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  • Calcium
  • Lepidoptera
  • 1985-1989  (81)
  • 1980-1984  (49)
  • 1950-1954
  • 1940-1944
  • 1986  (81)
  • 1981  (49)
Collection
Publisher
Years
  • 1985-1989  (81)
  • 1980-1984  (49)
  • 1950-1954
  • 1940-1944
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Chemie 112 (1981), S. 149-156 
    ISSN: 1434-4475
    Keywords: Calcium ; Gallium ; Oxygen ; Single Crystal ; X-Ray
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The hitherto unknown compound Ca3Ga4O9 was prepared and investigated by X-ray single crystal methods. Ca3Ga4O9 has orthorhombic symmetry:a=1435.8;b=1682.5;c=532.1 pm; space group C 2v 11 −Cmm2,Z=6. The tetrahedra network (circles of 4 and 5 GaO4-tetrahedra) and the surrounding of Ca2+ are described and discussed with respect to other oxogallates.
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  • 2
    ISSN: 1570-7458
    Keywords: host-plant relations ; host variation ; Malacosoma americanum ; Malacosoma disstria ; Lasiocampidae ; Geometridae ; Lepidoptera ; niche breadth ; specialization ; plantinsect interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Raupen von Malacosoma americanum (F.), einer oligophagen Art, die sich vor allem auf Prunus und andern baumartigen Rosaceen entwickelt, wurden verglichen mit Raupen der polyphageren Verwandten M. disstria Hb. und zwar im Hinblick auf deren Empfindlichkeit auf Unterschiede im Blatt ihrer gemeinsamen Wirtspflanze, Prunus serotina Ehrh. Das Puppengewicht und die Entwicklungszeit bis zur Verpuppung wurden gemessen bei Raupen, welche auf Blättern von freiwachsenden und von beschatteten Jungpflanzen gezüchtet worden waren. Die Blattunterschiede hatten eine ausgesprochene Wirkung, aber es gab keine Unterschiede in der Reaktion der beiden Arten. Dieser Vergleich lässt vermuten, dass die Empfindlichkeit auf intraspezifische Unterschiede der Wirtspflanzenqualität bei wirtsspezifischen und polyphagen Arten gleich ist. Indessen dürften sich laut anderen Resultaten einige Insektenarten anders verhalten.
    Notes: Abstract Larvae of Malacosoma americanum (F.)(Lepidoptera: Lasiocampidae) an oligophagous species that feeds primarily on Prunus and other rosaceous trees, were compared to larvae of the more highly polyphagous congener M. disstria Hb., with respect to their sensitivity to variation in the foliage of a common host plant, Prunus serotina Ehrh. Pupal weight and time to pupation were measured on larvae reared on foliage from open-grown and from shaded saplings. The difference in foliage had a pronounced effect, but no difference was evident between the species in their response to the variation in foliage. This comparison implies that sensitivity to intraspecific variation in host quality does not differ between host-specific and generalized species. However, results from other species suggest that some species of insects do differ in this respect.
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  • 3
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    Entomologia experimentalis et applicata 30 (1981), S. 106-110 
    ISSN: 1570-7458
    Keywords: assimilation efficiency ; growth efficiency ; niche breadth ; specialization ; hostplant relations ; plant-insect interactions ; Malacosoma americanum ; Malacosoma disstria ; Lasiocampidae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Raupen von Malacosoma americanum, einer oligophagen Art, die sich vor allem auf Prunus und andern baumartigen Rosaceen. entwickelt, wurden mit Raupen der polyphageren Verwandten M. disstria in Hinblick auf die Verwertung der Blätter ihres gemeinsames Wirtes Prunus serotina verglichen. Wirerhielten ähnliche Werte wie sie früher für andere Lepidopteren publiziert worden waren und zwar in Bezug auf die üblichen Messwerte, Anteil verwertete Nahrung, Wachstum in Trockengewicht pro Einheit gefressene oder verwertete Nahrung. Zudem unterschieden sich die beiden Arten in keiner Masszahl für Effizienz. Unsere Resultate sind im Einklang mit der Folgerung, das spezialisierte phytophage Insekten ihre Wirtspflanzen nicht besser ausnützen als as polyphage Arten tun.
    Notes: Abstract Larvae of Malacosoma americanum (F.) an oligophagous species that feeds primarily on Prunus and other rosaceous trees, were compared to larvae of the more highly polyphagous congener M. disstria Hb., with respect to the efficiency of utilization of the foliage of a common host plant, Prunus serotina Ehrh. We obtained values similar to those reported for other Lepidoptera for the commonly used measures of the fraction of ingested food that was assimilated, and for the growth in dry weight per unit of food ingested or assimilated. Moreover, the two species did not differ in any measure of efficiency. Our results are compatible with the conclusion that specialized phytophagous insects do not use their host plants with greater physiological efficiency than do generalized insects.
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  • 4
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    Entomologia experimentalis et applicata 30 (1981), S. 280-292 
    ISSN: 1570-7458
    Keywords: Allozyme variation ; Lepidoptera ; Yponomeuta padellus ; evolutionary stages ; F-statistics ; panmixis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Le degré de différenciation génétique en allozymes à des niveaux divers de divergence évolutive (populations conspécifiques, sibling species et non-sibling species) d'Yponomeuta a été déterminé. Les distances génétiques entre des sibling species ou des non-sibling species sont très larges. Il en est conclu que de telles estimations ne fournissent pas beaucoup d'informations sur le phénomène de spéciation même. Des coéfficients de reproduction consanguine (F ST) ont été calculés pour des populations d'Y. padellus provenant de six plantes-hôtes. La valeur moyenne F STest .030±.012. Les valuers de F ST des populations recueillies sur Crataegus, (plante-hôte habituelle d'Y. padellus), sont inférieures 2–3.5 fois à celles des populations de l'ensemble des autres plantes-hôtes. L'apparition de races en fonction de l'hôte, mesurée par les différences importantes dans de fréquences des allozymes entre populations sympatriques sur plusieurs plantes-hôtes, a été examinée dans quatre régions. Il apparaît ainsi que la formation de races en fonction de l'hôte se produit chez Y. padellus et que la spéciation sympatrique est un évènement très vraisemblable.
    Notes: Abstract The amount of genetic differentiation at various levels of evolutionary divergence (conspecific populations, sibling species and non-sibling species) in Yponomeuta was determined. Genetic distances between siblings or non-siblings were found to cover a wide range. It is concluded that such estimates do not give much information on the speciation process itself. Inbreeding coefficients were calculated for populations of Y. padellus from a total of six host plants. The grand mean F ST value is 0.030±0.012. F ST values for populations sampled from Crataegus, the common food plant of Y. padellus, are 2–3.5 times smaller than those for populations from the other food plants taken together. Host race formation, as measured by significant differences in allozyme frequencies between sympatric populations on two or more food plants, was investigated in four areas. Host race formation seems to occur in Y. padellus and sympatric speciation is a likely event.
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  • 5
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    Entomologia experimentalis et applicata 40 (1986), S. 13-24 
    ISSN: 1570-7458
    Keywords: Acrolepiopsis assectella ; lépidoptère ; phytophage spécialiste ; électroantennogrammes ; substances volatiles ; composés soufrés ; Allium ; plantes non-hôtes ; Acrolepiopsis assectella ; Lepidoptera ; phytophagous speciality ; electroantennograms ; volatiles ; sulphur compounds ; Allium ; non-host plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary Electroantennogram studies of adults Acrolepiopsis assectella Zell., Lepidoptera, Hyponomeutoidea, showed that the insects responded to the host-plant's odour whether or not the plant was healthy or damaged by the larvae. The antenna of both sexes also responded to the host-plant's specific volatile sulphur compounds. All these odours attracted the insects in the olfactometer. Propyl propane thiosulfinate (Ti-P2), the most abundant of the host specific compounds was always the most effective. The antenna was also sensitive to certain non-host plants (cabbage and tomato). The insect antenna was also sensitive to compounds of the ‘general green-leaf volatil complex’ which were identified in leek (Allium) odour. The most effective of these, cis-hexen-3-ol-1, was also the only attractive one. Sensitivity to these different olfactory stimuli was similar for 1-day-old females and 5-days-old males. The insect's sensitivity to green-leaf compounds increased with age for both sexes, only males responded better to Ti-P2 with age. These same males were also more attracted by leek odour at 5 days old. These facts confirm the predominant role of the sulphur compounds in host finding by the leek moth. The exact biological effect of green-leaf compounds and the interference exerted by non-host plants odours such as tomato on attraction to leek is discussed.
    Notes: Résumé Les réponses E.A.G. d'Acrolepiopsis assectella Zell., Lépidoptère Hyponomeutoïdea spécialiste des Allium, montrent une sensibilité à l'odeur de la plante-hôte larvaire A. porrum saine ou attaquée, et à ses composés volatils soufrés spécifiques, toutes odeurs actives dans l'attraction des deux sexes en olfactomètre. Le Ti-P2, le plus abondant et le plus spécifique du végétal est toujours le plus efficace. Ceci confirme le rôle prépondérant des substances allélochimiques soufrées dans la recherche de la plante-hôte. Une réactivité antennaire plus ou moins importante est également enregistrée avec l'odeur de certaines plantes non-hôtes (chou et tomate). L'antenne du lépidoptère est également très sensible à certains produits volatils du ‘complexe odorant vert’ des végétaux qui sont identifiés dans l'odeur du poireau, le cis-hexen-3-ol-1 le plus actif est aussi le seul à être attractif. Le rôle biologique exact des ‘composés verts’ et l'interférence de l'odeur complète de la plantehôte avec celle de plantes non-hôtes, telle que la tomate, efficace sur l'antenne et sur le comportement sont discutés.
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  • 6
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    Entomologia experimentalis et applicata 41 (1986), S. 173-177 
    ISSN: 1570-7458
    Keywords: ascorbic acid analogs ; Spodoptera littoralis ; Lepidoptera ; moths reproduction ; spermatophore malformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les examens ont porté sur l'action des acides D-ascorbique, L-déhydroascorbique, 6-chloro-6-déoxy-L-ascorbique, 6-bromo-6-déoxy-L-ascorbique et l'ascorbyl-2-sulfate per os. Les actions de ces analogues de l'acide ascorbique ont été comparées à celles de témoins alimentés avec vitamine C et glucose. L'acide D-ascorbique a provoqué la formation de spermatophores anormaux sans qu'il y ait eu réduction de l'activité sexuelle. Les femelles inséminées par ces mâles ont pondu des oeufs stériles, et leur fécondité était inférieure à celles de femelles alimentées sur des régimes contenant des analogues L de l'acide ascorbique ou du sucrose. L'acide 6-chloro-6-déoxy-L-ascorbique et l'ascorbyl-2-sulfate ont réduit la fécondité et la fertilité mais ces analogues n'avaient pas altéré la formation des spermatophores. Les consommations des différents analogues proposés séparément par microalimentation ou au moment de l'accouplement ne diffèrent pas significativement. La discussion a porté sur l'action des analogues dextrogyres sur le spermatophore et l'utilisation de ces analogues dans la protection des cultures.
    Notes: Abstract D-ascorbic acid, L-dehydroascorbic acid, 6-chloro-6-deoxy-L-ascorbic acid, 6-bromo-6-deoxy-L-ascorbic acid and ascorbyl-2-sulfate were bioassayed per os on moths of Spodoptera littoralis (Boisd.). The activity of each of the analogs was compared with that of vitamin C and sucrose controls. D-ascorbic acid caused males to produce malformed spermatophores with no reduction in mating capacity. Females mated to these males layed infertile eggs and fecundity was lower than in females fed other analogs, L-ascorbic acid or sucrose diets. 6-chloro-6-deoxy-L-ascorbic acid reduced fertility and fecundity of the females, although this and other analogs did not affect the spermatophores. D-isoascorbic acid (=D-araboascorbic acid; D-erythorbic acid), D-glucoascorbic acid and all the other analogs were equally acquired by males microfed singly or at mating. The effect of D-optically active analogs on the spermatophore and the possible applications of the analogs in pest control are discussed.
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  • 7
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    Entomologia experimentalis et applicata 40 (1986), S. 61-69 
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Nymphalidae ; Euphydryas chalcedona ; Scrophulariaceae ; Penstemon spp. ; hostplant specificity ; utilization efficiency ; iridoid glycoside ; insect/plant interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Des chenilles de deux populations d'E. chalcedona ont été élevées sur leur propre plante-hôte et sur celle de l'autre population, aux stades avant et après diapause. Les deux populations s'alimentent sur différentes espèces de Penstemon (Scrophulariaceae), et une population—Echo Lake—est monophage sur P. newberry, tandis que l'autre—Chico—utilise d'abord P. breviflorus, mais les chenilles après diapause sont trouvées sur au moins deux autres espèces de plantes. Les taux de croissance et de survie ont été déterminés pour des chenilles avant et après diapause pour les deux populations sur les deux plantes; les efficacités digestives ont été calculées sur les chenilles avant diapause. Les résultats ont montré que les chenilles des deux populations différaient par leur degré de spécialisation digestive sur leur plante hôte normale: les chenilles de Chico ont utilisé aussi bien les deux plantes, tandis que celles d'Echo Lake le faisaient significativement moins bien sur la plante non-hôte, par suite de l'inaptitude à la digérer. Ainsi la population oligophage est alimentairement moins spécialisée et plus capable de se débrouiller avec une plante non-hôte. Après diapause, les chenilles de Chico s'alimentaient significativement mieux sur plante hôte que non-hôte, ce qui était le cas aussi pour la population monophage. Dans l'ensemble, les chenilles de la population monophage semblaient moins capables de se débrouiller dans des conditions défavorables ou moins avantageuses.
    Notes: Abstract Larvae from two populations of Euphydryas chalcedona Doubleday & Hewitson (Nymphalidae) were reared on their own hostplant and that of the other population, in both pre-diapause and post-diapause instars. One population, Chico, uses Penstemon breviflorus Lindl. (Scrophulariaceae), and the other, Echo Lake, uses P. newberryi Gray. Growth rate and survival were determined for pre-diapause and post-diapause larvae from both populations on both plant species; and digestive efficiencies were calculated during the prediapause instars. The results showed that larvae from the two populations differed in their responses to the two plant species. Pre-diapause larvae from Chico performed equally well on both plant species—survival and digestive indices were not significantly different for two Penstemon species. In contrast, pre-diapause larvae from Echo Lake performed significantly worse on the non-hostplant—growth and survival were significantly lower on the non-host, P. breviflorus. In addition, comparison of digestive efficiencies for the two plants showed that larvae from Echo Lake digested P. breviflorus better than P. newberryi, but were significantly less able to convert P. breviflorus to body mass. In the post-diapause instars, larvae from Chico grew faster on the host than on the non-host. Larvae from Echo Lake grew quite slowly on both plant species and significantly more of the Echo Lake larvae returned to diapause instead of completing development.
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  • 8
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    Entomologia experimentalis et applicata 40 (1986), S. 71-79 
    ISSN: 1570-7458
    Keywords: oviposition deterrent ; contact chemoreceptors ; electrophysiology ; tarsi ; ovipositor ; Pieris brassicae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Une technique d'enregistrement apical a été utilisée pour examiner la perception d'une phéromone dissuadant la ponte par les poils récepteurs chimiques de contact des femelles de Pieris brassicae. Les réponses électrophysiologiques des poils gustatifs des tarses en présence de solutions de rinçage d'oeufs présentent une fréquence marquée des potentiels d'action provenant principalement d'une cellule sensorielle. Ceci suggère que les poils gustatifs des tarses des pattes antérieures des femelles possèdent, en plus de cellules répondant aux glucosinolates, des cellules sensorielles sensibles spécialement à la phéromone dissuadant la ponte. Des études morphologiques au microscope à balayage révèlent que l'oviposition de P. brassicae est pourvu de deux groupes de chimiorécepteurs de contact. Des enregistrements électrophysiologiques de ces sensilles révèlent la présence d'au moins trois cellules sensorielles, l'une d'entre elles étant un mécanorécepteur. La stimulation avec la solution de rinçage des oeufs évoque un léger accroissement de la fréquence des potentiels d'action qui ne peut être attribué à une cellule sensorielle particulière. Ceci indique que les poils gustatifs abdominaux peuvent participer d'une certaine façon à la perception de la phéromone dissuadant la ponte. Les réponses aux glucosinolates ne diffèrent pas significativement des stimulations témoin.
    Notes: Abstract Perception of the oviposition deterring pheromone by contact chemoreceptors in female Pieris brassicae was studied employing a tip recording technique. Electrophysiological responses of tarsal taste hairs to eggwash solutions show a marked increase in frequency of spikes originating from only one sensory cell. This suggests that in foretarsal taste hairs females, apart from the glucosinolate cells also possess sense cells specifically sensitive to the oviposition deterring pheromone. Morphological studies by means of the scanning electron microscope revealed that the ovipositor of P. brassicae is provided with two groups of contact chemoreceptors. Electrophysiological recordings from these sensilla indicate the presence of at least three sensory cells, one of them being a mechanoreceptor. Stimulation with eggwash evokes a slight increase in spike frequency which cannot be ascribed to one particular sense cell. This indicates that abdominal taste hairs in some way may participate in the perception of the oviposition deterring pheromone. Responses to glucosinolates do not differ significantly from control stimulations.
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  • 9
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    Entomologia experimentalis et applicata 30 (1981), S. 123-127 
    ISSN: 1570-7458
    Keywords: Sex Pheromones ; Repellent ; Heliothis armigera ; Heliothis zea ; Lepidoptera ; Noctuidae ; cotton bollworm ; corn earworm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'examen en olfactomètre a porté sur les réactions face à d'autres femelles de la même espèce, de femelles vierges ou ayant copulé d'Heliothis armigera Hübner et H. zea Boddie. Le lot comprenait 8 femelles, vierges ou ayant copulé en présence d'une femelle vierge ou ayant copulé. Les 4 combinaisons possibles de femelles vierges et de femelles ayant copulé ont été examinées avec 12 répétitions pour chaque espèce. Un extrait de l'extrémité de l'abdomen de femelles vierges d'une espèce a été présenté aux femelles de l'autre espèce pour examiner les possibilités de réactions interspécifiques aux phéromones. Pour chaque espèce, les réactions interspécifiques de répulsion entre femelles ont été hautement significatives par rapport aux témoins, à l'exception toutefois des réactions de femelle ayant copulé face à des femelles ayant elles aussi copulé. Les répulsions moyennes chez H. armigera et H. zea pour les 8 femelles de chaque expérience ont été: a) vierges en présence d'une vierge: 7,33 et 7,66; b) vierges en présence d'une femelles ayant copulé: 5,76 et 5,58; c) femelles ayant copulé en présence d'une vierge: 4,67 et 4,83. Les différences sont hautement significatives entre chaque paire de moyennes et entre chaque paire et le lot témoin; 3,17; 3,17; 3,42; 4,00 pour H. armigera; 3,17; 3,50; 2,83 et 3,75 pour H. zea. Les femelles vierges des deux espèces, H. armigera et H. zea ont présenté une réaction de répulsion en présence d'un extrait de l'abdomen de l'autre espèce; les répulsions moyennes étant respectivement 5,53 et 5,33 contre 3,83 et 3,58 pour le lot trémoin. On peut en conclure que ces répulsions doivent entraîner une tendance à la répartition uniforme.
    Notes: Abstract An olfactometer was used to determine the effect of pheromones released by females of the bollworms Heliothis armigera (Hübner) and H. zea (Boddie) on females of the same species. Four combinations of virgin and mated females were tested for repellency of one to the other. Evidence is presented that females of the two bollworms were repelled by females of the same species. In addition, extracts of virgin female abdomens of each species repelled virgin females of the other species.
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  • 10
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    Entomologia experimentalis et applicata 41 (1986), S. 139-146 
    ISSN: 1570-7458
    Keywords: oviposition deterrent ; pheromone ; egg washings ; Pieris brassicae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Des expériences de laboratoire ont été réalisées pour déterminer d'influence d'une phéromone dissuasive de la ponte (ODP) sur la distribution des ooplaques et des oeufs de Pieris brassicae. Cette phéromone est connue comme associée aux oeufs. Les papillons ont eu le choix entre des feuilles de chou traitées de différentes façons avec ODP et des feuilles témoins. La présence d'oeufs intacts de la même espèce sur les feuilles a un effet dissuasif modéré sur la ponte. Une solution aqueuse d'ODP, obtenue par lavage des oeufs à l'eau distillée est quelque peu dissuasive. Le lavage des oeufs de P. brassicae au méthanol est apparu comme le plus dissuasif. Une telle solution au méthanol peut être stockée à basse température au moins 3 ans sans perte de son pouvoir. Les applications de l'eau de rinçage sur les faces supérieures ou inférieures des feuilles induisent le même comportement des femelles. L'effet dissuasif augmente avec la concentration de l'eau de rinçage; aux très faibles concentrations, aucune différence significative n'a pu être observée avec les témoins quant au nombre d'ooplaques et d'oeufs. Par ailleurs, même aux très hautes concentrations du méthanol, l'eau de rinçage n'inhibe pas totalement la ponte sur les feuilles de chou. En lavant 7 fois consécutives les oeufs de P. brassicae dans le méthanol, on obtient une série de solutions de phéromones, dont chacune possède un fort pouvoir dissuasif. Bien que l'effet dissuasif diminue lentement avec des rinçages successifs, la pulvérisation du septième rinçage sur les feuilles de chou réduit encore à moins du quart le nombre total d'ooplaques et des oeufs pondus sur la feuille traitée.
    Notes: Abstract A laboratory study was conducted in order to determine the influence of the oviposition deterring pheromone (ODP) upon the distribution of eggbatches and eggs by Pieris brassicae females. This pheromone is known to be associated with eggs. Butterflies were offered a choice between cabbage leaves treated in various ways with the ODP and control leaves. The presence of intact conspecific eggs on the treated leaf appeared to have a moderate deterrent effect upon oviposition. An aqueous solution of the ODP, obtained by washing eggs in distilled water was found to have a somewhat higher deterrent effect. Most effective in deterrence of oviposition, however, appeared to be a washing of P. brassicae eggs in methanol. Such a methanol solution can be stored at low temperatures for at least three years without loosing activity. Application of eggwash to either the upper or lower surface of the leaf does not make any difference to females. Percentage deterrence was found to increase with the concentration of eggwash. At very low concentrations no significant difference could be observed anymore in the numbers of eggbatches and eggs laid on control and treated leaf. On the other hand, even very high concentrations of methanol eggwash do not fully protect cabbage leaves against oviposition. Washing P. brassicae eggs seven times consecutively in methanol, a series of pheromone solutions is obtained, all of them were found to possess a high deterrent activity. Although percentage deterrence slowly decreases in subsequent washings, the seventh eggwash sprayed onto cabbage leaves still resulted in less than one quarter of the total number of eggbatches and eggs being laid on the treated leaf.
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  • 11
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    Entomologia experimentalis et applicata 40 (1986), S. 297-301 
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Gracillariidae ; Cameraria sp. ; leaf miners ; mortality ; oaks ; parasitism ; tannins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Des extraits riches en tanins ont été étalés sur des feuilles de Quercus emoryi contenant des chenilles de ler stade de Cameraria sp., pour contrôler l'influence du tanin dans l'accroissement du parasitisme sans modifier la teneur en protéines ni provoquer de dommages physiques. Les mineuses de feuilles traitées avaient des taux plus élevés de parasitisme par les hyménoptères, mais une diminution des autres causes de mortalité. Les effets négatifs et positifs des tanins se sont équilibrés, puisque la survie des mineuses était la même pour les feuilles traitées que pour les témoins. Les extraits riches en tanin ont agi sur le développement larvaire puisque le poids des chrysalides des mineuses de feuilles traitées était plus faible. Les tanins peuvent ainsi avoir des effets nuisibles et bénéfiques sur ces insectes phytophages.
    Notes: Abstract Tannin-rich extracts were painted on leaves of Q. emoryi (Fagaceae) that contained first instar larvae of a leaf miner, Cameraria sp. (Lepidoptera: Gracillariidae, Agrifoliella group), to test the role of tannins in increasing parasitism, while controlling for changes in protein content and physical damage. Miners from leaves that were treated with tannins had higher rates of parasitism by hymenopterans, but decreased mortality from other causes. The negative and positive effects of tannins balanced since overall larval survivorship was equivalent for leaf miners on tannin-enriched and control leaves. The tannin-rich extract also affected larval development because pupal weight was decreased for leaf miners in treated leaves. Tannins can have both adverse and beneficial effects on these phytophagous insects.
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  • 12
    ISSN: 1570-7458
    Keywords: (Z)-11-hexadecenyl acetate ; (Z)-11-hexadecenal ; (Z)-11-hexadecenol ; (Z)-9-tetradecenyl acetate ; hexadecanyl acetate ; Mamestra suasa ; Lepidoptera ; Noctuidae ; sex pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé On a trouvé l'acetoxy-1 hexadécene-11 Z (Z-11-16:Ac), le hexadécene-11 Z al-1 (Z-11-16:Ald), le hexadécene-11 Z ol-1 (Z-11-16:OH) et l'acetoxy-1 hexadécene (16:Ac) dans des extraits de glandes phéromona les des femelles deMamestra suasa. La proportion relative des composés était 100/2/10/5. Tous les quatre composés ont été présents aussi dans les collections d'émanations des femelles en stade d'appel, dans la proportion un peu différente de 100/7/5/5. On n'a détecté aucune trace des tétradécenes al-1 ou d'acetoxy-1 tétradécenes. On a démontré la présence de hexadécenoate-1 methyl, hexadécene-9 Z oate-1 methyl et héxadécene-11 Z oate-1 methyl dans des extraits des glandes, par la méthode de ‘base methanolysis’. On n'a trouvé pas des tétradéceneoates methyl. En éléctroantennographie, Z-11-16:Ac a donné les meilleurs réponses, suivis par l'acetoxy-1 tétradécene-9 Z (Z-9-14:Ac), Z-11-16:Ald et Z-11-16:OH. Dans des études de ‘single sensillum’ les cellules à amplitude grande ont répondu à la stimulation avec de Z-11-16:Ac, cependant les cellules à amplitude petite ont répondu à la stimulation avec des deux composés Z-9-14:Ac et Z-11-16:OH. On a trouvé des cellules sensitives à Z-11-16:Ald dans 1 entre 60 sensilla étudiés. À la chambre de vol, le dose de 0.1 μg d'un mélange de 10:1 de Z-11-16:Ac/Z-11-16:Ald a provoqué les mêmes réponses et à l'intensité pareille comme 3 glandes phéromonales isolées des femelles. En piégeages sur le champs des males en quantité importante ont été capturé par un mélange de 10:1 de Z-11-16:Ac/Z-11-15:Ald en Bulgarie et Hongrie. L'addition de 16:Ac au mélange binaire n'avait aucun effet, cependant l'addition de plus de 1% de Z-11-16:OH ou 0.1% de Z-9-14:Ac a sérieusement diminué les captûres. En comparant des proportions différentes du mélange de l'acetoxy/aldéhyde dans des doses différentes, on a observé les meilleurs captûres avec de la proportion 10:1 et à la dose la plus haute (1 000 μg).
    Notes: Abstract (Z)-11-hexadecenyl acetate (Z-11-16:Ac), (Z)-11-hexadecenal (Z-11-16:Ald), (Z)-11-hexadecenol (Z-11-16:OH) and hexadecanyl acetate (16:Ac) were found in pheromone gland extracts of femaleMamestra suasa (Den. et Schiff.) in the relative amounts 100/2/10/5. All four compounds were also present in collections of aiiborne volatiles from calling females in a 100/7/5/5 ratio. No traces of 14 carbon aldehydes or acetates were detected. In gland extracts the presence of methyl hexadecanoate methyl (Z)-9-hexadecenoate and methyl (Z)-11-hexadecenoate was demonstrated by base methanolysis. No methyl tetradecenoates were detected. In EAG tests Z-11-16:Ac gave the best responses, followed by (Z)-9-tetradecenyl acetate (Z-9-14:Ac) Z-11-16:Ald and Z-11-16:OH. In single sensillum recordings large spike amplitude cells in sensilla responded to Z-11-16:Ac, while small spike amplitude cells to both Z-11-16:OH and Z-9-14:Ac. Cells responding to Z-11-16:Ald were found in one out of 60 sensilla tested. In wind tunnel tests 0.1 μg of a 10:1 blend of Z-11-16:Ac/Z-11-16:Ald evoked the same responses and at a similar intensity as 3 isolated female pheromone glands did. In field tests a 10:1 blend of Z-11-16:Ac/Z-11-16:Ald caught significant numbers of males in both Bulgaria and Hungary. The addition of 16:Ac to the binary blend did not have any effect, while more than 1% of Z-11-16:OH or 0.1% of Z-9-14:Ac dramatically decreased captures. In comparing different ratios of the, acetate/aldehyde blend at different dose levels, best catches were recorded at the 10:1 ratio and at the highest (1000 μg) dose level.
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  • 13
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    Entomologia experimentalis et applicata 42 (1986), S. 45-55 
    ISSN: 1570-7458
    Keywords: Yponomeuta padellus ; Lepidoptera ; Yponomeutidae ; courtship ; behaviour ; quantitative analysis ; pheromone communication ; wind tunnel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les séquences du comportement depuis l'attraction du mâle jusqu'à la copulation ont été décrites et analysées chez Y. padellus (L.). Les mâles réagissent à une femelle émenttant une phéromone par un hattement d'ailes suivi d'un vol en zigzag contre le vent. Les mâles atterriront près des femelles et marcheront avec leurs ailes en battement. Les antennes tambourinent le substrat et les pinceaux abdominaux sont déplyés. Lorsqu'il est au contact de la femelle, le mâle tambourine avec ses antennes les ailes, la tête et les antennes. Généralement suit une tentative d'étreinte. Les chances de succès sont les plus élevées quand les premiers contacts du mâle sont avec la partie postérieure de la femelle plutôt qu'avec la tête. Les femelles refusent les mâles en s'écartant ou en battant des ailes ou les deux à la fois. Il semble qu'en plus des stimuli cliniques, des stimuli mécaniques fournis par les écailles sont nécessaires pour que les mâles localisent la femelle et s'orientent pour une tentative d'étreinte.
    Notes: Abstract The sequence of behavioural steps from male attraction until copulation is described and analyzed for Yponomeuta padellus L. (Yponomeutidae, Lepidoptera). Males respond to a pheromone emitting female with wing fanning followed by upwind zigzagging flight. Males will land near the females and then walk while wing fanning. The antennae drum onto the substratum and the abdominal-hair pencils are displayed. When in contact with a female the male drums with his antennae onto wings, head and antennae. Mostly a clasping attempt follows. A male has a higher chance to be accepted when he firt encounters the rear of a female instead of the head. Females refuse males by walking away or wing fanning or a combination of both.
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  • 14
    ISSN: 1570-7458
    Keywords: Pyrale du maïs ; Ostrinia nubilalis ; Lepidoptera ; Pyralidae ; attraction plante-hôte ; peuplement-refuge ; piégeage lumineux ; lâcher insectes marqués ; coloration interne ; protandrie ; phéromone ; European corn borer ; Ostrinia nubilalis ; Lepidoptera ; Pyralidae ; host plant attraction ; refuge plant association ; light trapping ; internal coloration technique ; release of marked insects ; protandry ; pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary The authors used their light trapping method associated with the release of marked insects. The experiment took place in an agrosystem in Charente (North of Aquitaine) between June 5 and July 15, 1983. The site included four adjoining plots from 1 to 3 ha each: one plot of wheat (previous crop: maize); two plots of maize (previous crop: barley) and a plot of barley (previous crop: wheat). To demonstrate the possible emergence of moths from hibernating larvae, the marked insects (510 ♂ ♂ and 467 ♀ ♀) were released in the plot of wheat equidistant from the four light traps placed in each plot. The captures of wild insects (1625 ♂ ♂, 786 ♀ ♀) showed an 8-days protandry and a considerable predominance of males in June/July 1983. This seems to have been caused by their being more mobile than the females. Despite the first males were distributed at random in the different plots, the remainder moved towards the maize. Such behaviour could be associated with that of the females; the latter move very early into the maize and probably lure the males by the release of sex-pheromone. The captures of marked insects (12% of the released population) confirmed the observations obtained with wild insects. The ecological and agronomical consequences of these migrations are discussed. It is suggested that there exists a pattern of two types of movements of European corn borer moths: emergence migration and diurnal migration.
    Notes: Résumé Au cours de l'été 1983 dans un agrosystème de Charente (Nord du Bassin Aquitain), les auteurs ont utilisé la technique du piégeage lumineux associée à des lâchers d'insectes marqués. Afin de mimer les émergences possibles des papillons à partir de chenilles hivernantes, les insectes colorés étainent lâchés dans une parcelle de blé (Triticum) (à antécédent cultural maïs), à égale distance de 4 pièges disposés dans 4 parcelles adjacentes. Les captures d'insectes colorés montrent clairement que les femelles quittent le blé et se dirigent vers le maïs, confirmant par là le rôle de l'antécédent cultural sur les infestations annuelles. Les captures d'insectes sauvages indiquent à cette époque une prédominance des mâles due probablement à la protandrie d'environ 8 jours chez l'espèce et à une mobilité naturelle supérieure à celle des femelles. Les implications écologiques et agronomiques de ces déplacements sont discutées et un schéma d'explication des différents types de mouvements des papillons de pyrale: déplacements d'émergence et déplacements quotidiens alternatifs, est proposé.
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  • 15
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    Entomologia experimentalis et applicata 41 (1986), S. 311-313 
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Sesiidae ; clearwing moths ; Synanthedon ; sex attractants ; pheromone synergism ; pheromone receptors ; octadecadienyl acetates
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  • 16
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    Entomologia experimentalis et applicata 42 (1986), S. 9-17 
    ISSN: 1570-7458
    Keywords: Lepidoptera ; behaviour ; formamidines ; pyrethroids ; prolegs ; crop protection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Des doses sublétales de chlordiméforme (CDM), insecticide formamidine, injectées dans des chenilles de Saturniidae, provoquent divers effets comportementaux. Ceux-ci comprennent l'arrêt de l'alimentation, l'augmentation du taux de locomotion, une hyperactivité générale de la tête et de ses appendices, et des mouvements désordonnés des pattes; Une conséquence de ce dernier effet est une réduction de l'étreinte réelle du substrat par les chenilles. Il a été étudié en détail sur les chenilles de cinquième stade de Philosamia cynthia ricini. Pour toute une gamme de doses, les effets les plus nets ont été observés sur les fausses pattes. Ils on été évalués par une série d'observations continues de chenilles placées sur une baquette de bois, permettant d'observer clairement le comportement d'étreinte. Les effet étaient réversibles. Des comparaisons ont été réalisées en utilisant la cyperméthrine, insecticide pyréthroïde. Les chenilles traitées ont présenté un certain nombre de comportements d'irritation, mais aucune réponse spécifique d'étreinte n'a été notée. La discussion de ces résultats examine les possibilités d'action de CDM dans la nature. Compte tenu de l'importance jouée par les fausses pattes dans le maintien sur la plante, on peut envisager que la suppression de l'étreinte peut entraîner une protection efficace des cultures.
    Notes: Abstract General observations of the behavioural effects of sublethal doses of the fomamidine pesticide chlordimeform (CDM) on saturniid larvae led to a detailed investigation of the disruption of proleg clasping caused by CDM in Philosamia cynthia ricini Hutt. (Castor Silk Moth) larvae. Subjects received doses ranging from 5.09×10−3 to 19.3 μg/g body weight. Doses above 0.15 μg/g caused uncoordinated movements of the legs which led to reductions in the effective grip on the substrate. Greatest interference was recorded in the prolegs. The effects were reversible. Significance of these results is discussed in relation to their possible role in crop protection mechanisms. In investigating the degree of specificity of the observed effects as a mode of insecticidal action, larvae were treated with a pyrethroid compound, cypermethrin. Doses ranging from 0.002 to 0.842 μg/g resulted in reduction in the times the larvae spent firmly attached to the substrate. It is concluded that this is a consequence of an overall excitation caused by the insecticide, displayed as a number of described irritant behaviours, rather than any specific disruption of proleg action.
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  • 17
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    Entomologia experimentalis et applicata 42 (1986), S. 109-117 
    ISSN: 1570-7458
    Keywords: cabbage looper ; Trichoplusia ni ; Glycine max ; soybeans ; trichomes ; plant resistance ; Noctuidae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'influence de la densité et de la longueur des trichomes de Glycine max sur la résistance à Trichoplusia ni a été évaluée suivant la position des feuilles sur une lignée résistante (‘PI 227687’) et sur un cultivar sensible (‘Davis’). Les feuilles apicales (juvéniles) tant de PI 227687 que de Davis, recouvertes de trichomes denses, résistèrent mieux à l'alimentation larvaire et à la survie de T. ni. Quand ces trichomes étaient éliminés, ces feuilles ne résistaient pas plus à l'alimentation des larves de T. ni que les feuilles non rasées TL3 et TL5 de PI 227687 ou toutes les autres feuilles de Davis. Les tests avec des extraits dans l'acétate d'éthyle et l'hexane de feuilles provenant de différentes positions de PI 227687 et Davis, destinés à mettre en évidence des phagodissuadants, ont montré que la résistance observée chez les feuilles apicales de Davis était attribuable aux trichomes (c'est-à-dire à un caractère morphologique), tandis que chez les mêmes feuilles de PI 227687 elle impliquait à la fois des trichomes (morphologie) et des substances chimiques, mais avec une prédominance de l'influence des trichomes.
    Notes: Abstract Role of leaf trichome density and length in Glycine max (L.) Merr. resistance to Trichoplusia ni (Hübner) was evaluated at different leaf positions on a relatively resistant soybean, plant introduction (PI) 227687, and a relatively susceptible cultivar, ‘Davis’. The uppermost (juvenile) leaf within both PI 227687 and ‘Davis’ plants, which is densely covered with trichomes, was most resistant to T. ni larval feeding and survival. When these trichomes were shaven off, such leaves became as susceptible to T. ni larval feeding as unshaven TL3 and TL5 leaves (PI 227687) or all other unshaven leaves (‘Davis’). Bioassays for antifeedants in ethyl acetate and hexane extractables from leaves at the different positions on PI 227687 and ‘Davis’ plants showed that the resistance observed in the uppermost ‘Davis’ leaf is attributable to trichomes (i.e., a morphological factor); whereas, in the uppermost PI 227687 leaf it involves both morphological (trichomes) and chemical factors, but the trichome parameter is dominant.
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  • 18
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    Entomologia experimentalis et applicata 42 (1986), S. 301-302 
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Philosamia ricini ; Eri silk worm ; cellobiose ; cellobiase
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  • 19
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    Calcified tissue international 33 (1981), S. 409-416 
    ISSN: 1432-0827
    Keywords: Bone Cells ; Cyclic AMP ; Calcium ; Parathyroid hormone ; Prostaglandin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary We have examined the influence of extracellular Ca2+ on cyclic AMP metabolism in an osteoblast-enriched population of bone cells isolated from the calvaria of rat fetuses. The cyclic AMP1 response to stimulators of cyclic AMP formation (PTH and PGE2), but not basal cyclic AMP levels, increased progressively as the extracellular Ca2+ concentration was raised from 0.2 to 4.0 mM. The response to changes in extracellular Ca2+ were rapid (within 3.5 min), and the level of responsivity that characterized each Ca2+ concentration persisted for at least 6 h when the Ca2+ concentration was kept constant. The effect of Ca2+ spanned the entire time course of PTH action, was not accompanied by altered excretion of cyclic AMP from the cells, and was evident at low as well as at high hormone concentrations. Ca2+ augmented the action of PTH in the presence as well as in the absence of cyclic AMP phosphodiesterase inhibitors, and failed to decrease cyclic AMP phosphodiesterase activity in the short term. Mn2+ and, to a smaller degree, Ba2+ substituted for Ca2+ in promoting the cyclic AMP response to PTH. Verapamil, an inhibitor of Ca2+ penetration, blunted the Ca2+-mediated increments in the cyclic AMP response, and the divalent cation ionophore A23187 enhanced these increments. These results indicate that Ca2+ and other cations are positive effectors of the stimulated cyclic AMP response in isolated bone cells. Accumulation into an as yet unknown cellular compartment may be required for the cation effect. The data are most consistent with enhancement of adenylate cyclase reactivity as the mode of cation action.
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  • 20
    ISSN: 1420-9071
    Keywords: Acid fluoride ; aldehyde ; mating disruption ; pheromone analogs ; Heliothis ; Lepidoptera ; fluorination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Substitution of fluorine for the aldehydic hydrogen provides behaviorally active, chemically reactive pheromone mimics. In male moths of the tobacco budwormHeliothis virescens, Z9–14:Acf and Z11–16:Acf cause hyperactivity and irreversible extension of the genitalia in over 80% of treated moths. In addition, a combination of the two components leads to 10–50% of the pairs involving one treated partner becoming locked in copula.
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  • 21
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    Cellular and molecular life sciences 42 (1986), S. 448-450 
    ISSN: 1420-9071
    Keywords: Lepidoptera ; Acrolepiopsis assectella ; host plant ; larval diet ; ovarian production ; stimulation ; insemination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Females ofAcrolepiopsis assectella, reared on a semi-synthetic diet and laying on artificial substrates, do not respond to external stimuli by increasing ovarian production. When returned to the natural host (Allium porrum) for only one generation, ovarian production again rises and reaches the same level as in wild females, but its variability is strongly reduced. We conclude that selection under artificial conditions eliminates individuals which strictly depend on host plants for stimulation of larval nutrition and of reproduction.
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  • 22
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    Cellular and molecular life sciences 42 (1986), S. 1059-1061 
    ISSN: 1420-9071
    Keywords: Pheromone ; Lepidoptera ; Agrotis segetum ; biosynthesis ; palmitic acid ; capillary gas chromatography ; deuterium ; polyethylene glycol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary (16-D3)palmitic acid was applied topically to pheromone glands of the turnip moth. After incubation omega labeled analogues of fatty acid methyl esters and acetates were identified by gas chromatography with flame ionization detection and selected ion monitoring. The pheromone components (Z)-5-decenyl, (Z)-7-dodecenyl- and (Z)-9-tetradecenyl acetate were all biosynthesized from palmitic acid along a common pathway.
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  • 23
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    Calcified tissue international 33 (1981), S. 365-368 
    ISSN: 1432-0827
    Keywords: Absorptiometry ; Osteoporosis ; Spinal bone ; Bone mineral content ; Neutron activation ; Calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Dual-photon absorptiometry using153Gd (44 and 100 keV) was used to measure the bone mineral content (BMC) of the trunk and of the total body (TBBM) in 7 volunteers with no overt bone disease. These values were compared to those obtained with partial-body neutron activation of calcium (trunk Ca). The trunk Ca seemed to represent best a 60 × 30 cm area; the correlation coefficient with the corresponding BMC in that area was 0.97 (SEE ⋍ 7%). Trunk Ca was also highly correlated with TBBM (r=0.96; SEE=8%) and with radius BMC (r=0.92; SEE=11%), but the correlations with the BMC of smaller subareas of the trunk were lower (r⋍0.9; SEE ∼ 12%). The BMC of the lumbar spine was only moderately correlated with trunk Ca, radius BMC and TBBM (r ∼ 0.82; SEE ∼ 18%), and only slightly more associated with trunk BMC (r ∼ 0.88; SEE ∼ 14%). The BMC of the combined lumbar-thoracic spine showed higher correlations with trunk Ca, radius BMC and TBBM (r ∼ 0.87; SEE ∼ 13%), and trunk BMC (r=0.93; SEE ∼ 10%). An accurate and sensitive measure of spinal status requires a direct measurement of that area.
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  • 24
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    Calcified tissue international 33 (1981), S. 477-484 
    ISSN: 1432-0827
    Keywords: 1,25-(OH)2-D ; Calcium ; Phosphorus ; Sex ; Age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Dietary phosphate deprivation in women, but not men, is accompanied by a fall in plasma PO4 and a rise in plasma 1,25-(OH)2-vitamin D concentrations. In contrast, young male rats exhibit a fall in plasma PO4 and a rise in plasma 1,25-(OH)2-D concentrations in response to PO4 deprivation. To evaluate whether age and sex influence basal plasma 1,25-(OH)2-D levels and their regulation by PO4 deprivation, plasma 1,25-(OH)2-D, PO4, and Ca levels were measured in male and female rats ranging in age from 6 weeks to 6 months while they were eating normal or low PO4 diets for 1 to 16 days. Similar observations were also made in 6-week-old castrated male and female rats, males replaced with testosterone, and females replaced with estradiol. Basal plasma 1,25-(OH)2-D levels were higher in 6-week-old males (228±76 pmol/l) than in 6-week-old females (148±62 pmol/l;P〈0.01) and declined by age 11 weeks to stable levels averaging about 100 pmol/l without sex difference. Dietary PO4 deprivation resulted in a three-to fourfold increase in plasma 1,25-(OH)2-D concentrations regardless of age and sex, accompanied by a correlated rise in serum Ca concentrations. Castration of 6-week-old males and females eliminated the sex difference in basal plasma 1,25-(OH)2-D levels and appeared to enhance the elevation of plasma 1,25-(OH)2-D concentrations in response to PO4 deprivation in females. Although gonadal hormones may modify basal plasma 1,25-(OH)2-D levels, they are not required for the augmentation of plasma 1,25-(OH)2-D levels in response to PO4 deprivation.
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  • 25
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    Calcified tissue international 33 (1981), S. 575-581 
    ISSN: 1432-0827
    Keywords: Osteoporosis ; Vitamin D ; Bone mineral ; Estrogen ; Aging ; Thiazide ; Fluoride ; Calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Bone mineral content (BMC) was measured by125I photon absorptiometry every 3 months in 264 normal females (45–54 years) over a 2-year period together with serum samples for calcium, phosphate, magnesium, creatinine, alkaline phosphatases, potassium, and protein. A 48-h urinary calcium and creatinine measurement was obtained. The subjects were divided into 7 treatment groups and 3 placebo groups. Five of the treatments (thiazide, vitamin D3, fluoride + vitamin D3, fluoride, and 1αD3) were ineffective at the doses used; the annual loss of compact bone was 1.5–2.2% (-X=1.8%), similar to the loss seen with placebos. Estrogen and estrogen + thiazide, in contrast, produced a 1.34% annual increase of BMC. The subjects were divided into groups with low, medium, and high initial BMC. Also, individual regressions for bone change were calculated and the subjects were divided into groups of responders, maintainers, and losers (annual change of 〉0%, 0 to −1%, and 〉−1%, respectively). The initial BMC status did not consistently affect bone or biochemical responses to the therapeutic agents. Estrogen was effective even in subjects with high BMC, whereas the other agents did not inhibit bone loss even in subjects with low initial BMC. Virtually all subjects responded to estrogen positively; in contrast we could not identify a subset of “responders” with any of the other treatments. Time since menopause appeared to influence the bone changes, although it was not a significant effect given the sample size. Bone loss in groups not treated with estrogens was 2%/year at 20 months after menopause with a decline to 1.3%/year at 45 months post-menopause. There was no apparent decline in the bone response to estrogen during the first 4 years after menopause, and in fact bone response tended to increase with time.
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  • 26
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    Calcified tissue international 33 (1981), S. 485-488 
    ISSN: 1432-0827
    Keywords: 1,25-(OH)2-D ; Hypophysectomy ; Calcium ; Phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The mechanism by which dietary phosphate deprivation elevates plasma 1,25-(OH)2-D levels is not known. To evaluate the role of the pituitary in regulating plasma 1,25-(OH)2-D concentrations, the responses of plasma 1,25-(OH)2-D to dietary phosphate deprivation and, separately, to dietary calcium deprivation were evaluated in intact and hypophysectomized male rats. Among intact and hypophysectomized rats eating normal diets, plasma 1,25-(OH)2-D levels averaged 228±76 and 148±62 pmol/1, respectively (P〈0.01). During dietary phosphate deprivation, plasma 1,25-(OH)2-D levels rose to 1160±260 in intact rats and fell to 90±26 pmol/l in hypophysectomized rats (P〈0.001). By contrast, during dietary calcium deprivation, plasma 1,25-(OH)2-D levels rose in both intact and hypophysectomized animals to 856±107 and 742±279 pmol/l, respectively (NS). In response to dietary phosphate deprivation, serum calcium concentrations rose as 1,25-(OH)2-D concentrations rose in intact rats but remained at control levels in hypophysectomized rats. These results support the hypothesis that a pituitary hormone acting either directly or indirectly on the kidney mediates the increase in plasma 1,25-(OH)2-D during dietary phosphate deprivation. The hypercalcemia that occurs in rats during dietary phosphate deprivation appears to depend on the elevation of plasma 1,25-(OH)2-D.
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  • 27
    ISSN: 1432-2048
    Keywords: Calcium ; Cation exchange ; Lycopersicon esculentum ; Rhythm ; diurnal Transport (calcium) ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tomato (Lycopersicon esculentum Mill. cv. Moneymaker) and maize (Zea mays L. cv. spec.) plants were supplied with 45Ca-labeled nutrient solutions for a period of 8 or 16 h in the dark, in the light, or in a light-dark régime. Plant parts were analyzed for 45Ca content. The partitioning of 45Ca between mature leaves and meristems was shown to be affected by the presence of light. The transport of 45Ca to meristems was higher in a dark period than in a comparable light period. Experiments with excised tomato shoots yielded similar distribution patterns of 45Ca over leaves and meristems, thus excluding root pressure as the main driving force for the enhanced import of 45Ca into the meristems in the dark. Results are discussed in terms of cation-exchange transport and competition between the various calcium sinks.
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  • 28
    ISSN: 1432-2048
    Keywords: Bryophyta ; Bud formation (moss) ; Calcium ; Chlorotetracycline ; Cytokinin ; Funaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the changes in membrane-associated calcium that occur during cytokinin induced bud formation in Funaria hygrometrica Hedw. using the fluorescent Ca2+-chelate probe chlorotetracycline (CTC). In the target caulonema cells a localization of CTC fluorescent material becomes evident at the presumptive bud site 12 h after cytokinin treatment. By the time of the initial asymmetric division this region is four times as fluorescent as the entire caulonema cell. Bright CTC fluorescence remains localized in the dividing cells of the bud. To relate the changes in CTC fluorescence to changes in Ca2+ as opposed to membrane-density changes we employed the general membrane marker N-phenyl-1-naphthylamine (NPN). NPN fluorescence increases only 1.5 times in the initial bud cell. We conclude that the relative amount of Ca2+ per quantity of membrane increases in this localized area and is maintained throughout bud formation. We suggest that these increases in membrane-associated Ca2+ indicate a localized rise in intracellular free Ca2+ concentration brought about by cytokinin action.
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  • 29
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    Planta 152 (1981), S. 450-456 
    ISSN: 1432-2048
    Keywords: Calcium ; Cell elongation ; Gibberellin ; Lactuca ; pH and growth
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    Topics: Biology
    Notes: Abstract The relationship between calcium ions and gibberellic acid (GA3)-induced growth in the excised hypocotyl of lettuce (Lactuca sativa L.) was investigated. The short-term kinetics of growth responses were measured using a linear displacement transducer. Test solutions were added either as drops to the filter paper on which the hypocotyl stood (“non-flow-past”) or by switching solution flowing past the base of hypocotyl (“flow-past”), resulting in differences in growth behavior. Drops of CaCl2 added at a high concentration (10 mM) inhibited growth within a few minutes. This inhibition was reversed by ethylenediaminetetraacetic acid (EDTA). Drops of EDTA or ethyleneglycol-bis(2-aminoethylether)-tetraacetic acid caused a rapid increase in growth rate. Growth induced by EDTA was not further promoted by GA3. A continuous H2O flow resulted in growth rates comparable to those in response to GA3. Addition of CaCl2 to the flow-past medium inhibited growth and this inhibition was reversed by a decrease in CaCl2 concentration. The growth rate was found to be a function of CaCl2 concentration. When a constant CaCl2 concentration was maintained by the flow-past medium, a shift in pH from 5.5 to 4.25 had no obvious effect on hypocotyl elongation. Gibberellic acid was found to reverse the inhibitory effect of CaCl2, causing an increase in growth rate similar to that found previously when GA3 was added to hypocotyls grown in H2O under non-flow-past conditions. We propose that gibberellin controls extension growth in lettuce hypocotyl sections by regulating the uptake of Ca2+ by the hypocotyl cells.
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  • 30
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    Oecologia 70 (1986), S. 205-213 
    ISSN: 1432-1939
    Keywords: Parnassius ; Lepidoptera ; Radiation ; Temperature ; Melanism ; Thermoregulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The adaptive significance of alpine melanism, the tendancy for insects to become darker with increased elevation and latitude, was investigated using the butterfly Parnassius phoebus. The effects on temperature dependent activity of five components of overall wing melanism, as well as size, were examined. The components of wing melanism examined were the transparency of the basal hindwing and distal fore-wing areas, the width of the black patch in the basal hind-wing area and the proportion of black to white scales in that area, and the proportion of the distal fore-wing covered by predominantly black scaling. The body temperature of dead specimens was correlated with air temperature, solar radiation, the width of the black patch at the base of the wings, and the proportion of black to white scales at the base of the wings. The minimum air temperatures and solar radiation levels required for initiation of flight did not vary with wing melanism of P. phoebus, in contrast to the results found for Colias butterflies by Roland (1982). However, under environmental conditions suitable for flight initiation, males with a higher proportion of black to white scales in the basal area of the hind-wing, and wider basal black patches, spent a greater proportion of time in flight at low air temperatures and low insolation. Increased basal wing melanism was also associated with increased movement of males within a population. In contrast, melanism in the distal area of the wings has no effect on activities which are dependant on body temperature. The amount of time spent feeding did not vary with differences in wing melanism. I suggest that in dorsal basking, slow-flying butterflies (Parnassius) basal wing color affects body temperature primarily during flight (rather than while basking), such that butterflies with darker wing bases cool down less rapidly because they absorb more solar radiation during flight.
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  • 31
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    Archives of microbiology 144 (1986), S. 393-397 
    ISSN: 1432-072X
    Keywords: Chlamydomonas reinhardtii ; Phototaxis ; Motility ; Flagellates ; Flagellar movement ; Calcium ; Calcium channel blockers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of the four calcium channel blockers flunarizine, verapamil, diltiazem and nimodipine on motility and phototaxis of Chlamydomonas reinhardtio have been tested with a fully automated and computerized population system. Flunarizine inhibits motility transiently by causing the detachement of the flagella which, however, are regenerated during some hours. Phototaxis is inhibited to the same extent, but this is simply the result of the decreased motility and, hence, a non-specific effect. Verapamil causes also a detachement of the flagella with following regeneration, but in addition motility and phototaxis are inhibited by this drug to different extents, indicating the involvement of calcium channels in both processes. Diltiazem and nimodipine inhibit phototaxis without impairing motility, indicating that both processes are regulated in different ways. If diltiazem and nimodipine are applied simultaneously, no additive inhibitory effect can be observed. However, the combination of both blockers with verapamil causes and additive inhibitory effect as if verapamil is applied alone. By increasing the external calcium concentration from 10-4 M to 10-3 M the optimum of positive phototaxis is shifted to higher fluence rates. This shifting occurs also in the presence of channel blockers, but the strength of the positive reaction is influenced. These results point to the involvement of calcium channels in both phototaxis and motility, but simultaneously demonstrate the different sensitivity of the two processes to these drugs.
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  • 32
    ISSN: 1573-1561
    Keywords: Picea pungens ; Picea glauca ; feeding deterrent ; spruce budworm ; Choristoneura fumiferana (Clem.) ; Lepidoptera ; Tortricidae ; pungenin ; protection of OH groups ; glucoside ; phenolic glucoside
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Pungenin was synthesized from 3,4-dihydroxyacetophenone by a short sequence involving manipulation of protecting groups on the 3 and 4 hydroxyl functions. Bioassays indicated that the glucoside is a modest feeding deterrent for sixth-instar spruce budworm larvae, but it does not appear to retard the development of small larvae or lead to increased mortality.
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  • 33
    ISSN: 1573-1561
    Keywords: Datana ministra ; Lepidoptera ; Notodontidae ; exocrine prothoracic gland ; ultrastructure ; dispersal pheromone ; dodecanol ; dodecyl acetate ; dodecyl formate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Datana ministra larvae possess a gland situated in the ventral anterior portion of the prothorax. The gland consists of a single sac and is only charged with secretion in the last instar. Its orifice, a simple slit, opens into a transverse invagination of the integument in the cervical margin of the prosternite. The components of the gland have been identified by GC and GCMS and have been shown to be dodecanol, dodecyl acetate, and dodecyl formate. The morphology of the gland and the chemistry of the secretion are discussed in relation to other notodontid larvae which have been investigated. It is conjectured that the secretion is not defensive in nature but may function as a dispersal pheromone.
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  • 34
    ISSN: 1573-1561
    Keywords: Argyrotaenia velutinana ; Lepidoptera ; Tortricidae ; red-banded leafroller ; biosynthesis ; sex pheromone ; mass-labeling ; deuterium labeling ; carbon-13 labeling
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A technique for mass-labeling was developed to study sex pheromone biosynthesis in the red-banded leafroller moth,Argyrotaenia velutinana. With this technique, the pheromone components and all fatty acyl groups in the pheromone gland were analyzed for incorporation of label in the same analytic ran with gas chromatography-mass spectrometry, using chemical ionization and selected ion monitoring (GC-SIM-CI-MS). Sex pheromone glands were incubated with fatty acids or triacylglycerols labeled with at least three deuterium atoms or carbon-13 atoms. The results of these incubations support an interpretation in which hexadecanoate is chain shortened to tetradecanoate, which is desaturated to produce (E)- and (Z)-11-tetradecenoate precursors for the sex pheromone components (E)- and (Z)-11-tetradecen-1-yl acetate. Labeled (E)- and (Z)-11-tetradecenoyl groups in synthetic triacylglycerols were not incorporated into the sex pheromone components, perhaps indicating that this lipid class is not a donor of the immediate fatty acyl precursors in sex pheromone biosynthesis.
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  • 35
    ISSN: 1573-1561
    Keywords: Danaus plexippus ; monarch butterflies ; Lepidoptera ; Danaidae ; Asclepiadaceae ; milkweeds ; Asclepias syriaca ; Asclepias speciosa ; ecological chemistry ; chemical defense ; cardenolides ; aspecioside ; desglucosyrioside ; syriobioside ; overwintering
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The majority (85%) of 394 monarch butterflies sampled from overwintering sites in Mexico contain the same epoxy cardenolide glycosides, including most conspicuously a novel polar glycoside with a single genin-sugar bridge (aspecioside), as occur in the milkweedsAsclepias speciosa andA. syriaca. This cardenolide commonality was established by isolating aspecioside and syriobioside from the wings of overwintering monarchs and the two plant species, and comparing Chromatographie and NMR spectrometric characteristics of the isolates. When combined with the migratory pattern of monarchs and the distribution of these two milkweed species, this chemical evidence lends strong support to the hypothesis thatA. syriaca is the major late summer food plant of monarchs in eastern North America. This finding may be of ecological importance, forA. syriaca contributes less cardenolide and cardenolides of lower emetic potency to monarchs than most milkweeds studied to date.
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  • 36
    ISSN: 1573-1561
    Keywords: Paranthrene tabaniformis ; poplar twig clearwing moth ; sex pheromone ; Lepidoptera ; Aegeriidae ; (E,Z)-3,13-octadecadien-1-ol
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (E,Z)-3,13-Octadecadien-1-ol (la) was identified as the sex pheromone from the poplar twig clearwing moth,Paranthrene tabaniformis, females by gas chromatography-mass spectrometry analysis, synthesis, and laboratory bioassays. In the field tests, a trap baited with 200 μg synthetic la caught more male moths than two live female moths.
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  • 37
    ISSN: 1573-1561
    Keywords: Prionoxystus robiniae ; Lepidoptera ; Cossidae ; Carpenterworm ; sex attractant ; (Z,E)-3,5-tetradecadienyl acetate ; inhibition ; synergism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A new efficient stereoselective synthesis for (Z,E)-3,5-tetradecadienyl acetate (Z3,E5–14∶Ac) a potent male sex attractant for the carpenterworm,Prionoxystus robiniae (Peck), was developed. The effects of the other three isomers (Z,Z; E,Z; E,E) on the field attractiveness of theZ,E isomer toward maleP. robiniae were determined. TheZ, Z isomer inhibited attraction, theE, E isomer synergized attraction, and theE,Z isomer had no effect on attraction. Seven monounsaturated 14-carbon acetates were evaluated for their effect on the attractiveness ofZ3,E5–14∶Ac. (E)-3-Tetradecenyl acetate enhanced attraction and (Z)-9- and (E)-5-tetradecenyl acetate reduced trap captures. (Z,E)-3,5-Tetradecadien-1-ol also reduced the attractiveness ofZ3,E5–14∶Ac.
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  • 38
    ISSN: 1573-1561
    Keywords: Euphydryas anicia ; Lepidoptera ; Nymphalidae ; Castilleja integra ; Besseya plantaginea ; Besseya alpina ; iridoid glycosides ; catalpol ; macfadienoside ; sequestration ; plant-insect interactions ; herbivory ; ecological chemistry
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Iridoid glycosides were found to be sequestered by natural populations ofEuphydryas anicia after ingestion from the host plantsBesseya alpina, B. plantaginea, andCastilleja integra. Both major iridoids ofB. alpina, cataipol and aucubin, were found in butterfly populations where this was the only host plant. The catalpol-aucubin ratio was higher in the butterflies than in the host plant. AnE. anicia population which uses bothB. plantaginea andC. integra as host plants was found to sequester cataipol as well as another iridoid, macfadienoside. Macfadienoside was the major iridoid ofC. integra, while catalpol esters were the major iridoids ofB. plantaginea. Although it was a major sequestered iridoid, catalpol was a minor constituent in both host plants. The macfadienoside-catalpol ratio in the butterflies from this population was highly variable, and there appeared to be both sex and individual variation in host plant and/or iridoid glucoside utilization byE. anicia. Although other iridoids were present in the host plants, none was sequestered in more than trace amounts.
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  • 39
    ISSN: 1573-1561
    Keywords: Chemical defense ; feeding behavior ; glandular trichomes ; Helianthus ; herbivory ; Homoeosoma electellum ; Lepidoptera ; Pyralidae ; host-plant resistance ; plant-insect interactions ; sesquiterpene lactones ; terpenoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The responses of a sunflower specialist,Homoeosoma electellum, the sunflower moth, to the terpenoids produced by its host plant,Helianthus, were measured. Larvae were reared on synthetic diet containing one of three concentrations of the dominant sesquiterpene lactone found in glandular trichomes ofH. maximilliani. Treatments were initiated at each of three larval ages. Pupal weight was significantly reduced, but the effect diminished as the larvae aged. Survival and development time were unaffected by various treatments. In behavioral tests, larvae showed no preference for untreated synthetic diet compared to diet containing the secondary compound at a concentration of 1% by dry weight. When the concentration was raised to 5%, all but last-instar larvae showed a significant preference for the untreated diet. A second behavioral test measured the damage done to whole florets and an array of floral parts offered to larvae of different ages. The damage pattern of floral tissues changed as the larvae grew older, demonstrating that the willingness of larvae to eat tissues contaminated with trichome contents increased with age. The value of the glandular trichome contents as a defense againstH. electellum is discussed.
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  • 40
    ISSN: 1573-1561
    Keywords: Diaphania hyalinata ; D. nitidalis ; melonworm ; pickleworm ; Lepidoptera ; Pyralidae ; sex pheromone ; insect behavior ; flight tunnel ; 10,12-hexadecadienal ; 11-hexadecenal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ten C16 chain-length compounds were identified from heptane extracts of ovipositors of female melonworm,Diaphania hyalinata (L.). The major constituents of the extracts were (E)-11-hexadecenal and (E,E)-10,12-hexadecadienal [(E,E)-10,12–16:Ald] and the alcohols and acetates of these olefins were found in trace amounts (〈2%). Extracts also contained traces of (E,Z)- and (Z,Z)-10,12-16:Ald, hexadecanal, and 1-hexadecanol. Analysis of the behavioral responses of males to synthetic mixtures of these compounds and responses to ovipositor extracts in a flight tunnel showed that a synthetic mixture of the 10 compounds elicited a behavioral repertoire from males that was indistinguishable from that elicited by ovipositor extract. Flight tunnel studies also indicated that six of the 10 compounds probably represent the essential components of the female's sex pheromone.
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  • 41
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    Journal of chemical ecology 12 (1986), S. 1979-1986 
    ISSN: 1573-1561
    Keywords: Allium porrum L. ; Liliaceae ; Acrolepiopsis assectella Zell. ; leek-moth ; Lepidoptera ; Acrolepiidae ; ecdysone ; ecdysteroids ; 20-hydroxyecdysone ; 5,20-dihydroxyecdysone ; 20-hydroxy ; 25-deoxyecdysone ; ecdysial failures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Incorporation of certain phytoecdysones (ecdysterone, polypodine B, and ponasterone A) into a semisynthetic artificial diet induces pathophysiological effects in larvae of the leek-moth (Acrolepiopsis assectella Zell., Acrolepiidae). The effects include lethality of the newly hatched, first-instar larvae; special ecdysial failures associated with the appearance of larvae with two head capsules; and developmental anomalies during metamorphosis. The effective range of dietary ecdysteroid, as evaluated by larval mortality, varies from 25 to 250 ppm. The EC50 value is 100 ppm for polypodine B and 130 ppm for ecdysterone. The dietary effects of the phytoecdysones are similar to the previously observed effects caused by the dried flowers, but not leaves, of the leek plant. However, the active compound of the leek flowers is a saponin.
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  • 42
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    Journal of chemical ecology 12 (1986), S. 335-351 
    ISSN: 1573-1561
    Keywords: Acetate esters ; alcohols ; aldehydes ; fatty acid biosynthesis ; insect pheromones ; Lepidoptera ; pheromone biosynthesis ; spruce budworm
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The sex attractants for many Lepidoptera are long-chain, monounsaturated acetate esters, alcohols, and aldehydes. In vivo metabolic studies and enzyme analysis in vitro have provided evidence that the aldehyde pheromone of the eastern spruce budworm is synthesized de novo via an acetate ester precursor. Interconversion of the functional groups (ester, alcohol, aldehyde) can explain differences in the pheromone blends used by closely related species.
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  • 43
    ISSN: 1573-1561
    Keywords: Baldcypress ; Taxodium distichum ; 2-furaldehyde ; 2-furoic acid ; Bombyx mori ; Lepidoptera ; Bombycidae ; enteric microbial inhibition ; Bacillus ; Micrococcus ; Arthrobacter ; Mucor ; Curvularia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Exposure ofBombyx mori larvae to 2-furaldehyde, a major volatile component of baldcypress heartwood,Taxodium distichum, resulted in in vivo inhibition of enteric microorganisms at concentrations (1–47 ppm) that were released naturally from heartwood. The 7 bacterial and 2 fungal enteric isolates were also inhibited in vitro at the same concentrations. It is suggested that inhibition of leaf surface micro-organisms or in vivo inhibition of silkworm enteric microflora, as a result of indirect or direct action of 2-furaldehyde, exacerbates the growth-inhibitory effects of this compound on larvae by reducing the microbial nutritional contribution. The ecological significance of insect enteric microbial inhibition by plant allelochemics is discussed.
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  • 44
    ISSN: 1573-1561
    Keywords: Pea moth ; Cydia nigricana (F.) ; Lepidoptera ; Olethreutidae ; sex attractant ; (E)-10-dodecen-1-yl acetate ; (E,E)-8 ; 10-dodecadien-1-yl acetate ; lure ; formulation ; antioxidant ; monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Lures containing different amounts of (E)-10-dodecen-1-yl acetate and (E,E)-8, 10-dodecadien-1-yl acetate were formulated in natural rubber stoppers or polyethylene vials and tested in traps in the field to establish relative attractiveness to males of the pea moth,Cydia nigricana (F.), dose-response relationships, and the effects of weathering. Initially, both formulations of (E,E)-8,10-dodecadien-1-yl acetate were much more attractive than those of (E)-10-dodecen-1-yl acetate, but their activity diminished considerably after only 5 days in the field because an inhibitor was formed, and polyethylene formulations were not attractive after one week. However, lures containing (E)-10-dodecen-1-yl acetate, with or without antioxidants, in both rubber and polyethylene were still attractive after three months' exposure in the field and are therefore suitable for practical monitoring of pea moth populations.
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  • 45
    ISSN: 1573-1561
    Keywords: Peachtree borer ; lesser peachtree borer ; Synanthedon exitiosa ; S. pictipes ; Lepidoptera ; Sessiidae ; Z,Z-3 ; 13-octadecadien-1-ol acetate sex pheromone ; traps ; air permeation ; trap catch reduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Air permeation trials were conducted to determine whether trap catches of the peachtree borer (PTB)Synanthedon exitiosa (Say) and lesser peachtree borer (LPTB)S. pictipes (Grote and Robinson) could be reduced through application of the synthetic pheromone of the PTB, (Z,Z)-3,13-octadecadien-1-ol acetate (Z,Z-ODDA), at 92% isomeric purity. The pheromone was released into the air of peach orchard test plots with hanging laminated plastic dispensers or hollow fiber dispensers in the trees and by aerial dispersal of microcapsules containing a solution of the lure. Results showed that all formulations ofZ, Z-ODDA reduced trap catches of both PTB and LPTB in treated plots relative to catches in traps in untreated plots. In one air permeation experiment, three types of laminated dispensers and one type of hollow fiber dispenser each reduced trap catch of PTB by 93–100% and of LPTB by 75–95%. In a separate test, two formulations of microencapsulatedZ,Z-ODDA did not reduce trap catch of male PTB as effectively as the laminated dispenser; results for LPTB were similar for both the dispensers and microcapsules. Catches of male PTB moths in traps baited with laminated and or fiber pheromone dispensers containing ODDA (94∶6%Z,Z∶E,Z) were not significantly different. LPTB male moths were also captured in these traps, the catches being dependent upon the initial pheromone loading and the type of dispensers.
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  • 46
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    Journal of chemical ecology 7 (1981), S. 627-633 
    ISSN: 1573-1561
    Keywords: Insect sex pheromones ; insect sex attractants ; Lepidoptera ; pheromones ; pheromone formulations ; insect population monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The half-lives (t 1/2) of alcohol sex pheromones, 1-alkanols, acetate sex pheromones, and an epoxide (disparlure) were determined on natural rubber septa. Thet 1/2 values for the homologous alcohols from decanol to heptadecanol increased regularly from 2.2 to 1117 days, but thet 1/2 of octadecanol was 609 days. Thet 1/2 values of (Z)7-, (E)7-, and (Z)9-tetradecen-1-ol acetates were 154, 168, and 199 days, respectively, whereas those of five other tested 14-carbon acetates ranged from 310 to 350 days. The dependence oft 1/2 values on chain length and double-bond position is consistent with the hypothesis that molecular size is an important variable affectingt 1/2 values. Also, in accordance with the hypothesis, when aZ-alkenyl compound has a much shortert 1/2 than the corresponding saturated compound, thet 1/2 values of theZ compound and itsE isomer may be quite different. Thus, (E)-9-tetradecen-l-ol acetate had at 1/2 of 331 days. Thet 1/2 of disparlure was 180 days. The effect of thecis-7,8 epoxide group is apparently similar to that of the olefin group in lowering thet 1/2 below the value that would be expected solely on the basis of chain length.
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  • 47
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    Journal of chemical ecology 7 (1981), S. 733-740 
    ISSN: 1573-1561
    Keywords: Beet armyworm ; Spodoptera exigua ; Lepidoptera ; Noctuidae ; pheromone trap ; trap efficiency ; Pherocon 1C trap ; population monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relative efficiency of sticky traps and cylindrical electrical grid traps for capturing male beet armyworm moths,Spodoptera exigua (Hubner), was studied using night vision equipment. The sticky and grid traps captured 34 and 40%, respectively, of the insects which oriented to them. The grid trap's capacity was limited only by the size of the receptacle (3.8-liter bucket) at the base of the funnel. The maximum capacity of the sticky trap was ca. 110 insects, and the efficiency of this trap declined rapidly when captures exceeded 50% capacity. This decline was caused by the physical blockage of the sticky surface by the captured insects. There was no evidence to indicate that captured males had any repelling effect on other males subsequently attracted to the sticky trap.
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  • 48
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    Journal of chemical ecology 7 (1981), S. 909-917 
    ISSN: 1573-1561
    Keywords: Trichogramma pretiosum ; Hymenoptera ; Trichogrammatidae ; biological control ; kairomone ; Heliothis zea ; Lepidoptera ; Noctuidae ; host density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Trichogramma pretiosum Riley females exhibit success-motivated searching after oviposition. The stimulatory effect of contact with host eggs makes host-egg density an important factor in determining the appropriate strategy for behavioral manipulation, using kairomones, that simulate host seeking, in biological control programs. Host eggs can be used, in conjunction with kairomones or by themselves, to improve the performance of these important beneficial insects.
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  • 49
    ISSN: 1432-0878
    Keywords: Carotid body ; Calcium ; Granular vesicles ; Exocytosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An attempt was made to study the subcellular localization of calcium in carotid body glomus cells of adult rats using fixation with glutaraldehyde followed by treatment with a mixture of pyroantimonate and osmium tetroxide. Precipitates were seen as electron-dense particles (EDP) in the glomus cells, mostly within membrane-bound organelles, such as dense-cored vesicles, mitochondria, small clear vesicles, multivesicular bodies, and especially in lysosomes. However, EDP were also seen in the nuclei and in the free cytoplasm of the glomus cells and even outside them. Preincubation of carotid bodies in media containing calcium and either high potassium or calcium-ionophore A 23187 resulted in a marked increase in the general precipitation pattern, there being an increased amount of EDP both in the glomus cell nuclei and in the cytoplasm. Dense-cored vesicles more often showed precipitates than those in the controls. Some dense-cored vesicles contained multiple precipitates, typically located in the electron-lucent area between core and vesicle membrane. Extensive diffusion of ions probably occurred during fixation before precipitation, making the localization of calcium and other precipitating cations unreliable. However, it is possible that precipitates, which were regularly seen in the dense-cored vesicles, may reflect the content of bound calcium. The possible significance of calcium in glomus cell function is discussed, and the need for more adequate methods is emphasized.
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  • 50
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    Cell & tissue research 246 (1986), S. 183-187 
    ISSN: 1432-0878
    Keywords: Neonate ; Paneth cells ; Corticosteroid ; Zinc ; Calcium ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Paneth cells containing zinc-rich granules were found in the small intestine of 6-day-old rats. These cells were more numerous in older animals and were consistently most common in the distal ileum. The zinc content of granules from 10-day-old rats was similar to that found in adults (ca 300 mg atoms/kg dry weight) but no calcium could be detected. An injection of cortisone acetate at 5 days resulted in a premature increase in the numbers of Paneth cells in 10-day-old rats. The cell granules contained normal, adult levels of zinc, a calcium concentration of ca 400 mg atoms/kg dry weight and also an increased concentration of phosphorus.
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  • 51
    ISSN: 1432-0878
    Keywords: Photoreceptors ; Calcium ; Fixation artifact ; Light-adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The conditions that lead to the formation of myelin figures in rhabdomere microvilli were studied in the larval ocelli of the mosquito Aedes aegypti. These artifacts can result from the addition of divalent ions, such as Ca2+, to primary-aldehyde fixatives, but they form subsequently during postfixation with OsO4. In light-adapted ocelli, myelin figures are concentrated at the proximal ends of the microvilli along the cytoplasmic margin of the rhabdomere. The severity of the artifact is proportional to the ion concentration: scattered myelin whorls are induced by Ca2+ concentrations as low as 5 mM; they become abundant at 15 mM to 25 mM, and displace much of the rhabdomere margin at 50 mM. In contrast, even at high concentrations of Ca2+ few membrane whorls form in dark-adapted rhabdomeres, and these are mostly located at the distal ends of the microvilli. The differential response of the rhabdomere microvilli in light and darkness does not result from a direct action of light during fixation; it reflects an underlying difference between light- and dark-adapted photoreceptor membranes. We suggest that this differential sensitivity to divalent ions is associated with the shedding of membranes from the rhabdomere, a process that is enhanced by light and reduced in darkness.
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  • 52
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    Cell & tissue research 245 (1986), S. 599-604 
    ISSN: 1432-0878
    Keywords: Osmoregulation ; Mucus ; Intestine ; Biocrystallization ; Calcium ; Anguilla anguilla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Freeze-dried intestinal mucus of sea-water-adapted eels was analysed by scanning electron microscopy (SEM) and X-ray microanalysis. Calcite crystals were observed in the mucus fibres; their concentration increased along the hindgut. Random SEM observations made in situ indicated that mucus fibres were involved in the genesis of these crystals. Calcium-rich mucus globules were found fused inside crystal matrices. Single typical rhombohedric crystals of various complexity appeared within the mucus framework. The steps of crystal biogenesis were reconstituted in in-vitro conditions.
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  • 53
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    Protoplasma 130 (1986), S. 199-205 
    ISSN: 1615-6102
    Keywords: Calcium ; Crystals ; Energy dispersive X-ray analysis ; Lemna minor ; Oxalate ; Strontium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Lemna minor, which produces many calcium oxalate raphide crystals, was grown on media containing in addition to Ca, 200 μM of one of the following divalent cations: Ba, Cd, Co, Mn or Sr. Energy dispersive X-ray analysis showed that only Sr was incorporated into the raphides at levels detectable by the analysis technique. Incorporation of Sr into other insoluble compounds, such as cell wall material, could not be detected. Plant species which form different crystal types in their leaves (Beta vulgaris, crystal sand;Arthrostema ciliatum, druse;Glycine canescens, prismatic) also incorporated Sr into their crystals when grown hydroponically on nutrient medium containing 200 μM Sr. Axenic cultures ofL. minor were used to examine further the process of Sr incorporation into plant crystals. When grown on nutrient solution with 5 μM Ca, increasing the Sr concentration resulted in increases of the amount of Sr incorporated into the raphide crystals. The ratio of Sr to Ca became greater as the Sr concentration was increased. This ratio change was due to both an increase in the amount of Sr incorporated and a decrease in the Ca incorporated. Analysis of the number of crystal idioblasts formed as a function of Sr concentration shows fewer idioblasts are produced as Sr became high. Competition with Ca and interference of Ca utilization by Sr is indicated.
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  • 54
    ISSN: 1615-6102
    Keywords: Calcium ; Cell elongation ; Indole-3-acetic acid ; Pisum sativum ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The treatment of dark grown pea stem segments with chelators of divalent cations (EGTA, EDTA, CTC), various Ca2+ antagonists (LaCl3, A-23187, verapamil) and inhibitors of secretory processes (monensin, CB) reduced elongation in the presence of indole-3-acetic acid (IAA). Generally the inhibition increased with increasing concentrations of the substances. The timing of the responses can be correlated with maximum auxin-stimulated secretion of cell wall material. Examination of cell ultrastructure showed that changes in dictyosome activity could explain a reduced deposition of cell wall material and so cause inhibition of elongation. The inhibitors affected the morphology and vesiculation of the dictyosomes, and the appearance of the plasma membrane, ER and mitochondria in different ways. The most pronounced effects on ultrastructure resulted from monensin and LaCl3 treatments with the dictyosomes being most affected; large vesicles appeared in the cytoplasm. Less pronounced effects on cell structure were seen in EGTA, A-23187 and verapamil treated tissue. The effects on the dictyosomes are considered to be due to disturbances of Ca2+ and other ionic levels within the cells.
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  • 55
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    Protoplasma 109 (1981), S. 169-185 
    ISSN: 1615-6102
    Keywords: Actomyosin ; Anchorage sites ; Calcium ; Chloroplast movement ; Mougeotia sp. ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The single, ribbon-shaped chloroplast in the filamentous green algaMougeotia performs orientational movements with respect to light. The chain of reaction involves phytochrome as the photoreceptor pigment to perceive the light signal differentiated by wavelength and direction, calcium probably to convert the light signal into a chemical message and actomyosin to respond to this message and to move the chloroplast accordingly.Precise reorientation of the chloroplast is proposed to be brought about by a dual function of phytochrome: regulation of the cellular level of calciumand regulation of membrane anchorage sites to actin.
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  • 56
    ISSN: 1615-6102
    Keywords: Aureomycin ; Calcium ; Fluorescence ; Infection thread ; Root hairs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Localized regions of calcium were shown to change in clover root hair cells after infection withRhizobium trifolii as shown with the Ca++ binding antibiotic, chlorotetracycline. Some root hairs from the immature zone showed increased calcium fluorescence distributed through most of the cell. These root hair had a morphology with a notched side wall, typical of cells infected by bacteria. The maturing root hairs that show a growing infection thread or start of an infection thread, have a similar increased calcium fluorescence, especially around the area of origin of infection thread and tip of the root hair cell.
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  • 57
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    Protoplasma 132 (1986), S. 137-141 
    ISSN: 1615-6102
    Keywords: Calcium ; Chlortetracycline ; Dantrolene-Na ; Nifedipine ; Paramecium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Dantrolene-Na is a muscular relaxant which binds to sarcoplasmic reticulum (SR) with high affinity and decreases the availability of Ca2+ channels. The binding of fluorescent compounds, dantrolene-Na, nifedipine and chlortetracycline to the ciliary membrane ofParamecium aurelia has been studied. Dantrolene at the concentrations of 1.9 · 10−5, 3.8 · 10−5 and 7.9 · 10−5 M manifested a punctuated binding pattern to the cell membrane. Isolated cilia also bound dantrolene at their basal portion, whereas deciliated cell bodies lost their dotted binding pattern. Chlortetracycline showed a similar but weaker fluorescent staining. Nifedipine treated cells revealed no sign of fluorescent binding to the membrane and was only taken up in food vacuoles. Based on these observations we propose that dantrolene binding regular arrays ofParamecium cell membrane could be identical to “granular plaques” observed by electron microscope. The possible functioning of these structures as “Ca2+ reservoirs” is also discussed.
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  • 58
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    Protoplasma 132 (1986), S. 129-136 
    ISSN: 1615-6102
    Keywords: Actin ; Calcium ; Chara ; Cytoplasmic streaming ; Myosin ; Native tropomyosin ; Tropomyosin ; troponin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Native tropomyosin from rabbit skeletal muscle introduced by intracellular perfusion intoChara cells inhibited the cytoplasmic streaming irrespective of the Ca2+ concentration. To find the action site of native tropomyosin inChara, the cytoplasmic streaming was reconstituted by introducing isolated endoplasm into actin donorChara cells from which native endoplasm had been removed. The reconstituted streaming was inhibited by pretreatment of the actin donor cells with native tropomyosin but not by that of the endoplasm, suggesting that the native tropomyosin inhibited the cytoplasmic streaming by binding toChara actin bundles. Staining of the actin bundles with FITC-labeled native tropomyosin also showed that the native tropomyosin could bind to the actin bundles. Streaming reconstituted fromChara actin bundles and skeletal muscle myosin was insensitive to Ca2+, but became sensitive on application of the native tropomyosin.
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  • 59
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    Plant and soil 63 (1981), S. 491-495 
    ISSN: 1573-5036
    Keywords: Calcium ; Copper ; Flowers ; Iron ; Leaves ; Magnesium ; Nitrogen ; Nutrient uptake ; Phosphorus ; Potassium ; Koots ; Shoots ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cucumber (Cucumis sativus) plants were grown in Hoagland solution to which 20 to 2000 ppm of a soil fulvic acid (FA) were added. The addition of 100 to 300 ppm of FA produced highly significant increases in the growth and development of above and below ground plant parts, in the uptake of nutrient elements (N, P, K, Ca, Mg, Cu, Fe and Zn), and in the formation of numbers of flowers per plant. Effects of adding 500 and more ppm of FA were less beneficial.
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  • 60
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    Plant and soil 93 (1986), S. 129-132 
    ISSN: 1573-5036
    Keywords: Calcium ; Germination ; Lolium rigidum ; Magnesium chloride ; Salinity ; Sodium chloride ; Wimmera ryegrass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The modifying effect of calcium (Ca) on the salinity tolerance of Wimmera ryegrass during germination and early seedling growth was studied. Pretreatment of seeds with Ca has no significant effect on germination under NaCl or MgCl2 salinity. The addition of Ca to the germination medium increased the germination percentage significantly, especially with MgCl2. Significant increases in seedling shoot and root growth also occurred with Ca addition to the growth medium under MgCl2 salinity.
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  • 61
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    Plant and soil 93 (1986), S. 137-140 
    ISSN: 1573-5036
    Keywords: Calcium ; Magnesium ; Phosphorus ; Pinus caribaea ; Potassium ; Savanna ; Tap root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The content of elements in needles ofPinus caribaea trees growing upon infertile savanna soils was analysed at 3 and 5 years after the tap roots of a subset of trees had been severed. No significant differences were found between the content of elements in treated and control trees, nor were significant differences found in increments of diameter of the trees over 5 years. It is concluded that this organ plays no significant nutritional role in the trees on these soils, and that tree growth can be achieved exclusively from atmospheric nutrient accessions and exchangeable nutrient reserves in the topsoil.
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  • 62
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    Plant and soil 93 (1986), S. 373-382 
    ISSN: 1573-5036
    Keywords: Calcium ; Fertilizer ; Magnesium ; Nitrogen ; Nutrient content ; Phosphorus ; Pinus radiata ; Potassium ; Superphosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nutrient contents of aPinus radiata stand, that is, aboveground tree, understorey, forest floor and soil to 200 mm depth, were estimated in a replicated fertilizer trial, treated 30 years previously. The fertilizer treatments were 0, 25, 50, 75 and 100 kg P/ha as broadcast superphosphate. Highly significant growth responses had been obtained to the applied P. Estimation of the P content of the stand showed that 129%, 89%, 72% and 67% of the applied P was found in the 25, 50, 75 and 100 kg P/ha treatments respectively. The larger losses of P at the higher application rates would explain the lack of increased response with the highest application rate, however, it also shows that significant quantities of P are still present after a period of 30 years, giving a basis for growth responses in the subsequent rotations. Elevated quantities of N, Ca, Mg and K were found in the treatments compared with the control and these could not be explained on the basis of atmospheric inputs. For the cations, uptake from deeper in the soil profile could explain most of the effect but not for the higher amounts of N which, in the case of the highest superphosphate treatment, amounted to an accumulation of 27 kg N/ha/yr. These nutrient changes together with increased soil organic matter contents are considered to have provided a long term increase to the site productivity.
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  • 63
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    Plant and soil 93 (1986), S. 223-230 
    ISSN: 1573-5036
    Keywords: Boron ; Calcium ; Cell wall ; Tomato plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Tomato plants were grown in water-culture with a different supply of Ca (10, 100 ppm) and B (0, 0.2 ppm), and the effects of B deficiency on the translocation and subcellular distribution of Ca in tomato plants were studied by using45CaCl2 as a carrier of Ca. Boron deficiency slight increased the total Ca uptake by the plant and inhibited the Ca translocation to the upper leaves. The incorporation of45Ca into the cell wall in the upper leaves was increased by B deficiency at both Ca levels. As Ca supply decreased, the distribution of45Ca in the 1N NaCl fraction of the cell wall increased only at 0.2 ppm B. As B supply decreased, the distribution of45Ca in the 0.6N HCl fraction increased at both Ca levels. These results suggest that B deficiency inhibit the translocation of Ca, and induce the abnormal changes of the Ca metabolism in the cell wall.
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  • 64
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    Plant and soil 93 (1986), S. 259-268 
    ISSN: 1573-5036
    Keywords: Calcium ; Carnations ; Cation ratios ; Foot rot ; Fusarium ; Magnesium ; Nutrient solution ; Plant-sap analysis ; Potassium ; Rockwool ; Tissue analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In two experiments with carnations grown in rockwool the effects of different cation ratios in the nutrient solution were studied. The results showed that carnations need a high calcium supply. The crop did not appear to be sensitive to different potassium-magnesium ratios in the nutrient solution. In the nutrient solution added mole ratios K∶Ca∶Mg=55∶35∶10 seemed to be optimal. Such ratios in addition led to ratios of 55∶30∶15 in the root environment. Tissue analysis showed that in younger leaves of peduncles harvested a potassium content of 900 mmol per kg dry matter was optimal. For calcium a content of 350 and for magnesium 100–150 mmol per kg was needed. Analytical data of plant-sap analyses were closely correlated with data gained by digestion of dried material. For potassium and magnesium the relationships were linear. However, for calcium a curvilinear relationship was found. In the experiments indications were obtained that a sufficient calcium supply suppressed foot rot in carnations.
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  • 65
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    Journal of chemical ecology 12 (1986), S. 451-467 
    ISSN: 1573-1561
    Keywords: Cabbage looper ; Trichoplusia ni ; Lepidoptera ; Noctuidae ; insect attractant ; electrophysiology ; olfactory receptor ; pheromone blend
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Multicomponent pheromone systems are common in many insect species. As our knowledge about the number of different chemical compounds actually involved in a particular communication system increases, so too does the need for an efficient neural mechanism for the encoding of behaviorially relevant odor compounds. Here we consider the electrical activity of olfactory receptor neurons in a subset of the individual pheromone-sensitive sensilla on the antennae of male cabbage looper moths (Trichoplupia ni). Responses to single- and multiple-component stimuli, drawn from seven behaviorally active compounds, were obtained at several different intensities. Some blends elicited electrical responses which were not readily predicted from a knowledge of the receptor neuron's response to individual components.
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  • 66
    ISSN: 1573-1561
    Keywords: Summerfruit tortrix ; Adoxophyes orana ; Lepidoptera ; Tortricidae ; sex pheromone ; (Z)-9-tetradecenyl acetate ; (Z)-11-tetradecenyl acetate ; (Z)-9-tetradecen-1-ol ; (Z)-11-tetradecen-1-ol ; individual variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Twelve products related to the sex pheromone main components (Z)-9- and (Z)-11-tetradecenyl acetate (Z9–14∶Ac andZ11–14∶Ac, respectively), were identified in female pheromone gland extracts of the laboratory-reared summerfruit tortrix moth,Adoxophyes orana F.v R. These are the geometric isomers and the alcohols of the main components, (Z)-9-dodecenyl acetate, (Z)-11-hexadecenyl acetate, and saturated acetates of 12–22 carbons. The ratio ofZ9–14∶Ac toZ11–14∶Ac in individuals varied from 3.5∶1 to 11∶1 with an average of 6.2; their total added up to 462 ng/female with an average of 182 ng for 2- to 7-day-old individuals. No qualitative or quantitative differences were observed between laboratory and field insects.Z9–14∶Ac,Z11–14∶Ac and the corresponding alcohols were also found in female effluvia. Addition of either of the two alcohols to a blend of the two acetates augmented trap catch in the field. The same was true for (Z)-9,(E)-12-tetradecadienyl acetate which was not detected in gland extracts.
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  • 67
    ISSN: 1573-1561
    Keywords: Psoralen ; isopsoralen ; furanocoumarin ; Papilio polyxenes ; Lepidoptera ; Papilionidae ; metabolism ; detoxification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The comparative fate of tritiated preparations of a linear furanocoumarin (psoralen) and an angular furanocoumarin (isopsoralen) was determined in last-instar caterpillars of the black swallowtail butterfly (Papilio polyxenes Fabr.). Oral administration of either furanocoumarin at 5 μg/g is followed by rapid metabolism, primarily through oxidative cleavage of the furan ring, and the metabolites are rapidly excreted. Isopsoralen is, however, metabolized at a somewhat slower rate than is psoralen, and levels of unmetabolized isopsoralen in body tissues of the treated caterpillars are about three-fold higher. These data are compatible with the hypothesis that a reduced detoxification rate accounts at least in part for the susceptibility ofP. polyxenes caterpillars to the deleterious effect of isopsoralens.
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  • 68
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    Journal of chemical ecology 12 (1986), S. 49-68 
    ISSN: 1573-1561
    Keywords: Melanargia galathea ; Lepidoptera ; Satyridae ; marbled white butterfly ; flavonoids ; wing pigmentation ; flavonoid localization ; scales ; reproductive tissues ; sex differences ; ultraviolet radiation protection ; Oudeman's phenomenon ; sex recognition/mate selection ; chemical defense ; insect-plant interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Melanargia galathea L. from four different populations were classified into three categories, white, cream, and yellow, according to the background color of their wings. The flavonoid content of the wing and body tissues of butterflies in each of these categories was spectrophotometrically determined. A direct relationship was found between the wing background color and the flavonoid content of the wings; the greater the flavonoid content, the yellower the wings. Butterflies from the yellow category contained an average of 19.6 μg flavone/mg wing tissue; those from the cream, an average of 14.1 μg/mg tissue; and those from the white an average of 8.3 μg/mg wing tissue. Flavonoids represent on average about 1.9% by weight of the yellow wings; 1.4% of the cream wings; and about 0.8% by weight of the white wings. The most frequently encountered wing background color in each of the populations examined was cream; far fewer individuals had white or yellow wings. Flavonoids were not evenly distributed in the wings ofM. galathea; greater quantities occur on the underside of the hindwings and front margins of the forewings, while the ocelli centers of the underside of the hindwings, and the inner margins on the upperside of the hindwings lack flavonoids. Furthermore, flavonoids were found in the white scales ofM. galathea but not in the black. The flavonoid content of the body ofM. galathea was found to be much lower than that of the wings. When expressed as a percentage of that in the wings, the flavonoid content of the body of both sexes was relatively constant; however, the flavonoid content of the female body represents on average about 59% of that in the wings, compared to about 20% in the male. Both the gross flavonoid content and the flavone concentration per milligram of tissue were found to be greater in female than male butterflies. Since the concentration of flavone per cm2 of wing tissue was the same in both sexes, the greater flavonoid content of female than maleM. galathea has been attributed to the deposition of relatively large quantities in the reproductive tissues of the female. The possible role(s) of flavonoid pigments in wing coloration, in protecting vulnerable tissues from damaging ultraviolet radiation, in mate selection and/or sex recognition, in insect defense against vertebrate predators, and as antimicrobial agents inM. galathea has been discussed.
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  • 69
    ISSN: 1573-1561
    Keywords: Volatile sex pheromone blend ; behavior ; chemistry ; Heliothis virescens ; Lepidoptera ; Noctuidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Gas chromatographic and mass spectral analyses were conducted on pheromone gland extracts, volatiles collected from excised pheromone glands, and volatiles collected from calling females. In addition to tetradecanal, (Z)-9-tetradecenal, hexadecanal, (Z)-7-hexadecenal, (Z)-9-hexadecenal, and (Z)-11-hexadecenal, four other compounds, tetradecanol, (Z)-9-tetradecenol, hexadecanol, and (Z)-11-hexadecenol, were also identified from gland extracts. Only the six aldehyde components were found in gland and female volatile collections. The mean percentage of components identified from volatiles collected from calling females was 13.0% tetradecanal, 18.1% (Z)-9-tetradecenal, 7.3% hexadecanal, 0.6% (Z)-7-hexadecenal, 1.0% (Z)-9-hexadecenal, and 60.0% (Z)-11-hexadecenal. Bioassays using rubber septa formulated to release the female volatile blend indicated that all six aldehyde components play major roles in close-range male reproductive behavior. Deletion of (Z)-9-hexadecenal from the six-component blend reduced the number of copulation attempts while (Z)-7-hexadecenal exerted subtle effects on all close range behaviors. Tetradecanal affected the number of times males reorient from close range. Deletion of hexadecanal from the six-component blend resulted in a significant reduction in the number of times males landed. Comparison of the six-component synthetic blend (released at somewhat less than 1 female equivalent per hour) with calling females indicated that the six-component blend was indistinguishable from the females in inducing all of the behaviors monitored.
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  • 70
    ISSN: 1573-1561
    Keywords: Sex pheromone ; active space ; Trichopulsia ni ; Lepidoptera ; Noctuidae ; Argyrotaenia velutinana ; Grapholita molesta ; Tortricidae ; multicomponent pheromones ; sustained-flight tunnel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In the present study male redbanded leafroller (Argyrotaenia velutinana), cabbage looper (Trichoplusia ni), and Oriental fruit moths, (Grapholita molesta), were tested in a flight tunnel to (1) the major pheromone component, (2) theZ/E pheromone component mixtures for Oriental fruit moth and redbanded leafroller, (3) and the female-released blends, over a series of dosages. Experiments were designed to test the hypothesis that male response downwind of a female is initiated by the major component and that minor components function only to elicit behaviors close to the female during close-range approach and courtship. The results did not support this hypothesis, but rather showed that males initiated upwind flight in significantly higher percentages to the complete blends of components, at all dosages, compared to single components or partial blends. Addition of minor components also signficantly enhanced male perception of the major component at lower dosages, resulting in completed flights to dosages of the major component that alone did not elicit any upwind flight. Our results support the concept that minor components function to enhance male sensitivity to the pheromone, and the specificity of the signal. Our results also support the hypothesis that the active space of the pheromone is a function of the upper and lower concentration thresholds for the blend of components, and not simply for the major component.
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  • 71
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    Journal of chemical ecology 12 (1986), S. 483-496 
    ISSN: 1573-1561
    Keywords: Choristoneura fumiferana ; Lepidoptera ; Tortricidae ; pheromone components ; pheromone metabolites ; aldehyde dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In vivo incubation ofChoristoneura fumiferana with (E)-11-tetradecenal, the major pheromone component, resulted in adsorption followed by transformation to (E)-11-tetradecenoic acid, the only observed metabolite. Antennae, legs, and wings from both sexes were investigated for enzyme activity. Specificity studies with the aldehyde dehydrogenase system showed that the enzyme prefers aldehydes of 14-carbon chain length or shorter.
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    Journal of chemical ecology 12 (1986), S. 533-560 
    ISSN: 1573-1561
    Keywords: AK-reductases ; arginase ; cytochrome P-450 ; enzyme induction ; esterases ; epoxide hydrolases ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; mixed-function oxidases ; rhodanese ; transferases ; urease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A variety of oxidases, reductases, esterases, epoxide hydrolases, and group transferases in herbivorous insects and mites detoxify and facilitate the excretion of toxic phytochemicals (allelochemicals). Current theory indicates that the cytochrome P-450-dependent mixed-function oxidases (MFOs) are by far the most important enzymes because they have many attributes that are essential for an effective detoxification system. Data presented here on the midgut microsomal MFO activity of larvae of the gypsy moth,Lymantria dispar, are discussed in the light of previous work and support the theory. In the gypsy moth, the MFO levels exhibit a parallel trend with changes in specific feeding rates, and changes in the specific activity of the enzyme appear to be regulated ontogenetically and by inductive effect of chemicals in the diet. The specific activity of the MFOs rises more sharply on leaves of a highly preferred type-1 plant, the pin oak, than on an artificial wheat germ diet; the increase from mid-second instar to mid-fifth is 4.5- and 1.8-fold, respectively. The relationship of food consumption rate to increase in body mass (W) was slightly in excess of a 1∶1 ratio for both pin oak and the artificial diet, indicating that the feeding rate surpasses the increase in W (a rare phenomenon in insects). Moreover, the surface-to-volume ratios are fairly constant for combined data of gut lumen and epithelium in second to fifth instars, because the volume occupied by the epithelial cells is much larger than in older ones. Thus, it is concluded that greater specific activity of the MFO is necessary with larval advancement to higher instars in order that they may process dietary allelochemicals with an efficiency comparable to younger larvae. Additional data suggest that MFO level increases reflect further adaptation to: (1) normal, seasonal changes in plants' allelochemical composition and concentration; (2) increase in allelochemical concentration in response to leaf damage; and (3) the risk faced by dispersing larvae of encountering a greater amount and variety of allelochemicals on suboptimal/ less suitable plants. Evidence also has emerged recently for MFO-catalyzed metabolism/deactivation of numerous plant allelochemicals, including compounds that induce the enzyme. MFOs are further adapted for participation in the biogenesis of substances physiologically important to insects. Moreover, the catalytic center of the MFO system, cytochrome P-450, occurs in multiple forms; the significance of this important feature is discussed.
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  • 73
    ISSN: 1573-1561
    Keywords: Tobacco budworms ; Heliothis virescens (F) ; Lepidoptera ; Noctuidae ; tobacco ; Nicotiana tabacum L. ; diterpenes ; duvanes ; host plant resistance ; oviposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Five cuticular chemical components isolated from the green leaves of tobacco introductions (TIs) and a commercial tobacco cultivar were tested for their effects on tobacco budworm,Heliothis virescens (F), oviposition in cage bioassays, and field experiments. These chemicals were sprayed onto budworm-resistant TI 1112 tobacco which produces low levels of most cuticular components. Individual duvane diterpenes (α- and β-4,8,13-duvatrien-1-ols and α- and β-4,8,13-duvatriene-1,3-diols) increased tobacco budworm egg laying on sprayed TI 1112 plants.cis-Abienol, docosanol, and docosanyl myristate were inactive.
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  • 74
    ISSN: 1573-1561
    Keywords: Sex pheromone ; leopard moth ; Zeuzera pyrina ; Lepidoptera ; Cossidae ; (E, Z)-2 ; 13-octadecadien-1-ol acetate ; (Z)-13-octadecen-1-ol acetate
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three compounds have been identified in the abdominal tip extracts from the female leopard moth,Zeuzera pyrina L. Gas-liquid chromatography and mass spectroscopy data showed that (E, Z)-2, 13-octadecadien-1-ol acetate was the main component and that (Z)-13-octadecen-1-ol acetate and octadecan-1-ol acetate were secondary components. The electroanten-nographic responses of maleZ. pyrina to nanogram amounts of all four 2, 13-octadecadien-1-ol acetate isomers indicated that theE, Z isomer had the maximum activity. A strong EAG response was also recorded for (Z)-7-do-decen-1-ol acetate, which was not detected in the female extracts.
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  • 75
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    Journal of chemical ecology 12 (1986), S. 1247-1262 
    ISSN: 1573-1561
    Keywords: Hymenoptera ; Braconidae ; Microplitis croceipes ; parasitoid behavior ; habitat location ; Heliothis zea ; Lepidoptera ; Noctuidae ; flight tunnel ; female flights ; preflight handling
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Oriented responses ofMicroplitis croceipes (Cresson) to airborne odors of actively feedingHeliothis zea (Boddie) larvae were observed in a flight tunnel. The behavior ofM. croceipes prior to and during sustained, oriented flights was videotaped and analyzed in detail. Preflight exposure of the parasitoid to feces and other components of the plant-host complex were found to be vital in effective flight behavior, while maturation of the parasitoid had little effect. The increased frequency of oriented flight that resulted from preflight exposure of a plant-host complex persisted for at least 24 hr.
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  • 76
    ISSN: 1573-1561
    Keywords: Plant allelochemical ; parasitoids ; three trophic level interactions ; nicotine ; Manduca sexta ; Lepidoptera ; Sphingidae ; Spodoptera frugiperda ; Noctuidae ; Cotesia congregata ; Hymenoptera ; Braconidae ; Hyposoter annulipes ; Ichneumonidae
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    Notes: Abstract Parasitoids developing within tobacco hornworms or fall army-worms exhibit significant differences in development and survival depending on whether their hosts fed on nicotine-free or nicotine-containing diets. The effects of nicotine were more severe on the relatively less adapted parasitoid,H. annulipes than the specialist parasitoid,Cotesia congregata. Labeled alkaloid originally placed in herbivore diet was incorporated in several parasitoid tissues. These results suggest that interactions between plant allelochemicals and parasitoids should be considered in the development of theory on insect herbivory and plant defense.
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  • 77
    ISSN: 1573-1561
    Keywords: Grapholita funebrana ; plum fruit moth ; Grapholita molesta ; Lepidoptera ; Tortricidae ; Z-8-dodecenyl acetate ; Z-8-tetradecenyl acetate ; Z-10-tetradecenyl acetate ; sex pheromone ; delta-10-unsaturation
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    Notes: Abstract Z-8-Dodecenyl acetate (Z8–12∶Ac),E-8-dodecenyl acetate (E8–12∶Ac),Z-8-tetradecenyl acetate (Z8–14∶Ac),Z-10-tetradecenyl acetate (Z10–14∶Ac), andZ-8-dodecen-1-ol (Z8–12∶OH) were identified in the proportions 100∶1∶30∶5∶2 in female sex gland extracts ofGrapholita funebrana, accompanied by saturated acetates from 12 to 20 carbons with tetradecyl acetate predominating.Z10–14∶Ac has not previously been described as a lepidopteran sex pheromone component. Best attraction of males is obtained withZ8–12∶Ac in the presence of a higher proportion ofE8–12∶Ac than in the female. Inclusion of the 14-carbon acetates did not augmentG. funebrana catches but inhibitedG. molesta. On the other hand, addition ofZ8–12∶OH at the level optimal forG. molesta reduced attraction ofG. funebrana.
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  • 78
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    Journal of chemical ecology 12 (1986), S. 469-482 
    ISSN: 1573-1561
    Keywords: Insect ; pheromone reception ; sex pheromone ; molecular behavior ; cuticular proteins ; Antherea polyphemus ; Lepidoptera ; Saturniidae
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Body scales of the silk mothAntheraea polyphemus contain an esterase which can degrade the female sex pheromone of this species. This esterase, which appears to be stabilized to the scale cuticle, is present in both sexes, but is species specific. The enzyme may play a significant role in the behaviors associated with sex-pheromone attraction, helping to filter out stimulus noise by degrading adsorbed pheromone, thus preventing adsoptive body surfaces from becoming uncontrolled pheromone sources.
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  • 79
    ISSN: 1573-1561
    Keywords: Sugarcane stalk borer ; Chilo auricilius ; Lepidoptera ; Pyralidae ; sex pheromone ; sex attractant ; (Z)-7-dodecenyl acetate ; (Z)-8-tridecenyl acetate ; (Z)-9-tetradecenyl acetate ; (Z)-10-pentadecenyl acetate
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Four pheromonal components have been detected in ovipositor washings and volatiles from female sugarcane stalk borers,Chilo auricilius Dudgeon (Lepidoptera: Pyralidae), using combined gas chromatography-electroantennography. The components have been identified as (I) (Z)-7-do-decenyl acetate, (II) (Z)-8-tridecenyl acetate, (III) (Z)-9-tetradecenyl acetate, and (IV) (Z)-10-pentadecenyl acetate by comparison of their gas chromatographic behavior with that of synthetic standards. In field tests carried out in northern India during 1982–1984, a combination of II, III, and IV in their naturally occurring ratio (8∶4∶1) was shown to provide a highly attractive synthetic source for trap use. (Z)-7-Dodecenyl acetate was found to reduce catches of maleC. auricilius, both when dispensed with the other three components and when released from dispensers surrounding a trap baited with the other three components.
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  • 80
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    Journal of chemical ecology 7 (1981), S. 89-101 
    ISSN: 1573-1561
    Keywords: Baldcypress ; Taxodium distichum ; 2-furaldehyde ; Bombyx mori ; Lepidoptera ; Bombycidae ; growth inhibition ; toxicity ; Georgia silkworm industry
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract 2-Furaldehyde was growth inhibitory and toxic to larvae ofBombyx mori at concentrations as low as 1 ppm. Baldcypress,Taxodium distichum, heartwood released this volatile at concentrations in excess of these levels. The adverse effect of this chemical on silkworms may partially account for the demise of the Georgia silkworm industry (circa 1765), after the construction of a baldcypress rearing facility.
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  • 81
    ISSN: 1573-1561
    Keywords: Synthesis ; isomers ; 5,7-dodecadienal ; sex pheromone ; Malacosoma disstria ; Malacosoma californicum ; Lepidoptera ; Lasiocampidae
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    Notes: Abstract All four geometrical isomers of 5,7-dodecadien-1-ol have been stereoselectively synthesized by using Wittig condensation reactions. (5 Z,7E)-5,7-Dodecadien-1-ol and its corresponding aldehyde are components of the sex pheromone of the forest tent caterpillar. (5 E,7 Z)-5,7-Dodecadienal is a component of the pheromone of the western tent caterpillar. These compounds have been successfully tested in the field.
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  • 82
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    Journal of chemical ecology 7 (1981), S. 391-402 
    ISSN: 1573-1561
    Keywords: Choristoneura fumiferana ; Lepidoptera ; Tortricidae ; Abies balsamea ; feeding stimulants ; feeding deterrents ; feeding preference ; sugars ; glycosides ; amino acids ; organic acids
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Single-choice and three-choice tests were used to determine the relative importance of host-plant chemical extracts in eliciting feeding by spruce bud worm larvae. Water-soluble components of the host trees are the most important and, of these, the sugar and glycoside fractions are the most stimulating. The amino acid and organic base fractions have no apparent effect. The organic acid fractions deter feeding slightly.
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  • 83
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    Journal of chemical ecology 7 (1981), S. 615-625 
    ISSN: 1573-1561
    Keywords: Redbacked cutworm ; Euxoa ochrogaster ; Lepidoptera ; Noctuidae ; sex pheromone blend ; sex attractant ; parapheromone ; Z-5-decenyl acetate ; Z-5-undecenyl acetate ; Z-5-dodecenyl acetate ; Z-7-dodecenyl acetate ; Z-9-dodecenyl acetate
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Four acetates,Z-5-decenyl acetate,Z-5-,Z-7-, andZ-9-dodecenyl acetates, in microgram ratios of 1∶200∶2∶1 or 1∶200∶6∶2 were excellent, specific sex pheromone blends for capturing male redbacked cutworm moths in cone traps. Blends in ratios of 1∶200∶2∶1 and 2∶200∶2∶1 at 1000 μg/ rubber septum dispenser remained highly effective for 6 weeks under field conditions. The essential minor components,Z-5-decenyl,Z-7-, andZ-9-dodecenyl acetates, became inhibitory at concentrations of about 10% in the blends, and this may be an important general phenomenon in lepidopteran pheromones. Blends involving a parapheromone,Z-5-undecenyl acetate, withZ-5-,Z-7-, andZ-9-dodecenyl acetate, in microgram ratios of 8∶200∶2∶1 or 20∶200∶6∶2 were also excellent specific attractants for this species. TheZ-8-dodecenyl acetate had no obvious effect on the attraction of the redbacked cutworm males.
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  • 84
    ISSN: 1573-1561
    Keywords: Kairomone ; parasitoids ; biological control ; Trichogramma pretiosum ; Hymenoptera ; Trichogramma tidae ; Heliothis zea ; Lepidoptera ; Noeturidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The behavioral response ofTrichogramma pretiosum Riley females to the kairomone found inHeliothis zea (Boddie) moth scales is examined.
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  • 85
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    Journal of chemical ecology 7 (1981), S. 655-668 
    ISSN: 1573-1561
    Keywords: Saltmarsh caterpillar moth ; Estigmene acrea ; (Z,Z)-9,12-octadecadienal ; (Z,Z,Z)-9,12,15-octadecatrienal ; (Z,Z)-3,6-cis-9,10-epoxy-heneicosadiene ; fall webworm moth ; Hyphantria cunea ; insect sex pheromone ; cis-9,10-epoxyheneicosane ; trans-9,10-epoxyheneicosane ; linolealde-hyde ; linolenaldehyde ; Lepidoptera ; Arctiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three compounds have been identified as components of the sex pheromone emitted by females of the saltmarsh caterpillar moth,Estigmene acrea (Drury). These are (Z,Z)-9,12-octadecadienal (I), (Z,Z,Z)-9,12,15-octadecatrienal (II), and (Z,Z)-3,6-cis-9,10-epoxyheneicosadiene (III). In female tip extract they were found in a ratio of 1∶6∶25, respectively, and in trapped female effluvia the ratio was 1∶6∶27, respectively. Combinations of III with either I or II elicited sustained upwind flight in a wind tunnel, but none of these compounds by themselves did so. There is evidence that the antennal acceptor site for III is chiral.
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  • 86
    ISSN: 1573-1561
    Keywords: Disparlure ; gypsy moth ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; controlled release ; laminated dispensers ; hollow fibers ; microcapsules ; atmospheric permeation ; mating disruption ; air concentration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Disparlure, the gypsy moth sex pheromone, was aerially applied to three plots, using a different controlled-release formulation-gelatin microcapsules, flakes of plastic laminates, or hollow fibers-in each plot. Disparlure concentrations in air were measured intermittently over a 34-day period after application. Measurements were made at four heights above ground: 0.3,2,5, and 10m. Wind speed and air temperature were measured concurrently. Disparlure was emitted from the microcapsules and fibers at very high levels for the first day or two; thereafter, all three formulations produced gradually decreasing aerial concentrations. After 32–34 days, concentrations in all plots ranged from 1.5% to 15.5% of those on the first day, although most of the pheromone remained in the formulations. Results suggest that the effective life of all three formulations for mating disruption by atmospheric permeation would be near 30 days under the test conditions, with fibers likely to have the shortest life. Concentrations varied diurnally throughout the experiment, with highest levels generally between 1400 and 2200 hr each day. Concentrations were highest at the 10-m height with microcapsules and at the 0.3- and 10-m heights with flakes, but were similar at all heights with fibers. Biological implications of the results are presented.
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  • 87
    ISSN: 1573-1561
    Keywords: Pea moth ; Cydia nigricana (F.) ; Lepidoptera ; Olethreutidae ; sex attractant ; (E)-10-dodecen-1-yl acetate ; lure ; formulation ; monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Natural rubber stoppers treated with 1, 3, or 10 mg of (E)-10-dodecen-1-yl acetate, an attractant for male pea moth,Cydia nigricana (F.), were exposed in the field during the summer of 1978 and samples analyzed at intervals. The results for all three doses fitted well to first-order loss curves with half-lives of 63.5, 64.7, and 67.3 days, respectively. Thus, lures with an initial dose of 3 mg of the attractant retained approximately 1 mg after 3 months of field exposure; they should therefore maintain a constant level of attractiveness throughout this period because previous field studies showed that moths were equally responsive to fresh lures containing between 1 and 10 mg of the attractant. There was no loss of attractant during the preparation of lures or after 4 months at −15 ° C and only 13% was lost during 3 months of storage at room temperature.
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  • 88
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    Journal of chemical ecology 7 (1981), S. 1081-1088 
    ISSN: 1573-1561
    Keywords: Plusia chalcites ; Lepidoptera ; Noctuidae ; tomato looper ; sex pheromone identification ; glass WCOT capillary chromatography ; gas chromatography-mass spectrometry ; Z-7-dodencenyl acetate ; Z-9-tetra-decenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Z-7-Dodecenyl acetate (A) andZ-9-tetradecenyl acetate (B), in a 5∶1 ratio, have been identified as sex pheromone components of femalePlusia chalcites. In addition, small amounts of dodecyl acetate, tetradecyl acetate, hexadecyl acetate, andZ-9-dodecenyl acetate have been found in the abdominal tip extracts. This is the first known Plusiinae species which utilizes a two-component primary sex pheromone system. The most effective lure found in field experiments was a mixture of 1 mg A and 0.2 mg B absorbed on rubber septa.
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  • 89
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    Journal of chemical ecology 7 (1981), S. 1089-1113 
    ISSN: 1573-1561
    Keywords: Osmeterial secretion ; Papilio larvae ; Lepidoptera ; monoterpene ; sesquiterpene ; aliphatic acid ; ester
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The larval osmeterial secretions of sixPapilio species examined displayed a remarkable qualitative change at the fourth larval ecdysis. The secretions of 4th (penultimate) instar larvae ofP. machaon, P. memnon, P. helenus, P. bianor, andP. maackii principally comprised mono- and/or sesquiterpenoids. The compounds identified from these species included α-pinene, sabinene, β-myrcene, limonene, β-phellandrene, (Z)-β-ocimene, (E)-β-ocimene, β-elemene, β-caryophyllene, (E)-β-farnesene, β-selinene, (E,E)-α-farnesene, germacrene-A, germacrene-B, caryophyllene oxide, methyl 3-hydroxy-n-butyrate, and acetic acid. In contrast, the secretion of 4th larval instar ofP. xuthus, although containing similar terpenic compounds, was accompanied by large amounts of aliphatic acids and their esters: isobutyric acid, 2-methylbutyric acid, methyl isobutyrate and methyl 2-methylbutyrate. On the other hand, the osmeterial secretions of 5th (last) instar larvae varied little in quality among the six species, and the identified compounds consisted of isobutyric acid, 2-methylbutyric acid, methyl isobutyrate, ethyl isobutyrate, methyl 2-methylbutyrate, ethyl 2-methylbutyrate, and isovaleric acid, the last of which was specific toP. bianor andP. maackii. The chemical alteration of osmeterial exudate synchronized at the final larval ecdysis with the larval morphological change (particularly in body coloration) that appears to be of defensive significance against predators.
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  • 90
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Gelechiidae ; Pectinophora gossypiella ; pink bollworm ; (Z,Z)-7,11-hexadecadienyl acetate ; (Z,E)-7,11-hexadecadienyl acetate ; (Z,Z)-7,11-hexadecadienol ; (Z,E)-7,11-hexadecadienol ; pheromone analog ; behavior ; electroantennogram
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract (Z,Z)- and (Z,E)-7,11-hexadecadienol, reported to be pheromone precursors, interfere with the normal sequence of behavioral response of malePectinophora gossypiella to sex pheromone. The magnitude of the interference can be diminished with higher release rates of the sex pheromone. (Z,Z)-7,11-Hexadecadienol is more effective than itsZ,E isomer in eliciting the reduction in the behavioral response. Electroantennographic evidence suggests that each alcohol may be interfering more with receptor sites for the conformationally similar pheromone acetate than with receptor sites for the other pheromone isomer. Defining behavioral and physiological effects of pheromone analogs such as the alcohols of gossyplure may help to determine their potential for behavioral manipulations.
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  • 91
    ISSN: 1573-1561
    Keywords: Cocoa pod borer ; Conopomorpha cramerella ; Acrocercops cramerella ; Lepidoptera ; Gracillariidae ; sex pheromone ; sex attractant ; 4,6,10-hexadecatrienyl acetate ; 4,6,10-hexadecatrien-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The cocoa pod borer,Conopomorpha cramerella (Snellen) (Lepidoptera: Gracillariidae), is the most serious pest of cocoa in Southeast Asia. Analyses of ovipositor washings and entrained volatiles from virgin female moths by gas chromatography (GC) linked to electroantennography (EAG), and comparison of EAG responses from the male moth to synthetic compounds indicated the presence of theE,Z,Z andE,E,Z isomers of 4,6,10-hexadecatrienyl acetate and the corresponding alcohols, and of hexadecyl alcohol. Amounts of pheromone produced were less than 0.1 ng/female, and no peaks for the unsaturated components were observed on GC analysis. Extensive field testing of synthetic mixtures in Sabah, East Malaysia, showed that traps baited with a polyethylene vial impregnated with 1.2 mg of a mixture of the above five components in 40∶60∶4∶6∶10 ratio caught more maleC. cramerella moths than traps baited with a virgin female moth.
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  • 92
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    Journal of chemical ecology 12 (1986), S. 169-178 
    ISSN: 1573-1561
    Keywords: Iridoid glycoside ; Lepidoptera ; Euphydryas phaeton ; Nymphalidae ; Junonia coenia ; Ceratomia catalpae ; Sphingidae ; Lymantria dispar ; Lymantriidae ; chemical ecology ; insect-plant interaction ; unpalatability ; insect defense strategy
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    Notes: Abstract Thin-layer chromatography was used to follow the fates of iridoid glycosides ingested by four species of lepidopteran herbivores. These four species differed in their feeding strategy, ranging from generalist to monophagous specialist; and in their predator avoidance strategy, ranging from cryptic and palatable to aposematic and unpalatable. The fates of the iridoid glycosides ranged from sequestration by the unpalatable specialist,Euphydryas phaeton (Nymphalidae); to passage into the hemolymph and eventual elimination in the meconium by the specialistsJunonia coenia (Nymphalidae) andCeratomia catalpas (Sphingidae); to elimination of the intact compounds in the feces of the generalist feeder,Lymantria dispar (Lymantriidae).
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  • 93
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    Journal of chemical ecology 12 (1986), S. 239-249 
    ISSN: 1573-1561
    Keywords: Diaphania nitidalis ; D. hyalinata ; pickleworm ; melonworm ; Lepidoptera ; Pyralidae ; (E)-11-hexadecenal ; (E,Z)-10,12-hexadecadienal ; (E,E)-10 ; 12-hexadecadienal ; sex pheromone ; pheromonal specificity ; flight-tunnel bioassays
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Heptane extracts of the ovipositors from pickleworm adults (Diaphania nitidalis) were found to contain (E)-11-hexadecenal along with proportionally smaller amounts of (Z)-11-hexadecenal, (E)- and (Z)-11-hexadecen-1-ol, hexadecanol, hexadecanal, and a trace amount of (E,Z)-10,12-hexadecadienal. Assays conducted in a flight tunnel and in the field showed that a synthetic mixture of the five unsaturated compounds elicited behavioral responses from pickleworm males that were indistinguishable from those elicited by extracts of the female or by mate-calling females. When any component was deleted from the set of five unsaturated compounds, the intensity and extent of male responses to the resulting mixtures were significantly attenuated. The female sex pheromone of the pickleworm resembles the pheromone of a congeneric species,D. hyalinata, but bioassays indicated that (E,E)-10,12-hexadecadienal, produced byD. hyalinata but not by the pickleworm, plays a role in pheromonal specificity.
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  • 94
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    Journal of chemical ecology 12 (1986), S. 1999-2010 
    ISSN: 1573-1561
    Keywords: Oviposition stimulant ; Papilio protenor ; Lepidoptera ; Papilionidae ; flavanone glycoside ; naringin ; hesperidin ; synergy ; Citrus natsudaidai
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Identification of chemical compounds responsible for the oviposition behavior in a Rutaceae feeder,Papilio protenor demetrius, was undertaken with the epicarp of sour orange (Citrus natsudaidai) which exhibited potent stimulatory activity as did its leaves for egg-laying by the females. The stimulants were present in the hydrosoluble fraction, and the kairomonal activity displayed by the peel was regarded as originating from the synergistic effect of the total chemical complex. One of the active compounds was identified as a flavanone glycoside, naringin (naringenin-7β-neohesperidoside), which, although showing no appreciable effectiveness when bioassayed alone, elicited positive response at the concentration of 0.2% either when admixed with other unidentified components or provided the females had been conditioned with them in advance. Another flavanone glycoside, hesperidin (hesperetin-7β-rutinoside) that was contained in a trace amount in the peel also had a positive effect comparable to that of naringin under similar conditions, while their corresponding aglycones were less active or inactive. In contrast, a flavone glycoside, rhoifolin, coexisting in the peel, and some other flavones and flavonols tested as possible candidates for oviposition stimulants were all found entirely ineffective.
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  • 95
    ISSN: 1573-1561
    Keywords: Southwestern corn borer pheromone ; Diatraea grandiosella Dyar ; Lepidoptera ; Pyralidae ; (Z)-9-hexadecenal ; (Z)-11-hexadecenal ; (Z)-13-octadecenal ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We report the identification of the southwestern corn borer,Diatraea grandiosella Dyar (Lepidoptera: Pyralidae), female sex attractant pheromone as a mixture of (Z)-9-hexadecenal, (Z)-11-hexadecenal, and (Z)-13-octadecenal in the ratio 21.5∶70.6∶7.9. Initially, six 16- and 18-carbon aldehydes includingn-hexadecanal, (Z)-9-octadecenal, (Z)-11-octadecenal, and the three above were isolated from female gland rinses and evaluated as potential pheromone components by GLC-MS and laboratory bioassays. By laboratory flight chamber and field tests, the stated mixture of (Z)-9-hexadecenal, (Z)-11-hexadecenal, and (Z)-13-octadecenal was shown to be as effective as the female for male attraction. Electrophysiological studies confirmed the requirement for these three compounds, but not forn-hexadecanal, (Z)-9-octadecenal, and (Z)-11-octadecenal.
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  • 96
    ISSN: 1573-1561
    Keywords: Sabulodes caberata Guenée ; Sabulodes aegrotata Guenée ; omnivorous looper ; Lepidoptera ; Geometridae ; sex pheromone ; (Z,Z)-6,9-nonadecadiene ; (Z)-9-nonadecenekw] ; n-nonadecane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A 6,9-nonadecadiene, (Z)-9-nonadecene, andn-nonadecane were identified in extracts of sex pheromone glands of femaleSabulodes caberata Guenée. Potential pheromonal activity of gland components was assessed by electroantennogram measurements of gas-chromatographic fractions of abdominal tip extracts. Chemical identification was based on gas chromatography and mass spectrometry of parent compounds, hydrogenation products, ozonolysis products, dimethyl disulfide adducts, and synthesis. The monoene was synthesized via coupling of alkyl and acetylenic intermediates. (Z,Z)-6,9-Nonadecadiene (Z6,Z9-19∶H) was synthesized from methyl linoleate via chain lengthening. SyntheticZ6,Z9-19∶H had the same retention times on polar and nonpolar capillary gas-chromatographic columns as the 6,9-nonadecadiene from the sex pheromone glands. In field testsZ6,Z9-19:H alone produced trap catch of maleS. caberata and addition of (Z)-9-nonadecene orn-nonadecane had no effect on trap catch.
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  • 97
    ISSN: 1573-1561
    Keywords: Antherea polyphemus ; Lepidoptera ; Saturniidae ; wild silk moth ; radiolabeled pheromone ; pheromone binding ; pheromone hydrolysis ; antennal proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Sensory hair proteins from antennae of males of the wild silk moth,Antheraea polyphemus (Lepidoptera, Saturniidae) were incubated with radiolabeled 6E,11Z-hexadecadienyl acetate in the presence of unlabeled pheromone analogs as competitive inhibitors. The two extracellular proteins of importance, a highly active sensillar esterase and an abundant 15,000 mol wt binding protein, interact to degrade labeled pheromone less efficiently in the presence of certain unsaturated acetate analogs of the natural pheromone. Enzymatic hydrolysis of the acetate (or diazoacetate) was also examined for three pheromone analogs: [11,12-3H2]-6E,11Z-hexadecadienyl diazoacetate, [11,12-3H2]-hexadecyl acetate, and [9,10-3H2]-9Z-tetradecenyl acetate. The former two are poor substrates at concentrations over four orders of magnitude. The 9Z–14:Ac, however, is the best alternative substrate for this in vitro pheromone metabolism system. Unlabeled 9Z–14: Ac is also the best competitive inhibitor of the hydrolysis of labeled 6E, 11Z–16: Ac. Whereas the tritiated natural pheromone shows a flat response (ca. 40% conversion) to increasing concentrations from 3 × 10−9 to 3 × 10−6 M, tritiated 9Z–14: Ac is degraded more rapidly at higher concentrations.
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  • 98
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 12 (1986), S. 411-429 
    ISSN: 1573-1561
    Keywords: Pheromone metabolism ; tritium-labeling ; acetate esterase ; alcohol oxidase ; aldehyde dehydrogenase ; Heliothis virescens ; Lepidoptera ; Noctuidae ; sensory biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Unsaturated aliphatic pheromones ofH. virescens were prepared at high specific activity (3H, 58 Ci/mmol) and were employed to study tissue specificity of acetate esterase, alcohol oxidase, and aldehyde dehydrogenase in male and femaleHeliothis virescens. Thus, [9,10-3H2]Z9-14:Ac was synthesized by partial tritiation of the corresponding alkyne and was converted to the labeledZ9-14∶OH andZ9-14∶Al for metabolic studies. Soluble and membrane-associated enzyme activities were determined by radio-TLC assays. Esterase activity is highest in legs of both sexes, but also occurs in antennal and glandular tissues. Oxidase activity requires O2 and is highest in female pheromone gland tissues, but it is also high in the male hairpencils. Aldehyde dehydrogenase activity was uniformly high in all tissues, but highest in antennal tissues of both males and females.
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  • 99
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 12 (1986), S. 385-409 
    ISSN: 1573-1561
    Keywords: Lepidoptera ; pheromone ; chirality ; epoxide ; hydroxydanaidal ; biosynthesis ; polyene ; alkaloid ; Creatonotos transiens ; Creatonotos gangis ; Arctiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The two major components of the female sex pheromones of twoCreatonotos species have been identified as an achiral C21 triene, (Z,Z,Z)-3,6,9-heneicosatriene, and a chiral epoxide, (Z,Z)-2(2,5-octadienyl)-3-undecyloxirane. The ratios of these components in the two species fall into nonoverlapping ranges. Two additional achiral minor components, (Z,Z)-6,9-heneicosadiene and (Z,Z,Z)-3,6,9-tricosatriene, were also identified in the female sex gland extracts. The male pheromone of both species consists of hydroxydanaidal, a chiral dihydropyrrolizine derived from pyrrolizidine alkaloids in the larval diet.Creatonotos transiens was found to convert dietary heliotrine into (R)-(−)-hydroxydanaidal, with inversion at the single asymmetric carbon atom. The possible biological and biosynthetic significance of the chiral pheromone components are discussed, and they are compared with known examples of chiral lepidopteran pheromones.
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  • 100
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 12 (1986), S. 1239-1245 
    ISSN: 1573-1561
    Keywords: Amorbia cuneana ; Amorbia essigana ; sex pheromone ; sex pheromone component ratios ; Lepidoptera ; Tortricidae ; (E,E)-10,12-tetra-decadien-1-ol acetate ; (E,Z)-10,12-tetradecadien-1-ol acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The most effective lure for maleAmorbia cuneana (Walsingham) in Orange, Ventura, and Riverside counties of California was previously found to be a 1∶1 ratio of (E,E)-10,12- and (E,Z)-10,12-tetradecadien-1-ol acetates. In subsequent field tests in San Diego and Santa Barbara counties, this lure was ineffective. Analysis of sex pheromone glands (SPG) of femaleA. cuneana from these two counties showed theEE:EZ ratio to be about 1∶9 and synthetic lures of this composition were highly attractive in these areas. Analysis of the SPG of a number of females from both areas showed that there were three population types: two in the low ratio areas possessed 37 and 58%EZ, and the third in the high ratio areas possessed 89%EZ.
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