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  • Articles  (928)
  • nitrogen  (376)
  • resistance  (331)
  • Oryza sativa  (222)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (928)
  • 1
    Electronic Resource
    Electronic Resource
    Efficiency of nitrogen-15-labelled fertilizers for rice and rye-grass cultivated in an Ultisol of Brazilian Amazonia (2000)
    Springer
    Biology and fertility of soils 31 (2000), S. 329-333 
    Details
    ISSN: 1432-0789
    Keywords: Key words Acid soil ; Brazilian Amazonia ; N use efficiency ; Priming effect ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The objective of this study was to determine the efficiency of two N fertilizers, (NH4)2SO4 and urea, for rice (Oryza sativa L.) and rye-grass (Lolium multiflorum L.) cultivated in an Ultisol of central Amazonia using 15N as a tracer. Rice was cultivated in the field, while rye-grass was grown in a phytotron. Fertilization with (NH4)2SO4 caused a 16% increase in the yield of rice grains and urea a 36% increase. In both crops total N uptake and N use efficiency of the fertilizers were higher for urea than for (NH4)2SO4. The low values for N derived from fertilizer showed that the fertilizers contributed little to the total N absorbed by the plants. The "priming effect" or positive added N interaction (ANI) between the fertilizer N and soil organic N was observed, especially with urea. Immobilization by soil microorganisms was greater in the presence of urea, while losses were always higher with the (NH4)2SO4 treatments. These losses were significant, and their reduction should allow more efficient use of this N fertilizer. It is possible that the N use efficiency was higher for urea due to a pH increase, caused by urea hydrolysis, which in turn may have favoured the activity of nitrifying bacteria in this extremely acid soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050664
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  • 2
    Electronic Resource
    Electronic Resource
    Fallow residue management effects on upland rice in three agroecological zones of West Africa (2000)
    Springer
    Biology and fertility of soils 31 (2000), S. 501-507 
    Details
    ISSN: 1432-0789
    Keywords: Key words Fallow ; Legumes ; Nitrogen fixation ; Oryza sativa ; Côte d'Ivoire
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Improving fallow quality in upland rice-fallow rotations in West Africa through the site-specific use of leguminous cover crops has been shown to sustain the productivity of such systems. We studied the effects of a range of residue management practices (removal, burning, mulching and incorporation) on fallow biomass and N accumulation, on weed biomass and yield response of upland rice and on changes in soil physical and chemical characteristics in 2-year field trials conducted in three agroecological zones of Côte d'Ivoire. Across fallow management treatments and agroecological zones, rice yields were on average 20–30% higher in legume than in natural fallow plots. Weed biomass was highest in the savanna zone and lowest in the bimodal forest and tended to be less following a legume fallow. Regardless of the type of fallow vegetation and agroecological zone, biomass removal resulted in the lowest rice yields that varied from 0.5 t ha–1 in the derived savanna zone to 1.5 t ha–1 in the Guinea savanna zone. Burning of the fallow vegetation significantly increased yield over residue removal in the derived savanna (0.27 t ha–1, P〈0.05) and bimodal forest zones (0.27 t ha–1, P〈0.01), but not in the Guinea savanna. In both savanna environments, residue incorporation was superior to the farmers' practice of residue removal and rice yield increases were related to amounts of fallow N returned to the soil (r 2=0.803, P〈0.01). In the forest zone, the farmers' practice of residue burning produced the highest yield (1.43 t ha-1 in the case of legumes) and resulted in the lowest weed biomass (0.02 t ha–1). Regardless of the site, improving the quality of the fallow or of its management had no significant effects on either soil physical or soil chemical characteristics after two fallow cycles. We conclude that incorporation of legume residues is a desirable practice for rice-based fallow rotation systems in savanna environments. No promising residue management alternatives to slash-and-burn were apparent for the forest zone. Determining the possible effects on soil productivity will require longer-term experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740000199
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  • 3
    Electronic Resource
    Electronic Resource
    Nitrogen leaching and crop availability in manured catch crop systems in Sweden (2000)
    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 139-152 
    Details
    ISSN: 1573-0867
    Keywords: nitrogen ; N leaching ; liquid manure ; catch crops ; N mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results are presented from five years (1990–1995) of a field leaching experiment on a sandy soil in south-west Sweden. The aim was to study N leaching, change in soil organic N and N mineralization in cropping systems with continuous use of liquid manure (two application rates) and catch crops. N leaching from drains, N uptake in crops and mineral N in the soil were measured. Simulation models were used to calculate the N budget and N mineralization in the soil and to make predictions of improved fertilization strategies in relation to manure applications and changing the time for incorporation of catch crops. In treatments without catch crops, a normal and a double application of manure increased average N leaching by 15 and 34%, respectively, compared to treatment with commercial fertilizer. Catch crops reduced N leaching by, on average, 60% in treatments with a normal application of manure and commercial fertilizer, but only by 35% in the treatment with double the normal application rate of manure. Incorporation of catch crops in spring increased simulated net N mineralization during the crop vegetation period, and also during early autumn. In conclusion, manured systems resulted in larger N leaching than those receiving commercial fertilizer, mainly due to larger applications of mineral N in spring. More careful adaptation of commercial N fertilization with respect to the amounts of NH4-N applied with manure could, according to the simulations, reduce N leaching. Under-sown ryegrass catch crops effectively reduced N leaching in manured systems. Incorporating catch crop residues in late autumn instead of spring might be preferable with respect to N availability in the soil for the next crop, and would not increase N leaching.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009821519042
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  • 4
    Electronic Resource
    Electronic Resource
    Using a Crop/Soil Simulation Model and GIS Techniques to Assess Methane Emissions from Rice Fields in Asia. I. Model Development (2000)
    Springer
    Nutrient cycling in agroecosystems 58 (2000), S. 141-159 
    Details
    ISSN: 1573-0867
    Keywords: methane ; rice ; Oryza sativa ; anaerobic ; model ; simulation ; carbon dynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The development of the MERES (Methane Emissions in Rice EcoSystems) model for simulating methane (CH4) emissions from rice fields is described. The CERES-Rice crop simulation model was used as a basis, employing the existing routines simulating soil organic matter (SOM) decomposition to predict the amount of subsrate available for methanogenesis. This was linked to an existing submodel, described elsewhere in this volume (Arah & Kirk, 2000), which calculates steady-state fluxes and concentrations of CH4 and O2 in flooded soils. Extra routines were also incorporated to simulate the influence of the combined pool of alternative electron acceptors in the soil (i.e., NO3 −, Mn4+, Fe3+, SO4 2−) on CH4 production. The rate of substrate supply is calculated in the SOM routines of the CERES-Rice model from (a) the rate of decomposition of soil organic material including that left from the previous crop and any additions of organic matter, (b) root exudates (modified from the original CERES-Rice model using recent laboratory data), and (c) the decomposition of dead roots from the current crop. A fraction of this rate of substrate supply, determined by the concentration of the oxidized form of the alternative electron acceptor pool, is converted to CO2 by bacteria which outcompete the methanogenic bacteria, thereby suppressing CH4 production. Any remaining fraction of the substrate supply rate is assumed to be potentially available for methanogenesis. The CH4 dynamics submodel uses this potetial methanogenesis rate, along with a description of the root length distribution in the soil profile supplied by the crop model, to calculate the steady-state concentrations and fluxes of O2 and CH4. The reduced form of the alternative electron acceptor pool is allowed to reoxidize when soil pores fill with air if the field is drained. The MERES model was able to explain well the seasonal patterns of CH4 emissions in an experiment involving mid- and end-season drainage and additions of organic material at IRRI in the Philippines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009894619446
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  • 5
    Electronic Resource
    Electronic Resource
    Using a Crop/Soil Simulation Model and GIS Techniques to Assess Methane Emissions from Rice Fields in Asia. II. Model Validation and Sensitivity Analysis (2000)
    Springer
    Nutrient cycling in agroecosystems 58 (2000), S. 161-177 
    Details
    ISSN: 1573-0867
    Keywords: methane ; rice ; Oryza sativa ; anaerobic ; model ; simulation ; carbon dynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The MERES (Methane Emissions from Rice EcoSystems) simulation model was tested using experimental data from IRRI and Maligaya in the Philippines and from Hangzhou in China. There was good agreement between simulated and observed values of total aboveground biomass, root weight, grain yield, and seasonal methane (CH4) emissions. The importance of the contribution of the rice crop to CH4 emissions was highlighted. Rhizodeposition (root exudation and root death) was predicted to contribute about 380 kg C ha−1 of methanogenic substrate over the season, representing 37% of the total methanogenic substrate from all sources when no organic amendments were added. A further 225 kg C ha−1 (22%) was predicted to come from previous crop residues, giving a total of around 60% originating from the rice crop, with the remaining 41% coming from the humic fraction of the soil organic matter (SOM). Sensitivity analysis suggested that the parameter representing transmissivity to gaseous transfer per unit root length (λr) was important in determining seasonal CH4 emissions. As this transmissivity increased, more O2 was able to diffuse to the rhizosphere, so that CH4 production by methanogens was reduced and more CH4 was oxidized by methanotrophs. These effects outweighed the opposing influence of increased rate of transport of CH4 through the plant, so that the overall effect was to reduce the amount of CH4 emitted over the season. Varying the root-shoot ratio of the crop was predicted to have little effect on seasonal emissions, the increased rates of rhizodeposition being counteracted by the increased rates of O2 diffusion to the rhizosphere. Increasing the length of a midseason drainage period reduced CH4 emissions significantly, but periods longer than 6–7 d also decreased rice yields. Organic amendments with low C/N were predicted to be more beneficial, both in terms of enhancing crop yields and reducing CH4 emissions, even when the same amount of C was applied. This was due to higher rates of immobilization of C into microbial biomass, removing it temporarily as a methanogenic substrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009846703516
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  • 6
    Electronic Resource
    Electronic Resource
    Accumulation of mineral elements in tuber periderm of potato cultivars differing in susceptibility to common scab (2000)
    Springer
    Potato research 43 (2000), S. 107-114 
    Details
    ISSN: 1871-4528
    Keywords: actinomycetes ; resistance ; scab incidence ; Solanum tuberosum L. ; Streptomyces scabies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Concentrations of Ca, P, K, Mg, Al, Fe, Mn, Cu and Zn were determined in healthy tuber peelings of cultivars less (Karin, Santé and Symfonia) and more (Agria, Désirée and Tomensa) susceptible to common scab when grown at two sites that differed in the level of scab incidence. The accumulation of some elements was significantly influenced by site, year, cultivar, maturity and the age of tuber periderm. At both sites, Ca and P in periderm tissue declined but Mg increased during the growing season. The Ca/P ratios in tuber periderm of all cultivars greatly decreased 83 days after planting. Concentrations of mineral elements measured at harvest may not reflect conditions present during the infection period, and consequently may not be related to scab incidence or severity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357951
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  • 7
    Electronic Resource
    Electronic Resource
    Incidence of bacteria involved in nitrogen and sulphur cycles in tropical shrimp culture ponds (2000)
    Springer
    Aquaculture international 8 (2000), S. 463-472 
    Details
    ISSN: 1573-143X
    Keywords: nitrogen ; organic matter ; shrimp culture ; sulphur cycle bacteria ; water quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009250004999
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  • 8
    Electronic Resource
    Electronic Resource
    Short-term Cover Crop Decomposition in Organic and Conventional Soils: Characterization of Soil C, N, Microbial and Plant Pathogen Dynamics (2000)
    Springer
    European journal of plant pathology 106 (2000), S. 37-50 
    Details
    ISSN: 1573-8469
    Keywords: carbon ; cellulose ; cover crop ; damping-off ; discriminant analysis ; lignin ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Stages of oat–vetch cover crop decomposition were characterized over time in terms of carbon and nitrogen cycling, microbial activity and damping-off pathogen dynamics in organically and conventionally managed soils in a field and a controlled incubation experiment. A measurement of relative growth consisting of radial growth of a fungal colony over non-sterilized soil divided by that over sterilized soil was used as an assay of suppressiveness. No differences in relative growth of Pythium aphanidermatum and Rhizoctonia solani were detected between organic and conventionally managed soils amended with cover crop residue. Significant effects of cover crop decomposition stage on the relative growth of both pathogens were obtained. Relative growth of P. aphanidermatum was highest just after incorporation and decreased 3 weeks after incorporation. Relative growth of R. solani was highest about 20 days after incorporation, and decreased 2 weeks later in the organic system, but continued to increase in the conventional system. In both experiments, the N or C content, C:N ratio or dry weight of retrieved debris were significantly correlated with relative growth of P. aphanidermatum. Relative growth of R. solani was significantly correlated with the C:N ratio of soil or the C or N content of debris. Microbial activity was not consistently associated with relative growth of either pathogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008720731062
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  • 9
    Electronic Resource
    Electronic Resource
    Characterisation of BaYMV and BaMMV Pathotypes in France (2000)
    Springer
    European journal of plant pathology 106 (2000), S. 365-372 
    Details
    ISSN: 1573-8469
    Keywords: bymovirus ; barley yellow mosaic virus ; barley mild mosaic virus ; resistance ; variability ; ELISA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The reaction of thirty-four barley cultivars from European and Asiatic origin was analysed in six soils infected with barley yellow mosaic virus complex (BaYMV, BaMMV). These soils were selected from 16 sites for their differences in cultivar response. Amongst the six cultivars carrying the ym4 gene (Esterel, Express, Labéa, Majestic, Réjane, Vanoise), only cv Majestic was infected at one site with BaYMV and BaMMV. Concerning BaYMV, three cultivars were infected on all the soils and 19 on none of them. Twelve cultivars were differentially infected depending on the soil. In the case of BaMMV, four cultivars were infected on all the soils and 19 on none of them. Eleven cultivars were differentially infected depending on the soil. ELISA tests revealed the presence, in these soils, of variants of BaYMV and BaMMV that were able to overcome at least seven of the 12 known resistance genes (ym3, ym4, ym6, ym8, ym9, ym10, ym11) and the resistance of three varieties (Tosan Kawa 73, OU1 and Taihoku A) in which the genetic basis is unknown. Amplification by RT-PCR of the N-terminal region for three of BaYMV variants was performed. Nucleotide and amino acid sequences were determined and compared with the corresponding sequence of a common strain of BaYMV-G. A few nucleotide differences were detected between all the French isolates, but there were no strain specific amino acid differences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008710723490
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  • 10
    Electronic Resource
    Electronic Resource
    Physiological regulation of plant-atmosphere ammonia exchange (2000)
    Springer
    Plant and soil 221 (2000), S. 95-102 
    Details
    ISSN: 1573-5036
    Keywords: ammonia exchange ; apoplast ; atmosphere ; glutamine synthetase ; nitrogen ; photorespiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plants have a compensation point for NH3 which ranges from 0.1 to 20 nmol mol-1, and may be several-fold higher or lower than naturally occurring atmospheric NH3 concentrations. This implies that NH3 fluxes over vegetated surfaces are bi-directional and that ammonia exchange with the atmosphere in many cases contributes significantly to the nitrogen economy of vegetation. Physiological regulation of plant–atmosphere NH3 fluxes is mediated via processes involved in nitrogen uptake, transport and metabolism. A rapid turnover of NH3 + in plant leaves leads to the establishment of a finite NH3 + concentration in the leaf apoplastic solution. This concentration determines, together with that of H+, the size of the NH3 compensation point. Barley and oilseed rape plants with access to NH3 + in the root medium have higher apoplastic NH3 + concentrations than plants absorbing NO3 -. Furthermore, the apoplastic NH3 + concentration increases with the external NH3 + concentration. Inhibition of GS leads to a rapid and substantial increase in apoplastic NH3 + and barley mutants with reduced GS activity have higher apoplastic NH3 + than wild-type plants. Increasing rates of photorespiration do not affect the steady-state NH3 + or H+ concentration in tissue or apoplast of oilseed rape, indicating that the NH3 + produced is assimilated efficiently. Nevertheless, NH3 emission increases due to a temperature-mediated displacement of the chemical equilibrium between gaseous and aqueous NH3 in the apoplast. Sugarbeet plants grown with NO3 - seem to be temporarily C-limited in the light due to a repression of respiration. As a consequence, the activity of chloroplastic GS declines during the day causing a major part of NH3 + liberated in photorespiration to be assimilated during darkness when 2-oxoglutarate is supplied in high rates by respiration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004761931558
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  • 11
    Electronic Resource
    Electronic Resource
    The influence of cyst nematodes (Heterodera sacchari) and drought on water relations and growth of upland rice in Côte d'Ivoire (2000)
    Springer
    Plant and soil 220 (2000), S. 235-242 
    Details
    ISSN: 1573-5036
    Keywords: drought ; Heterodera sacchari ; nematode ; Oryza glaberrima ; Oryza sativa ; plant water status ; rice ; West Africa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was undertaken to examine the influence of cyst nematodes (Heterodera sacchari) and drought stress, in isolation and combination, on the water status and growth of rice in Côte d'Ivoire. Drought is considered one of the main yield-limiting factors to upland rice in West Africa, while H. sacchari is emerging as a potentially serious pest. A field study conducted during 1997 at the West Africa Rice Development Association (WARDA) showed that under low water availability in sandy soil, damage to a nematode susceptible Oryza sativa (cv IDSA6) is significant. Visual drought score and physiological parameters were significantly affected (P≤0.01) in plots with a mean population density of 39.4 H. sacchari eggs ml-1 soil at 81 days after sowing, compared to plots with a mean density of 1.2 eggs ml-1. Stomatal conductance, leaf water potential and relative water content were lower, leaf chlorophyll content was greater, and near total yield losses were observed in higher nematode density plots. In 1-l pots following 5 days of imposed drought, the H. sacchari-resistant and drought-resistant O. glaberrima (cv CG14) showed a reduction in stomatal conductance of 73% and in leaf water potential by 0.96 MPa (54%). The effect of H. sacchari (473 eggs l-1 soil) inoculated as cysts to soil before sowing was similar but less severe. The combination of both stresses had similar but yet more pronounced effects than the single stresses on stomatal conductance, leaf water potential and leaf dry weight in CG14. IDSA6 responded in a similar manner to CG14, but less acutely to both stresses. Only the combined stresses resulted in a significant (P≤0.05) reduction in osmotic potential and root dry weight compared with the control, for either CG14 or IDSA6. It is suggested that H. sacchari increased the effects of drought and drought-related losses. This may give a false impression of drought susceptibility in field screening for cultivars, and complicate models which predict crop damage caused by nematodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004734415254
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  • 12
    Electronic Resource
    Electronic Resource
    The effect of three or five years of set-aside on the husbandry and grain yield of subsequent cereal crops in the UK (2000)
    Springer
    Plant and soil 225 (2000), S. 279-297 
    Details
    ISSN: 1573-5036
    Keywords: cereal ; cereal quality ; cereal yields ; natural regeneration ; nitrogen ; nitrogen uptake ; rye-grass ; set-aside ; white clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract During the period 1993–1997, at six contrasting sites located throughout England, two successive cereal test crops were grown both with and without nitrogen fertiliser after three or five years of set-aside or after continuous arable cropping. Vegetation during set-aside included natural regeneration and perennial rye-grass (Lolium perenne) with or without white clover (Trifolium repens), managed by mowing on one or more occasions per year. Establishment of the successive cereal test crops after destruction of the set-aside was generally not a problem. Fertile tiller numbers were increased by inclusion of clover in the set-aside cover or application of inorganic nitrogen. The presence of couch grass (Elytrigia repens) or volunteer cereals in the set-aside covers provided alternative hosts for take-all (Gaeumanomyces graminis) and eyespot (Pseudocercosporella herpotrichoides) and take-all caused some yield reductions in following cereal crops. Management during the set-aside period significantly affected grain yields of the subsequent cereal crops in the majority of the site-year combinations. However, these effects were not as large as would be expected after traditional break crops and were frequently masked by the application of nitrogen fertiliser. Mean yields increased by 80% due to the application nitrogen at the optimum rate compared to nil nitrogen. Most of the effects of set-aside treatment on grain yield were shown to be attributable to soil mineral nitrogen content, but at some sites, infections by take-all or eyespot also accounted for some of the variation. There were no effects of pests that could be related to treatment. The presence of sown clover during the set-aside period had the most consistent effect across sites, affecting tiller populations, grain yield and grain quality of cereal crops. At some sites, establishing a sown cover during the set-aside period, or cutting the cover more than once a year, improved grain yield and quality, and reduced the incidence of some specific weeds and disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026531528082
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  • 13
    Electronic Resource
    Electronic Resource
    Root-shoot interactions in mineral nutrition (2000)
    Springer
    Plant and soil 226 (2000), S. 57-69 
    Details
    ISSN: 1573-5036
    Keywords: ABA ; K/Na selectivity ; nitrate reduction ; nitrogen ; phloem ; phosphate ; root-shoot interactions ; salinity ; xylem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In this paper four classes of co-operative root-shoot interations are addressed. (I) Nitrogen concentrations in the xylem sap originating from the root and in the phloem sap as exported from source leaves are much lower than those required for growth by apices and developing organs. Enrichment of xylem sap N is achieved by xylem to xylem (X-X) transfer, by which reduced N, but not nitrate, is abstracted from the xylem of leaf traces and loaded into xylem vessels serving the shoot apex. Nitrogen enrichment of phloem sap from source leaves is enacted by transfer of reduced N from xylem to phloem (X-P transfer). Quantitative data for the extent of the contribution of X-X and X-P transfer to the nutrition of young organs of Ricinus communis L. and for their change with time are presented. (II) Shoot and root cooperate in nitrate reduction and assimilation. The partitioning of this process between shoot and root is shifted towards the root under conditions of nitrate- and K-deficiency and under salt stress, while P deficiency shifts nitrate reduction almost totally to the shoot. All four changes in partitioning can be attributed to the need for cation-anion balance during xylem transport and the change in electrical charge occurring with nitrate reduction. (III) Even maintenance of the specificity of ion uptake by the root may – in addition to its need for energy – require a shoot-root interaction. This is shown to be needed in the case of the maintenance of K/Na selectivity under the highly adverse condition of salt stress and absence of K supply from the soil. (IV) Hormonal root to shoot interactions are required in the whole plant for sensing mineral imbalances in the soil. This is shown and addressed for conditions of salt stress and of P deficiency, both of which lead to a strong ABA signalling from root to shoot but result in different patterns of response in the shoot.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026431408238
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  • 14
    Electronic Resource
    Electronic Resource
    Effects of glandular trichomes on the development of Phytophthora infestans infection in potato (S. tuberosum) (2000)
    Springer
    Euphytica 114 (2000), S. 165-174 
    Details
    ISSN: 1573-5060
    Keywords: bioassay ; exudates ; inoculation ; late blight ; resistance ; S. berthaultii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract S. berthaulltii Hawkes, a wild potato species, possesses two types of glandular trichomes on its leaf surface: types A and B wich confer resistance to insects. Preliminary results indicated that the presence of glandular trichomes also confers resistance to Phytophthora infestans, the causal agent of late blight. In this paper we report the evaluation of trichome characteristics (density and exudate activities) performed on 8 lines of potato, including the control varieties Desirée and Tropicana, 5 hybrid lines derived from intercrosses and backcrosses between S. tuberosum and S. berthaultii and one accession of S. berthaultii. Whole plants of the 8 genotypes were inoculated with a spore suspension of Phytophthora infestans in order to evaluate their resistance to late blight. The resultant disease incidence correlated negatively with type A trichome density and with polyphenol-oxidase (PPO) activity of type A trichome glands. The removal of type B trichomes exudate by leaf dipping in ethanol did not influence the disease development after artificial inoculation of detached leaves. Type B trichome exudate was extracted and the fraction corresponding to Fatty Acids Sucrose Esters (FASE) was recovered after Thin Layer Chromatography and tested on the fungal mycelium in vitro. The FASE exhibited a slight inhibitory effect on mycelium growth. The results of the experiments are discussed, and several hypotheses regarding the possible role of types A and B trichomes are formulated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003924318577
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  • 15
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    Electronic Resource
    Pathogenicity of the Rhynchosporium secalis population in the Western Cape province of South Africa (2000)
    Springer
    Euphytica 115 (2000), S. 75-82 
    Details
    ISSN: 1573-5060
    Keywords: barley ; resistance ; Rhynchosporium secalis ; scald ; virulence spectrum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The virulence spectra of 50 Rhynchosporium secalis isolates from a population in the Western Cape province of South Africa were determined, and 21 races were detected when evaluated against 17 differential cultivars. The virulence spectrum of the R. secalis population shows considerable variation, and carries unnecessary virulence genes which is quite unexpected, since chiefly susceptible barley cultivars are grown in the south Western Cape. The two most prevalent races, namely races 4 and 7 had three and four virulence genes respectively. Both race 4 and 7 were virulent on the most susceptible cultivars, West China, Steudelli, C.I.8618 and C.I.2226. Considering the resistance genes reported for the cultivars Atlas 46, Turk, and C.I.3515 which showed no susceptible cultivar-pathogen interaction, it would appear that the Rh-Rh3-Rh4 complex is primarily involved in conferring resistance to the local R. secalis isolates
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    Inheritance of resistance to head bugs and its interaction with grain molds in Sorghum bicolor (2000)
    Springer
    Euphytica 112 (2000), S. 167-173 
    Details
    ISSN: 1573-5060
    Keywords: Calocoris angustatus ; grain molds ; head bug ; inheritance ; resistance ; Sorghum bicolor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sorghum head bug, Calocoris angustatus Lethiery is one of the most important pests of grain sorghum in India. Head bug damage increases the severity of grain molds, which renders the grain unfit for human consumption. Therefore, we studied the gene action for resistance to head bugs and grain molds in a diverse array of male-sterile lines and testers in a line × tester mating design under natural infestation. Mean squares for parents, parents vs crosses, lines, testers, and lines × testers were significant for head bug damage and grain mold severity. General combining ability (GCA) effects were significant and negative for ICSA 88019 for head bug damage, and ICSA 88019 and ICSA 88020 for grain molds (except for ICSA 88020 in 1993). General combining ability effects were positive for ICSA 42 and 296 A. GCA effects of lines and testers for head bug damage and grain mold severity were in the same direction (+ve or −ve). Head bug damage in the grain was significantly correlated with grain mold severity. Testers IS 8891, IS 15107, and TAM 2566 (with colored grain and less susceptibility to molds) produced mold-resistant hybrids in combination with all the male-sterile lines, while the reverse was true in the case of Swarna and ICSV 112. Resistance to head bugs showed dominance to partial dominance type of gene action, while in the case of grain molds, it showed dominance to over dominance. Resistance to these pests is governed by both additive and nonadditive types of gene action. The implications of these results are discussed in relation to need for crop improvement in sorghum.
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    URL: http://dx.doi.org/10.1023/A:1003884015765
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    Identification of segregation-distortion-neutral alleles to improve pollen fertility of indica-japonica hybrids in rice (Oryza sativa L.) (2000)
    Springer
    Euphytica 113 (2000), S. 101-107 
    Details
    ISSN: 1573-5060
    Keywords: gamete abortion ; Oryza sativa ; pollen ; wide compatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Utilization of the pronounced heterosis of indica-japonica hybrids in rice had been difficult due to panicle sterility caused by male and female gamete abortion in such hybrids. But the female gamete abortion in the most indica-japonicahybrids has been solved by using an abortion-neutral gene S-5 n called as wide compatibility gene. On the other hand, the problem of pollen sterility remains to be solved for such hybrids. This study was conducted to identify abortion-neutral genes for pollen that may be utilized to alleviate pollen sterility in distant crosses. Some cultivars like Ketan Nangka (KN) and Dular, which are known as wide compatibility variety (WCV), were tested to find whether they possess neutral alleles for segregation distortion of marker genes. The distorted segregation of markers as well as normal segregation were confirmed among progeny lines of three-way crosses, WCV/indica//japonica and japonica//indica/WCV. Neutral alleles for segregation distortion were identified at two gamete gene loci (ga), i.e., ga-14(t) on chromosome 3 and ga-11 on chromosome 7. A new locus ga-14(t) was identified in between Pgi-1 and bc-1 and independent of ga-3 on chromosome 3. The neutral alleles at the two loci may be used to solve pollen sterility in indica-japonica hybrids.
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    URL: http://dx.doi.org/10.1023/A:1003929312252
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    Leaf K/Na ratio predicts salinity induced yield loss in irrigated rice (2000)
    Springer
    Euphytica 113 (2000), S. 109-118 
    Details
    ISSN: 1573-5060
    Keywords: grain yield ; leaf K/Na ratio ; Oryza sativa ; salinity ; screening tools ; yield reduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Salinity is a major constraint to irrigated rice production, particularly in semi-arid and arid climates. Irrigated rice is a well suited crop to controlling and even decreasing soil salinity, but rice is a salt-susceptible crop and yield losses due to salinity can be substantial. The objective of this study was to develop a highly predictive screening tool for the vegetative growth stage of rice to estimate salinity-induced yield losses. Twenty-one rice genotypes were grown over seven seasons in a field trials in Ndiaye, Senegal, between 1991 and 1995 and were subjected to irrigation with moderately saline water (3.5 mS cm-1, electrical conductivity) or irrigation with fresh water. Potassium/sodium ratios of the youngest three leaves (K/NaLeaves) were determined by flame photometry at the late vegetative stage. Grain yield was determined at maturity. All cultivars showed strong log-linear correlations between K/NaLeaves and grain yield, but intercept and slope of those correlations differed between seasons for a given genotype and between genotypes. The K/NaLeaves under salinity was related to grain yield under salinity relative to freshwater controls. There was a highly significant correlation (p 〈 0.001) between K/NaLeaves and salinity-induced grain yield reduction: the most susceptible cultivars had lowest K/NaLeaves and the strongest yield reductions. Although there were major differences in the effects of salinity on crops in both the hot dry season (HDS) and the wet season, the correlation was equally significant across cropping seasons. The earliest possible time to establish the relationship between K/NaLeaves under salinity and grain yield reduction due to salinity was investigated in an additional trial in the HDS 1998. About 60 days after sowing, salinity-induced yield loss could be predicted through K/NaLeaves with a high degree of confidence (p 〈 0.01). A screening system for salinity resistance of rice, particularly in arid and semi-arid climates, is proposed based on the correlation between K/NaLeaves under salinity and salinity-induced yield losses.
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    URL: http://dx.doi.org/10.1023/A:1003981313160
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    Regeneration and genetic transformation of Spanish rice cultivars using mature embryos (2000)
    Springer
    Euphytica 114 (2000), S. 117-122 
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    ISSN: 1573-5060
    Keywords: genetic transformation ; japonica rice cultivar ; mature embryo ; Oryza sativa ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To establish a plant regeneration system from embryogenic callus derived from mature rice embryos, the addition of aminoacids and the effect of two macronutrient solutions MSD and N6D to the basal callus induction medium was tested in three Spanish varieties, Senia, Tebre and Bahia. Aminoacids enhanced the production of embryogenic callus in Tebre and Senia whereas in the case of Bahia, embryogenic callus, which gave rise to a high rate of differentiated shoots, was induced without aminoacids. The macronutrient solution had also to be adjusted for each variety. Pre-regeneration treatment with ABA significantly improved the regeneration rate in all media tested, independently of the media in which the embryogenic callus were induced. In a comparison of growth regulators, BA yielded more shoots than Kin in all varieties whereas the effect of the auxins NAA or IAA was dependent on the variety. Transgenic plants from the three varieties were obtained via an Agrobacterium tumefaciens transformation system, using the optimised culture media.
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    Dynamics of biomass and N accumulation of alfalfa under three N fertilization rates (2000)
    Springer
    Plant and soil 219 (2000), S. 177-185 
    Details
    ISSN: 1573-5036
    Keywords: alfalfa ; growth ; Medicago sativa L. ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The dynamics of biomass and N accumulation following defoliation of alfalfa and the application of N fertilization has rarely been studied under field conditions, particularly in the seeding year. Our objectives were to determine the effect of N fertilization on the dynamics of biomass and N accumulation during the first regrowth of alfalfa in the seeding year, and to determine if a model describing critical N concentration developed for established stands could be used in the seeding year. In two separate experiments conducted in 1992 and 1993, the biomass and N accumulation of alfalfa grown with three N rates (0, 40 and 80 kg N ha-1) were determined weekly. Maximum shoot growth was reached with 40 kg N ha-1 in 1992, and maximum shoot growth was not reached with the highest N fertilization rate in 1993. Nitrogen fixation, root N reserves and soil inorganic N uptake when no N was applied were, therefore, not sufficient to ensure non-limiting N conditions, particularly when growth rates were the highest between 14 to 21 d after defoliation. Nitrogen fertilization increased shoot biomass accumulation in the first 21 d of regrowth, biomass partitioning to the shoots and shoot and taproot N concentrations. The model parameters of critical N concentration developed by Lemaire et al. (1985) for established stands of alfalfa were not adequate in the seeding year. The N requirements per unit of shoot biomass produced are greater in the seeding year than on established stands, and this was attributed to a greater proportion of leaves in the seeding year.
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    Screening the ability of rice roots to overcome the mechanical impedance of wax layers: importance of test conditions and measurement criteria (2000)
    Springer
    Plant and soil 219 (2000), S. 187-196 
    Details
    ISSN: 1573-5036
    Keywords: mechanical impedance ; Oryza sativa ; rice ; roots ; screening ; wax layers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The development of a wax layer method for screening the ability of rice (Oryza sativa L.) roots to overcome mechanical impedance is described. Wax layers (3 mm thick) made of mixtures of white soft paraffin and paraffin wax were installed 50 mm deep in tubes of sand. The sand was watered with nutrient solution and planted with 3-d old rice seedlings. The numbers of root axes per plant that had penetrated the wax layers 24 d after planting were counted. The ratio of penetrated to total root axes per plant gave a misleading measure of root penetration ability, as rice varieties differed in the ratio of penetrated to total axes in a low impedance (3% wax) control. In non-flooded conditions, a 60% wax layer decreased root penetration (number of roots penetrating the wax layer per plant) to a mean of 74% of the low impedance control, whereas an 80% wax layer decreased mean root penetration to 31% of the control. The best measure of root penetration in non-flooded conditions was the number of axes penetrating an 80% wax layer. Flooding decreased root penetration of a 60% wax layer to a mean of 26% of the low impedance control. The best measure of root penetration in flooded conditions was the number of axes penetrating a 60% wax layer.
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    13C NMR study of pine needle decomposition (2000)
    Springer
    Plant and soil 219 (2000), S. 273-278 
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    ISSN: 1573-5036
    Keywords: cellulose ; lignin ; litter ; nitrogen ; Pinus radiata ; tannins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The quality of substrates in plantation forest litter, and their chemistry, can influence decomposition and N cycling. We studied the decomposition of Pinus radiata D. Don needles suspended on branches in windrows, for 3 yr after clear-cutting, using improved solid-state 13C NMR and chemical analysis. The NMR spectra suggested that the concentration of condensed tannins was 12–22%, and showed they were chemically altered during the period 4–12 months after clear-cutting. The spectra showed no evidence for further chemical modification of the tannins during the second or third years. Data for P. radiata needle decomposition in New Zealand indicated rapid loss of mass in the first 3 months, and condensed tannins did not appear to prevent mineralization of C or N. The tannin and lignin concentrations increased with decomposition of the needles, which was consistent with the early mineralization of readily available C compounds.
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    The effects of slash burning on ecosystem nutrients during the land preparation phase of shifting cultivation (2000)
    Springer
    Plant and soil 220 (2000), S. 247-260 
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    ISSN: 1573-5036
    Keywords: cations ; fire ; nitrogen ; nutrients ; phosphorus ; slash-and-burn ; soil ; tropical forests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The most commonly observed change in soil following slash-and-burn clearing of tropical forest is a short-term increase in nutrient availability. Studies of shifting cultivation commonly cite the incorporation of nutrient-rich ash from consumed aboveground biomass into soil as the reason for this change. The effects of soil heating on nutrient availability have been examined only rarely in field studies of slash-and-burn, and soil heating as a mechanism of nutrient release is most often assumed to be of minor importance in the field. Few budgets for above and belowground nutrient flux have been developed in the tropics, and a survey of results from field and laboratory studies indicates that soils are sufficiently heated during most slash-and-burn events, particularly in dry and monsoonal climates, to cause significant, even substantial release of nutrients from non-plant-available into plant-available forms in soil. Conversely, large aboveground losses of nutrients during and after burning often result in low quantities of nutrients that are released to soil. Assessing the biophysical sustainability of an agricultural practice requires detailed information about nutrient flux and loss incurred during management. To this end, current conceptual models of shifting cultivation should be revised to more accurately describe these fluxes and losses.
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    URL: http://dx.doi.org/10.1023/A:1004741125636
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    Soil patchiness in juniper-sagebrush-grass communities of central Oregon (2000)
    Springer
    Plant and soil 223 (2000), S. 47-61 
    Details
    ISSN: 1573-5036
    Keywords: correlograms ; nitrogen ; soil arthropods ; soil resource islands ; variograms ; vesicular-arbuscular mycorrhizae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study compared the sizes, spacings and properties (soil moisture, pH, nitrogen, soil arthropods and VAM) of soil resource islands and bare patches in sagebrush-grass communities invaded by western juniper versus those without juniper. We analyzed 1000 surface soil samples taken from nine 50-m radius circular plots sampled in December of 1991 and May of 1992 on ‘The Island’, one of the few undisturbed areas of sagebrush-grass shrubland in Oregon. Spatial structure was interpreted from correlograms (Moran's I) and standardized semivariograms. The presence of juniper was associated with increased bare area and smaller, more widely spaced grass and sagebrush plants. Soil arthropod numbers and biomass in plots with juniper were only roughly one-fifth of those in sagebrush-grass plots in December. The dominant soil pattern in both sagebrush-grass and juniper-sagebrush-grass plots was regularly-distributed patches spanning a range of sizes and spacings. Plots with juniper had greater patchiness at shorter lags (〈3 m), and patchiness was more developed for soil moisture, net nitrification, and net N mineralization, whereas sagebrush-grass plots had greater patchiness at longer lags (3 – 9 m) and patchiness was more developed for NO3–N, arthropod numbers and biomass. These differences in soil patterns with and without juniper indicate that juniper responds to, or causes, changes in the size of resource islands under sage and grass when it invades sage-grass communities.
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    URL: http://dx.doi.org/10.1023/A:1004745329332
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    Estimating crop N uptake from organic residues using a new approach to the 15N isotope dilution technique (2000)
    Springer
    Plant and soil 223 (2000), S. 33-46 
    Details
    ISSN: 1573-5036
    Keywords: crop residues ; nitrogen ; organic residues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Experiments were conducted to test a new approach to the 15N isotope dilution technique for estimating crop N uptake from organic inputs. Soils were pre-labelled with 15N fertiliser and a carbon source. These were then incubated until there was stabilisation of the 15N abundance of the inorganic N pool and resumption of inorganic N concentrations. Residues were then applied to the soils and planted with ryegrass (Lolium perenneL.) to determine the nitrogen derived from the residue (Ndfr) using the isotope dilution equations. This method was compared with the direct method, i.e. where 15N-labelled residues were added to the soil and Ndfr in the ryegrass calculated directly. Estimates of percentage nitrogen derived from the residue (%Ndfr) alfalfa (Medicago sativaL.) in the ryegrass, were similar, 22 and 23% for the direct and soil pre-labelling methods, respectively, in the Wechsel sandy loam. Also, estimates of the %Ndfr from soybean (Glycine max (L.) Merr) residues in the Krumbach sandy loam were similar 34% (direct) and 36% (soil pre-labelling approach). However, in the Seibersdorf clay loam, the %Ndfr from soybean was 49% using the direct method and 61% using the soil pre-labelling method; yet Ndfr from common bean residue was 46% using the direct approach and 40% using the pre-labelling, not significantly different (P 〉 0.05). The soil pre-labelling approach appears to give realistic values for Ndfr. It was not possible to obtain an estimate of Ndfr using the soil pre-labelling method from the maize residues (Zea mays L.) in two of the soils, as there was no increase in the total N of the ryegrass over the growing period. This was probably due to microbial immobilisation of inorganic N, as a result of the wide C:N ratio of the residue added. The results suggest that the new soil pre-labelling method is feasible and that it is a potentially useful technique for measuring N release from a wide range or organic residues, but it requires further field-testing.
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    Chemistry and toughness predict leaf litter decomposition rates over a wide spectrum of functional types and taxa in central Argentina (2000)
    Springer
    Plant and soil 218 (2000), S. 21-30 
    Details
    ISSN: 1573-5036
    Keywords: carbon ; functional types ; leaf tensile strength ; litter quality ; mass loss ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Litter decomposition, a major determinant of ecosystem functioning, is strongly influenced by the litter quality of different species. We aimed at (1) relating interspecific variation in leaf litter decomposition rate to the functional types different species belong to; and (2) understanding the chemical and/or physical basis for such variation and its robustness to environmental factors. We selected 52 Angiosperms from a climatic gradient in central-western Argentina, representing the widest range of functional types and habitats published so far. Ten litter samples of each species were simultaneously buried for 9 weeks during the 1996 summer in an experimental decomposition bed. Decomposition rate was defined as the percentage of dry mass loss after incubation. Chemical litter quality was measured as carbon (C) content, nitrogen (N) content, and C-to-N ratio. Since tensile strength of litter and living leaves were strongly correlated, the latter was chosen as an indicator of physical litter quality. A subset of 15 species representing different functional types was also incubated in England for 15 weeks, following a similar experimental procedure. Litter C-to-N and leaf tensile strength of the leaves showed the strongest negative associations with decomposition rate, both at the species and at the functional-type level. Decomposition rates of the same species in Argentina and in England were strongly correlated. This reinforces previous evidence that species rankings in terms of litter decomposition rates are robust to methodological and environmental factors. This paper has shown new evidence of plant control over the turnover of organic matter through litter quality, and confirms, over a broad spectrum of functional types, general models of resource allocation. The strong correlations between leaf tensile strength – a trait that is easy and quick to measure in a large number of species – decomposition rate, and C-to-N ratio indicate that leaf tensile strength can be useful in linking plant quality to decomposition patterns at the ecosystem level.
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    Cd2+ effects on transmembrane electrical potential difference, respiration and membrane permeability of rice (Oryza sativa L) roots. (2000)
    Springer
    Plant and soil 219 (2000), S. 21-28 
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    ISSN: 1573-5036
    Keywords: cadmium ; membrane permeability ; Oryza sativa ; respiration ; transmembrane electrical potential difference (Em)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Among other detrimental effects of the heavy metal Cd2+, a decrease in the plant content of essential mineral nutrients is known. In this study, the effect of Cd2+ on different physiological activities of rice roots involved in nutrient acquisition has been studied. Upon addition of 0.1 or 1 mM Cd2+ to the experimental solution, root cell membranes depolarized in few minutes, reaching very low Em values. This effect was transient and the initial membrane potential recovered totally within 6–8 h. Only the highest concentration used had an inhibitory effect on root respiration. Significant respiratory inhibition appeared after 2 h of exposure to Cd2+ and lasted for at least 4 h. In turn, membrane permeability increased in the presence of Cd2+ for at least 8 h, inducing K+ efflux from the roots. The relationship between these parameters and their possible involvement in lowered nutrient content in Cd2+-treated plants is discussed.
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    On variation in forest floor thickness across four red pine plantations in Pennsylvania, USA (2000)
    Springer
    Plant and soil 219 (2000), S. 57-69 
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    ISSN: 1573-5036
    Keywords: decomposition ; litter quality ; mycorrhiza ; nitrogen ; phosphorus ; saprotrophic microorganisms ; tannins ; forest soils ; acidification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We studied variation in forest floor thickness in four plantations of red pine (Pinus resinosa Ait.) which were similar in age, soil type and associated vegetation. The plantations were located (west to east) in the Clear Creek, Moshannon, Sproul and Tiadaghton State Forests of Pennsylvania, USA. A gradient in forest floor thickness exists across these plantations; the forest floor is thickest in the west and it becomes progressively thinner toward the east. Decomposition of imported litter increased from west to east, suggesting that the variation in forest floor thickness is related to variation in the rate of decomposition. Decomposition rates were related to saprotroph abundance. Variation in forest floor N and phenolic concentrations, in overall mycorrhiza density and in the relative proportions of three common mycorrhiza morphotypes could not explain the variation in decomposition rate. The P concentrations and pH of the forest floor were significantly lower at Clear Creek and Moshannon, where decomposition rates were lowest, compared to Sproul and Tiadaghton, where decomposition rates were most rapid. This suggests that P concentration and pH may have exerted some control on decomposition.
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    URL: http://dx.doi.org/10.1023/A:1004748220856
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    Dynamics of mineral N availability in grassland ecosystems under increased [CO2]: hypotheses evaluated using the Hurley Pasture Model (2000)
    Springer
    Plant and soil 224 (2000), S. 153-170 
    Details
    ISSN: 1573-5036
    Keywords: climate change ; immobilisation ; mineralisation ; N-turnover ; nitrogen ; soil organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The following arguments are outlined and then illustrated by the response of the Hurley Pasture Model to [CO2] doubling in the climate of southern Britain. 1. The growth of N-limited vegetation is determined by the concentration of N in the soil mineral N pools and high turnover rates of these pools (i.e., large input and output fluxes) contribute positively to growth. 2. The size and turnover rates of the soil mineral N pools are determined overwhelmingly by N cycling into all forms of organic matter (plants, animals, soil biomass and soil organic matter — `immobilisation' in a broad sense) and back again by mineralisation. Annual system N gains (by N2 fixation and atmospheric deposition) and losses (by leaching, volatilisation, nitrification and denitrification) are small by comparison. 3. Elevated [CO2] enriches the organic matter in plants and soils with C, which leads directly to increased removal of N from the soil mineral N pools into plant biomass, soil biomass and soil organic matter (SOM). ‘Immobilisation’ in the broad sense then exceeds mineralisation. This is a transient state and as long as it exists the soil mineral N pools are depleted, N gaseous and leaching losses are reduced and the ecosystem gains N. Thus, net immobilisation gradually increases the N status of the ecosystem. 4. At the same time, elevated [CO2] increases symbiotic and non-symbiotic N2 fixation. Thus, more N is gained each year as well as less lost. Effectively, the extra C fixed in elevated [CO2] is used to capture and retain more N and so the N cycle tracks the C cycle. 5. However, the amount of extra N fixed and retained by the ecosystem each year will always be small (ca. 5–10 kg N ha-1 yr-1) compared with amount of N in the immobilisation-mineralisation cycle (ca. 1000 kg N ha-1 yr-1). Consequently, the ecosystem can take decades to centuries to gear up to a new equilibrium higher-N state. 6. The extent and timescale of the depletion of the mineral N pools in elevated [CO2] depends on the N status of the system and the magnitude of the overall system N gains and losses. Small changes in the large immobilisation—mineralisation cycle have large effects on the small mineral N pools. Consequently, it is possible to obtain a variety of growth responses within 1–10 year experiments. Ironically, ecosystem models — artificial constructs — may be the best or only way of determining what is happening in the real world.
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    Autumn leaf colours as indicators of decomposition rate in sycamore (Acer pseudoplatanus L.) (2000)
    Springer
    Plant and soil 225 (2000), S. 33-38 
    Details
    ISSN: 1573-5036
    Keywords: deciduous tree ; foliar pigmentation ; fungus ; litter mass loss ; nitrogen ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We tested the hypothesis that there is a causal connection between autumn colour, nutrient concentration and decomposibility of fresh leaf litter. Samples from patches of different autumn colours within the leaves of the deciduous tree sycamore (Acer pseudoplatanus) were sealed into litter bags and incubated for one winter in an outdoor leaf mould bed. Green leaf patches were decomposed faster than yellow or brown patches and this corresponded with the higher N and P concentrations in the former. Black patches, indicating colonisation by the tar spot fungus Rhytisma acerinum, were particularly high in P, but were decomposed very slowly, owing probably to resource immobilisation by the fungus. The results supported the hypothesis and were consistent with a previous study reporting an interspecific link between autumn coloration and decomposition rate. Autumn leaf colour of deciduous woody plants may serve as a useful predictor of litter decomposibility in ecosystem or biome scale studies where extensive direct measurements of litter chemistry and decomposition are not feasible.
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    URL: http://dx.doi.org/10.1023/A:1026530214258
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    Improvement of resistance to Fusarium head blight by recurrent selection in an intermating breeding spring wheat population using the dominant male-sterile gene ms 2 (2000)
    Springer
    Euphytica 112 (2000), S. 79-88 
    Details
    ISSN: 1573-5060
    Keywords: Fusarium head blight ; intermating breedingpopulation ; male-sterile gene ms 2 ; recurrent selection ; resistance ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Four cycles of recurrent selection for FHB resistance were conducted in an intermating wheat breeding population using the dominant male-sterile gene ms 2 during 1987–1991.Five cycles of phenotypic mass selection for male-sterile plants were evaluated using the soil-surface inoculation method in Experiment I. Experiment II evaluated changes in FHB scores during five cycles of progeny selection for fertile plants using the single-floret inoculation method. In Experiment I, the average level of FHB response increased to MR level in C4, compared to MS level in C0. The numbers of infected spikelets and diseased kernels decreased 0.32 and 2.68 per cycle, respectively. In Experiment II, the average level of FHB response increased to R level in C4F1. The numbers of infected spikelets and diseased kernels decreased 0.93 and 4.58 per cycle, respectively. In both experiments, the largest selection gains were realized in the first cycle. The frequencies of R and MR individuals were increased significantly. The frequencies of individuals with FHB response equal and/or superior to Sumai 3 were increased to 5–8% in C4 and 25% in C4F1after the fourth cycle. Agronomic traits tended to be slightly improved in selected populations. Compared to 2% in C0, about 34% of lines superior in both FHB resistance and agronomic traits in C4F1 were selected to enter the conventional breeding program for further evaluation. Sixty three semidwarf lines superior in both FHB resistance and yield potential were selected from the F5 generations derived from C1F1 to C4F1. From them, two resistant cultivars with high-yielding potential were developed and commercialized in the Lower Yangtze Valley. Recurrent selection appears to be highly effective and feasible in shifting the average FHB response of the intermating population in the desirable direction, thereby enhancing the frequency of resistant individuals.
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    URL: http://dx.doi.org/10.1023/A:1003850123088
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    Stable resistance in barley to Pyrenophora teres f. teres isolates from the Nordic-Baltic region after increase on standard host genotypes (2000)
    Springer
    Euphytica 113 (2000), S. 71-77 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; net blotch ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results from tests of a mixture of Finnish net blotch, Pyrenophora teres Drechs. f. teres Smedeg., isolates on a differential series of barley seedlings, comprising 17genotypes, indicated that patterns of infection response (IR)and percentage leaf area damaged (PLAD) were unaffected by differences in seedling size. Variation of the concentration of inoculum between 1,250 conidia ml-1 and 20,000 conidia ml-1 produced similar patterns of IR and PLAD on the differential series. IR and PLAD scored on the second seedling leaf differentiated resistance to P. teres f. teresamong the genotypes better than on the first seedling leaf. Ina second experiment, 120 single-spore P. teres f. teres isolates from Finland, Sweden, Norway, Latvia, Estonia and Ireland were used in tests conducted in the greenhouse to differentiate them in terms of virulence reaction on seedlings of six differential barley genotypes. Each isolate was tested directly following isolation from the leaf material and after having passaged each through barley cvs. Arve or Pohto, to produce 360 isolates in total. Virulence of the isolates differed significantly on the members of the differential series, but differences associated with country of origin and passaging, and interactions, were small. It is concluded that little variation between virulence of P. teres f. teres isolates is evident over a large geographic area, incorporating Nordic and Baltic countries, and Ireland. Barley genotype response to P. teres f. teres appeared to be of more significance than relative virulence of the pathogen isolates. This could simplify breeding barley for improved resistance to this phytopathogen.
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    URL: http://dx.doi.org/10.1023/A:1003912825455
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    Genetic study of Coffea canephora coffee tree resistance to Meloidogyne incognita nematodes in Guatemala and Meloidogyne sp. nematodes in El Salvador for selection of rootstock varieties in Central America (2000)
    Springer
    Euphytica 113 (2000), S. 79-86 
    Details
    ISSN: 1573-5060
    Keywords: Coffea canephora ; Meloidogyne incognita ; Meloidogyne sp ; resistance ; rootstock varieties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Root-knot nematodes, Meloidogyne incognita in Guatemala and Meloidogyne sp. in El Salvador frequently cause very serious damage to Coffea arabica. Hypocotyledon grafting on C. canephoravar. Robusta is practiced on a very wide scale to control these pests. However, rootstock seeds come from non selected trees which provide 30–40%resistance. In this article, we examine the possibility of improving resistance to M. incognita and Meloidogyne sp. Hybrids were created using two North Carolina II factorial mating designs and tested under controlled conditions for resistance to isolates of the two nematodes. In the trial with Meloidogyne sp. the number of nematodes per plant was counted, and in the trial withM. incognita a root damage index was established. Vegetative measurements (height, weight of aerial parts and roots) were taken in both trials. The parents were classed according to their cross value and genetic variance was estimated. In the factorial trial conducted with the Meloidogynesp. isolate from El Salvador, parents T3561 and T3751transmitted high resistance levels of 56 and 54%,respectively, to their progenies, as opposed to 9% for the other parents. The cross between those two parents achieved 78% resistant plants. In the trial with the M. incognita isolate from Guatemala, similar results were found. The same two parents transmitted resistance to 64% of their progenies, as opposed to 36% for the other parents. Classification of the parents did not differ from one trial to the other. The existence of a complex nematode resistance locus in the C. canephora species seems highly likely. The results show that it is possible to select rootstock varieties that are more resistant to the main Meloidogyne nematodes in Guatemala and El Salvador. Given the average heritability values (0.28–0.30) and the possibility of applying strong selection intensity, the genetic progress expected in the next selection cycle should be substantial.
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    URL: http://dx.doi.org/10.1023/A:1003931918187
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    Genetics of resistance to early blight (Alternaria solani Sorauer) in tomato (Lycopersicon esculentum L.) (2000)
    Springer
    Euphytica 113 (2000), S. 187-193 
    Details
    ISSN: 1573-5060
    Keywords: epistasis ; Lycopersicon esculentum ; Lycopersicon pimpinellifolium ; resistance ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic nature of early blight resistance in tomato was studied in three crosses at seedling and adult plant stages. A six generation mean analysis of the cross Arka Saurabh (susceptible) × IHR1939 (resistance) and its reciprocal cross revealed that the resistance to early blight was conferred by recessive polygenes at both seedling and adult plant stages. This polygenic early blight resistance revealed the importance of additive and additive × additive gene effects at seedling stage and higher magnitude of dominance and dominance× dominance gene effects at adult plant stage. Evaluation of parents, F1, F2 and backcross generations of IHR1816 (resistance) × IHR1939 (resistance) revealed that the early blight resistance genes in IHR1816 (Lycopersicon esculentum NCEBR-1) and IHR1939 (Lycopersicon pimpinellifolium L4394) are independent.
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    URL: http://dx.doi.org/10.1023/A:1003929303632
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    Isolation of mildew resistant wheat-rye translocation lines from a double substitution line (2000)
    Springer
    Euphytica 115 (2000), S. 167-172 
    Details
    ISSN: 1573-5060
    Keywords: alien genetic variation ; chromosome translocation ; powdery mildew ; resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A powdery mildew resistant double disomic wheat-rye substitution line carrying rye chromosomes 1R and 2R was crossed with normal bread wheats. The F2 generation was analysed cytologically by C-banding. Wheat-rye chromosome translocations involving both rye chromosomes 1R and 2R were frequent in F2. Lines with translocations of 1R and 2R were harvested separately. After four generations of selfing and selection for mildew resistance and fertility, fully fertile resistant lines were selected and analysed cytologically. Lines with 1BL/1RS and 2BS/2RL translocations were identified. The resistance on chromosome 1RS could not be shown to be different from control varieties carrying the same rye segment, while the resistance on 2RL is much broader than the earlier known 2RL derived resistance in the line Transec.
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    URL: http://dx.doi.org/10.1023/A:1004018500970
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    Ammonium in relation to proline accumulation in detached rice leaves (2000)
    Springer
    Plant growth regulation 30 (2000), S. 139-144 
    Details
    ISSN: 1573-5087
    Keywords: ammonium ; methionine sulfoximine ; Oryza sativa ; proline accumulation ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ammonium accumulation in relation to prolineaccumulation in detached rice leaves under stressconditions was investigated. Ammonium accumulation indark-treated detached rice leaves preceded prolineaccumulation. Ammonium accumulation caused by waterstress coincided closely with proline accumulation indetached rice leaves. Exogenous NH4Cl andmethionine sulfoximine (MSO), which caused anaccumulation of ammonium in detached rice leaves,increased proline content. It was found that prolinein NH4Cl- or MSO-treated rice leaves is lessutilized than in water-treated rice leaves (controls). These results are in agreement with the observationthat a decrease in proline utilization contributes tothe accumulation of proline in dark-treated and waterstressed rice leaves. Although ammonium contentincreased in Cd- and Cu-treated rice leaves, theincrease in ammonium content was only observed afterthe increase in proline content.
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    URL: http://dx.doi.org/10.1023/A:1006329919243
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    Sources of resistance to downy mildew in narbon (Vicia narbonensis) and common (Vicia sativa) vetches (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 153-156 
    Details
    ISSN: 1573-5109
    Keywords: common vetch ; downy mildew ; narbon vetch ; Peronospora viciae ; resistance ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Downy mildew (Peronospora viciae (Berk) de Bary) is an important disease of vetches (Vicia spp.) in the Mediterranean Region. Narbon and common vetch germplasm accessions originating from different countries, and advanced breeding narbon vetch genotypes, were evaluated for reaction to downy mildew for two seasons under field conditions. In narbon vetch, there were resistant sources from both germplasm accessions and advanced genotypes. From the germplasm accessions, 25 accessions were resistant while from the advanced breeding genotypes, 10 had resistance, with 2–3 ratings. In common vetch, many accessions were highly resistant to downy mildew. These were widely distributed in different countries, mainly Turkey, Italy, Syria and Iran. In general, common vetch had higher sources of resistance than the narbon vetch accessions. These new sources of resistance to downy mildew will be incorporated into the forage legume improvement project in a continuous effort to identify cultivars suitable for replacing fallow in the cereal-based cropping systems.
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    URL: http://dx.doi.org/10.1023/A:1008766109492
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    Variation in resistance to the cabbage aphid ( Brevicoryne brassicae) between and within wild and cultivated Brassica species (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 395-401 
    Details
    ISSN: 1573-5109
    Keywords: Brassica ; Brevicoryne brassicae ; resistance ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seven Brassica species were evaluated for their resistance to the cabbage aphid, Brevicoryne brassicae, in a series of field experiments. Four wild Brassica species, two 8 chromosome species with similarities to the B genome of Brassica nigra (Brassica fruticulosa and Brassica spinescens) and two 9 chromosome species containing the C genome (Brassica incana and Brassica villosa) were identified as possessing consistently high levels of antibiosis mediated resistance to B. brassicae. None of the species were shown to possess consistently high levels of antixenosis resistance. In more detailed glasshouse experiments one B-like genome species, B. fruticulosa, showed considerable variation between accessions collected from different sites for resistance to B. brassicae. In addition, individual accessions of one A genome species (Brassica rapa) and one C genome species (Brassica alboglabra) were shown to be highly variable in their resistance to B. brassicae, some plants of each accession being highly resistant and others very susceptible. The implications of the variability in resistance to B. brassicae within wild Brassica species for exploitation in Brassica breeding programmes are discussed.
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    URL: http://dx.doi.org/10.1023/A:1008755411053
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    Effect of NaCl stress on H2O2 metabolism in rice leaves (2000)
    Springer
    Plant growth regulation 30 (2000), S. 151-155 
    Details
    ISSN: 1573-5087
    Keywords: ascorbate peroxidase ; catalase ; glutathione reductase ; glycolate oxidase ; hydrogen peroxide ; Oryza sativa ; peroxidase ; superoxide dismutase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of NaCl stress on H2O2 metabolismin detached rice leaves was studied. NaCl (200 mM)treatment did not cause the accumulation ofH2O2 and resulted in no increase in lipidperoxidation and membrane leakage of leaf tissues. The activities of peroxidase, ascorbate peroxidase,superoxide dismutase, and glutathione reductase wereobserved to be greater in NaCl-stressed rice leavesthan in control leaves. However, glycolate oxidasewas lower in NaCl-treated rice leaves than in thecontrol leaves. There was no difference in catalaseactivity between NaCl and control treatments. Theseresults suggest that some antioxidant enzymes can beactivated in response to oxidative stress induced byNaCl.
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    URL: http://dx.doi.org/10.1023/A:1006345126589
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    Effects of excreta return on properties of a grazed pasture soil (2000)
    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 79-85 
    Details
    ISSN: 1573-0867
    Keywords: exchangeable cations ; nitrogen ; soil carbon ; soil quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The long term impact of excreta return on some chemical and biological properties of a pasture soil fertilised with sulphur and phosphate was studied in a system that had been with or without excreta for 23 years. Excreta free areas that had developed under electric fencelines, and parallel transects in the paddocks, were sampled to provide this comparison. Sampling was to 300 mm depth in 0–75, 75–150 and 150–300 mm sections. Total carbon and nitrogen were 20% higher in the 0–150 mm soil layer of areas receiving excreta but did not differ in the 150–300 mm layer. Carbon:nitrogen ratios were similar in both systems as was mineralisable nitrogen, both absolutely and as a percentage of total in the 0–75 mm layer. Significantly more N was mineralised in the 75–150 mm layers of the areas receiving excreta but this was reversed in the 150–300 mm layer. Nitrification rate was higher in all layers of the excreta areas. Inorganic and organic P fractions did not differ significantly. Total P was significantly higher in the 0–75 mm layer and significantly lower in the 150–300 mm layer of the excreta areas. Exchangeable potassium was much higher throughout the excreta areas while this was offset by calcium. The sum of the cations was similar in both areas. Excreta affected most of the diagnostic soil tests used for fertiliser recommendations. The soil properties measured did not reflect clearly the differences in productivity that were obvious in the two areas. It is concluded that excreta return has a impact resulting in increased organic matter storage. Short-term effects of urine have a greater impact on productivity. The major effect is on the disposition of cations and available P.
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    URL: http://dx.doi.org/10.1023/A:1009842727493
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    Nitrogen in percolation water in paddy fields with a rice/wheat rotation (2000)
    Springer
    Nutrient cycling in agroecosystems 57 (2000), S. 75-82 
    Details
    ISSN: 1573-0867
    Keywords: nitrogen ; leaching ; paddy soil ; wheat ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen in percolation water was observed in paddy field soil under rice/wheat rotation. Different N-application rates were designed. Porous pipes were installed in triplicate at depths of 30, 60 and 90 cm to collect the water in the period of wheat growth. Suction cups were installed in triplicate at the same depths to collect the water during the period of rice growth. NH4 +, NO3 - and total N in the water were analysed with a continuous-flow nitrogen analyzer. Results showed that nitrate was the predominant form of nitrogen in percolation water during the period of wheat growth. Nitrate leaching was high in early spring after the `tillering fertilisation'. More than 50 mg l-1 of nitrate concentration in percolation water was observed for 30 and 60 cm in depth and more than 15 mg l-1 were observed for 90 cm. The concentration decreased quickly and was very low, less than 2 mg l-1 usually, in the earring stage of wheat. Nitrate in water was low, less than 1.5 mg l-1 usually, when the field was flooded during the period of rice growth. Some soluble organic N existed in the water. Nitrate in percolation water increased when the field was drained. The leaching loss of nitrogen during winter wheat growth period was estimated to be about 3.4% of the N-fertiliser applied at the normal application rate of farmers; for the rice growth period it was around 1.8%. Although a reduced N-application decreased N leaching, it caused a marked decrease in crop yield.
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    Nitrogen (N) management in the ‘De Marke’ dairy farming system (2000)
    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 231-240 
    Details
    ISSN: 1573-0867
    Keywords: nitrogen ; N ; nitrate ; ammonia ; dairy farming ; systems research ; environment ; sandy soils ; groundwater ; leaching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the sandy regions of The Netherlands, high losses of N from intensified dairy farms are threatening the environment. Therefore, government defined decreasing maximum levy-free N surplusses for the period 1998–2008. On most dairy farms, the current N surplus has to be reduced by half at least. Farmers fear that realizing these surplusses will be expensive, because it limits application of animal manure, which then has to be exported or additional land has to be bought. Moreover, farmers are worried about the impact on soil fertility. To explore the possibilities for reducing surplusses of average intensive farms by improved nutrient management, farming systems research is carried out at prototype farm ‘De Marke’. Results are compared with results of a commercial farm in the mid-1980s, the moment that systems research started and introduction of the milk quota system put a halt to further intensification. Results indicate that average intensive farms can realise a reduction in N surplus to a level below the defined final maximum, without the need to buy land or to export slurry. Inputs of N in purchased feeds and fertilisers decreased by 56 and 78%, respectively. Important factors are reduced feed intake per unit milk, as a result of a higher milk yield per cow, less young stock and judicious feeding, an improved utilization of ‘home-made’ manure and a considered balance between the grassland and maize area. Changed soil fertility status did not constrain crop production. Nitrate concentration in the upper groundwater decreased from 200 to 50 mg l-1, within a few years.
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    Transport of dissolved inorganic nitrogen from the major rivers to estuaries in China (2000)
    Springer
    Nutrient cycling in agroecosystems 57 (2000), S. 13-22 
    Details
    ISSN: 1573-0867
    Keywords: biogeochemistry ; estuary ; nitrogen ; river ; transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Long-term results of the monthly measurement of dissolved inorganic nitrogen (DIN) concentrations in three major rivers in China are presented. These data are combined with river discharge data to calculate the DIN loads discharged into the ocean. About 774.90 × 103, 55.38 × 103and 144.55 × 103tons of DIN were transported to their respective estuaries each year by the Changjiang, the Huanghe and the Zhujiang in 1980–1989, mainly in the form of nitrate (〉 80 percent). The annual transport of DIN and mean concentration of nitrate in the Changjiang had increased drastically (four-fold) in the last 29 years, especially during the 1980s. Although nitrate concentrations of the Zhujiang and the Huanghe had also increased in the 1980s, their total annual loads of DIN varied mainly with annual runoff volumes, showing no obvious uptrends. Our results also demonstrate that the majority of the DIN load of each river was transported in the high-flow period (70–80 percent). A positive relationship is observed between the annual DIN transport of the Changjiang and the annual application of chemical fertilizers in its catchment. The annual DIN loads of the Huanghe and the Zhujiang were influenced mainly by runoff volume, and also by application of chemical fertilizers.
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    Nitrogen mobilization in Asia (2000)
    Springer
    Nutrient cycling in agroecosystems 57 (2000), S. 1-12 
    Details
    ISSN: 1573-0867
    Keywords: Asia ; fertilizer ; ammonia ; food ; greenhouse effect ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The addition of anthropogenic N by food and energy production to the global environment contributes to the greenhouse effect, acid deposition, photochemical smog, stratospheric ozone depletion and eutrophication of fresh and marine waters. On a global basis, anthropogenic N mobilization is greater then natural sources of bio-reactive N. Currently, Asia is a hotspot of N mobilization and distribution to downwind and downstream environments, primarily due to food production. Asia's contribution will continue to increase, not only due to population growth, but also to increases in the per capita consumption of food and energy. This paper provides an overview of the global N cycle, presents an analysis of N dynamics within agroecosystems, examines N mobilization in Asia and discusses possible rates of N mobilization in the future.
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    URL: http://dx.doi.org/10.1023/A:1009832221034
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    Pruning effects on root distribution and nutrient dynamics in an acacia hedgerow planting in northern Kenya (2000)
    Springer
    Agroforestry systems 50 (2000), S. 59-75 
    Details
    ISSN: 1572-9680
    Keywords: below-ground competition ; carbohydrates ; nitrogen ; nutrient leaching ; resin cores
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tree pruning is a common management practice in agroforestry for mulching and reducing competition between the annual and perennial crop. The below-ground effects of pruning, however, are poorly understood. Therefore, nutrient dynamics and root distribution were assessed in hedgerow plantings of Acacia saligna (Labill.) H.L. Wendl. after tree pruning. Pruning to a height of 1.5 m was carried out in March and September 1996. In July and October 1996, the fine root distribution (〈 2 mm) and their carbohydrate contents were determined at three distances to the tree row by soil coring. At the same time, foliar nutrient contents were assessed, whereas nutrient leaching was measured continuously. The highest root length density (RLD) was always found in the topsoil (0–0.15 m) directly under the hedgerow (0–0.25 m distance to trees). Pruning diminished the RLD in the acacia plots at all depths and positions. The relative vertical distribution of total roots did not differ between trees with or without pruning, but live root abundance in the subsoil was comparatively lower when trees were pruned than without pruning. In the dry season, the proportion of dead roots of pruned acacias was higher than of unpruned ones, while the fine roots of unpruned trees contained more glucose than those of pruned trees. Pruning effectively reduced root development and may decrease potential below-ground competition with intercropped plants, but the reduction in subsoil roots also increased the danger of nutrient losses by leaching. Leaching losses of such mobile nutrients as NO3− were likely to occur especially in the alley between pruned hedgerows and tended to be higher after pruning. The reduced size of the root system of pruned acacias negatively affected their P and Mn nutrition. Pruning also reduced the function of the trees as a safety net against the leaching of nutrients for both NO3− and Mn, though not for other studied elements. If nutrient capture is an important aim of an agroforestry system, the concept of alley cropping with pruning should be revised for a more efficient nutrient recycling in the system described here.
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    Tithonia diversifolia as a green manure for soil fertility improvement in western Kenya: A review (2000)
    Springer
    Agroforestry systems 49 (2000), S. 201-221 
    Details
    ISSN: 1572-9680
    Keywords: biomass transfer ; integrated nutrient management ; nitrogen ; nutrient cycling ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tithonia diversifolia, a shrub in the family Asteraceae, is widely distributed along farm boundaries in the humid and subhumid tropics of Africa. Green biomass of tithonia has been recognized as an effective source of nutrients for lowland rice (Oryza sativa) in Asia and more recently for maize (Zea mays) and vegetables in eastern and southern Africa. This paper reviews the potential of tithonia green biomass for soil fertility improvement based on recent research in western Kenya. Green leaf biomass of tithonia is high in nutrients, averaging about 3.5% N, 0.37% P and 4.1% K on a dry matter basis. Boundary hedges of sole tithonia can produce about 1 kg biomass (tender stems + leaves) m−1 yr−1 on a dry weight basis. Tithonia biomass decomposes rapidly after application to soil, and incorporated biomass can be an effective source of N, P and K for crops. In some cases, maize yields were even higher with incorporation of tithonia biomass than with commercial mineral fertilizer at equivalent rates of N, P and K. In addition to providing nutrients, tithonia incorporated at 5 t dry matter ha−1 can reduce P sorption and increase soil microbial biomass. Because of high labor requirements for cutting and carrying the biomass to fields, the use of tithonia biomass as a nutrient source is more profitable with high-value crops such as vegetables than with relatively low-valued maize. The transfer of tithonia biomass to fields constitutes the redistribution of nutrients within the landscape rather than a net input of nutrients. External inputs of nutrients would eventually be required to sustain production of tithonia when biomass is continually cut and transferred to agricultural land.
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    URL: http://dx.doi.org/10.1023/A:1006339025728
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    Two isoforms of soluble auxin receptor in rice (Oryza sativa L.) plants: Binding property for auxin and interaction with plasma membrane H+-ATPase (2000)
    Springer
    Plant growth regulation 32 (2000), S. 143-150 
    Details
    ISSN: 1573-5087
    Keywords: auxin binding isotherm ; auxin receptor ; dose response curve ; H+-ATPase ; iIndole-3-acetic acid ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An auxin receptor protein, isolated from the soluble fractionsof rice shoots and roots, was characterised in terms of the affinity andspecificity for IAA and the modulating effect onH+-ATPase of plant plasma membrane. The receptor proteingives a biphasic binding isotherm for IAA, indicating the existence ofthe primary and secondary binding sites. The predominant isoform of thereceptor in roots shows much higher affinity to IAA compared with thatin shoots. Being monomeric protein with about the same molecular mass(57–58 kDa) and showing a similar chromatographic behaviour, bothisoforms mediate IAA-induced modulation of the plasma membraneH+-ATPase in the respective IAA concentration rangesseparated by ca. 3 orders of magnitudes(10-10–10-7 M vs.10-7–10-4 M). Analysis of kinetic data ofthe H+-ATPase activity revealed that the receptor perse functions as an effector of the enzyme, causing a decrease inKm and an increase in Vmax through protein-proteininteraction at a 1:1 ratio. Further, it appeared that, while IAAdoes not affect by itself the kinetic parameters of theH+-ATPase, the auxin exerts its effect via thereceptor, biphasically regulating the efficiency of the effectormolecule probably by inducing two-phase conformational changes thatinvolve IAA binding to two separate binding sites. It was also foundthat other active auxins examined, such as indole-3-propionic acid,1-naphthalene acetic acid and 2,4-dichlorophenoxyacetic acid, do notwork together with the receptor to elicit the same response of theH+-ATPase as seen with IAA.
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    URL: http://dx.doi.org/10.1023/A:1010745310101
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    Salinity increases CO2 assimilation but reduces growth in field-grown, irrigated rice (2000)
    Springer
    Plant and soil 218 (2000), S. 1-10 
    Details
    ISSN: 1573-5036
    Keywords: dry matter reduction ; leaf chlorophyll content ; leaf sodium uptake ; Oryza sativa ; photosynthesis ; salinity ; season effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Salinity is a major yield-reducing factor in coastal and arid, irrigated rice production systems. Salt tolerance is a major breeding objective. Three rice cultivars with different levels of salt tolerance were studied in the field for growth, sodium uptake, leaf chlorophyll content, specific leaf area (SLA), sodium concentration and leaf CO2 exchange rates (CER) at photosynthetic active radiation (PAR)-saturation. Plants were grown in Ndiaye, Senegal, at a research station of the West Africa Rice Development Association (WARDA), during the hot dry season (HDS) and the wet season (WS) 1994 under irrigation with fresh or saline water (flood water electrical conductivity = 3.5 mS cm-1). Relative leaf chlorophyll content (SPAD method) and root, stem, leaf blade and panicle dry weight were measured at weekly intervals throughout both seasons. Specific leaf area was measured on eight dates, and CER and leaf sodium content were measured at mid-season on the first (topmost) and second leaf. Salinity reduced yields to nearly zero and dry-matter accumulation by 90% for the susceptible cultivar in the HDS, but increased leaf chlorophyll content and CER at PAR- saturation. The increase in CER, which was also observed in the other cultivars and seasons, was explained by a combination of two hypotheses: leaf chlorophyll content was limited by the available N resources in controls, but not in salt-stressed plants; and the sodium concentrations were not high enough to cause early leaf senescence and chlorophyll degradation. The growth reductions were attributed to loss of assimilates (mechanisms unknown) that must have occurred after export from the sites of assimilation. The apparent, recurrent losses of assimilates, which were between 8% and 49% according to simulation with the crop model for potential yields in irrigated rice, ORYZA S, might be partly due to root decomposition and exudation. Possibly more importantly, energy-consuming processes, such as osmoregulation, interception of sodium and potassium from the transpiration stream in leaf sheaths and their subsequent storage, drained the assimilate supply.
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    URL: http://dx.doi.org/10.1023/A:1014953504021
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    Carbon dioxide enrichment and nitrogen fertilization effects on cotton (Gossypium hirsutum L.) plant residue chemistry and decomposition (2000)
    Springer
    Plant and soil 220 (2000), S. 89-98 
    Details
    ISSN: 1573-5036
    Keywords: Carbohydrates ; CO2 ; lignin ; nitrogen ; proanthocyanidins ; soil respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Increased atmospheric carbon dioxide (CO2) concentration will likely cause changes in plant productivity and composition that might affect soil decomposition processes. The objective of this study was to test to what extent elevated CO2 and N fertility-induced changes in residue quality controlled decomposition rates. Cotton (Gossypium hirsutum L.) was grown in 8-l pots and exposed to two concentrations of CO2 (390 or 722 μmol mol-1) and two levels of N fertilization (1.0 or 0.25 g l-1 soil) within greenhouse chambers for 8 wks. Plants were then chemically defoliated and air-dried. Leaf, stem and root residues were assayed for total non-structural carbohydrates (TNC), lignin (LTGA), proanthocyanidins (PA), C and N. Respiration rates of an unsterilized sandy soil (Lakeland Sand) mixed with residues from the various treatments were determined using a soda lime trap to measure CO2 release. At harvest, TNC and PA concentrations were 17 to 45% higher in residues previously treated with elevated CO2 compared with controls. Leaf and stem residue LTGA concentrations were not significantly affected by either the elevated CO2 or N fertilization treatments, although root residue LTGA concentration was 30% greater in plants treated with elevated CO2. The concentration of TNC in leaf residues from the low N fertilization treatment was 2.3 times greater than that in the high N fertilization treatment, although TNC concentration in root and stem residues was suppressed 13 to 23% by the low soil N treatment. PA and LTGA concentrations in leaf, root and stem residues were affected by less than 10% by the low N fertilization treatment. N concentration was 14 to 44% lower in residues obtained from the elevated CO2 and low N fertilization treatments. In the soil microbial respiration assay, cumulative CO2 release was 10 to 14% lower in soils amended with residues from the elevated CO2 and low N fertility treatments, although treatment differences diminished as the experiment progressed. Treatment effects on residue N concentration and C:N ratios appeared to be the most important factors affecting soil microbial respiration. The results of our study strongly suggest that, although elevated CO2 and N fertility may have significant impact on post-harvest plant residue quality of cotton, neither factor is likely to substantially affect decomposition. Thus, C cycling might not be affected in this way, but via simple increases in plant biomass production.
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    Nutrient limitations in wet, drained and rewetted fen meadows: evaluation of methods and results (2000)
    Springer
    Plant and soil 220 (2000), S. 35-47 
    Details
    ISSN: 1573-5036
    Keywords: drainage ; fertilisation ; nature management ; nitrogen ; nutrient contents ; nutrients ; peat ; phosphate ; potassium ; restoration ; rewetting ; shoot biomass ; species richness ; wetlands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Restoration of wet grassland communities on peat soils involves management of nutrient supply and hydrology. The concept of nutrient limitation was discussed as well as its interaction with drainage and rewetting of severely drained peat soils. Different methods of assessing nutrient limitation were compared and the type and extent of nutrient limitation were determined for several wet grassland communities. It was concluded that a full-factorial field fertilisation experiment is the most preferable method. Plant tissue analyses and soil chemical analyses were considered less suitable, although they may provide helpful additional information. Fertilisation experiments in the laboratory using sods or using test plants appear to be the proper means to study mechanisms or processes, but have a restricted predictive value for field situations. Generalising the results, it seems that many relativily undisturbed grassland plant communities on peaty soils are characterised by N limitation. Phosphate limitation for vegetation on peat soils is mainly observed in specific circumstances such as extreme calcium richness, high concentrations of Fe or as a result of drainage or long-term hay cropping. The latter two may also cause K limitation. Rewetting is regarded as a prerequisite in restoring wet grassland communities. Further restoration measures to influence nutrient availability depend on aims of the management and the individual site conditions.
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    URL: http://dx.doi.org/10.1023/A:1004735618905
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    Temporal changes in C, P and N concentrations in soil solution following application of synthetic sheep urine to a soil under grass (2000)
    Springer
    Plant and soil 222 (2000), S. 1-13 
    Details
    ISSN: 1573-5036
    Keywords: carbon ; defoliation ; nitrogen ; phosphorus ; sheep urine ; soil solution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We have determined the temporal changes in the concentration of dissolved organic carbon (DOC) and P and N components in soil solution following application of synthetic sheep urine (500 kg N ha-1) to a brown forest soil in boxes sown with Agrostis capillaris. Three contrasting defoliation treatments (no cutting, single cut before urine application and regular cutting twice per week) plus a fallow soil were studied. The synthetic urine contained 15N labelled urea and was P-free. Intact soil cores were taken after 2, 7, 14, 21 and 56 d and centrifuged to obtain soil solution. The urea in the synthetic urine was rapidly hydrolysed in the soil, increasing soil solution pH, DOC and total dissolved phosphorus (TDP) concentrations. For the regularly defoliated sward, DOC and P reached maximum concentrations (4000 mg DOC L-1 and 59 mg TDP L-1) on day 7. From their peak values, pH and DOC and P concentrations generally decreased with time and at day 56 were near those of the control. Concentrations of NH4 + and NO3 - in the no-urine treatments fluctuated and the greatest treatment differences were between the fallow soil and the soil sown with grass. Adding synthetic urine increased NH4 + concentrations during the first week, but NO3 - concentrations decreased. This was consistent with the 15N labelling of the NO3 - pool which required 3 weeks to reach that of 15NH4 +. Dissolved organic nitrogen (DON) reached a maximum value at day 7 with a concentration of 409 mg N L-1. The DON in soil solution contained no detectable amounts of 15N label indicating that it was derived from sources in the soil. Differences in soil solution composition related to the effect of the other cutting treatments and the fallow treatment were small compared to the effect of synthetic urine addition.
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    URL: http://dx.doi.org/10.1023/A:1004799323646
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    Spatial heterogeneity of soil respiration and related properties at the plant scale (2000)
    Springer
    Plant and soil 222 (2000), S. 203-214 
    Details
    ISSN: 1573-5036
    Keywords: carbon ; geostatistics ; nitrification potential ; nitrogen ; pH ; root biomass ; soil respiration ; spatial Heterogeneity ; variability scale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Geostatistical techniques were used to quantify the scale and degree of soil heterogeneity in 2 m2 plots around 9-year-old poplar trees and within a wheat field. Samples were taken during two years, on an unaligned grid, for analysis of soil respiration, C and N content, available P, gravimetric moisture, pH, nitrification potential, and root biomass. Kriged maps of soil respiration, moisture, and C content showed strong spatial structure associated with poplar trees but not with wheat rows. All soil properties showed higher autocorrelation in June than in April. Isopleth patchiness for all variates was less in June. This was associated with lower respiration rates due to lower litter decomposition. From the degree and scale of heterogeneity seen in this study, we conclude that the main causes of soil heterogeneity at this scale (2 m2) are likely to be found at micro scales controlled in part by plant root and plant residue patterns. These must be understood in the evaluation of ecosystem processes.
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    URL: http://dx.doi.org/10.1023/A:1004757405147
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    Long-term effects of elevated atmospheric CO2 on below-ground biomass and transformations to soil organic matter in grassland (2000)
    Springer
    Plant and soil 224 (2000), S. 85-97 
    Details
    ISSN: 1573-5036
    Keywords: carbon ; CO2 enrichment ; nitrogen ; particulate organic matter ; roots ; tallgrass prairie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We determined the effects of elevated [CO2] on the quantity and quality of below-ground biomass and several soil organic matter pools at the conclusion of an eight-year CO2 enrichment experiment on native tallgrass prairie. Plots in open-top chambers were exposed continuously to ambient and twice-ambient [CO2] from early April through late October of each year. Soil was sampled to a depth of 30 cm beneath and next to the crowns of C4 grasses in these plots and in unchambered plots. Elevated [CO2] increased the standing crops of rhizomes (87%), coarse roots (46%), and fibrous roots (40%) but had no effect on root litter (mostly fine root fragments and sloughed cortex material 〉500 μm). Soil C and N stocks also increased under elevated [CO2], with accumulations in the silt/clay fraction over twice that of particulate organic matter (POM; 〉53 μm). The mostly root-like, light POM (density ≤1.8 Mg m-3) appeared to turn over more rapidly, while the more amorphous and rendered heavy POM (density 〉1.8 Mg m-3) accumulated under elevated [CO2]. Overall, rhizome and root C:N ratios were not greatly affected by CO2 enrichment. However, elevated [CO2] increased the C:N ratios of root litter and POM in the surface 5 cm and induced a small but significant increase in the C:N ratio of the silt/clay fraction to a depth of 15 cm. Our data suggest that 8 years of CO2 enrichment may have affected elements of the N cycle (including mineralization, immobilization, and asymbiotic fixation) but that any changes in N dynamics were insufficient to prevent significant plant growth responses.
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    URL: http://dx.doi.org/10.1023/A:1004771805022
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    Influence of decomposer food web structure and nitrogen availability on plant growth (2000)
    Springer
    Plant and soil 225 (2000), S. 153-165 
    Details
    ISSN: 1573-5036
    Keywords: carbon ; decomposer food web ; indirect effects ; microbes ; nitrogen ; plant growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We studied the sensitivity of soil microbial communities and ecosystem processes to variation in the vertical and horizontal structure of decomposer food web under nitrogen poor and N-enriched conditions. Microcosms with humus and litter layer of boreal forest floor, birch seedlings infected with mycorrhizal fungi, and decomposer food webs with differing trophic group and species composition of soil fauna were constructed. During the second growing period for the birch, we irrigated half of the microcosms with urea solution, and the other half with de-ionised water to create two levels of N concentration in the substrate. During the experiment night time respirations of the microcosms were measured, and the water leached through the microcosms was analysed for concentration of mineral N, and nematode numbers. The microcosms were destructively sampled after 37 weeks for plant biomass and N uptake, structure of soil animal and microbial community (indicated by PLFA profiles), and physical and chemical properties of the humus and litter materials. Predatory mites and nematodes had a negative influence on the biomass of their microbivorous and microbi-detritivorous prey, and microbi-detritivores affected the biomass and community structure of microbes (indicated by PLFA-analysis). Moreover, predatory mites and nematodes increased microbial biomass and changed the microbial community structure. The decomposer food web structure affected also N uptake and growth of plants. Microbi-detritivorous fauna had a positive effect, whereas predators of microbial and detritus feeding fauna exerted a negative influence on plant N uptake and biomass production. The impact of a trophic group on the microbes and plant was also strongly dependent on species composition within the group. Nitrogen addition magnified the influence of food web structure on microbial biomass and plant N uptake. We suggest that addition of urea-N to the soil modified the animal-microbe interaction by increasing microbial growth and altering community structure of microbes. The presence of microbi-detritivores and predators reduced loss of carbon from the microcosms, and the food web structure influenced also water holding capacity of the materials. The changes in plant growth, nutrient cycling, size of N and C pools, and in the physical properties of the soil emphasize the importance and diversity of indirect consequences of decomposer food web structure.
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    Greenhouse and field trials of the bacterial antagonists in pelletformulations to suppress sheath blight of rice caused by Rhizoctoniasolani (2000)
    Springer
    BioControl 45 (2000), S. 245-256 
    Details
    ISSN: 1573-8248
    Keywords: biological control ; Oryza sativa ; rice sheath blight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bacterial formulations, produced using both Bacillus megaterium andB. pumilus individually with pharmaceutical technology, were testedunder both greenhouse and field conditions. In the greenhouse testing,some bacterial formulations, for instance For 7 minus Lac and For 16 minusLac, performed as well as freshly prepared bacterial antagonists insuppress sheath blight disease. In the field testing, For 16 minus Lac wasnot effective in suppressing sheath blight development. Failure of the For16 minus Lac to suppress sheath blight disease in the field trial may be dueto the dilution and inactivation of antibiotics produced by B.megaterium in the aquatic environment in the rice field and climaticconditions during the formulation application.
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    URL: http://dx.doi.org/10.1023/A:1009948404423
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    Effect of split applications of cattle slurry and mineral fertilizer–N on the yield of silage maize in a slurry-based cropping system (1999)
    Springer
    Nutrient cycling in agroecosystems 53 (1999), S. 209-218 
    Details
    ISSN: 1573-0867
    Keywords: cattle slurry ; fertilizer splitting ; nitrogen ; recovery ; residual nitrogen ; Zea mays L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The recovery of soil mineral nitrogen (N) by crops, and its subsequent utilisation for dry matter (DM) production may be increased when the application of N is postponed until after crop emergence. The significance of this strategy for silage maize was studied in nine field experiments on Dutch sandy soils from 1983 to 1988. In five experiments the effect of slurry applied before planting at a rate of circa 66 m3 ha-1, was compared to the effect of a similar rate of which half was applied before planting and half at the 4–6 leaf stage. In the 4-6 leaf stage slurry was either injected or banded. In four other experiments the effect of mineral fertilizer-N splitting was studied. In these experiments, 30 m3 ha-1 cattle slurry, applied before planting, was supplemented with mineral fertilizer-N at rates ranging from 40 to 160 kg ha-1, either fully applied before crop emergence or split. When split, 40 kg ha-1 of the mineral fertilizer-N rate was banded at the 4–6 leaf stage. According to balance sheet calculations, substantial losses of slurry N and mineral fertilizer-N occurred during the growing season. Losses were compensated for, however, by apparent mineralization, ranging from 0.34 to 0.77 kg N ha-1 day-1. Split applications of cattle slurry had a significant positive effect on the DM yield in two out of five experiments compared to the conventional non-split application, but only when the post-emergence slurry application was banded which is no longer in accordance with present legislation. Split applications of mineral fertilizer-N had a significant positive effect in one experiment where rainfall was excessive but not in the others. The results provide insufficient evidence to recommend farmers to split applications. Soil mineral N sampling at the 4–6 leaf stage should hence be considered a control on the appropriateness of early N applications after exceptional weather conditions rather than a routine observation on which the post-emergence N dressing is to be based in a deliberate splitting strategy. Our data suggest that the financial return of a 40 kg ha-1 supplementation with mineral fertilizer-N, was questionable when more than 175 kg N ha-1 were found in the upper 0.6 m soil layer at the 4–6 leaf stage.
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    Using nitrogen budgets to indicate nitrogen use efficiency and losses from whole farm systems: a comparison of three methodological approaches (1999)
    Springer
    Nutrient cycling in agroecosystems 53 (1999), S. 259-267 
    Details
    ISSN: 1573-0867
    Keywords: farming systems ; nitrogen ; nitrogen budgets ; sustainability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three approaches to nitrogen budgeting were developed and their ability to quantitatively describe nitrogen cycling in a fertilizer based and a grass–clover based beef system tested. Budgets ranged in complexity from the Economic Input:Output (EIO) budget, which accounted simply for purchases and sales of nitrogen over the farmgate, through the Biological Input:Output (BIO) budget, which included estimates of biological nitrogen fixation and attempted to partition losses into leaching and gaseous forms, to the Transfer:Recycle:Input:Output (TRIO) budget, which also accounted for key soil processes. Nitrogen unaccounted for in the fertilized system decreased with increasing budget complexity (285, 212 and 188 kg ha-1 yr-1 unaccounted for by the EIO, BIO and TRIO budgets, respectively). In the legume based grass–clover system, the EIO budget did not accurately describe total nitrogen inputs as it did not include 146 kg ha-1 yr-1 from symbiotic nitrogen fixation. In the grass–clover system, nitrogen unaccounted for was again greater using the BIO than the TRIO budget (103 and 79 kg ha-1 yr-1, respectively). In conclusion, the most complex budgeting approach (TRIO) was able to account for the fate of a greater proportion of nitrogen inputs than the simpler approaches. However, the perceived success of the different approaches was strongly dependent on the precise objective.
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    Nitrogen uptake and chlorophyll meter measurements in Spring Wheat (1999)
    Springer
    Nutrient cycling in agroecosystems 55 (1999), S. 1-6 
    Details
    ISSN: 1573-0867
    Keywords: chlorophyll meter ; irrigation ; LAI ; nitrogen ; Spring Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A Spring Wheat crop was grown using three irrigation levels and nitrogen rate applications to evaluate chlorophyll meter measurements as a possible nitrogen nutrition index for modelling. These measurements yielded the most reliable indications at Zadoks GS45. The lower limit indicating severe nitrogen deficiency in the leaves was approximately 35 SPAD units while the upper limit of 45 SPAD units, indicated an excess consumption.
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    The fate of algal nitrogen in a flooded soil system (1999)
    Springer
    Nutrient cycling in agroecosystems 55 (1999), S. 89-94 
    Details
    ISSN: 1573-0867
    Keywords: algae ; flooded soils ; N cycling ; nitrogen ; 15N ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Algal N labelled with 15N added to a flooded soil in laboratory columns without plants was studied to determine the changes over time in the fate of N assimilated by algae and to study how its fate is affected by (a) exclusion of light simulating complete closure of the rice canopy, and (b) addition of fertilizer-NH4 *. In the light but with no added fertilizer-N there was little net mineralization of the added algal N during the first 4 weeks, but after 8 weeks 42% had been mineralized, of which 95% was denitrified. Exclusion of light caused net mineralization to proceed more rapidly in the first 4 weeks due to the death of algal cells and lowered reassimilation. After 8 weeks 51% had been mineralized, of which 54% was denitrified, 16% volatilized and 30% was present as KCl exchangeable NH4 +-N. Application of fertilizer-NH4 + apparently caused mineralization of 25% of the algal N within one week but the results were probably affected by pool substitution in which labelled N mineralized to NH4 +-N was diluted with fertilizer – NH+ 4 and then immobilized leaving more labelled NH4–N in the mineral pool. After 8 weeks, 42% of algal N had been mineralized, of which 69% was estimated to have been denitrified, 19% lost through NH3 volatilization and 12% remained as extracted NH4 ++NO- 3. Uptake of N by a rice crop would reduce the gaseous losses. Algal N was mineralized quickly enough to be available during the growing season of a rice crop and, depending on field conditions, algae may have a role in assimilating N and protecting it from loss as well as being a major driving force for NH3 volatilization through diurnal increases in pH.
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    URL: http://dx.doi.org/10.1023/A:1009850915579
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    Improving the field performance of micro-and minitubers (1999)
    Springer
    Potato research 42 (1999), S. 559-568 
    Details
    ISSN: 1871-4528
    Keywords: plastic mulch ; transplanting ; nitrogen ; planting depth ; seed tuber weight ; physiological age ; radiation conversion efficiency ; harvest index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In many seed potato producing areas, micro- and minitubers are too small for direct planting as seed tubers in the field. Such use of these propagules can, however, be feasible if the crop's growth and development can be advanced. Increasing light interception, harvest index and yield of useable progeny tubers has been proved possible with plastic mulch and pre-planting of small tubers in a greenhouse. High amounts of nitrogen (up to 180 kg ha−1) or deep planting (up to 9 cm) were less effective. Using older or pre-sprouted micro- or minitubers may be beneficial, because this might increase the number of sprouts per mother tuber (and thus stems per plant) or advance the growth of sprouts or stems. However, this would require even more careful management, due to the weakness of these sprouts and stems. Micro- and minitubers should be as large as feasible when used for direct planting in the field.
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    URL: http://dx.doi.org/10.1007/BF02358172
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    Effects of Potato-cyst Nematodes (Globodera pallida) and Soil pH on Root Growth, Nutrient Uptake and Crop Growth of Potato (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 61-76 
    Details
    ISSN: 1573-8469
    Keywords: core sampling ; foliar nutrient concentrations ; minirhizotrons ; nitrogen ; phosphorus ; potassium ; Rhizolab
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Potato-cyst nematodes (Globodera pallida) cause severe yield losses in potato. Plants infected with potato-cyst nematodes generally have reduced concentrations of nitrogen, phosphorus and potassium in the foliage. This study investigated whether reduced growth of nematode-infected potato is caused by nutrient limitation. Experiments in the field and in containers showed that phosphorus concentration correlated best with total crop biomass at early stages of growth. The role of phosphorus in nematode damage was further investigated in the field and in the Wageningen Rhizolab. The experimental field was infested with potato-cyst nematodes and two levels of nematode density were established by fumigation with a nematicide. Prior applications of calcium carbonate resulted in pHKCl levels of 4.8 and 6.1. Two levels of phosphorus fertiliser were applied: either 0 or 225 kg P ha−1. In the Wageningen Rhizolab, soil of both pH levels from the field was used after treatment with 1 MRad gamma irradiation to kill the nematodes. Subsequently, half of the soil was inoculated with cysts to give a nematode density of 30 viable juveniles per gram of soil. In the field, nine weeks after planting, the total crop biomass ranged from 107 g m−2 for the treatment with nematodes at pHKCl 6.1 without phosphorus fertiliser to 289 g m−2 for the fumigated treatment at pHKCl 4.8 with phosphorus fertiliser. The differences in total biomass for the various treatments were explained by differences in foliar phosphorus concentration. Nematodes induced or aggravated P deficiency and reduced total biomass. This was not the only damage mechanism as at high, non-limiting levels of foliar phosphorus concentration, nematodes still reduced total biomass. In the Wageningen Rhizolab, directly after planting, the number of roots visible against minirhizotrons was reduced by nematodes. However, the increase of root number in the nematode treatment continued longer than in the control, until root number was higher than that of the control. The compensary root growth of the nematode treatment was restricted to the top 30 cm and nematodes reduced rooting depth. High soil pH reduced growth, mainly by reducing the availability of phosphate. Both nematodes and high soil pH reduced nutrient uptake per unit root length. Our results lead us to suggest an interaction between nematodes and soil pH, with nematode damage being higher at pHKCl 6.1 than at pHKCl 4.8.
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    URL: http://dx.doi.org/10.1023/A:1008641511688
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    Fine Structure of the Early Interaction of Lily Roots with Fusarium oxysporum f.sp. lilii (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 431-443 
    Details
    ISSN: 1573-8469
    Keywords: basal rot ; cell wall degradation ; Lilium ; pathogenesis ; plasmolysis ; resistance ; transfer cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The early interaction of lily roots with the cortical rot pathogen Fusarium oxysporum f.sp. lilii was studied using roots of lily bulblets grown in Hoagland's solution, inoculated with the pathogen, and sampled up to 48 h later. Conidia produced germ tubes within 6 h, which extended towards and into the mucilage covering the root elongation zone, and along and into the anticlinal grooves and middle lamellae of epidermal cells. By 24–48 h, infecting hyphae had reached the periclinal walls and intercellular spaces between the epidermis and the outermost cells of the cortex. Penetration of intercellularly growing hyphae directly across host cell walls was not observed; invasion of the cell lumen only occurred by gradual infringing of hyphae upon successive primary wall layers. Non-cellulosic wall appositions rich in vesicles and covered by a cellulosic protective-like layer were formed in response to approaching hyphae in resistant cv.Connecticut King, but rarely in susceptible cv. Esther which seemed more susceptible to plasmolysis and rot. Finger-like projections of the appositions into the host cell cytoplasm likely represent early stages of transfer cell formation.
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    URL: http://dx.doi.org/10.1023/A:1008749514517
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    Different Patterns of Host Genes are Induced in Rice by Pseudomonas syringae, a Biological Inducer of Resistance, and the Chemical Inducer Benzothiadiazole (BTH) (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 659-665 
    Details
    ISSN: 1573-8469
    Keywords: Magnaporthe grisea ; Pseudomonas syringae pv. syringae ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Rice seedlings treated with the synthetic compound benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH) acquired resistance to subsequent attack by the rice blast fungus Magnaporthe grisea (Hebert) Barr. BTH (trade name Bion™) has been released to the market as a plant protecting agent for rice. Here, we analysed the pattern of expressed genes in rice plants treated with BTH, and compared this pattern with those induced by the formerly discovered resistance inducer 2,6-dichloroisonicotinic acid (INA) and by Pseudomonas syringae pv. syringae, a non-host pathogen inducing a hypersensitive response. Both INA and BTH induced similar patterns of genes, suggesting that these compounds are functional analogues. In contrast, the patterns induced by the chemical inducers and by P. syringae were clearly dissimilar.
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    URL: http://dx.doi.org/10.1023/A:1008791223608
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    Effects of Temperature on Infection in Capsicum sp. and Nicotiana benthamiana by Impatiens Necrotic Spot Tospovirus (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 509-512 
    Details
    ISSN: 1573-8469
    Keywords: tospovirus ; resistance ; Capsicum sp. ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Infection by two isolates of impatiens necrotic spot tospovirus (INSV) under temperature regimes of 25/18°C (day/night) or 33°C (continuous) was studied in Capsicum annuum (systemically susceptible to tomato spotted wilt tospovirus, TSWV), C. chinense PI 152225 and PI 159236 (reacting hypersensitively to TSWV) and Nicotiana benthamiana (systemically susceptible to both tospoviruses). At 25/18°C infection was systemic in all hosts tested. At 33°C infection in N. benthamiana was systemic whereas in C. annuum and C. chinense it was restricted to the inoculated leaves. The result differed from that reported for TSWV, where high temperature made plants more susceptible, or caused no difference. Exchanging temperature regimes 6 h to 4 days after inoculation did not affect the final results one month later, with plants being only locally infected at 33°C continuous regime, or systemically infected at 25/18°C alternate regime. The two INSV isolates were biologically and serologically stable for 5 passages in N. benthamiana held continuously at 33°C.
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    URL: http://dx.doi.org/10.1023/A:1008742516820
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    A Laboratory Assay for Phytophthora infestans Resistance in Various Solanum Species Reflects the Field Situation (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 241-250 
    Details
    ISSN: 1573-8469
    Keywords: Phytophthora infestans ; potato ; wild Solanum spp. ; resistance ; detached leaves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Physiological and molecular research on resistance responses of Solanum tuberosum cultivars and partially resistant Solanum species to Phytophthora infestans requires a reliable resistance test that can be used in the laboratory. Laboratory tests performed on detached leaves and intact plants were compared with field tests for similarity of late blight reactions. Detached leaves from field-grown plants were as resistant as detached leaves from climate chamber-grown plants when challenged with P. infestans. However, detached leaves incubated in covered trays at high relative humidity were more susceptible than detached leaves kept in open trays or leaves on intact plants. The incubation conditions of detached leaves in covered trays rather than detachment itself appeared to affect the resistance expression. Detached leaves of some wild Solanum genotypes became partially infected, whereas intact plants were completely resistant when inoculated. Inoculation of leaves on intact plants, however, resulted in lower infection efficiencies. These limitations should be taken into account when choosing the appropriate inoculation method for specific purposes. For resistance screening, laboratory tests proved to be a good alternative for field tests. The ranking of resistance levels for twenty plant genotypes was similar under laboratory and field conditions.
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    URL: http://dx.doi.org/10.1023/A:1008710700363
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    The effect of regeneration burns on the growth, nutrient acquisition and mycorrhizae of Eucalyptus regnans F. Muell. (mountain ash) seedlings (1999)
    Springer
    Plant and soil 210 (1999), S. 273-283 
    Details
    ISSN: 1573-5036
    Keywords: ectomycorrhizae ; Eucalyptus regnans ; forest burns ; nitrogen ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was conducted to compare the effects on the growth of Eucalyptus regnans seedlings of unheated soil and soil heated to different extents (as indicated by soil colour–bright red or black) in burnt logging coupes, and to separate the effects of heating of the soil on direct nutrient availability and on morphotypes and effectiveness of ectomycorrhizae. Burnt soils were collected from three logging coupes burnt 2, 14 and 25 months previously and unbumt soil from adjacent regrowth forests. Compared to unburnt soil, the early seedling growth was stimulated in black burnt soil from all coupes (burnt 2, 14 and 25 months previously). Seedling growth was generally poor in red burnt soil, especially in soil collected 2 months after burning. However, the concentration of extractable P was extremely high in red burnt soil, especially in soil collected 2 months after burning. In black burnt soil, extractable P was increased in soil 2 months after burning, but not in the soils collected 14 or 25 months after burning. However, both total P content and concentration in seedlings were increased in all collections of black burnt soil. Frequency of ectomycorrhizae was high in seedlings grown in all black burnt soils, but the mycorrhizal mantles were poorly developed in seedlings in black burnt soil collected 2 months after burning. Seedlings were also ectomycorrhizal in red burnt soil, except in soil collected 2 months after burning. Fine root inocula from seedlings grown in black burnt soils collected 14 and 25 months after burning significantly stimulated both seedling growth and P uptake compared with the uninoculated control, whereas the fine root inocula from the seedlings grown in all the other soils did not. These results suggest that, in black burnt soil, both direct nutritional changes and changes in the ectomycorrhizae may contribute to seedling growth promotion after regeneration burns. The generally poor seedling growth in red burnt soils is likely to have been due to N deficiency as the seedlings in these soils were yellow-green and the tissue concentrations of N were significantly lower than in other treatments.
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    URL: http://dx.doi.org/10.1023/A:1004609912315
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    The use of microsatellite polymorphisms for the identification of Australian breeding lines of rice (Oryza sativa L.) (1999)
    Springer
    Euphytica 108 (1999), S. 53-63 
    Details
    ISSN: 1573-5060
    Keywords: microsatellite ; Oryza sativa ; polymorphism ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ten microsatellite loci were analysed for 43 cultivars or breeding lines of rice. Polymorphism-Information-Content values ranged from 0.62 to 0.92. The microsatellite markers were found to be useful for cultivar identification and assessment of genetic relationships. Most of the cultivars could be uniquely identified by at least one microsatellite marker. Genetic heterogeneity was detected within rice samples by amplification of microsatellites from DNA extracted from multiple individual plants and also from bulked DNA preparations.
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    URL: http://dx.doi.org/10.1023/A:1003688612179
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    Search for molecular markers linked to Liriomyza trifolii resistance in tomato (1999)
    Springer
    Euphytica 109 (1999), S. 149-156 
    Details
    ISSN: 1573-5060
    Keywords: BSA ; Liriomyza trifolii ; Lycopersicon ; molecular markers ; resistance ; leaf miner
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Resistance to many arthropods, including Liriomyza species, is known to be present in accessions of Lycopersicon hirsutum (f. typicum or f. glabratum). From the cross L. esculentum cv. Moneymaker and L. hirsutum f. glabratum G1561 100 F2 plants were screened in a no-choice test for resistance to Liriomyza trifolii. The Bulked Segregant Analysis approach was used to find Random Amplified Polymorphic DNA markers linked to resistance. Two markers were located on chromosome 2. Restriction Fragment Length Polymorphisms constructed a more detailed genetic linkage map for part of chromosome 2. Kruskal-Wallis analysis showed that this chromosome harbored a Quantitative Trait Locus (QTL) for number of pupae, number of mines and damage. At least one major QTL is essential for resistance and this QTL is located on chromosome 2 nearby the location of the tomato probe TG451.
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    Agro-morphological characterization of ovary culture-derived plants of rice (Oryza sativa L.) (1999)
    Springer
    Euphytica 106 (1999), S. 197-203 
    Details
    ISSN: 1573-5060
    Keywords: doubled haploids ; genetics ; haploids ; induced variation ; Oryza sativa ; ovary culture ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plants derived from unpollinated ovary culture of ten rice genotypes showed significant variability in agro-morphological characteristics. The ovary-derived plant (H1) populations were completely haploid, doubled haploid or haploid-doubled haploid mixture. Haploids had very drastic reduction in plant height, panicle length, grain length, breadth and number and spikelet fertility (0.0%–2.1%). Doubled haploids from the hybrid of UPRI 95–121 × UPRI 95–165 were normal with fertility ranging between 69.6% and 97.7%. A genetic segregation in ratio of 1:1 was observed for five pigmentation characters in the H1 population derived from hybrid UPRI 95–122 × UPRI 95–165. Plant height showed the largest coefficient of variability (28.5%) followed by the number of spikelets per panicle (24.2%), number of grains per panicle (22.0%), percent seed set (9.2%) and panicle length (9.0%). The range of variation in the H1 population from fully fertile hybrid PMS 2A (CMS) × IR 31802 (restorer) was similar to its corresponding F2 population for plant height, spikelet fertility and number of grains/panicle. A single clone of plants from the cultivar BG 1321 exhibited complete male sterility but normal female fertility when pollinated with other varieties. Ovary-derived plants from the CMS lines PMS 2A and IR 58025A showed stable male sterility and those from thermosensitive genetic male sterile line UPRI 95–140 showed thermosensitive genetic male sterility. These lines have potential in the hybrid breeding program and are being currently exploited.
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    URL: http://dx.doi.org/10.1023/A:1003524217816
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    A comparison of direct and indirect 15N isotope techniques for estimating crop N uptake from organic residues (1999)
    Springer
    Plant and soil 208 (1999), S. 259-270 
    Details
    ISSN: 1573-5036
    Keywords: crop residues ; isotope dilution ; 15N ; nitrogen ; organic matter ; pool substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Experiments were carried out to compare the direct approach for estimating crop N uptake from 15N labelled organic inputs, to two indirect approaches, 15N isotope dilution and A value. In the first experiment soils received 25, 50, 75, or 100 mg N kg soil−1 in the form of Casuarina equisitifolia residues in addition to ammonium sulphate fertiliser, to give a total of 100 mg N kg soil−1 added. This was a cross labelling design, thus two matching sets of treatments, were set up, identical in all but the position of the 15N label. Maize (Zea mays L.) plants were grown in the soils amended with residues for 11 weeks and N derived from residues (Ndfr) estimated using the A-value or the direct approach. The A-value approach appeared to significantly overestimate %Ndfr compared to the direct method. In the second experiment contrasting residues were added to soil, fababean (Vicia faba L. var. minor), alfalfa (Medicago sativa L.), soyabean fixing, (Glycine max (L.) Merrill), soyabean non-fixing, barley (Hordeum vulgare L.) and maize. This was also cross-labelling design, labelled and unlabelled residues were used. Maize plants were grown in these soils for 11 weeks and %Ndfr in the maize plants estimated using 15 N isotope dilution and the direct approach. The 15 N isotope dilution approach also overestimated %Ndfr compared to the direct method in this experiment. Pool substitution appeared to be responsible for the discrepancy between the direct and indirect techniques. It was concluded that 15N isotope dilution and A-value approaches as used in these experiments (i.e where residues and 15N label are added simultaneously) were not appropriate techniques for estimating N derived from organic residues in soils.
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    URL: http://dx.doi.org/10.1023/A:1004529711231
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    Timing of nitrogen uptake affects winter storage and spring remobilisation of nitrogen in nectarine (Prunus persica var. nectarina) trees (1999)
    Springer
    Plant and soil 211 (1999), S. 149-153 
    Details
    ISSN: 1573-5036
    Keywords: nitrogen ; Prunus ; remobilisation ; storage ; uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two-year old nectarine trees (Prunus persica, Batsch, var. nectarina, cv. Starkredgold on GF305 rootstock) planted in pots each received five applications of 1.0 g 15N labelled urea either from mid May to mid July (early uptake) or from mid August to the beginning of October (late uptake). All trees were supplied with a corresponding amount of unlabelled urea when they did not receive the labelled N. In autumn, all abscised leaves were collected and during winter randomly selected trees were harvested and divided into main organs. The remaining trees were transplanted into similar pots filled with sand; they received no N fertiliser and were harvested in May to evaluate the remobilisation of N. Total N and 15N abundance were determined in each organ. Nectarine trees took up similar amounts of N in the 'early' and in the 'late' period; however, more labelled nitrogen was recovered in the perennial organs during the winter when trees received the labelled N in the 'late' than in the 'early' period. Some 73–80% of the N present in the dormant trees was stored in the roots, which contained almost twice the amount of labelled N taken up 'late' than that absorbed 'early'. Nitrogen for spring growth was remobilised predominantly from the roots and accounted for some 43–49% of the labelled N recovered in the tree during winter. Results suggest that the nitrogen taken up 'late' in the season is preferentially stored in roots and used by peach trees to sustain new growth the following spring.
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    URL: http://dx.doi.org/10.1023/A:1004698422522
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    Effects of sulphur nutrition on growth and nitrogen fixation of pea (Pisum sativum L.) (1999)
    Springer
    Plant and soil 212 (1999), S. 207-217 
    Details
    ISSN: 1573-5036
    Keywords: legume ; nitrogen ; N2-fixation ; pea ; sulphur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A S-deficient soil was used in pot experiments to investigate the effects of S addition on growth and N2-fixation in pea (Pisum sativum L.). Addition of 100 mg S pot−1 increased seed yield by more than 2-fold. Numbers of pods formed were the most sensitive yield component affected by S deficiency. Sulphur addition also increased the concentration of N in leaves and stems, and the total content of N in the shoots. The amounts of N fixed by pea were determined at four growth stages from stem elongation to maturity, using the 15N dilution technique. Sulphur addition doubled the amount of N fixed at all growth stages. In contrast, leaf chlorophyll content and shoot dry weight were increased significantly by S addition only after the flowering and pod fill stage, respectively. Pea roots were found to have high concentrations of S, reaching approximately 10 mg g−1 dry weight and being 2.6–4.4 times the S concentration in the shoots under S-sufficient conditions. These results suggest that roots/nodules of pea have a high demand for S, and that N2-fixation is very sensitive to S deficiency. The effects of S deficiency on pea growth were likely to be caused by the shortage of N, due to decreased N2-fixation.
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    URL: http://dx.doi.org/10.1023/A:1004618303445
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    Persistence of tree related patterns in soil nutrients following slash-and-burn disturbance in the tropics (1999)
    Springer
    Plant and soil 209 (1999), S. 137-157 
    Details
    ISSN: 1573-5036
    Keywords: fire ; nitrogen ; phosphorus ; soil nutrient heterogeneity ; tree effects ; tropical dry forest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Individual trees are known to influence soil chemical properties, creating spatial patterns that vary with distance from the stem. The influence of trees on soil chemical properties is commonly viewed as the agronomic basis for low-input agroforestry and shifting cultivation practices, and as an important source of spatial heterogeneity in forest soils. Few studies, however, have examined the persistence of the effects of trees on soil after the pathways responsible for the effects are removed. Here, we present evidence from a Mexican dry forest indicating that stem-related patterns of soil nutrients do persist following slash-and-burn removal of trees and two years of cropping. Pre-disturbance concentrations of resin extractable phosphorus (P), bicarbonate extractable P, NaOH extractable P, total P, total nitrogen (N) and carbon (C), KCl extractable nitrate (NO3 -), and net N mineralization and nitrification rates were higher in stem than dripline soils under two canopy dominant species of large-stemmed trees with contrasting morphologies and phenologies (Caesalpinia eriostachys Benth. and Forchhammeria pallida Liebm.). These stem effects persisted through slash burning and a first growing season for labile inorganic and organic P, NaOH inorganic P, and plant-available P, and through a second growing season for labile organic P, NaOH organic P, and plant-available P. While stem effects for extractable NO3 -, net nitrification rates, total N and C disappeared after felling and slash burning, these stem effects returned after the first growing season. These results support the view that tree-influenced patterns of soil nutrients do persist after tree death, and that trees contribute to the long-term spatial heterogeneity of forest soils.
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    URL: http://dx.doi.org/10.1023/A:1004503023973
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    Nitrogen and phosphorus requirement of the microbiota in soils of the Bornhöved Lake district (1999)
    Springer
    Plant and soil 212 (1999), S. 173-181 
    Details
    ISSN: 1573-5036
    Keywords: nitrogen ; phosphatase activity ; phosphorus ; protease activity ; soil microbial biomass ; substrate-induced respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Estimating in situ N and P status of the soil microbiota is complicated because microbiological features reflect potentials rather than field conditions. Complementary microbiological assays were, therefore, combined to evaluate the N and P requirement of the microbiota in seven agricultural, grassland and forest topsoils of the Bornhöved Lake district as follows: (i) the sensitivity of the substrate-induced respiration (SIR) to supplemental addition of N and P was monitored during microbial growth and (ii) soil protease and phosphatase activities were analysed and related to soil mass and microbial biomass content. Nitrogen addition increased the maximal SIR rate in all except one soil indicating that the growth of organisms is restricted by this element when easily degradable C source is present. Supplemental N (and in some cases also P) retarded the respiratory response within the first 24 h which suggests microbial sensitivity and/or greater anabolic efficiency. With additional N the maximal SIR rate was most strongly enhanced in topsoils of the beech forest and the dystric alder forest. Thus, the microbial growth in these soils that were below litter horizons seems to be mostly restricted by N. Supplemental P positively affected respiratory response of soils under monoculture, wet grassland and dystric alder forest. In the dystric alder forest soil, high rates of alkaline and unbuffered phosphatase activity were observed when activity was related to either soil mass or microbial biomass content. The data of proteolytic and phospholytic enzymes are discussed with reference to nutrient deficiency and microbial strategy for N and P adsorption.
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    URL: http://dx.doi.org/10.1023/A:1004677427165
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    Effects of aluminium treatment on Norway spruce roots: Aluminium binding forms, element distribution, and release of organic substances (1999)
    Springer
    Plant and soil 216 (1999), S. 103-116 
    Details
    ISSN: 1573-5036
    Keywords: aluminium ; binding forms ; element compartmentation ; Picea abies ; resistance ; root exudates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to investigate if Al resistance in Norway spruce (Picea abies[L.] Karst.) can be attributed to similar exclusion mechanisms as they occur in several crop plants, three-year-old Norway spruce plants were treated for one week in hydroculture with either 500 μM AlCl3 or CaCl2 solutions at pH 4. Sequential root extraction with 1 M NH4Cl and 0.01 M HCl and EDX microanalysis revealed that Al and Ca in cell walls and on the surface participated in exchange processes. About half of the Al extracted by the sequential extraction was not exchangeable by 1 M NH4Cl. Phenolics and phosphate present in the root extracts are possible ligands for Al adsorbed to or precipitated at the root in a non-exchangeable form. In both treatments, C release during the first period of 2 d was much higher than during the remaining time of the experiment. Al treated plants released less total C, carbohydrates and phenolics than did Ca treated plants. Acetate was the only organic acid anion that could be detected in some samples of both treatments. Free amino acids were present at micromolar concentrations but as hydrolysis did not increase their yield, there was no evidence of peptide release. One to two thirds of the released C were large enough not to pass a 1 kDa ultrafilter. The results suggest that exudation of soluble organic complexors is not a major Al tolerance mechanism in Norway spruce, although complexation of Al by phenolic substances released by the root could be detected by fluorescence spectroscopy. Aluminium tolerance could rather be attributed to immobilization in the root apoplast, where strong binding sites are available or precipitation may occur.
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    URL: http://dx.doi.org/10.1023/A:1004728122261
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    Contributions to nitrogen leaching and pasture uptake by autumn-applied dairy effluent and ammonium fertilizer labeled with 15N isotope (1999)
    Springer
    Plant and soil 210 (1999), S. 189-198 
    Details
    ISSN: 1573-5036
    Keywords: effluent ; leaching ; 15N isotope ; nitrogen ; pasture ; uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The objective of this study was to compare the N leaching loss and pasture N uptake from autumn-applied dairy shed effluent and ammonium fertilizer (NH4Cl) labeled with 15N, using intact soil lysimeters (80 cm diameter, 120 cm depth). The soil used was a sandy loam, and the pasture was a mixture of perennial ryegrass (Lolium perenne) and white clover (Trifolium repens). The DSE and NH4Cl were applied twice annually in autumn (May) and late spring (November), each at 200 kg N ha-1. The N applied in May 1996 was labeled with 15N. The lysimeters were either spray or flood irrigated during the summer. The autumn-applied DSE resulted in lower N leaching losses compared with NH4Cl. However, the N applied in the autumn had a higher potential for leaching than N applied in late spring. Between 4.5–8.1% of the 15N-labeled mineral N in the DSE and 15.1–18.8% of the 15N-labeled NH4Cl applied in the autumn were leached within a year of application. Of the annual N leaching losses in the DSE treatments (16.0–26.9 kg N ha-1), a fifth (20.3–22.9%) was from the mineral N fraction of the DSE applied in the autumn, with the remaining larger proportion from the organic fraction of the DSE, soil N and N applied in spring. In the NH4Cl treatments, more than half (53.8–64.8%) of the annual N leaching loss (55.9–57.6 kg N ha-1) was derived from the autumn-applied NH4Cl. DSE was as effective as NH4Cl in stimulating pasture production. Since only 4.4–4.5% of the annual herbage N uptake in the DSE treatment and 12.3–13.3% in the NH4Cl treatment were derived from the autumn-applied mineral N, large proportions of the annual herbage N uptake must have been derived from the N applied in spring, the organic N fraction in the DSE, soil N and N fixed by clover. The recoveries of 15N in the herbage were similar between the DSE and the NH4Cl treatments, but those in the leachate were over 50% less from the DSE than from the NH4Cl treatment. The lower leaching loss of 15N in the DSE treatment was attributed to the stimulated microbial activities and increased immobilization following the application of DSE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004677902049
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    Combining ability of resistance to head blight caused by Fusarium culmorum (W.G. Smith) in the F1 of a seven parent diallel of winter wheat (Triticum aestivum L.) (1999)
    Springer
    Euphytica 110 (1999), S. 199-206 
    Details
    ISSN: 1573-5060
    Keywords: diallel analysis ; Fusarium culmorum ; heterosis ; resistance ; scab ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fusarium head blight (FHB, scab) caused by Fusarium spp. is a widespread disease of cereals causing relevant yield and quality losses and contaminating cereal products with mycotoxins. Breeding resistant cultivars is the method of choice for controlling the disease. Resistance to FHB is a quantitative trait and is most likely governed by several genes. We present the results of an F1 diallel analysis of FHB resistance involving six resistant and one susceptible European winter wheat genotypes of diverse origin in order to identify promising combinations for the selection of improved cultivars. Parents and F1s including reciprocals were evaluated for FHB resistance in an artificially inoculated field trial. Two traits were assessed: visual disease symptoms on the heads and the percentage of Fusarium damaged kernels in a harvested sample. General combining ability (GCA) and specific combining ability (SCA) effects were statistically significant for visual symptoms and kernel damage, whereas reciprocal effects were small or not significant. Heterosis for resistance was common, indicating that the parental genotypes possess different resistance genes. Selection of transgressive segregates should be feasible from such heterotic combinations.
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    URL: http://dx.doi.org/10.1023/A:1003757002052
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    Molecular mapping of a new genic male-sterility gene causing chalky endosperm in rice ( Oryza sativa L.) (1999)
    Springer
    Euphytica 106 (1999), S. 57-62 
    Details
    ISSN: 1573-5060
    Keywords: genic male-sterility gene ; mapping ; molecular markers ; Oryza sativa ; pleiotropic effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A genic male-sterility gene newly induced by chemical mutagenesis, tentatively designated as ms-h(t), was located on the molecular map of rice and tested for its effect on chalky endosperm. Bulked segregant analysis was used to determine the chromosomal location of the ms-h(t) locus by screening four to five RFLP markers per chromosome. After confirming that the gene was located on chromosome 9, twenty-four RFLP markers from chromosome 9 were surveyed for polymorphism in the parents of the mapping population. Of these, eleven markers were mapped around the ms- h(t) locus. RG451 and RZ404 flanked the ms-h(t) gene, at 2.5cM and 3.3cM, respectively. Heterozygous F2 to F4 progenies were tested for co- segregation of male-sterility and chalky endosperm and it was revealed that ms-h(t) might have a pleiotropic effect on chalky endosperm. This mutant would be a good biological material to characterize the biochemical mechanism of male sterility and related pleiotropic effects. Further studies should be needed to know the usefulness of this mutant for hybrid seed production.
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    Importance of ornithine-δ-aminotransferase to proline accumulation caused by water stress in detached rice leaves (1999)
    Springer
    Plant growth regulation 27 (1999), S. 191-194 
    Details
    ISSN: 1573-5087
    Keywords: ornithine-δ-aminotransferase ; proline accumulation ; Oryza sativa ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Proline is synthesized either from glutamate or from ornithine in plants. Relatively little is known about the contribution of the pathway from ornithine to proline biosynthesis. In this paper we investigated the contribution of ornithine-δ-aminotransterase (OAT), an enzyme responsible for ornithine pathway, to proline accumulation in water-stressed detached rice leaves. Although OAT activity increased with the increase of water stress duration, a pattern similar to that obtained for proline accumulation, the ornithine pathway in rice leaves seems to contribute little, if any, to proline accumulation under water stress condition. This conclusion was based on the observations that (a) gabaculine (50 μM), an inhibitor of OAT, inhibited about 75% OAT activity caused by water stress but reduced only 20% of proline content and (b) cycloheximide, a protein synthesis inhibitor, had no effect on OAT activity induced by water stress but significantly reduced proline accumulation.
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    URL: http://dx.doi.org/10.1023/A:1006226732574
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    Endogenous abscisic acid levels are not linked to methyl jasmonate-promoted senescence of detached rice leaves (1999)
    Springer
    Plant growth regulation 28 (1999), S. 17-20 
    Details
    ISSN: 1573-5087
    Keywords: abscisic acid ; ammonium ; leaf senescence ; methyl jasmonate ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Both abscisic acid (ABA) and jasmonates are known to promote leaf senescence. Since ABA and jasmonates have both chemical and physiological similarities, we are interested to know whether senescence of detached rice leaves induced by methyl jasmonate (MJ) is mediated through an increase in endogenous ABA levels. In darkness, the endogenous level of ABA in detached rice leaves remained unchanged in the first day of incubation in water and increased about 5 times its initial value in the second day. However, the pattern of senescence, as judged by protein loss, was rapid during the first day. MJ significantly promoted senescence of detached rice leaves. Contrary to our expectation, endogenous ABA levels decreased in MJ-treated detached rice leaves. Similar to the effect of MJ, endogenous ABA levels decreased in detached rice leaves which were induced to senesce by treatment with NH4Cl. These results suggest that endogenous ABA levels are not linked to MJ-induced senescence of detached rice leaves.
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    URL: http://dx.doi.org/10.1023/A:1006251922889
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    Induction by ozone of ethylene production and an ACC oxidase cDNA in rice (Oryza sativa L.) leaves (1999)
    Springer
    Plant growth regulation 28 (1999), S. 123-127 
    Details
    ISSN: 1573-5087
    Keywords: 1-aminocyclopropane-1-carboxylate (ACC) oxidase ; ethylene ; Oryza sativa ; ozone ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Exposure to ozone at 1 µl l−1 for 6 h induced ethylene production in rice (Oryza sativa L. cv. Hitomebore) leaves. The stimulation of ethylene production was detectable 2 h after the start of the exposure to ozone, and lasted for 6 h after the exposure. A 429-bp cDNA fragment encoding ACC oxidase was obtained by RT-PCR from ozone-treated rice leaves. Its nucleotide sequence and deduced amino-acid sequence had 97.2% and 94.4% identity, respectively, to those of OS1A1COX, which was previously obtained from deepwater rice. The abundance of the cDNA increased in accordance with the induction of ethylene production by the exposure to ozone.
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    URL: http://dx.doi.org/10.1023/A:1006229421785
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    First source of resistance in durum wheat to Hessian fly (Diptera: Cecidomyiidae) in Morocco (1999)
    Springer
    Genetic resources and crop evolution 46 (1999), S. 107-109 
    Details
    ISSN: 1573-5109
    Keywords: antibiois ; Mayetiola destructor ; resistance ; Triticum turgidum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hessian fly, Mayetiola destructor (Say), is the major pest of wheat in North Africa. In Morocco, durum wheat (Triticum turgidum L. subsp. durum (Desf). Husn.) losses due to this pest have been estimated at 32%. Genetic resistance is the only economical and practical means of controlling this insect. Field and greenhouse screening of durum wheat genotypes resulted in the identification of one source of resistance to Hessian fly in Morocco. This is the first source of durum wheat Hessian fly-resistance identified in Morocco. This source of resistance expresses a medium level of antibiosis against first-instar Hessian fly larvae; about 25% of the larvae survive on resistant plants. The deployment of varieties that allow for larval survival on resistant plants should reduce selection for biotype development. This source of resistance is being used by CIMMYT/ICARDA and Moroccan breeders to develop resistant durum wheat varieties.
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    URL: http://dx.doi.org/10.1023/A:1008615515704
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    High-resolution DNA fingerprinting of Indian rice (Oryza sativa L) varieties by amplified fragment length polymorphism (1999)
    Springer
    Genetic resources and crop evolution 46 (1999), S. 427-433 
    Details
    ISSN: 1573-5109
    Keywords: AFLP analysis ; DNA fingerprinting ; genetic diversity ; Indian rice varieties ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To assess diversification of indica rice, fingerprinting of 42 Indian rice varieties (Oryza sativa L) and one accession of O. brachyantha Chev. et Roehr. was taken up using amplified fragment length polymorphism (AFLP). Six primer combinations of PstI and MseI were used for this purpose. On denaturing polyacrylamide sequencing gel, on average 41 scorable AFLP bands were obtained of which 81% were polymorphic. Each variety revealed a distinct fingerprint. Data were scored on the basis of presence and absence of bands and cluster analysis was performed using Dice similarity coefficients. The resultant dendrogram showed four major clusters and traits. Some varieties especially landraces revealed more polymorphism and unique loci. Such study will be a valuable tool for proper choice of parents in mapping populations or breeding programs to produce heterotic several inexplicable minor clusters. Some varieties were grouped according to parentages of the cross while some were grouped according to physiological combinations apart from providing a characteristic fingerprint.
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    URL: http://dx.doi.org/10.1023/A:1008696306235
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    Geographical distribution of genes causing hybrid breakdown in varietal crosses of Asian cultivated rice (1999)
    Springer
    Genetic resources and crop evolution 46 (1999), S. 13-17 
    Details
    ISSN: 1573-5109
    Keywords: F2 weakness ; reproductive barrier ; Oryza sativa ; gene distribution ; clinal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a backcrossing program to introduce the wx (glutinous endosperm) gene from a Thai upland rice cultivar, Col.No.15, to a Japanese cultivar, Sasanishiki, of Asian cultivated rice, Oryza sativa L., weak plants were observed in the BC1F1 generation. These weak plants were characterized by poor growth and discoloration at the tillering stage, though they were completely fertile. Hybrid breakdown, which is defined as hybrid weakness and sterility detected in the F2 and later inbred generations of varietal crosses, is controlled by a pair of recessive genes, hwd1 and hwd2, at unlinked loci. Two dominant genes at either the same or different loci, Hwd1/Hwd1 hwd2/hwd2, hwd1/hwd1 Hwd2/Hwd2 or Hwd1/hwd1 Hwd2/hwd2, are needed for normal growth. Using tester lines homozygous for a pair of recessive genes selected in the BC1F3 generation, the genotypes for hybrid breakdown of 100 Asian rice cultivars were determined based on the phenotype of F1 plants. Clinal variation for hybrid breakdown was observed. Cultivars with two dominant alleles at either hwd1 or hwd2 locus, were mainly found in insular Asia (Japan, Philippines and Indonesia), while the frequency of cultivars with four dominant alleles was more common in cultivars from continental Asia. Roles of hybrid breakdown in genetic differentiation of Asian cultivated rice are discussed.
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    URL: http://dx.doi.org/10.1023/A:1008631600311
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    Restoration of gibberellin biosynthesis by 2,6-diisopropylphenoxyacetic acid in uniconazole-treated rice plants (1999)
    Springer
    Plant growth regulation 28 (1999), S. 67-72 
    Details
    ISSN: 1573-5087
    Keywords: 2,6-diisopropylphenoxyacetic acid ; gibberellin ; Oryza sativa ; rice ; shoot elongation ; uniconazole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract 2,6-Diisopropylphenoxyacetic acid (DIPA), a promoter of growth and flowering of Sagittaria species, was found to improve the shoot growth of rice plants treated with uniconazole, an inhibitor of gibberellin (GA) biosynthesis. In a modified micro-drop bioassay using semi-dwarf rice, Oryza sativa L. cv. Tan-ginbozu, in which uniconazole had been incorporated into the agar medium, a significant recovery from growth inhibition was observed for both the 3rd and the 4th leaf sheaths but not for the 2nd sheath. In greenhouse experiments, uniconazole-treated rice plants partially recovered from growth inhibition when DIPA was applied after uniconazole treatment, whereas DIPA applied with, or before, uniconazole treatment did not improve growth. The levels of GA1 and GA20 in the rice plants treated with uniconazole plus DIPA were almost equal to those of the untreated controls, indicating that the observed growth recovery is attributable to the restoration of GA biosynthesis by DIPA.
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    URL: http://dx.doi.org/10.1023/A:1006215428363
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    Mapping QTLs associated with drought resistance in rice: Progress, problems and prospects (1999)
    Springer
    Plant growth regulation 29 (1999), S. 123-133 
    Details
    ISSN: 1573-5087
    Keywords: Oryza sativa ; drought resistance ; root morphology ; root growth ; osmotic adjustment ; review
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The use of molecular markers in the mapping of traits of agronomic importance holds great promise for speeding the development of improved plant varieties and increasing our understanding of the physiological or molecular mechanisms behind biological phenomena. The technique is now being applied to drought resistance in rice (Oryza sativa L.). Drought is important because a considerable proportion of the world rice area is not irrigated and can be prone to water deficit. A large number of people, particular some of the poorest rice farmers, stand to gain if new varieties which combine high yield and drought resistance can be developed. Rice should be particularly useful for the molecular genetic analysis of drought resistance because of its growing role as a model monocot species and the diversity of drought resistance mechanisms which are found in the germplasm. We briefly review the traits which might be considered important in improving drought resistance in rice, before explaining the molecular mapping approach. We review progress at locating quantitative trait loci (QTLs) for individual mechanisms of drought resistance in controlled environment conditions. This includes a detailed comparison of reported QTLs related to root morphological characters. The search for QTLs associated with field performance under drought stress is analysed and the problems associated with understanding the genetic control of a complex physical and physiological phenomenon under conditions of substantial environmental variation are highlighted. We emphasise that the use of near isogenic lines in overcoming some of the problems offers considerable promise for the future.
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    URL: http://dx.doi.org/10.1023/A:1006255832479
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    Resistance to foliar blight of wheat: A search (1999)
    Springer
    Genetic resources and crop evolution 46 (1999), S. 469-475 
    Details
    ISSN: 1573-5109
    Keywords: foliar blight ; germplasm ; resistance ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract One thousand three hundred and eighty-seven spring wheat germplasm (Triticum aestivum L.) lines belonging to the Indian and CIMMYT wheat programmes were evaluated for their tolerance to foliar blight disease for three consecutive years i.e., from 1994 to 1997. Disease severity at six different growth stages, beginning from tillering to late milk stage, was recorded. None of the genotypes showed immunity to the disease. Of 43 lines showing resistant reaction, a major proportion (25) was represented by CIMMYT material. Comparatively, Indian germplasm lines tended to be more susceptible at more advanced growth stages. Area Under Disease Progress Curve (AUDPC) and Apparent Infection Rate (r) values of resistant lines were much lower than those of susceptible ones, but lower AUDPC in some of the resistant lines did not correspond to a lower 'r' value. Most of the resistant lines were derived from Seri, Myna, Bau, kauz, Hork 's' and Aegilops tauschii Coss.
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    URL: http://dx.doi.org/10.1023/A:1008797232108
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    Resistance to Meloidogyne javanica in wild Arachis species* (1999)
    Springer
    Genetic resources and crop evolution 46 (1999), S. 557-568 
    Details
    ISSN: 1573-5109
    Keywords: Arachis spp. ; Meloidogyne javanica ; resistance ; root-knot nematodes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The root-knot nematode, Meloidogyne javanica Race 3 is an important nematode parasite of groundnut. Greenhouse evaluation of 184 accessions of 33 wild Arachis spp. five interspecific derivatives, 18 groundnut cultivars for root damage (galls formed by nematode) and nematode reproduction demonstrated that resistance to the nematode is available in the genepool of wild Arachis spp. Seven accessions, ICG 8952 (Arachis helodes), ICC 13211 (A. sylvestris), ICG 13224 (A. kretscmeri), ICG 13231 (Arachis sp.), ICG 14862 (A. kuhlmannii), ICG 14868 (A. stenosperma), and ICG 14915 (A. sylvestris) were highly resistant to nematode reproduction and root damage. There was no gall and eggmass formation on any plant of these accessions. Thirty-three accessions were resistant and 14 were moderately resistant. All the tested accessions of A. monticola, A. benensis, A. ipaensis, A. hoehnei, A. kempff-mercadoi, A. valida, A. chiquitana, A. rigonii, A. vallsii, A. dardani, A. paraguariensis, A. triseminata, interspecific derivatives, and groundnut cultivars were susceptible. The possible use of resistance sources in the breeding program is discussed.
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    URL: http://dx.doi.org/10.1023/A:1008754812257
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    The genetic structure and conservation of aus, aman and boro rices from Bangladesh (1999)
    Springer
    Genetic resources and crop evolution 46 (1999), S. 587-598 
    Details
    ISSN: 1573-5109
    Keywords: diversity ; genetic resources ; GIS ; Oryza sativa ; RAPD ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A diverse set of 115 rice varieties from Bangladesh was surveyed using 35 polymorphic RAPD (randomly amplified polymorphic DNA) markers and the genetic structure of this germplasm, encompassing the principal rice ecotypes of Bangladesh (aus, aman and boro), was determined using multivariate analysis. The level of genetic diversity was evaluated and compared with the levels of diversity found within other rice growing areas of the world. Geographical information systems analysis using Atlas-GIS was employed to analyse and present the geographic distribution of genetic diversity across Bangladesh, and cluster analysis was used to test the efficiency of selection of material for a core collection.
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    URL: http://dx.doi.org/10.1023/A:1008749532171
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    Estimation of the Importance of Spatially Variable Nitrogen Application and Soil Moisture Holding Capacity to Wheat Production (1999)
    Springer
    Precision agriculture 1 (1999), S. 27-38 
    Details
    ISSN: 1573-1618
    Keywords: CERES ; wheat ; soil moisture ; nitrogen ; variability ; precision agriculture ; spatial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Crop growth modelling techniques were used to investigate the performance of a wheat crop over a range of weather conditions, nitrogen application rates and soil types. The data were used to predict long term benefits of using spatially variable fertilizer application strategies where fertilizer application rate was matched to the soil type, against a strategy of uniform fertilizer application. The model was also run with modified soil properties to determine the importance of soil moisture holding capacity in the variability of crop yield. It was found that the benefits of spatially variable nitrogen management when fertilizer was applied at the beginning of the season were modest on average. The range of results for different weather conditions was much greater than the average benefit. A large proportion of the variability of crop performance between soil types could be explained by differing soil moisture holding capacity. Devising techniques for managing this variability was concluded to be important for precision farming of cereals.
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    Nitrogen utilization from diluted and undiluted nitric acid treated cattle slurry following surface application to grassland (1999)
    Springer
    Nutrient cycling in agroecosystems 53 (1999), S. 269-280 
    Details
    ISSN: 1573-0867
    Keywords: acidification ; ANE ; ANR ; calcium ammonium nitrate ; cattle slurry ; dilution ; grassland ; nitric acid ; nitrogen ; residual effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Acidification of cattle slurry with nitric acid to pH 4.5 was studied on experimental dairy farms in the Netherlands as a measure to reduce ammonia losses. This paper presents the results of two field experiments, carried out to assess the utilization of nitrogen (N) from nitric acid treated cattle slurry following surface application to grassland. Three aspects were studied: (i) the utilization of N from nitric acid treated cattle slurry following a single surface application to grassland; (ii) the effects of multiple applications of nitric acid treated cattle slurry on N utilization, residual inorganic N in the soil and sward quality; and (iii) the residual effects of nitric acid treated slurry on DM and N yield. In these experiments undiluted acidified slurry (UAS) and diluted acidified slurry (DAS) were compared with calcium ammonium nitrate (CAN). Apparent N recoveries (ANR) and apparent N efficiencies (ANE) were calculated on the basis of inorganic N in applied fertilizer and slurries. Following single applications of UAS and CAN on the sward on the clay soil, the average ANR values of UAS and CAN were 0.74 and 0.75 kg kg-1, respectively. The average ANE values of UAS and CAN were 30.3 and 29.5 kg DM per kg N, respectively. Single applications of DAS and CAN on the sandy soil resulted in average ANR values of 0.65 and 0.84 kg kg-1 and average ANE values of 24.1 and 29.2 kg DM per kg N for DAS and CAN, respectively. On average, the direct ANR values after four successive applications of CAN, UAS or DAS were 0.82, 0.82 and 0.74 kg kg-1, respectively and the direct ANE values were 28.4, 27.8 and 27.0 kg DM per kg N. On the sandy soil, the amount of residual inorganic N in the soil after the 4th cut increased with increasing rates of inorganic N application, with a slightly higher amount on plots treated with acidified slurry than on plots fertilized with CAN. On the clay soil, the amount of residual inorganic N was not affected by N application rate nor N source. On average, the residual ANR values, in the unfertilized 5th and 6th cuts, of CAN, UAS or DAS were 0.08, 0.10 and 0.09 kg kg-1, respectively and the residual ANE values were 2.7, 3.3 and 3.0 kg DM per kg N. It was concluded that nitric acid treated cattle slurry is an effective N fertilizer on grassland and that the residual effect of nitric acid treated cattle slurry is only marginally higher than that of CAN. Repeated applications of nitric acid treated cattle slurry did not affect sward composition.
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    Growth dynamics of reed canarygrass ( Phalaris arundinacea L.) and its allocation of biomass and nitrogen below ground in a field receiving daily irrigation and fertilisation (1999)
    Springer
    Nutrient cycling in agroecosystems 54 (1999), S. 21-29 
    Details
    ISSN: 1573-0867
    Keywords: carbon balance ; daily irrigation and fertilisation ; minirhizotrons ; nitrogen ; Phalaris arundinacea ; roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Biomass and nitrogen in the roots, rhizomes, stem bases and litter of reed canarygrass (Phalaris arundinacea L.) were repeatedly estimated by soil coring, and root growth dynamics of this potential energy crop was studied for two years using minirhizotrons. Results are discussed in relation to above-ground biomass and nitrogen fertilisation. Five treatments were used: C0, unfertilised control; C1, fertilised with solid N fertiliser in spring; I1, irrigated daily, fertilised as in C1; IF1 , irrigated as I1 and fertilised daily through a drip-tube system; IF2, as in IF1 but with higher N fertiliser rates. Biomass of below-ground plant parts of reed canarygrass increased between the first and second years. Up to 50% of total plant biomass and nitrogen were recovered below-ground. The highest proportions were found in C0. The calculated annual input via root turnover ranged between 80 and 235 g m-2. In absolute terms, up to 1 kg and 10 g m-2 of biomass and nitrogen, respectively, were found in below-ground plant fractions. High inputs of stubble and accumulated below-ground biomass will occur when the ley is ploughed, which will result in a highly positive soil carbon balance for this crop in comparison with that of conventional crops such as cereals.
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    URL: http://dx.doi.org/10.1023/A:1009701422394
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    Effect of Rhizobium inoculation and nitrogen fertilization on yield and protein content of six chickpea (Cicer arietinum L.) cultivars in marginal soils under irrigation (1999)
    Springer
    Nutrient cycling in agroecosystems 54 (1999), S. 57-63 
    Details
    ISSN: 1573-0867
    Keywords: chickpea ; marginal soils ; nitrogen ; protein content ; Rhizobium ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was carried out for two consecutive seasons 1994/95 and 1995/96 at ElRwakeeb (a sandy clay loam) to study the effect of Rhizobium sp. (Cicer) inoculation and N fertilization on six chickpea cultivars (Baladi, Gabel marra, NEC 25–27, NEC 2010, ILC 1919, and Flip 85–108). Plants were either inoculated with three Rhizobium sp. (Cicer) strains (TAL 480, TAL 620 and TAL 1148) separately, or N fertilized (50 kg N ha-1). The results of the two seasons indicated the absence of infective strains for chickpea in the soil. Rhizobium inoculation or N fertilization significantly increased the total nodule number per plant, 100 seed weight, yield and protein content of seeds. The results indicated that the three Rhizobium strains are infective and effective in nitrogen fixation. Inoculation with Rhizobium strain TAL 1148 resulted in a significant increment in most of the parameters studied, compared to other strains and untreated control. Cultivar ILC 1919 was the best yielding cultivar, whereas, cultivar NEC 2010 contained the highest protein content, however cultivar Gabel marra showed the highest amount of protein due to inoculation or N fertilization, in the two seasons. Inoculation with Rhizobium strain TAL 1148 increased yield by 72 and 70%, whereas, 50 kg N ha-1 increased it by 70 and 69% in the first and second seasons, respectively. The amounts of protein accumulated (kg ha-1) due to N or Rhizobium inoculation were determined for all cultivars. The results obtained from the inoculation were comparable to those of 50 kg N ha-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009778727102
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  • 94
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    Nutrient cycling and growth in forest ecosystems of south western Australia: Relevance to agricultural landscapes (1999)
    Springer
    Agroforestry systems 45 (1999), S. 215-244 
    Details
    ISSN: 1572-9680
    Keywords: biodiversity ; disturbance ; nitrogen ; phosphorus ; resilience ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nutrient and hydrologic cycles in harvested native forests in southern Australia are largely balanced. For example, we have little or no evidence of any decline in nutrient capital or availability in harvested forests. Short-term and small-scale reductions in evapotranspiration due to loss of leaf area after harvesting are adequately balanced at the landscape scale by large areas of regenerating or older-age forest. In contrast, agricultural systems on similar soils are a) dependent on large inputs of fertilisers to maintain growth and b) frequently subject to increasing salinity and waterlogging or other forms of degradation. The large-scale replacement of long-lived communities of perennial and often deep- rooting native species with annual crops or other communities of shallow-rooting species might be better managed within the framework of knowledge developed from studies of native plant communities. However, application of such a mimic concept to systems of low natural productivity is limited when agricultural systems require continued high productivity. Nonetheless, the mimic concept may help in developing sustainable management of agriculture on marginal lands, and contribute to the nutritional resilience of agroecosystems. Relevant characteristics for mimic agroecosystems in south western Australia include: high species diversity, diversity of rooting attributes, utilisation of different forms of nutrients (especially of N and P) in space and time, and the promotion of practices which increase soil organic matter content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006267604313
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  • 95
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    Effect of nitrogen and phosphorus addition on phenanthrene biodegradation in four soils (1999)
    Springer
    Biodegradation 10 (1999), S. 43-50 
    Details
    ISSN: 1572-9729
    Keywords: biodegradation ; nitrogen ; nutrients ; phenanthrene ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Phenanthrene mineralization rates were found to vary widely among four soils; differences in soil nutrient levels was one hypothesis to explain this variation. To test this hypothesis, phenanthrene mineralization rates were measured in these soils with, and without, added nitrogen and phosphorus. Mineralization rates either remained unchanged or were depressed by the addition of nitrogen and phosphorus. Phenanthrene degradation rates remained unchanged in the soil which had the highest indigenous levels of nitrogen and phosphorus and which showed the largest increase in phosphorus levels after nutrients were added. The soils in which degradation rates were depressed had lower initial phosphorus concentrations and showed much smaller or no measurable increase in phosphorus levels after nutrients were added to the soils. To understand the response of phenanthrene degradation rates to added nitrogen and phosphorus, it may be necessary to consider the bioavailability of added nutrients and nutrient induced changes in microbial metabolism and ecology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008359606545
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  • 96
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    Soil community composition and ecosystem processes: Comparing agricultural ecosystems with natural ecosystems (1999)
    Springer
    Agroforestry systems 45 (1999), S. 159-185 
    Details
    ISSN: 1572-9680
    Keywords: decomposition ; environmental monitoring ; fertiliser ; mineralisation ; nitrogen ; pesticides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil organisms play principal roles in several ecosystem functions, i.e. promoting plant productivity, enhancing water relations, regulating nutrient mineralisation, permitting decomposition, and acting as an environmental buffer. Agricultural soils would more closely resemble soils of natural ecosystems if management practices would reduce or eliminate cultivation, heavy machinery, and general biocides; incorporate perennial crops and organic material; and synchronise nutrient release and water availability with plant demand. In order to achieve these goals, research must be completed to develop methods for successful application of organic materials and associated micro-organisms, synchronisation of management practices with crop and soil biota phenology, and improve our knowledge of the mechanisms linking species to ecosystem processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006299100678
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  • 97
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    The evidence for the extent of N.I. Vavilov's new world Andean centres of cultivated plant origins (1999)
    Springer
    Genetic resources and crop evolution 46 (1999), S. 163-168 
    Details
    ISSN: 1573-5109
    Keywords: Vavilov ; centres of diversity ; America ; Solanium ; potatoes ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Vavilov's ideas on New World centres of crop plant diversity are reviewed and the development of his ideas from 1926 to 1940 are described. More recent collecting work and taxonomic studies by the author confirm largely the delimitation of the American gene centres suggested by Vavilov, even in regard to details, e.g. within Bolivia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008642630144
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  • 98
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    Inheritance and allelism for resistance to Russian Wheat Aphid in an Iranian Spring Wheat (1999)
    Springer
    Euphytica 107 (1999), S. 71-78 
    Details
    ISSN: 1573-5060
    Keywords: Russian wheat aphid ; resistance ; inheritance ; allelism ; segregation ratio ; wheat ; Triticum aestivum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), is an important pest of wheat (Triticum aestivum L.) in the United States of America. Developing adapted wheat cultivars with genetic resistance to RWA is an effective control strategy. Genetic studies were conducted to determine the mode of inheritance of gene(s) conferring resistance to RWA in an Iranian landrace wheat line, G 5864. For the inheritance study, G 5864 was crossed with the susceptible wheats ‘Yecora Rojo’ and ND 2375. Seedlings of F1, reciprocal F1, F2, BC1 to the susceptible parent (BCS), and BC1 to the resistant parent (BCR) were screened for RWA reaction. Several phenotypic segregation ratios were tested in the F2 populations for goodness of fit; the 9:3:3:1 ratio (resistant: rolled leaves: stunted plants: susceptible) was an acceptable fit in all cases. Thus, resistance in G 5864 seemed to be controlled by two independent dominant genes with additive gene effects. The allelic relationships of gene(s) in this line with genes in other resistant lines, PI 137739 (Dn1), PI 262660 (Dn2), PI 372129 (Dn4), PI 294994 (Dn5), and PI 243781 (Dn6), were also studied. Segregation patterns observed in G 5864 × resistant (R × R) F2 populations were inconclusive. However, no susceptible plants were observed in these F2 populations. If previous reports concerning the number of resistance genes present in the other resistant lines are correct, then given the high manifestation of resistance observed in G 5864, and given the absence of susceptible plants in the R × R F2 populations, it is indicated that RWA resistance in G 5864 is either controlled by different alleles at the same loci as the other resistance genes, or that G 5864 shares a resistance gene with each of the other resistant lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003549512216
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  • 99
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    Heading date and resistance to septoria tritici blotch in wheat not genetically associated (1999)
    Springer
    Euphytica 106 (1999), S. 63-68 
    Details
    ISSN: 1573-5060
    Keywords: disease assessment ; maturity ; resistance ; Septoria tritici ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nineteen cultivars, with large differences in heading date, were evaluated for their response to septoria tritici blotch in two experimental setups in Njoro, Kenya. Due to the more or less constant temperatures during the growing season and the overhead irrigation applied the epidemic conditions were similar over the whole observation period for the early and late cultivars. In experiment 1 the cultivars were assessed for disease severity at the same moment irrespective of the developmental stage, while in experiment 2 the cultivars were assessed at the same developmental stage. Measured at the same time, the disease severity was highest in the early maturing cultivars and lowest in the late maturing cultivars (r = –0.78). When assessed at the same development stage the disease build up was independent of heading date (r = –0.10) but strongly dependent on resistance level. There were no indications that early heading cultivars were more susceptible than late heading cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003593218761
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  • 100
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    A root-injection method to assess verticillium wilt resistance of peppermint ( Mentha × piperita L.) and its use in identifying resistant somaclones of cv. Black Mitcham (1999)
    Springer
    Euphytica 106 (1999), S. 223-230 
    Details
    ISSN: 1573-5060
    Keywords: Mentha piperita ; peppermint ; resistance ; somaclone ; verticillium wilt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three studies were initially conducted using five mint cultivars with known disease reactions to verticillium wilt to determine if inoculation method, root-dip as compared to root-injection, had an affect on the plant growth and disease reaction. The planting media did not affect the development of wilt symptoms in susceptible varieties, however, the soil/peat based medium resulted in higher shoot fresh weight at harvest than the perlite/vermiculite medium. Black Mitcham had the highest susceptible disease rating and greatest reduction in fresh shoot weight from inoculation with Verticillium dahliae at two inoculum concentrations, 104 or 106 microconidia/ml. Native spearmint was resistant and Mentha crispa was moderately resistant, whereas, Murray Mitcham peppermint and Scotch spearmint were moderately susceptible to verticillium wilt with a corresponding lower fresh shoot weight. Scotch spearmint was less susceptible at the low inoculum concentration than at a higher level of microconidia, indicating a low level of disease resistance. Overall, the two inoculation methods resulted in similar cultivar responses to verticillium wilt, although the root-injection method was more applicable for large plant populations. Thus, the root-injection method of inoculation was utilized to screen 743 Black Mitcham derived somaclones for wilt resistance, of which nine somaclones were found resistant in repeated inoculation tests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003591908308
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