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  • Animals  (53)
  • 2000-2004  (53)
  • 1960-1964
  • 2004  (53)
  • 1
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    The Yana RHS site: humans in the Arctic before the last glacial maximum (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-01-06
    Description: A newly discovered Paleolithic site on the Yana River, Siberia, at 71 degrees N, lies well above the Arctic circle and dates to 27,000 radiocarbon years before present, during glacial times. This age is twice that of other known human occupations in any Arctic region. Artifacts at the site include a rare rhinoceros foreshaft, other mammoth foreshafts, and a wide variety of tools and flakes. This site shows that people adapted to this harsh, high-latitude, Late Pleistocene environment much earlier than previously thought.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pitulko, V V -- Nikolsky, P A -- Girya, E Yu -- Basilyan, A E -- Tumskoy, V E -- Koulakov, S A -- Astakhov, S N -- Pavlova, E Yu -- Anisimov, M A -- New York, N.Y. -- Science. 2004 Jan 2;303(5654):52-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for the History of Material Culture, Russian Academy of Sciences, 18 Dvortsovaya nab., St. Petersburg 191186, Russia. pitulko.volodya@nmnh.si.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14704419" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Anthropology ; *Archaeology ; Arctic Regions ; Bone and Bones ; *Cold Climate ; Culture ; Emigration and Immigration ; Geologic Sediments ; Humans ; Paleodontology ; Paleontology ; Plants ; Siberia ; Time
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-10-02
    Description: Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for approximately 20% of global carbon fixation. We report the 34 million-base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand-base pair plastid and 44 thousand-base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Armbrust, E Virginia -- Berges, John A -- Bowler, Chris -- Green, Beverley R -- Martinez, Diego -- Putnam, Nicholas H -- Zhou, Shiguo -- Allen, Andrew E -- Apt, Kirk E -- Bechner, Michael -- Brzezinski, Mark A -- Chaal, Balbir K -- Chiovitti, Anthony -- Davis, Aubrey K -- Demarest, Mark S -- Detter, J Chris -- Glavina, Tijana -- Goodstein, David -- Hadi, Masood Z -- Hellsten, Uffe -- Hildebrand, Mark -- Jenkins, Bethany D -- Jurka, Jerzy -- Kapitonov, Vladimir V -- Kroger, Nils -- Lau, Winnie W Y -- Lane, Todd W -- Larimer, Frank W -- Lippmeier, J Casey -- Lucas, Susan -- Medina, Monica -- Montsant, Anton -- Obornik, Miroslav -- Parker, Micaela Schnitzler -- Palenik, Brian -- Pazour, Gregory J -- Richardson, Paul M -- Rynearson, Tatiana A -- Saito, Mak A -- Schwartz, David C -- Thamatrakoln, Kimberlee -- Valentin, Klaus -- Vardi, Assaf -- Wilkerson, Frances P -- Rokhsar, Daniel S -- New York, N.Y. -- Science. 2004 Oct 1;306(5693):79-86.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉School of Oceanography, University of Washington, Seattle, WA 98195, USA. armbrust@ocean.washington.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15459382" target="_blank"〉PubMed〈/a〉
    Keywords: Adaptation, Physiological ; Algal Proteins/chemistry/genetics/physiology ; Animals ; *Biological Evolution ; Cell Nucleus/genetics ; Chromosomes ; DNA/genetics ; Diatoms/chemistry/cytology/*genetics/metabolism ; *Ecosystem ; Energy Metabolism ; *Genome ; Iron/metabolism ; Light ; Light-Harvesting Protein Complexes/chemistry/genetics/metabolism ; Mitochondria/genetics ; Molecular Sequence Data ; Nitrogen/metabolism ; Photosynthesis ; Plastids/genetics ; Restriction Mapping ; Sequence Alignment ; *Sequence Analysis, DNA ; Silicic Acid/metabolism ; Symbiosis ; Urea/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    Complete genome sequence of the apicomplexan, Cryptosporidium parvum (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-03-27
    Description: The apicomplexan Cryptosporidium parvum is an intestinal parasite that affects healthy humans and animals, and causes an unrelenting infection in immunocompromised individuals such as AIDS patients. We report the complete genome sequence of C. parvum, type II isolate. Genome analysis identifies extremely streamlined metabolic pathways and a reliance on the host for nutrients. In contrast to Plasmodium and Toxoplasma, the parasite lacks an apicoplast and its genome, and possesses a degenerate mitochondrion that has lost its genome. Several novel classes of cell-surface and secreted proteins with a potential role in host interactions and pathogenesis were also detected. Elucidation of the core metabolism, including enzymes with high similarities to bacterial and plant counterparts, opens new avenues for drug development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Abrahamsen, Mitchell S -- Templeton, Thomas J -- Enomoto, Shinichiro -- Abrahante, Juan E -- Zhu, Guan -- Lancto, Cheryl A -- Deng, Mingqi -- Liu, Chang -- Widmer, Giovanni -- Tzipori, Saul -- Buck, Gregory A -- Xu, Ping -- Bankier, Alan T -- Dear, Paul H -- Konfortov, Bernard A -- Spriggs, Helen F -- Iyer, Lakshminarayan -- Anantharaman, Vivek -- Aravind, L -- Kapur, Vivek -- U01 AI 46397/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2004 Apr 16;304(5669):441-5. Epub 2004 Mar 25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Veterinary and Biomedical Science, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA. abe@umn.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15044751" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antiprotozoal Agents/pharmacology ; Carbohydrate Metabolism ; Cryptosporidium parvum/*genetics/*metabolism/pathogenicity/physiology ; DNA, Protozoan/genetics ; Drug Resistance/genetics ; Enzymes/genetics/*metabolism ; Ethanol/metabolism ; Genes, Protozoan ; *Genome, Protozoan ; Glycolysis ; Introns ; Mitochondria/genetics ; Molecular Sequence Data ; Multigene Family ; Open Reading Frames ; Organelles/genetics ; Protozoan Proteins/chemistry/genetics/*metabolism ; Purines/metabolism ; Sequence Analysis, DNA ; Transcription, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    Evidence for addiction-like behavior in the rat (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-08-18
    Description: Although the voluntary intake of drugs of abuse is a behavior largely preserved throughout phylogeny, it is currently unclear whether pathological drug use ("addiction") can be observed in species other than humans. Here, we report that behaviors that resemble three of the essential diagnostic criteria for addiction appear over time in rats trained to self-administer cocaine. As in humans, this addiction-like behavior is present only in a small proportion of subjects using cocaine and is highly predictive of relapse after withdrawal. These findings provide a new basis for developing a true understanding and treatment of addiction.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Deroche-Gamonet, Veronique -- Belin, David -- Piazza, Pier Vincenzo -- New York, N.Y. -- Science. 2004 Aug 13;305(5686):1014-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉INSERM U588, Laboratoire de Physiopathologie des Comportements, Bordeaux Institute for Neurosciences, University Victor Segalen-Bordeaux 2, Domaine de Carreire, Rue Camille Saint-Saens, 33077 Bordeaux Cedex, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15310906" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Behavior, Addictive ; Cocaine/administration & dosage ; *Cocaine-Related Disorders ; Cues ; Electroshock ; Humans ; Male ; Models, Animal ; Motivation ; Motor Activity ; Rats ; Rats, Sprague-Dawley ; Reward ; Self Administration ; Time Factors
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-12-14
    Description: Mammalian oocytes are held in prophase arrest by an unknown signal from the surrounding somatic cells. Here we show that the orphan Gs-linked receptor GPR3, which is localized in the oocyte, maintains this arrest. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype can be reversed by injection of Gpr3 RNA into the oocytes. Thus, the GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle and is crucial for the regulation of meiosis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mehlmann, Lisa M -- Saeki, Yoshinaga -- Tanaka, Shigeru -- Brennan, Thomas J -- Evsikov, Alexei V -- Pendola, Frank L -- Knowles, Barbara B -- Eppig, John J -- Jaffe, Laurinda A -- New York, N.Y. -- Science. 2004 Dec 10;306(5703):1947-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, University of Connecticut Health Center (UCHC), Farmington, CT 06032, USA. lmehlmann@neuron.uchc.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15591206" target="_blank"〉PubMed〈/a〉
    Keywords: Adenylyl Cyclases/metabolism ; Animals ; Chondroitin Sulfate Proteoglycans/genetics/metabolism ; Expressed Sequence Tags ; Female ; Granulosa Cells/physiology ; Heterotrimeric GTP-Binding Proteins/*metabolism ; In Situ Hybridization ; Lectins, C-Type ; Ligands ; Luteinizing Hormone/metabolism ; *Meiosis ; Metaphase ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mitosis ; Oocytes/*physiology ; Ovarian Follicle/*physiology ; RNA/genetics/metabolism ; Receptors, G-Protein-Coupled/genetics/*physiology ; Versicans
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    Rise and fall of the Beringian steppe bison (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-11-30
    Description: The widespread extinctions of large mammals at the end of the Pleistocene epoch have often been attributed to the depredations of humans; here we present genetic evidence that questions this assumption. We used ancient DNA and Bayesian techniques to reconstruct a detailed genetic history of bison throughout the late Pleistocene and Holocene epochs. Our analyses depict a large diverse population living throughout Beringia until around 37,000 years before the present, when the population's genetic diversity began to decline dramatically. The timing of this decline correlates with environmental changes associated with the onset of the last glacial cycle, whereas archaeological evidence does not support the presence of large populations of humans in Eastern Beringia until more than 15,000 years later.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shapiro, Beth -- Drummond, Alexei J -- Rambaut, Andrew -- Wilson, Michael C -- Matheus, Paul E -- Sher, Andrei V -- Pybus, Oliver G -- Gilbert, M Thomas P -- Barnes, Ian -- Binladen, Jonas -- Willerslev, Eske -- Hansen, Anders J -- Baryshnikov, Gennady F -- Burns, James A -- Davydov, Sergei -- Driver, Jonathan C -- Froese, Duane G -- Harington, C Richard -- Keddie, Grant -- Kosintsev, Pavel -- Kunz, Michael L -- Martin, Larry D -- Stephenson, Robert O -- Storer, John -- Tedford, Richard -- Zimov, Sergei -- Cooper, Alan -- New York, N.Y. -- Science. 2004 Nov 26;306(5701):1561-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Henry Wellcome Ancient Biomolecules Centre, Oxford University, South Parks Road, Oxford OX13PS, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15567864" target="_blank"〉PubMed〈/a〉
    Keywords: Alaska ; Animals ; Bayes Theorem ; *Bison/classification/genetics ; Canada ; China ; *Climate ; DNA, Mitochondrial/genetics ; Environment ; *Fossils ; Genetic Variation ; Genetics, Population ; Human Activities ; Humans ; North America ; Phylogeny ; Population Dynamics ; Sequence Analysis, DNA ; Time
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    Neutrophil extracellular traps kill bacteria (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-03-06
    Description: Neutrophils engulf and kill bacteria when their antimicrobial granules fuse with the phagosome. Here, we describe that, upon activation, neutrophils release granule proteins and chromatin that together form extracellular fibers that bind Gram-positive and -negative bacteria. These neutrophil extracellular traps (NETs) degrade virulence factors and kill bacteria. NETs are abundant in vivo in experimental dysentery and spontaneous human appendicitis, two examples of acute inflammation. NETs appear to be a form of innate response that binds microorganisms, prevents them from spreading, and ensures a high local concentration of antimicrobial agents to degrade virulence factors and kill bacteria.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Brinkmann, Volker -- Reichard, Ulrike -- Goosmann, Christian -- Fauler, Beatrix -- Uhlemann, Yvonne -- Weiss, David S -- Weinrauch, Yvette -- Zychlinsky, Arturo -- AI037720/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2004 Mar 5;303(5663):1532-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Microscopy Core Facility, Max Planck Institute for Infection Biology, Schumannstrasse 21/22, 10117 Berlin, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15001782" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Appendicitis/immunology ; Bacterial Proteins/metabolism ; Blood Bactericidal Activity ; Cytochalasin D/pharmacology ; Cytoplasmic Granules/metabolism ; DNA/analysis/metabolism ; Dysentery, Bacillary/immunology ; Endopeptidases/metabolism ; Histones/analysis/metabolism ; Humans ; *Immunity, Innate ; Leukocyte Elastase/analysis/metabolism ; Microscopy, Electron ; *Neutrophil Activation ; Neutrophils/chemistry/*immunology/physiology/ultrastructure ; Phagocytosis ; Rabbits ; Salmonella typhimurium/immunology/*physiology ; Shigella flexneri/immunology/*physiology ; Staphylococcus aureus/immunology/*physiology ; Virulence Factors/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
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    Behavioral ecology. Why male bowerbirds decorate as well as dance (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-04-17
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Morell, Virginia -- New York, N.Y. -- Science. 2004 Apr 16;304(5669):372.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15087514" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Color ; Decision Making ; Female ; Male ; Pigmentation ; *Sexual Behavior, Animal ; *Songbirds/anatomy & histology ; Vocalization, Animal
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
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    Activation of endogenous Cdc42 visualized in living cells (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-09-14
    Description: Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 and other guanosine triphosphatases, the subcellular location of activation is a critical determinant of cell behavior. However, current approaches are limited in their ability to examine the dynamics of Cdc42 activity in living cells. We report the development of a biosensor capable of visualizing the changing activation of endogenous, unlabeled Cdc42 in living cells. With the use of a dye that reports protein interactions, the biosensor revealed localized activation in the trans-Golgi apparatus, microtubule-dependent Cdc42 activation at the cell periphery, and activation kinetics precisely coordinated with cell extension and retraction.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nalbant, Perihan -- Hodgson, Louis -- Kraynov, Vadim -- Toutchkine, Alexei -- Hahn, Klaus M -- GM57464/GM/NIGMS NIH HHS/ -- GM64346/GM/NIGMS NIH HHS/ -- R01 GM057464/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Sep 10;305(5690):1615-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7365, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15361624" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/metabolism ; Algorithms ; Animals ; *Biosensing Techniques ; Cell Adhesion ; Cell Line ; Cell Membrane/*metabolism ; Cell Polarity ; Cell Surface Extensions/metabolism/ultrastructure ; Endothelial Cells/metabolism/ultrastructure ; Fibroblasts ; Fluorescence ; Fluorescent Dyes/chemistry/metabolism ; Green Fluorescent Proteins ; Humans ; Luminescent Proteins ; Mice ; Microtubules/metabolism ; Neutrophil Activation ; Neutrophils/*metabolism ; Proteins/chemistry/metabolism ; Pseudopodia/metabolism ; Pyrimidinones/metabolism ; Sensitivity and Specificity ; Wiskott-Aldrich Syndrome Protein ; cdc42 GTP-Binding Protein/*metabolism ; rho GTP-Binding Proteins/metabolism ; trans-Golgi Network/*metabolism/ultrastructure
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
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    Conservation policy in coffee landscapes (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-01-31
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dietsch, Thomas V -- Philpott, Stacy M -- Rice, Robert A -- Greenberg, Russell -- Bichier, Peter -- New York, N.Y. -- Science. 2004 Jan 30;303(5658):625-6; author reply 625-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14752143" target="_blank"〉PubMed〈/a〉
    Keywords: Agriculture ; Animals ; Coffea/*growth & development ; *Coffee/economics ; Commerce ; *Conservation of Natural Resources ; *Crops, Agricultural/economics ; *Ecosystem ; *Public Policy ; Trees ; United States
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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