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  • Cell Line  (50)
  • Nucleic Acid Hybridization  (41)
  • American Association for the Advancement of Science (AAAS)  (81)
  • American Association for the Advancement of Science
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  • American Institute of Physics (AIP)
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  • 1985  (81)
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  • American Association for the Advancement of Science (AAAS)  (81)
  • American Association for the Advancement of Science
  • American Chemical Society
  • American Institute of Physics
  • American Institute of Physics (AIP)
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  • 1990-1994
  • 1985-1989  (81)
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  • 1
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    Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-10-25
    Beschreibung: Papillomaviruses (PV) contain several conserved genes that may encode nonstructural proteins; however, none of these predicted gene products have been identified. Papillomavirus E6 genes are retained and expressed as RNA in PV-associated human and animal carcinomas and cell lines. This suggests that the E6 gene product may be important in the maintenance of the malignant phenotype. The E6 open reading frame of the bovine papillomavirus (BPV) genome has been identified as one of two BPV genes that can independently transform mouse cells in vitro. A polypeptide encoded by this region of BPV was produced in a bacterial expression vector and used to raise antisera. The antisera specifically immunoprecipitated the predicted 15.5-kilodalton BPV E6 protein from cells transformed by the E6 gene. The E6 protein was identified in both the nuclear and membrane fractions of these transformed cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Androphy, E J -- Schiller, J T -- Lowy, D R -- 5-F32-CA-07237/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Oct 25;230(4724):442-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2996134" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Bovine papillomavirus 1/*genetics ; Cell Line ; Cell Transformation, Viral ; *Genes, Viral ; Mice ; Oncogenes ; Papillomaviridae/*genetics ; RNA, Messenger/genetics ; Rabbits ; Rats ; Tumor Virus Infections/genetics ; Viral Proteins/*genetics/isolation & purification
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 2
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    Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-11-15
    Beschreibung: The gene encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium vivax has been cloned. The deduced sequence of the protein consists of 373 amino acids with a central region of 19 tandem repeats of the nonapeptide Asp-Arg-Ala-Asp/Ala-Gly-Gln-Pro-Ala-Gly. A synthetic 18-amino acid peptide containing two tandem repeats binds to a monoclonal antibody directed to the CS protein of Plasmodium vivax and inhibits the interaction of this antibody with the native protein in sporozoite extracts. The portions of the CS gene that do not contain repeats are closely related to the corresponding regions of the CS genes of two simian malarias, Plasmodium cynomolgi and Plasmodium knowlesi. In contrast, the homology between the CS genes of Plasmodium vivax and Plasmodium falciparum, another malaria parasite of humans, is very limited.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Arnot, D E -- Barnwell, J W -- Tam, J P -- Nussenzweig, V -- Nussenzweig, R S -- Enea, V -- New York, N.Y. -- Science. 1985 Nov 15;230(4727):815-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2414847" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/immunology ; Antigens, Surface/*genetics/immunology ; Cloning, Molecular ; Epitopes/*genetics/immunology ; Haplorhini/parasitology ; Humans ; Malaria/parasitology ; Nucleic Acid Hybridization ; Plasmodium/immunology ; Plasmodium vivax/*genetics/immunology ; *Protozoan Proteins ; Repetitive Sequences, Nucleic Acid
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 3
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    Specific expression of hepatitis B surface antigen (HBsAg) in transgenic mice (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-12-06
    Beschreibung: Two transgenic mice were obtained that contain in their chromosomes the complete hepatitis B virus (HBV) genome except for the core gene. These mice secrete particles of HBV surface antigen (HBsAg) in the serum. In one mouse, HBV DNA sequences that had integrated at two different sites were shown to segregate independently in the first filial generation (F1) and only one of the sequences allowed expression of the surface antigen. Among these animals the males produced five to ten times more HBsAg than the females. A 2.1-kilobase messenger RNA species comigrating with the major surface gene messenger RNA is expressed specifically in the liver in the two original mice. The results suggest that the HBV sequences introduced into the mice are able to confer a tissue-specific expression to the S gene. In addition, the HBV transgenic mice represent a new model for the chronic carrier state of hepatitis B virus infection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Babinet, C -- Farza, H -- Morello, D -- Hadchouel, M -- Pourcel, C -- CA37300-02/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Dec 6;230(4730):1160-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3865370" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Carrier State ; DNA, Recombinant ; Female ; *Genetic Engineering ; Hepatitis B/genetics ; Hepatitis B Surface Antigens/*genetics ; Humans ; Male ; Mice ; Mice, Inbred C57BL/genetics ; Nucleic Acid Hybridization ; RNA, Messenger/genetics
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 4
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    Immunogenicity of synthetic peptides from circumsporozoite protein of Plasmodium falciparum (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-24
    Beschreibung: In a study of recombinant proteins that might be useful in developing a vaccine against malaria, synthetic peptides from the circumsporozoite (CS) protein of Plasmodium falciparum were found to be immunogenic for mice and rabbits. Antibody to peptides from the repeating region of the CS protein recognized native CS protein and blocked sporozoite invasion of human hepatoma cells in vitro. Antibodies to peptides from regions I and II had no biologic activity, although antibody to region I recognized processed CS protein by Western blot analysis. These data support the feasibility of developing a vaccine against the sporozoite stage of the malaria parasite by using synthetic peptides of the repeating region of the CS protein conjugated to a carrier protein.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ballou, W R -- Rothbard, J -- Wirtz, R A -- Gordon, D M -- Williams, J S -- Gore, R W -- Schneider, I -- Hollingdale, M R -- Beaudoin, R L -- Maloy, W L -- New York, N.Y. -- Science. 1985 May 24;228(4702):996-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2988126" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Animals ; Antibodies/immunology ; Antibody Formation ; Antigens, Surface/*immunology ; Carcinoma, Hepatocellular ; Cell Line ; Cross Reactions ; Fluorescent Antibody Technique ; Humans ; Immune Sera/immunology ; Liver Neoplasms ; Malaria/prevention & control ; Mice ; Peptides/chemical synthesis/*immunology ; Plasmodium/immunology ; Plasmodium falciparum/*immunology/physiology ; Precipitin Tests ; *Protozoan Proteins ; Rabbits ; Vaccines/immunology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 5
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    Active T-cell receptor genes have intron deoxyribonuclease hypersensitive sites (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-08-09
    Beschreibung: The T-cell receptor beta-chain gene has a nuclease hypersensitive site in several kinds of T cells, which does not appear in B cells expressing immunoglobulins. Conversely, the kappa immunoglobulin gene shows a known hypersensitive site at its enhancer element in B cells, as expected, but this site is absent in T cells. As is the case with immunoglobulin genes, the T-cell receptor site lies within the gene, in the intron separating joining and constant region segments. These nuclease hypersensitive DNA configurations in the introns of active T-cell receptor and immunoglobulin genes may arise from control elements that share ancestry but have diverged to the extent that each normally acts only in lymphoid cells which use the proximal gene product.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bier, E -- Hashimoto, Y -- Greene, M I -- Maxam, A M -- AI 19901/AI/NIAID NIH HHS/ -- CA 22427/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Aug 9;229(4713):528-34.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3927483" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; B-Lymphocytes/metabolism ; Base Sequence ; Binding Sites ; Cell Line ; Chromosome Mapping ; Collodion ; Deoxyribonuclease I/*metabolism ; Enhancer Elements, Genetic ; Gene Expression Regulation ; Humans ; Hybridomas ; Immunochemistry ; Immunoglobulin Fragments/*genetics ; Immunoglobulin Heavy Chains/genetics ; Immunoglobulin kappa-Chains/genetics ; Mice ; Receptors, Antigen, T-Cell/*genetics ; T-Lymphocytes/*metabolism ; Transcription, Genetic
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 6
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    Association of crossover points with topoisomerase I cleavage sites: a model for nonhomologous recombination (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-11-22
    Beschreibung: Nonhomologous DNA recombination is frequently observed in somatic cells upon the introduction of DNA into cells or in chromosomal events involving sequences already stably carried by the genome. In this report, the DNA sequences at the crossover points for excision of SV40 from chromosomes were shown to be associated with eukaryotic topoisomerase I cleavage sites in vitro. The precise location of the cleavage sites relative to the crossover points has suggested a general model for nonhomologous recombination mediated by topoisomerase I.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bullock, P -- Champoux, J J -- Botchan, M -- CA 30490/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Nov 22;230(4728):954-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2997924" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Base Sequence ; Cell Line ; Cell Transformation, Viral ; Chromatin/ultrastructure ; Chromosome Mapping ; DNA Topoisomerases, Type I/*metabolism ; Rats ; *Recombination, Genetic ; Simian virus 40/*genetics
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 7
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    Imaging elemental distribution and ion transport in cultured cells with ion microscopy (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-06-28
    Beschreibung: Both elemental distribution and ion transport in cultured cells have been imaged by ion microscopy. Morphological and chemical information was obtained with a spatial resolution of approximately 0.5 micron for sodium, potassium, calcium, and magnesium in freeze-fixed, cryofractured, and freeze-dried normal rat kidney cells and Chinese hamster ovary cells. Ion transport was successfully demonstrated by imaging Na+-K+ fluxes after the inhibition of Na+- and K+ -dependent adenosine triphosphatase with ouabain. This method allows measurements of elemental (isotopic) distribution to be related to cell morphology, thereby providing the means for studying ion distribution and ion transport under different physiological, pathological, and toxicological conditions in cell culture systems.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chandra, S -- Morrison, G H -- R01GM24314/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Jun 28;228(4707):1543-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2990033" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Calcium/analysis ; Cell Line ; Cells, Cultured ; Cricetinae ; Elements/*analysis ; Female ; Freeze Fracturing ; Kidney/*ultrastructure ; Magnesium/analysis ; Microscopy/methods ; Ouabain/pharmacology ; Ovary/*ultrastructure ; Potassium/analysis ; Rats ; Sodium/analysis ; Sodium-Potassium-Exchanging ATPase/antagonists & inhibitors ; Tissue Distribution
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 8
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    Disorganization of cultured vascular endothelial cell monolayers by fibrinogen fragment D (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-03-22
    Beschreibung: Fibrinogen fragment D, which is heterogeneous, has several important biological functions. Human fibrinogen fragments D94 (molecular weight, 94,000), D78 (78,000), and E (52,000) were purified. Fragments D78 and D94 but not purified fibrinogen or fragment E specifically caused disorganization of bovine aortic endothelial cells cultured as monolayers. Within 2 hours of exposure to pathophysiological concentrations of fragment D, the confluent endothelial cells retracted from each other and projected pseudopodia. These disturbed cells subsequently became rounded and detached from the substrate. The actin present in stress fibers in stationary monolayer cells was diffusely redistributed in cells with fragment D-induced alterations in morphology. This effect was not observed in monolayers of kidney epithelial cells. The results demonstrate a specific effect of fibrinogen fragment D on the disorganization of cultured vascular endothelial cell monolayers and suggest that fragment D plays a role in the pathogenesis of syndromes with vascular endothelial damage.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dang, C V -- Bell, W R -- Kaiser, D -- Wong, A -- New York, N.Y. -- Science. 1985 Mar 22;227(4693):1487-90.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/4038818" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Actins/analysis ; Animals ; Aorta ; Cattle ; Cell Adhesion/drug effects ; Cell Line ; Cells, Cultured ; Cytoskeleton/drug effects ; Endothelium/analysis/*cytology/drug effects/ultrastructure ; Epithelial Cells ; Fibrin Fibrinogen Degradation Products/*pharmacology ; Humans ; Kidney ; Pseudopodia/drug effects
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 9
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    Mammalian and yeast ras gene products: biological function in their heterologous systems (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-04-12
    Beschreibung: Activated versions of ras genes have been found in various types of malignant tumors. The normal versions of these genes are found in organisms as diverse as mammals and yeasts. Yeast cells that lack their functional ras genes, RASSC-1 and RASSC-2, are ordinarily nonviable. They have now been shown to remain viable if they carry a mammalian rasH gene. In addition, yeast-mammalian hybrid genes and a deletion mutant yeast RASSC-1 gene were shown to induce morphologic transformation of mouse NIH 3T3 cells when the genes had a point mutation analogous to one that increases the transforming activity of mammalian ras genes. The results establish the functional relevance of the yeast system to the genetics and biochemistry of cellular transformation induced by mammalian ras genes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉DeFeo-Jones, D -- Tatchell, K -- Robinson, L C -- Sigal, I S -- Vass, W C -- Lowy, D R -- Scolnick, E M -- CA37702/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Apr 12;228(4696):179-84.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3883495" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cell Transformation, Neoplastic/metabolism ; DNA, Recombinant/metabolism ; Drosophila/genetics ; Mice ; Neoplasm Proteins/*genetics/metabolism ; Nucleic Acid Hybridization ; *Oncogenes ; Plasmids ; Saccharomyces cerevisiae/*genetics
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 10
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    Inhibition of lymphocyte proliferation by a synthetic peptide homologous to retroviral envelope proteins (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-10-25
    Beschreibung: The retroviral transmembrane envelope protein p15E is immunosuppressive in that it inhibits immune responses of lymphocytes, monocytes, and macrophages. A region of p15E has been conserved among murine and feline retroviruses; a homologous region is also found in the transmembrane envelope proteins of the human retroviruses HTLV-I and HTLV-II and in a putative envelope protein encoded by an endogenous C-type human retroviral DNA. A peptide (CKS-17) was synthesized to correspond to this region of homology and was examined for its effects on lymphocyte proliferation. CKS-17 inhibited the proliferation of an interleukin-2-dependent murine cytotoxic T-cell line as well as alloantigen-stimulated proliferation of murine and human lymphocytes. Four other peptides, representing different regions of virus proteins, were inactive. These results suggest that the immunosuppressive portion of retroviral transmembrane envelope proteins may reside, at least in part, in a-conserved sequence represented by the CKS-17 peptide.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cianciolo, G J -- Copeland, T D -- Oroszlan, S -- Snyderman, R -- P01-CA29589-02/CA/NCI NIH HHS/ -- R23-CA34671-02/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Oct 25;230(4724):453-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2996136" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Animals ; Cell Line ; Deltaretrovirus/genetics ; Humans ; Leukemia Virus, Feline/genetics ; Leukemia Virus, Murine/genetics ; Lymphocyte Activation/*drug effects ; Lymphocyte Culture Test, Mixed ; Lymphocytes/drug effects ; Mice ; Mice, Inbred BALB C ; Peptides/*pharmacology ; Retroviridae/*genetics ; Spleen/cytology ; Viral Envelope Proteins/genetics/*pharmacology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 11
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    Gene for alpha-chain of human T-cell receptor: location on chromosome 14 region involved in T-cell neoplasms (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-03-01
    Beschreibung: A human complementary DNA clone specific for the alpha-chain of the T-cell receptor and a panel of rodent X human somatic cell hybrids were used to map the alpha-chain gene to human chromosome 14 in a region proximal to the immunoglobulin heavy chain locus. Analysis by means of in situ hybridization of human metaphase chromosomes served to further localize the alpha-chain gene to region 14q11q12, which is consistently involved in translocations and inversions detectable in human T-cell leukemias and lymphomas. Thus, the locus for the alpha-chain T-cell receptor may participate in oncogene activation in T-cell tumors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Croce, C M -- Isobe, M -- Palumbo, A -- Puck, J -- Ming, J -- Tweardy, D -- Erikson, J -- Davis, M -- Rovera, G -- CA 10 815/CA/NCI NIH HHS/ -- CA16685/CA/NCI NIH HHS/ -- CA215875/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Mar 1;227(4690):1044-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3919442" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Chromosome Mapping ; Chromosomes, Human, 13-15 ; DNA/genetics ; Genes ; Humans ; Hybrid Cells/metabolism ; Immunoglobulin Heavy Chains/*genetics ; Immunoglobulin alpha-Chains/*genetics ; Leukemia/genetics ; Lymphoma/genetics ; Mice ; Nucleic Acid Hybridization ; Receptors, Antigen, T-Cell/*genetics ; T-Lymphocytes ; Translocation, Genetic
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 12
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    Immunoglobulin heavy-chain enhancer requires one or more tissue-specific factors (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-18
    Beschreibung: Enhancer sequences are regulatory regions that greatly increase transcription of certain eukaryotic genes. An immunoglobulin heavy-chain variable gene segment is moved from a region lacking enhancer activity to a position adjacent to the known heavy-chain enhancer early in B-cell maturation. In lymphoid cells, the heavy-chain and SV40 enhancers bind a common factor essential for enhancer function. In contrast, fibroblast cells contain a functionally distinct factor that is used by the SV40 but not by the heavy-chain enhancer. The existence of different factors in these cells may explain the previously described lymphoid cell specificity of the heavy-chain enhancer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mercola, M -- Goverman, J -- Mirell, C -- Calame, K -- GM29361/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Jan 18;227(4684):266-70.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3917575" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antibody Formation ; B-Lymphocytes/immunology ; Base Sequence ; Cell Line ; *Enhancer Elements, Genetic ; Fibroblasts/immunology ; *Genes, Regulator ; Humans ; Immunoglobulin Constant Regions/genetics ; Immunoglobulin Heavy Chains/*genetics ; Immunoglobulin Variable Region/genetics ; Mice ; Transcription, Genetic
    Print ISSN: 0036-8075
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 13
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    AIDS virology: a battle on many fronts (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-11-01
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Norman, C -- New York, N.Y. -- Science. 1985 Nov 1;230(4725):518-21.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2413546" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acquired Immunodeficiency Syndrome/diagnosis/*microbiology ; Antibodies, Monoclonal ; Antibodies, Viral/analysis ; Antigens, Viral/analysis/immunology ; Cell Line ; Cross Reactions ; Culture Techniques/methods ; Cytopathogenic Effect, Viral ; Deltaretrovirus/immunology/*isolation & purification ; Homosexuality ; Humans ; Lymphatic Diseases/microbiology ; Microscopy, Electron ; Patents as Topic/legislation & jurisprudence ; RNA-Directed DNA Polymerase/analysis ; Reagent Kits, Diagnostic ; T-Lymphocytes/microbiology ; Terminology as Topic ; United States ; Viral Core Proteins/immunology ; Viral Envelope Proteins/immunology
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 14
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    Brain "identifier sequence" is not restricted to brain: similar abundance in nuclear RNA of other organs (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-09-20
    Beschreibung: A repeated 82 base pair sequence in genomic DNA of the rat was previously proposed as being a control element governing brain (neuron) specific genetic expression. This intronic sequence, termed the brain "identifier" (ID), is complementary to small RNA species localized in brain cytoplasm, and it was thought to be represented specifically in RNA produced by brain nuclei in vitro. The RNA blot analyses of total nuclear and polyadenylated heterogeneous nuclear RNA described in the present report show that this ID sequence is also present in the liver and kidney in abundances similar to those in the brain. This repeated sequence is not, therefore, restricted to transcripts produced in the brain as suggested from previous transcriptional "runoff" experiments. Measurements on rat and mouse nuclear RNA indicate that the abundance of ID sequence transcript is roughly proportional to the number of copies of this repeat in the respective genomes. This suggests a rather random genomic location and transcription of this sequence. From these results it seems improbable that the ID sequence functions as a transcriptional-level control element in genes expressed specifically in the brain.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Owens, G P -- Chaudhari, N -- Hahn, W E -- NS10813/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1985 Sep 20;229(4719):1263-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2412293" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Base Sequence ; *Brain Chemistry ; Cloning, Molecular ; *Genes ; Kidney/analysis ; Liver/analysis ; Mice ; Neural Crest/analysis ; Nucleic Acid Hybridization ; RNA/*analysis ; Rats ; Transcription, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 15
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    Differential control of U1 small nuclear RNA expression during mouse development (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-09-20
    Beschreibung: During normal mouse development the relative amounts of two types of U1 small nuclear RNA's (U1 RNA) change significantly. Fetal tissues have comparable levels of the two major types of mouse U1 RNA's, mU1a and mU1b, whereas most differentiated adult tissues contain only mU1a RNA's. Those adult tissues that also accumulate detectable amounts of embryonic (mU1b) RNA's (for example, testis, spleen, and thymus) contain a significant proportion of stem cells capable of further differentiation. Several strains of mice express minor sequence variants of U1 RNA's that are subject to the same developmental controls as the major types of adult and embryonic U1 RNA. The differential accumulation of embryonic U1 RNA's may influence the pattern of gene expression during early development and differentiation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lund, E -- Kahan, B -- Dahlberg, J E -- CA 33453/CA/NCI NIH HHS/ -- GM 30220/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Sep 20;229(4719):1271-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2412294" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Aging ; Animals ; Base Sequence ; Brain/*growth & development/metabolism ; Cell Line ; Embryonic and Fetal Development ; Liver/*growth & development/metabolism ; Male ; Mice ; Mice, Inbred ICR ; Neoplastic Stem Cells/metabolism ; Nucleic Acid Hybridization ; RNA/*biosynthesis ; RNA, Messenger/biosynthesis ; RNA, Small Nuclear ; Testis/*growth & development/metabolism
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 16
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    U1 small nuclear RNA genes are subject to dosage compensation in mouse cells (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-07-19
    Beschreibung: Multiple copies of a gene that encodes human U1 small nuclear RNA were introduced into mouse C127 cells with bovine papilloma virus as the vector. For some recombinant constructions, the human U1 gene copies were maintained extrachromosomally on the viral episome in an unrearranged fashion. The relative abundance of human and mouse U1 small nuclear RNA varied from one cell line to another, but in some lines human U1 RNA accounted for as much as one-third of the total U1. Regardless of the level of human U1 expression, the total amount of U1 RNA (both mouse and human) in each cell line was nearly the same relative to endogenous mouse 5S or U2 RNA. This result was obtained whether measurements were made of total cellular U1 or of only the U1 in small nuclear ribonucleoprotein particles that could be precipitated with antibody directed against the Sm antigen. The data suggest that the multigene families encoding mammalian U1 RNA are subject to some form of dosage compensation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mangin, M -- Ares, M Jr -- Weiner, A M -- GM09148/GM/NIGMS NIH HHS/ -- GM31073/GM/NIGMS NIH HHS/ -- GM31335/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Jul 19;229(4710):272-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2409601" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Autoradiography ; Bovine papillomavirus 1/genetics ; Cell Line ; DNA, Recombinant ; *Dosage Compensation, Genetic ; Electrophoresis, Polyacrylamide Gel ; Genetic Vectors ; Humans ; Mice ; Plasmids ; RNA/*genetics/isolation & purification ; RNA, Small Nuclear ; Xenopus
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 17
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    Measles virus matrix protein synthesized in a subacute sclerosing panencephalitis cell line (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-06-07
    Beschreibung: Measles virus generally produces acute illness. Rarely, however, persistent infection of brain cells occurs, resulting in a chronic and fatal neurological disease, subacute sclerosing panencephalitis (SSPE). Evidence indicates that expression of the measles virus matrix protein is selectively restricted in this persistent infection, but the mechanism underlying this restriction has not been identified. Defective translation of matrix messenger RNA has been described in one SSPE cell line. This report presents evidence that in a different SSPE tissue culture cell line IP-3-Ca, the matrix protein is synthesized but fails to accumulate. A general scheme is proposed to reconcile the different levels at which restriction of matrix protein has been observed.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sheppard, R D -- Raine, C S -- Bornstein, M B -- Udem, S A -- CA13330-12/CA/NCI NIH HHS/ -- NS 08952/NS/NINDS NIH HHS/ -- NS 11920/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1985 Jun 7;228(4704):1219-21.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/4001938" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Cell Line ; Gene Expression Regulation ; Humans ; Hydrolysis ; Measles virus/genetics/growth & development/*metabolism ; Molecular Weight ; Mutation ; Protein Processing, Post-Translational ; Subacute Sclerosing Panencephalitis/*microbiology ; Viral Matrix Proteins ; Viral Proteins/*biosynthesis/genetics ; Virus Replication
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 18
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    Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-12-20
    Beschreibung: Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase (220,000 times) of target DNA copies. In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end-labeled oligonucleotide probe hybridized in solution to the amplified beta-globin sequences. The beta-globin genotype can be determined in less than 1 day on samples containing significantly less than 1 microgram of genomic DNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Saiki, R K -- Scharf, S -- Faloona, F -- Mullis, K B -- Horn, G T -- Erlich, H A -- Arnheim, N -- New York, N.Y. -- Science. 1985 Dec 20;230(4732):1350-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2999980" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Alleles ; Anemia, Sickle Cell/*diagnosis/genetics ; Base Sequence ; Clinical Laboratory Techniques ; DNA Restriction Enzymes ; DNA-Directed DNA Polymerase ; Escherichia coli ; *Gene Amplification ; Globins/*genetics ; Humans ; Nucleic Acid Hybridization ; Polymorphism, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 19
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    Studies of the putative transforming protein of the type I human T-cell leukemia virus (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-06-21
    Beschreibung: The putative transforming protein of the type I human T-cell leukemia virus (HTLV-1) is a 40-kilodalton protein encoded by the X region and is termed p40XI. On the basis of both subcellular fractionation techniques and immunocytochemical analysis, it is now shown that p40XI is a nuclear protein with a relatively short half-life (120 minutes). It is synthesized de novo in considerable quantities in a human T-cell line infected with and transformed by the virus in vitro, and it is not packaged in detectable amounts in the extracellular virus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Slamon, D J -- Press, M F -- Souza, L M -- Murdock, D C -- Cline, M J -- Golde, D W -- Gasson, J C -- Chen, I S -- CA 32737/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Jun 21;228(4706):1427-30.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2990027" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Antigens, Polyomavirus Transforming ; Antigens, Viral, Tumor/immunology/*metabolism ; Cell Fractionation ; Cell Line ; Cell Nucleus/metabolism ; Cell Transformation, Viral ; Deltaretrovirus/*metabolism ; Half-Life ; Humans ; Immune Sera ; Precipitin Tests ; Viral Proteins/immunology/*metabolism
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 20
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    Dissociation of antitumor potency from anthracycline cardiotoxicity in a doxorubicin analog (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-06-28
    Beschreibung: The search for new congeners of the leading anticancer drug doxorubicin has led to an analog that is approximately 1000 times more potent, noncardiotoxic at therapeutic dose levels, and non-cross-resistant with doxorubicin. The new anthracycline, 3'-deamino-3'-(3-cyano-4-morpholinyl)doxorubicin (MRA-CN), is produced by incorporation of the 3' amino group of doxorubicin in a new cyanomorpholinyl ring. The marked increase in potency was observed against human ovarian and breast carcinomas in vitro; it was not accompanied by an increase in cardiotoxicity in fetal mouse heart cultures. Doxorubicin and MRA-CN both produced typical cardiac ultrastructural and biochemical changes, but at equimolar concentrations. In addition, MRA-CN was not cross-resistant with doxorubicin in a variant of the human sarcoma cell line MES-SA selected for resistance to doxorubicin. Thus antitumor efficacy was dissociated from both cardiotoxicity and cross-resistance by this modification of anthracycline structure.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sikic, B I -- Ehsan, M N -- Harker, W G -- Friend, N F -- Brown, B W -- Newman, R A -- Hacker, M P -- Acton, E M -- CA 24543/CA/NCI NIH HHS/ -- CA 32250/CA/NCI NIH HHS/ -- CA 33303/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Jun 28;228(4707):1544-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/4012308" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Antineoplastic Agents ; Breast Neoplasms/drug therapy ; Cell Line ; Chemical Phenomena ; Chemistry ; Dose-Response Relationship, Drug ; Doxorubicin/adverse effects/*analogs & derivatives/therapeutic use ; Female ; Heart/drug effects ; Humans ; Isoenzymes ; L-Lactate Dehydrogenase/analysis ; Mice ; Myocardium/enzymology ; Ovarian Neoplasms/drug therapy ; Pregnancy
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 21
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    Trans-acting transcriptional regulation of human T-cell leukemia virus type III long terminal repeat (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-11
    Beschreibung: Human T-cell leukemia virus type III (HTLV-III) was recently identified as the probable etiologic agent of the acquired immune deficiency syndrome (AIDS). Here it is shown that, in human T-cell lines infected with HTLV-III, gene expression directed by the long terminal repeat sequence of this virus is stimulated by more than two orders of magnitude compared to matched uninfected cells. The rate of transcription of the HTLV-III long terminal repeat is more than 1000 times that of the SV40 early promoter in one infected cell line. Thus, HTLV-III, like HTLV-I, HTLV-II, and the bovine leukemia virus, is characterized by trans-activation of transcription in infected cells. The efficiency of trans-activation in the case of HTLV-III may account, at least in part, for the virulent nature of HTLV-III infection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sodroski, J -- Rosen, C -- Wong-Staal, F -- Salahuddin, S Z -- Popovic, M -- Arya, S -- Gallo, R C -- Haseltine, W A -- CA07094/CA/NCI NIH HHS/ -- CA07580/CA/NCI NIH HHS/ -- CA36974/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Jan 11;227(4683):171-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2981427" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acetyltransferases/genetics/metabolism ; Cell Line ; Chloramphenicol O-Acetyltransferase ; DNA, Recombinant ; Deltaretrovirus/*genetics ; *Gene Expression Regulation ; Humans ; Operon ; Plasmids ; Transcription, Genetic ; Transfection
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 22
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    Transposon tagging to detect a latent virus in Myxococcus xanthus (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-11-01
    Beschreibung: Transposon mutagenesis of the bacterium Myxococcus xanthus with the transposon Tn5 revealed a special class of bacterial mutants that transduced the transposon through culture supernatant fluids. Virus-like particles copurified with transducing activity. Transposon tagging for detecting these virus-like particles may be generally useful in isolating endogenous viral agents capable of transferring genetic information between cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Starich, T -- Cordes, P -- Zissler, J -- CA 09138/CA/NCI NIH HHS/ -- GM 19557/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Nov 1;230(4725):541-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2996138" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Bacteriophages/*analysis ; Centrifugation, Isopycnic ; *DNA Transposable Elements ; Microscopy, Electron ; *Mutation ; Myxococcales/*genetics ; Nucleic Acid Hybridization ; Virion/analysis
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 23
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    Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-11-22
    Beschreibung: Modulation of the growth of human and murine cell lines in vitro by recombinant human tumor necrosis factor-alpha (rTNF-alpha) and recombinant human interferon-gamma (rIFN-gamma) was investigated. rTNF-alpha had cytostatic or cytolytic effects on only some tumor cell lines. When administered together with rIFN-gamma, rTNF-alpha showed enhanced antiproliferative effects on a subset of the cell lines tested. In contrast to its effects on sensitive tumor cells, rTNF-alpha augmented the growth of normal diploid fibroblasts. Variations in the proliferative response induced by rTNF-alpha were apparently not due to differences in either the number of binding sites per cell or their affinity for rTNF-alpha. These observations indicate that the effects of rTNF-alpha on cell growth are not limited to tumor cells, but rather that this protein may have a broad spectrum of activities in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sugarman, B J -- Aggarwal, B B -- Hass, P E -- Figari, I S -- Palladino, M A Jr -- Shepard, H M -- New York, N.Y. -- Science. 1985 Nov 22;230(4728):943-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3933111" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cell Division/*drug effects ; Cell Line ; Cell Survival/drug effects ; Cell Transformation, Neoplastic/pathology ; Drug Synergism ; Glycoproteins/*pharmacology ; Humans ; Interferon-gamma/pharmacology ; Mice ; Recombinant Proteins/*pharmacology ; Tumor Necrosis Factor-alpha
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 24
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    Reversal of oncogenesis by the expression of a major histocompatibility complex class I gene (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-04-05
    Beschreibung: The classical transplantation antigens (the major histocompatibility complex class I antigens) play a key role in host defense against cells expressing foreign antigens. Several naturally occurring tumors and virally transformed cells show an overall suppression of these surface antigens. Since the class I molecules are required in the presentation of neoantigens on tumor cells to the cytotoxic T lymphocytes, their absence from the cell surface may lead to the escape of these tumors from immunosurveillance. To test this possibility, a functional class I gene was transfected into human adenovirus 12-transformed mouse cells that do not express detectable levels of class I antigens; the transformants were tested for expression of the transfected gene and for changes in oncogenicity. The expression of a single class I gene, introduced by DNA-mediated gene transfer into highly tumorigenic adenovirus 12-transformed cells, was sufficient to abrogate the oncogenicity of these cells. This finding has important implications for the regulation of the malignant phenotype in certain tumors and for the potential modulation of oncogenicity through derepression of the endogenous class I genes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tanaka, K -- Isselbacher, K J -- Khoury, G -- Jay, G -- New York, N.Y. -- Science. 1985 Apr 5;228(4695):26-30.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3975631" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antigens, Neoplasm/immunology ; Cell Line ; Immunization ; *Major Histocompatibility Complex ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Neoplasm Transplantation ; Neoplasms, Experimental/genetics/*immunology ; Rats
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 25
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    Bovine leukemia virus-related antigens in lymphocyte cultures infected with AIDS-associated viruses (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-03-22
    Beschreibung: An earlier finding that lymphocytes from African patients with the acquired immune deficiency syndrome (AIDS) react with rabbit antiserum to purified antigens of bovine leukemia virus (BLV) prompted a study of the possible cross-reactions between a BLV-infected ovine cell line and human lymphocytes inoculated with a strain of lymphadenopathy syndrome-associated virus (LAV). A solid-phase radioimmunoassay was used to detect antigenic markers of the retroviruses. Crude extracts from short-term cultures of lymphocytes infected with LAV bound rabbit antisera to the LAV glycoprotein gp13 (molecular weight 13,000) and the BLV proteins p24 and gp51, but did not bind antibodies to the p24 of human T-cell leukemia virus type I (HTLV-I). Antiserum to LAV gp13 reacted with an ovine cell line producing BLV but also weakly with virus-free ovine cells. Lymphocyte cultures from four African patients with AIDS expressed BLV-related antigens within 6 to 10 days of culture, at the moment when particle-bound reverse transcriptase was produced. BLV-related antigens were induced in lymphocyte cultures from healthy individuals by addition of filtered supernatant or irradiated cells of the original culture. The antisera to BLV used in this study may prove useful for the detection of AIDS-associated viruses in short-term cultures of lymphocytes from AIDS patients or their contacts.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Thiry, L -- Sprecher-Goldberger, S -- Jacquemin, P -- Cogniaux, J -- Burny, A -- Bruck, C -- Portetelle, D -- Cran, S -- Clumeck, N -- New York, N.Y. -- Science. 1985 Mar 22;227(4693):1482-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2579433" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acquired Immunodeficiency Syndrome/*microbiology ; Animals ; Antigens, Viral/analysis/*immunology ; Cell Line ; Cells, Cultured ; Cross Reactions ; Deltaretrovirus/*immunology ; Epitopes/immunology ; Humans ; Leukemia Virus, Bovine/*immunology ; Lymph Nodes/microbiology ; Lymphocytes/immunology/*microbiology ; Radioimmunoassay ; Retroviridae/*immunology ; Sheep ; Viral Proteins/immunology
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 26
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    Cloning of a gene whose expression is increased in scrapie and in senile plaques in human brain (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-12-06
    Beschreibung: A complementary DNA library was constructed from messenger RNA's extracted from the brains of mice infected with the scrapie agent. The library was differentially screened with the objectives of finding clones that might be used as markers of infection and finding clones of genes whose increased expression might be correlated with the pathological changes common to scrapie and Alzheimer's disease. A gene was identified whose expression is increased in scrapie. The complementary DNA corresponding to this gene hybridized preferentially and focally to cells in the brains of scrapie-infected animals. The cloned DNA also hybridized to the neuritic plaques found with increased frequency in brains of patients with Alzheimer's disease.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wietgrefe, S -- Zupancic, M -- Haase, A -- Chesebro, B -- Race, R -- Frey, W 2nd -- Rustan, T -- Friedman, R L -- New York, N.Y. -- Science. 1985 Dec 6;230(4730):1177-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3840915" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Alzheimer Disease/*genetics/pathology ; Animals ; Brain/*metabolism/pathology ; Cloning, Molecular ; Cricetinae ; DNA/genetics ; Humans ; Mice ; Nucleic Acid Hybridization ; RNA, Messenger/genetics ; Scrapie/*genetics/pathology ; Sheep
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 27
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    Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-17
    Beschreibung: Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wong, G G -- Witek, J S -- Temple, P A -- Wilkens, K M -- Leary, A C -- Luxenberg, D P -- Jones, S S -- Brown, E L -- Kay, R M -- Orr, E C -- New York, N.Y. -- Science. 1985 May 17;228(4701):810-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3923623" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Animals ; Base Sequence ; Cell Line ; *Cloning, Molecular ; Colony-Stimulating Factors/biosynthesis/*genetics/isolation & purification ; *Dna ; DNA, Recombinant ; *Granulocytes ; Haplorhini ; Humans ; *Macrophages ; RNA, Messenger/genetics ; T-Lymphocytes ; Transfection
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 28
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    Gene for human insulin receptor: localization to site on chromosome 19 involved in pre-B-cell leukemia (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-10
    Beschreibung: Consistent chromosomal translocations in neoplastic cells may alter the expression of proto-oncogenes that are located near the breakpoints. The complementary DNA sequence of the human insulin receptor is similar to those of the EGF receptor (erbB oncogene) and products of the src family of oncogenes. With in situ hybridization and Southern blot analysis of somatic cell hybrid DNA, the human insulin receptor gene was mapped to the distal short arm of chromosome 19 (bands p13.2----p13.3), a site involved in a nonrandom translocation in pre-B-cell acute leukemia.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yang-Feng, T L -- Francke, U -- Ullrich, A -- GM 26105/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 May 10;228(4700):728-31.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3873110" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; B-Lymphocytes ; *Chromosome Mapping ; *Chromosomes, Human, 19-20 ; Cricetinae ; Cricetulus ; Humans ; Hybrid Cells/metabolism ; Leukemia, Lymphoid/*genetics ; Nucleic Acid Hybridization ; Receptor, Insulin/*genetics ; Translocation, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 29
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    Transformation of human bronchial epithelial cells transfected by Harvey ras oncogene (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-03-08
    Beschreibung: Transfection of normal human bronchial epithelial (NHBE) cells with a plasmid carrying the ras oncogene of Harvey murine sarcoma virus (v-Ha ras) changed the growth requirements, terminal differentiation, and tumorigenicity of the recipient cells. One of the cell lines isolated after transfection (TBE-1) was studied extensively and shown to contain v-Ha ras DNA. Total cellular RNA from TBE-1 cells hybridized to v-Ha ras structural gene fragment probes five to eight times more than RNA from parental NHBE cells. The TBE-1 cells expressed phosphorylated v-Ha ras polypeptide p21, showed a reduced requirement for growth-factor supplements, and became aneuploid as an early cellular response to v-Ha ras expression. As the transfectants acquire an indefinite life-span and anchorage independence they became transplantable tumor cells and showed many phenotypic changes suggesting a pleiotropic mechanism for the role of Ha ras in human carcinogenesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yoakum, G H -- Lechner, J F -- Gabrielson, E W -- Korba, B E -- Malan-Shibley, L -- Willey, J C -- Valerio, M G -- Shamsuddin, A M -- Trump, B F -- Harris, C C -- New York, N.Y. -- Science. 1985 Mar 8;227(4691):1174-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3975607" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Bronchi/*cytology/microbiology ; Carcinoma, Bronchogenic/genetics ; Cell Line ; Cell Transformation, Neoplastic/metabolism ; *Cell Transformation, Viral ; Culture Media ; DNA, Neoplasm/genetics ; Epithelial Cells ; Epithelium/microbiology ; Humans ; Lung Neoplasms/genetics ; Mice ; Mice, Nude ; Nucleic Acid Hybridization ; *Oncogenes ; Rats ; *Transfection
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 30
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    Expression of Plasmodium falciparum circumsporozoite proteins in Escherichia coli for potential use in a human malaria vaccine (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-24
    Beschreibung: The circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum may be the most promising target for the development of a malaria vaccine. In this study, proteins composed of 16, 32, or 48 tandem copies of a tetrapeptide repeating sequence found in the CS protein were efficiently expressed in the bacterium Escherichia coli. When injected into mice, these recombinant products resulted in the production of high titers of antibodies that reacted with the authentic CS protein on live sporozoites and blocked sporozoite invasion of human hepatoma cells in vitro. These CS protein derivatives are therefore candidates for a human malaria vaccine.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Young, J F -- Hockmeyer, W T -- Gross, M -- Ballou, W R -- Wirtz, R A -- Trosper, J H -- Beaudoin, R L -- Hollingdale, M R -- Miller, L H -- Diggs, C L -- New York, N.Y. -- Science. 1985 May 24;228(4702):958-62.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2988125" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Antibody Formation ; Antigens, Surface/genetics/*immunology ; Carcinoma, Hepatocellular ; Cell Line ; Cloning, Molecular ; Cross Reactions ; DNA, Recombinant ; Escherichia coli/genetics ; Humans ; Liver Neoplasms ; Malaria/*prevention & control ; Mice ; Plasmodium/immunology ; Plasmodium falciparum/genetics/*immunology/physiology ; *Protozoan Proteins ; Vaccines/*immunology
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 31
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    Superinduction of c-fos by nerve growth factor in the presence of peripherally active benzodiazepines (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-09-20
    Beschreibung: Alterations in proto-oncogene expression after stimulation of rat pheochromocytoma (PC12) cells by nerve growth factor (NGF) have been investigated. A specific stimulation of c-fos messenger RNA and protein was detected 30 minutes after treatment. This induction was enhanced more than 100-fold in the presence of peripherally active benzodiazepines. The effect was specific as very little change was observed in the levels of c-rasHa, c-rasKi, c-myc, and N-myc messenger RNA's. Under the conditions used here, NGF treatment ultimately results in neurite outgrowth, with a reduction or cessation of cell division. Thus, stimulation of the c-fos gene in this system appeared to be associated with differentiation and not with cellular proliferation. The effect of benzodiazepines was stereospecific and represents a novel action of these compounds at the level of gene expression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Curran, T -- Morgan, J I -- New York, N.Y. -- Science. 1985 Sep 20;229(4719):1265-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/4035354" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Benzodiazepines/*pharmacology ; Cell Line ; Cell Transformation, Neoplastic/drug effects ; Gene Expression Regulation/*drug effects ; Nerve Growth Factors/*pharmacology ; *Oncogenes ; Pheochromocytoma/genetics/metabolism ; RNA, Messenger/biosynthesis ; Rats
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 32
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    The role of the c-mos gene in the 8;21 translocation in human acute myeloblastic leukemia (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-08-23
    Beschreibung: The human c-mos proto-oncogene is located on chromosome 8 at band q22, close to the breakpoint in the t(8;21) (q22;q22) chromosome rearrangement. This translocation is associated with acute myeloblastic leukemia, subgroup M2. The c-myc gene, another proto-oncogene, has been mapped to 8q24. The breakpoint at 8q22 separates these genes, as determined by in situ hybridization of c-mos and c-myc probes. The c-mos gene remains on the 8q-chromosome and the c-myc gene is translocated to the 21q+ chromosome. Southern blot analysis of DNA from bone marrow cells of four patients with this translocation showed no rearrangement of c-mos.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Diaz, M O -- Le Beau, M M -- Rowley, J D -- Drabkin, H A -- Patterson, D -- CA 16910/CA/NCI NIH HHS/ -- CA 25568/CA/NCI NIH HHS/ -- HD 13432/HD/NICHD NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1985 Aug 23;229(4715):767-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3860954" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Chromosome Mapping ; *Chromosomes, Human, 21-22 and Y ; *Chromosomes, Human, 6-12 and X ; Humans ; Leukemia, Myeloid, Acute/*genetics ; Nucleic Acid Hybridization ; *Oncogenes ; *Translocation, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 33
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    Contribution of promoter to tissue-specific expression of the mouse immunoglobulin kappa gene (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-09-13
    Beschreibung: The immunoglobulin kappa (kappa) gene promoter was activated by a "neutral" enhancer derived from Harvey murine sarcoma virus (HaMuSV) in immunoglobulin-producing myeloma cells, regardless of the enhancer's orientation or position in the vector. In one fibroblast line (3T3) the immunoglobulin kappa gene promoter was completely inactive when linked to the HaMuSV enhancer, whereas in mouse L cells, promoter activity was observed only with the HaMuSV enhancer in tandem with the immunoglobulin kappa gene promoter. The differential behavior of the gene promoter, when activated by a neutral enhancer in these three murine cell lines, suggests that promoter sequences contribute to the tissue-specific expression of this gene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gopal, T V -- Shimada, T -- Baur, A W -- Nienhuis, A W -- New York, N.Y. -- Science. 1985 Sep 13;229(4718):1102-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2994213" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cell Line ; Cell Transformation, Viral ; Fibroblasts/analysis ; *Gene Expression Regulation ; Immunoglobulin Light Chains/*genetics ; Immunoglobulin kappa-Chains/*genetics ; Mice ; Multiple Myeloma/metabolism ; *Operon ; Sarcoma Viruses, Murine
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 34
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    Spatially regulated expression of homeotic genes in Drosophila (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-09-20
    Beschreibung: The sites of transcript accumulation for six different homeotic loci of the Antennapedia and bithorax gene complexes (ANT-C and BX-C) were identified within embryo tissue sections by in situ hybridization. These six loci belong to the Antennapedia class of the homeo box gene family. Transcripts encoded by each locus are detected primarily in discrete, nonoverlapping regions of the embryonic central nervous system (CNS). The regions of the CNS that contain transcripts encoded by each of these loci correspond to the embryonic segments that are disrupted in mutants for these genes. The maintenance of spatially restricted expression of each ANT-C and BX-C locus could involve hierarchical, cross-regulatory interactions that are mediated by the homeo box protein domains encoded by these genes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Harding, K -- Wedeen, C -- McGinnis, W -- Levine, M -- New York, N.Y. -- Science. 1985 Sep 20;229(4719):1236-42.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3898362" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Central Nervous System/growth & development ; Chromosome Mapping ; Cloning, Molecular ; Drosophila/*genetics/growth & development/physiology ; *Gene Expression Regulation ; *Genes ; Nucleic Acid Hybridization ; Transcription, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 35
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    cis- and trans-acting transcriptional regulation of visna virus (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-08-02
    Beschreibung: Visna virus is a pathogenic lentivirus of sheep that is related to human T-cell lymphotropic virus type III (HTLV-III), the probable etiologic agent of the acquired immune deficiency syndrome (AIDS). The transcriptional activity of visna virus promoter and enhancer sequences was studied by means of an assay based on the transient expression of the bacterial gene chloramphenicol acetyltransferase (CAT). The results suggest that the high level of expression of visna virus is due in part to cis-acting enhancer sequences that give the viral promoter a high level of transcriptional activity. In addition, the rate of transcription from the visna virus promoter situated in a plasmid expressing the CAT gene was much greater in infected than uninfected cells. This phenomenon of trans-acting transcriptional activation may involve either virally or cellularly encoded factors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hess, J L -- Clements, J E -- Narayan, O -- NS-15721/NS/NINDS NIH HHS/ -- NS-16145/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1985 Aug 2;229(4712):482-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2990051" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acetyltransferases/genetics ; Animals ; Base Sequence ; Cell Line ; Chloramphenicol O-Acetyltransferase ; Choroid Plexus ; Chromosome Mapping ; Enhancer Elements, Genetic ; *Genes, Regulator ; Goats ; Humans ; L Cells (Cell Line) ; Macrophages ; Mice ; Plasmids ; Promoter Regions, Genetic ; Sheep ; Synovial Membrane ; T-Lymphocytes ; *Transcription, Genetic ; Transfection ; Visna-maedi virus/*genetics
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 36
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    The human gene encoding GM-CSF is at 5q21-q32, the chromosome region deleted in the 5q- anomaly (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-12-13
    Beschreibung: Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. A full-length complementary DNA clone encoding human GM-CSF was used as a probe to screen a human genomic library and isolate the gene encoding human GM-CSF. The human GM-CSF gene is approximately 2.5 kilobase pairs in length with at least three intervening sequences. The GM-CSF gene was localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. An established, human promyelocytic leukemia cell line, HL60, contains a rearranged, partially deleted GM-CSF allele and a candidate 5q- marker chromosome, indicating that the truncated GM-CSF allele may reside at the rejoining point for the interstitial deletion on the HL60 marker chromosome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Huebner, K -- Isobe, M -- Croce, C M -- Golde, D W -- Kaufman, S E -- Gasson, J C -- CA-10805/CA/NCI NIH HHS/ -- CA-16685/CA/NCI NIH HHS/ -- CA-21124/CA/NCI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1985 Dec 13;230(4731):1282-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2999978" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Anemia/genetics ; Base Sequence ; Cell Line ; Chromosome Aberrations/*genetics ; Chromosome Deletion ; Chromosome Disorders ; Chromosome Mapping ; *Chromosomes, Human, 4-5 ; Colony-Stimulating Factors/*genetics ; DNA Restriction Enzymes ; Genes ; Granulocytes ; Humans ; Leukemia, Myeloid, Acute/genetics ; Macrophages ; Syndrome
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 37
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    Promoter region of the human Harvey ras proto-oncogene: similarity to the EGF receptor proto-oncogene promoter (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-12-20
    Beschreibung: Regulation of transcription of members of the ras gene family undoubtably plays an important role in controlling cellular growth. Examination of this level of regulation requires identification of the promoter regions of the ras proto-oncogenes. Four major transcriptional start sites were detected in the human Harvey ras 1 proto-oncogene. The promoter region contains neither a TATA box nor a CAAT box in their characteristic upstream positions, has an extremely high G+C content (80 percent), and contains multiple GC boxes including seven CCGCCC repeats and three repeats of the inverted complement, GGGCGG. This region has strong promoter activity when placed upstream from the chloramphenicol acetyl transferase gene and transfected into monkey CV1 cells. In these ways the Harvey ras 1 proto-oncogene promoter resembles the promoter of the gene encoding the epidermal growth factor (EGF) receptor. The similarity between the two proto-oncogene promoters may be relevant to the mechanism by which the expression of such "growth control" genes is regulated.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ishii, S -- Merlino, G T -- Pastan, I -- New York, N.Y. -- Science. 1985 Dec 20;230(4732):1378-81.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2999983" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): DNA Restriction Enzymes ; Epidermal Growth Factor/metabolism ; *Genes ; Humans ; Nucleic Acid Hybridization ; Plasmids ; *Promoter Regions, Genetic ; *Proto-Oncogenes ; RNA, Messenger/genetics ; Receptor, Epidermal Growth Factor ; Receptors, Cell Surface/*genetics ; Sequence Homology, Nucleic Acid ; Transcription, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 38
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    Human T-cell receptor alpha-chain genes: location on chromosome 14 (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-04-05
    Beschreibung: The genes encoding the alpha chain of the human T-cell receptor have been mapped to chromosome 14, the chromosome on which the human immunoglobulin heavy chain locus resides. Thus, genes encoding two different classes of antigen receptor are present on the same chromosome. Furthermore, breaks involving chromosome 14 are frequently seen in tumors of T-cell origin. The potential relation of these chromosome abnormalities to alpha-chain genes is discussed.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jones, C -- Morse, H G -- Kao, F T -- Carbone, A -- Palmer, E -- CA-18734/CA/NCI NIH HHS/ -- HD-02080/HD/NICHD NIH HHS/ -- HD-17717/HD/NICHD NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1985 Apr 5;228(4695):83-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3919444" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Chromosome Aberrations ; Chromosome Disorders ; *Chromosome Mapping ; *Chromosomes, Human, 13-15 ; Cricetinae ; Cricetulus ; DNA/genetics ; Humans ; Immunoglobulin Heavy Chains/*genetics ; Immunoglobulin alpha-Chains/*genetics ; Leukemia/genetics ; Lymphoma/genetics ; Nucleic Acid Hybridization ; Receptors, Antigen, T-Cell/*genetics
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 39
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    Partial primary structure of the alpha and beta chains of human tumor T-cell receptors (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-18
    Beschreibung: The T-cell receptor for antigen (Ti) was purified from the human tumor cell line HPB-ALL. Amino-terminal sequence analysis of an acid-cleaved peptide of the Ti alpha chain showed that it is highly homologous to a putative murine alpha chain recently described. Amino-terminal sequence analysis of the Ti beta chain revealed that it shares 50 percent homology with the Ti beta chain amino acid sequences from two other human T-cell tumors. Nucleotide sequence analysis of a complementary DNA clone encoding the Ti beta chain from the HPB-MLT cell line showed that this chain represents a second human constant region gene segment and suggested that it arises from direct joining of the variable and joining gene segments without any intervening D region sequences.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jones, N -- Leiden, J -- Dialynas, D -- Fraser, J -- Clabby, M -- Kishimoto, T -- Strominger, J L -- Andrews, D -- Lane, W -- Woody, J -- 5 R01 AI15669/AI/NIAID NIH HHS/ -- AI10736/AI/NIAID NIH HHS/ -- Y001CP00502/CP/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Jan 18;227(4684):311-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3871253" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Animals ; Base Sequence ; Cell Line ; Humans ; Immunoglobulin Constant Regions/genetics ; Leukemia, Lymphoid/immunology ; Lymphoma/immunology ; Mice ; Nucleic Acid Hybridization ; Receptors, Antigen, T-Cell/*genetics ; T-Lymphocytes/*immunology
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 40
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    Control of cytochrome P1-450 gene expression by dioxin (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-03-22
    Beschreibung: The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) may produce its effects by altering gene expression in susceptible cells. In mouse hepatoma cells, TCDD induces the transcription of the cytochrome P1-450 gene, whose product, aryl hydrocarbon hydroxylase, contributes both to the detoxification and to the metabolic activation of carcinogenic polycyclic aromatic hydrocarbons. A DNA fragment containing sequences flanking the 5' end of the cytochrome P1-450 gene was isolated and analyzed. This DNA fragment contains a cis-acting control element with at least three functional domains: a putative promoter, an inhibitory domain upstream from the promoter that blocks its function, and a TCDD-responsive domain still farther (1265 to 1535 base pairs) upstream of the promoter. These findings, together with results from earlier studies, imply that transcription of the cytochrome P1-450 gene is under both positive and negative control by at least two trans-acting regulatory factors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jones, P B -- Galeazzi, D R -- Fisher, J M -- Whitlock, J P Jr -- CA09302/CA/NCI NIH HHS/ -- CA32786/CA/NCI NIH HHS/ -- GM07149/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Mar 22;227(4693):1499-502.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3856321" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acetyltransferases/biosynthesis/genetics ; Animals ; Cell Line ; Chloramphenicol O-Acetyltransferase ; Cytochrome P-450 Enzyme System/*genetics ; DNA, Recombinant ; Dioxins/*pharmacology ; Enzyme Induction ; Gene Expression Regulation/*drug effects ; *Genes, Regulator ; Liver Neoplasms, Experimental ; Mice ; *Promoter Regions, Genetic ; Tetrachlorodibenzodioxin/*pharmacology ; Transcription Factors/metabolism ; Transcription, Genetic/drug effects ; Transfection
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 41
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    Human dioxin-inducible cytochrome P1-450: complementary DNA and amino acid sequence (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-04-05
    Beschreibung: Induction of cytochrome P1-450 has been linked to susceptibility to certain chemically induced cancers in mouse and man. Treatment of the human cell line MCF-7 with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) results in high levels of aryl hydrocarbon (benzo[a]pyrene) hydroxylase (P1-450) activity. This cell line was used to isolate a human P1-450 full-length complementary DNA (cDNA) clone. The cDNA is 2566 nucleotides in length, encodes a polyadenylated messenger RNA (2.8 kilobases in length), and has a continuous reading frame producing a protein with 512 residues (molecular weight, 58,151). The human P1-450 cDNA and protein are 63 percent and 80 percent similar to mouse P1-450 cDNA and protein, respectively. Whereas the mouse TCDD-inducible P-450 gene subfamily has two members (P1-450 and P3-450), the human TCDD-inducible gene subfamily appears to have only one gene (P1-450).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jaiswal, A K -- Gonzalez, F J -- Nebert, D W -- New York, N.Y. -- Science. 1985 Apr 5;228(4695):80-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3838385" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Animals ; Base Sequence ; Carcinogens/pharmacology ; Cell Line ; Cricetinae ; Cytochrome P-450 Enzyme System/*genetics ; DNA/*genetics ; Dioxins/*pharmacology ; Enzyme Induction ; Humans ; Mice ; Nucleic Acid Hybridization ; Rabbits ; Tetrachlorodibenzodioxin/*pharmacology
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 42
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    Transfection of v-rasH DNA into MCF-7 human breast cancer cells bypasses dependence on estrogen for tumorigenicity (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-10
    Beschreibung: The natural history of estrogen-responsive breast cancers often involves a phenotypic change to an estrogen-unresponsive, more aggressive tumor. The human breast cancer cell line, MCF-7, which requires estradiol for tumor formation in vivo and shows growth stimulation in response to estradiol in vitro, is a model for hormone-responsive tumors. The v-rasH onc gene was transfected into MCF-7 cells. The cloned MCF-7ras transfectants, which expressed the v-rasH messenger RNA and v-rasH p21 protein (21,000 daltons), were characterized. In contrast to the parental cell line, MCF-7ras cells no longer responded to exogenous estrogen in culture and their growth was minimally inhibited by exogenous antiestrogens. When tested in the nude mouse, the MCF-7ras cells were fully tumorigenic in the absence of estrogen supplementation. Thus, cells acquiring an activated onc gene can bypass the hormonal regulatory signals that trigger the neoplastic growth of a human breast cancer cell line.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kasid, A -- Lippman, M E -- Papageorge, A G -- Lowy, D R -- Gelmann, E P -- New York, N.Y. -- Science. 1985 May 10;228(4700):725-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/4039465" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Breast Neoplasms/chemically induced/*genetics ; Cell Line ; Cell Transformation, Neoplastic/*chemically induced ; DNA, Neoplasm/genetics ; Estrogens/*pharmacology ; Female ; Humans ; Mice ; Mice, Nude ; Neoplasms, Experimental/chemically induced/genetics ; *Oncogenes ; Pyrrolidines/pharmacology ; Repetitive Sequences, Nucleic Acid ; Thiophenes/pharmacology ; *Transfection
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 43
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    Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1) (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-10-18
    Beschreibung: Complementary DNA (cDNA) clones encoding human macrophage-specific specific colony-stimulating factor (CSF-1) were isolated. One cDNA clone codes for a mature polypeptide of 224 amino acids and a putative leader of 32 amino acids. This cDNA, which was cloned in the Okayama-Berg expression vector, specifies the synthesis of biologically active CSF-1 in COS cells, as determined by a specific radioreceptor assay, macrophage bone marrow colony formation, and antibody neutralization. Most of the cDNA isolates contain part of an intron sequence that changes the reading frame, resulting in an abrupt termination of translation; these cDNA's were inactive in COS cells. The CSF-1 appears to be encoded by a single-copy gene, but its expression results in the synthesis of several messenger RNA species, ranging in size from about 1.5 to 4.5 kilobases.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kawasaki, E S -- Ladner, M B -- Wang, A M -- Van Arsdell, J -- Warren, M K -- Coyne, M Y -- Schweickart, V L -- Lee, M T -- Wilson, K J -- Boosman, A -- C32551/PHS HHS/ -- New York, N.Y. -- Science. 1985 Oct 18;230(4723):291-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2996129" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Base Sequence ; Cell Line ; *Cloning, Molecular ; Colony-Stimulating Factors/*genetics ; DNA/*metabolism ; DNA Restriction Enzymes ; *Genes ; Humans ; Macrophages/*metabolism ; Pancreatic Neoplasms ; RNA, Messenger/genetics ; Transcription, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 44
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    Pathways of protein secretion in eukaryotes (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-10-04
    Beschreibung: Protein secretion from cells can take several forms. Secretion is constitutive if proteins are secreted as fast as they are synthesized. In regulated secretion newly synthesized proteins destined for secretion are stored at high concentration in secretory vesicles until the cell receives an appropriate stimulus. When both constitutive and regulated protein secretion can take place in the same cell a mechanism must exist for sorting the correct secretory protein into the correct secretory vesicle. The secretory vesicle must then be delivered to the appropriate region of plasma membrane. Transfection of DNA encoding foreign secretory proteins into regulated secretory cells has provided insight into the specificity of sorting into secretory vesicles.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kelly, R B -- New York, N.Y. -- Science. 1985 Oct 4;230(4721):25-32.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2994224" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adrenocorticotropic Hormone/secretion ; Animals ; Calcium/metabolism ; Calmodulin/metabolism ; Carrier Proteins/physiology ; Cell Compartmentation ; Cell Fusion ; Cell Line ; Cells/*secretion ; Cytoplasmic Granules/metabolism ; Eukaryotic Cells/cytology/*secretion ; Exocytosis ; Golgi Apparatus/ultrastructure ; Half-Life ; Leukemia Virus, Murine/genetics ; Lysosomes/enzymology ; Membrane Proteins/genetics/metabolism ; Microscopy, Electron ; Models, Biological ; Peptide Hydrolases/metabolism ; Pituitary Gland/cytology ; Pro-Opiomelanocortin/secretion ; Proteins/*secretion ; Transfection
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 45
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    Amplification of a novel v-erbB-related gene in a human mammary carcinoma (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-09-06
    Beschreibung: The cellular gene encoding the receptor for epidermal growth factor (EGF) has considerable homology to the oncogene of avian erythroblastosis virus. In a human mammary carcinoma, a DNA sequence was identified that is related to v-erbB but amplified in a manner that appeared to distinguish it from the gene for the EGF receptor. Molecular cloning of this DNA segment and nucleotide sequence analysis revealed the presence of two putative exons in a DNA segment whose predicted amino acid sequence was closely related to, but different from, the corresponding sequence of the erbB/EGF receptor. Moreover, this DNA segment identified a 5-kilobase transcript distinct from the transcripts of the EGF receptor gene. Thus, a new member of the tyrosine kinase proto-oncogene family has been identified on the basis of its amplification in a human mammary carcinoma.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉King, C R -- Kraus, M H -- Aaronson, S A -- New York, N.Y. -- Science. 1985 Sep 6;229(4717):974-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2992089" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Base Sequence ; Breast Neoplasms/*genetics ; Cell Line ; Cloning, Molecular ; DNA, Neoplasm/*genetics ; Female ; *Gene Amplification ; Gene Expression Regulation ; Humans ; *Oncogenes ; Protein Kinases/*genetics ; Protein-Tyrosine Kinases ; Receptor, Epidermal Growth Factor ; Receptors, Cell Surface/*genetics
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 46
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    Insertion mutagenesis of embryonal carcinoma cells by retroviruses (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-03
    Beschreibung: Mutagenesis was studied in cultured F9 embryonal carcinoma cells infected with a variant of Moloney murine leukemia virus. Proviral insertion induced the inactivation of the hypoxanthine phosphoribosyltransferase locus, and the virus was used to isolate the mutated genes rapidly. Mutagenesis by these methods may be useful for the genetic dissection of the various mammalian cell phenotypes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉King, W -- Patel, M D -- Lobel, L I -- Goff, S P -- Nguyen-Huu, M C -- New York, N.Y. -- Science. 1985 May 3;228(4699):554-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3838595" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Base Sequence ; Cell Line ; DNA/genetics ; DNA, Neoplasm/genetics ; DNA, Recombinant/metabolism ; DNA, Viral/genetics ; Mice ; Moloney murine leukemia virus/physiology ; *Mutation ; Nucleic Acid Hybridization ; Rats ; Retroviridae/*physiology ; Teratoma/*genetics/microbiology
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 47
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    Antibodies to peptides detect new hepatitis B antigen: serological correlation with hepatocellular carcinoma (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-25
    Beschreibung: The expression of a previously unidentified gene product, encoded by the hepatitis B virus (HBV) genome, has been achieved with a recombinant SV40 expression vector. Antibodies against synthetic peptides representing defined regions of this protein were used to screen cells infected with recombinant virus as well as tissues naturally infected with HBV. A 24,000-dalton protein (p24) was detected in cells infected with recombinant virus and a 28,000-dalton protein (p28) was detected in tissues infected with HBV. The peptides or recombinant-derived protein were used as antigens to screen sera from individuals infected with HBV. Specific antibodies were detected predominantly in sera from patients with hepatocellular carcinoma. The presence of p28 in tissues infected with HBV and the appearance of specific antibodies in infectious sera establish the existence of an additional marker for HBV infection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Moriarty, A M -- Alexander, H -- Lerner, R A -- Thornton, G B -- New York, N.Y. -- Science. 1985 Jan 25;227(4685):429-33.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2981434" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Carcinoma, Hepatocellular/diagnosis/*immunology ; Cell Line ; Cloning, Molecular ; Enzyme-Linked Immunosorbent Assay ; Genetic Vectors ; Hepatitis B/diagnosis/*immunology ; Hepatitis B Antibodies/*analysis/immunology ; Hepatitis B Antigens/*analysis/immunology ; Humans ; Liver/*immunology ; Liver Neoplasms/diagnosis/*immunology ; Molecular Weight ; Peptides/immunology ; Simian virus 40/genetics ; Viral Proteins/immunology
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 48
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    Genes for beta chain of human T-cell antigen receptor map to regions of chromosomal rearrangement in T cells (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-03
    Beschreibung: The T-cell antigen receptor is a cell-surface molecule that participates in the immune response. In the present experiments the genes encoding the beta chain of the T-cell receptor were found to reside on the long arm of human chromosome 7 at or near band q32. Related sequences were found on the short arm of chromosome 7 in bands p15-21 in some experiments. Chromosomal rearrangements in T-cells from normal individuals and patients with ataxia telangiectasia have previously been observed at and near these map assignments for the beta-chain genes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Morton, C C -- Duby, A D -- Eddy, R L -- Shows, T B -- Seidman, J G -- CA-07511/CA/NCI NIH HHS/ -- GM-20454/GM/NIGMS NIH HHS/ -- HD-05196/HD/NICHD NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1985 May 3;228(4699):582-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3983642" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Ataxia Telangiectasia/genetics ; Chromosome Aberrations/genetics ; Chromosome Disorders ; *Chromosome Mapping ; Chromosomes, Human, 6-12 and X ; DNA/genetics ; Genes ; Humans ; Hybrid Cells/metabolism ; Mice ; Nucleic Acid Hybridization ; Receptors, Antigen, T-Cell/*genetics
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 49
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    Enhanced transcription of c-myc in bursal lymphoma cells requires continuous protein synthesis (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-12-06
    Beschreibung: In several bursal lymphoma cell lines in which c-myc transcription is regulated by avian leukosis virus (ALV) long terminal repeat (LTR) sequences, protein synthesis inhibition decreases the transcriptional activity of c-myc as well as other LTR driven viral genes. This decrease in transcription is associated with a change in the chromatin structure of c-myc, as measured by deoxyribonuclease I (DNase I) hypersensitivity, and a shift of transcription from the LTR to the normal c-myc promoter. In contrast, cycloheximide had little or no effect on the transcription of LTR driven genes in infected chicken embryo fibroblasts treated with the drug. These results suggest that a labile, cell type-specific protein may interact with the retroviral LTR and regulate transcription of genes under LTR control. Further, the results demonstrate that the increase in intracellular concentration of c-myc RNA induced by cycloheximide treatment of normal cells is the result of stabilization of this message.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Linial, M -- Gunderson, N -- Groudine, M -- CA 18282/CA/NCI NIH HHS/ -- CA 28151/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Dec 6;230(4730):1126-32.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2999973" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Avian Leukosis/genetics ; Avian Leukosis Virus ; Bursa of Fabricius/cytology ; Cell Line ; Chick Embryo ; Chickens ; Chromatin/drug effects ; Cycloheximide/pharmacology ; Dactinomycin/pharmacology ; Emetine/pharmacology ; Lymphoma/*genetics ; Neoplasm Proteins/*biosynthesis ; *Oncogenes ; RNA, Neoplasm/metabolism ; Repetitive Sequences, Nucleic Acid ; *Transcription, Genetic/drug effects
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 50
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    HTLV-III infection in brains of children and adults with AIDS encephalopathy (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-11
    Beschreibung: Unexplained debilitating dementia or encephalopathy occurs frequently in adults and children with the acquired immune deficiency syndrome (AIDS). Brains from 15 individuals with AIDS and encephalopathy were examined by Southern analysis and in situ hybridization for the presence of human T-cell leukemia (lymphotropic) virus type III (HTLV-III), the virus believed to be the causative agent of AIDS. HTLV-III DNA was detected in the brains of five patients, and viral-specific RNA was detected in four of these. In view of these findings and the recent demonstration of morphologic and genetic relatedness between HTLV-III and visna virus, a lentivirus that causes a chronic degenerative neurologic disease in sheep, HTLV-III should be evaluated further as a possible cause of AIDS encephalopathy.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shaw, G M -- Harper, M E -- Hahn, B H -- Epstein, L G -- Gajdusek, D C -- Price, R W -- Navia, B A -- Petito, C K -- O'Hara, C J -- Groopman, J E -- New York, N.Y. -- Science. 1985 Jan 11;227(4683):177-82.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2981429" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acquired Immunodeficiency Syndrome/microbiology ; Adult ; Antibodies, Viral/analysis ; Brain Diseases/*microbiology ; Cerebral Cortex/analysis/*microbiology ; Child ; Deltaretrovirus/*isolation & purification ; Dementia/microbiology ; Female ; Humans ; Infant ; Male ; Middle Aged ; Nucleic Acid Hybridization ; RNA, Viral/analysis
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 51
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    Molecular cloning of the complementary DNA for human tumor necrosis factor (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-04-12
    Beschreibung: Tumor necrosis factor (TNF) is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF was purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and showed cytotoxic and cytostatic activities against various human tumor cell lines. The amino acid sequence was determined for the amino terminal end of the purified protein, and oligodeoxyribonucleotide probes were synthesized on the basis of this sequence. Complementary DNA (cDNA) encoding human TNF was cloned from induced HL-60 messenger RNA and was confirmed by hybrid-selection assay, direct expression in COS-7 cells, and nucleotide sequence analysis. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. Expression of high levels of human TNF in Escherichia coli was accomplished under control of the bacteriophage lambda PL promoter and gene N ribosome binding site.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wang, A M -- Creasey, A A -- Ladner, M B -- Lin, L S -- Strickler, J -- Van Arsdell, J N -- Yamamoto, R -- Mark, D F -- New York, N.Y. -- Science. 1985 Apr 12;228(4696):149-54.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3856324" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Animals ; Base Sequence ; Cell Line ; Cells, Cultured ; *Cloning, Molecular ; DNA/*genetics ; DNA, Recombinant/metabolism ; Glycoproteins/*genetics/isolation & purification/pharmacology ; Humans ; Leukemia, Myeloid/metabolism ; Mice ; Mice, Nude ; Neoplasms, Experimental/drug therapy ; Nucleic Acid Hybridization ; RNA, Messenger/genetics ; Rabbits ; Rats ; Tumor Necrosis Factor-alpha ; Xenopus
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 52
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    Bovine leukemia virus long terminal repeat: a cell type-specific promoter (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-18
    Beschreibung: The functional activity of the promoter unit contained within the long terminal repeat (LTR) of bovine leukemia virus (BLV) was examined by monitoring transient expression of a heterologous gene placed under its control. Various cell lines were transfected with recombinant plasmids carrying the bacterial chloramphenicol acetyltransferase (CAT) gene coupled to the BLV LTR (pBL-cat). Transient expression of CAT activity directed by the BLV LTR was observed only in the established BLV-producer cell lines derived from fetal lamb kidney (FLK) cells and bat lung cells. The amount of CAT activity transiently expressed in FLK-BLV cells was decreased approximately tenfold by deletion of LTR sequences located within a region 100 to 170 nucleotides upstream of the RNA start site. Surprisingly, removal of the region 50 base pairs downstream of the RNA initiation site to the 3'-end of the LTR reduced the expression of CAT activity by 87 percent. The BLV LTR thus appears to be an unusual promoter unit, functioning in a cell type-specific manner and possessing sequences on both the 5' and 3' sides of the RNA start site that influence gene expression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Derse, D -- Caradonna, S J -- Casey, J W -- CA07392/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Jan 18;227(4684):317-20.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2981431" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cattle ; Cell Line ; Chiroptera ; DNA, Recombinant/metabolism ; Deltaretrovirus/genetics ; Genes, Regulator ; Haplorhini ; Humans ; Leukemia Virus, Bovine/*genetics ; *Promoter Regions, Genetic ; RNA, Viral/*genetics ; *Repetitive Sequences, Nucleic Acid ; Retroviridae/*genetics ; Sheep ; Transcription, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 53
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    A transgenic mouse model of the chronic hepatitis B surface antigen carrier state (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-12-06
    Beschreibung: In an attempt to establish a model of the healthy carrier state in hepatitis B virus (HBV) infections, transgenic mice expressing HBV genes were produced. Fertilized one-cell eggs were microinjected with subgenomic fragments of HBV DNA containing the coding regions for the HBV surface antigen (HBsAg) and pre-S and X antigens. Either the normal (HBV) or metallothionein promoters were used to obtain expression of the HBV genes. There was no evidence of viral replication or tissue pathology. The integrated HBV DNA sequences were inherited in a normal Mendelian fashion. Three of 16 transgenic mice expressed HBV-encoded gene products to which they were immunologically tolerant. Expression was not tissue specific and may be influenced by the genomic integration site and cellular factors. Both HBsAg and pre-S antigen were detectable within the cytoplasm of hepatocytes and renal tubular epithelial cells. High serum concentrations of HBsAg were detectable and the secreted product appeared authentic as judged by mean density, morphology, mean particle diameter, polypeptide composition, and antigenicity. The absence of tissue pathology in these immunologically tolerant animals supports the hypothesis that cellular injury under these conditions is not a direct consequence of expression of the pre-S or HBs regions of the HBV genome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chisari, F V -- Pinkert, C A -- Milich, D R -- Filippi, P -- McLachlan, A -- Palmiter, R D -- Brinster, R L -- AI00585/AI/NIAID NIH HHS/ -- AI20001/AI/NIAID NIH HHS/ -- AI20720/AI/NIAID NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1985 Dec 6;230(4730):1157-60.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3865369" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Carrier State/*genetics/immunology ; *Disease Models, Animal ; *Genetic Engineering ; Hepatitis B/*genetics/immunology ; Hepatitis B Surface Antigens/*genetics ; Hepatitis B virus/genetics ; Humans ; Liver/microbiology ; Mice ; Mice, Inbred C57BL/genetics ; Nucleic Acid Hybridization
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 54
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    Individual tumors of multifocal EB virus-induced malignant lymphomas in tamarins arise from different B-cell clones (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-10
    Beschreibung: Cotton-top tamarins were inoculated with sufficient Epstein-Barr virus to induce multiple tumors in each animal within 14 to 21 days. The tumors consisted of large-cell lymphomas that contained multiple copies of the Epstein-Barr virus genome and generated Epstein-Barr virus-carrying cell lines showing no detectable consistent chromosomal abnormality. Hybridization of tumor DNA with immunoglobulin gene probes revealed that each lymphoma was oligo- or monoclonal in origin and that individual tumors from the same animal arose from different B-cell clones. Thus the virus induced multiple transformation events in tamarins in vivo to cause malignant tumors resembling the Epstein-Barr virus-associated lymphomas of patients with organ transplants.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cleary, M L -- Epstein, M A -- Finerty, S -- Dorfman, R F -- Bornkamm, G W -- Kirkwood, J K -- Morgan, A J -- Sklar, J -- CA 34233/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 May 10;228(4700):722-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2986287" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; B-Lymphocytes/*microbiology ; Burkitt Lymphoma/genetics/*microbiology ; Cell Line ; DNA, Neoplasm/genetics ; Heart Transplantation ; Herpesvirus 4, Human ; Humans ; Neoplasms, Experimental/genetics/microbiology ; Nucleic Acid Hybridization ; Saguinus
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 55
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    Expression of a microinjected porcine class I major histocompatibility complex gene in transgenic mice (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-03
    Beschreibung: A porcine class I major histocompatibility complex (SLA) gene has been introduced into the genome of a C57BL/10 mouse. This transgenic mouse expressed SLA antigen on its cell surfaces and transmitted the gene to offspring, in which the gene is also expressed. Skin grafts of such transgenic mice were rejected by normal C57BL/10 mice, suggesting that the foreign SLA antigen expressed in the transgenic mice is recognized as a functional transplantation antigen.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Frels, W I -- Bluestone, J A -- Hodes, R J -- Capecchi, M R -- Singer, D S -- GM 07825/GM/NIGMS NIH HHS/ -- GM 2116B/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 May 3;228(4699):577-80.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3885396" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; DNA/genetics ; Female ; Genes ; Genetic Engineering ; Graft Rejection ; H-2 Antigens/genetics ; *Major Histocompatibility Complex ; Male ; Mice ; Mice, Inbred C57BL/genetics ; Microinjections ; Nucleic Acid Hybridization ; Skin Transplantation ; Swine
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 56
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    Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-12-06
    Beschreibung: A novel potential cell surface receptor of the tyrosine kinase gene family has been identified and characterized by molecular cloning. Its primary sequence is very similar to that of the human epidermal growth factor receptor and the v-erbB oncogene product; the chromosomal location of the gene for this protein is coincident with the neu oncogene, which suggests that the two genes may be identical.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Coussens, L -- Yang-Feng, T L -- Liao, Y C -- Chen, E -- Gray, A -- McGrath, J -- Seeburg, P H -- Libermann, T A -- Schlessinger, J -- Francke, U -- New York, N.Y. -- Science. 1985 Dec 6;230(4730):1132-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2999974" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Base Sequence ; *Chromosome Mapping ; Chromosomes, Human, 16-18 ; Chromosomes, Human, 6-12 and X ; DNA/genetics ; Fetus/metabolism ; Humans ; Nucleic Acid Hybridization ; *Oncogenes ; Rats ; Receptor, Epidermal Growth Factor ; Receptors, Cell Surface/*genetics
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 57
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    Selective loss of a family of gene transcripts in a hereditary murine cataract (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-04
    Beschreibung: The eye lens of the Fraser mouse contains a dominantly inherited cataract with reduced amounts of seven distinct but homologous gamma crystallins encoded by a family of gamma-crystallin genes. The results of experiments with cultured lenses, cell-free RNA translation, and Northern blot hybridization indicated a specific loss of the family of gamma-crystallin messenger RNA's in the Fraser mouse lens. Southern blot hybridization of genomic DNA's from normal and Fraser mice showed no differences in gamma-crystallin coding sequences.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Garber, A T -- Winkler, C -- Shinohara, T -- King, C R -- Inana, G -- Piatigorsky, J -- Gold, R J -- New York, N.Y. -- Science. 1985 Jan 4;227(4682):74-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3964960" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cataract/*genetics ; Crystallins/*genetics ; Genes ; Lens, Crystalline/metabolism ; Mice ; Mice, Mutant Strains ; Nucleic Acid Hybridization ; Protein Biosynthesis ; RNA, Messenger/genetics
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 58
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    Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-09-20
    Beschreibung: A complementary DNA clone encoding the alpha subunit of the adenylate cyclase stimulatory G protein (Gs) was isolated and identified. A bovine brain complementary DNA library was screened with an oligonucleotide probe derived from amino acid sequence common to known G proteins. The only clone that was obtained with this probe has a complementary DNA insert of approximately 1670 base pairs. An antibody to a peptide synthesized according to deduced amino acid sequence reacts specifically with the alpha subunit of Gs. In addition, RNA that hybridizes with probes made from the clone is detected in wild-type S49 cells; however, cyc- S49 cells, which are deficient in Gs alpha activity, are devoid of this messenger RNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Harris, B A -- Robishaw, J D -- Mumby, S M -- Gilman, A G -- GM09731/GM/NIGMS NIH HHS/ -- GM34497/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Sep 20;229(4719):1274-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3839937" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adenylyl Cyclases/*metabolism ; Amino Acid Sequence ; Animals ; Base Sequence ; Cattle ; Cerebral Cortex ; *Cloning, Molecular ; DNA/*analysis ; Enzyme Activation ; Nerve Tissue Proteins/*genetics ; Nucleic Acid Hybridization ; Protein Biosynthesis ; Retina
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 59
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    Gene transfer and expression of human phenylalanine hydroxylase (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-04-05
    Beschreibung: Phenylketonuria (PKU) is caused by a genetic deficiency of the enzyme phenylalanine hydroxylase (PAH). A full-length complementary DNA clone of human PAH was inserted into a eukaryotic expression vector and transferred into mouse NIH3T3 cells which do not normally express PAH. The transformed mouse cells expressed PAH messenger RNA, immunoreactive protein, and enzymatic activity that are characteristic of the normal human liver products, demonstrating that a single gene contains all of the necessary genetic information to code for functional PAH. These results support the use of the human PAH probe in prenatal diagnosis and detection of carriers, to provide new opportunities for the biochemical characterization of normal and mutant enzymes, and in the investigation of alternative genetic therapies for PKU.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ledley, F D -- Grenett, H E -- DiLella, A G -- Kwok, S C -- Woo, S L -- HD-06495/HD/NICHD NIH HHS/ -- HD-17711/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1985 Apr 5;228(4695):77-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3856322" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cell Line ; Cloning, Molecular ; DNA, Recombinant/metabolism ; *Genetic Engineering ; Humans ; Mice ; Nucleic Acid Hybridization ; Phenylalanine Hydroxylase/*genetics ; Phenylketonurias/diagnosis/genetics ; Prenatal Diagnosis ; Rats
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 60
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    Evidence that the v-sis gene product transforms by interaction with the receptor for platelet-derived growth factor (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-10-18
    Beschreibung: A scheme for partial purification of biologically active v-sis-coded protein from cells transformed with simian sarcoma virus (SSV) has made possible a functional comparison of the transforming protein with platelet-derived growth factor (PDGF). The SSV-transforming gene product is capable of specifically binding PDGF receptors, stimulating tyrosine phosphorylation of PDGF receptors, and inducing DNA synthesis in quiescent fibroblasts. Each of these activities was specifically inhibited by antibodies to different regions of the v-sis gene product. Moreover, viral infection of a variety of cell types revealed a strict correlation between those cells possessing PDGF receptors and those susceptible to transformation by SSV. These findings provide evidence that SSV-transforming activity is mediated by the interaction of a virus-coded mitogen with PDGF receptors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Leal, F -- Williams, L T -- Robbins, K C -- Aaronson, S A -- New York, N.Y. -- Science. 1985 Oct 18;230(4723):327-30.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2996133" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Aorta/metabolism ; Cattle ; Cell Line ; *Cell Transformation, Viral ; Cells, Cultured ; Fibroblasts/metabolism ; *Genes ; *Genes, Viral ; Humans ; Kinetics ; Mink ; Molecular Weight ; Muscle, Smooth/metabolism ; Muscle, Smooth, Vascular/metabolism ; Platelet-Derived Growth Factor/*metabolism ; Receptors, Cell Surface/isolation & purification/*metabolism ; Receptors, Platelet-Derived Growth Factor ; Retroviridae/*genetics ; Sarcoma Virus, Woolly Monkey/*genetics ; Viral Proteins/genetics/*metabolism
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 61
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    Localization of the gene encoding the human interleukin-2 receptor on chromosome 10 (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-06-28
    Beschreibung: The human interleukin-2 receptor is an inducible growth factor receptor present on the surface of activated T lymphocytes. The receptor is required for a normal T-cell immune response. High-resolution fluorescence-activated chromosome sorting and DNA spot-blot analysis with complementary DNA's for the interleukin-2 receptor indicated that the receptor gene was located on chromosome 9, 10, 11, or 12. In situ hybridization studies showed that the interleukin-2 receptor gene is on the short arm of chromosome 10, p14----15.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Leonard, W J -- Donlon, T A -- Lebo, R V -- Greene, W C -- New York, N.Y. -- Science. 1985 Jun 28;228(4707):1547-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3925551" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Cell Line ; *Chromosomes, Human, 6-12 and X ; DNA/analysis ; Humans ; Lymphocyte Activation ; Male ; Nucleic Acid Hybridization ; Phytohemagglutinins/pharmacology ; Receptors, Immunologic/*genetics ; Receptors, Interleukin-2 ; T-Lymphocytes/analysis/immunology
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 62
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    Molecular cloning of a gene sequence regulated by nerve growth factor (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-07-26
    Beschreibung: Nerve growth factor (NGF) is essential for the development and differentiation of sympathetic or sensory neurons. A complementary DNA was cloned that corresponds to a gene sequence induced more than 50-fold in a cultured target cell line of pheochromocytoma cells (PC12 cells) 5 hours after the addition of NGF. The induced messenger RNA encodes a 90,000-dalton polypeptide that may represent one of the primary events in NGF-induced differentiation of neurons.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Levi, A -- Eldridge, J D -- Paterson, B M -- New York, N.Y. -- Science. 1985 Jul 26;229(4711):393-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3839317" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Actins/genetics ; Adrenal Gland Neoplasms/genetics ; Animals ; Base Sequence ; Cell Line ; Chickens ; *Cloning, Molecular ; DNA/genetics ; Gene Expression Regulation ; *Genes ; Nerve Growth Factors/*physiology ; Nucleic Acid Hybridization ; Pheochromocytoma/genetics ; RNA, Messenger/genetics ; Rabbits ; Rats
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 63
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    Transcription of novel open reading frames of AIDS retrovirus during infection of lymphocytes (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-09-27
    Beschreibung: The retrovirus frequently isolated from patients with the acquired immune deficiency syndrome (AIDS) has two novel open reading frames previously designated "A" and "B." The "A" region was found to be specifically expressed as polyadenylated RNA's of 5.5 and 5.0 kilobases in infected cells. The "B" region was expressed as 1.8- to 2.0-kilobase RNA species. Additional full-length and spliced messenger RNA's of the env region were also identified.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rabson, A B -- Daugherty, D F -- Venkatesan, S -- Boulukos, K E -- Benn, S I -- Folks, T M -- Feorino, P -- Martin, M A -- New York, N.Y. -- Science. 1985 Sep 27;229(4720):1388-90.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2994220" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acquired Immunodeficiency Syndrome/*microbiology ; DNA, Viral/genetics ; Deltaretrovirus/*genetics ; Genes, Viral ; Humans ; Lymphocytes/*microbiology ; Nucleic Acid Hybridization ; RNA, Messenger/genetics ; RNA, Viral/genetics ; Repetitive Sequences, Nucleic Acid ; *Transcription, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 64
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    Chromosomal locations of human tissue plasminogen activator and urokinase genes (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-11-08
    Beschreibung: A panel of human-mouse somatic cell hybrids and specific complementary DNA probes were used to map the human tissue plasminogen activator and urokinase genes to human chromosomes 8 and 10, respectively. This result is in contrast to a previous assignment of a plasminogen activator gene to chromosome 6. As neoplastic cells produce high levels of plasminogen activator, it is of interest that aberrations of chromosome 8 have been linked to various leukemias and lymphomas and that two human oncogenes, c-mos and c-myc, have also been mapped to chromosome 8.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rajput, B -- Degen, S F -- Reich, E -- Waller, E K -- Axelrod, J -- Eddy, R L -- Shows, T B -- GM20454/GM/NIGMS NIH HHS/ -- HD05196/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1985 Nov 8;230(4726):672-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3840278" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; *Chromosome Mapping ; Chromosomes, Human, 6-12 and X ; DNA/genetics ; Genes ; Humans ; Hybrid Cells ; Leukemia/genetics ; Lymphoma/genetics ; Mice ; Nucleic Acid Hybridization ; Oncogenes ; Plasminogen Activators/*genetics ; Urokinase-Type Plasminogen Activator/*genetics
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 65
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    Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-11-29
    Beschreibung: In situ hybridization was used to detect human cytomegalovirus (HCMV) in the peripheral blood mononuclear cells of some naturally infected (seropositive) individuals. A subpopulation of cells hybridized specifically to a portion of the HCMV genome that is heavily transcribed during the immediate-early period of infection. The hybridization signal was markedly reduced by base hydrolysis and ribonuclease, and therefore the probe appears to be detecting viral RNA. A fluorescence-activated cell sorter was used to select lymphocytes bearing the OKT4 and OKT8 markers. Hybridization with the HCMV probe revealed a higher proportion of positive cells in the OKT4 than in the OKT8 subset. This observation specifically identifies lymphocytes as a cell population involved in natural HCMV infection and suggests that lymphocytes may be a reservoir for maintaining infection and may also serve as a vehicle for its spread by blood transfusion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Schrier, R D -- Nelson, J A -- Oldstone, M B -- AI-07007/AI/NIAID NIH HHS/ -- AI-21640/AI/NIAID NIH HHS/ -- CA-35048/CA/NCI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1985 Nov 29;230(4729):1048-51.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2997930" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Antigens, Differentiation, T-Lymphocyte ; Antigens, Surface/analysis ; Cytomegalovirus/*isolation & purification ; Cytomegalovirus Infections/*microbiology ; Genes, Viral ; Humans ; Lymphocytes/immunology/*microbiology ; Nucleic Acid Hybridization ; RNA, Viral/analysis ; Virus Replication
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 66
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    Location of the c-yes gene on the human chromosome and its expression in various tissues (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-03-01
    Beschreibung: Analysis of DNA from human embryo fibroblasts showed that ten Eco RI fragments were hybridizable with the Yamaguchi sarcoma virus oncogene (v-yes). Four of the Eco RI fragments were assigned to chromosome 18 and one to chromosome 6. There was evidence for multiple copies of yes-related genes in the human genome; however, only a single RNA species, 4.8 kilobases in length, was related to yes in various cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Semba, K -- Yamanashi, Y -- Nishizawa, M -- Sukegawa, J -- Yoshida, M -- Sasaki, M -- Yamamoto, T -- Toyoshima, K -- New York, N.Y. -- Science. 1985 Mar 1;227(4690):1038-40.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2983418" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Avian Sarcoma Viruses/genetics ; Base Sequence ; *Chromosome Mapping ; Chromosomes, Human, 16-18 ; Chromosomes, Human, 6-12 and X ; DNA/genetics ; Humans ; Hybrid Cells/metabolism ; Mice ; Nucleic Acid Hybridization ; *Oncogenes ; Transduction, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 67
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    Deletion in chromosome 11p associated with a hepatitis B integration site in hepatocellular carcinoma (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-10-18
    Beschreibung: Hepatitis B virus (HBV), a virus with known carcinogenic potential, integrates into cellular DNA during long-term persistent infection in man. Hepatocellular carcinomas isolated from viral carriers often contain clonally propagated viral DNA integrations. As small chromosomal deletions are associated with several types of carcinomas, the occurrence of chromosomal deletions in association with HBV integration in hepatocellular carcinoma was studied. HBV integration was accompanied by a deletion of at least 13.5 kilobases of cellular sequences in a human hepatocellular carcinoma. The viral DNA integration and deletion of cellular sequences occurred on the short arm of chromosome 11 at location 11p13-11p14. The cellular sequences that were deleted at the site of HBV integration were lost from the tumor cells, leaving only a single copy of the remaining cellular allele.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rogler, C E -- Sherman, M -- Su, C Y -- Shafritz, D A -- Summers, J -- Shows, T B -- Henderson, A -- Kew, M -- AM17702/AM/NIADDK NIH HHS/ -- CA32605/CA/NCI NIH HHS/ -- CA37232-02/CA/NCI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1985 Oct 18;230(4723):319-22.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2996131" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Base Sequence ; Carcinoma, Hepatocellular/*genetics/microbiology ; *Chromosome Deletion ; *Chromosomes, Human, 6-12 and X ; Cloning, Molecular ; DNA Restriction Enzymes ; DNA, Viral/genetics ; Hepatitis B virus/*genetics ; Humans ; Hybrid Cells/cytology ; Liver Neoplasms/*genetics/microbiology ; Mice ; Nucleic Acid Hybridization
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 68
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    New sickle cell test (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-12-20
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marx, J L -- New York, N.Y. -- Science. 1985 Dec 20;230(4732):1365.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2999982" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Anemia, Sickle Cell/*diagnosis/genetics ; DNA Restriction Enzymes ; DNA-Directed DNA Polymerase ; Female ; Gene Amplification ; Genes ; Globins/genetics ; Humans ; Nucleic Acid Hybridization ; Pregnancy ; Prenatal Diagnosis
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 69
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    Involvement of the bcl-2 gene in human follicular lymphoma (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-06-21
    Beschreibung: Recombinant DNA probes were cloned for the areas flanking the breakpoint on chromosome 18 in cells from a patient with acute lymphocytic leukemia of the B-cell type; cells of this line carry the t(14;18) chromosomal translocation. Two of the probes detected DNA rearrangements in approximately 60 percent of the cases of follicular lymphoma screened. In follicular lymphoma, most of the breakpoints in band q21 of chromosome 18 were clustered within a short stretch of DNA, approximately 2.1 kilobases in length. Chromosome 18-specific DNA probes for the areas flanking the breakpoints also detected RNA transcripts 6 kilobases in length in various cell types. The gene coding for these transcript (the bcl-2 gene) seems to be interrupted in most cases of follicular lymphomas carrying the t(14;18) chromosomal translocation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tsujimoto, Y -- Cossman, J -- Jaffe, E -- Croce, C M -- CA16685/CA/NCI NIH HHS/ -- CA36521/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Jun 21;228(4706):1440-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3874430" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): B-Lymphocytes/ultrastructure ; Cell Line ; *Chromosomes, Human, 16-18 ; Cloning, Molecular ; Humans ; Leukemia, Lymphoid/genetics ; Lymphoma/*genetics ; *Oncogenes ; *Translocation, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 70
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    Prooxidant states and tumor promotion (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-25
    Beschreibung: There is convincing evidence that cellular prooxidant states--that is, increased concentrations of active oxygen and organic peroxides and radicals--can promote initiated cells to neoplastic growth. Prooxidant states can be caused by different classes of agents, including hyperbaric oxygen, radiation, xenobiotic metabolites and Fenton-type reagents, modulators of the cytochrome P-450 electron-transport chain, peroxisome proliferators, inhibitors of the antioxidant defense, and membrane-active agents. Many of these agents are promoters or complete carcinogens. They cause chromosomal damage by indirect action, but the role of this damage in carcinogenesis remains unclear. Prooxidant states can be prevented or suppressed by the enzymes of the cellular antioxidant defense and low molecular weight scavenger molecules, and many antioxidants are antipromoters and anticarcinogens. Finally, prooxidant states may modulate the expression of a family of prooxidant genes, which are related to cell growth and differentiation, by inducing alterations in DNA structure or by epigenetic mechanisms, for example, by polyadenosine diphosphate-ribosylation of chromosomal proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cerutti, P A -- New York, N.Y. -- Science. 1985 Jan 25;227(4685):375-81.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2981433" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antioxidants/pharmacology ; *Carcinogens/metabolism/pharmacology ; Cations/metabolism ; Cell Differentiation ; Cell Division ; Cell Line ; Cell Membrane/physiology ; *Cell Transformation, Neoplastic ; Chromosome Aberrations ; Chromosomes/drug effects ; Cytochrome P-450 Enzyme System/metabolism ; DNA/metabolism ; Electron Transport ; Gene Expression Regulation ; Humans ; Hydrogen Peroxide/metabolism ; Hydroxides/metabolism ; Hydroxyl Radical ; Lipid Peroxides/metabolism ; Microbodies/metabolism ; Mutation ; Neoplasms/*chemically induced ; Oxidation-Reduction ; Oxygen/*metabolism/physiology ; Poly Adenosine Diphosphate Ribose/metabolism ; Singlet Oxygen ; Sulfhydryl Compounds/physiology ; Superoxides/metabolism ; Ultraviolet Rays
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 71
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    Growth regulation of human melanocytes: mitogenic factors in extracts of melanoma, astrocytoma, and fibroblast cell lines (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-09-06
    Beschreibung: Melanocytes derived from fetal or adult skin do not propagate in vitro unless cultured in the presence of factors such as 12-O-tetradecanoylphorbol 13-acetate (TPA). In a search for physiological factors regulating the growth of melanocytes, extracts of various cultured cell types were tested. Factors produced by melanoma and astrocytoma cell lines support continued proliferation of melanocytes in the absence of TPA. WI-38, a fibroblast cell line derived from human embryonic lung, was the most active source of melanocyte growth factors. No melanocyte growth-promoting activity was found in extracts of cultured neuroblastoma, renal cancer, normal keratinocytes, or renal epithelium. Nerve growth factor, epidermal growth factor, melanocyte-stimulating hormone, transforming growth factor-beta, and platelet-derived growth factor did not have growth-promoting activity for melanocytes. The presence of melanocyte growth factors and TPA together resulted in the strongest mitogenic activity for melanocytes, permitting the recovery (at 20 days) of 4 to 20 times as many cells as in growth factor or TPA alone.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Eisinger, M -- Marko, O -- Ogata, S -- Old, L J -- 1R01 CA-32152/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Sep 6;229(4717):984-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/4023718" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Astrocytoma/physiopathology ; Cell Division/drug effects ; Cell Line ; Cholera Toxin/pharmacology ; Fibroblasts/physiology ; Growth Substances/isolation & purification/*pharmacology ; Humans ; Melanocytes/*cytology ; Melanoma/physiopathology ; Tetradecanoylphorbol Acetate/pharmacology
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 72
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    B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-11
    Beschreibung: The mouse heavy chain immunoglobulin gene contains a tissue-specific enhancer. The enhancer and flanking sequences were studied in vivo by carrying out dimethyl sulfate protection experiments on living cells, in combination with genomic sequencing. Relative to reactions on naked DNA, there are changes (protections and enhancements) in the reactivity of guanine residues to dimethyl sulfate within the enhancer sequence in myeloma, B, and early B cells, whereas virtually no alterations appear in cells of non-B lineage. Most of the affected residues are in four clusters, in sequences homologous to the octamer 5'CAGGTGGC 3' (C, cytosine; A, adenine; G. guanine; T, thymine). The alterations in the pattern of G reactivity are consistent with the tissue-specific binding of molecules to the mouse immunoglobulin heavy chain enhancer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ephrussi, A -- Church, G M -- Tonegawa, S -- Gilbert, W -- 2T 32 CA 09255/CA/NCI NIH HHS/ -- CA 14041/CA/NCI NIH HHS/ -- GM-09541-22/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Jan 11;227(4683):134-40.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3917574" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; B-Lymphocytes/*metabolism ; Base Sequence ; Cell Line ; *Enhancer Elements, Genetic ; *Genes, Regulator ; Guanine/metabolism ; Immunoglobulin Heavy Chains/*genetics ; Methylation ; Mice ; Multiple Myeloma/genetics ; RNA, Messenger/metabolism ; Sulfuric Acid Esters/metabolism
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 73
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    Cyanobacterial light-harvesting complex subunits encoded in two red light-induced transcripts (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-11-01
    Beschreibung: The major light-harvesting complex in cyanobacteria and red algae, the phycobilisome, is composed of chromophoric and nonchromophoric polypeptides. Two linked genes encoding major chromophoric components, the polypeptide subunits of phycocyanin, were isolated from the cyanobacterium Fremyella diplosiphon. Transcripts from this phycocyanin subunit gene cluster were present as major species in the cyanobacterium grown in red light, but not in cultures maintained in green light. The genes for the subunits of the red light-induced phycocyanin were transcribed together (beta-phycocyanin followed by alpha-phycocyanin) on two messenger RNA species; one contained 1600 bases while the other had 3800 bases. The latter, which encompassed the smaller transcript, contained additional sequences extending from the 3' end of the coding region of the alpha-phycocyanin gene. It may encode other light-induced components of the phycobilisome. Since phycocyanin, which effectively absorbs red light, becomes a dominant constituent of the phycobilisome in red light, these different levels may reflect an important adaptive mechanism of these organisms to their environment.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Conley, P B -- Lemaux, P G -- Grossman, A R -- GM 33436-01/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Nov 1;230(4725):550-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3931221" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Base Sequence ; Cyanobacteria/*genetics ; Light ; Nucleic Acid Hybridization ; Phycobilisomes ; Phycocyanin/genetics ; RNA, Messenger/metabolism ; *Transcription, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 74
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    Sequence homology and morphologic similarity of HTLV-III and visna virus, a pathogenic lentivirus (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-11
    Beschreibung: A study was conducted of the genetic relation between human T-cell lymphotropic retroviruses and visna virus. The human T-cell lymphotropic viruses include those associated with T-cell malignancies (HTLV-I and HTLV-II) as well as the etiologic agent of the acquired immune deficiency syndrome (HTLV-III). Visna virus, a slowly replicating and pathogenic but nononcogenic retrovirus of sheep, is a member of the subfamily Lentivirinae. Results obtained by molecular hybridization and heteroduplex analysis indicated that a greater extent of nucleotide sequence homology exists between HTLV-III and visna virus than between HTLV-III and any of the other viruses. The homology observed under conditions of low stringency spanned the entire genome, but was strongest in the gag/pol region. The morphogenesis and fine structure of HTLV-III and visna virus also demonstrated striking similarities. The data provide strong evidence for a close taxonomic and thus evolutionary relation between HTLV-III and the Lentivirinae subfamily.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gonda, M A -- Wong-Staal, F -- Gallo, R C -- Clements, J E -- Narayan, O -- Gilden, R V -- N01-CO-23910/CO/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Jan 11;227(4683):173-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2981428" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Base Sequence ; Deltaretrovirus/*genetics/ultrastructure ; Genes, Viral ; Microscopy, Electron ; Nucleic Acid Heteroduplexes ; Nucleic Acid Hybridization ; RNA, Viral ; Visna-maedi virus/*genetics/ultrastructure
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 75
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    A new role for DNA virus early proteins in viral carcinogenesis (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-04
    Beschreibung: The T antigen proteins encoded by DNA tumor virus early genes are involved in the transformation of normal cells to immortalized neoplastic cells that may or may not be tumorigenic in immunocompetent animals. Studies have been made of the tumorigenicity of DNA virus-transformed cells and the interactions of these cells in vivo and in vitro with immunologically nonspecific host effector cells such as natural killer cells and macrophages. The results imply that the T proteins determine the capacity of transformed cells to induce tumors by governing the level of susceptibility that transformed cells express to destruction by such host cellular defenses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lewis, A M Jr -- Cook, J L -- CA 31732/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Jan 4;227(4682):15-20.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3843807" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antigens, Polyomavirus Transforming ; Antigens, Viral, Tumor/*metabolism ; Cell Line ; Cell Transformation, Neoplastic/immunology/*metabolism ; *Cell Transformation, Viral ; Cricetinae ; Cytotoxicity, Immunologic ; DNA Viruses/*metabolism ; Humans ; Immunity, Cellular ; Killer Cells, Natural/immunology ; Major Histocompatibility Complex ; Mesocricetus ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Neoplasms, Experimental/immunology ; Rats ; Viral Proteins/*metabolism
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 76
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    Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-04-19
    Beschreibung: The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene from Escherichia coli has allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lobel, L I -- Patel, M -- King, W -- Nguyen-Huu, M C -- Goff, S P -- 2 P30 CA 23767/CA/NCI NIH HHS/ -- R01 CA 37176/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Apr 19;228(4697):329-32.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2984770" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Base Sequence ; DNA Transposable Elements ; DNA, Recombinant/metabolism ; DNA, Viral/genetics ; Escherichia coli/genetics ; *Genes, Bacterial ; *Genes, Viral ; Moloney murine leukemia virus/genetics ; Mutation ; Nucleic Acid Hybridization ; RNA, Transfer/*genetics ; Retroviridae/*genetics ; *Suppression, Genetic
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 77
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    Coupling of Tetrahymena ribosomal RNA splicing to beta-galactosidase expression in Escherichia coli (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-05-10
    Beschreibung: Splicing of the Tetrahymena ribosomal RNA precursor is mediated by the folded structure of the RNA molecule and therefore occurs in the absence of any protein in vitro. The Tetrahymena intervening sequence (IVS) has been inserted into the gene for the alpha-donor fragment of beta-galactosidase in a recombinant plasmid. Production of functional beta-galactosidase is dependent on RNA splicing in vivo in Escherichia coli. Thus RNA self-splicing can occur at a rate sufficient to support gene expression in a prokaryote, despite the likely presence of ribosomes on the nascent RNA. The beta-galactosidase messenger RNA splicing system provides a useful method for screening for splicing-defective mutations, several of which have been characterized.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Price, J V -- Cech, T R -- GM28039/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 May 10;228(4700):719-22.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2986286" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Base Sequence ; DNA Transposable Elements ; Escherichia coli/*genetics ; Galactosidases/*genetics ; *Genetic Engineering ; Nucleic Acid Hybridization ; Plasmids ; *RNA Splicing ; RNA, Messenger/genetics ; RNA, Ribosomal/*genetics ; Tetrahymena/*genetics ; beta-Galactosidase/biosynthesis/*genetics
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    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 78
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    A mammalian host-vector system that regulates expression and amplification of transfected genes by temperature induction (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-01-04
    Beschreibung: SV40-transformed simian cells that permit temperature-dependent regulation of vector DNA replication were isolated and characterized. These cell lines (ts COS cells) produce high levels of thermolabile large T antigen under the transcriptional control of the Rous sarcoma virus long terminal repeat. The ts COS cell lines can complement SV40 A gene mutants and support replication of SV40-origin containing vectors at 33 degrees C but not at 40 degrees C. It should now be possible to regulate the copy number of transfected plasmid DNA's and also maintain selectable vector sequences either as integrated DNA or as autonomously replicating episomes by modulating T antigen activity in ts COS cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rio, D C -- Clark, S G -- Tjian, R -- New York, N.Y. -- Science. 1985 Jan 4;227(4682):23-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2981116" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antigens, Viral, Tumor/genetics ; Cell Line ; Cell Transformation, Viral ; DNA Replication ; *Gene Amplification ; *Gene Expression Regulation ; *Genetic Vectors ; Haplorhini ; Plasmids ; RNA, Messenger/metabolism ; Simian virus 40/physiology ; Temperature ; *Transfection ; Virus Replication
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 79
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    A hydrophobic transmembrane segment at the carboxyl terminus of thy-1 (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-02-08
    Beschreibung: The mode of integration of the glycoprotein thy-1 within the cell membrane has been controversial due to an apparent lack of a transmembrane hydrophobic segment. Rat and mouse complementary DNA and genomic clones encoding the thy-1 molecule have been isolated and sequenced. These studies have enabled us to determine the intron-exon organization of the thy-1 gene. Furthermore, they have revealed the existence of a sequence which would encode an extra segment (31 amino acids) at the carboxyl terminus of the thy-1 molecule. These extra amino acids include a 20-amino acid hydrophobic segment which may be responsible for integration of thy-1 within the plasma membrane.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Seki, T -- Chang, H C -- Moriuchi, T -- Denome, R -- Ploegh, H -- Silver, J -- CA38404/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1985 Feb 8;227(4687):649-51.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2857501" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Animals ; Antigens, Surface/*genetics ; Antigens, Thy-1 ; Base Sequence ; Cloning, Molecular ; DNA, Recombinant/metabolism ; Membrane Proteins/*genetics/metabolism ; Mice ; Molecular Weight ; Nucleic Acid Hybridization ; Rats
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 80
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    A macrophage factor inhibits adipocyte gene expression: an in vitro model of cachexia (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-08-30
    Beschreibung: Certain infections and malignancies in mammals cause the development of a condition known as cachexia in which the animal continues to lose weight, often while consuming an adequate diet. When macrophages are stimulated with an endotoxin, they produce a factor or factors, termed cachectin, that inhibits the activity of fat-producing (lipogenic) enzymes in cultured adipocytes. This effect may reflect one of the physiological bases for cachexia. In the present study, clones of complementary DNA from genes whose expression is increased during the differentiation of adipocytes were used to study the molecular basis of cachectin's actions. In the presence of cachectin, the expression of the corresponding genes was reversibly and specifically inhibited. Furthermore, when mature adipocytes were exposed to cachectin, the messenger RNA's of those genes diminished and rapidly approached the levels present before differentiation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Torti, F M -- Dieckmann, B -- Beutler, B -- Cerami, A -- Ringold, G M -- AM0131401/AM/NIADDK NIH HHS/ -- GM25821/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Aug 30;229(4716):867-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3839597" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Actins/genetics ; Adipose Tissue/cytology/*enzymology ; Animals ; Cachexia/*etiology ; Cell Differentiation ; Cell Division/drug effects ; Cell Line ; Cell Survival/drug effects ; Dna ; Endotoxins/pharmacology ; Gene Expression Regulation/drug effects ; Glycerolphosphate Dehydrogenase/genetics ; Lipids/*biosynthesis ; Macrophages/*metabolism ; Models, Biological ; Proteins/*pharmacology ; RNA, Messenger/genetics ; Tumor Necrosis Factor-alpha
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 81
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    Cyclic AMP regulation of eukaryotic gene transcription by two discrete molecular mechanisms (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1985-07-19
    Beschreibung: In experiments designed to study the mechanism by which peptide hormones binding to their plasma membrane receptors stimulate the expression of specific genes, the transcription of two neuroendocrine genes, prolactin and growth hormone, was analyzed in a rat pituitary cell line. The results showed that cyclic adenosine monophosphate (cyclic AMP) stimulates the transcription of discrete subsets of eukaryotic genes by at least two independent molecular mechanisms. Cyclic AMP stimulated growth hormone gene transcription and phosphorylation of a 19,000-dalton nuclear protein; this appears to reflect direct nuclear actions of the cyclic AMP-dependent protein kinase. In contrast, the stimulation by cyclic AMP of prolactin gene transcription appears to reflect activation of a discrete calcium-dependent event.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Waterman, M -- Murdoch, G H -- Evans, R M -- Rosenfeld, M G -- New York, N.Y. -- Science. 1985 Jul 19;229(4710):267-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2990047" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cell Line ; Cell Membrane/metabolism ; Cobalt/pharmacology ; Colforsin ; Cyclic AMP/*physiology ; Diterpenes/pharmacology ; Growth Hormone/biosynthesis/genetics ; Phosphorylation ; Pituitary Gland/cytology ; Prolactin/biosynthesis/genetics ; Protein Kinases/physiology ; Rats ; Thyrotropin-Releasing Hormone/pharmacology ; *Transcription, Genetic/drug effects
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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