapata-Arias
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  3. Akihiko Ando
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  4. Augusto Tulmann Neto
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Moura, D.S., Zapata-Arias, F.J., Ando, A. et al. Plant regeneration from protoplasts isolated from primary calli using mature embryos of two Brazilian rice cultivars. Euphytica 94, 01–05 (1997). https://doi.org/10.1023/A:1002962724080

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  • DOI: https://doi.org/10.1023/A:1002962724080

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