Publication Date:
1990-03-23
Description:
Cell cycle-regulated gene expression is essential for normal cell growth and development and loss of stringent growth control is associated with the acquisition of the transformed phenotype. The selective synthesis of histone proteins during the S phase of the cell cycle is required to render cells competent for the ordered packaging of replicating DNA into chromatin. Regulation of H4 histone gene transcription requires the proliferation-specific promoter binding factor HiNF-D. In normal diploid cells, HiNF-D binding activity is regulated during the cell cycle; nuclear protein extracts prepared from normal cells in S phase contain distinct and measurable HiNF-D binding activity, while this activity is barely detectable in G1 phase cells. In contrast, in tumor-derived or transformed cell lines, HiNF-D binding activity is constitutively elevated throughout the cell cycle and declines only with the onset of differentiation. The change from cell cycle-mediated to constitutive interaction of HiNF-D with the promoter of a cell growth-controlled gene is consistent with, and may be functionally related to, the loss of stringent cell growth regulation associated with neoplastic transformation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Holthuis, J -- Owen, T A -- van Wijnen, A J -- Wright, K L -- Ramsey-Ewing, A -- Kennedy, M B -- Carter, R -- Cosenza, S C -- Soprano, K J -- Lian, J B -- New York, N.Y. -- Science. 1990 Mar 23;247(4949 Pt 1):1454-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2321007" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
Cell Cycle/*genetics
;
Cell Line, Transformed
;
Cells, Cultured
;
DNA/genetics
;
DNA-Binding Proteins/*metabolism
;
Gene Expression Regulation, Neoplastic
;
Histones/*genetics
;
Humans
;
Molecular Sequence Data
;
Nuclear Proteins/*metabolism
;
*Promoter Regions, Genetic
;
RNA, Messenger/analysis
;
Rats
;
Sequence Homology, Nucleic Acid
;
Transcription, Genetic
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
