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  • 1
    Electronic Resource
    Electronic Resource
    Agro-morphological characterization of ovary culture-derived plants of rice (Oryza sativa L.) (1999)
    Springer
    Euphytica 106 (1999), S. 197-203 
    Details
    ISSN: 1573-5060
    Keywords: doubled haploids ; genetics ; haploids ; induced variation ; Oryza sativa ; ovary culture ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plants derived from unpollinated ovary culture of ten rice genotypes showed significant variability in agro-morphological characteristics. The ovary-derived plant (H1) populations were completely haploid, doubled haploid or haploid-doubled haploid mixture. Haploids had very drastic reduction in plant height, panicle length, grain length, breadth and number and spikelet fertility (0.0%–2.1%). Doubled haploids from the hybrid of UPRI 95–121 × UPRI 95–165 were normal with fertility ranging between 69.6% and 97.7%. A genetic segregation in ratio of 1:1 was observed for five pigmentation characters in the H1 population derived from hybrid UPRI 95–122 × UPRI 95–165. Plant height showed the largest coefficient of variability (28.5%) followed by the number of spikelets per panicle (24.2%), number of grains per panicle (22.0%), percent seed set (9.2%) and panicle length (9.0%). The range of variation in the H1 population from fully fertile hybrid PMS 2A (CMS) × IR 31802 (restorer) was similar to its corresponding F2 population for plant height, spikelet fertility and number of grains/panicle. A single clone of plants from the cultivar BG 1321 exhibited complete male sterility but normal female fertility when pollinated with other varieties. Ovary-derived plants from the CMS lines PMS 2A and IR 58025A showed stable male sterility and those from thermosensitive genetic male sterile line UPRI 95–140 showed thermosensitive genetic male sterility. These lines have potential in the hybrid breeding program and are being currently exploited.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003524217816
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  • 2
    Electronic Resource
    Electronic Resource
    Heterosis in inter and intrasubspecific crosses over three-environments in rice (1998)
    Springer
    Euphytica 99 (1998), S. 155-165 
    Details
    ISSN: 1573-5060
    Keywords: heterosis ; heterosis × environment interaction ; Oryza sativa ; rice ; standard heterosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Moderate to high heterosis for yield and ten related characters was studied in 45 crosses involving six indica (I) and four tropical japonica (J) varieties of rice under three (E1-optimum sowing and high fertility, E2-both optimum sowing and fertility and E3- late sowing and high fertility) environments. Trends of magnitude of heterosis for grain yield and plant height were I/J 〉 I/I 〉 J/J and for days to 50% flowering I/J 〉 J/J 〉 I/I hybrids. Estimates of standard heterosis (%) for grain yield were –64.5 to 146.1 in E1, –70.4 to 82.2 in E2 and –67.2 to 63.8 in E3. Environment E1 seemed to be more favorable for higher heterosis expression than others. Higher heterosis in yield also accompanied heterosis in panicle number, dry matter and spikelet and grain number per panicle. Most estimates for days to flower were negative. Heterotic I/J hybrids also recorded maximum heterosis for earliness. Moderate to low standard heterosis for plant height across environments (2.0 to 13.7%) was recorded. Hybrids were identified in specific environments for direct exploitation in hybrid breeding. Hybrids B4116 / Sarjoo 52, B4122 / Pant Dhan 4 and B4122 / Narendera 359 were more stable than others over three environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018357300923
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  • 3
    Electronic Resource
    Electronic Resource
    Development of a technique for in vitro unpollinated ovary culture in rice, Oryza sativa L. (1998)
    Springer
    Euphytica 104 (1998), S. 159-166 
    Details
    ISSN: 1573-5060
    Keywords: unpollinated ovary culture ; callus induction ; plant regeneration ; chilling pretreatment ; development stage ; medium composition ; rice ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A simple and efficient technique for in vitro unpollinated ovary culture in rice which is also applicable for indica genotypes was developed for breeding and genetic studies. Sampling explants at the auricle distance of 7–12 cm between the two uppermost leaves of a tiller, providing a chilling pretreatment and ovaries with 1/3 of the hulls intact gave optimum response to culture. For callus induction with the spontaneous breaking of ovaries, N6 media supplemented with NAA (2 mg/l) and DMSO (0.6–0.8%) gave a mean PCI value of 3.8% and range of 0.8–12.5% among genotypes. Media combining 2,4,5-T or 2,4-D with NAA in N6 medium also has reasonably good callus induction. For calli induced inside, 2,4-D (0.2–0.5 mg/l), NAA (2 mg/l) and KT (1 mg/l) contained media were superior. The maximum green plant regeneration (PPR) of 77.3% was found with the medium containing NAA 0.25 mg/l, IAA 0.5 mg/l and KT 2.0 mg/l. Significant genotype, medium and their interaction effects for per cent ovary survival and callus induction were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018613121306
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