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  • Nitrogen fixation  (65)
  • Triticum aestivum  (64)
  • Springer  (128)
  • Cell Press
  • Elsevier
  • Institute of Physics
  • 1985-1989  (128)
  • 1980-1984
  • 1987  (73)
  • 1985  (55)
Collection
Keywords
Publisher
  • Springer  (128)
  • Cell Press
  • Elsevier
  • Institute of Physics
Years
  • 1985-1989  (128)
  • 1980-1984
Year
  • 1
    Electronic Resource
    Electronic Resource
    Nitrogen-fixing stem nodules on Aeschynomene afraspera (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 61-66 
    Details
    ISSN: 1432-0789
    Keywords: Stem nodulation ; Aeschynomene afraspera ; Legume ; Nitrogen fixation ; Acetylene reduction assay (ARA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Aeschynomene afraspera is a wild annual legume growing in periodically waterlogged soils in western Africa. This legume is characterized by a profuse stem nodulation. Nodules are formed on the stem at the emergence of lateral root primordia, called nodulation sites. These sites are irregularly distributed on vertical rows all along the stem and branches. Stem nodules are hemispherically shaped. Their outside is dark green and they contain a red-pigmented central zone. Stem nodules exhibit a high nitrogen-fixing potential. Acetylene reduction assays result in stem nodule activity of 309 μmol C2H4 g−1 dry nodule h−1. Field-grown stem nodulated Aeschynomene accumulated more N (51 g N m−2 in 10 weeks) than the root nodulated one. Because of this nitrogenfixing potential and its ability to grow in waterlogged conditions, A. afraspera could probably be introduced into tropical rice cropping systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280352
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  • 2
    Electronic Resource
    Electronic Resource
    Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus (1987)
    Springer
    Biology and fertility of soils 3 (1987), S. 199-204 
    Details
    ISSN: 1432-0789
    Keywords: Phosphatases ; Rhizosphere ; Organic phosphorus ; Allium cepa ; Brassica oleracea ; Triticum aestivum ; Trifolium alexandrinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The distribution of phosphatase activity and of phosphate fractions of the soil in the proximity of roots was studied in order to evaluate the significance of phosphatases in P nutrition of various plants (Brassica oleracea, Allium cepa, Triticum aestivum, Trifolium alexandrinum). A considerable increase in both acid and alkaline phosphatase activity in all the four soil-root interfaces was observed. Maximum distances from the root surface at which activity increases were observed ranged from 2.0 mm to 3.1 mm for acid phosphatase and from 1.2 mm to 1.6 mm for alkaline phosphatase. The increase in phosphatase activity depended upon plant age, plant species and soil type. A significant correlation was noticed between the depletion of organic P and phosphatase activity in the rhizosphere soil of wheat (r = 0.99**) and clover (r = 0.97**). The maximum organic P depletion was 65% in clover and 86% in wheat, which was observed within a distance from the root of 0.8 mm in clover and 1.5 mm in wheat. Both the phosphatases in combination appear to be responsible for the depletion of organic P.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00640630
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  • 3
    Electronic Resource
    Electronic Resource
    Carbon transfer in a winter wheat (Triticum aestivum) ecosystem (1987)
    Springer
    Biology and fertility of soils 5 (1987), S. 76-82 
    Details
    ISSN: 1432-0789
    Keywords: Biomass accumulation ; Decomposition ; Litter ; Soil organic matter ; Soil respiration ; 14C deposition ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In a field experiment with 14C-labeled winter wheat conducted in the north-central region of the United States, crop-accumulated carbon (grain excluded) returned to the soil was found to be 542 g m−2 year−1. Almost half of the carbon from the underground compartment was released in the form of CO2 during the first 3 months after harvest due to very favorable conditions for biological activity. After 18 months, no less than 80% of the carbon from the plant residues was mineralized. About 16% of straw carbon and 24% of root carbon was transferred into soil organic matter. The annual rate of soil organic matter decomposition was approximated as 1.7%.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
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    Effect of inoculum rate on the performance of chickpea (Cicer arietinum L.) Rhizobium strains in the field (1987)
    Springer
    Biology and fertility of soils 5 (1987), S. 83-87 
    Details
    ISSN: 1432-0789
    Keywords: Inoculation ; Inoculum dose ; Nitrogen fixation ; Chickpea ; Rhizobium spp. ; Cicer arietinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of three inoculum rates on the performance of three chickpea (Cicer arietinum L.) Rhizobium strains was examined in the field on a Mollisol soil. Increasing amounts of inoculum improved the performance of the strains. A normal dose (104 cells per seed) applied at different intervals gave non-significant increases in nodulation, nitrogenase activity (acetylene reduction assay), nitrogen uptake and grain yield. A ten-fold increase in inoculum increased nodule number, shoot dry weight, nitrogenase activity (ARA) and grain yield, but increases over the control were significant only for nodule dry weight and nitrogen uptake by shoot and grain. The highest level of inoculum (100 × normal) significantly increased nodule dry weight, grain yield, total nitrogenase activity (ARA) and nitrogen uptake by shoot and grain. Strain TAL 620 was more effective than the other two. Combined nitrogen (60 kg N ha−1) suppressed nodulation and nitrogenase activity (ARA).
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  • 5
    Electronic Resource
    Electronic Resource
    Yield response of spring wheat cultivars (Triticum aestivum and T. turgidum) to inoculation with Azospirillum brasilense under field conditions (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 27-35 
    Details
    ISSN: 1432-0789
    Keywords: Triticum aestivum ; T. turgidum ; Nitrogen fixation ; Field inoculation ; Acetylene reduction assay (ARA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eight commercial Israeli spring wheat cultivars (six Triticum aestivum and two T. turgidum) grown with 40 and 120 kg N/ha were tested for responses to inoculation with Azospirillum brasilense. At the low level of N fertilization (40 kg/ha), five cultivars showed significant increases in plant dry weight measured at the milky ripe stage; however, by maturation only the cultivar “Miriam” showed a significant increase in grain yield. Two cultivars, which had shown a positive inoculation effect at the earlier stages, had a significant decrease in grain yield. No significant effect of inoculation was found at the high N level. To confirm those results, four wheat (T. aestivum) cultivars were tested separately over 4 years in 4 different locations under varying N levels. Only Miriam showed a consistently positive effect of Azospirillum inoculation on grain yield. Inoculation increased the number of roots per plant on Miriam compared with uninoculated plants. This effect was found at all N levels. Nutrient (N, P and K) accumulation and number of fertile tillers per unit area were also enhanced by Azospirillum, but these parameters were greatly affected by the level of applied N. It is suggested that the positive response of the spring wheat cultivar “Miriam” to Azospirillum inoculation is due to its capacity to escape water stresses at the end of the growth season.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280347
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  • 6
    Electronic Resource
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    Nitrogen fixation and balance studies of rice soil (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 15-19 
    Details
    ISSN: 1432-0789
    Keywords: Nitrogen fixation ; N-balance studies ; Azolla ; Blue-green algae ; Chemical N fertilization ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A nitrogen balance study conducted in ceramic pots under net house conditions for four seasons showed that flooded rice soil leaves a positive nitrogen balance (N increase) in soil after rice cropping in both fertilized and unfertilized soil. Recovery of nitrogen from rice soil was more than its input in unfertilized soil, but it was reverse in fertilized soil. Incorporation of Azolla or BGA twice as basal and 20 days after transplanting (DAT) alone or in combination showed higher nitrogen balance and N2-fixation (N gain) in soil than in that where it was applied once either as basal or 20 DAT. Planted soil showed more N2-fixation than that of fallow rice, and flooded soil fixed more nitrogen in comparison to non-flooded soil in light but less in dark. Soil exposed to light fixed more nitrogen than that of unexposed soil in both flooded and non-flooded conditions.
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    URL: http://dx.doi.org/10.1007/BF00280345
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  • 7
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    Stimulation of root exudation of rice seedlings by Azospirillum strains: carbon budget under gnotobiotic conditions (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 9-14 
    Details
    ISSN: 1432-0789
    Keywords: Rhizosphere ; Nitrogen fixation ; Root exudates ; Soil bacteria ; Carbon budget ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The association of rice seedlings (cv. Delta) with different strains of Azospirillum was studied under monoxenic conditions in the dark. Axenic 3-day-old seedlings were obtained on a C- and N-free medium and inoculated with 6 · 107 bacteria per plant in a closed vial. Seven days later, different components of a carbon budget were evaluated on them and on sterile controls: respired CO2, carbon of shoot and roots, bacterial and soluble carbon in the medium. Two strains (A. lipoferum 4B and A. brasilense A95) isolated from the rhizosphere of rice caused an increase in exudation, + 36% and + 17% respectively compared with sterile control. Shoot carbon incorporation and respiration were reduced by inoculation. A third strain (A. brasilense R07) caused no significant change in exudation. A. lipoferum B7C isolated from maize did not stimulate rice exudation either. We further investigated a possible effect of nitrogen fixation on this phenomenon: inhibition of nitrogen fixation by 10% C2H2 did not modify the extent of C exudation by rice associated with A. lipoferum 4B or with the non-motile A. lipoferum 4T.
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    URL: http://dx.doi.org/10.1007/BF00280344
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  • 8
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    Peculiar effect of Azospirillum inoculation on growth and nitrogen balance of winter wheat (Triticum aestivum) (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 55-59 
    Details
    ISSN: 1432-0789
    Keywords: Azospirillum brasilense ; Triticum aestivum ; Inoculation ; N and dry matter yield ; N percentages in plant parts ; Associative N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat plants (Triticum aestivum) grown in pots and in the field under the Mediterranean climate of the south of France were inoculated with a strain of Azospirillum brasilense. Comparisons with non-inoculated plants grown under the same conditions showed significant responses to inoculation with an increase in the number of fertile tillers, shoot and root dry weight, and root to shoot biomass ratio. The roots of inoculated plants attracted relatively more assimilates than those of the control plants until a late stage of growth (heading stage) but the rhizosphere respiration expressed per unit of root growth was not increased by inoculation. Nitrogen yield, both total and in grains, was also enhanced; however, N percentages of all aerial parts of the plants grown in pots were always statistically lower after inoculation than in the control. At maturity, the N % in seeds was 1.81 and 2.45, respectively. The possible mechanisms of this effect of inoculation under the experimental conditions of this study are discussed.
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  • 9
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    Inoculation of field-grown wheat (Triticum aestivum) with Azospirillum spp. in Brazil (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 37-40 
    Details
    ISSN: 1432-0789
    Keywords: Azospirillum brasilense ; Azospirillum amazonense ; rate reductase ; Inoculation ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three field experiments with wheat were conducted in 1983, 1984, and 1985 in Terra Roxa soil in Paraná, the major Brazilian wheat-growing region, to study inoculation effects of various strains of Azospirillum brasilense and A. amazonense. In all three experiments inoculation with A. brasilense Sp 245 isolated from surface-sterilized wheat roots in Paraná produced the highest plant dry weights and highest N% in plant tops and grain. Grain yield increases with this strain were up to 31 % but were not significant. The application of 60 or 100 kg N ha−1 to the controls increased N accumulation and produced yields less than inoculation with this strain. Another A. brasilense strain from surface-sterilized wheat roots (Sp 107st) also produced increased N assimilation at the lower N fertilizer level but reduced dry weights at the high N level, while strain Sp 7 + Cd reduced dry weights and N% in the straw at both N levels. The A. amazonense strain isolated from washed roots and a nitrate reductase negative mutant of strain Sp 245 were ineffective. Strains Sp 245 and Sp 107st showed the best establishment within roots while strain Cd established only in the soil.
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  • 10
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    Colonization of wheat seedling (Triticum aestivum) roots by Pseudomonas fluorescens and Bacillus subtilis (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 41-46 
    Details
    ISSN: 1432-0789
    Keywords: Competition ; Migration ; Colonization potential ; Replica printing ; Triticum aestivum ; Pseudomonas fluorescens ; Bacillus subtilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Colonization patterns of Pseudomonas fluorescens and Bacillus subtilis on roots of wheat seedlings growing on water agar were studied qualitatively by replica printing and quantitatively by the plate count method. The results indicated a stronger colonization potential for P. fluorescens (up to 107 cfu/cm root) than for B. subtilis (up to 105 cfu/cm root). Although the numbers of both species were lower when inoculated together, the observed colonization patterns on the roots were comparable to those found with single inoculations. For none of these bacteria was active migration along the root surface in any direction observed, indicating that distal positions are reached mainly by a passive displacement on the root tip and elongating cells. Ecological implications of the observed phenomena are discussed.
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  • 11
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    Effect of certain herbicides on growth and pathogenicity of take-all fungus on wheat (Triticum aestivum L.) (1987)
    Springer
    Biology and fertility of soils 5 (1987), S. 31-35 
    Details
    ISSN: 1432-0789
    Keywords: Gaeumannomyces graminis ; VegVetative growth ; Pathogenicity ; Herbicides ; Diquat+paraquat ; Glyphosate ; Dicamba ; Trifluralin ; Chlorsulfuron ; Chlorthal dimethyl ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of Spray Seed (diquat + paraquat), Roundup (glyphosate), Banvel-D (dicamba), Treflan (trifluralin), Glean (chlorsulfuron) and Dacthal (chlorthal dimethyl) at concentrations of 0–500 ppm product on the vegetative growth, vigour and pathogenicity of Gaeumannomyces graminis var. tritici (Ggt) on wheat were examined. All herbicides with the exception of dicamba and chlorsulfuron inhibited fungal growth on potato dextrose agar (PDA) at concentrations 10–500-fold of rates recommended for use in the field. The vegetative growth of the pathogen growing out of straw colonized on PDA supplemented with 100 ppm diquat + paraquat or glyphosate was reduced by 47.4% and 42.4%, respectively. When portions of these colonies were subcultured onto unamended PDA, their growth and the pathogenicity of straw pieces colonized by these subcultures were found to be unaltered. Straw colonized by Ggt on agar amended with concentrations of diquat + paraquat or at all concentrations of glyphosate produced less root disease in wheat seedlings in comparison to those colonized on unamended agar. It is proposed that the reduced pathogenicity of inocula prepared on agar amended with these two herbicides is due to poor colonization by the pathogen of straw on these media, and that a similar effect on saprophytic colonization in the field could lead to a reduction in the field inocula of the pathogen.
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    URL: http://dx.doi.org/10.1007/BF00264343
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  • 12
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    The effects of nitrogen fixation, soil nitrate, and defoliation on the growth, alkaloids, and nitrogen levels of Lupinus succulentus (Fabaceae) (1987)
    Springer
    Oecologia 74 (1987), S. 425-431 
    Details
    ISSN: 1432-1939
    Keywords: Lupinus succulentus ; Fabaceae ; Lupine ; Quinolizidine alkaloids ; Nitrogen fixation ; Defoliation ; Plant-herbivore interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We examined the effects of nitrogen nutrition and defoliation on the alkaloids, nitrogen levels, and growth of Lupinus succulentus by growing plants under five nitrogen/defoliation treatments: 1) fertilization with a high-nitrate nutrient solution, 2) fertilization with a low-nitrate solution, 3) inoculation with N-fixing bacteria but without available soil nitrogen, 4) high-nitrate solution plus periodic partial defoliation, and 5) low-nitrate plus defoliation. In the absence of defoliation, plants from both the N-fixing and high-N treatments had higher concentrations of alkaloids and nitrogen, and higher growth rates than the low-N plants. Periodic defoliation had little effect on the high-N plants, but defoliated N-fixing plants were severely stunted and had lower alkaloid and nitrogen levels. The experimental treatments also affected the relative concentrations of the alkaloids. Our results indicate that 1) alkaloid composition and concentration in L. succulentus are determined by both nitrogen availability and developmental state, 2) plants relying solely on N-fixation respond quite differently to defoliation than those with adequate soil nitrogen, and 3) the food value of the plant tissue can be affected by an interaction between the effects of defoliation and nitrogen status.
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    URL: http://dx.doi.org/10.1007/BF00378940
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  • 13
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    Extensive mitochondrial DNA variation in somatic tissue cultures initiated from wheat immature embryos (1987)
    Springer
    Current genetics 12 (1987), S. 369-376 
    Details
    ISSN: 1432-0983
    Keywords: Triticum aestivum ; Tissue culture ; Mitochondrial DNA ; Genomic variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Wheat mitochondria) DNA has been isolated from callus cultures initiated from both immature embryos and the corresponding parental cultivar. A Sall restriction pattern study has shown that the organization of callus culture mitochondria) DNA underwent extensive change, characterized by either the disappearance or the decrease in the relative stoichiometry of several restriction bands. Hybridization of labelled mitochondrial fragments obtained from a recombinant cosmid library to Southern blots of callus and parental line restricted mitochondria) DNAs has shown that a fraction of the mitochondria) genome was lost in callus cultures. Data from a Sall + HindIII restriction map of a defined part of the wheat mitochondria) genome concerned with some of these variations strongly suggest that the observed variations correspond to the disappearance of at least one mitochondria) DNA subgenomic molecule in callus cultures.
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    URL: http://dx.doi.org/10.1007/BF00405759
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  • 14
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    New types of acetate-oxidizing, sulfate-reducing Desulfobacter species, D. hydrogenophilus sp. nov., D. latus sp. nov., and D. curvatus sp. nov. (1987)
    Springer
    Archives of microbiology 148 (1987), S. 286-291 
    Details
    ISSN: 1432-072X
    Keywords: Sulfate-reducing bacteria ; Desulfobacter species ; Acetate ; Hydrogen ; Autotrophic growth ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sulfate-reducing bacteria with oval to rod-shaped cells (strains AcRS1, AcRS2) and vibrio-shaped cells (strains AcRM3, AcRM4, AcRM5) differing by size were isolated from anaerobic marine sediment with acetate as the only electron donor. A vibrio-shaped type (strain AcKo) was also isolated from freshwater sediment. Two strains (AcRS1, AcRM3) used ethanol and pyruvate in addition to acetate, and one strain (AcRS1) grew autotrophically with H2, sulfate and CO2. Higher fatty acids or lactate were never utilized. All isolates were able to grow in ammonia-free medium in the presence of N2. Nitrogenase activity under such conditions was demonstrated by the acetylene reduction test. The facultatively lithoautotrophic strain (AcRS1), a strain (AcRS2) with unusually large cells (2×5 μm), and a vibrio-shaped strain (AcRM3) are described as new Desulfobacter species, D. hydrogenophilus, D. latus, and D. curvatus, respectively.
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    URL: http://dx.doi.org/10.1007/BF00456706
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  • 15
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    The calcium requirement for functional vesicle development and nitrogen fixation by Frankia strains EAN1pec and CpI1 (1987)
    Springer
    Archives of microbiology 149 (1987), S. 24-29 
    Details
    ISSN: 1432-072X
    Keywords: Frankia ; Nitrogen fixation ; Calcium ; Vesicle development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A calcium requirement was shown for both vesicle development and nitrogenase activity by Frankia strains EAN1pec and CpI1. Washing cells with EGTA or EDTA inhibited both vesicle development and nitrogenase activity. The inhibition of both was reversed by the addition of calcium. A variety of agents known to affect calcium-dependent biological processes, such as a Ca-ATPase inhibitor, Ca-channel blockers, Ca-ionophores, calmodulin antagonists and the local anaesthetics, tetracaine and dibucaine, inhibited nitrogenase activity. Respiratory studies showed that a CN-insensitive respiration process occurred only under nitrogen derepressing conditions. Respiration by NH4Cl-grown cells was completely inhibited by KCN while N2-grown cells were inhibited by only 70%. Removal of calcium ions by EGTA or by the addition of dibucaine or tetracaine blocked the CN-insensitive respiration. This CN-insensitive respiration may be involved in protecting nitrogenase inside the vesicles from oxygen.
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    URL: http://dx.doi.org/10.1007/BF00423131
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  • 16
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    Comparative physiology of nitrogenase activity and vesicle development for Frankia strains CpI1, ACN1 ag , EAN1pec and EUN1f (1987)
    Springer
    Archives of microbiology 147 (1987), S. 383-388 
    Details
    ISSN: 1432-072X
    Keywords: Frankia ; Nitrogen fixation ; Vesicle development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between nitrogen fixation and development of a specialized cell structure, called the vesicle, was studied using four Frankia isolates. Nitrogenase activity was repressed in all four strains during growth with ammonia. Strain CpI1 formed no vesicles during NH4 growth. Strains ACN1 ag , EAN1pec and EUN1f produced low numbers of vesicles in the presence of ammonia. Following transfer to nitrogen-free media, a parallel increase in nitrogenase activity and vesicle numbers occurred with all four isolates. Appearance of nitrogenase activity was more rapid in those strains that possessed some vesicles at the time of shift to N2 as a nitrogen source. The ratio of vesicle numbers to level of nitrogenase activity varied widely among the four strains and in response to different growth conditions and culture age of the individual strains. Optimum conditions of temperature, carbon and energy source, nitrogen source and availability of iron and molybdenum were different for each of the four strains. Those conditions that significantly reduced nitrogenase activity were always associated with decreased numbers of vesicles.
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    URL: http://dx.doi.org/10.1007/BF00406137
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  • 17
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    Localization of nitrogenase in vesicles of Frankia sp. Cc1.17 by immunogoldlabelling on ultrathin cryosections (1987)
    Springer
    Archives of microbiology 146 (1987), S. 327-331 
    Details
    ISSN: 1432-072X
    Keywords: Actinomycetes ; Nitrogen fixation ; Symbiosis ; Immunocytochemistry ; Ultracryotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunogoldlabelling on ultrathin cryosections of Frankia sp. Cc1.17 showed specific labelling of nitrogenase in the spherical cells called vesicles. No label was found in the hyphae in any cells grown on a medium with combined nitrogen, nor in those to which no specific antiserum was added. Similar results were obtained with cultures grown under high (20%) and low (2%) oxygen tension in the gas phase.
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    URL: http://dx.doi.org/10.1007/BF00410930
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  • 18
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    The relationship between hydrogen metabolism, sulfate reduction and nitrogen fixation in sulfate reducers (1987)
    Springer
    Journal of industrial microbiology and biotechnology 1 (1987), S. 383-388 
    Details
    ISSN: 1476-5535
    Keywords: Sulfate-reducing bacteria ; Hydrogen metabolism ; Nitrogen fixation ; Deuterium-proton exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Hydrogenase and nitrogenase activities of sulfate-reducing bacteria allow their adaptation to different nutritional habits even under adverse conditions. These exceptional capabilities of adaptation are important factors in the understanding of their predominant role in problems related to anaerobic metal corrosion. Although the D2−H+ exchange reaction indicated thatDesulfovibrio desulfuricans strain Berre-Sol andDesulfovibrio gigas hydrogenases were reversible, the predominant activity in vivo was hydrogen uptake. Hydrogen production was restricted to some particular conditions such as sulfate or nitrogen starvation. Under diazotrophic conditions, a transient hydrogen evolution was followed by uptake when dinitrogen was effectively fixed. In contrast, hydrogen evolution proceeded when acetylene was substituted as the nitrogenase substrate. Hydrogen can thus serve as an electron donor in sulfate reduction and nitrogen metabolism.
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    URL: http://dx.doi.org/10.1007/BF01569336
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  • 19
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    Genetic control of NADH dehydrogenase-1 and aromatic alcohol dehydrogenase-2 in hexaploid wheat (1987)
    Springer
    Biochemical genetics 25 (1987), S. 837-846 
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    ISSN: 1573-4927
    Keywords: NADH dehydrogenase ; aromatic alcohol dehydrogenase ; Triticum aestivum ; isozymes ; structural genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The genetic control of NADH dehydrogenase-1 (NDH-1) and aromatic alcohol dehydrogenase-2 (AADH-2) was investigated in Triticum aestivum cv. Chinese Spring. Evidence was obtained that NDH-1 is active as a monomer and is encoded by genes located in the p arms of the homoeologous group 4 chromosomes. The NDH-1 gene loci located in 4 Ap, 4Bp, and 4Dp were designated Ndh-A1, Ndh-B1, and Ndh-D1, respectively. Aadh-A2 was previously reported to be located in 6Aq; in this study, Aadh-B2 and Aadh-D2 were localized in 6Bq and 6Dq, respectively. Alcohol dehydrogenase-1 is expressed on AADH-2 zymograms; the presence of a contaminating aliphatic alcohol in one or more reagents is suggested as the probable cause of this phenomenon.
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    URL: http://dx.doi.org/10.1007/BF00502603
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    The pattern of amyloplast DNA accumulation during wheat endosperm development (1987)
    Springer
    Planta 171 (1987), S. 416-421 
    Details
    ISSN: 1432-2048
    Keywords: Amyloplast DNA ; DNA accumulation ; Endosperm development ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The accumulation of amyloplast DNA during endosperm development was studied in two cultivars of spring wheat, Triticum aestivum L. ‘Chinese Spring’ (CS) and ‘Spica’, small and relatively larger-grained cultivars, respectively. Endosperms were isolated between 9 and 45 days post anthesis (dpa) and the amyloplast DNA content of endosperm nucleic-acid extracts was measured by quantitative hybridisation with a homologous chloroplast-DNA probe. The endosperm cells of CS and Spica accumulated amyloplast DNA during development in a similar way. In both cultivars there was a large increase in the amount of plastid DNA (ptDNA) per endosperm between 9 and about 15 dpa, after which there was no further increase. Because nuclear DNA continued to accumulate until 24 dpa, the percentage contribution of amyloplast DNA to total DNA fluctuated in both cultivars during development, reaching maxima at 12 dpa of about 1.00% and 0.85%, and dropping to apparently constant levels of 0.60% and 0.52% in CS and Spica, respectively, by 24 dpa. In both cultivars, the average number of ptDNA copies per amyloplast was calculated to increase from about 10 copies at 9 dpa to about 50 copies in the mature amyloplasts at 31 dpa. However, the heavier endosperms of Spica contain more cells than those of CS and the varieties therefore differed in the amount of ptDNA that accumulated per endosperm: Spica endosperms accumulated 110 ng of ptDNA by 15 dpa, compared with only 85 ng in CS. The apparent accumulation of ptDNA copies in wheat amyloplasts during endosperm development contrasts with the decline in chloroplast-DNA copies in wheat chloroplasts during leaf development.
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    URL: http://dx.doi.org/10.1007/BF00398688
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    Cellular compartmentation of ureide biogenesis in root nodules of cowpea (Vigna unguiculata (L.) Walp.) (1987)
    Springer
    Planta 172 (1987), S. 162-175 
    Details
    ISSN: 1432-2048
    Keywords: Nitrogen fixation ; Peroxisome ; Root nodules ; Ureide biogenesis ; Uricase ; Vigna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cowpea (Vigna unguiculata (L.) Walp.) nodules have been investigated by means of cytochemical and immunocytochemical procedures at the ultrastructural level in order to assess the role of the uninfected cells in ureide biogenesis. Uricase activity in the nodules was shown by cytochemical methods to be localized exclusively in the numberous large peroxisomes confined to the uninfected cells; the small peroxisomes in the infected cells did not stain for uricase. Uricase was also localized in the peroxisomes of uninfected cells by immunogold techniques employing polyclonal antibodies against nodule-specific uricase of soybean. There was no labeling above background of any structures in the infected cells. The results indicate that the uninfected cells are essential for ureide biogenesis in cowpea. Although tubular endoplasmic reticulum, the presumptive site of allantoinase, increases greatly in the uninfected cells during nodule development, it virtually disappears as the nodules mature. The inconsistency between the disappearance of the tubular endoplasmic reticulum from older nodules and the high allantoinase activity reported for older plants remains to be explained.
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    URL: http://dx.doi.org/10.1007/BF00394584
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    A somaclonal variant of wheat with additional β-amylase isozymes (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 459-464 
    Details
    ISSN: 1432-2242
    Keywords: β-amylase ; Isozymes ; Triticum aestivum ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The progeny of 149 plants regenerated from tissue culture of immature wheat (Triticum aestivum) embryos were screened for variation in their grain β-amylase isozyme pattern. One regenerant was found which was heterozygous for a variant pattern characterized by the presence of at least five new isozyme bands, as well as an increased intensity in existing bands in two more positions. The F2 of a homozygous variant crossed back to the parent segregated in an approximate 3∶1 ratio but resolution of the gels was not sufficient to distinguish whether this represents a dominant or co-dominant single mutant gene. No chromosome abnormalities were evident in mitosis or meiosis of the homozygous variant or in the F1 of the variant crossed back to the parent. No recombination has been seen between the variant bands and production of multiple bands from a single locus is consistent with the nature of the known β-amylase loci. However, the variant bands were not evident in a survey of 111 diverse genotypes, nor were they present in developing grain of the parent cultivar. Therefore, this variant could represent a rare mutation leading to expression of a currently unexpressed locus.
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    URL: http://dx.doi.org/10.1007/BF00262516
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    Genetics of resistance to Puccinia graminis tritici in ‘Chris’ and ‘W3746’ wheats (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 846-855 
    Details
    ISSN: 1432-2242
    Keywords: Puccinia graminis tritici ; Triticum aestivum ; Monosomic analysis ; Rust resistance ; Gene identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary ‘Chris’ wheat possessed genes Sr5, Sr7a, Sr8a, Sr9g and Sr12. ‘W3746’, derived from the cross ‘Chris’/‘Baart’, possessed Sr7a and Sr12. The response conferred by Sr7a was influenced by the genetic background. Although Sr7a or Sr12 alone conferred no observable resistance upon adult plants, the adult resistances of ‘Chris’ and ‘W3746’ to predominant pathotypes appeared to be associated with the interaction of Sr7a and Sr12, or genes at closely linked loci.
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    URL: http://dx.doi.org/10.1007/BF00289389
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    Chromosomal location of a K/Na discrimination character in the D genome of wheat (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 584-588 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; T. turgidum ; Aegilops squarrosa ; Cation uptake ; D genome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary K/Na ratios have been determined in the leaves of salt-treated plants of 14 disomic substitution lines in which each of the D-genome chromosomes replaces the homoeologous A- or B-genome chromosome in the tetraploid wheat variety Langdon (AABB genome). Aneuploid lines of hexaploid bread wheat (cv Chinese Spring) having a reduced or an enhanced complement of chromosome 4D have also been examined. These investigations show that the gene(s) determining K/Na ratios in the leaves of wheat plants grown in the presence of salt is located on the long arm of chromosome 4D.
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    URL: http://dx.doi.org/10.1007/BF00288856
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    Cytogenetic analysis of structural rearrangements in three varieties of common wheat, Triticum aestivum (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 635-645 
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    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Structural rearrangements ; Translocation ; Interchange ; Duplication-deficiency ; Aneuploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The winter wheat varieties ‘Starke’ and ‘Cappelle Desprez’ and the spring wheat ‘Chinese Spring’ were analysed for structural chromosome rearrangements that resulted in the formation of multivalents in F1 hybrids. The analyses were carried out using hybrids involving euploids, monosomic and ditelosomic stocks, and double-monotelodisomic constructs. The study confirmed that ‘Cappelle Desprez’ differs from ‘Chinese Spring’ in a reciprocal translocation between chromosomes 5B and 7B (Riley et al. 1967); a translocation involving chromosomes 3B and 3D could not be verified. Furthermore, the analysis showed that ‘Starke’ differs from ‘Chinese Spring’ in a reciprocal translocation between chromosomes 7A and 7D. Both translocations have a coefficient of multivalent realisation of about 0.84. Further multivalents in euploid ‘Starke’, in euploid and some aneuploid stocks of ‘Cappelle Desprez’, and in euploid as well as various types of aneuploid hybrids between all three varieties could nearly all be explained hypothesizing that chromosome 2B of both ‘Starke’ and ‘Cappelle Desprez’ is a duplication-deficiency chromosome. In the hypothesis a part of the long arm of 2B is missing and replaced by a duplicated part of the long arm of chromosome 2D. The multivalents of this rearrangement showed an average coefficient of realisation of about 0.09.
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    URL: http://dx.doi.org/10.1007/BF00260770
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    The peroxidase isozymes of the wheat kernel: tissue and substrate specificity and their chromosomal location (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 701-706 
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    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Peroxidases ; Tissue and substrate-specificity ; Chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Peroxidase isozymes were studied in the Triticum aestivum L. kernel and in nullisomic-tetrasomic and ditelocentric combinations of ‘Chinese Spring’ wheat. Analyses were carried out on different parts of dry kernels (embryo plus scutellum and endosperm) using polyacrylamide and starch gel electrophoresis, different electrophoretic buffer systems and various staining methods. The peroxidase isozymes showed a low substrate-specificity and a high tissue-specificity. The embryo plus scutellum and the endosperm always presented different peroxidase patterns. Endosperm peroxidases were associated with chromosome arms 7DS, 4BL and 7AS; whereas the embryo plus scutellum isozymes were related to chromosome arms 3AL, 3BL and 3DS. The different results obtained using various electrophoretic techniques are due to the buffer system used. All staining procedures employed revealed the same peroxidase isozymes.
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    URL: http://dx.doi.org/10.1007/BF00260779
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    Variability and genetics of spacer DNA sequences between the ribosomal-RNA genes of hexaploid wheat (Triticum aestivum) (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 617-624 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Ribosomal RNA ; Nor alleles ; Nucleolar organiser regions ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using restriction enzyme digests of genomic DNA extracted from the leaves of 25 hexaploid wheat (Triticum aestivum L. em. Thell.) cultivars and their hybrids, restriction fragment length polymorphisms of the spacer DNA which separates the ribosomal-RNA genes have been examined. (From one to three thousand of these genes are borne on chromosomes 1B and 6B of hexaploid wheat). The data show that there are three distinct alleles of the 1B locus, designated Nor-B1a, Nor-B1b, and Nor-B1c, and at least five allelic variants of the 6B locus, designated Nor-B2a, Nor-B2b, Nor-B2c, Nor-B2d, and Nor-B2e. A further, previously reported allele on 6B has been named Nor-B2f. Chromosome 5D has only one allelic variant, Nor-D3. Whereas the major spacer variants of the 1B alleles apparently differ by the loss or gain of one or two of the 133 bp sub-repeat units within the spacer DNA, the 6B allelic variants show major differences in their compositions and lengths. This may be related to the greater number of rDNA repeat units at this locus. The practical implications of these differences and their application to wheat breeding are discussed.
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    URL: http://dx.doi.org/10.1007/BF00288861
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    A new selection criterion for yield in wheat (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 275-279 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Biomass ; Harvest index ; Selection criterion ; Tiller mortality ; Modified selection procedure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mortality of young tillers to an extent of 36.5% under optimum cultural conditions was recorded in a field experiment. Attention is drawn to the necessity of minimising this loss and to diverting it towards productive tillers by applying selection pressure. Future yield advances may be achieved by selecting genotypes which tiller moderately in the vegetative phase, most of which survive to produce grains. The character association of each variety under consideration was studied for the suitability towards this objective. A modified selection procedure is suggested which may be advantageously applied to achieve this objective. The proposed new methodology may also be effectively applied to such other cereal crops as barley, triticale and oats.
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    URL: http://dx.doi.org/10.1007/BF00289980
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    Cloning of the gene for phosphoenolpyruvate carboxylase from Azotobacter chroococcum, an enzyme important in aerobic nitrogen fixation (1987)
    Springer
    Molecular genetics and genomics 208 (1987), S. 481-484 
    Details
    ISSN: 1617-4623
    Keywords: PEP carboxylase ; Azotobacter chroococcum ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Azotobacter chroococcum Fos 189 is a Tn1-induced mutant which, unlike the parent strain MCD1, does not fix nitrogen in air when provided with glucose or pyruvate as sole carbon sources. Fos 189 showed 5% of parental activity for phosphoenolpyruvate carboxylase though PEP synthetase activity was normal. The A. chroococcum phosphoenolpyruvate carboxylase (ppc) gene was isolated after complementation of an appropriate Escherichia coli mutant using a broad host range gene bank prepared from A. chroococcum genomic DNA. The gene was localised by transposon mutagenesis and subcloning on a minimum DNA fragment of 6.6 kb. Broad host range plasmids containing the A. chroococcum ppc gene complemented the mutation in Fos 189 thereby restoring aerotolerant nitrogen fixation.
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    URL: http://dx.doi.org/10.1007/BF00328143
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    Identification of nifE-, nifN- and nifS-like genes in Bradyrhizobium japonicum (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 503-508 
    Details
    ISSN: 1617-4623
    Keywords: Bradyrhizobium ; Nif genes ; Nitrogen fixation ; Root nodule symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 17 kb region between the Bradyrhizobium japonicum nitrogenase genes (nifDK and nifH) was investigated for the presence of further nif or fix genes by site-directed insertion or deletion/replacement mutagenesis and interspecies hybridization. Mutant strains were tested for their ability to reduce acetylene in free-living, microaerobic culture (Nif phenotype) and in soybean root nodules (Fix phenotype). The presence of a gene, previously identified by hybridization with the Klebsiella pneumoniae nifB gene, was proved by isolation of a nifB insertion mutant which was completely Nif- and Fix-. Three other regions were found to be homologous to the K. pneumoniae genes nifE, nifN, and nifS, NifE and nifN insertion mutants were completely Nif-/Fix- whereas nifS mutants were leaky with 30% residual Fix activity. Taken together, the data show that the B. japonicum genome harbours a cluster of closely adjacent genes which are directly concerned with nitrogenase function.
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    URL: http://dx.doi.org/10.1007/BF00331622
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    Cloning and characterization of nifA and ntrC genes of the stem nodulating bacterium ORS571, the nitrogen fixing symbiont of Sesbania rostrata: Regulation of nitrogen fixation (nif) genes in the free living versus symbiotic state (1987)
    Springer
    Molecular genetics and genomics 206 (1987), S. 207-219 
    Details
    ISSN: 1617-4623
    Keywords: Azorhizobium sesbaniae ORS571 ; Nitrogen fixation ; Regulation ; Tn5 mutagenesis ; lacZ fusions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cosmid bank of ORS571, a diazotrophic bacterium capable of inducing aerial stem and root nodules on Sesbania rostrata, was constructed in the vector pLAFR1. A DNA probe carrying the Klebsiella pneumoniae nifA gene was used to identify nifA-and ntrC-like regions of ORS571 in the cosmid bank by colony hybridization. Cosmids carrying these regions were mapped by restriction endonuclease analysis, Southern blotting and transposon Tn5 mutagenesis. Selected Tn5 insertion mutations in the nifA/ntrC homologous regions were used for gene-replacement experiments and the resulting ORS571 mutants were examined for Nif, Fix and Ntr phenotypes. Two clearly distinct regulatory loci were thus identified and named nifA and ntrC. Plasmids carrying gene fusions of the ORS571 nifH and nifD genes to lacZ were constructed and the regulation of the ORS571 nifHDK promoter, and of the Rhizobium meliloti nifHDK promoter, was studied under varying physiological conditions in ORS571, ORS571 nifA::Tn5 and ORS571 nitrC::Tn5 strains. A model for the role of nifA and ntrC in the regulation of ORS571 nif and other nitrogen assimilation genes is proposed.
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    Identification of two classes of Rhizobium phaseoli genes required for melanin synthesis, one of which is required for nitrogen fixation and activates the transcription of the other (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 155-160 
    Details
    ISSN: 1617-4623
    Keywords: Gene regulation ; Melanin synthesis ; Nitrogen fixation ; Phaseolus beans ; Rhizobium phaseoli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The symbiotic plasmid pRP2JI of Rhizobium phaseoli strain 8002 was shown to contain two separate regions of DNA which are required and sufficient for the synthesis of the pigment melanin. One of these regions containing the class II mel gene(s) was located to other genes involved in nodulation and in nitrogen fixation. Mutations in this region abolished both the ability to synthesize melanin and to fix nitrogen in Phaseolus bean root nodules. Mutations in the other, unlinked region, containing class I mel gene(s), also abolished melanin synthesis but did not affect symbiotic nitrogen fixation. Transcriptional fusions between the class I mel gene and the Escherichia coli lacZ gene were constructed and it was demonstrated that the class II mel gene(s) activated their transcription in free-living culture. Further, strains containing the cloned regulatory class II gene(s) synthesized melanin when growing in minimal medium, in contrast to wild-type strains which became pigmented only in complete medium containing yeast extract and tryptone. It was shown by hybridization experiments that the regulatory mel gene was closely linked to or may correspond to the regulatory nifA gene; a fragment of R. phaseoli DNA which included the class II gene(s) of R. phaseoli hybridized to a previously identified nifA-like gene of R. leguminosarum, the species that nodulates peas.
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    Transformation and regeneration of the legume Lotus corniculatus: A system for molecular studies of symbiotic nitrogen fixation (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 245-250 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium rhizogenes ; Plant transformation ; Transgenic legumes ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A procedure for transformation and regeneration of the legume species Lotus corniculatus (Bird's-foot trefoil) has been developed. The Agrobacterium rhizogenes 15834 and 8196 strains were used to transform plant cells in wound site infections and transformed roots were propagated in vitro. Transformation was monitored by hybridization with pRi T-DNA sequences and by detection of agropine and mannopine. Transformation frequencies of up to 90% were obtained. Shoots spontaneously formed on hairy root cultures were excised, rooted and inoculated with Rhizobium. Root nodules formed on transformed plants had nitrogenase activities comparable to untransformed nodules. Transcript levels from the nodule-specific leghemoglobin genes and the constitutive ubiquitin genes were similar in transformed and untransformed root nodules. Transformed plants responded to R. loti and Bradyrhizobium sp. (Lotus) strains with phenotypes identical to phenotypes for untransformed plants.
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    Identification and cloning of nodulation genes from the stem-nodulating bacterium ORS571 (1987)
    Springer
    Molecular genetics and genomics 206 (1987), S. 291-299 
    Details
    ISSN: 1617-4623
    Keywords: Nitrogen fixation ; Stem nodulation ; Tn5 mutagenesis ; nod genes ; nodC homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary After random Tn5 mutagenesis of the stem-nodulating Sesbania rostrata symbiont strain ORS571, Nif-, Fix- and Nod- mutants were isolated. The Nif- mutants had lost both free-living and symbiotic N2 fixation capacity. The Fix- mutants normally fixed N2 in the free-living state but induced ineffective nodules on S. rostrata. They were defective in functions exclusively required for symbiotic N2 fixation. A further analysis of the Nod- mutants allowed the identification of two nod loci. A Tn5 insertion in nod locus 1 completely abolished both root and stem nodulation capacity. Root hair curling, which is an initial event in S. rostrata root nodulation, was no longer observed. A 400 bp region showing weak homology to the nodC gene of Rhizobium meliloti was located 1.5 kb away from this nod Tn5 insertion. A Tn5 insertion in nod locus 2 caused the loss of stem and root nodulation capacity but root hair curling still occurred. The physical maps of a 20.5 kb DNA region of nod locus 1 and of a 40 kb DNA region of nod locus 2 showed no overlaps. The two nod loci are not closely linked to nif locus 1, containing the structural genes for the nitrogenase complex (Elmerich et al. 1982).
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    Disappearance of plastid and mitochondrial nucleoids during the formation of generative cells of higher plants revealed by fluorescence microscopy (1987)
    Springer
    Protoplasma 141 (1987), S. 149-159 
    Details
    ISSN: 1615-6102
    Keywords: Fluorescence microscopy ; Maternal inheritance ; Plastid nucleoid ; Mitochondrial nucleoid ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The fate of plastid and mitochondrial nucleoids (pt and mt nucleoids) ofTriticum aestivum was followed during the reproductive organ formation using fluorescence microscopy after staining with 4'6-diamidino-2-phenylindole (DAPI). This investigation showed a drastic morphological change of pt nucleoids during the differentiation of reproductive organs from the shoot apex. Dot-shaped pt nucleoids grew into ring-shaped ones, which divided into small pieces in the monocellular pollen grain, as observed in this plant's earlier stage of leaf development. During the development of mature pollen grain from monocellular pollen grain, pt and/or mt nucleoids disappeared through the division of the male generative cell ofT. aestivum. Cytologically, this observation is direct evidence of the maternal inheritance of higher plants. Thus far, cytological evidence of this phenomenon has been found mostly by morphological criteria using electron microscopy, which admits some ambiguity. In the plants exemplified byLilium longiflorum, pt and/or mt nucleoids disappeared after the first pollen grain mitosis, which precededT. aestivum. In the plants exemplified byTrifolium repens, pt and/or mt nucleoids existed in the generative cells of the mature pollen grain. The significance of these observations was discussed in relation to the interaction between nuclear and organelle genomes during plant development.
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    URL: http://dx.doi.org/10.1007/BF01272897
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    Transposon-induced symbiotic mutants of Bradyrhizobium japonicum: Isolation of two gene regions essential for nodulation (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 15-23 
    Details
    ISSN: 1617-4623
    Keywords: Bradyrhizobium japonicum ; Transposon Tn5 ; Mutants ; Nodulation ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two strains of the soybean endosymbiont Bradyrhizobium japonicum, USDA 110 and 61 A101 C, were mutagenized with transposon Tn5. After plant infection tests of a total of 6,926 kanamycin and streptomycin resistant transconjugants, 25 mutants were identified that are defective in nodule formation (Nod-) or nitrogen fixation (Fix-). Seven Nod- mutants were isolated from strain USDA 110 and from strain 61 A101 C, 4 Nod- mutants and 14 Fix- mutants were identified. Subsequent auxotrophic tests on these symbiotically defective mutants identified 4 His- Nod- mutants of USDA 110. Genomic Southern analysis of the 25 mutants revealed that each of them carried a single copy of Tn5 integrated in the genome. Three 61 A101 C Fix- mutants were found to have vector DNA co-integrated along with Tn5 in the genome. Two independent DNA regions flanking Tn5 were cloned from the three nonauxotrophic Nod- mutants and one His-Nod- mutant of USDA 110. Homogenotization of the cloned fragments into wild-type strain USDA 110 and subsequent nodulation assay of the resulting homogenotes confirmed that the Tn5 insertion was responsible for the Nod- phenotype. Partial EcoR1 restriction enzyme maps around the Tn5 insertion sites were generated. Hybridization of these cloned regions to the previously cloned nod regions of R. meliloti and nif and nod regions of B. japonicum USDA 110 showed no homology, suggesting that these regions represent new symbiotic clusters of B. japonicum.
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    Klebsiella pneumoniae nif-lac fusions are expressed in Agrobacterium tumefaciens C58 (1987)
    Springer
    Molecular genetics and genomics 206 (1987), S. 460-464 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium tumefaciens ; Klebsiella pneumoniae ; Nitrogen fixation ; Gene fusions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plasmids containing hybrid genes, in which different Klebsiella pneumoniae nif (nitrogen-fixation) promoters were fused with the structural part of the Escherichia coli lac operon, were introduced into a double auxotrophic derivative of Agrobacterium tumefaciens C58. A study of their expression in the new host was made simple by the inherent inability of A. tumefaciens C58 to produce β-galactosidase unless provided with the wild-type lac operon of E. coli. As shown by quantitative measurements of the enzyme, all K. pneumoniae promoters were expressed well in A. tumefaciens C58, even under conditions known to repress them. It also has been shown that the activity of K. pneumoniae nif A is essential for the expression of nifHDK even when introduced into A. tumefaciens. After entering the new host the plasmids, the nif genes and the fusion alleles contained in them, remained stable. Possible mechanisms responsible for the constitutive behaviour of nif promoters in A. tumefaciens are discussed.
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    URL: http://dx.doi.org/10.1007/BF00428886
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    Sequence of psi, a gene on the symbiotic plasmid of Rhizobium phaseoli which inhibits exopolysaccharide synthesis and nodulation and demonstration that its transcription is inhibited by psr, another gene on the symbiotic plasmid (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 149-154 
    Details
    ISSN: 1617-4623
    Keywords: Beans ; DNA Sequence ; Gene regulation ; Nitrogen fixation ; Nodulation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A gene termed psi (polysaccharide inhibition), located close to the nodulation genes of the Rhizobium phaseoli symbiotic plasmid pRP2JI inhibited exopolysaccharide synthesis (EPS) and nodulation ability (Nod) in Rhizobium when it was cloned in a multicopy plasmid. The sequence of psi showed that it specified a polypeptide of mol. wt. 10000 that may be associated with the membrane of Rhizobium. A second gene, psr (polysaccharide restoration), was located on pRP2JI. When cloned in multicopy plasmids, psr overcame the EPS and Nod defects in strains carrying multicopy psi. Strains with multicopy psr induced non-fixing nodules on Phaseolus beans. Using gene fusions between psi and lacZ, it was shown that psi inhibited transcription of psr.
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    URL: http://dx.doi.org/10.1007/BF00331502
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    Biochemical and genetic analysis of the nifHDKE region of Rhizobium ORS571 (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 280-287 
    Details
    ISSN: 1617-4623
    Keywords: Rhizobium ; Nitrogen fixation ; Nif products ; Tn5 mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Deletions and Tn5 insertions were obtained in a cloned 10 kb BamHI-BglII fragment carrying the nifHDKE region of Rhizobium ORS571 and were recombined into the host genome. Genetic analysis of the mutants, comparison of polypeptides synthesized under conditions of repression and depression of N2 fixation, and biochemical complementation of crude extracts were performed. All Nif- mutants were also Fix-. Three transcription units were identified, nifHDK, nifE and a new nif locus adjacent to nifE; no nif locus was found in the immediate vicinity upstream of nifH. Fifteen polypeptides synthesized under conditions of N2 fixation were characterized by two-dimensional gel electrophoresis. Ten of them are likely to be nif products and polypeptides encoded by nifH, nifD, nifK and tentatively nifE were identified. Physiological and biochemical evidence for the functioning of the second copy of nifH is reported. Nitrogenase component 2 synthesized by this copy could not be differentiated from component 2 synthesized in the wild-type strain. When the function of nifH copy 1 was abolished, the amount of component 2 synthesized was about 30% of that synthesized in the wild-type strain.
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    URL: http://dx.doi.org/10.1007/BF00331590
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    Direct response of Bradyrhizobium japonicum nifA-mediated nif gene regulation to cellular oxygen status (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 621-626 
    Details
    ISSN: 1617-4623
    Keywords: Bradyrhizobium japonicum ; nifA gene ; Nitrogen fixation ; Oxygen control ; Transcriptional control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nifA genes of Klebsiella pneumoniae and Bradyrhizobium japonicum were constitutively expressed from the pBR329-derived chloramphenicol resistance promoter. The inserts of these nifA plasmid constructs were devoid of any other intact flanking genes. The nifA genes thus expressed led to a marked activation of a B. japonicum nifD-lacZ fusion under microaerobic conditions. Under aerobic growth conditions, however, activation was mediated only by the K. pneumoniae nifA gene but not by the B. japonicum nifA gene. This selective effect was observed in both the Escherichia coli as well as the B. japonicum backgrounds. Several lines of evidence suggest that in these experiments oxygen adversely affects B. japonicum nifA-dependent nif gene regulation at the post-transcriptional level, probably even at the post-translational level, and that this effect does not require a nifL-like gene. Models are proposed in which oxygen inhibits the B. japonicum NifA protein either directly or indirectly via other cellular components involved in general protein oxidation pathways.
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    URL: http://dx.doi.org/10.1007/BF00331174
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    A proposed method of polyacrylamide gel electrophoresis in acid environments applied to gliadins of wheat grains (1987)
    Springer
    Euphytica 36 (1987), S. 55-60 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; electrophoresis system ; acid polyacrylamide gel ; gliadins ; variety identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In this paper we describe the method of wheat gliadin electrophoresis in use at RIVRO, Wageningen. It differs from other techniques mainly by the application of an alternative buffer system, making it possible to polymerise the gels in a buffered alkaline environment and to perform the run at pH 3.1 without extensive buffer changing steps. Advantages are a greater gel reproducibility and the ease of gel handling. Furthermore, a rationalised protein extraction procedure, a cheap shaking system for staining baths and a better (slower moving) tracking dye are described.
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    Vernalization of immature embryos of winter wheat genotypes (1987)
    Springer
    Euphytica 36 (1987), S. 161-165 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; winter wheat ; vernalization ; cold treatment ; immature embryos ; excised embryos ; embryo culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of direct vernalization of immature embryos on flowering was studied in six winter wheat genotypes. Fourteen-, 17-, and 20-day-old embryos were excised and vernalized for 0–6 weeks on synthetic medium during a conditioning period. Percent germination of embryos was high (overall 96.1%), and free from genotypic effects. Genotypes differed for flowering in response to cold treatment of excised embryos. Embryo vernalization was as effective as or more than conventional vernalization (control, seedling vernalization for 6 weeks). Seventeen-day-old embryos were the most responsive to vernalization. With a 5-week vernalization of 17-day-old embryos, the percentage of plants anthesed was higher than those from 14-and 20-day-old embryos. For 17-day-old embryos vernalized for 5 weeks, the mean number of days from culture to anthesis was less than that of 6 week vernalization, less than that of 14- and 20-day-old embryos, and less than controls.
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    Variation in high molecular weight glutenin subunits in landraces of hexaploid wheat from Afghanistan (1987)
    Springer
    Euphytica 36 (1987), S. 3-9 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; hexaploid wheat landraces ; variation ; high molecular weight glutenin subunits ; Glu-A1 locus ; Glu-B1 locus ; Glu-D1 locus ; Afghanistan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Variation for high molecular weight (HMW) glutenin subunits is reported in Afghan hexaploid wheat landraces from different locations in the country ranging in altitude from 395 to 3170 metres. The variation appeared to be independent of the altitude and geographical location of the landraces. Studies of a number of samples from each of five sites revealed that at some sites there was allelic variation at theGlu-A1 andGlu-B1 loci coding from HMW glutenin subunits, but there was no variation at theGlu-D1 locus within and between sites.
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    URL: http://dx.doi.org/10.1007/BF00730641
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    Factors affecting haploid production in wheat using the Hordeum bulbosum system. 2. The effect of the timing of pollination (1987)
    Springer
    Euphytica 36 (1987), S. 497-504 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; crossability ; timing of pollination ; frequency of fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of manipulating the timing of pollination on the fertilization frequency in wheat × tetraploid Hordeum bulbosum hybridizations was investigated as a possible means of overcoming varietal incompatibility in this cross. Pre- and post-anthesis pollinations were made on previously identified highly crossable and poorly crossable wheat genotypes and the stage of development of the individual florets at pollination was expressed in ‘days from anthesis’ units. In both crossable and non-crossable wheat genotypes, a significant negative linear relationship was observed between floret age and fertilization frequency. The magnitude of the response of early pollination was dependent on the number of alleles for non-crossability present at the Kr loci. The non-crossable cultivar Highbury, possessing more than one allele for incompatibility, showed a slight response. The greatest response was shown by the highly crossable cultivar Chinese Spring, possessing alleles for crossability at all Kr loci. The single chromosome substitution line, Chinese Spring (Hope 5B), possessing the most potent allele for non-crossability, Kr 1, showed an intermediate response. The H. bulbosum genotype had no effect on the magnitude of this response, influencing only the mean fertilization frequency.
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    URL: http://dx.doi.org/10.1007/BF00041493
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    Factors affecting haploid production in wheat using the Hordeum bulbosum system. 1. Genotypic and environmental effects on pollen grain germination, pollen tube growth and the frequency of fertilization (1987)
    Springer
    Euphytica 36 (1987), S. 483-496 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; pollen grain germination ; pollen tube growth ; frequency of fertilization ; gibberellic acid ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A quantitative examination of pollen grain germination and pollen tube growth within the overy wall was made in selected wheat × tetraploid H. bulbosum hybridizations, to investigate the effect of the wheat and the H. bulbosum genotype on these characters. As expected, variation at the known crossability loci had no effect on pollen grain germination. The frequency of pollen tube penetration of the ovary wall was, however, severely reduced when the dominant alleles were present. Pollen tube penetration was nevertheless observed in 3 of the 110 ovaries examined of the non-crossable cultivar Highbury. The H. bulbosum genotype had a much smaller effect on these characters, but significant differences between the clones were observed in the numbers of pollen tubes initially penetrating the ovary wall. Although two H. bulbosum genotypes showed no significant differences in the number of ovaries with pollen tubes at the base of the ovule, significant differences in the frequency of fertilization were observed. The possible cause of this discrepancy is discussed. The frequency of fertilization in crossable wheat × H. bulbosum hybridizations was improved by the application of gibberellic acid within 10 minutes of pollination, and reduced by an increase in the ambient temperature from 20°C to 26°C. Fertilization following the pollination of non-crossable wheat genotypes was not affected by either of these factors.
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    Factors affecting haploid production in wheat using the Hordeum bulbosum system. 3. Post-fertilization effects on embryo survival (1987)
    Springer
    Euphytica 36 (1987), S. 763-773 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; frequency of fertilization ; seed set ; seed survival ; gibberellic acid ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An investigation was made of factors affecting the frequency of development of fertilized ovules following wheat x Hordeum bulbosum hybridizations. Seed survival, a term used here to represent the relationship between seed set and fertilization, was shown to vary between the wheat genotypes tested, but was unrelated to the crossability genes present within the wheat genotype. A high seed survival was obtained in crosses involving the variety TH3929 and the single chromosome substitution line, Chinese Spring (Hope 5B). Chinese Spring showed a poor seed survival. Some influence of the H. bulbosum genotype on seed survival was detected. The two environmental factors studied, namely the post-pollination application of gibberellic acid and the ambient temperature, were both shown to affect seed survival. The application of gibberellic acid immediately after pollination or 1 and 2 days after pollination improved seed survival, although the extent of the response was dependent upon the number and timing of the application(s). A genotype-dependent response to the ambient temperature was observed, TH3929 showing a slight but insignificant reduction and Chinese Spring a significant improvement in seed survival with an increase in temperature from 20°C to 26°C. The physiological processes which may be influenced by GA and the ambient temperature and may thereby affect seed survival are discussed.
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    Honeycomb selection for yield in early generations of spring wheat (1987)
    Springer
    Euphytica 36 (1987), S. 831-839 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; honeycomb selection ; breeding methodology ; selection intensity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effectiveness of the honeycomb selection method for yield in spring wheat (Triticum aestivum L.) was evaluated using progenies from two wheat crosses, Glenlea x NB131 and Glenlea x Era. Honeycomb selection was carried out in the F2 and F3 generations, grown at the University of Manitoba in the summers of 1980 and 1981, respectively. In both generations, divergent selection was made for both high and low yield. Plants selected in the F3 generation were entered in an F4 yield test in the summer of 1982. Results of the experiment showed that honeycomb selection for yield in the F2 and F3 generations was effective in identifying parents of high- and low-yielding lines. F3 plants from highyielding F2 selections gave higher yields than those from low-yielding F2 selections by 11.5% and 13.0% for Glenlea x NB131 and Glenlea x Era crosses, respectively. The F4 yield test showed that high yielding selections from both crosses significantly outyielded by 8.9% low yielding selections and by 14.4% the unselected composite lines. It is concluded that the honeycomb selection method can be used for early generation selection in spring wheat.
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    Total and CO-reactive heme content of actinorhizal nodules and the roots of some non-nodulated plants (1987)
    Springer
    Plant and soil 100 (1987), S. 225-236 
    Details
    ISSN: 1573-5036
    Keywords: Actinorhizae ; Alnus ; Casuarina ; Frankia ; Hemoglobin ; Myrica ; Nitrogen fixation ; Root nodules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The concentration of total and CO-reactive heme was measured in actinorhizal nodules from six different genera. This gave the upper limit to hemoglobin concentration in these nodules. Quantitative extraction of CO-reactive heme was achieved under anaerobic conditions in a buffer equilibrated with CO and containing Triton X-100. The concentration of CO-reactive heme in nodules of Casuarina and Myrica was approximately half of that found in legume nodules, whereas in Comptonia, Alnus and Ceanothus the concentrations of heme were about 10 times lower than in legume nodules. There was no detectable CO-reactive heme in Datisca nodules, but low concentrations were detected in roots of all non-nodulating plants examined, includingZea mays. Difference spectra of CO treated minus dithionite-reduced extracts displayed similar wavelengths of maximal and minimal light absorption for all extracts, and were consistent with those of a hemoglobin. The concentration of CO-reactive heme was not correlated to the degree to which CO inhibited nitrogenase activity nor was it affected by reducing the oxygen concentration in the rooting zone. However, there was a positive correlation between heme concentration and suberization or lignification of the walls of infected host cells. These observations demonstrate that, unlike legume nodules, high concentrations of heme or hemoglobin are not needed for active nitrogen fixation in most actinorhizal nodules. Nonetheless, a significant amount of CO-reactive heme is found in the nodules of Alnus, Comptonia, and Ceanothus, and in the roots ofZea mays. The identity and function of this heme is unknown.
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    Metabolism and translocation of fixed nitrogen in the nodulated legume (1987)
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    Plant and soil 100 (1987), S. 157-169 
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    ISSN: 1573-5036
    Keywords: Legume ; Nitrogen fixation ; Nodule ; Translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nitrogen (N2) fixed by Rhizobium bacteroids in the legume nodule is excreted as ammonia to the surrounding host cell where it is efficiently assimilated into the amide group of glutamine. Generally glutamine is a minor exported solute of nitrogen, being further metabolised to asparagine in temperate species and to the ureides, allantoin and allantoic acid in tropical species. These solutes serve as the principal translocated forms of nitrogen in xylem. Compartmentalisation of the pathways of nitrogen metabolism and the role of ammonia in regulation of their activity is examined in nodules of both asparagine-forming (Lupinus albus L.) and ureide-forming (Vigna unguiculata L. Walp) symbioses.
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    A test for linkage between Sr6 and Ra in wheat (1987)
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    Euphytica 36 (1987), S. 221-224 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; auricle color ; compact head ; stem rust resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Previous evidence suggested that the gene for red auricle (Ra) might be linked to the gene Sr6 for stem rust resistance on chromosome 2D. However, tests indicated that Ra is not linked to either Sr6 or C, which is also on 2D.
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    Histological studies on the infection of triticale, wheat and rye byPuccinia recondita f.sp.tritici andP. recondita f.sp.recondita (1987)
    Springer
    Euphytica 36 (1987), S. 275-285 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; breadwheat ; T. durum ; durum wheat ; Secale cereale ; rye ; Triticosecale ; triticale ; Puccinia recondita ; leaf rust ; histology ; hypersensitive resistance ; nonhost resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The reaction of eight triticales and of the respective wheat and rye parental lines to infection by the leaf rust fungi of wheat and rye were studied in the seedling stage. The histological observations indicated that wheat and triticale showed a typical nonhost reaction to the leaf rust of rye: sporelings of this fungus were arrested after the formation of primary infection hyphae and before the formation of extensively branched mycelium, mostly without necrosis of plant cells. The rye inbred lines were all susceptible to the rye leaf rust. The reaction of wheat and triticales to the wheat leaf rust was susceptible or resistant. The reaction of resistant lines could be early or late and complete or incomplete, but was associated with substantial necrosis of plant cells, and therefore entirely different from the nonhost reaction to rye leaf rust. In their reaction to wheat leaf rust the rye lines were similar to the resistant wheat and triticale lines. They did not show an important degree of nonhypersensitive early abortion as would be expected in a nonhost species. It appeared that genes for hypersensitive resistance in triticale may be contributed by either the wheat or the rye parental line. A screening of sixty wheat, rye and triticale lines confirmed the nonhost status of wheat and triticale to rye leaf rust and the hypersensitive or moderately susceptible reaction of rye to wheat leaf rust.
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    Crossability percentages of some 1400 bread wheat varieties and lines with rye (1987)
    Springer
    Euphytica 36 (1987), S. 299-319 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Secale cereale ; rye ; crossability ; crossability percentages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two lists are provided: List 1 contains the percentages of crossability with rye of some 1400 varieties and lines of bread wheat; List 2 contains varieties having a high crossability with rye. It is believed that the publication of these data will be helpful to those wishing to cross bread varieties with rye and other species.
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    Long term plant regeneratin from callus cultures of haploid wheat (1987)
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    Plant cell, tissue and organ culture 11 (1987), S. 221-226 
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    ISSN: 1573-5044
    Keywords: Triticum aestivum ; callus induction ; plant regeneration ; somatic embryogenesis ; organogenesis ; inflorescence culture ; haploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus cultures were initiated from young inforescences of 7 haploid pollen plants of wheat. The calli from 3 plants were embryogenic and organogenic. After one and a half years of subculture one callus cultures still retained the ability for high frequency of plant regeneration via somatic embryogenesis and sometimes directly differentiated ovary-like structures. All regenerated plants were haploid having 21 chromosomes.
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    Nitrogen cycling in an Antarctic ecosystem 2. Estimation of the amount of nitrogen fixation in a moss community on East Ongul Island (1987)
    Springer
    Ecological research 2 (1987), S. 31-40 
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    ISSN: 1440-1703
    Keywords: Acetylene reduction ; Antarctica ; Cyanobacteria ; Moss community ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrogen fixation by cyanobacteria in a moss community on East Ongul Island (69°00'S 39°35'E), Antarctica was investigated using the acetylene reduction method. The mean acetylene reduction rate at 10°C and 200 μE·m−2·s−1 photosynthetically active radiation was 7.12 nmol C2H4 per square centimeter of moss community per hour. The effects of temperature, radiation, desiccation and rehydration on the acetylene reduction rates were examined. A simple predictive model was constructed in order to estimate the amount of nitrogen fixed in the field. Using this model, the daily amount of nitrogen fixation was calculated from microclimatic data (temperature and radiation) measured in the experimental field at Syowa Station on East Ongul Island between 1983 and 1984. The cumulative amount of nitrogen fixation in the growing season during this period was estimated to be 329 mg N per square meter of moss community. It is suggested that nitrogen fixation by cyanobacteria in the moss community is important as a nitrogen source for the community growth on East Ongul Island.
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    Field measurement of symbiotic nitrogen fixation in an established lucerne ley using15N and an acetylene reduction method (1987)
    Springer
    Plant and soil 97 (1987), S. 93-104 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Lucerne ; Medicago sativa ; 15N methodology ; Nitrogen fixation ; Quantitative estimation ; Seasonal pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Lucerne is an important forage legume in the south and south-east of Sweden on well-drained soils. However, data is lacking on the apparent amount of nitrogen derived through N2 fixation by field-grown lucerne. This report provides basic information on the subject. The experiment was performed in a lucerne ley grown 40 km north of Uppsala. The input of nitrogen through fixation to the above-ground plant material of an established lucerne (Medicago sativa L.) ley was estimate by15N methodology during two successive years. The amount of fixed N was 242 kg N ha−1 in 1982 and 319 kg N ha−1 in 1983. The proportion of N derived from the atmosphere (%Ndfa) was 70% and 80% for the two years respectively. The first harvest in both years contained a lower proportion fixed N. Both N2 fixation and dry matter production were enhanced during the second year, particularly in the first harvest. The Ndfa was 61% in the first harvest in 1982, compared to 72% Ndfa during the same period in 1983. This demonstrates the strong influence of environment on both dry matter production and N2 fixation capacity of the lucerne. In addition anin situ acetylene reduction assay was used in 1982 to measure the seasonal distribution of the N2 fixation and in 1983 to study the effect of soil moisture on the N2 fixation process. The seasonal pattern showed great dependence on physiological development and harvest pattern of the lucerne ley. The maximum rate of N2 fixation occurred at the bud or early flower stage of growth and was followed by a rapid decline as flowering proceeded. After harvest the nitrogenase activity markedly decreased and remained low during at least two weeks until regrowth of new shoots began. Irrigation doubled the nitrogenase activity of the lucerne in late summer 1983, when soil moisture content in the top soil was near wilting point. No changes in nitrogenase activity did occur in response to watering earlier during the summer, when the soil matric potential was around −0.30 MPa.
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    Effects of delayed soil and foliar N fertilization on yield and N2 fixation of soybean (1987)
    Springer
    Plant and soil 97 (1987), S. 361-368 
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    ISSN: 1573-5036
    Keywords: Foliar fertilization ; Fertilizer uptake efficiency ; Late fertilization ; Nitrogen-15 ; Nitrogen fixation ; Nitrogen translocation ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field experiments were conducted to determine the effects of the amount, time and method of fertilizer N application on the efficiency of N uptake, N2 fixatio and yield of soybean. Soil and foliar fertilizer N, applied during the pod-filling stage were absorbed by plants with equal and high efficiency, compared to an appreciably lower utilization efficiency for N applied before seedling emergence. These results reveal that the soybean roots were active in N uptake during these late stages of growth. Nitrogen fertilization during pod-filling resulted in significant yield increases over the control treatment which received an early application of 20 Kg N/ha. Seed yield increases were, however, more pronounced than total dry matter yield, and virtually all of the late-applied N was translocated into the pods. Nitrogen fixation in soybean was not influenced by the application of 40 kg N/ha to plants as soil or foliar N during the pod-filling stage. However, 80 kg N/ha supplied during pod-filling as 40 kg soil plus 40 kg foliar N/ha significantly reduced the amount of N2 fixed. The results obtained in these studies suggest that inadequate N supply during pod-filling limited soybean yields, and that by the judicious application of fertilizer N during the late stages of growth, it was possible to enhance soybean yields without necessarily inhibiting N2 fixation.
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    URL: http://dx.doi.org/10.1007/BF02383226
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    Use of Azolla as a test organism in a growth chamber of simple design (1987)
    Springer
    Plant and soil 99 (1987), S. 219-230 
    Details
    ISSN: 1573-5036
    Keywords: Azolla ; Blue green alga ; Evapo-transpiratio chamber ; Humidity ; Light condition ; Nitrogen fixation ; Plant environment ; Symbiotic system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The construction and application of a new type of growth chamber, in which different growth conditionsi.e.: temperature, humidity, pH, light intensity, light colour, change in nutrient composition and gas exchange can easily be controlled, are presented. The method has previously been applied to twoAzolla speciesviz. Azolla filiculoides, which is cold tolerant andAzolla pinnata (distinguished in Vietnam as the form Xanh), which is heat tolerant. In the growth chamber natural growth conditions of the Azolla —Anabaena azollae symbiotic association were imitated as much as possible. For testing the system, methods discussed earlier8,14 and some previously presented data, concerning photosynthetic activities, such as oxygen evolution and nitrogen fixation (acetylene reduction) of twoAzolla species39, were partially used. Biomass ofA. filiculoides was measured and reactions to its environment at conditions when grown in the field and in the growth chamber, were studied. Growth and photosynthesis measurements were performed under special light conditions and with whole plants grown under laboratory conditions. Anthocyanin synthesis was studied in relation with humidity. Anthocyanin spectra were analyzed by means of a spectrum-deconvolution method.
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    URL: http://dx.doi.org/10.1007/BF02370869
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    Chromosomal location of dwarfing gene Rht12 in wheat (1987)
    Springer
    Euphytica 36 (1987), S. 521-523 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; plant height ; dwarfing gene ; Rht 12 ; F2 monosomic analysis ; chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chromosomal location of the dwarfing gene Rht12 in the mutant winter wheat Karcagi 522M7K was investigated using F2 monosomic analysis. The segregation ratio for F2 progenies of Chinese Spring monosomics × Karcagi 522M7K, and that of Cheyenne monosomics × Karcagi 522M7K indicated that the near complete dominant dwarfing gene Rht12 is located on chromosome 5A. The heterozygous and hemizygous states of the genes Rht12 have the same effect on plant height.
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    URL: http://dx.doi.org/10.1007/BF00041496
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    Sources of resistance to Mayetiola Destructor in breadwheat and durum wheat in Morocco (1987)
    Springer
    Euphytica 36 (1987), S. 591-602 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; breadwheat ; Triticum turgidum ; durum wheat ; Mayetiola destructor ; Hessian fly ; leaf pubescence ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Grain yield reductions of both breadwheat (Triticum aestivum L.) and durum wheat (Triticum turgidum L. var. durum) caused by attacks of Hessian fly (Mayetiola destructor Say) are second perhaps only to those caused by inadequate soil moisture in Morocco. To identify effective sources of resistance, 817 entries of common wheat and durum wheat reported to be resistant to Hessian fly were evaluated under natural infestations in Morocco. A large number of genes conferring virulence are present in populations of Moroccan Mayetiola. The genes H1, H2, H3, h4, H6, H7, H8, H9, H10, H11, H14, H15, and H16 as well as the ‘Marquillo’, ‘Kawvale’ and PI 94587 resistance sources are not useful for cereal improvement in North Africa. ‘Luso’, which has the gene H12, also appeared susceptible in limited testing. Genotypes having the genes H5 and H13 were identified as significantly reducing larval survial in natural populations of Mayetiola. Of 11 resistant breadwheats identified with unknown genes, seven were from Portugal and three were from the Soviet Union. Although none of the durums tested had high levels of reistance, the two most promising durums were from Portugal. It is proposed that initially H5 be deployed in durum wheats and H13 be used in common wheat improvement. Leaf pubescence appears of little use in reducing the larval survival of Mayetiola.
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    URL: http://dx.doi.org/10.1007/BF00041508
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    The importance of abortive stoma penetration in the partial resistance and nonhost reaction of adult barley and wheat plants to leaf rust fungi (1987)
    Springer
    Euphytica 36 (1987), S. 725-731 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Triticum aestivum ; breadwheat ; Puccinia hordei ; Puccinia recondita f.sp. tritici ; leaf rust ; partial resistance ; nonhost resistance ; adult plant ; stoma penetration ; stomatal exclusion ; histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Early stages of the infection process of Puccinia hordei isolate 1.2.1 and of a P. recondita f.sp. tritici isolate were studied on adult plants of four barley lines and one wheat line. Two of the barley lines are extremely susceptible to P. hordei, the other two have a very high level of partial resistance. A histological study based on a trypan blue staining indicated that stoma penetration by P. hordei isolate 1.2.1 was equally successful on the susceptible as on the partially resistant adult barley plants. Abortion of substomatal vesicles was rare in all lines. These results do not support a hypothesis that mechanisms of partial resistance in adult plants differ from those in seedlings by a substantial abortive stoma penetration. Also in the nonhost combinations wheat-P. hordei and barley-P. recondita f.sp. tritici inhibition of stoma penetration and of substomatal vesicle development appears to play a biologically insignificant role in adult plants. The proportion of stoma penetration on the leaf sheaths of two of the barley lines was as high as on the leaf blades of the flag leaf and the leaf below the flag leaf. There was no evidence for stomatal exclusion as a crucial factor in the relatively low infectibility of leaf sheaths to leaf-blade specialized rust species.
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    URL: http://dx.doi.org/10.1007/BF00051855
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    Electrophoretic variation among and within strains of ‘kharkof’ wheat maintained at 11 locations (1987)
    Springer
    Euphytica 36 (1987), S. 815-822 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; regional nurseries ; natural selection ; genetic drift ; gliadin polyacrylamide gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The hard red winter wheat (Triticum aestivum L.) cultivar Kharkof was introduced to the United States at the turn of the century and has been included as the long-term check for the Southern and Northern Regional Performance Nurseries since 1930. Seed of Kharkof is typically maintained at each site at which the nurseries are grown. We obtained samples of Kharkof from 10 sites and the USDA Small Grains Collection in Beltsville, MD. Up to 54 individual kernels per strain were ground and evaluated for gliadin electrophoretic patterns. Strains from Beltsville, MD and Aberdeen, ID were completely uniform for gliadin patterns; they differed from each other and from all other strains. Seven strains were highly polymorphic, with 7 to 14 gliadin patterns occurring in each. However, there were large differences in pattern frequencies among strains, with the Ft. Collins, CO and Columbia, MO strains at one extreme, the Manhattan, KS and Lincoln, NE strains at the other, and the St. Paul, MN; York, NE; and Clovis, NM strains intermediate. Strains from Dallas and Chillicothe, TX were completely uniform for a pattern that also occurred in all of the polymorphic strains. The two Texas strains also had esterase and β-amylase isoelectric focusing patterns different from the others, and the Beltsville strain had a different β-amylase pattern. We concluded that the strains from Beltsville and Aberdeen were not Kharkof, that the Texas strains were derived from a single-plant selection out of Kharkof, and that the remaining strans have diverged through natural selection possibly operating in conjunction with genetic drift, outcrossing, and seed mixtures.
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    Increase in grain protein percentage in high-yielding common wheat breeding lines by genes from wild tetraploid wheat (1987)
    Springer
    Euphytica 36 (1987), S. 353-359 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; T. turgidum var. dicoccoides ; wild emmer ; nitrogen economy ; grain protein yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Forty-one breeding lines of common wheat, derived from crosses between the Israeli cultivars Miriam and Lakhish and high-protein lines of wild tetraploid wheat, Triticum turgidum var. dicoccoides, were tested for various protein and yield parameters in field trials, under typical agronomic conditions. All lines had a higher grain protein percentage (GPP) than the leading Israeli cultivar Deganit, which was grown as a control. Grain yield (GY) ranged in the breeding lines from a low of 2.44 t/ha to as high as that of Deganit (6.95 t/ha). Despite the weak negative correlation between GPP and GY, several lines excelled both in GPP and in GY. The grain protein yield (GPY) of some of these selected breeding lines was higher than that of Deganit; e.g., 1.19 t/ha in the best line vs. 1.02 t/ha in Deganit. The 16.7% increase in GPY in this line reflected a more efficient utilization of nitrogen.
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    Co-evolution of the legume-Rhizobium association (1987)
    Springer
    Plant and soil 100 (1987), S. 171-181 
    Details
    ISSN: 1573-5036
    Keywords: Co-evolution ; Gene pool ; Nitrogen fixation ; Pisum sativum L. ; Rhizobium leguminosarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A number of examples is given demonstrating the co-existence of pea genotypes and their specific Rhizobium, strains isolated within the same region.R. leguminosarum strains compatible with the cultivated pea have a narrow symbiotic range and they are widely distributed in European soils. This is presumably due to the narrow genetic base of the cultivated pea and its wide-spread cultivation in European soils. Rhizobium strains capable of nodulating a primitive pea line from Afghanistan were only found in soils of the Middle East and Central Asia. A more restricted distribution of specific Rhizobium strains was found for fulvum peas from Israel. Rhizobium strains effective with the fulvum pea were found in Israeli soils. A good example of co-evolution due to geographical isolation was found in south Turkey. Here a pea line was found which can form an effective symbiosis with local Rhizobium strains but not with strains from other parts of Turkey.
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    Endophyte transmission and activity in the Anabaena-Azolla association (1987)
    Springer
    Plant and soil 100 (1987), S. 183-212 
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    ISSN: 1573-5036
    Keywords: Anabaena azollae ; Azolla ; Desiccation ; Macrosporocarps ; Microsporocarps ; Nitrogen fixation ; Sexual reproduction ; Survival Azolla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The survival of Azolla was studied in an artificial system which simulated the soil/water interface and the desiccation of soil during a fallow period in lowland rice culture. Tests with non-sporulating and sporulating Azolla fronds showed that Azolla only survives with sporulated fronds. At their reappearance the Azolla fronds already harboured the Anabaena endophyte. A detailed light microscopic and transmission electron microscopic study of macro- and micros-porocarp formation and development revealed that the endophyte is transmitted by the macrosporocarps and not by the microsporocarps. The Anabaena cells within the macrosporocarps are found just below the indusium cap. These cells are not nitrogen-fixing akinetes. The free-living Anabaena cells at the stem apex and below the overarching developing leaves do not bear heterocysts and accordingly are non nitrogen-fixing. During the development of the leaf the Anabaena enters the leaf cavity, but later the pore of this, cavity closes and the imprisoned cyanobacteria are lysed before the leaf decays. As the Azolla leaves age a nitrogen-fixing capability is successively built up concomittantly with the production of heterocysts. Heterocyst frequencies of 40–50% can be found inAnabaena azollae. Usually a gradient of nitrogen-fixing capacity occurs along the Azolla rhizome with two distinct peaks at leaf number 7/8 and at leaf number 13/14 from the apex.
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    Species variation in the fixation and transfer of nitrogen from legumes to associated grasses (1987)
    Springer
    Plant and soil 98 (1987), S. 265-274 
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    ISSN: 1573-5036
    Keywords: Alfalfa ; Birdsfoot trefoil ; Bromegrass ; Nitrogen fixation ; Nitrogen transfer ; Orchardgrass ; Red clover ; Red fescue ; Tall fescue ; Timothy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three legume species (alfalfa, red clover, and birdsfoot trefoil) in combination with five grass species (timothy, bromegrass, red fescue, tall fescue, and orchardgrass) were used to study N transfer in mixtures, using the 15N dilution technique. The advantage of grass-legume mixtures was apparent. Total herbage and protein yields of grasses in mixtures were higher than those alone, especially at the later cuts. This benefit of mixed cropping is mainly due to N transfer from legumes to associated grasses. N2-fixation and N transfer by alfalfa rated highest, red clover intermediate, and birdsfoot trefoil lowest. The importance of each pathway of N transfer from legumes appeared to differ between species. Alfalfa and red clover excreted more N than trefoil, while the latter contributed more N from decomposition of dead nodule and root tissue. The greatest advantage from a grass-legume mixture, with respect to the utilization of N released from the legume, varied with early maturing tall fescue (Kentucky 31), orchardgrass (Juno), and bromegrass (Tempo), to intermediate timothy (Climax), and least with late maturing red fescue (Carlawn).
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    Nitrogen-fixing (acetylene-reducing) bacteria associated with ectomycorrhizae of Douglas-fir (1987)
    Springer
    Plant and soil 98 (1987), S. 425-428 
    Details
    ISSN: 1573-5036
    Keywords: Diazotrophs ; Ectomycorrhizae ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nitrogenase activity, measured by acetylene reduction, was detected on nursery-grown, surface-sterilized ectomycorrhizae of Douglas-fir, formed withLaccaria laccata, Hebeloma crustuliniforme, Rhizopogon vinicolor, andThelephora sp. Detached mycorrhizae were incubated in nitrogen-free liquid medium under microaerophilic conditions. Nitrogenase activity was attributed toClostridium spp. andAzospirillum spp.
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    Nodulation, nitrogen fixation and nitrogen uptake in pigeonpea (Cajanus cajan (L.) Millsp) of different maturity groups (1987)
    Springer
    Plant and soil 99 (1987), S. 255-266 
    Details
    ISSN: 1573-5036
    Keywords: Cajanus cajan ; Maturity groups ; Nitrogenase activity ; Nitrogen fixation ; Nitrogen uptake ; Nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The seasonal patterns of nodulation, acetylene reduction, nitrogen uptake and nitrogen fixation were studies for 11 pigeonpea cultivars belonging to different maturity groups grown on an Alfisol at ICRISAT Center, Patancheru, India. In all cultivars the nodule number and mass increased to a maximum around 60–80 days after sowing and then declined. The nodule number and mass of medium- and late-maturing cultivars was greater than that of early-maturing cultivars. The nitrogenase activity per plant increased to 60 days after sowing and declined thereafter, with little activity at 100 days when the crop was flowering. At later stages of plant growth nodules formed down to 90 cm below the soil surface but those at greater depth appeared less active than those near the surface. All the 11 cultivars continued to accumulate dry matter until 140 days, with most biomass production by the late-maturing cultivars (up to 11 t ha−1) and least by the early-maturing determinate cultivars (4 t ha−1). Total nitrogen uptake ranged from 69 to 134 kg ha−1. Nitrogen fixation by pigeonpea was estimated as the difference in total nitrogen uptake between pigeonpea and sorghum and could amount to 69 kg N ha−1 per season, or half the total nitrogen uptake. Fixation by pigeonpea increased with crop duration, but there were differences within each maturity group. The limitations of the methods used for estimating N2 fixation by pigeonpea are discussed.
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    A study of nodulation and nitrogen fixation of alder on the purplish soils in China (1987)
    Springer
    Plant and soil 99 (1987), S. 285-290 
    Details
    ISSN: 1573-5036
    Keywords: Alder ; Actinomyces ; Nodulation ; Nitrogen fixation ; Purplish soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The alder has a perennial nodule cluster. The nodule amount on the roots increases with tree age. The N2-fixing activity of nodules decreases with nodule age. Purple coloured soils with various soil pHs and CaCO3 contents are, in the main, the ones which influence nodulation and N2-fixing. Higher N2-fixing capacity existed in the neutral and low calcium soils. High calcium soils and acid soils can restrain nodulation and the N2-fixing rate significantly. On the slope, where calcarous light loams are found, the annual nitrogen fixation capacity of alder and cypress mixed plantations, less than 10 years old, is 16 or 17 kg/ha yr, but in the valley, a pure alder plantation can reach 40 kg/ha yr.
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    Nodulation and symbiotic nitrogen fixation of cowpea (Vigna unguiculata (L.) Walp) (1987)
    Springer
    Plant and soil 99 (1987), S. 435-439 
    Details
    ISSN: 1573-5036
    Keywords: Cowpea ; Effective nodulation ; Nitrogen fixation ; Nodule growth ; Plant age ; Rhizobium strain ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rhizobium strains CIAT 301, CIAT 79 and SLM 602 were tested and found effective in the nodulation and nitrogen fixation of cowpea cv. MI-35 (Vigna unguiculata (L.) Walp) plants in growth chamber experiments. Fresh weight of nodules increased with plant age initially and stabilized in 20–30 days from planting, followed by a secondary flush of nodule growth after 30 days. Apparent nitrogen fixation per gram nodule fresh weight reached a maximum in 20–30 days after planting and then decreased, even though a flush of new nodules was produced.
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    Relevance of randomizing host genotypes of wheat that are tested for leaf rust resistance in race nurseries (1987)
    Springer
    Euphytica 36 (1987), S. 257-263 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f. sp.tritici ; leaf rust ; partial resistance ; race nursery ; hostpathogen interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten wheat leaf rust (Puccinia recondita f.sp.tritici) race nurseries were planted in 1984. In five, each with a different race, no randomization of the 20 wheat genotypes was carried out. The remaining five race nurseries, also each with one of the same five races, were completely randomized. Comparison of the wheat genotype assesments was done using three procedures: i) Correlating (Pearson's and Spearman's r) the leaf area affected of the twenty wheat genotypes in the randomized and the non-randomized nurseries; ii) comparing the best five wheat genotypes in each race nursery; iii) comparing the Wi-indeces of wheat genotypes in the randomized and non-randomized nurseries. All three procedures showed excellent agreement between the randomized and the non-randomized nurseries . The use of a spreader row was most likely the reason for the large similarity. The consequence is that randomization is not sufficiently relevant to justify the increase in risk of mistakes and the extra administration needed. The host genotype-pathogen race interaction was significant and rather large. All but two genotype-race combinations showed a susceptible infection type, which indicates that partial resistance of wheat to wheat leaf rust caused the major part of this interaction. The results which suggest a gene-for-gene relationship, support the \lsintegrated concept\rs of Parlevliet and Zadoks.
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    Induced mutagenesis for the development of multiline cultivars in bread wheat (1987)
    Springer
    Euphytica 36 (1987), S. 49-54 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Puccinia graminis f.sp.tritici ; stem rust ; Puccinia recondita f.sp.tritici ; leaf rust ; Puccinia striiformis ; yellow rust ; races ; induced mutations ; multiline ; resistance breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Variation for resistance toPuccinia graminis f.sp.tritici, P. recondita f.sp.tritici andP. striiformis was induced in theTriticum aestivum cultivar Lalbahadur using nitrosomethyl urea. Variations were isolated from the M2 population in the post-seedling stage in the field when infected with a mixture of races of each of the three rusts. Plants exhibiting simultaneous resistance to stem rust, leaf rust and yellow rust were indentified. Repeated screening in the subsequent generations confirmed the resistance of the mutant lines that are morphologically similar to the parental cultivar. The rust resistance of 20 mutant lines was also confirmed at the seedling stage using individual races of stem rust and leaf rust. The different patterns observed in the mutant lines tested against a wide range of races show that these lines can be used as components of a multiline. The patterns of variation compared with those of the known genes for resistance against the Indian races of the pathogens suggest that the mutations for rust resistance are due to factor different from those already known in bread wheat, providing a broadened genetic base for future breeding programmes.
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    The bread wheat races of bunt represented in the Australian bunt collection (1987)
    Springer
    Euphytica 36 (1987), S. 577-580 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Tilletia laevis ; Tilletia tritici ; common bunt ; physiologic races ; resistance ; Bt genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Australian Bunt Collection, obtained from infected crops between 1962–1977, has been classified into races, based on the differential reactions to ten known bunt resistant genes. Eight physiologic races of Tilletia laevis and three of T. tritici were identified. No race had virulence against the genes Bt3, Bt5, Bt8, or Bt10, of wheat. Consequently four major genes are available for breeding Australian wheat cultivars with resistance to common bunt.
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    Allelopathic effect of parthenium (Parthenium hysterophorus L.) extract and residue on some agronomic crops and weeds (1987)
    Springer
    Journal of chemical ecology 13 (1987), S. 1739-1747 
    Details
    ISSN: 1573-1561
    Keywords: Allelopathy ; parthenium ; Parthenium hysterophorus ; Compositae ; corn ; Zea mays ; ryegrass ; Lolium multiflorum ; wheat ; Triticum aestivum ; velvetleaf ; Abutilon theophrasti ; soybean ; Glycine max ; inhibition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Allelopathic effects of entire shoot extract, plant part extracts, and shoot residue of parthenium (Parthenium hysterophorus L.) on corn (Zea mays L.), ryegrass (Lolium multiflorum Lam.), wheat (Triticum aestivum L.), velvetleaf (Abutilon theophrasti Medik.), and soybean [Glycine max (L) Merr.] growth were examined. Parthenium shoot contained water-soluble materials that were toxic to root growth of velvetleaf and wheat. At 4% (w/ v) concentration, root growth of velvetleaf and wheat were reduced by 60 and 75%, respectively. The order of increasing sensitivity to parthenium was ryegrass, corn, wheat, and velvetleaf. There was a strong correlation between extract concentration and increased toxicity to test species. The toxicity of plant part extracts was also concentration dependent. At 1 and 2% (w/v), the inflorescence and leaves caused more root inhibition than stem extract. Parthenium shoot incorporated in soil at 1% (w/w) caused significantly more root inhibition of wheat than soybean, corn, and ryegrass. At 4% (w/w), root growth of all the test species was inhibited compared to the control. Toxicity of parthenium residue to wheat diminished with increasing periods of decomposition. Residue decomposed for four weeks was less toxic than the undecomposed residue.
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    Infectivity and acetylene reduction of diazotrophic rhizosphere bacteria in wheat (Triticum aestivum) seedlings under gnotobiotic conditions (1985)
    Springer
    Biology and fertility of soils 1 (1985), S. 123-129 
    Details
    ISSN: 1432-0789
    Keywords: Rhizosphere bacteria ; Nitrogenase activity ; Triticum aestivum ; Inoculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat seedlings were inoculated with rhizosphere nitrogen-fixing bacteria and grown gnotobiotically for 15 days. The growth medium consisted of semisolid agar with or without plant nutrients. The bacteria, isolated from roots of field-grown wheat, were three unidentified Gram-negative rods (A1, A2, E1), one Enterobacter agglomerans (C1) and two Bacillus polymyxa (B1, B2). A strain of Azospirillum brasilense (USA 10) was included for comparison. Nitrogenase activity (acetylene reduction activity, ARA) was tested on intact plants after 8 and 15 days of growth. In semisolid agar without plant nutrients, five isolates showed ARA of 0.01–0.9 nmol C2H4 plant−1 h−1, while the two strains of B. polymyxa had higher ARA of 3.3–10.6 nmol C2H4 plant−1 h−1. Plant development was not affected by inoculation with bacteria, except that inoculation with B. polymyxa resulted in shorter shoots and lower root weight. Transmission electronmicroscopy of roots revealed different degrees of infection. A. brasilense, A1 and A2, occurred mainly in the mucilage on the root surface and between outer epidermal cells (low infectivity). B. polymyxa strains and E1 were found in and between epidermal cells (intermediate infectivity) while E. agglomerans invaded the cortex and was occasionally found within the stele (high infectivity).
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    Evidence for adenylylation/deadenylylation control of the glutamine synthetases of Rhodospirillum tenue and Rhodocyclus purpureus (1985)
    Springer
    Archives of microbiology 142 (1985), S. 103-108 
    Details
    ISSN: 1432-072X
    Keywords: Phototrophic bacteria ; Rhodospirillaceae ; Glutamine synthetase ; Nitrogen metabolism ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phylogenetically related phototrophic bacteria Rhodospirillum tenue and Rhodocyclus purpureus modulate activity of their glutamine synthetases by adenylylation/deadenylylation. Evidence for covalent modification includes the inhibitory effect of Mg2+ on the activity of glutamine synthetase extracted from cells of either species grown on excess ammonia, and the lack of Mg2+ inhibition of activity of the enzyme isolated from N2-(R. tenue) or glutamine (R. purpureus)-grown cells. In addition, snake venom phosphodiesterase treatment of glutamine synthetase from either species grown on excess ammonia relieved Mg2+ inhibition of the enzyme (as measured via the γ-glutamyl transferase assay), and changed the cation specificity from Mn2+ to Mg2+ (in the biosynthetic assay).
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    Assimilation of exogenous and dinitrogen-derived13NH 4 + byAnabaena azollae separated fromAzolla caroliniana Willd (1985)
    Springer
    Archives of microbiology 142 (1985), S. 229-233 
    Details
    ISSN: 1432-072X
    Keywords: Ammonium assimilation ; Excretion ; Anabaena azollae ; Azolla caroliniana ; Cyanobacteria ; Glutamine ; Glutamate formation ; Nitrogen fixation ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Anabaena azollae was isolated fromAzolla caroliniana by the “gentle roller” method and differential centrifugation. Incubation of suchAnabaena preparations for 10 min with [13N]N2 resulted in the formation of four radioactive compounds; ammonium, glutamine, glutamate and alanine. Ammonium accounted for 66% of the total radioactivity recovered and 58% of the ammonium was in an extracellular fraction. Since essentially no extracellular13N-labeled organic compounds were found, it appears that ammonium is the compound most probably made available toAzolla during dinitrogen-dependent growth of the association. The kinetics of incorporation of exogenous13NH 4 + into glutamine and glutamate were characteristic of a precursor (glutamine)-product (glutamate) relationship and consistent with assimilation by the glutamine synthetase-glutamate synthase pathway. The results of experiments using the glutamine synthetase inhibitor, methionine sulfoximine, the glutamate synthase inhibitor, diazo-oxonorleucine, and increasing the ammonium concentration to greater than 1 mM, provided evidence for assimilation primarily by the glutamine synthetase-glutamate synthase pathway with little or no contribution from biosynthetic glutamate dehydrogenase. While showing that N2 fixation and NH 4 + assimilation were not tightly coupled metabolic processes in symbioticAnabaena, these results reflect a composite picture and do not indicate the extent to which ammonium assimilatory enzymes might be regulated in filaments associated with specific stages in theAzolla-Anabaena developmental profile.
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    Oxygen protection of nitrogenase in the aerobically nitrogen fixing, non-heterocystous cyanobacterium Oscillatoria sp. (1985)
    Springer
    Archives of microbiology 143 (1985), S. 72-76 
    Details
    ISSN: 1432-072X
    Keywords: Oscillatoria ; Cyanobacteria ; Nitrogen fixation ; Oxygen protection of N2-ase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Oscillatoria sp. strain 23 is a filamentous, non-heterocystous cyanobacterium that fixes nitrogen aerobically. Although, in this organism nitrogenase is inactivated by oxygen a high tolerance is observed. Up to a pO2 of 0.15 atm, oxygen does not have any measurable effects on acetylene reduction. Higher concentrations of oxygen inhibited the activity to a relatively high degree. Evidence for two mechanisms of oxygen protection of nitrogenase in this cyanobacterium was obtained. A high rate of synthesis of nitrogenase may allow the organism to maintain a certain amount of active enzyme under aerobic conditions. Secondly, a switch off/on mechanism may reversibly convert the active enzyme into a non-active form which is insensitive to oxygen inactivation after a sudden and short-term exposure to high oxygen concentrations. It is conceived that these mechanisms in addition to a temporal separation of nitrogen fixation from oxygenic photosynthesis sufficiently explain the regulation process of aerobic nitrogen fixation in this organism.
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    URL: http://dx.doi.org/10.1007/BF00414771
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  • 78
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    Nitrogenase activity in the non-heterocystous cyanobacterium Oscillatoria sp. grown under alternating light-dark cycles (1985)
    Springer
    Archives of microbiology 143 (1985), S. 67-71 
    Details
    ISSN: 1432-072X
    Keywords: Oscillatoria ; Cyanobacteria ; Nitrogen fixation ; Light-dark cycles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The non-heterocystous cyanobacterium Oscillatoria sp. strain 23 fixes nitrogen under aerobic conditions. If nitrate-grown cultures were transferred to a medium free of combined nitrogen, nitrogenase was induced within about 1 day. The acetylene reduction showed a diurnal variation under conditions of continuous light. Maximum rates of acetylene reduction steadily increased during 8 successive days. When grown under alternating light-dark cycles, Oscillatoria sp. fixes nitrogen preferably in the dark period. For dark periods longer than 8 h, nitrogenase activity is only present during the dark period. For dark periods of 8 h and less, however, nitrogenase activity appears before the beginning of the dark period. This is most pronounced in cultures grown in a 20 h light – 4 h dark cycle. In that case, nitrogenase activity appears 3–4 h before the beginning of the dark period. According to the light-dark regime applied, nitrogenase activity was observed during 8–11 h. Oscillatoria sp. grown under 16 h light and 8 h dark cycle, also induced nitrogenase at the usual point of time, when suddenly transferred to conditions of continuous light. The activity appeared exactly at the point of time where the dark period used to begin. No nitrogenase activity was observed when chloramphenicol was added to the cultures 3 h before the onset of the dark period. This observation indicated that for each cycle, de novo nitrogenase synthesis is necessary.
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    Regulation of nitrogenase activity by covalent modification in Chromatium vinosum (1985)
    Springer
    Archives of microbiology 141 (1985), S. 40-43 
    Details
    ISSN: 1432-072X
    Keywords: Nitrogenase ; Nitrogen fixation ; Regulation ; Photosynthetic bacteria ; Chromatium ; Ammonia switch off
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrogenase in Chromatium vinosum was rapidly, but reversibly inhibited by NH 4 + . Activity of the Fe protin component of nitrogenase required both Mn2+ and activating enzyme. Activating enzyme from Rhodospirillum rubrum could replace Chromatium chromatophores in activating the Chromatium Fe protein, and conversely, a protein fraction prepared from Chromatium chromatophores was effective in activating R. rubrum Fe protein. Inactive Chromatium Fe protein contained a peptide covalently modified by a phosphate-containing molecule, which migrated the same in SDS-polyacrylamide gels as the modified subunit of R. rubrum Fe protein. In sum, these observations suggest that Chromatium nitrogenase activity is regulated by a covalent modification of the Fe protein in a manner similar to that of R. rubrum.
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    Electron donation to nitrogenase in heterocysts of cyanobacteria (1985)
    Springer
    Archives of microbiology 143 (1985), S. 185-191 
    Details
    ISSN: 1432-072X
    Keywords: Heterocyst ; Pyruvate: ferredoxin oxidoreductase ; Nitrogen fixation ; Electron transport to nitrogenase ; Ferredoxin ; Cyanobacteria ; Anabaena cylindrica ; Anabaena variabilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Various electron donors were found to stimulate C2H2 reduction (N2 fixation) by isolated heterocysts from Anabaena variabilis and Anabaena cylindrica. Intermediates of glycolysis and the tricarboxylic acid cycle as well as unphosphorylated sugars like glucose, fructose and erythrose were among these electron donors. The transfer of electrons from donors like H2, NADH, glyoxylate and glycollate was strictly light-dependent, whereas others like NADPH or pyruvate plus coenzyme A supported C2H2 reduction also in the dark. In all cases, the overall activity was enhanced by light. The stimulation by light was more distinct with heterocysts from A. variabilis than with heterocysts from A. cylindrica. The present communication establishes that pyruvate supports C2H2 reduction by heterocysts from either A. variabilis or A. cylindrica with rates comparable to those with other electron donors. Pyruvate could, however, support C2H2 reduction only in the presence of coenzyme A, and the concentrations of both coenzyme A and pyruvate were crucial. A pyruvate-dependent reduction of ferredoxin by extracts from heterocysts was recorded spectrophotometrically. Glyoxylate, which is an inhibitor of thiamine pyrophosphate-dependent decarboxylations, inhibited pyruvate-dependent C2H2 reduction. This result supports the conclusion that pyruvate is metabolised by pyruvate: ferredoxin oxidoreductase in heterocysts. High concentrations of pyruvate and other electron donors inhibited C2H2 reduction which suggests that nitrogenase activity in heterocysts may be controlled by the availability of electron donors.
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    Derivatives of Rhizobium meliloti strains carrying a plasmid of Rhizobium leguminosarum specifying hydrogen uptake and pea-specific symbiotic functions (1985)
    Springer
    Archives of microbiology 140 (1985), S. 352-357 
    Details
    ISSN: 1432-072X
    Keywords: Alfalfa ; Conjugation ; Cross inoculation ; Host specificity ; Hydrogen uptake ; Nodulation ; Nitrogen fixation ; Rhizobium ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract pIJ1008, a Rhizobium leguminosarum plasmid which determines hydrogen uptake ability and symbiotic functions in pea was transferable to three of seven natural isolates of R. meliloti tested. In these three strains, pIJ1008 was maintained stably with the respective sym megaplasmid indigenous to each R. meliloti strain. These strains carrying both plasmids nodulated alfalfa but not pea. By reisolation and examination of the strains from alfalfa nodule tissue, it was shown that pIJ1008 continued to be maintained but that pea-nodulation ability was suppressed. In one strain of R. meliloti which carries a 200 kb cryptic plasmid (in addition to a megaplasmid), the transfer and selection for pIJ1008 resulted in the loss of the cryptic plasmid. In three separate plant growth experiments, alfalfa nodules induced by each of the R. meliloti strain carrying both sym plasmids were assayed for hydrogen uptake activity. The average activity was 40-, 3.5-and 2-fold higher than with the respective pIJ1008-free strains. However, this higher activity was not accompanied by an increase in plant biomass or nitrogen content of shoots.
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    Expression ofKlebsiella pneumoniae nif genes inProteus mirabilis (1985)
    Springer
    Archives of microbiology 142 (1985), S. 289-294 
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    ISSN: 1432-072X
    Keywords: Proteus mirabilis ; Nitrogen fixation ; nif genes ; nif plasmids ; Klebsiella pneumoniae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Self-transmissible plasmids carryinghis andnif genes fromKlebsiella pneumoniae have been introduced into threehis mutants ofProteus mirabilis: strains 5006-1, WR19 and WR20. Expression ofhis by the transconjugants was unequivocal, if slightly temperature-sensitive, but none was Nif+ when tested for acetylene reduction in anaerobic glucose medium using inocula from rich or glucose-minimal aerobic agar cultures. Succinate or pyruvate in place of glucose, low glucose, lower temperature or elevated Na2MoO4 did not allownif expression and no nitrogenase MoFe-protein peptide was detected immunologically after exposure to conditions in which diazotrophic enterobacteria, normal or genetically constructed, derepressnif. One strain,P. mirabilis WR19, carrying thehis nif Kmr plasmid pMF250 was examined in detail. Thenif activator genenifA was introduced on the plasmid pCK1. Such derivatives remained Nif- when tested, after aerobic growth on rich agar media, with normal or low glucose, with succinate or with elevated Mo. However, pre-conditioning by aerobic growth on glucose-minimal agar led to subsequent anaerobic expression ofnif in glucose medium from pMF250 in WR19 carrying pCK1. NH 4 + or proline could serve as N-source in the glucose-minimal agar. Maximum activity was about 5% of that ofK. pneumoniae in our assay conditions. Material cross-reacting with anti-serum to the nitrogenase MoFe protein was formed. Nitrogenase activity was not ‘switched off’ by NH 4 + .P. mirabilis WR19 (pCK1) showed NH 4 + -constitutive temperature-sensitive kanamycin resistance (anif-related phenotype of this plasmid) in aerobic glucose minimal medium. Expression ofnif inP. mirabilis WR19 (pCK1, pMF250) was NH 4 + -repressible despite the constitutivenifA character of pCK1 and introduction of thentrA + plasmid pMM17 did not alter this phenotype. However, pCK1 did not give rise to NH 4 + -constitutive diazotrophy in the wild-typeK. pneumoniae M5al. A construct of WR19 carrying pMF250 and constitutiventrC plasmid (pMD45) remained Nif- even after pre-growth on glucose-minimal media. We conclude (a) thatP. mirabilis forms a gene product functionally equivalent to that ofntrA inK. pneumoniae, (b) that it forms no functional equivalent of thentrC product in our growth conditions. The need for pre-conditioning on aerobic glucose media remains perplexing.
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    Concurrent evolution of nitrogenase genes and 16S rRNA in Rhizobium species and other nitrogen fixing bacteria (1985)
    Springer
    Archives of microbiology 142 (1985), S. 342-348 
    Details
    ISSN: 1432-072X
    Keywords: Evolution ; Nif genes ; Nitrogen fixation ; Nitrogenase ; Nucleotide sequence ; Phylogeny ; Rhizobium ; 16S rRNA cataloguing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It was known that nitrogenase genes and proteins are well conserved even though they are present in a large variety of phylogenetically diverse nitrogen fixing bacteria. This has lead to the speculation, among others, that nitrogen fixation (nif) genes were spread by lateral gene transfer relatively late in evolution. Here we report an attempt to test this hypothesis. We had previously established the complete nucleotide sequences of the three nitrogenase genes from Bradyrhizobium japonicum, and have now analyzed their homologies (or the amino acid sequence homologies of their gene products) with corresponding genes (and proteins) from other nitrogen fixing bacteria. There was a considerable sequence conservation which certainly reflects the strict structural requirements of the nitrogenase iron-sulfur proteins for catalytic functioning. Despite this, the sequences were divergent enough to classify them into an evolutionary scheme that was conceptually not different from the phylogenetic positions, based on 16S rRNA homology, of the species or genera harboring these genes. Only the relation of nif genes of slow-growing rhizobia (to which B. japonicum belongs) and fast-growing rhizobia was unexpectedly distant. We have, therefore, performed oligonucleotide cataloguing of their 16S rRNA, and found that there was indeed only a similarity of S AB=0.53 between fast- and slowgrowing rhizobia. In conclusion, the results suggest that nif genes may have evolved to a large degree in a similar fashion as the bacteria which carry them. This interpretation would speak against the idea of a recent lateral distribution of nif genes among microorganisms.
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    Regulation of nitrogenase levels in Anabaena sp. ATCC 33047 and other filamentous cyanobacteria (1985)
    Springer
    Archives of microbiology 141 (1985), S. 105-111 
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    ISSN: 1432-072X
    Keywords: Ammonia ; Anabaena ; Cyanobacteria ; Nitrogen fixation ; Nitrogenase ; Nostoc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Incubation in the dark of photoautotrophically grown N2-fixing heterocystous cyanobacteria leads to a loss of nitrogenase activity. Original levels of nitrogenase activity are rapidly regained upon re-illumination of the filaments, in a process dependent on de novo protein synthesis. Ammonia, acting indirectly through some of its metabolic derivatives, inhibits the light-promoted development of nitrogenase activity in filaments of Anabaena sp. ATCC 33047 and several other cyanobacteria containing mature heterocysts. The ammonia-mediated control system is also operative in N2-fixing filaments in the absence of any added source of combined nitrogen, with the ammonia resulting from N2-fixation already partially inhibiting full expression of nitrogenase. High nitrogenase levels, about two-fold higher than those in normal N2-fixing Anabaena sp. ATCC 33047, are found in cell suspensions which have been treated with the glutamine synthetase inhibitor l-methionine-d,l-sulfoximine or subjected to nitrogen starvation. Filaments treated in either way are insensitive to the ammonia-promoted inhibition of nitrogenase development, although this insensitivity is only transitory for the nitrogen-starved filaments, which become ammonia-sensitive once they regain their normal nitrogen status.
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    Ethylenediamine uptake and metabolism in the cyanobacterium Anabaena variabilis (1985)
    Springer
    Archives of microbiology 141 (1985), S. 244-248 
    Details
    ISSN: 1432-072X
    Keywords: Ammonia analogues ; Anabaena variabilis ; Cyanobacteria ; Ethylenediamine ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cyanobacterium Anabaena variabilis showed a pH dependent uptake of ethylenediamine. No uptake of ethylenediamine was detected at pH 7.0. At higher pH values (e.g. pH 8.0 and pH 9.0) accumulation did occur and was attributed to diffusion of uncharged ethylenediamine in response to a pH gradient. A biphasic pattern of uptake was observed at these higher pH values. Treatment with l-methionine-d,l-sulphoximine (MSX) to inactivate glutamine synthetase (GS) inhibited the second slower phase of uptake without any significant alteration of the initial uptake. Therefore for sustained uptake, metabolism of ethylenediamine via GS was required. NH 4 + did not alter the uptake of ethylenediamine. Ethylenediamine was converted in the second phase of uptake to an analogue of glutamine which could not be detected in uptake experiments at pH 7.0 or in uptake experiments at pH 9.0 following pretreatment of cells with MSX. Ethylenediamine treatment inhibited nitrogenase activity and this inhibition was greatest at high pH values.
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    Denitrification and nitrogen fixation by Azospirillum (1985)
    Springer
    Archives of microbiology 141 (1985), S. 364-370 
    Details
    ISSN: 1432-072X
    Keywords: Nitrogen fixation ; Denitrification ; Associative symbiosis ; Acetylene reduction ; Nitrous oxide formation ; Azospirillum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A model system is described where Azospirillum and germinated wheat seeds were grown in association for a week and then assayed for nitrogen fixation (C2H2-reduction) and denitrification (N2O-formation) activities. The association performed C2H2-reduction and N2O-formation under microaerobic conditions. Both activities were measurable after already 3–5 h of incubation with substantial rates and were strictly dependent on the presence of both plants and bacteria. During the week of the growth of the association, the bacteria had lived exclusively from the carbon compounds supplied by the roots of the plants. C2H2-reduction activity by the association was more or less the same with all the Azospirillum brasilense strains, but lower with A. lipoferum and with the A. amazonense strains tested. Two nitrogenase negative mutants of Azospirillum brasilense showed virtually no activity in the association. C2H2-reduction activity was strongly dependent on the growth temperature of the association. Denitrification (N2O-formation) was high also at higher temperatures and at pH-values in the medium around 7.8 but not at neutrality and was strictly dependent on nitrate. The Azospirillum strain used strongly determined the rate of the N2O-formation in the association. It is suggested that Azospirillum may be beneficial to crops particularly under tropical conditions.
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    Fixation of [13N]N2 and transfer of fixed nitrogen in the Anthoceros-Nostoc symbiotic association (1985)
    Springer
    Planta 164 (1985), S. 406-414 
    Details
    ISSN: 1432-2048
    Keywords: Ammonia/ammonium (assimilation, excretion) ; Anthoceros ; Bryophyta ; Cyanobacteria ; Nitrogen fixation ; Nostoc ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The initial product of fixation of [13N]N2 by pure cultures of the reconstituted symbiotic association between Anthoceros punctatus L. and Nostoc sp. strain ac 7801 was ammonium; it accounted for 75% of the total radioactivity recovered in methanolic extracts after 0.5 min and 14% after 10 min of incubation. Glutamine and glutamate were the primary organic products synthesized from [13N]N2 after incubation times of 0.5–10 min. The kinetics of labeling of these two amino acids were characteristic of a precursor (glutamine) and product (glutamate) relationship. Results of inhibition experiments with methionine sulfoximine (MSX) and diazo-oxonorleucine were also consistent with the assimilation of N2-derived NH 4 + by Anthoceros-Nostoc through the sequential activities of glutamine synthetase (EC 6.3.1.2) and glutamate synthase (EC 1.4.7.1), with little or no assimilation by glutamate dehydrogenase (EC 1.3.1.3). Isolated symbiotic Nostoc assimilated exogenous 13NH 4 + into glutamine and glutamate and their formation was inhibited by MSX, indicating operation of the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway: However, relative to free-living cultures, isolated symbiotic Nostoc assimilated 80% less exogenous ammonium into glutamine and glutamate, implying that symbiotic Nostoc could assimilate only a fraction of N2-derived NH 4 + . This implication was tested by using Anthoceros associations reconstituted with wild-type or MSX-resistant strains of Nostoc incubated with [13N]N2 in the presence of MSX. The results of these experiments indicated that, in situ, symbiotic Nostoc assimilated about 10% of the N2-derived NH 4 + and that NH 4 + was made available to Anthoceros tissue where it was apparently assimilated by the GS-GOGAT pathway. Since less than 1% of the fixed N2 was lost to the suspension medium, it appears that transfer of NH 4 + from symbiont to host tissue was very efficient in this extracellular symbiotic association.
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    Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 31-38 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; 2D electrophoresis ; Gene localization ; Homoeologous genes ; Genetic regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Among the 782 spots observed in two-dimensional gel electrophoresis of denatured proteins from etiolated wheat shoots, 185 were found to be variable between the euploid and 26 ditelosomic lines of ‘Chinese Spring’. Thirty-five structural genes were located on 17 chromosome arms. Numerous intensity changes showing alterations in protein levels were observed and led to the following statements: 1) regulators are frequently found and can be assigned for a same polypeptide to various chromosome arms; 2) for most polypeptides homoeologous arms do not manifest similar effects; 3) nevertheless, when affecting the same polypeptide, homoeologous arms display in most cases identical regulatory effects; 4) gene dosage compensation is observed in only one out of four homoeoallelic situations.
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    Intrachromosomal mapping of the nucleolar organiser region relative to three marker loci on chromosome 1B of wheat (Triticum aestivum) (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 263-270 
    Details
    ISSN: 1432-2242
    Keywords: Intrachromosomal mapping ; Nucleolar organiser region ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction enzyme digestion of the ribosomal RNA genes of the nucleolar organisers of wheat has revealed fragment length polymorphisms for the nucleolar organiser on chromosome 1B and the nucleolar organiser on 6B. Variation between genotypes for these regions has also been demonstrated. This variation has been exploited to determine the recombination frequency between the physically defined nucleolar organiser on 1B (designatedNor1) and other markers; two loci,Glu-B1 andGli-B1 which code for endosperm storage proteins andRf3, a locus restoring fertility to male sterility conditioned byT. timopheevi cytoplasm.Gli-B1 andRf3 were located on the short-arm satellite but recombine with the nucleolar organiser giving a gene order ofNor1 — Rf3 — Gli-B1. Glu-B1 is located on the long arm of 1B but shows relatively little recombination withNor1, which is, in physical distance, distal on the short arm. This illustrates the discrepancy between map distance and physical distance on wheat chromosomes due to the distal localisation of chiasmata. The recombination betweenNor1 andRf3 indicates that, contrary to previous suggestions, fertility restoration is not a property of the nucleolar organiser but of a separate locus.
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    Comparative genetic analyses of metric traits using diallel and factorial mating designs in bread wheat (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 325-328 
    Details
    ISSN: 1432-2242
    Keywords: Line × tester analysis ; Triticum aestivum ; Gene action ; Combining ability ; Mating designs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary For studying the inheritance of metric traits, diallel cross and factorial mating designs are commonly used. Since factorial mating design is less restrictive in crossing plans, the genetic information drawn from it was compared with that from a diallel cross. The comparison was made using graphical, genetic components and combining ability analyses for grain yield, grain weight and spike length in a field experiment of bread wheat (Triticum aestivum L.). Analyses were made on a nine parent diallel cross and a 4 ♀ × 5 ♂ factorial mating design which was sampled from the diallel cross. In general, there was a high degree of agreement between the results obtained from factorial mating design and diallel cross analyses showing thereby that the former provides almost equivalent genetic information to the latter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00662454
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  • 91
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    Genetic control of endosperm proteins in wheat (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 583-589 
    Details
    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Electrophoresis ; Endosperm proteins ; Glutenins ; Gliadins ; Genetic control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Total endosperm proteins extracted from both several common wheat cultivars and some intervarietal substitution lines derived from them were fractionated according to their molecular weight in a high resolution one-dimensional gel electrophoresis. The four donor cultivars and the recipient one — ‘Chinese Spring’, possessed differentially migrating protein bands in the fractions of high molecular weight (HMW) glutenins and gliadins. Several of these bands were identified for the first time in this study. By utilizing intervarietal substitution lines the control of the HMW glutenins and gliadins by chromosomes of homoeologous group 1 was either reaffirmed or, for the new bands, established. Several HMW gliadin subunits showed a considerable variation in their staining intensity in the intervarietal substitution lines indicating that their expression was dependent on the genetic background.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251108
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  • 92
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    Factors affecting the induction of pollen plants of intergeneric hybrids of Triticum aestivum X Triticum-Agropyron (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 294-299 
    Details
    ISSN: 1432-2242
    Keywords: Anther culture ; Pollen callus (plantlet) ; Induction frequency ; Triticum aestivum ; Agropyron glaucum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Experimental results showed that the use of potato extract as a basic component of culture medium had a promoting effect on producing calli in anther culture of the intergeneric hybrids of Triticum aestivum × Triticum-Agropyron (intermediate type). The induction frequencies of pollen callus on the Potato-II medium containing potato extract as the main component was much higher than that found on N6 and W5 media. The induction frequencies of pollen callus and green plantlets in four intergeneric hybrid material inoculated at the late-uninucleate pollen stage were all higher than those inoculated at the mid-uninucleate stage. Appropriate increases in culture temperature significantly increased pollen callus induction frequencies of the intergeneric hybrids. The genotype and physiological state of anther donor plants also influenced pollen callus and green plantlet induction frequencies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304914
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  • 93
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    Structural homology of endosperm high molecular weight glutenin subunits of common wheat (Triticum aestivum L.) (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 634-642 
    Details
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; Triticum aestivum ; Glutenins ; Electrophoresis ; Amino acid analysis ; Peptide mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Several high molecular weight endosperm glutenin subunits, coded by genes located on chromosomes 1A, 1B and 1D of common wheat, Triticum aestivum L. em. Thell., were isolated from excised gel segments and subjected to amino acid analysis and peptide mapping; the latter was carried out following a limited digestion with trypsin, chymotrypsin or Staphylococcus aureus — V8 protease. Generally, all high molecular weight glutenins had a similar amino acid composition but several significant differences were observed in some of them. Both analyses revealed that the structural similarity among the various subunits was related to the homology of the genes coding them: subunits coded by homoalleles, i.e., different alleles of the same gene, were most similar; those coded by homoeoalleles, i.e., alleles of homoeologous genes, were less similar; whereas subunits coded either by alleles of different genes of the same gene cluster, or by nonhomoeoalleles of homoeologous clusters, were the least similar. Several small peptides derived from protease digestion of various subunits had a higher than expected staining intensity indicating that small peptide repeats may be interspersed within the glutenin subunits. The evolutionary course of the high molecular weight glutenins is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252289
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  • 94
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    Nucleolus organizer competition in Triticum aestivum — Aegilops umbellulata chromosome addition lines (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 278-283 
    Details
    ISSN: 1432-2242
    Keywords: Nucleolar competition ; Amphiplasty ; Ag-NORs ; Addition lines ; Triticum aestivum ; Aegilops umbellulata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleolar organizer activity of wheat (Triticum aestivum, AABBDD) and Aegilops umbellulata (UU) chromosomes have been analyzed in the complete set of the chromosome addition lines by using a highly reproducible silver-staining procedure. Chromosomes 1U and 5U produce the partial inactivation of wheat nucleolar organizer chromosomes 6B, 1B and 5D. The chromosomes D and G from Ae. umbellulata, which are not SAT-chromosomes, seem to specifically influence the activity of wheat NORs. The predominant status of the U genome with respect to nucleolar competition in the Triticeae is confirmed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252067
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  • 95
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    Ultrastructural specialization for ureide production in uninfected cells of soybean root nodules (1985)
    Springer
    Protoplasma 125 (1985), S. 1-12 
    Details
    ISSN: 1615-6102
    Keywords: Glycine max ; Root nodule ultrastructure ; Nitrogen fixation ; Peroxisomes (microbodies) ; Uninfected (interstitial) cells ; Ureides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructural studies were conducted on root nodules of soybean (Glycine max) inoculated as seeds withRhizobium japonicum. The development of the large peroxisomes and abundant tubular endoplasmic reticulum (ER) characteristic of the uninfected interstitial cells was followed during nodule growth and maturation. Quantitative data on differences between the uninfected and infected cells in volumes and numbers of peroxisomes, plastids and mitochondria were analyzed statistically. The peroxisomes are 60 times greater in volume per unit cytoplasm in the uninfected cells than the small presumptive peroxisomes in the infected cells. Plastids are about equal in volume in the two types of cells. Mitochondria have 4 × the volume and 3 × the number of profiles per unit cytoplasm in the infected cells than in the uninfected. The observations are discussed in relation to published evidence that several enzymes involved in ureide production are localized in organelles of the uninfected cells. The uninfected cells are viewed as essential components in the symbiotic relationship between host and bacterium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01297345
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  • 96
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    Ultrastructural ontogeny of leaf cavity trichomes inAzolla implies a functional role in metabolite exchange (1985)
    Springer
    Protoplasma 129 (1985), S. 10-27 
    Details
    ISSN: 1615-6102
    Keywords: Azolla ; Anabaena ; Symbiosis ; Nitrogen fixation ; Trichome ; Transfer cell ; Ontogeny ; Ultrastructure ; Gland ; Metabolite exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anabaena azollae is associated with two types of multicellular epidermal trichomes inAzolla leaf cavities, the simple and branched hairs. The observation of transfer cell ultrastructure in some hair cells led to speculation that the cavity hairs might participate in metabolite exchange between the symbionts. The developmental ontogeny of cavity trichomes is described here, using transmission electron microscopy, with a goal of improving our understanding of possible functions of these structures in the symbiosis. The observations have established that all cells of simple and branched hairs develop the structural characteristics of transfer cells, but not simultaneously. Rather, there is an acropetal succession of transfer cell ultrastructure beginning in terminal cells, moving to body cells where present, and ending in stalk cells. The transfer cell stage is followed immediately by senescence in all hair cells. The timing of transfer cell differentiation, considered together with information from other studies, suggests that branched hairs may be involved in exchange of fixed nitrogen between the symbionts, while simple hairs may participate in exchange of fixed carbon fromAzolla toAnabaena.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01282301
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  • 97
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    Transmission of somaclonal variation in wheat (1985)
    Springer
    Euphytica 34 (1985), S. 525-537 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; somaclonal variation ; chromosomal aberrations ; wheat breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Transmission of somaclonal variation was studied in callus derived (SC1) plants of wheat, Triticum aestivum L. Second (SC2), third (SC3) and fourth (SC4) generations were compared with those obtained from embryo culture (E1-E4). SC2 generation had significantly lower grain yield (−37.3%), reduced thousand kernel weight (−7.8%) and shorter height (−2.1%) than that of E2, and included 5.7% seed-sterile and 15.0% partially seed-sterile plants. SC3 generation showed reduced yield (−12.6%), lower thousand kernel weight (−4.9%) and shorter plant height (−1.9%) than E3 generation. SC4 progeny of a short height, partially sterile SC1 variant included aberrant plants: a haploid, few aneuploids, mixoploids, some showing multiploidy, spindle abnormalities, multivalents, bridges and fragments, and tillers having supernumerary spikes with branched rachis. Somaclonal variation resulted from genome instability in callus, and likely involved transposable elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022950
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  • 98
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    The physiology of variation in the time of ear emergence among wheat varieties from different regions of the world (1985)
    Springer
    Euphytica 34 (1985), S. 559-571 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; ear emergence ; number of leaves ; number of spikelets ; photoperiod ; vernalization ; geographical distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Differences in response to photoperiod and vernalization and genetic variation independent of photoperiod and vernalization (earliness per se), affecting time of ear emergence of wheat, were identified in controlled environment experiments with 33 varieties of diverse geographical origin. The results were compared with an analysis of time of ear emergence of 10409 T. aestivum accessions from the USDA Small Grain Collection grown from autumn sowings in Pendleton, Oregon, and spring sowings in Fargo, North Dakota. The effect of differences in photoperiod and vernalization sensitivity on time of ear emergence was similar to the effect of earliness per se, both under controlled environment conditions and in the field. Most of the accessions from low latitude regions reached ear emergence rapidly owing to their insensitivity to photoperiod and vernalization and earliness per se factors accelerating ear emergence. Lateness was common among accessions from Northern Europe, Afghanistan and Turkey, which was due to sensitivity to photoperiod and vernalization, and to earliness per se factors delaying ear emergence. The physiological basis of earliness per se is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022955
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  • 99
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    Plant regeneration from immature inflorescence callus cultures of wheat, rye and triticale (1985)
    Springer
    Euphytica 34 (1985), S. 153-159 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; Triticosecale ; callus culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Callus cultures were initiated from inflorescence explants of wheat, rye and triticale on MS medium supplemented with 2 mgl-1 2,4-D+5% CW or 2 mgl-1 2,4-D+0.5 mgl-1 BA. On transfer of the cultures to medium supplemented with 15% CW+0.2 mgl-1 NAA or 1 mgl-1 BA+0.1 mgl-1 IAA, shoot buds and embryoids were produced. Full fledged plantlets obtained on MS medium supplemented with NAA were transferred to the field. Cytological analysis showed the plants to be diploid. However, the regenerated plantlets were shorter, produced fewer tillers and had lower fertility compared to the control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022875
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  • 100
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    A gene for hybrid necrosis in an inbred line of rye (Secale cereale L.) (1985)
    Springer
    Euphytica 34 (1985), S. 207-211 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; hybrid necrosis ; intergeneric cross
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An inbred line of rye (Secale cereale L.) has been found to carry a gene for hybrid necrosis. This gene was detected in crosses with a highly crossable wheat (Triticum aestivum L.) genotype which carries the gene Ne2. This appears to be the first report of a gene for hybrid necrosis being present in the rye genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022881
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