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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Agriculture and human values 17 (2000), S. 125-139 
    ISSN: 1572-8366
    Schlagwort(e): Environment ; Fisheries ; Fordism ; Nation-State ; Regulation ; Supranational State ; Transnational corporations ; Transnational State
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract This analysis uses an analytical frameworkgrounded in political economy perspectives of theglobalization of the agro-food sector combined with acase study approach focusing on the Marine StewardshipCouncil (MSC) to inform discussions regarding thecharacteristics of societal regulation in thepost-Fordist era. More specifically, this analysisuses the case of the emergence of the MSC toinvestigate propositions regarding the existence of,and location of, nascent forms of a transnationalState. The MSC proposes to regulate the certificationof sustainable fisheries at the global level throughan eco-labeling program. The MSC was created in 1996by the transnational environmental organization theWorld Wildlife Fund and the transnational corporationUnilever. The emergence of the MSC has generatedheated discussion in fisheries management circles thatis in general divided along North/South lines. Thisanalysis indicates that the case of the MSC providesvaluable insights into the possible characteristics ofsupranational regulatory mechanisms that might emulatethe role of the nation-State in the post-Fordist era.
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 57 (2000), S. 75-82 
    ISSN: 1573-0867
    Schlagwort(e): nitrogen ; leaching ; paddy soil ; wheat ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Nitrogen in percolation water was observed in paddy field soil under rice/wheat rotation. Different N-application rates were designed. Porous pipes were installed in triplicate at depths of 30, 60 and 90 cm to collect the water in the period of wheat growth. Suction cups were installed in triplicate at the same depths to collect the water during the period of rice growth. NH4 +, NO3 - and total N in the water were analysed with a continuous-flow nitrogen analyzer. Results showed that nitrate was the predominant form of nitrogen in percolation water during the period of wheat growth. Nitrate leaching was high in early spring after the `tillering fertilisation'. More than 50 mg l-1 of nitrate concentration in percolation water was observed for 30 and 60 cm in depth and more than 15 mg l-1 were observed for 90 cm. The concentration decreased quickly and was very low, less than 2 mg l-1 usually, in the earring stage of wheat. Nitrate in water was low, less than 1.5 mg l-1 usually, when the field was flooded during the period of rice growth. Some soluble organic N existed in the water. Nitrate in percolation water increased when the field was drained. The leaching loss of nitrogen during winter wheat growth period was estimated to be about 3.4% of the N-fertiliser applied at the normal application rate of farmers; for the rice growth period it was around 1.8%. Although a reduced N-application decreased N leaching, it caused a marked decrease in crop yield.
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 57 (2000), S. 171-181 
    ISSN: 1573-0867
    Schlagwort(e): boron fertilization ; rape ; rice ; residual effect
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Long-term field experiments (3–4 years) were conducted to evaluate the residual effect of boron (B) fertilizer for oilseed rape (Brassica napus L.) in an intensive crop rotation including two rice (Oryza sativa) crops per year. Experiments were conducted on four sites where the soil types were sandy, silty and clayey Inceptisols, and an Ultisol, located in the Zhejiang Province, Southeast China. Application of B fertilizer at rates of 1.1, 1.65 and 3.3 kg B/ha in the first year showed a different residual effect on oilseed yield in successive years, but had only small positive effects on the rice grain yield at two sites. The residual effect of 1.1 kg B/ha remained fully effective in correcting B deficiency in oilseed rape for 2 years in the Inceptisols, whereas the residual effect of 1.65 kg B/ha continued to correct B deficiency for at least 3 years in both the Inceptisols and the Ultisol. Foliar application of B fertilizer generally corrected B deficiency for oilseed rape but showed limited residual effect in the following years after application. The decline in residual values of B from a single fertilizer addition was closely related to the soil and leaf B concentration. Soil available B also decreased dramatically with the advance of rotation, but a larger decrease was found at a depth of 20–40-cm for the Inceptisols and the Ultisol. Thus, a more detailed understanding of the B cycling in the system is now needed to optimize management of B fertilizer.
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 58 (2000), S. 141-159 
    ISSN: 1573-0867
    Schlagwort(e): methane ; rice ; Oryza sativa ; anaerobic ; model ; simulation ; carbon dynamics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The development of the MERES (Methane Emissions in Rice EcoSystems) model for simulating methane (CH4) emissions from rice fields is described. The CERES-Rice crop simulation model was used as a basis, employing the existing routines simulating soil organic matter (SOM) decomposition to predict the amount of subsrate available for methanogenesis. This was linked to an existing submodel, described elsewhere in this volume (Arah & Kirk, 2000), which calculates steady-state fluxes and concentrations of CH4 and O2 in flooded soils. Extra routines were also incorporated to simulate the influence of the combined pool of alternative electron acceptors in the soil (i.e., NO3 −, Mn4+, Fe3+, SO4 2−) on CH4 production. The rate of substrate supply is calculated in the SOM routines of the CERES-Rice model from (a) the rate of decomposition of soil organic material including that left from the previous crop and any additions of organic matter, (b) root exudates (modified from the original CERES-Rice model using recent laboratory data), and (c) the decomposition of dead roots from the current crop. A fraction of this rate of substrate supply, determined by the concentration of the oxidized form of the alternative electron acceptor pool, is converted to CO2 by bacteria which outcompete the methanogenic bacteria, thereby suppressing CH4 production. Any remaining fraction of the substrate supply rate is assumed to be potentially available for methanogenesis. The CH4 dynamics submodel uses this potetial methanogenesis rate, along with a description of the root length distribution in the soil profile supplied by the crop model, to calculate the steady-state concentrations and fluxes of O2 and CH4. The reduced form of the alternative electron acceptor pool is allowed to reoxidize when soil pores fill with air if the field is drained. The MERES model was able to explain well the seasonal patterns of CH4 emissions in an experiment involving mid- and end-season drainage and additions of organic material at IRRI in the Philippines.
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 58 (2000), S. 179-199 
    ISSN: 1573-0867
    Schlagwort(e): climate change ; databases ; GIS ; methane ; rice ; soils ; weather
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract As part of a series of papers describing the use of a simulation model to extrapolate experimental measurements of methane (CH4) emissions from rice fields in Asia and to evaluate the large-scale effect of various mitigation strategies, the collation and derivation of the spatial databases used are described. Daily weather data, including solar radiation, minimum and maximum temperatures, and rainfall were collated from 46 weather stations from the five countries in the study, namely China, India, Indonesia, Philippines, and Thailand. Quantitative soil data relevant to the input requirements of the model were derived by combining data from the World Inventory of Soil Emissions (WISE) database, the ISIS database, and the FAO Digital Soil Map of the World (FAO-DSMW). These data included soil pH; organic carbon content; sand, silt, and clay fractions; and iron content for top and subsoil layers, and average values of bulk density and available water capacity for the whole profile. Data on the areas allocated to irrigated, rainfed, upland, and deepwater rice at the province or district level were derived from the Huke & Huke (1997) database developed at IRRI. Using a geographical information system (GIS), a series of georeferenced data sets on climate, soils, and land use were derived for each country, at the province or district level. A summary of the soil-related derived databases is presented and their applicationn for use in global change modeling discussed.
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  • 6
    ISSN: 1573-0867
    Schlagwort(e): rice ; model ; simulation ; carbon dynamics ; China ; India ; Indonesia ; Philippines ; Thailand ; estimates
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The process-based crop/soil model MERES (Methane Emissions from Rice EcoSystems) was used together with daily weather data, spatial soil data, and rice-growing statistics to estimate the annual methane (CH4) emissions from China, India, Indonesia, Philippines, and Thailand under various crop management scenarios. Four crop management scenarios were considered: (a) a 'baseline' scenario assuming no addition of organic amendments or field drainage during the growing season, (b) addition of 3,000 kg DM ha−1 of green manure at the start of the season but no field drainage, (c) no organic amendments but drainage of the field for a 14-d period in the middle of the season and again at the end of the season, and (d) addition of 3,000 kg DM ha−1 of green manure and field drainage in the middle and end of the season. For each scenario, simulations were made at each location for irrigated and rainfed rice ecosystems in the main rice-growing season, and for irrigated rice in the second (or 'dry') season. Overall annual emissions (Tg CH4 yr−1) for a province/district were calculated by multiplying the rates of CH4 emission (kg CH4 ha−1 yr−1) by the area of rice grown in each ecosystem and in each season obtained from the Huke and Huke (1997) database of rice production. Using the baseline scenario, annual CH4 emissions for China, India, Indonesia, Philippines, and Thailand were calculated to be 3.73, 2.14, 1.65, 0.14, and 0.18 Tg CH4 yr−1, respectively. Addition of 3,000 kg DM ha−1 green manure at the start of the season increased emissions by an average of 128% across the five countries, with a range of 74–259%. Drainage of the field in the middle and at the end of the season reduced emissions by an average of 13% across the five countries, with a range of −10% to −39%. The combination of organic amendments and field drainage resulted in an increase in emissions by an average of 86% across the five countries, with a range of 15–176%. The sum of CH4 emissions from these five countries, comprising about 70% of the global rice area, ranged from 6.49 to 17.42 Tg CH4 yr−1, depending on the crop management scenario.
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  • 7
    ISSN: 1573-0867
    Schlagwort(e): methane ; rice ; Oryza sativa ; anaerobic ; model ; simulation ; carbon dynamics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The MERES (Methane Emissions from Rice EcoSystems) simulation model was tested using experimental data from IRRI and Maligaya in the Philippines and from Hangzhou in China. There was good agreement between simulated and observed values of total aboveground biomass, root weight, grain yield, and seasonal methane (CH4) emissions. The importance of the contribution of the rice crop to CH4 emissions was highlighted. Rhizodeposition (root exudation and root death) was predicted to contribute about 380 kg C ha−1 of methanogenic substrate over the season, representing 37% of the total methanogenic substrate from all sources when no organic amendments were added. A further 225 kg C ha−1 (22%) was predicted to come from previous crop residues, giving a total of around 60% originating from the rice crop, with the remaining 41% coming from the humic fraction of the soil organic matter (SOM). Sensitivity analysis suggested that the parameter representing transmissivity to gaseous transfer per unit root length (λr) was important in determining seasonal CH4 emissions. As this transmissivity increased, more O2 was able to diffuse to the rhizosphere, so that CH4 production by methanogens was reduced and more CH4 was oxidized by methanotrophs. These effects outweighed the opposing influence of increased rate of transport of CH4 through the plant, so that the overall effect was to reduce the amount of CH4 emitted over the season. Varying the root-shoot ratio of the crop was predicted to have little effect on seasonal emissions, the increased rates of rhizodeposition being counteracted by the increased rates of O2 diffusion to the rhizosphere. Increasing the length of a midseason drainage period reduced CH4 emissions significantly, but periods longer than 6–7 d also decreased rice yields. Organic amendments with low C/N were predicted to be more beneficial, both in terms of enhancing crop yields and reducing CH4 emissions, even when the same amount of C was applied. This was due to higher rates of immobilization of C into microbial biomass, removing it temporarily as a methanogenic substrate.
    Materialart: Digitale Medien
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  • 8
    ISSN: 1573-5060
    Schlagwort(e): AFLP ; isozyme ; ISSR ; molecular marker ; RAPD ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract We have examined the effectiveness of similar numbers of markers from four molecular marker systems (AFLP, isozymes, ISSR and RAPD) for revealing genetic diversity and discriminating between infraspecific groups of Oryza sativa germplasm. Each marker system classifies the germplasm into three major groups (most effectively with isozymes and AFLPs), but with differences (primarily with ISSR) between the precise classifications generated. However, at the highest levels of genetic similarity there was only partial agreement as to relationships between individual accessions when different markers were used. When variance was partitioned among and within the three subspecific groups, although the differences were not significant, greater variation was found among than within groups using AFLP and isozymes, with the reverse for RAPD and ISSR. Measurement of polymorphism using average heterozygosity and effective number of alleles gave similar results for each marker system. These results are discussed in relation to various genetic resources conservation activities, and the advisability of extrapolating to other sets of germplasm particularly of other crop species.
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 116 (2000), S. 105-110 
    ISSN: 1573-5060
    Schlagwort(e): PCR ; RAPDS ; rice ; salt-tolerance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The genetic diversity of three salinity tolerant rice varieties Pokkali, Nona-Bokra and Bicol was investigated using random amplified polymorphic DNAs(RAPDs). High yielding susceptible variety IR29 was used as check for comparison. The salinity performance of these varieties were tested by using rapid screening techniques at seedling stage. One hundred primers tested of which 42 revealed differences between Pokkali & Nonabokra, 43 between Pokkali & Bicol and 50 between Nonabokra and Bicol. Polymorphism differences between IR29 - Pokkali, IR29 - NonaBokra and IR29 - Bicol were 47%, 53% and 31%, respectively. Four primers amplified specific fragments that appeared in all the three salt tolerant varieties but not in the salt susceptible variety IR29. Primer UBC 9 (5′-CCTGCGCTTA-3′ produced a prominent diagnostic fragment of approximately 1600 bp; primer UBC 244 (5′-CAGCCAACCG-3′) generated a fragment of about 800 bp in the salt-tolerant varieties; primer UBC 251 (5′-CTTGACGGGG-3′) amplified one polymorphic band of 1100 bp and primer UBC 267(5′-CCATCTTGTG-3′) yielded a relatively weak polymorphic band of 1100 bp.
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  • 10
    ISSN: 1573-5036
    Schlagwort(e): mechanical impedance ; Oryza sativa ; rice ; roots ; screening ; wax layers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The development of a wax layer method for screening the ability of rice (Oryza sativa L.) roots to overcome mechanical impedance is described. Wax layers (3 mm thick) made of mixtures of white soft paraffin and paraffin wax were installed 50 mm deep in tubes of sand. The sand was watered with nutrient solution and planted with 3-d old rice seedlings. The numbers of root axes per plant that had penetrated the wax layers 24 d after planting were counted. The ratio of penetrated to total root axes per plant gave a misleading measure of root penetration ability, as rice varieties differed in the ratio of penetrated to total axes in a low impedance (3% wax) control. In non-flooded conditions, a 60% wax layer decreased root penetration (number of roots penetrating the wax layer per plant) to a mean of 74% of the low impedance control, whereas an 80% wax layer decreased mean root penetration to 31% of the control. The best measure of root penetration in non-flooded conditions was the number of axes penetrating an 80% wax layer. Flooding decreased root penetration of a 60% wax layer to a mean of 26% of the low impedance control. The best measure of root penetration in flooded conditions was the number of axes penetrating a 60% wax layer.
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  • 11
    ISSN: 1573-5036
    Schlagwort(e): drought ; Heterodera sacchari ; nematode ; Oryza glaberrima ; Oryza sativa ; plant water status ; rice ; West Africa
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract This study was undertaken to examine the influence of cyst nematodes (Heterodera sacchari) and drought stress, in isolation and combination, on the water status and growth of rice in Côte d'Ivoire. Drought is considered one of the main yield-limiting factors to upland rice in West Africa, while H. sacchari is emerging as a potentially serious pest. A field study conducted during 1997 at the West Africa Rice Development Association (WARDA) showed that under low water availability in sandy soil, damage to a nematode susceptible Oryza sativa (cv IDSA6) is significant. Visual drought score and physiological parameters were significantly affected (P≤0.01) in plots with a mean population density of 39.4 H. sacchari eggs ml-1 soil at 81 days after sowing, compared to plots with a mean density of 1.2 eggs ml-1. Stomatal conductance, leaf water potential and relative water content were lower, leaf chlorophyll content was greater, and near total yield losses were observed in higher nematode density plots. In 1-l pots following 5 days of imposed drought, the H. sacchari-resistant and drought-resistant O. glaberrima (cv CG14) showed a reduction in stomatal conductance of 73% and in leaf water potential by 0.96 MPa (54%). The effect of H. sacchari (473 eggs l-1 soil) inoculated as cysts to soil before sowing was similar but less severe. The combination of both stresses had similar but yet more pronounced effects than the single stresses on stomatal conductance, leaf water potential and leaf dry weight in CG14. IDSA6 responded in a similar manner to CG14, but less acutely to both stresses. Only the combined stresses resulted in a significant (P≤0.05) reduction in osmotic potential and root dry weight compared with the control, for either CG14 or IDSA6. It is suggested that H. sacchari increased the effects of drought and drought-related losses. This may give a false impression of drought susceptibility in field screening for cultivars, and complicate models which predict crop damage caused by nematodes.
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  • 12
    ISSN: 1573-5036
    Schlagwort(e): Cyanobacteria ; `in situ' N2 fixation ; N fertilizer ; 15N balance ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract This study investigate the potential contribution of nitrogen fixation by indigenous cyanobacteria to rice production in the rice fields of Valencia (Spain). N2-fixing cyanobacteria abundance and N2 fixation decreased with increasing amounts of fertilizers. Grain yield increased with increasing amounts of fertilizers up to 70 kg N ha-1. No further increase was observed with 140 kg N ha-1. Soil N was the main source of N for rice, only 8–14% of the total N incorporated by plants derived from 15N fertilizer. Recovery of applied 15N-ammonium sulphate by the soil–plant system was lower than 50%. Losses were attributed to ammonia volatilization, since only 0.3–1% of applied N was lost by denitrification. Recovery of 15N from labeled cyanobacteria by the soil–plant system was higher than that from chemical fertilizers. Cyanobacterial N was available to rice plant even at the tillering stage, 20 days after N application.
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  • 13
    ISSN: 1573-5060
    Schlagwort(e): drought resistance ; QTL mapping ; RFLP markers ; rice ; root traits ; vegetative stage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Root morphology under well-watered conditions sampled on two occasions and under low-moisture stress was studied in a randomly chosen subset of 56 doubled haploid lines derived from a cross between IR64 and Azucena at two growth stages during the vegetative stage. A molecular map of the same population served as the basis for locating QTLs controlling root morphology and associated traits. The region flanking the RFLP markers RZ730 and RZ801 on chromosome 1 were associated with plant height in all three sampling environments. This position corresponds to sd-1 a semi-dwarfing gene. A total of 15 QTL were detected at the two developmental stages, of which only three QTL were common. Region flanked by RG157 and RZ318 (chromosome 2) contained QTL for root thickness under two different developmental stages. In total, 21 QTL for different traits were detected under low-moisture stress condition. While two QTL for plant height on chromosomes 1 and 3 were common, none of the loci for root morphological traits was common between the two different moisture regimes. The chromosomal segment between RG171 and RG157 contained QTL controlling tiller number per plant, total root length, root volume and total root number per plant. Absence of common QTL for root traits between two developmental stages and two moisture regimes suggests the existence of parallel genetic pathways operating at different growth stages and moisture regimes. Root volume and total root number per plant decreased significantly under stress, whereas maximum root length and plant height exhibited non-significant increases under stress.
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  • 14
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 227 (2000), S. 243-248 
    ISSN: 1573-5036
    Schlagwort(e): residual effect of gypsum ; rice ; selenium toxicity ; sulphur ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A field experiment was conducted for 2 years on an alkaline calcareous seleniferous soil to study the effect of different levels of gypsum (0.2 – 3.2 t ha−1) applied to wheat only in the first year on Se accumulation by wheat (Triticum aestivum L.) – rice (Oryza sativa L.) cropping sequence. With gypsum application, grain yield of both rice and wheat crops increased by 0.4 – 0.5 t ha−1; the increase in straw yield was 0.4 – 1.1 t ha−1. Significant reduction in Se accumulation by wheat was observed with gypsum application up to 0.8 t ha−1 and its residual effect was evident on the following crops for 2 years. Reduction in Se accumulation varied from 53 to 64% in wheat grain, 46 to 49% in wheat straw, 35 to 63% in rice grain and 36 to 51% in rice straw with an application of gypsum at 0.8 t ha−1. A corresponding increase in S concentration was observed. In the gypsum-treated plots, the ratio of S:Se increased by 6 – 8 times in wheat and 3 – 6 times in rice. Reduction in Se accumulation by crop plants through gypsum application may help in lowering the risk of Se over-exposure of animals and humans that depend on diet materials grown on high selenium soils.
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  • 15
    ISSN: 1573-5060
    Schlagwort(e): durable resistance ; hybrids ; maize ; marker-assisted selection ; pearl millet ; resistance gene deployment strategies ; rice ; sorghum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Marker-assisted selection (MAS) for resistance genes (R-genes), identified using molecular markers and quantitative trait loci (QTL) analysis, is now possible in many crops. MAS can be used to pyramid several R-genes into a single host genotype. However, this may not provide durable genetic resistance because the pathogen is exposed to a full homozygous pyramid during hybrid seed production and to a full heterozygous pyramid in the resultant hybrid. Alternative gene deployment strategies that generate genetic variability were analysed, for hybrid cereal cultivars of pearl millet, maize, sorghum and rice, using maintainer lines (B-lines) with two smaller complementary pyramids. An F1 seed parent, produced on two such B-lines, can be used to produce a three-way hybrid. All target loci are heterozygous for resistance alleles in the F1 seed parent, and the pathogen is exposed in the hybrid to a host population that is heterogeneous and heterozygous for alleles at the resistance loci targeted by MAS. Alternatively, single-cross hybrids can be made on seed parents that are maintained by two B-lines that differ for the complementary resistance gene pyramids. In a cross-pollinated crop, the B-lines are allowed to intermate to produce a synthetic B-line. In an inbreeding crop, the B-lines are equivalent to a two-component multiline variety. In inbreeding crops, because there is no intermating between the B-line components, the resultant synthetic seed parents have a higher frequency of genotypes with resistance alleles (R-alleles) at several resistance loci. However, in both cross-pollinated and inbreeding crops the genotypic structure in the hybrids is almost the same. All alternatives to a single-cross hybrid having a full pyramid produce hybrid cultivars having lower frequencies of resistance alleles. The frequency of genotypes having R-alleles at several loci increases greatly in both seed parent and hybrid when the overall frequency of R-alleles in the maintainer lines increases. This is simply done by adding a maintainer line that has a full pyramid or by the component lines having overlapping pyramids.
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  • 16
    Digitale Medien
    Digitale Medien
    Springer
    European journal of plant pathology 105 (1999), S. 743-751 
    ISSN: 1573-8469
    Schlagwort(e): rice ; rice bacterial blight ; Xanthomonas oryzae ; pv. oryzae ; virulence analysis ; environmental effects ; phylogeny
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Eight Philippine races of Xanthomonas oryzae pv. oryzae have been identified based on virulence phenotypes observed on a set of five differential varieties. One of these, Race 3, was found to consist of two phylogenetically distinct lineages based on DNA fingerprinting analysis. To determine, if the two lineages could be differentiated based on host-specificity, 186 strains of Race 3 were analyzed with additional fingerprints and 76 selected isolates with additional differential rice varieties. The strains were separated into 36 haplotypes clustering in three groups (IS1113-B, -C, and -G) at the 75% similarity level. Isolates varied in their reaction to a rice line carrying the resistance gene Xa7, however, the variability was not consistent within lineage. Aggressiveness of isolates belonging to lineage IS1113-B and -G was significantly greater when tested during the dry season than when tested during the wet season. However, no such differences were evident for isolates from lineage IS1113-C, indicating that environmental effects presumably light and temperature are genotype-specific.
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  • 17
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 53 (1999), S. 229-235 
    ISSN: 1573-0867
    Schlagwort(e): controlling factor ; diel methane emission pattern ; dissolved methane ; rice ; soil temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Methane emissions from flooded rice grown under greenhouse conditions were monitored using a closed chamber technique. The three rice cultivars showed similar diel emission patterns though the amplitudes differed. Variation in emissions (maximum emission rate) from the different cultivars ranged from 0.164–0.241 mg/pot/h at tillering stage, 0.714–2.334 mg/pot/h at heading stage, 0.399–1.393 mg/pot/h at ripening stage. The methane emissions increased in the morning at accelerating rates, reached a maximum in the early afternoon, then decreased rapidly to constant rates during the night. The diel emission pattern was modeled using a Gaussian equation for daytime, and a constant for nocturnal emissions. Applying an Arrhenius equation, more than 90% of the diel variation of methane emissions could be predicted from soil temperature fluctuations. The predictions improved by using a diffusion model based on soil temperature and dissolved methane concentrations in soil solution. Soil temperature and methane concentration in soil solution are the two major factors controlling diel methane emissions.
    Materialart: Digitale Medien
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  • 18
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 55 (1999), S. 89-94 
    ISSN: 1573-0867
    Schlagwort(e): algae ; flooded soils ; N cycling ; nitrogen ; 15N ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Algal N labelled with 15N added to a flooded soil in laboratory columns without plants was studied to determine the changes over time in the fate of N assimilated by algae and to study how its fate is affected by (a) exclusion of light simulating complete closure of the rice canopy, and (b) addition of fertilizer-NH4 *. In the light but with no added fertilizer-N there was little net mineralization of the added algal N during the first 4 weeks, but after 8 weeks 42% had been mineralized, of which 95% was denitrified. Exclusion of light caused net mineralization to proceed more rapidly in the first 4 weeks due to the death of algal cells and lowered reassimilation. After 8 weeks 51% had been mineralized, of which 54% was denitrified, 16% volatilized and 30% was present as KCl exchangeable NH4 +-N. Application of fertilizer-NH4 + apparently caused mineralization of 25% of the algal N within one week but the results were probably affected by pool substitution in which labelled N mineralized to NH4 +-N was diluted with fertilizer – NH+ 4 and then immobilized leaving more labelled NH4–N in the mineral pool. After 8 weeks, 42% of algal N had been mineralized, of which 69% was estimated to have been denitrified, 19% lost through NH3 volatilization and 12% remained as extracted NH4 ++NO- 3. Uptake of N by a rice crop would reduce the gaseous losses. Algal N was mineralized quickly enough to be available during the growing season of a rice crop and, depending on field conditions, algae may have a role in assimilating N and protecting it from loss as well as being a major driving force for NH3 volatilization through diurnal increases in pH.
    Materialart: Digitale Medien
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  • 19
    ISSN: 1573-0867
    Schlagwort(e): ammonia volatilization ; green manure ; inhibitor ; NBPT ; rice ; urea hydrolysis ; wheat straw
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Using a forced-draft chamber technique, the suppression of NH3 volatilization losses by applying N-(n-butyl) thiophosphoric triamide (NBPT) was studied in an alkaline sandy loam soil amended with green manure or wheat straw. Applied urea was completely hydrolysed in 12, 8 and 6 days in unamended, green manure and wheat straw amended soil, respectively. By applying 0.5% (w/w of urea) NBPT, complete hydrolysis of urea was delayed up to 16 days in the unamended soil, whereas in wheat straw amended soil urea hydrolysis was completed by the 12th day even when it was treated with 2% NBPT. Applied at 1 or 2% level, NBPT delayed the NH3 volatilization to the 4th day after application of urea in green manure or wheat straw amended soil. Hydrolysis of urea was more effectively retarded by applying NBPT in the unamended soil than in soil amended with green manure or wheat straw. In the unamended soil, 7.1% of the applied urea was lost through NH3 volatilization. The losses were reduced to 1.2 and 0.7% by applying 0.5 and 1% NBPT, respectively. Enhanced NH3 volatilization caused by the green manure or wheat straw was counteracted by applying NBPT.
    Materialart: Digitale Medien
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  • 20
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 54 (1999), S. 189-197 
    ISSN: 1573-0867
    Schlagwort(e): boron toxicity ; oilseed rape ; rice ; rotation ; soil boron ; seed yield
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Yields in intensive rice (Oryza sativa L.) -based rotations in Asia are stagnating or declining because of decreasing nutrient availability and depletion of soil nutrient reserves. In the rape (Brassica napus L.)–rice–rice cropping rotation of southeast China, where boron (B) deficiency is widespread and B fertiliser is needed to correct it, our objective was to evaluate the risks of fertiliser-induced B toxicity in oilseed rape and in rice. Response of oilseed rape to B fertiliser application at rates up to 6.6 kg B ha-1 was studied in seven field experiments on three contrasting soils of Zhejiang province, alluvial, red and blue-purple soils. The effects of up to 3.3 kg B ha-1 in 1 year, 6.6 kg B ha-1 in 2 years or 9.9 kg B ha-1 in 3 years were studied on oilseed rape and the one or two rice crops grown immediately after rape in each annual crop rotation. Soils varied in initial hot CaCl2-extractable B in the 0–15-cm layer from 0.24 to 0.99 mg kg-1. At the stem elongation stage of oilseed rape, 3.3 kg B ha-1 depressed shoot dry matter on soils with low clay and organic matter content. However, the subsequent effects of high fertiliser B on seed yield were minimal even at 6.6 kg B ha-1. Moreover, the application of a total of 6.6 or 9.9 kg B ha-1 as successive annual applications of 3.3 kg B ha-1 to oilseed rape generally had no negative effect on seed yield of oilseed rape. The single exception was on a sandy alluvial soil where a total of 6.6 kg B ha-1 in 2 years slightly depressed seed yield of oilseed rape. Grain yields of rice crops grown in rotation after oilseed rape were unaffected by B applications up to 6.6 kg ha-1. The minimal effects of a total of 6.6 kg B ha-1 applied over 2 years on seed yield were consistent with the modest increase in hot CaCl2-extractable B levels. It is concluded that there is limited risk of B toxicity from the use of borax fertiliser at up to 4–8 times recommended rates in rape–rice cropping rotations in southeast China. The low risk of B toxicity can be attributed to the relatively high B removal in harvested seed, grain and stubble, the redistribution of fertiliser B by leaching in the 0–60 cm layer and to B sorption.
    Materialart: Digitale Medien
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  • 21
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 208 (1999), S. 161-166 
    ISSN: 1573-5036
    Schlagwort(e): 14C-urea ; flooded soil ; molecular urea ; 15N-urea ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A pot experiment was conducted with rice to study the relative absorption of urea in molecular form compared to the other forms of N produced in soil from the applied urea. A method involving application of 14C-labelled urea and 15N-labelled urea alternately in two splits was used to quantify the absorption of molecular urea and other forms of N formed from it. Biomass production and N uptake were greater in plants grown under flooded soil conditions than in plants grown under non-flooded (upland) conditions. Absorption of N by rice increased with increasing rate of urea application up to 250 mg pot−1 and declined thereafter. The absorption of urea from the flooded soil constituted 9.4% of total N uptake from applied N compared to only 0.2% from the non-flooded. Under submerged conditions, absorption of urea from topdressing was about twice that from basal application at planting. High water solubility of the fertilizer and better developed rice root system might have enhanced the absorption of molecular urea by flooded rice, especially from topdressing. Thus, in the flooded rice system, the direct absorption of molecular urea from topdressing accounted for 6.3% of the total N uptake from added urea. Under upland condition, it was 0.12%.
    Materialart: Digitale Medien
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  • 22
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 212 (1999), S. 133-141 
    ISSN: 1573-5036
    Schlagwort(e): deficiency ; hydroponic ; iodate ; iodide ; iodine ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract In the Xinjiang province of western China, conventional methods of iodine (I) supplementation (i.e, goiter pills and iodinated salt) used to mitigate I deficiencies were ineffectual. However, the recent addition of KIO3 to irrigation waters has proven effective. This study was conducted to determine the effects of I form and concentration on rice (Oryza sativa L.) growth, I partitioning within the plant, and ultimately to assist in establishing guidelines for incorporating I into the human food chain. We compared IO3 − vs. I− in order to determine how these chemical species differ in their biological effects. Rice was grown in 48 L aerated tubs containing nutrient solution and IO3 − or I− at 0, 1, 10, or 100 μM concentrations (approximately 0, 0.1, 1, and 10 mg kg−1 I). The IO3 − at 1 and 10 μM had no effect on biomass yields, and the 100 μM treatment had a small negative effect. The I− at 10 and 100 μM was detrimental to biomass yields. The IO3 − treatments had more I partitioning to the roots (56%) on average than did the I− treatments (36%), suggesting differences in uptake or translocation between I forms. The data support the theory that IO3 − is electrochemically or biologically reduced to I− prior to plant uptake. None of the treatments provided sufficient I in the seed to meet human dietary requirements. The I concentration found in straw at 100 μM IO3 − was several times greater than seed, and could provide an indirect source of dietary I via livestock feeding on the straw.
    Materialart: Digitale Medien
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  • 23
    ISSN: 1573-5087
    Schlagwort(e): 2,6-diisopropylphenoxyacetic acid ; gibberellin ; Oryza sativa ; rice ; shoot elongation ; uniconazole
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract 2,6-Diisopropylphenoxyacetic acid (DIPA), a promoter of growth and flowering of Sagittaria species, was found to improve the shoot growth of rice plants treated with uniconazole, an inhibitor of gibberellin (GA) biosynthesis. In a modified micro-drop bioassay using semi-dwarf rice, Oryza sativa L. cv. Tan-ginbozu, in which uniconazole had been incorporated into the agar medium, a significant recovery from growth inhibition was observed for both the 3rd and the 4th leaf sheaths but not for the 2nd sheath. In greenhouse experiments, uniconazole-treated rice plants partially recovered from growth inhibition when DIPA was applied after uniconazole treatment, whereas DIPA applied with, or before, uniconazole treatment did not improve growth. The levels of GA1 and GA20 in the rice plants treated with uniconazole plus DIPA were almost equal to those of the untreated controls, indicating that the observed growth recovery is attributable to the restoration of GA biosynthesis by DIPA.
    Materialart: Digitale Medien
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  • 24
    Digitale Medien
    Digitale Medien
    Springer
    Plant growth regulation 28 (1999), S. 29-39 
    ISSN: 1573-5087
    Schlagwort(e): anther ; sugars ; ethylene inhibitors ; correlative signal ; rice ; spikelet
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Rice (Oryza sativa L. cv. Lalat) was grown in pots under open field conditions during the wet season of 1997. Attempts were made to manipulate the growth and development of the male gametophyte, located on the basal region of the panicle, by exogenous application of chemicals regulating formation/action of ethylene and compare grain setting in the spikelets bearing few grain. Application of ethylene action (AgNO3) and synthesis inhibitor (Co(NO3)2; paclobutrazol and uniconazole) improved grain setting in the spikelets and the ethylene releasing substance 2-chloroethyle phosphonic acid (CEPA) depressed it compared to the control. The ethylene inhibitors promoted dry mass accumulation and concentrations of starch and reducing sugars in the anthers of the basal spikelets, while CEPA reduced the level of these carbohydrates significantly. The ethylene inhibitors helped in the survival of more numbers of pollen in these anthers, but CEPA depressed their number significantly. Promotion of growth of the basal anthers was accompanied by a concomitant reduction in the concentration of nonreducing sugars and enhanced activities of acid invertase and sucrose synthase enzymes. It is concluded that male gametophyte development of the basal spikelets of rice is susceptible to ethylene at the stage of pollen mitosis. The possibility of the hormone interfering in carbohydrate metabolism of the anther during this stage of development is discussed.
    Materialart: Digitale Medien
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  • 25
    ISSN: 1573-5060
    Schlagwort(e): microsatellite ; Oryza sativa ; polymorphism ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Ten microsatellite loci were analysed for 43 cultivars or breeding lines of rice. Polymorphism-Information-Content values ranged from 0.62 to 0.92. The microsatellite markers were found to be useful for cultivar identification and assessment of genetic relationships. Most of the cultivars could be uniquely identified by at least one microsatellite marker. Genetic heterogeneity was detected within rice samples by amplification of microsatellites from DNA extracted from multiple individual plants and also from bulked DNA preparations.
    Materialart: Digitale Medien
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  • 26
    ISSN: 1573-5087
    Schlagwort(e): 1-aminocyclopropane-1-carboxylate (ACC) oxidase ; ethylene ; Oryza sativa ; ozone ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Exposure to ozone at 1 µl l−1 for 6 h induced ethylene production in rice (Oryza sativa L. cv. Hitomebore) leaves. The stimulation of ethylene production was detectable 2 h after the start of the exposure to ozone, and lasted for 6 h after the exposure. A 429-bp cDNA fragment encoding ACC oxidase was obtained by RT-PCR from ozone-treated rice leaves. Its nucleotide sequence and deduced amino-acid sequence had 97.2% and 94.4% identity, respectively, to those of OS1A1COX, which was previously obtained from deepwater rice. The abundance of the cDNA increased in accordance with the induction of ethylene production by the exposure to ozone.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 27
    ISSN: 1573-5060
    Schlagwort(e): rice ; Oryza sativa L. ; photoperiod sensitive genic male sterility (PGMS) ; protoplasts ; flow cytometry ; tetraploid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Plants were regenerated from protoplasts isolated from embryonic suspension cultures of N5047S, a photoperiod sensitive genic male sterile (PGMS) Japonica rice line. Flow cytometric analyses of nuclear DNA content identified some tetraploid regenerates whose agronomic traits could be distinguished from diploid regenerates. Pollen and female fertility of diploid protoplast-derived clones grown under different light and temperature conditions was compared. A promising PGMS protoplast clone, ZAU11S, was developed from these clones. Its male sterility was confirmed as a photoperiod × temperature interaction type.
    Materialart: Digitale Medien
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  • 28
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 216 (1999), S. 117-127 
    ISSN: 1573-5036
    Schlagwort(e): image analysis ; NIH Image ; rice ; root length
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Algorithms for estimating root length by image analysis should lead to results that have no systematic error (bias), be insensitive to preferential root orientation, valid across a wide range of sample sizes and adjust for overlap between roots in samples, to reduce the effort needed in spreading out root systems. We propose a new algorithm that forms a compromise between small bias and robustness (insensitivity to variation in sample size and preferential root orientation), and provide a simple way of dealing with root overlap. Image analysis was performed on a Macintosh computer using the public domain NIH Image program. The digital image of the root was processed to get the thinned image (skeleton). The numbers of orthogonally and diagonally connected pairs of pixels (N o and N d, respectively) in the skeleton were counted separately and used for length (L) calculation. A new length calculation equation was introduced so that the effect of orientation on length calculation was minimized; L=[N d 2+(N d+N o/2)2]1/2+N o/2. The maximum error due to orientation of a single line was evaluated for an ideal line, and the analysis revealed that the new equation was less affected by orientation than previous equations. Copper wire and rice (Oryza sativa L.) roots containing both primary and fine secondary root were measured manually and with image analysis. The two methods showed good agreement within 1.5%. The proposed image analysis method yielded length estimates with CV from 0.23 to 0.88%, which was lower than the CVs of the line-intersect method. Moreover, the lengths of overlapping samples were calculated correctly because the image analysis method distinguished an overlapping pixel from a thinned image, while the calculation with the line-intersect method showed underestimation because overlaps were not considered in that method.
    Materialart: Digitale Medien
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  • 29
    ISSN: 1573-5060
    Schlagwort(e): Bph-1 ; linkage analysis ; mapping ; RAPD ; RFLP ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract We report the tagging of a brown planthopper (BPH) resistance gene (Bph–1) in rice using RAPD and RFLP markers. The Korean rice variety ‘Gayabyeo’ has dominant duplicate genes including Bph–1 conferring resistance to biotype 1 of BPH. Bulked segregant RAPD analysis was employed for rapid identification of DNA markers linked to resistance genes. For tagging these two genes, an F2F3 population from a ‘Gayabyeo’ × ‘Nagdongbyeo’ cross was developed and evaluated for BPH resistance. Three bulked DNAs from two groups of homozygous BPH resistant (each for Bph–1 and the other unknown gene) and homozygous susceptible F2 plants were analyzed by RAPD using 140 random oligomers. One primer, OPD–7 yielded a 700-bp fragment that was present in Gayabyeo and resistant F2 plants (homozygous for Bph-1 locus) but absent in Nagdongbyeo and susceptible F2 plants. Cosegregation of this marker with Bph-1 was verified using an F2 population segregating for Bph-1. Chromosomal regions surrounding the Bph-1 were examined with additional RFLP and microsatellite markers on chromosome 12 to define the location of the RAPD marker and Bph-1. Use of this RAPD marker could facilitate early selection of resistant lines for BPH.
    Materialart: Digitale Medien
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  • 30
    ISSN: 1573-5060
    Schlagwort(e): AFLP ; DNA fingerprinting ; isozymes ; RAPD ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A survey of the genetic diversity among the major cuban rice cultivars was conducted using isozyme, RAPD and AFLP markers. Polymorphisms were detected for esterases, peroxidases, alcohol dehydrogenases and polyphenoloxidases systems; 21 RAPD primers and four AFLP primer combinations. Heterozygosity arithmetic mean value (Hav(p)), the effective multiplex ratio (EMR) and the marker index (MI), were calculated for isozyme, RAPD and AFLP markers. The mean value of genetic similarity among the different varieties was 0.92 for isozyme, 0.73 for RAPD and 0.58 for AFLP analyses. Thus, AFLP were able to detect polymorphisms with higher efficiency than RAPD (+15%) and isozyme (+34%). Data from the isozyme, RAPD and AFLP analyses were used to compute matrices of genetic similarities. The efficiency of the UPGMA for the estimation of genetic relatedness among varieties was supported by cophenetic correlation coefficients. The resulting values indicated that the distortion level for the estimated similarities was minimal. The correlation coefficients obtained by the Mantel matrix correspondence test, which was used to compare the cophenetic matrices for the different markers, showed that estimated values of genetic relationship given for isozyme and RAPD markers (r = 0.89), as well as for AFLP and RAPD markers (r = 0.82) were properly related. However, AFLP and isozyme data showed only moderate correlation (r = 0.63). Although the genetic variability found among the different cultivars was low, both RAPD and AFLP markers proved to be efficient tools in assessing the genetic diversity of rice genotypes.
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  • 31
    ISSN: 1573-5060
    Schlagwort(e): doubled haploids ; genetics ; haploids ; induced variation ; Oryza sativa ; ovary culture ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Plants derived from unpollinated ovary culture of ten rice genotypes showed significant variability in agro-morphological characteristics. The ovary-derived plant (H1) populations were completely haploid, doubled haploid or haploid-doubled haploid mixture. Haploids had very drastic reduction in plant height, panicle length, grain length, breadth and number and spikelet fertility (0.0%–2.1%). Doubled haploids from the hybrid of UPRI 95–121 × UPRI 95–165 were normal with fertility ranging between 69.6% and 97.7%. A genetic segregation in ratio of 1:1 was observed for five pigmentation characters in the H1 population derived from hybrid UPRI 95–122 × UPRI 95–165. Plant height showed the largest coefficient of variability (28.5%) followed by the number of spikelets per panicle (24.2%), number of grains per panicle (22.0%), percent seed set (9.2%) and panicle length (9.0%). The range of variation in the H1 population from fully fertile hybrid PMS 2A (CMS) × IR 31802 (restorer) was similar to its corresponding F2 population for plant height, spikelet fertility and number of grains/panicle. A single clone of plants from the cultivar BG 1321 exhibited complete male sterility but normal female fertility when pollinated with other varieties. Ovary-derived plants from the CMS lines PMS 2A and IR 58025A showed stable male sterility and those from thermosensitive genetic male sterile line UPRI 95–140 showed thermosensitive genetic male sterility. These lines have potential in the hybrid breeding program and are being currently exploited.
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  • 32
    Digitale Medien
    Digitale Medien
    Springer
    Genetic resources and crop evolution 46 (1999), S. 587-598 
    ISSN: 1573-5109
    Schlagwort(e): diversity ; genetic resources ; GIS ; Oryza sativa ; RAPD ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A diverse set of 115 rice varieties from Bangladesh was surveyed using 35 polymorphic RAPD (randomly amplified polymorphic DNA) markers and the genetic structure of this germplasm, encompassing the principal rice ecotypes of Bangladesh (aus, aman and boro), was determined using multivariate analysis. The level of genetic diversity was evaluated and compared with the levels of diversity found within other rice growing areas of the world. Geographical information systems analysis using Atlas-GIS was employed to analyse and present the geographic distribution of genetic diversity across Bangladesh, and cluster analysis was used to test the efficiency of selection of material for a core collection.
    Materialart: Digitale Medien
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  • 33
    Digitale Medien
    Digitale Medien
    Springer
    Molecular breeding 5 (1999), S. 319-327 
    ISSN: 1572-9788
    Schlagwort(e): Cybrid ; cytoplasmic male sterility ; mitochondrial DNA ; protoplast fusion ; rice ; wild abortive cytoplasm
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Wild abortive cytoplasmic male sterility has been extensively used in hybrid seed production in the tropics. Using protoplast fusion between cytoplasmic male sterile and fertile maintainer lines; we report here, transfer of wild abortive cytoplasmic male sterility to the nuclear background of RCPL1-2C, an advance breeding line which also served as maintainer of this cytoplasm. In total, 27 putative cybrids between V20A and RCPL1-2C and 23 lines between V20A and V20B were recovered and all of them were sterile. DNA blots prepared from the mitochondrial DNA of the cybrid lines from both the sets were probed with orf155 that is known to exhibit polymorphism between the mitochondrial DNA of the male-sterile and fertile maintainer lines. Hybridization of orf155 to 1.3 kb HindIII-digested mitochondrial DNA fragment of the cybrids showed transfer of mitochondrial DNA from wild abortive cytoplasmic male-sterile line to the maintainers, viz. RCPL 1-2C and V20B. Expression of male sterility was confirmed by the presence of sterile pollen grains and the lack of seed setting due to selfing in all the cybrid lines. These cybrids, on crossing with respective fertile maintainers set seeds that in turn, produced sterile BC1 plants. DNA blots from HindIII-digested mitochondrial DNA of these BC1 plants when probed with orf155 again exhibited localization of orf155 in wild abortive cytoplasm-specific 1.3 kb HindIII-digested mitochondrial DNA fragments. This demonstrated that the cytoplasmic male sterility transferred through protoplast fusion retained intact female fertility and was inherited and expressed in BC1 plants. Fusion-derived CMS lines, on pollination with pollen grains from restorer, showed restoration of fertility in all the lines. The results demonstrate that protoplasts fusion can be used for transferring maternally inherited traits like cytoplasmic male sterility to the desired nuclear background which can, in turn, be used in hybrid seed production programme of rice in the tropical world.
    Materialart: Digitale Medien
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  • 34
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 51 (1998), S. 269-279 
    ISSN: 1573-0867
    Schlagwort(e): denitrification ; gas entrapment ; nitrification inhibitors ; 15N ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Denitrification rates (d) in a flooded alkaline clay were measured following addition of either $$^{15} NO_3^ - or^{15} NH_4^ + $$ to the floodwater, by collecting evolved N2 + N2O in an enclosure in the absence or presence of rice plants. Similar estimates of d were obtained in the $$^{15} NO_3^ - $$ treatment when the isotopic composition of the enclosed atmosphere was determined using arc redistribution or direct mass spectrometric analysis. Approximately 90% of the gaseous products of denitrification were physically trapped in the soil five days after $$^{15} NO_3^ - $$ addition. Mechanical shaking of the soil-water system was an effective method for releasing entrapped gas. Denitrification showed a marked diurnal variation in both $$^{15} NO_3^ - $$ and $$^{15} NH_4^ + $$ treatments planted to rice, with higher rates during the day than at night. Measured rates of denitrification were higher in planted than in unplanted pots for both $$^{15} NO_3^ - $$ and $$^{15} NH_4^ + $$ treatments for normal gas sampling. However, evidence was obtained that this was not a real effect, but was due to release of entrapped gas. Denitrification losses corrected for gas entrapment were estimated at 〈5% of applied $$^{15} NH_4^ + $$ . The 15N mass balance indicated that a much larger amount of applied ammonium (15–25%) was lost by NH3 volatilisation. The rate of denitrification corrected for gas entrapment was similar to the rate of nitrification estimated by inhibition of ammonium oxidation. Although the inhibitors 2-ethynylpyridine and acetylene prevented denitrification by effectively inhibiting nitrification of $$^{15} NH_4^ + $$ , the total recovery of 15N in the soil-plant system did not increase. The total recovery of $$^{15} NH_4^ + $$ was 7–9% higher in the presence than in the absence of rice.
    Materialart: Digitale Medien
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  • 35
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 51 (1998), S. 259-267 
    ISSN: 1573-0867
    Schlagwort(e): nitrification ; nitrification inhibitors ; 15N-dilution ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Nitrification rates (n) in the floodwater of an alkaline clay were measured in the absence or presence of rice plants by inhibition of ammonium oxidation and 15N-dilution techniques. Floodwater nitrate concentrations in control treatments showed a marked diurnal variation, and were higher than in the inhibitor treatments after the first day. Ammonium concentrations in floodwater declined exponentially in all treatments, being markedly affected by diffusion and NH3 volatilization but little affected by nitrification and plant uptake. Nitrification rates in floodwater estimated by 15N-dilution were generally higher than the rates estimated by the inhibitor method. Estimates of n were generally higher during daylight hours than at night, and did not differ significantly between planted and unplanted pots. Microbial immobilisation of labelled ammonium and gross N immobilisation were not affected by addition of the nitrification inhibitor 2-ethynylpyridine.
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  • 36
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 53 (1998), S. 71-81 
    ISSN: 1573-0867
    Schlagwort(e): fallow ; legumes ; nitrogen fixation ; rice ; weeds ; West Africa
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Traditional upland rice-based cropping systems in West Africa rely on periods of fallow to restore soil fertility and prevent the build-up of insect pests and weeds. Demographic growth and increased demand for land is forcing many farmers to intensify their rice production systems. Declining fallow length and increasing number of crops before leaving the land to extended fallow result in a significant yield reduction. Promising cropping system alternatives include the use of site specific, weed-suppressing, multi-purpose cover legumes as short duration fallows. Constraints to rice production related to intensification were determined in 209 farmers' fields in four agro-ecological zones during 1994 and 1995. Nitrogen accumulation and weed suppression were evaluated in 54 legume accessions, grown for six months during the dry season, under a range of hydrological and soil conditions in 1994/95. Their effect on the yield of upland rice was determined in 1995. To increase benefits from improved fallow technology, the timing of legume establishment in relation to rice and the effect on crop and weed growth of removing, burning, mulching, or incorporating fallow residues prior to the rice crop were determined. Intensified land use resulted in a significant plot-level yield reduction that was highest in the derived savanna and the bimodal forest zones where it was associated with a doubling of the weed biomass in rice and a significant reduction in soil N supply. Legume fallows appear to offer the potential to sustain rice yields under intensified cropping. Legume biomass was in most instances significantly greater than in the weedy fallow control and several legume species suppressed weed growth. Nitrogen accumulation by legumes varied between 1–200 kg N ha-1 with 30–90% Ndfa. Rice grain yield following legume fallows increased by an average of 0.2 mg ha-1 or 29% above the weedy fallow control. Relay establishment substantially increased legume biomass. However, seeding of the legume at 28 days or earlier significantly reduced grain yield due to interspecific competition. Incorporating or mulching of fallow residues provided no significant yield advantage as compared to burning. Absolute effects varied as a function of site, legume species, and management practice.
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  • 37
    ISSN: 1573-5087
    Schlagwort(e): chilling tolerance ; fatty acids ; galactolipids ; phospholipids ; rice ; somaclonal variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The relationship between chilling tolerance of six rice cultivars – Facagro 57, Facagro 76, Fujisaka 5, Kirundo 3, Kirundo 9 and IR64 -and the fatty acid composition in total lipids, phospholipids, galactolipids and neutral lipids from leaves was studied. Higher double bond index and proportions of linolenic acid in the phospholipid and galactolipid classes were related to cultivar chilling tolerance, but this was not so for the total lipids nor the neutral lipid class. The somaclonal families derived from Facagro 76, Kirundo 3 and Kirundo 9 that showed enhanced chilling tolerance as compared to their original parental cultivar were analyzed for fatty acid composition in phospholipids and galactolipids from leaves. Altered proportions in fatty acid composition in phospholipids, galactolipids or both were found in the somaclonal families derived from Facagro 76 and Kirundo 9, but not from Kirundo 3. These changes most usually resulted in higher double bond index and higher proportions in linoleic and linolenic acids which were related either to lower ratio of C16 to C18 fatty acids or to higher unsaturation in the C18 fatty acid fraction. Different mechanisms thus seem to be implicated in the altered fatty acid composition of somaclones, which may be related to the chilling tolerance improvement of some somaclonal families.
    Materialart: Digitale Medien
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  • 38
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 200 (1998), S. 169-173 
    ISSN: 1573-5036
    Schlagwort(e): ammonium ; copper ; leaf senescence ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The possibility that ammonium (NH 4 + ) accumulation is linked to the senescence of detached rice (Oryza sativa) leaves induced by copper (Cu) was investigated. CuSO4 was effective in promoting senescence of detached rice leaves. Both CuSO4 and CuCl2 induced NH 4 + accumulation in detached rice leaves, indicating that NH 4 + accumulation is induced by copper. Sulfate salts of Mg, Mn, Zn, and Fe were ineffective in inducing NH 4 + accumulation in detached rice leaves. The senescence of detached rice leaves induced by Cu was found to be prior to NH 4 + accumulation. Free radical scavengers, such as glutathione and thiourea, inhibited senescence caused by Cu and at the same time inhibited Cu-induced NH 4 + accumulation. The current results suggest that NH 4 + accumulation is not associated with senescence induced by Cu, but is part of the overall expression of oxidative damage caused by an excess of Cu. Evidence was presented to show that copper-induced ammonium accumulation in detached rice leaves is attributed to a decrease in glutamine synthetase activity and an increase in reduction of nitrate.
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  • 39
    ISSN: 1573-5036
    Schlagwort(e): agar ; agarose ; gels ; mechanical impedance ; Oryza sativa ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Agar and agarose gels were evaluated as systems to mechanically impede roots of rice (Oryza sativa L.). Two-layer gels were used so that seedlings established in a layer of weak gel (0.35% weight/volume) and then grew downwards to encounter a treatment gel of up to 5.0% (w/v). Agarose gels were stronger than agar gels of the same concentration, reaching a maximum penetrometer resistance of 1.2 MPa at a concentration of 5.0%, compared to 0.3 MPa with agar. The 5.0% agar gel stimulated elongation of the seminal axis by 40% in seedlings of variety TN1 (compared with elongation in the 0.2% gel), but decreased it by 15% in the variety Lac 23. Although increasing agarose concentration decreased seminal axis elongation in both varieties, the seminal axis did not reach the lower layer of treatment gel when the concentration of the treatment gel was greater than 2.0%. The decreased root elongation was therefore a non-mechanical inhibition. In experiments conducted using a different batch of agarose, these inhibitory effects were not seen and strong agarose gels stimulated seminal axis elongation. It was concluded that the agar and agarose gel systems studied were unsuitable for studying the effect of mechanical impedance on the elongation of rice roots and that great care should be taken in interpreting the results of experiments using gels as a growth medium.
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  • 40
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 203 (1998), S. 269-277 
    ISSN: 1573-5036
    Schlagwort(e): fertilization ; pH ; phosphorus ; rice ; sodicity ; sodium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Rice seedlings transplanted into sodic soil are exposed to an excess of potentially toxic ions as well as nutritional imbalance, both of which adversely affect their growth and yield. The present study was aimed to investigate the beneficial effects of fertilization with phosphorus and potassium on the plants at varying sodicity levels and also the response of genotypes with known variability in their tolerance to sodicity. In pot-house experiments during two seasons, the alleviating effects of P and K fertilization on three rice genotypes were examined at four sodicity levels. Seedlings of CSR13 and Jaya (both moderately tolerant to sodicity), died by 25–35 days after transplanting in sodic soils of pH 9.7–9.9 where Olsen's P was 12.5 and 14.8 kg/ha, respectively. However, there was no problem of survival or growth in these soils when Olsen's P was 17.6 and 20.8 kg/ha. Depletion in P from 12.0 kg to 10 kg resulted in some mortality of the seedlings even at pH 9.1. Sodicity tolerant genotype CSR10, did show some survival and growth even at pH 9.9 with Olsen's P at 14.8 kg/ha (without P fertilization) which suggests that differences in tolerance to sodicity which exist at genotypic level are not masked by low P. None of the three genotypes showed any survival problem at pH 8.0 and 8.1 with Olsen's P at 8.5 and 8.7 kg/ha, respectively. Seedlings in P fertilized sodic soils not only produced significantly more new roots but also higher root biomass than those in unfertilized sodic soils and these roots seem to have some control on Na uptake as reflected by low Na concentration in the shoots. Thus, P fertilization not only improved P and K status of plants but also reduced the concentration of potentially toxic Na ions in shoots, resulting in better survival, growth and yield. Although fertilization with K alone did improve shoot K content, it had no significant effect on reducing Na. So the mortality of the seedlings or grain yield in K fertilized sodic soils was as good as in control and this could be explained on the basis of lack of any significant difference in Na concentrations in shoots between these two treatments.
    Materialart: Digitale Medien
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  • 41
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 101 (1998), S. 151-156 
    ISSN: 1573-5060
    Schlagwort(e): Oryza sativa L. ; rice ; pure lines ; hybrids ; rough rice yield ; head rice ; total milled rice ; selection index ; path analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract High rough rice yields are important for the profitable production of rice (Oryza sativa L.). The value of rough rice is determined primarily by head rice (whole milled grains) and broken rice, together referred to as total milled rice. The objective of this study was to optimize selection for rough rice yield, head rice, and total milled rice using path-coefficient analysis and selection indices. Thirty-seven long grain pure lines were grown in an experiment at three Arkansas locations during 1993 and 1994. In another test, twenty-eight F1 hybrids obtained from an eight-parent half diallel of long-grain rices were grown at two locations in Arkansas during 1991. For the pure lines, path analysis revealed that rough rice yield was approximately twice as important in determining total milled rice/ha than head rice. Path analysis for the hybrids revealed that rough rice yield was an even greater component in determining total milled rice/ha. Based on yield components for hybrid rice, panicle density had the largest direct effect determining total milled rice/ha. Filled grain/panicle, grain weight, and head rice, in that order, were secondary, but positive, factors determining total milled rice/ha. A selection index for pure lines based only on selection for head rice was 19.2 percent as effective as selection for total milled rice/ha directly. When selection for total milled rice/ha was based solely on rough rice yield, selection was 82.7 percent as effective as selecting for total milled rice/ha directly. Selection to improve total milled rice/ha should concentrate on increasing both rough rice yield and head rice. Rough rice yield should receive greater priority than head rice in selection strategies.
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  • 42
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 102 (1998), S. 151-161 
    ISSN: 1573-5060
    Schlagwort(e): Oryza sativa ; head rice ; seeding date ; rice ; rough rice yield ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The rice (Oryza sativa L.) crop in Arkansas is seeded from late March through early June each year. Farmers need new rice cultivars that not only produce both high rough rice yields and high head rice across this range of seeding dates but do so consistently. Thus, a seeding date study was conducted during 1994 and 1995 at Stuttgart, Arkansas, to study seeding date effects on rough rice yield and head rice and selection for stability. Fourteen rice cultivars were seeded at five dates ranging from late March through mid-June. Kang's yield-stability statistic was used to select cultivars for both high rough rice yields and high head rice as well as stability for both traits. Plant stands from March seedings of each year were significantly lower than for the later seeding dates. Maturity (days to 50 percent heading) was extended at the early seeding dates. Some rice cultivars, such as ‘LaGrue’, had lower and more variable head rice when seeded early. In the June seeding dates each year, ‘Kaybonnet’ produced rough rice yields that were more consistent with yields when planted at the earlier planting dates. ‘Bengal’, ‘Cypress’, ‘Kaybonnet’, and ‘Newbonnet’ were cultivars selected by Kang's stability statistic, which was targeted to select cultivars with stable, high rough rice yields and stable, high head rice. The validity of using Kang's yield-stability statistic for cultivar selection is also evident empirically by the adaptation and wide use of these four cultivars by southern U.S. rice producers. These results indicate that seeding date studies and stability analyses would be useful tools for rice breeders to identify cultivars that will be readily adapted and grown by rice producers.
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  • 43
    ISSN: 1573-5060
    Schlagwort(e): unpollinated ovary culture ; callus induction ; plant regeneration ; chilling pretreatment ; development stage ; medium composition ; rice ; Oryza sativa
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A simple and efficient technique for in vitro unpollinated ovary culture in rice which is also applicable for indica genotypes was developed for breeding and genetic studies. Sampling explants at the auricle distance of 7–12 cm between the two uppermost leaves of a tiller, providing a chilling pretreatment and ovaries with 1/3 of the hulls intact gave optimum response to culture. For callus induction with the spontaneous breaking of ovaries, N6 media supplemented with NAA (2 mg/l) and DMSO (0.6–0.8%) gave a mean PCI value of 3.8% and range of 0.8–12.5% among genotypes. Media combining 2,4,5-T or 2,4-D with NAA in N6 medium also has reasonably good callus induction. For calli induced inside, 2,4-D (0.2–0.5 mg/l), NAA (2 mg/l) and KT (1 mg/l) contained media were superior. The maximum green plant regeneration (PPR) of 77.3% was found with the medium containing NAA 0.25 mg/l, IAA 0.5 mg/l and KT 2.0 mg/l. Significant genotype, medium and their interaction effects for per cent ovary survival and callus induction were observed.
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  • 44
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 99 (1998), S. 155-165 
    ISSN: 1573-5060
    Schlagwort(e): heterosis ; heterosis × environment interaction ; Oryza sativa ; rice ; standard heterosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Moderate to high heterosis for yield and ten related characters was studied in 45 crosses involving six indica (I) and four tropical japonica (J) varieties of rice under three (E1-optimum sowing and high fertility, E2-both optimum sowing and fertility and E3- late sowing and high fertility) environments. Trends of magnitude of heterosis for grain yield and plant height were I/J 〉 I/I 〉 J/J and for days to 50% flowering I/J 〉 J/J 〉 I/I hybrids. Estimates of standard heterosis (%) for grain yield were –64.5 to 146.1 in E1, –70.4 to 82.2 in E2 and –67.2 to 63.8 in E3. Environment E1 seemed to be more favorable for higher heterosis expression than others. Higher heterosis in yield also accompanied heterosis in panicle number, dry matter and spikelet and grain number per panicle. Most estimates for days to flower were negative. Heterotic I/J hybrids also recorded maximum heterosis for earliness. Moderate to low standard heterosis for plant height across environments (2.0 to 13.7%) was recorded. Hybrids were identified in specific environments for direct exploitation in hybrid breeding. Hybrids B4116 / Sarjoo 52, B4122 / Pant Dhan 4 and B4122 / Narendera 359 were more stable than others over three environments.
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  • 45
    Digitale Medien
    Digitale Medien
    Springer
    Genetic resources and crop evolution 45 (1998), S. 399-406 
    ISSN: 1573-5109
    Schlagwort(e): internal transcribed spacer sequences ; nuclear ribosomal RNA genes ; molecular phylogeny ; Potamophila parviflora ; Oryza sativa ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Potamophila parviflora is a wild relative of rice from Eastern Australia. The species was found in the Richmond, Clarence, Macleay and Hastings River systems of northern New South Wales. Existing populations had wider leaves (10–15 mM) than those previously reported (4–6 mM). This species and North American wild rice, Zizania palustris were investigated as genetic resource for rice improvement. Both species, Potamophila , and Zizania , occur in colder climates than rice (Oryza sativa). Sequencing of the ITS1 of the ribosomal genes of these two species indicated that Zizania and Potamophila were more closely related to each other than either was to Oryza sativa. Potamophila has a similar number of chromosomes (n=12) and a greater sequence homology to rice and may thus be a better source of useful genes for rice improvement.
    Materialart: Digitale Medien
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  • 46
    ISSN: 1572-9788
    Schlagwort(e): rice ; anther culturability ; doubled haploid population ; QTLs
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Anther culturability of rice is significantly different between indica and japonica varieties. A doubled haploid (DH) population was established via anther culture of an indica/japonica hybrid on SK3 medium, which had been shown particularly suitable for anther culture of indica/japonica hybrids. For analyzing the quantitative trait loci (QTLs) responsible for anther culturability, anthers of the DH lines were again cultured with SK3 medium and parameters for four traits representing the anther culturability were surveyed and analyzed with the molecular map constructed from the same DH population. The parameters for four major traits were as follows: callus induction frequency (CI), green plantlet differentiation frequency (GPD), albino plantlet differentiation frequency (APD), and green plantlet yield frequency (GPY). All four traits displayed continuous distributions among the DH lines. The correlation coefficients between these traits were also tested and showed that there was no relationship between callus induction and green plantlet differentiation frequencies, but both showed strong positive correlation with the frequency of green plantlet yield. For callus induction frequency, five QTLs were identified on chromosomes 6, 7, 8, 10 and 12. Two QTLs for green plantlet differentiation frequency were located on chromosomes 1 and 9. There was a major QTL for albino plantlet differentiation frequency on chromosome 9. No independent QTL was found for green plantlet yield frequency. The results may be useful in the selection of parents with high response to anther culture for rice haploid breeding and in the establishment of permanent DH populations for molecular mapping.
    Materialart: Digitale Medien
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  • 47
    Digitale Medien
    Digitale Medien
    Springer
    European journal of plant pathology 103 (1997), S. 363-371 
    ISSN: 1573-8469
    Schlagwort(e): blast ; rice ; Magnaporthe grisea ; Pyricularia ; virulence ; genetic variability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The genetic variability among 41 isolates of the blast pathogen (Magnaporthe grisea) from five European rice growing countries was studied. The genealogy of the isolates was investigated by DNA fingerprinting and the results compared to the degree of similarity for (a)virulence factors. Fingerprinting grouped the isolates into five discrete lineages, that typically showed less than 65% band similarity. Within each lineage, two or more haplotypes were detected with a band similarity of 80% or higher. Each lineage showed a characteristic virulence pattern. All isolates of lineage ‘E5’ belonged to the same pathotype. The other lineages were composed of clusters of closely related pathotypes that showed variation for virulence to cultivars with certain known resistance genes, while remaining invariably (a)virulent to others. In most cases, lineage classification of an isolate could be easily inferred by its pathotype. Certain resistance genes and certain lineage-excluding resistance gene combinations appear to provide protection against all of the virulence factors sampled.
    Materialart: Digitale Medien
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  • 48
    ISSN: 1573-5036
    Schlagwort(e): Azorhizobium caulinodans ; crack entry ; intercellular colonization ; naringenin ; rice ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Recently, evidence has been obtained that naturally occurring rhizobia, isolated from the nodules of non-legume Parasponia species and from some tropical legumes, are able to enter the roots of rice, wheat and maize at emerging lateral roots by crack entry. We have now investigated whether Azorhizobium caulinodans strain ORS571, which induces root and stem nodules on the tropical legume Sesbania rostrata as a result of crack entry invasion of emerging lateral roots, might also enter rice and wheat by a similar route. Following inoculation with ORS571 carrying a lacZ reporter gene, azorhizobia were observed microscopically within the cracks associated with emerging lateral roots of rice and wheat. A high proportion of inoculated rice and wheat plants had colonized lateral root cracks. The flavanone naringenin at 10 and 10 M stimulated significantly the colonization of lateral root cracks and also intercellular colonization of wheat roots. Naringenin does not appear to be acting as a carbon source and may act as a signal molecule for intercellular colonization of rice and wheat by ORS571 by a mechanism which is nod gene-independent, unlike nodule formation in Sesbania rostrata. The opportunity now arises to compare and to contrast the ability of Azorhizobium caulinodans with that of other rhizobia, such as Parasponia rhizobia, to intercellularly colonize the roots of non-legume crops.
    Materialart: Digitale Medien
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  • 49
    ISSN: 1573-5036
    Schlagwort(e): Acetobacter ; auxin ; 2 ; 4-D ; clover ; nitrogen fixation ; Rhizobium ; rice ; transgenic plants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Root morphology is both genetically programmed and environmentally determined. We have begun an analysis into the components of root development by: (a) constructing a range of transgenic clover plants to assess some of the genetic programs involved as both roots and nodules are initiated and develop. These transgenic plants report on auxin activity, flavonoid synthesis and chitinase expression and suggest a role for flavonoids as regulators of auxin levels; and (b) determining in cereals the effect of both added auxin and specific microorganisms on the initiation and development of modified root outgrowths and lateral roots. Appropriate combinations of auxin, the nitrogen fixing Acetobacter diazotrophicus, and rice variety did give rise to some plants which grew slowly for over 12 months in a nitrogen-free medium.
    Materialart: Digitale Medien
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  • 50
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 196 (1997), S. 201-210 
    ISSN: 1573-5036
    Schlagwort(e): leaf photosynthesis ; nitrogen nutrition ; ribulose-1 ; 5-bisphosphate carboxylase/oxygenase ; rice ; Rubisco ; yield potential
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Characteristics of rice (Oryza sativa) as a crop plant are briefly introduced, and the relationship between formation of yield potential and nitrogen (N) nutrition is described on the basis of studies using 15N as a tracer. In addition, the relationship between the leaf photosynthetic capacity and leaf N, and the factors limiting leaf photosynthesis under different growth conditions are reviewed. Finally, targets for improving rice yield potential are discussed with a focus on the role of increased photosynthesis efficiency in relation to leaf N status and the photosynthetic components in the leaves.
    Materialart: Digitale Medien
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  • 51
    ISSN: 1573-5036
    Schlagwort(e): nitrogen ; 13N ; 15N ; phloem sap ; positron-emitting tracer imaging system ; rice ; xylem
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Nitrogen movement through the xylem vessels and sieve tubes in rice plants was studied using xylem and phloem sap analysis in combination with stable and radioactive nitrogen isotope techniques. More than 90% of nitrogen was translocated in the sieve tubes of rice plants as amino acids. When 15N (99.6 atom%) was applied as a nitrate to the root, 15N first appeared in phloem sap of the leaf sheath within 10 minutes and increased to 37 atom% excess 5 hours after the experiment had started. In long-term experiments, 63% of nitrogen in the phloem sap of the leaf sheath and 15% in that of the uppermost internode came from nitrogen absorbed within the last 24 hours and 50 hours, respectively. To obtain information about the more rapid circulation of nitrogen in the plant, radioactive 13N was used as a tracer. A positron-emitting tracer imaging system was used to show that 13N was transferred to the leaf sheath within 8 minutes of its application to the roots. Analysis of the xylem sap of the leaf sheath showed that when the nitrate was applied to the roots, most of the nitrogen in the xylem was transported as a nitrate. These data showed that phloem and xylem sap analysis together with the stable and radioactive nitrogen techniques provide a good method for the detection of nitrogen cycles in plants.
    Materialart: Digitale Medien
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  • 52
    ISSN: 1573-5060
    Schlagwort(e): Oryza sativa ; rice ; aluminium-resistance ; selection ; stress ; in vitro ; hydroponic cultures
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A selection procedure is described that isolated Al-resistant individuals from a sensitive rice (Oryza sativa L.) genotype. Somaclonal variation was used as the only source of variability and selection pressures were applied at both callus and regenerated plant levels. When 10 week-old, embryo-derived calli were submitted to different Al stresses for a period of 20 weeks. After this in vitro stress period, selected calli were grown during 18 weeks, without selection pressure, on regeneration media. Selection pressures were applied on regenerated plants (R0), and 9 R0 plants which produced seeds were selected. The transmission of the Al-resistant character to the R1, R2, R3 and R4 generations was then investigated, and three plant lines which had an increased percentage of Al-resistant plants till the fourth generation of self-pollination, were identified. One was from a callus maintained on an Al-free medium. The efficiency of in vitro selection pressures was therefore debated.
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  • 53
    ISSN: 1573-5060
    Schlagwort(e): isozyme ; differentiation ; Oryza sativa ; rice ; upland rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Two rice ecotypes, the so-called lowland and upland populations, which carry different isozyme genotypes mostly at a single locus, are cultivated in Japan. The aim of this study was to examine the origin and the mechanism for keeping these genetic differences. The upland population is cultivated in upland fields and carries a different allele for a particular isozyme gene, Pgd-1, which has never been found in the lowland population. RFLP markers showed a weak trend for genetic differentiation between the two ecotypes. On the other hand, morphological, and physiological traits showed marked differences between the two ecotypes. Furthermore, based on the genotypic difference, two Japonica subgroups are defined in the upland population. Subgroup I is the minor group and carries key lowland characters, including the genotype for PGD. Subgroup II carries different traits and the genotype for PGD of the alternative subgroup. As an allelic difference for Pgd-1 is known to occur between the two ecospecies, Tropical (Tr) and Temperate (Tm) Japonicas, upland cultivars can be classified by diagnostic characters which distinguish a variety into Tr or Tm type. The upland population consists of three types of cultivars, Tr-, Tm- and intermediate-type. In contrast, the lowland population consists of a uniform Tm type Japonicas. As Japanese upland cultivars still have an isozyme allele specific to the Tr type, the upland population has a rather complex constitution which is presumably now being introgressed by lowland genetic material, but still represents a major difference at some genetic levels. Upland rice carries several stress-resistant genes which would be useful for lowland rice breeding. The genetic difference would be efficient for tagging upland specific traits by upland specific genetic markers.
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  • 54
    ISSN: 1573-5036
    Schlagwort(e): association ; clover ; endophyte ; PGPR ; Rhizobium leguminosarum bv. trifolii ; rice ; root ; symbiosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract For over 7 centuries, production of rice (Oryza sativa L.) in Egypt has benefited from rotation with Egyptian berseem clover (Trifolium alexandrinum). The nitrogen supplied by this rotation replaces 25- 33% of the recommended rate of fertilizer-N application for rice production. This benefit to the rice cannot be explained solely by an increased availability of fixed N through mineralization of N- rich clover crop residues. Since rice normally supports a diverse microbial community of internal root colonists, we have examined the possibility that the clover symbiont, Rhizobium leguminosarum bv. trifolii colonizes rice roots endophytically in fields where these crops are rotated, and if so, whether this novel plant-microbe association benefits rice growth. MPN plant infection studies were performed on macerates of surface-sterilized rice roots inoculated on T. alexandrinum as the legume trap host. The results indicated that the root interior of rice grown in fields rotated with clover in the Nile Delta contained ∼106 clover-nodulating rhizobial endophytes g fresh weight of root. Plant tests plus microscopical, cultural, biochemical, and molecular structure studies indicated that the numerically dominant isolates of clover-nodulating rice endophytes represent 3 – 4 authentic strains of R. leguminosarum bv. trifolii that were Nod Fix on berseem clover. Pure cultures of selected strains were able to colonize the interior of rice roots grown under gnotobiotic conditions. These rice endophytes were reisolated from surface-sterilized roots and shown by molecular methods to be the same as the original inoculant strains, thus verifying Koch's postulates. Two endophytic strains of R. leguminosarum bv. trifolii significantly increased shoot and root growth of rice in growth chamber experiments, and grain yield plus agronomic fertilizer N-use efficiency of Giza-175 hybrid rice in a field inoculation experiment conducted in the Nile Delta. Thus, fields where rice has been grown in rotation with clover since antiquity contain Fix strains of R. leguminosarum bv. trifolii that naturally colonize the rice root interior, and these true rhizobial endophytes have the potential to promote rice growth and productivity under laboratory and field conditions.
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  • 55
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 194 (1997), S. 161-169 
    ISSN: 1573-5036
    Schlagwort(e): chitin ; elicitor ; receptor ; rhizobium ; rice ; legume
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract This review focuses on a comparison of plant reception of chitin oligosaccharides by legumes and rice. Chitin oligosaccharides (dp=6-8) released from fungal pathogens induce plant defense reactions in rice, while lipo-chitin oligosaccharides (dp=4-5) induce the development of a new plant organ, the nodule, in legumes during infection by rhizobia. The former situation is pathogenic and the latter situation beneficial to the plant. However, these two systems do share some common features. We hypothesize that rice and legumes, as well as other plants, may possess members of an evolutionarily conserved family of chitin binding proteins. These proteins may play an important role in chitin reception and subsequent signal transduction. However, data support the idea that legumes may possess a second chitin binding receptor that shows a greater specificity for the lipo-chitin nodulation signals. The presence of this second receptor may be one of the key factors that distinguishes plants capable of nodulation by rhizobia (e.g., soybean) from those that cannot be nodulated (e.g., rice).
    Materialart: Digitale Medien
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  • 56
    ISSN: 1573-5036
    Schlagwort(e): colonization ; indole-3-acetic acid ; invasion ; Nod factors ; nod gene induction ; rhizobia ; rhizobial attachment ; rice ; thick short lateral roots ; trans-zeatin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Legume-rhizobial interactions culminate in the formation of structures known as nodules. In this specialized niche, rhizobia are insulated from microbial competition and fix nitrogen which becomes directly available to the legume plant. It has been a long-standing goal in the field of biological nitrogen fixation to extend the nitrogen-fixing symbiosis to non-nodulated cereal plants, such as rice. To achieve this goal, extensive knowledge of the legume-rhizobia symbioses should help in formulating strategies for developing potential rice-rhizobia symbioses or endophytic interactions. As a first step to assess opportunities for developing a rice-rhizobia symbiosis, we evaluated certain aspects of rice-rhizobia associations to determine the extent of predisposition of rice roots for forming an intimate association with rhizobia. Our studies indicate that: a. Rice root exudates do not activate the expression of nodulation genes such as nodY of Bradyrhizobium japonicum USDA110, nodA of R. leguminosarum bv. trifolii, or nodSU of Rhizobium. sp. NGR234; b. Neither viable wild-type rhizobia, nor purified chitolipooligosaccharide (CLOS) Nod factors elicit root hair deformation or true nodule formation in rice; c. Rhizobia-produced indole-3-acetic acid, but neither trans-zeatin nor CLOS Nod factors, seem to promote the formation of thick, short lateral roots in rice; d. Rhizobia develop neither the symbiont-specific pattern of root hair attachment nor extensive cellulose microfibril production on the rice root epidermis; e. A primary mode of rhizobial invasion of rice roots is through cracks in the epidermis and fissures created during emergence of lateral roots; f. This infection process is nod-gene independent, nonspecific, and does not involve the formation of infection threads; g. Endophytic colonization observed so far is restricted to intercellular spaces or within host cells undergoing lysis. h. The cortical sclerenchymatous layer containing tightly packed, thick walled fibers appears to be a significant barrier that restricts rhizobial invasion into deeper layers of the root cortex. Therefore, we conclude that the molecular and cell biology of the Rhizobium-rice association differs in many respects from the biology underlying the development of root nodules in the Rhizobium-legume symbiosis.
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  • 57
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 194 (1997), S. 1-10 
    ISSN: 1573-5036
    Schlagwort(e): endophyte ; legume-Rhizobium ; nif gene ; nitrogen fixation ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Recent advances in understanding symbiotic Rhizobium-legume interactions at the molecular level, the discovery of endophytic interactions of nitrogen-fixing organisms with non-legumes, and the ability to introduce genes into rice by transformation have stimulated researchers world wide to harness opportunities for nitrogen fixation and improved N nutrition in rice. In a think-tank workshop organized by IRRI in 1992, the participants reaffirmed that such opportunities do exist for cereals and recommended that rice be used as a model system. Subsequently, IRRI developed a New Frontier Project to coordinate the worldwide collaborative efforts among research centers committed to reducing dependency of rice on mineral N resources. An international Biological Nitrogen Fixation (BNF) working group was established to review, share research results/materials and to catalyze research. The strategies of enabling rice to fix its own N are complex and of a long-term nature. However, if achieved, they could enhance rice productivity, resource conservation, and environmental security. The rate of obtaining success would, of course, benefit tremendously from concerted efforts from a critical mass of committed scientists around the world, as well as a constant and continued funding support from the ‘donor’ community.
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  • 58
    ISSN: 1573-5036
    Schlagwort(e): endophytic microbes ; endo-symbiosis ; genomic fingerprinting ; infection ; marker genes ; nitrogen fixation ; PCR ; rep-PCR ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The extension of nitrogen-fixing symbioses to important crop plants such as the cereals has been a long-standing goal in the field of biological nitrogen fixation. One of the approaches that has been used to try to achieve this goal involves the isolation and characterization of stable endophytic bacteria from a variety of wild and cultivated rice species that either have a natural ability to fix nitrogen or can be engineered to do so. Here we present the results of our first screening effort for rice endophytes and their characterization using acetylene reduction assays (ARA), genomic fingerprinting with primers corresponding to naturally occurring repetitive DNA elements (rep-PCR), partial 16S rDNA sequence analysis and PCR mediated detection of nitrogen fixation (nif) genes with universal nif primers developed in our laboratory. We also describe our efforts to inoculate rice plants with the isolates obtained from the screening, in order to examine their invasiveness and persistence (stable endophytic maintenance). Lastly, we review our attempts to tag selected isolates with reporter genes/proteins, such as beta-glucuronidase (gus) or green fluorescent protein (gfp), in order to be able to track putative endophytes during colonization of rice tissues.
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  • 59
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 97 (1997), S. 25-29 
    ISSN: 1573-5060
    Schlagwort(e): Oryza sativa ; amylograph viscosity characteristics ; inheritance ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Southern United States long grain rice (Oryza sativa L.) is a unique market type characterized by intermediate amylose content, gelatinization temperature, and amylograph viscosity profile characteristics. Rice breeders sometimes use long grain germplasm with differing amylograph viscosity profiles. The inheritance of rice amylograph viscosity profiles has not been ascertained and, thus, was the objective of this study. A six parent diallel (parents plus F1 hybrids) was evaluated at Stuttgart, AR during 1994 and 1995. In 1994, 65 F2 plants from the ‘L-202’ × ‘Newrex’ and L-202 × ‘Katy’ crosses were space planted, and in 1995, the 65 F2:3 lines were planted as hill plots. The inheritance of amylograph viscosity profiles appeared to be controlled by a single locus with genes with additive effects. The results of the diallel analysis also support control by a single locus with genes with additive effects because GCA effects were significant and SCA effects nonsignificant for peak, hot paste, and cool paste viscosities. Intermediate amylose content and intermediate amylograph viscosity profiles were strongly correlated. Selection first for intermediate amylose content followed by reselection for intermediate hot paste viscosities would identify long grain genotypes with southern United States long grain cooking quality in progeny from crosses between southern United States long grain rice genotypes and softer cooking long grain genotypes.
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  • 60
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 189 (1997), S. 145-153 
    ISSN: 1573-5036
    Schlagwort(e): coleoptile ; cultivar ; direct sowing ; flooded soil ; rice ; seedling establishment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Seedling establishment of direct sown rice plants is less successful in flooded soil than in drained soil. This study was conducted to clarify the difference in morphogenesis of rice seeds sown in flooded and drained soils and to identify the morphological characteristics responsible for successful establishment of cultivars in flooded soil. Rice cultivars ASD1 and IR41996–50–2–1–3, superior in seedling establishment in flooded soil, and Mahsuri and IR72, non-superior (control), were sown at a depth of 25 mm in soil flooded with 25 mm of water or in drained soil. The coleoptile and 1st leaf emerged from the soil surface simultaneously in drained soil while in flooded soil the coleoptile emerged first. The coleoptile of superior cultivars, unlike the controls, elongated more in flooded soil than in drained soil. In flooded soil, the development of mesocotyl, 1st leaf, and roots were inhibited to a greater extent in the controls, than in the superior cultivars. In sealed flasks in which gas containing 0–21% O2 was exchanged daily, the superior cultivars developed longer coleoptiles than the controls at lower O2 concentrations. These findings suggest that the reason superior cultivars grow better in flooded soil than the controls is that the coleoptile elongates faster and longer in hypoxia and is able to reach the soil surface where O2 is available.
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  • 61
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 95 (1997), S. 253-258 
    ISSN: 1573-5060
    Schlagwort(e): rice ; Oryza sativa ; widely compatible restorer ; fertility restoration ability ; anther culture ; inheritance ; Indica-japonica heterosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Following anther culture of various F1 hybrids of indica restorer (R) lines/wide compatible varieties (WCVs) and japonica R lines/WCVs, the homozygous diploid plants (2n) generated were test-crossed with indica WA type cytoplasmic-genetic male sterile (CMS) line Zhanshan 97A (WA), indica testers Nanjing 11 and Nante, japonica BT CMS line Hanfeng A (BT), and japonica testers Balilla and Akihikari to identify widely compatible restorer lines. The results of this study showed that among the diploid pollen plants generated from F1 hybrids of indica R lines/WCVs, 36.7% and 64.7% possessed normal fertility restoration ability (rate of seed-setting 〉 80%) to Hanfeng A and Zhanshan 97A, respectively. 71.3% and 32.3% had normal fertility restoration ability to Hanfeng A and Zhanshan 97A, respectively, in diploid pollen plants derived from the japonica R lines/WCVs F1's anther culture. Several widely compatible R lines were selected from anther culture of F1 hybrids of indica R lines/WCVs and japonica R lines/WCVs. These widely compatible R lines derived from diploid pollen plants showed good wide compatibility and restoration ability both to WA and BT type CMS lines. Strong standard heterosis of major agronomic traits and yield traits was observed in F1 test-crosses of widely compatible R lines with Hanfeng A and Zhanshan 97A. The wide compatibility in widely compatible R lines H17, H158 and H281 was studied by a set of three-way crosses. Segregation of the fertile plants with seed-setting rate 〉 70% and semi-fertile plants with seed-setting rate 〉 69.9% agreed with a ratio of 1:1, indicating that wide compatibility in these widely compatible R lines is governed by a pair of major genes.
    Materialart: Digitale Medien
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  • 62
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 95 (1997), S. 301-307 
    ISSN: 1573-5060
    Schlagwort(e): gene location ; heading time ; Oryza sativa ; photoperiod sensitivity ; rice ; trisomic analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Recent genetic analyses on heading-time of rice indicated that almost all the well-adapted varieties in the temperate zone carry a strong photoperiod-sensitivity gene E1, a dominant allele of E1 locus. In order to identify the chromosome on which E1 is located, a trisomic analysis was made using two primary trisomic series originating from the japonica varieties, Nipponbare and Kinmaze, respectively. The Nipponbare and Kinmaze series were crossed with heading-time tester lines, EG0 and EG3, respectively, both of which did not carry the E1. The F2 populations for chromosome 1, 2, and 3 could not be analyzed due to lack of seed. All the other F2 populations showed distinct segregation into early-type and late-type plants caused by the E1 locus segregation, which suggested that the trisomic analysis for E1 locus could be efficiently made. Both disomic and trisomic groups in the F2 population from the cross of the trisomic line for chromosome 7 × EG0 showed a segregation ratio significantly different at the 1% level from a ratio of 1 [e1e1; early]: 3 [E1e1, E1E1; late]. This suggested that E1 was located on chromosome 7. Subsequently, the linkage analysis was made using three morphological marker genes on chromosome 7. It was recognized that E1 was linked to rfs (rolled fine strip gene) and slg (slender glume gene) with recombination values of 16.3 ± 5.88 and 9.1 ± 4.72%, respectively. From these results, it can be concluded that E1 is most likely to be located on chromosome 7.
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  • 63
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 96 (1997), S. 135-142 
    ISSN: 1573-5060
    Schlagwort(e): Oryza sativa ; chilling tolerance ; in vitro selection ; rice ; somaclonal variation ; field performance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Embryo-derived calli of four rice varieties cultivated at high altitude in Burundi — Facagro 57, Facagro 76, Kirundo 3 and Kirundo 9 — were submitted to different temperature regimes. The percentage of regenerating calli greatly varied depending on variety, length of culture and callus temperature treatment. The reduction of regeneration percentages induced by low temperature was more pronounced in the more sensitive varieties. Regenerated plants (R0) and their progenies in R1, R2 and R3 were cold-screened together with control plants. In all varieties, significantly higher survival rates were obtained in R3 with in vitro plants than with control plants. Such chilling tolerance improvement was not obtained following a massal selection applied during 3 successive generations onto the control plants. In vitro plants regenerated from calli cultivated either at 25 °C, either at 4 °C, were cultivated at different altitudes in Burundi during two successive generations. For most observed traits, the in vitro plants were characterized by lower means, larger variation and higher maximum values than the control plants. The most chilling-tolerant somaclonal families were most usually characterized by extensive differences in fatty acid composition, chilling-induced electrolyte leakage and chlorophyll fluorescence, compared to the varieties they derived from.
    Materialart: Digitale Medien
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  • 64
    ISSN: 1573-5060
    Schlagwort(e): Oryza sativa ; Aus ; rice ; hybrid sterility ; isozomes ; varietal diversity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Magnitudes of genetic variation within each of major varietal groups of cultivated rice were surveyed in terms of isozyme polymorphism and allelic differentiation of hybrid sterility loci, both of which are considered to have litt le selective value. Allelic differences for 20 isozyme loci were examined in a total of 337 accessions, including Indica and Japonica rices. Aus cultivars from India, Javanica cultivars and both landraces and cultivars from China. Eleven out of the 20 isozyme loci were polymorphic. The Aus cultivars contained more alleles per locus than others. The hybrid sterilities in the crosses among Chinese cultivars, Indica and Japonica cultivars were mainly controlled by locus S-5, whereas the hybrid sterilities of Aus cultivars, when they were crossed to Indica, Japonica or Javanica cultivars, were found to be controlled by allelic interaction at hybrid sterility loci S-5, S-7, S-9 and S-15. Also in terms of the number of alleles at S-5 and S-7, Aus cultivars contained more alleles than others. While the Aus group showed an extreme diversity for both hybrid sterility alleles and isozymes, modern cultivars from the south of YangZi River in China were classified into Indica type and those from the north were into Japonica, which were almost the same as those in Japan. On the basis of the measured polymorphism the Indica-Japonica differentiation was explained by founder effects, i.e., through selection and distribution of two original sources each with a unique set of markers. Contrastingly, the continuous and pronounced diversity in the Aus group was attributed to the absence of such a process. The intermediate groups in Yunnan province and Tai-hu Lake region of China are considered to be isolated from such founder effects, retaining an intermediate diversity in terms of isozymes and hybrid sterility genes. Since hybrid sterility hampers the exchange of genes between cultivars of different groups, the understanding of its genetic basis will be important in rice breeding, particularly in hybrid rice breeding.
    Materialart: Digitale Medien
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  • 65
    ISSN: 1572-9788
    Schlagwort(e): diversity ; genetic mapping ; Oryza sativa ; RAPDs ; rice ; inter-simple sequence repeats
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Genetic variation between samples of Oryza sativa from 19 localities in Bangladesh and Bhutan was assessed using two PCR-based molecular marker systems: RAPD (random amplification of polymorphic DNA) and ISSR-PCR (inter-simple sequence repeat polymerase chain reaction). Employing RAPD, a set of 14 decanucleotides of arbitrary sequence directed the amplification of 94 reproducible marker bands, 47 (50%) of which were polymorphic. In addition, a set of 9 ISSR primers were used to direct amplification of 71 PCR products, 40 (56%) of which were polymorphic. Multivariate analyses of the two PCR-based molecular marker data sets provided evidence that the patterns of variation correspond with the classification described by Glaszmann [9] using isozyme analysis. Subtle differences in the relationships revealed between rice groups using the two types of PCR-based marker led to investigations of their map positions using an intraspecific doubled haploid mapping population. The observation that the chromosomal locations of markers can influence diversity assessments is presented and the significance of this is discussed.
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  • 66
    ISSN: 1572-9788
    Schlagwort(e): Bacillus thuringiensis ; Chilo suppressalis ; biolistic ; rice ; Scirpophaga incertulas ; transformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Rice cultivars of isozyme group V include high-quality, aromatic rices that are difficult to improve by traditional methods because of the loss of quality characters upon sexual hybridization. Their low-tillering plant type predisposes them to economic loss from attack by stem borers, a group of insects to which they are susceptible. We report here the enhancement of stem borer resistance in cv. Tarom Molaii through transformation by microprojectile bombardment. Embryogenic calli derived from mature seeds were bombarded with gold particles coated with plasmid pCIB4421, carrying a synthetic truncated toxin gene based on the cryIA(b) gene from Bacillus thuringiensis, and plasmid pHygII, carrying the hygromycin phosphotransferase (hpt) selectable marker gene. Inclusion of 50 mg/l hygromycin B in culture media from bombardment through to rooting of plantlets eliminated escapes. The procedure generated three independent hpt transformants of which two also contained the cryIA(b) gene. One such line (No. 827) produced truncated (67 kDa) CryIA(b) protein equivalent to about 0.1% of total soluble protein. The cryIA(b) gene was controlled by the promoter of the maize C4 PEP carboxylase gene and was expressed in leaf blades but was not expressed to a detectable level in dehulled mature grain. Line 827 contained about 3 copies of the cryIA(b) gene which segregated as a single dominant Mendelian locus in the second (T1) and third (T2) generations and co-segregated with enhanced resistance to first-instar larvae of striped stem borer (Chilo suppressalis) and yellow stem borer (Scirpophaga incertulas). T2 line 827-6 homozygous for the cryIA(b) gene showed no dead hearts or whiteheads after infestation with stem borers, whereas T2 line 827-25 lacking the gene averaged 7 dead hearts per plant and 2.25 whiteheads per plant. These results establish that transformation of high-quality rices of group V is a feasible alternative to sexual hybridization.
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  • 67
    Digitale Medien
    Digitale Medien
    Springer
    Genetic resources and crop evolution 44 (1997), S. 159-164 
    ISSN: 1573-5109
    Schlagwort(e): genotypic differences ; germinability ; isozyme groups ; longevity ; rice ; seed development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The storage potential of seeds harvested at different stages of maturity was studied in ten cultivars of Oryza sativa, representing six known isozyme groups, and in two cultivars of O. glaberrima. Mass maturity (the end of the seed-filling period) was attained between 14.2 and 20.2 days after anthesis (DAA). A comparison of the estimates of p 50 (time in storage for viability to decline to 50%) of seeds harvested at 21, 28 and 35 DAA and stored at 35°C with 15% moisture content showed that maximum longevity was attained between 28 and 35 DAA in most cultivars. Cultivars belonging to isozyme group II survived longer than other cultivars with estimates of p 50 nearly doubled. On the other hand, the floating rices of Group IV had shorter longevity. Within group VI, the upland cultivar survived longer than the lowland cultivar. Both O. glaberrima cultivars survived reasonably well, showing that African rice cultivars also differ in longevity.
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  • 68
    Digitale Medien
    Digitale Medien
    Springer
    Irrigation and drainage systems 10 (1996), S. 95-107 
    ISSN: 1573-0654
    Schlagwort(e): automation ; percolation ; performance ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract This rapid appraisal study compares the actual and projected performance of a large, rice monoculture irrigation project in the humid climate of the Caspian Sea in Iran. The uniqueness of this project is the co-existence of East-Asia rice farming techniques with advanced technologies of automatic water control found generally in the Mediterranean countries. The paper emphasizes the need to do field tests at the design stage in order to make realistic assumptions on water efficiency and deep percolation. The study confirms that the advanced water control technologies are appropriate for small farm irrigation projects in the humid tropics, a conclusion often debated among experts. The Guilan project now provides some evidence to support that conclusion.
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  • 69
    Digitale Medien
    Digitale Medien
    Springer
    Irrigation and drainage systems 10 (1996), S. 193-205 
    ISSN: 1573-0654
    Schlagwort(e): canal irrigation ; design ; gates ; equitable distribution ; management ; rice ; Malaysia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A method is presented to analyse the impact of the selection of irrigation gates on operational performance of the Sungai Muda Irrigation Scheme in Malayisa. The method examines the discharge capacity of the water control gates at all levels in order to compare the specific water supply (the ratio of supply to command area) with the specific water demand which is the required hydromodule. The term hydromodule is the reciprocal of “water duty” and thus has units of litres/second/hectare. The greater the deviation between the two, the greater the potential loss of control during the operation of the scheme. The method is relatively simple but is more complex in this particular example as two hydromodules are used for the irrigation of basin rice; one for the presaturation period and one for the normal supply period. The most common cause of loss of water control is found to be provision of oversized turnout gates at the head of secondary and tertiary canals. Such design approximations enable more water to be used in those command areas thus leading to waste and to shortage of water in other areas. It is suggested that during design and rehabilitation of irrigation schemes, the operational implications of design approximations should be examined more carefully.
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  • 70
    ISSN: 1573-0867
    Schlagwort(e): long-term experiments ; potassium ; rice ; soil testing ; ion-exchange resin ; potassium-supplying capacity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Based on the analysis of soil K status in 11 long-term fertility experiments in Asia, two approaches for assessing the K-supplying power of lowland paddy soils were evaluated to predict total K uptake by irrigated rice. A regression model combining commonly used static soil test parameters that appeared to integrate measures of K release from nonexchangeable forms as well as chemical factors affecting K activity in soil solution explained 72% of the crop K uptake in NP and NPK treatments. However, this approach would require determination of six soil properties and it does not provide a direct measure of K release dynamics. The second approach used mixed-bed ion exchange resin capsules to measure K release during 2-wk anaerobic incubation. The resin method provided an integrative measure of soil K status and the factors controlling K transformation and diffusion rates, which are embodied in two kinetic parameters describing the dynamics of rapid- and slow-phase K release. The resin method was sensitive to past fertilizer history and the resulting buildup or depletion of soil K reserves and it was a better predictor of total K uptake in the NP and NPK plots (r2=0.82) than static soil tests. The results also indicated that high (Ca+Mg)/K ratios may contribute to K deficiency in rice soils.
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  • 71
    ISSN: 1573-0867
    Schlagwort(e): long-term experiments ; phosphorus ; rice ; nutrient balance ; phosphorus uptake ; fertilizer P response ; soil testing ; ion-exchange resin ; phosphorus supplying capacity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Data from long-term experiments at 11 sites in Asia with a wide range of nutrient input treatments and yield levels were used to quantify crop P requirements of rice (Oryza sativa L.) and the P balance in intensive, irrigated rice systems. Uptake of 1.8–4.2 kg P was required to produce one ton of grain yield. Physiological P use efficiency varied between 220 to 900 kg grain kg P-1. Without added P, there was a net loss of 7 to 8 kg P ha-1 per crop; with added P there was a net gain of 4 to 5 kg P ha-1 per crop. Phosphorus adsorption kinetics on mixed-bed ion-exchange resin capsules provided an integrative measure of soil P status, P diffusion, and acid-induced P solubilization. The resin capsule was a sensitive tool to characterize buildup or depletion of soil P as a result of different P balances. Both Olsen-P and the resin capsule were suitable methods to predict P uptake of tropical lowland rice. It is hypothesized that both methods measure a similar soil P pool which is soluble under alkaline, aerobic conditions but transformed into acid-soluble P froms as a result of submergence and reduction. Present recommendations for P fertilizer use on rice of 20–25 kg P ha-1 are adequate to maintain yields of 5–6 t ha-1, but sustaining higher yields of 7–8 t ha-1 will require farm-specific management strategies based on knowledge of the long-term P balance and soil P-supplying capacity.
    Materialart: Digitale Medien
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  • 72
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 46 (1996), S. 189-193 
    ISSN: 1573-0867
    Schlagwort(e): available P ; green manure ; P source ; P uptake ; residual effect ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract To evaluate residual recoveries of fertilizer phosphorus (P) two field experiments were conducted in Quezon Province, Philippines during 1991 dry season, under irrigated condition. Average grain yield increase was 0.50–0.9 t/ha due to residual effects of inorganic fertilizer P, regardless of source. Residual effects of fertilizer P with Sesbania rostrata or alone increased grain yield by 0.3–1.0 t/ha over control. Significantly higher yields were obtained with S. rostrata fertilized with Morocco phospate Rock (MPR) and S. rostrata + MPR applied on rice than the control. Results revealed that there is a promising effect of residual P from the applied P sources in increasing rice grain yield. Total P uptake increased due to residual P from fertilizer P applied. Results suggest that increased P uptake increased dry matter and grain yield.
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  • 73
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 46 (1996), S. 195-203 
    ISSN: 1573-0867
    Schlagwort(e): available phosphorus ; green manure ; phosphorus source ; phosphorus uptake ; relative effectiveness ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract To evaluate alternative fertilizer phosphorus (P) sources in lowland rice, two field experiments were conducted under irrigated conditions in Quezon Province, Philippines during 1990–1991 crop year. In another field experiment fertilizer P recycling through a green manure crop applied in the succeeding rice, was studied. Addition of fertilizer P increased grain yield by 1.5–2.0 t/ha (46%) in 1990 wet season (WS) and by 1.6–2.1 t/ha (56%) in 1991 dry season (DS). However, fertilizer P source and application level did not effect grain yield significantly. Results indicated that the less water-soluble and less expensive partially acidulated phosphate rock (PAPR), phosphate rock (PR) and less reactive PR were as effective as the more soluble but more expensive triple superphosphate (TSP). The relative effectiveness (RE) of local guano was significantly lower than that of other sources of fertilizer P. Fertilizer P applied to a pre-rice Sesbania rostrata green manure increased rice grain yield by 1.5–1.9 t/ha during 1991 DS. Further, S. rostrata fertilized with Morocco phosphate rock (MPR) gave significantly higher rice grain yield than did rice fertilized with MPR applied alone. In the P source experiments Olsen method and Pi correlated better with growth attributes than Bray 2 P. Phoshours uptake did not differ significantly among P sources and levels. Results suggest that P uptake was improved with green manuring. Correlation analyses revealed a close correlation between P uptake and dry matter yield and P uptake and grain yield.
    Materialart: Digitale Medien
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  • 74
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 46 (1996), S. 29-39 
    ISSN: 1573-0867
    Schlagwort(e): Côte d'Ivoire ; rice ; fertilizers ; adoption ; gender ; socioeconomic factors ; field factors ; markets ; population pressure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract This paper analyzed the factors that affect the adoption of chemical fertilizers by rice farmers in Côte d'Ivoire using a Tobit model. The results show that the major factors that positively influence farmers' use of fertilizers in rice fields are cultivation of lowlands, use of mechanization, farm size, land pressure and availability of non-farm income. Factors found to negatively affect the use of fertilizer in rice fields are the distance of the field to the village, distance of the village to the major market, and if the cultivator is a female. The paper concludes with strategies for targeting soil fertility interventions on farmers' rice fields.
    Materialart: Digitale Medien
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  • 75
    ISSN: 1573-0867
    Schlagwort(e): long-term experiments ; nutrient balance ; potassium ; rice ; potassium use efficiency ; potassium uptake ; potassium response
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Research in many countries indicates a negative K balance in intensive, irrigated rice systems but comparative studies across different environments are few. Using a uniform sampling methodology, we measured K uptake, K use efficiency, and K balance in six different fertilizer treatments of long-term fertility experiments with rice at 11 sites in five Asian countries. Depending on the absolute yield level, K uptake requirements of rice ranged from 17 to 30 kg K per ton of grain. For yields greater than 8 t ha-1, total K uptake exceeded 200 kg ha-1. The K balance at most experimental sites was negative, with an average net removal of 34–63 kg K season-1. There was significant depletion of soil K reserves at many sites. Based on these data, we estimated that the amount of K cycled annually from the soil into rice plants is 7–10 million t in irrigated rice systems of Asia. About 1 million t of this total amount is removed with the harvested grain. Present recommendations for K addition in most intensive irrigated rice domains are insufficient to replace K removal. However, response to K can only be expected on soils with deficient supply capacity and where other nutrients, particularly N and P, are not limiting. Efficient K management for rice must therefore be based on the K input/output balance, the achievable yield target, and the effective K-supplying power of the soil.
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  • 76
    ISSN: 1573-5087
    Schlagwort(e): mineral nutrition ; osmotic adjustment ; proline ; rice ; salt-resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Salt-resistant rice cultivars Nona Bokra and IR 4630 exposed at the seedling stage during one or two weeks to 0, 20, 30, 40 or 50 mM NaCl accumulated less Na, Cl, Zn and proline and more K at root and shoot levels than salt-sensitive I Kong Pao and IR 31785. Aiwu, a moderately resistant genotype, exhibited an intermediate behaviour. P transport from root to shoot was inhibited in the most sensitive cultivar IR 31785. Accumulation of Na and Cl and decrease in K content at the shoot level were restricted to the oldest leaves in salt-resistant genotypes while proline accumulated in the youngest leaves in all cultivars. In the presence of NaCl, the osmotic potentials of the roots and of the oldest and youngest leaves were lower in the salt-resistant than in the salt-sensitive genotypes, differences among genotypes increasing with stress intensity. Proline did not appear to be involved in osmotic adjustment in salt-stressed rice plants and the significance of its accumulation is discussed in relation to salinity resistance.
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  • 77
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 89 (1996), S. 349-354 
    ISSN: 1573-5060
    Schlagwort(e): Oryza sativa ; quantitative trait loci ; gene mapping ; restriction fragment ; length polymorphism ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Segregation of plant height (PH), tiller number (TN), panicle number (PN), average panicle length per plant (PL), average primary branch number per panicle per plant (PBN) and 1000 grain weight (1000G) were specific in an F2 population derived from a cross of Palawan, a tall Javanica variety, and IR42, an Indica semidwarf variety. One hundred and four informative RFLP markers covering all 12 chromosomes were used for detecting putative QTLs controlling the traits. Orthogonal contrasts and interval mapping analysis were used for the analysis. QTL detected for PH on the region of chromosome 1, where semidwarfing gene sd-1 locus is located, seems to be a multiple allelic locus. An additional QTL for PH was identified on chromosome 2. Two QTLs for TN were detected on chromosomes 4 and 12. The QTL on chromosome 4 seemed also to govern the variation in PN. Four QTLs were found for the other traits, two of them for PL were located on chromosomes 6 and 2, one for PBN on chromosome 6 and the other for 1000G on chromosome 1. Additive gene actions were found to be predominant, except one QTL for PH and one QTL for PL, but partial or incomplete dominance also existed for the QTLs detected.
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  • 78
    ISSN: 1573-5060
    Schlagwort(e): Oryza sativa ; inter-varietal cross ; spikelet fertility ; genetic basis ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary There is much interest in the inter-varietal fertility of Asian cultivated rice. In this study, we analyzed the fertility of hybrids in a diallel set of 210 crosses involving 21 parents representing a broad range of the cultivated rice germplasm including landraces, primitive cultivars, modern elite cultivars and parents of hybrid rice. The materials were also carefully selected with respect to indica-japonica classification including typical indica/japonica, more or less intermediate and wide compatibility varieties. The level of hybrid fertility varied widely among the crosses from almost completely sterile to fully fertile. In general, hybrid fertility of intra-subspecific crosses, i.e., indica by indica (I×I) and japonica by japonica (J×J), is much higher than inter-subspecific crosses (I×J or J×I). The fertility varied widely in hybrids involving wide compatibility varieties and also in inter-subspecific crosses not involving wide compatibility varieties. An analysis of variance showed that both the main effects of indica and japonica parents and the interaction between the parents are highly significant in determining hybrid fertility. We speculate that, in addition to wide compatibility, the overall genetic difference between the indica and japonica parents, that may involve a series of minor mutations, play an important role in determining the hybrid fertility. There are also genes of sizable effect that influence fertility in hybrids resulting from specific combinations of the parents.
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  • 79
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 91 (1996), S. 99-101 
    ISSN: 1573-5060
    Schlagwort(e): inheritance ; lipoxygenase-3 ; monoclonal antibody ; null-allele ; rice ; volatile compounds
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Lipoxygenase (LOX) catalyzes peroxidation of lipids in rice grains and is related to the formation of volatile compounds. We report on the mode of inheritance of LOX-3 in the rice variety Daw Dam. This variety lacks the LOX-3 (Suzuki et al., 1993), which is a major isozyme in rice seeds. The inheritance of LOX-3 in rice seeds was determined by crossing Daw Dam with rice cultivars having normal LOX-3 activity. The distribution of F2 phenotypes for the presence and absence of LOX-3 from selfed F1 plants fitted the ratio 3:1. Segregation ratio of the B1F1 generation gave a good fit to 1 presence of LOX-3: 1 absence. These data support the hypothesis that the lack of LOX-3 is recessive to the presence of LOX-3, and we conclude that the absence of LOX-3 is inherited as a single recessive trait. We discuss the possible use of the LOX-3 less mutation in relation to volatile compounds production in rice grains.
    Materialart: Digitale Medien
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  • 80
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 91 (1996), S. 285-288 
    ISSN: 1573-5060
    Schlagwort(e): anther culture ; breeding ; doubled haploids ; Oryza sativa ; rice ; salt tolerant
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary A haploid breeding program was initiated to develop doubled haploid salt tolerant rice breeding line via anther culture. Two sensitive breeding lines BR4608-R1-R2 and BR4909-R1-R2 were crossed with a salt tolerant line IR13146-13-3-3 to transfer its salt tolerant character to the doubled haploids. Anther from confirmed F1s of the two crosses were cultured in defined medium for callus induction and eventual plant regeneration. Fifteen doubled haploid (DH) lines were obtained from two crosses. Test for salt tolerance were done in vitro. Five out of 15 lines were found tolerant at the level of 8–10 decisiemens/m (ds/m) while the rests were sensitive to that level of salinity. Field experiment was conducted to evaluate the doubled haploids under saline and non saline soil. Five salt tolerant lines produced comparable yield with the resistant control (BR 23) under saline condition, whereas these lines yielded even higher in non saline soil under irrigated condition when evaluated with other 10 sensitive DH lines
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  • 81
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 180 (1996), S. 159-163 
    ISSN: 1573-5036
    Schlagwort(e): Fe2+ ; inductive mechanism ; peroxidase ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Peroxidase (POD) activity was significantly stimulated in detached rice leaves by Fe2+. By electric focusing with thin-layer scanning, three new bands were identified in Fe2+-treated leaves, and the activities of other isozymes were also drastically increased, of which a band with a pI of ca. 9.8 was enhanced from trace amount (invisible) in the control to a large band in the Fe2+-treated leaves. How Fe2+ induced POD activity was investigated. Cycloheximide completely blocked the stimulation of POD activity by Fe2+, but actinomycin D could not inhibit it effectively, indicating that the stimulated activity resulted from inducing de novo biosynthesis of the enzyme at translational level rather than at transcriptional level. When same molar concentration of H2O2 as Fe2+ and sublethal and lethal concentrations of paraquat were introduced to the rice leaves, no significant stimulation of POD activity was observed compared with Fe2+-induced activity, and scavengers of free radicals still could not inhibit the induction of POD by Fe2+. Several chemicals such as EGTA, LaCl3, CPZ, verapamil, which were known to interfere with the action of calcium/calmodulin, were not able to retard POD induction by Fe2+. From these results it appears that the process of Fe2+-induced POD may not be mediated by free radicals or calcium/calmodulin action.
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  • 82
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 88 (1996), S. 1-7 
    ISSN: 1573-5060
    Schlagwort(e): rice ; Oryza sativa ; hybrid rice ; thermosensitive genic male sterility (TGMS) ; rice genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Inheritance of thermosensitive genic male sterility (TGMS) in Norin PL12 and IR32364TGMS and their allelic relationship were studied from F1, F2 testcross (TC) and F3 generations of the crosses made with the two mutants and several fertile tester parents. F2, TC and F3 segregation behavior for pollen and spikelet fertility indicated that the TGMS trait in the two mutants was controlled by a single recessive gene. Allelic relationship studies indicated that TGMS genes of the two mutants were different. Since TGMS gene in Norin PL12 has been designated as tms 2 , the TGMS gene present in IR32364TGMS is tentatively designated as tms 3 (t) until allelic test is done with another TGMS gene (tms 1 ) reported from China in a line 5460S seeds of which were not available.
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  • 83
    ISSN: 1573-5060
    Schlagwort(e): Oryza sativa ; rice ; chilling ; electrolyte leakage ; chlorophyll fluorescence ; seed dormancy ; seed germination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Rice varieties were screened for chilling tolerance during germination and vegetative growth using different techniques. The effects of temperature during germination were investigated from 10 to 25°C. The screening was most effective at 10°C. Time of data collection has to be considered in order to discriminate slow germinating from chilling sensitive varieties. Chilling survival tests (10°C) applied at the 2-leaf stage revealed that different chilling durations brought complementary results: the longer treatments allowed to identify the most tolerant varieties, while shorter treatments allowed to distinguish intermediate from most sensitive rices. Results of chilling tests applied at the 2- and the 8-leaf stages showed a high correlation. Low-temperature electrolyte leakage and low-temperature fluorescence were also highly correlated to each other and to survival tests applied at the same development stage. By contrast, weak correlations were observed between low-temperature germination and plantlet chilling survival. It is concluded that low-temperature electrolyte leakage and low-temperature fluorescence are good indicators of chilling tolerance during the vegetative growth. The possibility to use a chilling screening procedure applied at this stage in order to predict the chilling tolerance at other development stages is discussed.
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  • 84
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 89 (1996), S. 243-248 
    ISSN: 1573-5060
    Schlagwort(e): rice ; blast ; Magnaporthe grisea ; partial resistance ; selection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Divergent selection for higher and lower partial resistance to leaf blast was applied in F2 populations of crosses between the rice cultivars IR36, IR64 and CO39 after exposure to a virulent isolate. IR36 and IR64 are partially resistant while CO39 is highly susceptible. As selection criteria the number of sporulating lesions in leaves of the main culm or the lesion density in the topmost leaf relative to other F2 plants with the same stage of development were used. A highly significant and meaningful response to selection was obtained in most cases, but the heritability was low. Realized heritabilities varied from 0.14 to 0.25 depending on the cross and were similar for both selection criteria. Selection for improved partial resistance to leaf blast is possible as early as the F2. The efficiency of selection is probably much higher if replicated tests could be made, and better results are therefore expected if selection among F3 lines is carried out. The results indicated that the relatively low infection efficiency in IR36 and IR64 is oligo- or polygenically controlled.
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  • 85
    ISSN: 1573-5060
    Schlagwort(e): heading time ; gene analysis ; Oryza sativa ; photoperiod insensitivity ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The release of extremely early maturing varieties has made it possible to cultivate rice in Hokkaido (NL45-42°) in Japan, the northern limit region of paddy rice cultivation. Until then, rice cultivation in this region has been impracticable due to the climate condition, especially short summer and long-day more than 15 hours during summer. Experiment results confirmed that the success of rice cultivation in this area depends on raising photoperiod insensitivity varieties with short basic vegetative growth period. Moreover, in this study, the genetic factors controlling the photoperiod insensitivity of Hokkaido varieties were analyzed by using 8 kinds of tester lines for three loci, E1, E2, and E3, controlling photoperiod sensitivity. It was found out that all the varieties examined carry el, a photoperiod insensitivity allele of E1 locus, but as for the other loci, E2 and E3, the existence of plural alleles were recognized. We have already clarified that almost all the japonica-type varieties grown in Japan (except Hokkaido) and Taiwan carry E1 bringing about strong photoperiod sensitivity. Accordingly, it can be concluded that el is the gene essential to Hokkaido varieties: the interchange of E1 with e1 enabled rice cultivation under long-day condition.
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  • 86
    Digitale Medien
    Digitale Medien
    Springer
    Molecular breeding 2 (1996), S. 267-276 
    ISSN: 1572-9788
    Schlagwort(e): Agrobacterium ; Javanica rice ; regeneration ; rice ; TAIL-PCR ; transformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Difficulties frequently encountered using direct DNA transfer methods for transformation of Javanica varieties of rice (Oryza sativa L.) have limited the application of biotechnology to these varieties. We now reportAgrobacterium-mediated transformation of Javanica cultivars Gulfmont and Jefferson that are, respectively, widely used or about to enter commercial cultivation in the southern USA. Vigorous, phenotypically normal, fertile plants expressing both the selectable marker and the gene of interest were obtained. Southern analysis showed that only one or two copies of the T-DNA insert were present. Sequence analysis of right border fragments of one line confirmed that insertion was into a coding region of rice nuclear DNA. This analysis also revealed the presence of relatively short regions of permuted T-DNA border sequences, similar to those found afterAgrobacterium-mediated transformation of dicots. Progeny analysis of lines bearing two copies showed co-segregation, indicating that they were located relatively closely on the same chromosome. The introduced genes were transmitted to the R1 and R2 generations in a Mendelian fashion, confirming the suitability of this approach for biotechnological improvement of elite rice cultivars.
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  • 87
    Digitale Medien
    Digitale Medien
    Springer
    Irrigation and drainage systems 9 (1995), S. 117-128 
    ISSN: 1573-0654
    Schlagwort(e): efficiency ; rice ; Senegal ; water use
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The water use of rice schemes was derived from field measurements made during the 1991 and 1992 season at 13 irrigation schemes situated in the delta and valley of the Senegal river. The seasonal water consumption, as well as the water distribution throughout the season and the operation of the pumping stations were evaluated. The water consumption was compared with standards which ranged from 16,650 to 21,100 m3 ha−1 depending on the season and the location of the scheme. Half of the analyzed schemes used more water than required. Analysis of the water distribution throughout the season revealed that in general the supply follows the demand. In small-scale schemes however, the fit between supply and demand is often lacking, resulting in crop stress and yield reduction. Furthermore, the study shows that the capacity of half of the pumping stations are under-used as a result of a long stretched saturation period and/or the irrigation of only a small fraction of the total area of the scheme.
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  • 88
    Digitale Medien
    Digitale Medien
    Springer
    Irrigation and drainage systems 9 (1995), S. 129-141 
    ISSN: 1573-0654
    Schlagwort(e): rice ; salinity ; Senegal ; water requirements
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The paper estimates the water requirements for salt control in rice schemes located on saline soils in the Senegal river delta. When the fields are not cultivated, salts are transported to the top soil by capillary rise from the very saline and shallow ground water table. During the irrigation season, the large quantity of irrigation water adds additional salt to the fields. If the percolation rate of the soil is small, salts will have to be removed from the schemes by flushing the standing water from the fields when the salinity of the water reaches the threshold value of 1.5 ds m−1. The results indicate that if the schemes are located on the river banks (fondé), flushing at the beginning of the season and the percolation losses throughout the season may be sufficient to keep the salts out of the root zone. On the less permeable soils in the depressions (hollaldé), an extra flushing is required to evacuate enough salts from the fields during the irrigation season. In total about 2,300 m3 ha−1 of water may be needed for flushing. If flushing is not practised, the schemes have to be abandoned after a few years of cultivation due to build-up of soil salinity.
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  • 89
    Digitale Medien
    Digitale Medien
    Springer
    Irrigation and drainage systems 9 (1995), S. 371-387 
    ISSN: 1573-0654
    Schlagwort(e): agricultural production ; Bihar ; India ; irrigation ; performance ; rice ; production function ; canals ; tubewells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Despite considerable discussion of the importance of timeliness as a key dimension of irrigation performance, few studies have assessed how well irrigation systems deliver timely water supplies, nor the magnitude of the effect on agricultural production. This paper lays out indicators of timeliness of irrigation supply which distinguish between deliveries which meet crop needs, and surplus water supplies which cannot be used by crops. These indicators are then applied to empirical data from the Sone Irrigation System of Bihar, India. Using these indicators in an analysis of the contribution of irrigation to rice production shows that incorporating measures of timeliness explains much more of the variability in agricultural production than do simple measures of total water applications over a season. Results of production functions show that if water deliveries cannot be matched with crop requirements, they have a negative, rather than a positive, impact on yields. Water scarcity has the greatest adverse impact in production in the middle of the season, while surpluses are most damaging at the beginning and end of the season. Temporal redistribution from surplus periods to times of water scarcity therefore offers considerable scope to increase productivity without increasing water use.
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  • 90
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 41 (1995), S. 67-75 
    ISSN: 1573-0867
    Schlagwort(e): additives ; agronomic efficiency ; P availability ; phosphate rocks ; rice ; (Oryza sativa L.) ; thermally altered rock P
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The P availability in soil and agronomic efficiency of the products of non-premium grade, unreactive Purulia phosphate rock (PPR) heated alone or with Na2CO3 or KCl at different temperatures were investigated in two P deficient soils. The heated products of PPR alone did not improve the P availability in soil or P utilisation by rice over the original PPR. The products of PPR-KCl mixtures heated at 300-900°C were not effective at all. Out of several products of PPR with Na2CO3, the product prepared from PPR and Na2CO3 mixture in the weight ratio 2:1 heated at 900°C was comparable to superphosphate (SP) with respect to P availability in soil, straw and grain yield and P uptake by rice. The effectiveness of the products of PPR-Na2CO3 mixtures heated at 700°C though inferior to SP were superior to that of the original PPR in the highly acidic P deficient soil from Choudwar. However, products of another phosphate rock (PR) from Jordan and NA2CO3 mixtures heated at 900°C were less effective in comparison to SP. The amount of inherent silica present in Jordan PR was inadequate to promote the apatite-NA2CO3-SiO2 reaction towards completion thus leading to an inferior product. On the other hand, similar products of non-premium grade Kasipatnam and Mussoorie PRs which are not suitable for direct application were comparable to SP in their effectiveness when these PRs were fused with Na2CO3 in the weight ratio 2:1 at 900°C. X-ray diffraction studies indicated presence of water and citrate soluble phosphate phases viz., Na3PO4, NaCaPO4 and possibly Ca7 (PO4)2 (S104)2 in these products of PR-Na2CO3 mixture heated at 900°C. The water and citrate soluble phases of these products could release adequate P for absorption by crop.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 91
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 42 (1995), S. 261-276 
    ISSN: 1573-0867
    Schlagwort(e): Azolla ; BNF ; cyanobacteria ; green manure ; heterotrophic bacteria ; legume ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The review summarizes the current status of the utilization of N2-fixing organisms as biofertilizer in rice cultivation. Heterotrophic bacteria, free-living cyanobacteria,Azolla, and legume green manures are considered with regard to their potential for increasing yield, their current use and the prospects for their use with regard to the identified limiting factors. Biological N2 fixation has been the most effective system for sustaining production in low-input traditional rice cultivation. On the other hand, the utilisation of N2-fixing organisms in intensified rice production encounters serious limitations. The utilization of free-living bacteria and cyanobacteria is refrained by their modest potential and the non establishment of inoculated strains.Azolla and legumes used as green manures have a high potential as N source, but their utilization is severely limited by socio-economic factors.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 92
    ISSN: 1573-5036
    Schlagwort(e): alley cropping ; Chromolaena odorata ; Gliricidia sepium ; groundnut ; maize ; rice ; root length density
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Tree root systems may improve soil fertility through carbon inputs, uptake of leachable nutrients and maintenance of soil biomass, but can at the same time reduce crop yields by competition for water and nutrients. Quantitative information about the positive and negative effects of tree roots and their changes in space and time are necessary for the optimization of agroforestry associations. An alley cropping experiment was layed out as a randomized complete block design on a Plinthic Lixisol/Ferralic Cambisol with Gliricidia sepium hedgerows at 5 m distance, including a sole cropping control. The development of root systems was monitored by sequential soil coring (eight samplings) during one year, with maize and groundnut as crops. Additional information is presented from a single sampling for rice during the foregoing year. Pronounced fluctuations of live root length density indicated an important variability in the nutrient and water uptake capacity of the vegetation. At low total root length density, the hedgerows affected the root development in the agroforestry plots directly by the presence of their root systems. At high root length density, they affected root development mainly by improving crop root growth and influencing the composition of the spontaneous vegetation. The root length density of the hedgerows was too low to compete with the crops for soil resources. The hedgerows tended to increase root length densities in the subsoil when few roots were present, thus possibly reducing the risk of nutrient leaching. However, the length density of the perennial root systems decreased during the cropping season, presumably as an effect of repeated pruning, and attained minimum values almost at the same time as the crops. Trees with denser root systems which are less frequently pruned may be more efficient in achieving closer nutrient cycles, though at the cost of higher root competition with crops.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 93
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 171 (1995), S. 317-322 
    ISSN: 1573-5036
    Schlagwort(e): halophytes ; rice ; salinity ; screening
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A number of varieties of rice, a halophyte, Sesuvium portulacastrum and a glycophyte, Phaseolus vulgaris were grown in culture solution containing a range of concentrations of NaCl. Growth of the plants and internal sodium concentrations of the roots were measured after 14 days. The electrical potential difference (PD) between the external solution and the vacuole of the outer cells of the root was also measured. This enabled the driving force on sodium at the cell membranes to be calculated using the Nernst equation. It was found that Sesuvium and those varieties of rice that had previously shown salt tolerance generated relatively negative PDs and large driving forces tending to exclude sodium from the root. This suggested that a simple measurement of PD for plants grown in a given concentration of NaCl over a given period of time would provide a fairly rapid screening method for salt tolerance in rice and possibly other species also. T J Flowers Section editor
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 94
    ISSN: 1573-5036
    Schlagwort(e): Bradyrhizobium japonicum ; ecology ; N2-fixation ; nodulation ; rice ; soybean
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The effect of rice culture on changes in the number of a strain of soybean root-nodule bacteria, (Bradyrhizobium japonicum CB1809), already established in the soil by growing inoculated soybean crops, was investigated in transitional red-brown earth soils at two sites in south-western New South Wales. At the first site, 5.5 years elapsed between the harvest of the last of four successive crops of soybean and the sowing of the next. In this period three crops of rice and one crop of triticale were sown and in the intervals between these crops, and after the crop of triticale, the land was fallowed. Before sowing the first rice crop, the number of Bradyrhizobium japonicum was 1.32×105 g−1 soil. The respective numbers of bradyrhizobia after the first, second and third rice crops were 4.52 ×104, 1.26×104 and 6.40×102 g−1 soil. In the following two years the population remained constant. Thus sufficient bradyrhizobia survived in soil to nodulate and allow N2-fixation by the succeeding soybean crop. At the second site, numbers of bradyrhizobia declined during a rice crop, but the decline was less than when the soil was fallowed (400-fold cf. 2200-fold). Multiplication of bradyrhizobia was rapid in the rhizosphere of soybean seedlings sown without inoculation in the rice bays. At 16 days after sowing, their numbers were not significantly different (p〈0.05) from those in plots where rice had not been sown. Nodulation of soybeans was greatest in plots where rice had not been grown, but yield and grain nitrogen were not significantly different (p〈0.05). Our results indicate that flooding soil has a deleterious effect on the survival of bradyrhizobia but, under the conditions of the experiments, sufficient B. japonicum strain CB 1809 survived to provide good nodulation after three crops of rice covering a total period of 5.5 years between crops of soybean.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 95
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 172 (1995), S. 123-129 
    ISSN: 1573-5036
    Schlagwort(e): cropping system ; green manuring ; mungbean ; nitrogen economy ; residue ; rice ; Sesbania aculeata L. ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Field experiments were carried out to determine the effect of Sesbania aculeata L. green manuring and mungbean, Vigna radiata (L.) residue incorporation on the response of rice to urea-N and their residual effects on a subsequent spring wheat. Compared with a pre-rice fallow, Sesbania green manuring and mungbean residue increased grain yield of rice by 0.4 and 0.3 t ha−1, respectively and of spring wheat by 0.6 and 0.7 t ha−1, respectively when no urea-N was applied to rice and 40 kg urea-N ha−1 as a basal starter dose was applied to wheat. Sesbania green manure and mungbean residue substituted for 43 and 30 kg urea-N ha−1 in rice and subsequently gave a beneficial effect in spring wheat equal to the residual effect of 89 and 112 kg urea-N ha−1 applied to rice, respectively. Mungbean residue remaining after the picking of pods, was found to be at par with Sesbania green manuring towards N contribution to “rice-wheat” cropping system but had an additional advantage of 0.5 to 1.3 t ha−1 seed yield of protein rich mungbeans.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 96
    ISSN: 1573-5036
    Schlagwort(e): endophytic microbes ; endo-symbiosis ; Rhizobium ; rice ; root hypertrophies ; ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract It has been a long-standing goal in the field of biological nitrogen fixation to extend nitrogen-fixing symbioses to presently non-nodulated cereal plants, such as rice. A number of researchers have recently described the induction of “nodule-like” structures on the roots of cereals primarily by rhizobia, in either the presence or absence of plant cell-wall-degrading enzymes or plant hormones. We briefly review this research and discuss the potential problems associated with the introduction of nitrogen-fixing microbes in novel physiological environments, such as rice roots. The results of experiments carried out in China on the induction of “nodule-like” structures on rice roots by rhizobia are highlighted. In addition, we present preliminary results of a series of experiments designed to repeat and evaluate these results using a variety of microscopic techniques and molecular genetic approaches.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 97
    ISSN: 1573-5036
    Schlagwort(e): abscisic acid ; humidity effects ; rice ; root-to-shoot communication ; soil salinity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract In a phytotron experiment four rice varieties (Pokkali, IR 28, IR 50, IR 31785-58-1-2-3-3) grown in individual pots were subjected to low (40/55% day/night) and high (75/90%) air humidity (RH), while soil salinity was gradually increased by injecting 0, 30, 60 or 120 mM NaCl solutions every two days. Bulk root and stem base water potential (SWP), abscisic acid (ABA) content of the xylem sap and stomatal resistance (rs) of the youngest fully expanded leaf were determined two days after each salt application. The SWP decreased and xylem ABA and rs increased throughout the 8 days of treatment. The effects were amplified by low RH. A chain of physiological events was hypothesized in which high soil electric conductivity (EC) reduces SWP, followed by release of root-borne ABA to the xylem and eventually resulting in stomatal closure. To explain varietal differences in stomatal reaction, supposed cause and effect variables were compared by linear regression. This revealed strong differences in physiological reactions to the RH and salt treatments among the test varieties. Under salt stress roots of IR 31785-58-1-2-3-3 produced much ABA under low RH, but no additional effect of low RH on rs could be found. By contrast, Pokkali produced little ABA, but rs was strongly affected by RH. RH did not affect the relationships EC vs. SWP and SWP vs. ABA in Pokkali, IR 28, and IR 50, but the relationship ABA vs. rs was strongly affected by RH. In IR 31785-58-1-2-3-3 RH strongly affected the relationship SWP vs. ABA, but had no effect on ABA vs. rs and EC vs. rs. The results are discussed regarding possible differences in varietal stomatal sensitivity to ABA and their implications for varietal salt tolerance.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 98
    ISSN: 1573-5060
    Schlagwort(e): rice ; Oryza sativa ; cytoplasmic male sterility ; fertility restoration ; chromosomal gene location ; primary trisomics ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Identification and location of fertility restoring genes facilitates their deployment in a hybrid breeding program involving cytoplasmic male sterility (CMS) system. The study aimed to locate fertility restorer genes of CMSWA system on specific chromosomes of rice using primary trisomics of IR36 (restorer), CMS (IR58025A) and maintainer (IR58025B) lines. Primary trisomic series (Triplo 1 to 12) was crossed as maternal parent with the maintainer line IR58025B. The selected trisomic and disomic F1 plants were testcrossed as male parents with the CMS line IR58025A. Plants in testcross families derived from disomic F1 plants (Group I crosses) were all diploid; however, in the testcross families derived from trisomic F1 plants (Group II crosses), some trisomic plants were observed. Diploid plants in all testcross families were analyzed for pollen fertility using 1% IKI stain. All testeross families from Group I crosses segregated in the ratio of 2 fertile: 1 partially fertile+partially sterile: 1 sterile plants indicating that fertility restoration was controlled by two independent dominant genes: one of the genes was stronger than the other. Testcross families from Group II crosses segregated in 2 fertile: 1 partially fertile+ partially sterile: 1 sterile plants in crosses involving Triplo 1, 4, 5, 6, 8, 9, 11 and 12, but families involving triplo 7 and triplo 10 showed significantly higher X2 values, indicating that the two fertility restorer genes were located on chromosome 7 and 10. Stronger restorer gene (Rf-WA-1) was located on chromosome 7 and weaker restorer gene (Rf-WA-2) was located on chromosome 10. These findings should facilitate tagging of these genes with molecular markers with the ultimate aim to practice marker-aided selection for fertility restoration ability.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 99
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 173 (1995), S. 21-28 
    ISSN: 1573-5036
    Schlagwort(e): bronzing ; iron toxicity ; rice ; stress ethylene
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The relationship among iron toxicity, bronzing symptom, and stress-induced ethylene production (SEP) was investigated in detached rice (Oryza sativa L.) leaves during the vegetative-ripening stage and in whole plants during the vegetative stage. When Fe2+ (200 mg L-1) was applied to the detached leaf through a transpiration stream, SEP was higher in the first leaf than in the second and third leaves from the top and maximal around the panicle primordia initiation stage. The genotype difference in SEP was more pronounced in the second and third leaves than in the first leaf. Bronzing intensity increased as SEP increased; iron concentration increase during treatment in the tissue did not correlate with bronzing intensity or with SEP among the 16 genotypes tested. When the roots of an intact plant were exposed to 300 mg L-1 of Fe2+ in culture solution little stress-induced ethylene was produced. By partially or totally derooting the plant, however, stress-induced ethylene was evoked, indicating that roots reduced the Fe2+ uptake so that little stress ethylene is produced in the intact plant. Leaf tissue tolerance for Fe2+ may contribute to genotype differences in iron toxicity tolerance of rice plants when roots are injured during transplanting or exposed to toxic substances in the soil.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 100
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 173 (1995), S. 239-250 
    ISSN: 1573-5036
    Schlagwort(e): fertility ; Oryza sativa ; pollen viability ; rice ; salinity ; seed set ; stigma ; stigmatic receptivity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The effect of salinity on the reproductive physiology of five rice genotypes (IR54, IR26, IR2153-26-3-5-2, IR15324-117-3-2-2 and BR6), was investigated by treatment from panicle initiation with sodium concentrations of 20, 35 or 50 mol m-3 in an ‘artificial seawater’. In an experiment conducted in a glasshouse, plant height and dry weight were little affected by the treatments. There was, however, genotypic variation in the extent of the sodium accumulation, with IR15324-117-3-2-2 containing the highest and IR2153-26-3-5-2 the lowest concentrations: sodium concentrations were higher in older than younger leaves. Salinity delayed flowering, reduced the number of productive tillers, the number of fertile florest per panicle, the weight per grain and the grain yield: effects on grain yield were very much more severe than on vegetative growth. Panicle length was also reduced as was the number of primary branches in a panicle: again there was genotypic variation in the response of these characters to salinity, with the number of branches in IR2153-26-3-5-2 being particularly sensitive. The concentration of sodium increased in the pollen, stigmas, lemmas and paleas with each increment of external salinity. The highest concentrations of sodium in pollen and stigmas was recorded in IR54 and IR15324-117-3-2-2. Pollen viability, whether tested with the tetrazolium salt thiazolyl blue (3-{4,5-dimethylthiazolyl-2}-2,5-diphenyl monotetrazolium bromide or MTT), germination on stigmas, growth through the stylar tissue or F1 seed set, was reduced particularly in those genotypes accumulating most sodium. At all three salt levels, a genotype which accumulated more Na in its pollen produced less-viable pollen than those with less Na in their pollen. Since the amount of Na in the pollen was highly correlated with the Na in the flag leaf, assessment of flag leaf Na should prove a useful indicator of the likely pollen viability. Stigmatic receptivity was also reduced, when estimated either from germination of viable pollen on stigmas of salt-grown plants, its growth through the stylar tissue or F1 seed set. The reduction of seed set in crosses suggested that the overall consequences of salinity are dominated by effects on panicle development, stigmas and grain filling rather than on pollen. Analysis of the data suggests that genotypic variation exists in the extent to which salinity affects aspects of the plants reproductive physiology and development: this variation might be used in attempts to enhance the resistance of rice to salinity.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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