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  • 1
    ISSN: 1432-2048
    Keywords: Nitrogen fixation ; Nodule development ; Senescence (nodules) ; Vigna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cowpea (Vigna unguiculata (L.) Walp cv. Vita 3) seedlings inoculated with Rhizobium strain CB756 were cultured with their root systems maintained in air or in Ar: O2 (80:20, v/v) during early nodule development (up to 24 d after sowing). Compared with those in air, seedlings in Ar:O2 showed progressive N deficiency with inhibited shoot growth, reduced ribulose-1,5-bisphosphate carboxylase and total protein levels and loss of chlorophyll in the leaves. Nodule initiation, differentiation of infected and uninfected nodule tissues and the ultrastructure of bacteriod-containing cells were similar in the air and Ar: O2 treatments up to 16 d after sowing. Thereafter the Ar: O2 treatment caused cessation of growth and development of nodules, reduced protein levels in bacteroids and nodule plant cells, and progressive degeneration of nodule ultrastructure leading to premature senescence of these organs. Provision of NO 3 - (0.1–0.2 mM) to Ar: O2-grown seedlings overcame the abovementioned consequences of N2 deficiency on nodule and plant growth, but merely delayed the degenerative effects of Ar: O2 treatment on nodule structure and senescence. Treatment of Ar: O2-grown seedlings with NO 3 - greatly increased the protein level of nodules but the increase was largely restricted to the plant cell fraction as opposed to the bacteroids. By contrast, NO 3 - treatment of air-grown seedlings increased protein of bacteroid and host nodule fractions to the same relative extents when compared with air-grown plants not supplemented with NO 3 - . These findings, taken together with studies of the distribution of N in nodules of symbiotically effective plants grown from 15N-labeled seed, indicate that direct incorporation of fixation products by bacteroids may be a critical feature in the establishment and continued growth of an effective symbiosis in the cowpea seedling.
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  • 2
    ISSN: 1432-2048
    Keywords: Nectary, extrafloral ; Vigna (nectaries)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nectar was collected from the extrafloral nectaries of leaf stipels and inflorescence stalks, and phloem sap from cryopunctured fruits of cowpea plants. Daily sugar losses as nectar were equivalent to only 0.1–2% of the plant's current net photosynthate, and were maximal in the fourth week after anthesis. Sucrose:glucose:fructose weight ratios of nectar varied from 1.5:1:1 to 0.5:1:1, whereas over 95% of phloem-sap sugar was sucrose. [14C]Sucrose fed to leaves was translocated as such to nectaries, where it was partly inverted to [14C]glucose and [14C]fructose prior to or during nectar secretion. Invertase (EC 3.2.1.26) activity was demonstrated for inflorescence-stalk nectar but not stipel nectar. The nectar invertase was largely associated with secretory cells that are extruded into the nectar during nectary functioning, and was active only after osmotic disruption of these cells upon dilution of the nectar. The nectar invertase functioned optimally (phloem-sap sucrose as substrate) at pH 5.5, with a starting sucrose concentration of 15% (w/v). Stipel nectar was much lower in amino compounds relative to sugars (0.08–0.17 mg g-1 total sugar) than inflorescence nectar (22–30 mg g-1) or phloem sap (81–162 mg g-1). The two classes of nectar and phloem sap also differed noticeably in their complements of organic acids. Xylem feeding to leaves of a range of 14C-labelled nitrogenous solutes resulted in these substrates and their metabolic products appearing in fruit-phloem sap and adjacent inflorescence-stalk nectar. 14C-labelled asparagine, valine and histidine transferred freely into phloem and appeared still largely as such in nectar. 14C-labelled glycine, serine, arginine and aspartic acid showed limited direct access to phloem and nectar, although labelled metabolic products were transferred and secreted. The ureide allantoin was present in phloem, but absent from both types of nectar. Models of nectary functioning are proposed.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 174 (1995), S. 83-101 
    ISSN: 1573-5036
    Keywords: management ; legumes ; rhizobia ; symbiotic N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Atmospheric N2 fixed symbiotically by associations between Rhizobium spp. and legumes represents a renewable source of N for agriculture. Contribution of legume N2 fixation to the N-economy of any ecosystem is mediated by: (i) legume reliance upon N2 fixation for growth, and (ii) the total amount of legume-N accumulated. Strategies that change the numbers of effective rhizobia present in soil, reduce the inhibitory effects of soil nitrate, or influence legume biomass all have potential to alter net inputs of fixed N. A range of management options can be applied to legumes growing in farming systems to manipulate N2 fixation and improve the N benefits to agriculture and agroforestry.
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  • 4
    ISSN: 1573-5036
    Keywords: intercropping ; N2 fixation ; natural15N abundance ; ureides ; Vigna umbellata ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The yield of N in maize (Zea mays L.) and ricebean (Vigna umbellata [Thumb.] Ohwi and Ohashi) were compared on a Tropoqualf soil in North Thailand in 1984 and 1985. Both species were grown in field plots in monoculture or as intercrops at a constant planting density equivalent to 8 maize or 16 ricebean plants per m2. The contribution of symbiotic N2 fixation to ricebean growth was estimated from measurements of the natural abundance of15N (δ15N) in shoot nitrogen and from analysis of ureides in xylem sap vacuumextracted from detached stems. The natural abundance of15N in the intercropped ricebean was found to be considerably less than that in monoculture in both growing seasons. Using maize and a weed (Ageratum conyzoides L.) as non-fixing15N reference plants the proportions (P 15N) of ricebean shoot N derived from N2 fixation ranged from 0.27 to 0.36 in monoculture ricebean up to 0.86 when grown in a 75% maize: 25% ricebean intercrop. When glasshouse-derived calibration curves were used to calculate plant proportional N2 fixation (Pur) from the relative ureide contents of field collected xylem exudates, the contribution of N2 fixation to ricebean N yields throughout the 1985 growing season were greater in intercrop than in monocrop even at the lowest maize:legume ratio (25∶75). Seasonal patterns of sap ureide abundance indicated that N2 fixation was greatest at the time of ricebean podset. The averagePur andP 15N in ricebean during the first 90 days of growth showed identical rankings of monocrop and intercrop treatments in terms of N2 fixation, although the two sets ofP values were different. Nonetheless, seasonal estimates of N2 fixation during the entire 147 days of legume growth determined from ureide analyses indicated that equivalent amounts of N could be fixed by ricebean in a 75∶25 intercrop and in monoculture despite the former being planted at one-quarter the density.
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  • 5
    ISSN: 1573-5036
    Keywords: legumes ; N2 fixation ; 15N analysis ; ureides ; xylem exudate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Methods for partitioning the nitrogen assimilated by nodulated legumes, between nitrogen derived from soil sources and from N2 fixation, are described as applied in peninsular Malaysia. The analysis of nitrogenous components translocated from the roots to the shoots of nodulated plants in the xylem sap is outlined, with some precautions to be observed for applications in the tropics. Some examples of the use of the technique in surverying apparent N2 fixation by tropical legumes, in studying interrow cropping in plantation systems and in assessing effects of experimental treatments on N2 fixation by food legumes, are described. Techniques for assesing N2 fixation by means of15N abundance have been used to show that applications of nitrogenous fertilizers commonly used in Malaysia for soybeans depress N2 fixation, that similar results are obtained with natural abundance and15N-enrichment methods and that, in at least two locations in Malaysia, differences between the natural abundance of15N in plant-available soil nitrogen and in atmospheric N2 are great enough to permit application to measurement of N2 fixation by leguminous crops.
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  • 6
    ISSN: 1573-5036
    Keywords: Bradyrhizobium japonicum ; ecology ; N2-fixation ; nodulation ; rice ; soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of rice culture on changes in the number of a strain of soybean root-nodule bacteria, (Bradyrhizobium japonicum CB1809), already established in the soil by growing inoculated soybean crops, was investigated in transitional red-brown earth soils at two sites in south-western New South Wales. At the first site, 5.5 years elapsed between the harvest of the last of four successive crops of soybean and the sowing of the next. In this period three crops of rice and one crop of triticale were sown and in the intervals between these crops, and after the crop of triticale, the land was fallowed. Before sowing the first rice crop, the number of Bradyrhizobium japonicum was 1.32×105 g−1 soil. The respective numbers of bradyrhizobia after the first, second and third rice crops were 4.52 ×104, 1.26×104 and 6.40×102 g−1 soil. In the following two years the population remained constant. Thus sufficient bradyrhizobia survived in soil to nodulate and allow N2-fixation by the succeeding soybean crop. At the second site, numbers of bradyrhizobia declined during a rice crop, but the decline was less than when the soil was fallowed (400-fold cf. 2200-fold). Multiplication of bradyrhizobia was rapid in the rhizosphere of soybean seedlings sown without inoculation in the rice bays. At 16 days after sowing, their numbers were not significantly different (p〈0.05) from those in plots where rice had not been sown. Nodulation of soybeans was greatest in plots where rice had not been grown, but yield and grain nitrogen were not significantly different (p〈0.05). Our results indicate that flooding soil has a deleterious effect on the survival of bradyrhizobia but, under the conditions of the experiments, sufficient B. japonicum strain CB 1809 survived to provide good nodulation after three crops of rice covering a total period of 5.5 years between crops of soybean.
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  • 7
    ISSN: 1573-5036
    Keywords: Azolla ; Casuarina ; legume ; nitrogen fertilizer ; rhizobia ; symbiotic N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A fundamental shift has taken place in agricultural research and world food production. In the past, the principal driving force was to increase the yield potential of food crops and to maximize productivity. Today, the drive for productivity is increasingly combined with a desire for sustainability. For farming systems to remain productive, and to be sustainable in the long-term, it will be necessary to replenish the reserves of nutrients which are removed or lost from the soil. In the case of nitrogen (N), inputs into agricultural systems may be in the form of N-fertilizer, or be derived from atmospheric N2 via biological N2 fixation (BNF). Although BNF has long been a component of many farming systems throughout the world, its importance as a primary source of N for agriculture has diminished in recent decades as increasing amounts of fertilizer-N are used for the production of food and cash crops. However, international emphasis on environmentally sustainable development with the use of renewable resources is likely to focus attention on the potential role of BNF in supplying N for agriculture. This paper documents inputs of N via symbiotic N2 fixation measured in experimental plots and in farmers' fields in tropical and temperate regions. It considers contributions of fixed N from legumes (crop, pasture, green manures and trees), Casuarina, and Azolla, and compares the relative utilization of N derived from these sources with fertilizer N.
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  • 8
    ISSN: 1573-5036
    Keywords: humid tropics ; N balance ; N2 fixation ; N fertilizer ; nodulation ; rice ; rotation ; soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We report a study in northern Thailand to examine the effects of fertilizer N, applied both to paddy rice and to a subsequent soybean crop on symbiotic and yield characteristics of soybean and on the differences between inputs of fixed N2 and the removal of N as harvested product. Treatments were a factorial arrangement of 0, 100 and 300 kg N ha-1 applied to the rice (designated R0, R100 and R300, respectively), and 0,25 and 50 kg N ha-1, applied as ‘starter’ fertilizer to the soybean (S0, S25 and S50, respectively). Nitrogen applied to the rice increased rice yields by up to 74% but proportions recovered by the rice were low (45% [R100] and 14% [R300]). The rice N treatments had only marginal effects on soybean nodulation (up to 17% reduction in early growth) and above-ground dry matter (up to 9% increase). Effects on soybean seed yield and total N2 fixed were insignificant. Starter N, applied to the soybean at sowing, also marginally reduced nodulation and enhanced above-ground dry matter. Total N2 fixed was unaffected but seed yield was increased by up to 6%. For all treatments, total above-ground N ranged from 145 to 179 kg ha-1 with 72 to 85% (122 and 140 kg ha-1) derived from N2 fixation. When harvested product consisted of seed only, differences between inputs of fixed N2 and removals of seed N were close to zero (-10 to+9 kg N ha-1) with little effect of fertilizer N. The N balances were reduced by an average of 18 kg N ha-1 when straw was included as harvested product. We concluded that N applied to the rice and to the following soybean was inefficiently used by those crops and had only marginal effects of symbiotic activity of the soybean. Furthermore, the benefit of the N2 fixing soybean in this system was to slow the decline of, rather than enhance, the N fertility of the soil
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  • 9
    Publication Date: 1996-01-01
    Print ISSN: 0361-5995
    Electronic ISSN: 1435-0661
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 10
    Publication Date: 2003-06-01
    Print ISSN: 0178-2762
    Electronic ISSN: 1432-0789
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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