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  • 1
    Electronic Resource
    Electronic Resource
    The products of Rhizobium genes, psi and pss, which affect exopolysaccharide production, are associated with the bacterial cell surface (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Translational fusions between a mutant phoA (lacking its promoter, ribosomal binding site and signal peptide sequence) and Rhizobium‘symbiotic’ genes were isolated. Since these fusions expressed alkaline phosphatase (AP), the product of phoA, the genes into which phoA was inserted apparently specify proteins located in the bacterial periplasm or cell membrane, the compartment in which AP has activity. These genes were psiA and genes upstream of psiA (psiA is required for normal nodule development and strains with multicopy psiA fall to make exopolysaccharide (EPS) and to nodulate). Fusions between phoA and pss (exo) genes, which are required for EPS production, also resulted in the expression of AP indicating that products of these pss genes were located at the cell surface. Using grus fusions to psiA and pssA, we found that the former was expressed in N2-fixing bean root nodules but the latter was not.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb02140.x
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  • 2
    Electronic Resource
    Electronic Resource
    Discrete differences between strains of different Rhizobium spp. for competitive nodule occupancy on beans (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 681-682 
    Details
    ISSN: 1573-0972
    Keywords: Melanin pigment ; nodulation competitiveness ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Rhizobium etli strain TAL182 and R. leguminosarum bv phaseoli strain 8002, both of which produce melanin pigment, were tested for their nodulation competitiveness on beans by paired inoculation with two strains which do not produce melanin: R. tropici strain CIAT899 and Rhizobium sp. strain TAL1145. An assay was developed to distinguish nodules formed by the melanin-producing and non-producing strains. Strain TAL182 had discrete competitive superiority over CIAT899 and TAL1145 for nodulation of beans. Nodulation competitiveness was not correlated with the ability to produce melanin pigment or the host range of the Rhizobium strains tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00361016
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  • 3
    Electronic Resource
    Electronic Resource
    Improved rate of callus induction from rice anther culture following microscopic staging of microspores in iron alum-haematoxylin (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 95-99 
    Details
    ISSN: 1432-2242
    Keywords: Microspore ; Anther culture ; Haploid production ; Oryza sativa L. ssp. indica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary High frequencies of callusing were obtained in indica rice from the microspores which were staged in acetic acid iron alum-haematoxylin stain prior to culture on G5 medium. Two local varieties, ‘Khonorullo’ and ‘Namyi’, and two advance pre-release cultures, ‘PK 1-1-3’ and ‘PK 12-22’, were used in this investigation. All the cultures exhibited a wide adaptation to varying medium; however, the frequency of callusing was highest (45.5%) in ‘PK 1-1-3’ followed by ‘PK 12-22’ (32.4%) and ‘Khonorullo’ (31.6%). Cold shock (10 °C) for 11 days enhanced the frequency of callusing by 200% in ‘Khonorullo’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290090
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic effect on uptake of selected nutrients in some rice (O. sativa L.) varieties in phosphorus deficient soils (1990)
    Springer
    Plant and soil 123 (1990), S. 117-120 
    Details
    ISSN: 1573-5036
    Keywords: diallel cross ; gene effects ; heterotic effect ; ion uptake ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of genes on the uptake by rice plants of certain macro- and micronutrients (calcium, magnesium, iron, manganese and zinc) was studied, by diallel (7 × 7) analysis, in P deficient upland soil. Both additive and dominant gene effects, with a preponderance of the former, were found to be responsible for the uptake of all the elements studied. Local varieties were found to be not only good yielders but also much more efficient in element uptake. Heterotic effects were observed in various crosses with respect to the uptake of all the aforementioned nutrients. Statistical analysis indicated that while the uptake of iron and zinc were negatively correlated, the uptake of manganese and calcium, manganese and zinc, calcium and magnesium and calcium and zinc were positively correlated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00009935
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  • 5
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of the gene encoding the 33 kDa water oxidizing polypeptide in Anabaena sp. strain PCC 7120 and its expression in Escherichia coli (1989)
    Springer
    Plant molecular biology 13 (1989), S. 427-439 
    Details
    ISSN: 1573-5028
    Keywords: oxygen-evolving complex ; woxA, Anabaena ; manganese-stabilizing polypeptide ; photosystem II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 33 kDa extrinsic polypeptide of photosystem II, also known as the manganese-stabilizing polypeptide (MSP), is located on the lumen side of the thylakoid and is involved in water oxidation. The gene for MSP, designated woxA, has been cloned from the nitrogen-fixing filamentous cyanobacterium Anabaena and sequenced. The woxA open reading frame was found to be 819 bp. The deduced amino acid sequence was 63% and 59% homologous with that of Synechococcus and Synechocystis, respectively, and 44% conserved when compared to the MSP of spinach or pea. Two cysteine residues at positions 48 and 73 were found to be conserved in cyanobacteria and plants. The first 29 amino acids are hydrophobic and may represent the transit peptide. woxA::phoA translational fusion products, in which the body of Escherichia coli alkaline phosphatase was fused to the amino terminal portion of woxA between residues 35 and 130, yielded active alkaline phosphatase in E. coli. Thus the transit peptide of woxA functions in E. coli to transport phosphatase across the cytoplasmic membrane. S1 mapping and primer extension experiments showed that the woxA transcription intitiation site is located 220 bp upstream from the translational start. The woxA promoter has some resemblance to the E. coli consensus and other known Anabaena vegetative cell promoters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015555
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  • 6
    Electronic Resource
    Electronic Resource
    Genetics of silica uptake in selected genotypes of rice (O. sativa L.) (1985)
    Springer
    Plant and soil 88 (1985), S. 449-453 
    Details
    ISSN: 1573-5036
    Keywords: Diallel cross ; Gene action ; Heterotic effect ; Silica uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Silica uptake was studied in dialel cross involving seven genotypes. Maximum profile of this element was found in leaf followed by stem and root. Regarding its uptake both additive and non-additive genes were involved while the former wa higher in magnitude indicating the scope of individual selection in segregating generations. Heterotic effect was also evident. The assessment for efficiency of uptake can be made at an early stage (60 days of growth).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02197502
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  • 7
    Electronic Resource
    Electronic Resource
    Characterization of a Rhizobium etli chromosomal gene required for nodule development on Phaseolus vulgaris L. (1994)
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 583-589 
    Details
    ISSN: 1573-0972
    Keywords: Motility ; ndv gene ; nitrogen fixation ; nodulation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A chromosomal gene, required for nodule development on Phaseolus bean, was characterized from Rhizobium etli strain TAL182. MLC640 is a Tn5 insertion mutant of TAL182 which shows decreased motility in soft TY agar and is defective in nodule development. The site of Tn5 insertion in MLC640 mapped to a 3.6-kb EcoRI chromosomal fragment. The 3.6-kb fragment was subcloned from the cosmid pUHR80 which complemented MLC640. Further subcloning and site-directed Tn5 mutagenesis localized the gene for nodule development to a 1.7-kb region within the 3.6-kb EcoRI fragment. Southern hybridization using the 3.6-kb EcoRI fragment as the probe against genomic DNA of several Rhizobium spp. indicated that this gene is conserved in different rhizobia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00367673
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  • 8
    Electronic Resource
    Electronic Resource
    A mutation that blocks exopolysaccharide synthesis prevents nodulation of peas by Rhizobium leguminosarum but not of beans by R. phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen Xanthomonas (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 320-323 
    Details
    ISSN: 1617-4623
    Keywords: Exopolysaccharide ; Legume ; Nodules ; Rhizobium ; Xanthomonas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A Tn5-induced mutant strain of R. phaseoli which failed to synthesize exopolysaccharide (EPS) was isolated and was shown to induce normal nitrogen-fixing nodules on Phaseolus beans, the host of this Rhizobium species. The corresponding wild-type Rhizobium DNA was cloned in a wide host-range vector and by isolating Tn5 insertions in this cloned DNA, mutations in a gene termed pss (polysaccharide synthesis) were isolated. These were introduced by marker exchange into near-isogenic strains of R. leguminosarum and R. phaseoli which differed only in the identity of their symbiotic plasmids. Whereas the EPS-deficient mutant strain of R. phaseoli induced normal nitrogen-fixing nodules on Phaseolus beans, the same mutation prevented nodulation of peas by a strain of R. leguminosarum which normally nodulates this host. Further, it was found that DNA cloned from the plant pathogen Xanthomonas campestris pathover campestris could correct the defect in EPS synthesis in R. leguminosarum and R. phaseoli and also restored the ability to nodulate peas to the pss::Tn5 mutant strain of R. leguminosarum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333974
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of pss genes of Rhizobium leguminosarum required for exopolysaccharide synthesis and nodulation of peas: Their primary structure and their interaction with psi and other nodulation genes (1988)
    Springer
    Molecular genetics and genomics 213 (1988), S. 155-162 
    Details
    ISSN: 1617-4623
    Keywords: DNA sequence ; Exopolysaccharide ; Nodulation ; psi ; pss ; Rhizobium leguminosarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Strains of Rhizobium leguminosarum (R. l.) biovar viciae containing pss mutations fail to make the acidic exopolysaccharides (EPS) and are unable to nodulate peas. It was found that they also failed to nodulate Vicia hirsuta, another host of this biovar. When peas were co-inoculated with pss mutant derivatives of a strain of R.l. bv viciae containing a sym plasmid plus a cured strain lacking a sym plasmid (and which is thus Nod-, but for different reasons) but which makes the acidic EPS, normal numbers of nodules were formed, the majority of which failed to fix nitrogen (the occasional Fix+ nodules were pressumably induced by strains that arose as a result of genetic exchange between cells of the two inoculants in the rhizosphere). Bacteria from the Fix- nodules contained, exclusively, the strain lacking its sym plasmid. When pss mutant strains were co-inoculated with a Nod- strain with a mutation in the regulatory gene nodD (which is on the sym plasmid pRL1JI), normal numbers of Fix+ nodules were formed, all of which were occupiced solely by the nodD mutant strain. Since a mutation in nodD abolishes activation of other nod genes required for early stages of infection, these nod genes appear to be dispensable for subsequent stages in nodule development. Recombinant plasmids, containing cloned pss genes, overcame the inhibitory effects of psi, a gene which when cloned in the plasmid vector pKT230, inhibits both EPS production and nodulation ability. Determination of the sequence of the pss DNA showed that one, or perhaps two, genes are required for correcting strains that either carry pss mutations or contain multi-copy psi. The predicted polypeptide product of one of the pss genes had a hydrophobic aminoterminal region, suggesting that it may be located in the membrane. Since the psi gene product may also be associated with the bacterial membrane, the products of psi and pss may interact with each other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333413
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  • 10
    Electronic Resource
    Electronic Resource
    Sequence of psi, a gene on the symbiotic plasmid of Rbizobium phaseoli which inhibits exopolysaccharide synthesis and nodulation and demonstration that its transcription is inhibited by psr, another gene on the symbiotic plasmid (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 206-206 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329862
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