Publication Date:
1987-06-05
Description:
The v-sis oncogene encodes a platelet-derived growth factor (PDGF)-related product whose transforming activity is mediated by its functional interaction with the PDGF receptor. PDGF, as well as processed forms of the v-sis gene product, is a disulfide-linked dimer with eight conserved cysteine residues in the minimum region necessary for biologic activity. Site-directed mutagenesis of the v-sis gene revealed that each conserved cysteine residue was required directly or indirectly for disulfide-linked dimer formation. However, substitution of serine for cysteine codons at any of four positions had no detrimental effect on transforming activity of the encoded v-sis protein. These results establish that interchain disulfide bonds are not essential in order for this protein to act as a functional ligand for the PDGF receptor. The remaining four substitutions of serine for cysteine each inactivated transforming function of the molecule. In each case this was associated with loss of a conformation shown to involve intramolecular disulfide bonds. These studies provide insight into the role of individual cysteine residues in determining the structure of the sis/PDGF molecule critical for biological activity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Giese, N A -- Robbins, K C -- Aaronson, S A -- New York, N.Y. -- Science. 1987 Jun 5;236(4806):1315-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3035718" target="_blank"〉PubMed〈/a〉
Keywords:
Antigens, Polyomavirus Transforming
;
Antigens, Viral, Tumor/analysis/*physiology
;
Cell Transformation, Viral
;
Cross Reactions
;
Cysteine
;
*Genes, Viral
;
Mutation
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Oncogene Proteins, Viral/analysis/*physiology
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*Oncogenes
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Platelet-Derived Growth Factor/analysis/physiology
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Protein Conformation
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Protein Processing, Post-Translational
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Receptors, Cell Surface/metabolism
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Receptors, Platelet-Derived Growth Factor
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Retroviridae/*genetics
;
Sarcoma Virus, Woolly Monkey/*genetics
;
Serine
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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