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Identification of segregation-distortion-neutral alleles to improve pollen fertility of indica-japonica hybrids in rice (Oryza sativa L.)

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Abstract

Utilization of the pronounced heterosis of indica-japonica hybrids in rice had been difficult due to panicle sterility caused by male and female gamete abortion in such hybrids. But the female gamete abortion in the most indica-japonicahybrids has been solved by using an abortion-neutral gene S-5 n called as wide compatibility gene. On the other hand, the problem of pollen sterility remains to be solved for such hybrids. This study was conducted to identify abortion-neutral genes for pollen that may be utilized to alleviate pollen sterility in distant crosses. Some cultivars like Ketan Nangka (KN) and Dular, which are known as wide compatibility variety (WCV), were tested to find whether they possess neutral alleles for segregation distortion of marker genes. The distorted segregation of markers as well as normal segregation were confirmed among progeny lines of three-way crosses, WCV/indica//japonica and japonica//indica/WCV. Neutral alleles for segregation distortion were identified at two gamete gene loci (ga), i.e., ga-14(t) on chromosome 3 and ga-11 on chromosome 7. A new locus ga-14(t) was identified in between Pgi-1 and bc-1 and independent of ga-3 on chromosome 3. The neutral alleles at the two loci may be used to solve pollen sterility in indica-japonica hybrids.

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Authors and Affiliations

  1. Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    C.G. Lu

  2. Faculty of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    K. Takabatake & H. Ikehashi

Authors
  1. C.G. Lu
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  2. K. Takabatake
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  3. H. Ikehashi
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Lu, C., Takabatake, K. & Ikehashi, H. Identification of segregation-distortion-neutral alleles to improve pollen fertility of indica-japonica hybrids in rice (Oryza sativa L.). Euphytica 113, 101–107 (2000). https://doi.org/10.1023/A:1003929312252

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  • DOI: https://doi.org/10.1023/A:1003929312252

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