Summary
Rice varieties were screened for chilling tolerance during germination and vegetative growth using different techniques. The effects of temperature during germination were investigated from 10 to 25°C. The screening was most effective at 10°C. Time of data collection has to be considered in order to discriminate slow germinating from chilling sensitive varieties. Chilling survival tests (10°C) applied at the 2-leaf stage revealed that different chilling durations brought complementary results: the longer treatments allowed to identify the most tolerant varieties, while shorter treatments allowed to distinguish intermediate from most sensitive rices. Results of chilling tests applied at the 2- and the 8-leaf stages showed a high correlation. Low-temperature electrolyte leakage and low-temperature fluorescence were also highly correlated to each other and to survival tests applied at the same development stage. By contrast, weak correlations were observed between low-temperature germination and plantlet chilling survival. It is concluded that low-temperature electrolyte leakage and low-temperature fluorescence are good indicators of chilling tolerance during the vegetative growth. The possibility to use a chilling screening procedure applied at this stage in order to predict the chilling tolerance at other development stages is discussed.
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Björkman, O., 1987. Low-temperature chlorophyll fluorescence in leaves and its relationship to photon yield of photosynthesis in photoinhibition. In: D.J. Kyle, C.B. Osmond & C.J. Arntzen (Eds). Photoinhibition. pp. 123–144. Elsevier Science Publishers, Amsterdam.
Dagnelie, P., 1975. Théories et méthodes statistiques. Applications agronomiques. Vol. 2. Les Presses Agronomiques de Gembloux (Ed), Belgium.
Futsuhara, Y. & K. Toriyama, 1966. Genetic studies on cool tolerance in rice. III. Linkage relations between genes controlling cold tolerance and marker genes Nagao and Takahasi. Jpn. J. Breed. 16: 231–242.
Futsuhara, Y. & K. Toriyama, 1969. Genetic studies on cool tolerance in rice. IV. Direct and indirect effects of selection and cold tolerance. Jpn. J. Breed. 19: 286–292.
Futsuhara, Y. & K. Toriyama, 1971. Genetic studies on cool tolerance in rice. V. Effectiveness of individual and line selections for cool tolerance. Jpn. J. Breed. 21: 181–188.
Herner, R.C., 1990. The effects of chilling temperatures during seed germination and early seedling growth. In: C.Y. Wang (Ed). Chilling Injury of Horticultural Crops. pp. 51–69. CRC Press, Florida.
Hetherington, S.E., R.M. Smillie, A.K. Hardacre & H.A. Eagles, 1983. Using chlorophyll fluorescence in vivo to measure the chilling tolerances of different populations of maize. Aust. J. Plant Physiol. 10: 247–256.
Huang, L.-K., C.B. Osmond & I. Terashima, 1989. Chilling injury in mature leaves of rice. II. Varietal differences in the response to interaction between low temperature and light measured by chlorophyll fluorescence at 77 K and the quantum yield of photosynthesis. Aust. J. Plant Physiol. 16: 339–352.
Kaw, R.N. & G.S. Khush, 1985. A modified technique of screening for cold tolerance in rice. Int. Rice Res. Newsl. 10 (3): 15–16.
Kaw, R.N. & G.S. Khush, 1986. Combining ability for low temperature tolerance in rice. In: Rice Genetics. pp. 593–612. International Rice Research Institute, Manila, Philippines.
Khan, D.R., D.J. Mackill & B.S. Vergara, 1986. Selection for tolerance to low temperature-induced spikelet sterility at anthesis in rice. Crop Sci. 26: 694–698.
Krishnasamy, V.,& D.V. Seshu, 1989. Seed germination rate and associated characters in rice. Crop Sci. 29: 904–908.
Li, C.C. & J.N. Rutger, 1980. Inheritance of cool-temperature seedling vigor in rice and its relationship with other agronomic characters. Crop Sci. 20: 295–298.
Lyons, J.M., 1973. Chilling injury in plants. Annu. Rev. Plant Physiol. 24: 445–466.
Majumder, M.K., D.V. Seshu & V.V. Shenoy, 1989. Implication of fatty acids and seed dormancy in a new screening procedure for cold tolerance in rice. Crop Sci. 29: 1298–1304.
McRae, E.A., A.K. Hardacre & I.B. Ferguson, 1986. Comparison of chlorophyll fluorescence with several other techniques used to assess chilling sensitivity in plants. Physiol. Plant 67: 659–665.
Murata, N., 1983. Molecular species composition of phosphatidyl-glycerols from chilling-sensitive and chilling-resistant plants. Plant Cell Physiol. 24: 81–86.
Murata, N., N. Sato, N. Takahashi & Y. Hamazaki, 1982. Compositions and positional distributions of fatty acids in phospholipids from leaves of chilling-sensitive and chilling-resistant plants. Plant Cell Physiol. 23: 1071–1079.
Murata, N. & J. Yamaya, 1984. Temperature-dependent phase behavior of phosphatidylglycerols from chilling-sensitive and chilling-resistant plants. Plant Physiol. 74: 1016–1024.
Murata, T., 1990. Relation of chilling stress to membrane permeability. In: C.Y. Wang (Ed). Chilling Injury of Horticultural Crops. pp. 201–209. CRC Press, Florida.
Nagamine, T., 1991. Genetic control of tolerance to chilling injury at seedling stage in rice, Oryza sativa L. Jpn. J. Breed. 41: 35–40.
Nagamine, T. & M. Nakagahra, 1990. Genetic variation of chilling injury at seedling stage in rice, Oryza sativa L. Jpn. J. Breed. 40: 449–455.
Nagato, Y., N. Ueno, H. Hayasaka & K. Hinata, 1992. Growth response of radicle to low temperature in rice (Oryza sativa L.). Jpn. J. Breed. 42: 245–253.
Nizigiyimana, A., 1993. Détermination et caractérisation des phases de sensibilité aux basses températures chez le riz (Oryza sativa L.). Ph.D. Thesis, Université Catholique de Louvain, Belgium.
Norman, H.A., C. McMillan & G.A. Thompson, 1984. Phosphatidyl-glycerol molecular species in chilling-sensitive and chilling-resistant populations of Avicennia germinans (L.). Plant Cell Physiol. 25: 1437–1444.
Ögren, E. & G. Öquist, 1984. Photoinhibition of photosynthesis in Lemna gibba as induced by the interaction between light and temperature. III. Chlorophyll fluorescence at 77 K. Physiol. Plant. 62: 193–200.
Osmond, C.B., G.-Y. Ben, L.-K. Huang & T.D. Sharkey, 1987. Determining the role of light and stress effects on photosynthesis. In: D.D. Randall (Ed). Current Topics in Plant Biochemistry and Physiology. Vol. 6, pp. 134–146. Interdisciplinary Plant Group, University Missouri Press, Columbia.
Patterson, B.D., T. Murata & D. Graham, 1976. Electrolyte leakage induced by chilling in Passiflora species tolerant to different climates. Aust. J. Plant Physiol. 3: 435–442.
Paull, R.E., 1981. Temperature-induced leakage from chilling-sensitive and chilling-resistant plants. Plant Physiol. 68: 149–153.
Roughan, P.G., 1985. Phosphatidylglycerol and chilling sensitivity in plants. Plant Physiol. 77: 740–746.
Saltveit, M.E. & L.L. Morris, 1990. Overview on chilling injury of horticultural crops. In: C.Y. Wang (Ed). Chilling Injury of Horticultural Crops. pp. 3–15. CRC Press, Florida.
Sasaki, T., 1974. Studies on germination ability under low temperature condition of rice varieties. Kamikawa Agric. Exp. Sta., Hokkaido 24: 1–90.
Shahi, B.B. & G.S. Khush, 1986. Genetic analysis of cold tolerance in rice. In: Rice Genetics. pp. 429–435. International Rice Research Institute, Manila, Philippines.
Smillie, R.M. & R. Nott, 1979. Assay of chilling injury in wild and domestic tomatoes based on photosystem activity of the chilled leaves. Plant Physiol. 63: 796–801.
Smillie, R.M., S.E. Hetherington, J. He & R. Nott, 1988. Photoinhibition at chilling temperatures. Aust. J. Plant Physiol. 15: 207–222.
Sthapit, B.R., 1992. Genetic variation of chlorophyll synthesis of etiolated Nepalese rice seedlings at low temperature: a new approach for cold tolerance screening. Int. Rice Res. Newsl. 17 (6): 14–15.
Sthapit, B.R. & J.M. Wilson, 1992. Chilling tolerance in February seeded Chaite rices (Oryza sativa) of Nepal. Ann. Appl. Biol. 121: 189–197.
Sthapit, B.R., J.R. Witcombe & J.M. Wilson, 1995. Methods of selection for chilling tolerance in Nepalese rice by chlorophyll fluorescence analysis. Crop Sci. 35: 90–94.
Toriyma, K. & Y. Futsuhara, 1960. Genetic studies on cool tolerance in rice. I. Inheritance of cool tolerance. Jpn. J. Breed. 10: 143–152.
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Bertin, P., Kinet, J.M. & Bouharmont, J. Evaluation of chilling sensitivity in different rice varieties. Relationship between screening procedures applied during germination and vegetative growth. Euphytica 89, 201–210 (1996). https://doi.org/10.1007/BF00034606
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DOI: https://doi.org/10.1007/BF00034606