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  • 1
    Electronic Resource
    Electronic Resource
    Transgene risk is not too low to be tested (1998)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 394 (1998), S. 715-715 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SirIn the Newsstory “Organic farmer takes gene battle to court”, the view is reported that “the probability of cross-pollination, and its effects on the environment and food safety, is too small to be studied effectively”. But the probability of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/29384
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of Taxa in the Genus shape Beta using ITS1 Sequence Information (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 147-155 
    Details
    ISSN: 1572-9818
    Keywords: allele-specific PCR ; Beta ; ITS1 ; plant identification ; rDNA ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sequence variation in the ITS1 locus of the nuclear ribosomal DNA in beets has previously been used to reconstruct phylogeny of the species in the genus Beta. We have developed protocols that allow the identification of Beta taxa by use of taxon-specific primers. Beta sections, species and subspecies can be identified. Differences within the ITS1 region of a single base can be exploited for species identification. The results from this study not only provide effective methods for wild beet identification, but also indicate the potential use of the techniques in other crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007416817736
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  • 3
    Electronic Resource
    Electronic Resource
    Rust infections of some Allium species: An assessment of germplasm for utilizable rust resistance (1990)
    Springer
    Euphytica 49 (1990), S. 99-109 
    Details
    ISSN: 1573-5060
    Keywords: Allium ; Puccinia allii ; Puccinia mixta ; Uromyces ambiguus ; leek rust ; resistance ; germplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Isolates of three species of rust (Puccinia allii, P. mixta and Uromyces ambiguus) were each inoculated onto a range of potential host plants, selected to include the major European crop Allium species together with the commoner wild species in the section Allium. Measurements of incubation period, lesion type, latent period and pustule quantity were made in a single environment. Each isolate was able to sporulate on a range of Allium species. There was evidence of specialisation to particular sections of the genus; all the leek isolates performed well on the majority of hosts in section Allium, whilst the chive isolate grew poorly on most members of that section. Exceptions to this are exemplified by A. fistulosum being attacked by all three rust species and A. sativum being attacked by both leek and chive isolates. A continuum of reaction types was found which, for the leek isolates, ranged from the low levels of quantitative resistance in leek cultivars, through higher quantitative levels within the A. ampeloprasum complex, to qualitative resistance in several interactions in non-host sections of Allium, where the pathogen was able to infect and form colonies of some size without sporulating.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027259
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  • 4
    Electronic Resource
    Electronic Resource
    Regeneration of plants from Antirrhinum majus L. callus (1989)
    Springer
    Plant cell, tissue and organ culture 17 (1989), S. 59-70 
    Details
    ISSN: 1573-5044
    Keywords: Antirrhinum majus L. ; plant regeneration ; callus ; tissue culture ; embryoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somaclone production in Antirrhinum majus plants by regeneration of plants from callus cultures has been achieved using three types of explant tissue. Regeneration from mature stem internode-derived callus was extremely poor. Callus derived from seedling shoot tips could be induced to form new shoots in six of seven cultivars tested. Regeneration was achieved in all seven cultivars when callus was produced from segments of hypocotyl and was most effective using agar-solidified medium containing 0.25 mgl-1 naphthoxyacetic acid + 10% coconut milk. In this case, five of the cultivars produced shoots directly, one produced leaves from the petioles of which new shoots emerged, and one regenerated plants chiefly through the production of embryoids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042282
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro adventitious root induction in Antirrhinum majus L. (1991)
    Springer
    Plant cell, tissue and organ culture 27 (1991), S. 77-79 
    Details
    ISSN: 1573-5044
    Keywords: adventitious ; Antirrhinum majus L. ; plant regeneration ; rooting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A broadly applicable method for the successful induction of root systems in a number of cultivars of A. majus has been determined. This involves a double filter-paper bridge with a liquid medium for root induction and allows the transfer of culture-grown plantlets to a glasshouse environment with minimal disturbance to the plant as a whole. 100% survival of transferred plantlets has been achieved with the inclusion of a few simple precautions upon shoot transfer and during the initial stages of plant establishment in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048210
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  • 6
    Electronic Resource
    Electronic Resource
    Population genetic structure in Lens taxa revealed by isozyme and RAPD analysis (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 549-559 
    Details
    ISSN: 1573-5109
    Keywords: AMOVA ; Lens ; population genetic structure ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An understanding of the genetic structure of populations is vital for the formation of optimum collection, conservation and utilization strategies for plant genetic resources. This is of particular importance in the case of in-situ conservation, a strategy gaining in popularity. The population genetic structures of five wild lentil taxa, Lens culinaris subsp. orientalis, L. odemensis, L. ervoides, L. nigricans and L. lamottei were investigated using isozyme electrophoresis and random amplified polymorphic DNA (RAPD). Approximately 20 plants from each of 5 populations per taxon were screened for variation at 11 isozyme loci and using three RAPD primers. Levels of variation were generally low, although considerable variation existed in the levels of diversity found within populations of L. culinaris subsp. orientalis and L. lamottei. Comparison of the results obtained in this study with the results obtained in a previous study indicate that this is a trend occurring across all species. It implies that levels of diversity within populations must be measured and considered prior to targeting of specific populations for in-situ conservation. Analysis of molecular variance of both isozyme and RAPD data revealed that between 78% and 99% of the variation was attributable to between-population differences. Isozyme results from L. lamottei populations were, however, contradictory. Possible explanations for this difference are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008640201896
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  • 7
    Electronic Resource
    Electronic Resource
    Complementary conservation strategies for the genus Coffea: A case study of Mascarene Coffea species (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 565-579 
    Details
    ISSN: 1573-5109
    Keywords: complementary conservation strategies ; Coffea ; in situ ; ex situ ; Mauritius ; Mascarene islands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract No single conservation technique can adequately conserve the full range of genetic diversity of a target species or genepool. To optimize the efficiency of germplasm conservation, a range of conservation techniques needs to be applied simultaneously. In this paper we review the progress made in the application of both in situ and ex situ complementary conservation strategies and techniques to the conservation of the genus Coffea and identify areas where more research work is required. Traditionally, Coffea species have been conserved ex situ as living plants in field genebanks due to their intermediate seed storage behaviour. However, progress made in the field of biotechnology in recent years have made it possible to envisage the use of in vitro, slow growth and cryopreservation for medium- and long-term conservation of Coffea germplasm, respectively. In situ conservation is also an important option for long-term conservation, but there are no genetic reserves specifically set up for conserving Coffea. Protected areas and managed reserves offer the best opportunities for conserving Coffea. A case study for Mascarene Coffea in Mauritius is presented and a strategy for their conservation is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008621028343
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  • 8
    Electronic Resource
    Electronic Resource
    Contrasting genetic diversity relationships are revealed in rice (Oryza sativa L.) using different marker types (1997)
    Springer
    Molecular breeding 3 (1997), S. 115-125 
    Details
    ISSN: 1572-9788
    Keywords: diversity ; genetic mapping ; Oryza sativa ; RAPDs ; rice ; inter-simple sequence repeats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic variation between samples of Oryza sativa from 19 localities in Bangladesh and Bhutan was assessed using two PCR-based molecular marker systems: RAPD (random amplification of polymorphic DNA) and ISSR-PCR (inter-simple sequence repeat polymerase chain reaction). Employing RAPD, a set of 14 decanucleotides of arbitrary sequence directed the amplification of 94 reproducible marker bands, 47 (50%) of which were polymorphic. In addition, a set of 9 ISSR primers were used to direct amplification of 71 PCR products, 40 (56%) of which were polymorphic. Multivariate analyses of the two PCR-based molecular marker data sets provided evidence that the patterns of variation correspond with the classification described by Glaszmann [9] using isozyme analysis. Subtle differences in the relationships revealed between rice groups using the two types of PCR-based marker led to investigations of their map positions using an intraspecific doubled haploid mapping population. The observation that the chromosomal locations of markers can influence diversity assessments is presented and the significance of this is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009635721319
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  • 9
    Electronic Resource
    Electronic Resource
    Contrasting genetic variation amongst lentil landraces from different geographical origins (1998)
    Springer
    Euphytica 102 (1998), S. 265-273 
    Details
    ISSN: 1573-5060
    Keywords: bottleneck ; isozyme ; landrace ; lentil ; RAPD ; South Asia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lentil landraces from South Asia exhibit a low diversity and discordance with landraces from other countries according to a combination of qualitative and quantitative agromorphological characters. They exhibit specific phenological adaptation to the South Asian environment which precludes the direct use of alien germplasm in breeding programs in South Asia. An understanding of the genetic relationships and diversity of South Asian lentil landraces, in relation to landraces from other countries, is important in attempting to widen the genetic base of germplasm in the region. The objectives of this study were to investigate the genetic relationships between lentil landraces from 3 South Asian countries (India, Nepal and Pakistan) and those from 13 other countries and to determine their relative genetic diversities, using both isozyme electrophoresis and random amplified polymorphic DNA (RAPD) analysis. Polymorphisms were observed for 7 isozyme loci (16 alleles) and 22 RAPD loci. According to Nei's genetic distance, germplasm from Afghanistan clustered with that from the South Asian countries. The germplasm from these countries was striking different to that from the other countries studied. Based on genetic distance estimates from RAPD analysis, the countries with the lowest diversity were Pakistan, Afghanistan and Nepal. These data support evidence at the morphological level of a genetic bottleneck in lentil landraces from South Asia. Genetic relationships between countries outside the South Asian group are discussed. Classification into macrosperma and microsperma types did not reflect overall country relationships.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018331432580
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  • 10
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    Differentiation and distribution of indica and japonica rice varieties along the altitude gradients in Yunnan Province of China as revealed by InDel molecular markers (2010)
    Springer
    Details
    Publication Date: 2010-01-29
    Print ISSN: 0925-9864
    Electronic ISSN: 1573-5109
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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