ISSN:
1573-5060
Keywords:
anther culture
;
breeding
;
doubled haploids
;
Oryza sativa
;
rice
;
salt tolerant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary A haploid breeding program was initiated to develop doubled haploid salt tolerant rice breeding line via anther culture. Two sensitive breeding lines BR4608-R1-R2 and BR4909-R1-R2 were crossed with a salt tolerant line IR13146-13-3-3 to transfer its salt tolerant character to the doubled haploids. Anther from confirmed F1s of the two crosses were cultured in defined medium for callus induction and eventual plant regeneration. Fifteen doubled haploid (DH) lines were obtained from two crosses. Test for salt tolerance were done in vitro. Five out of 15 lines were found tolerant at the level of 8–10 decisiemens/m (ds/m) while the rests were sensitive to that level of salinity. Field experiment was conducted to evaluate the doubled haploids under saline and non saline soil. Five salt tolerant lines produced comparable yield with the resistant control (BR 23) under saline condition, whereas these lines yielded even higher in non saline soil under irrigated condition when evaluated with other 10 sensitive DH lines
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00033089
Permalink
