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Agro-morphological characterization of ovary culture-derived plants of rice (Oryza sativa L.)

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Abstract

Plants derived from unpollinated ovary culture of ten rice genotypes showed significant variability in agro-morphological characteristics. The ovary-derived plant (H1) populations were completely haploid, doubled haploid or haploid-doubled haploid mixture. Haploids had very drastic reduction in plant height, panicle length, grain length, breadth and number and spikelet fertility (0.0%–2.1%). Doubled haploids from the hybrid of UPRI 95–121 × UPRI 95–165 were normal with fertility ranging between 69.6% and 97.7%. A genetic segregation in ratio of 1:1 was observed for five pigmentation characters in the H1 population derived from hybrid UPRI 95–122 × UPRI 95–165. Plant height showed the largest coefficient of variability (28.5%) followed by the number of spikelets per panicle (24.2%), number of grains per panicle (22.0%), percent seed set (9.2%) and panicle length (9.0%). The range of variation in the H1 population from fully fertile hybrid PMS 2A (CMS) × IR 31802 (restorer) was similar to its corresponding F2 population for plant height, spikelet fertility and number of grains/panicle. A single clone of plants from the cultivar BG 1321 exhibited complete male sterility but normal female fertility when pollinated with other varieties. Ovary-derived plants from the CMS lines PMS 2A and IR 58025A showed stable male sterility and those from thermosensitive genetic male sterile line UPRI 95–140 showed thermosensitive genetic male sterility. These lines have potential in the hybrid breeding program and are being currently exploited.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, G.B. Plant University of Agriculture and Technology, Pantnagar-, 263 145, India

    Li Rongbai, M.P. Pandey & S.K. Pandey

  2. Department of Genetics and Plant Breeding, G.B. Plant University of Agriculture and Technology, Pantnagar-, 263 145, India

    D.K. Dwivedi

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  1. Li Rongbai
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  2. M.P. Pandey
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  3. S.K. Pandey
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  4. D.K. Dwivedi
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Rongbai, L., Pandey, M., Pandey, S. et al. Agro-morphological characterization of ovary culture-derived plants of rice (Oryza sativa L.). Euphytica 106, 197–203 (1999). https://doi.org/10.1023/A:1003524217816

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  • DOI: https://doi.org/10.1023/A:1003524217816

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