ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Immunocytochemistry  (856)
  • wheat  (807)
  • Springer  (1,663)
  • American Chemical Society (ACS)
  • Oxford University Press
Collection
Keywords
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    Microfilaments (F-actin) in generative cells and sperm: an evaluation (1992)
    Springer
    Sexual plant reproduction 5 (1992), S. 89-100 
    Details
    ISSN: 1432-2145
    Keywords: Actin ; Cytoskeleton ; Generative cell ; Immunocytochemistry ; Microtubule ; Mitosis ; Phragmoplast ; Pollen ; Rhodamine phalloidin ; Sperm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Disagreement has arisen over the presence of actin-containing microfilaments (Mfs) in angiosperm generative cells and sperm (GSP). In order to address this issue, we subjected GSP of Tradescantia virginiana, Nicotiana tabacum and Rhododendron laetum to a series of localizations using different antiactins, rhodamine phalloidin and antimyosin. Coordinate staining with antitubulin and Hoechst 33258 defined the status of the microtubule (Mt) cytoskeleton and stages of generative cell division. Additional experiments utilized cytochalasin D (CD). In no instance could Mfs be detected in GSP of the three species. Instead, Mfs seen at the periphery of GSP appear to be continuous with vegetative Mfs and thus are in the vegetative cytoplasm. Mfs are not seen in the constriction zone of dividing T. virginiana generative cells, nor are they indicated in the phragmoplast of N. tabacum and R. laetum. Myosin localizations reveal punctate staining in the vegetative cytoplasm and a thin line of fluorescence around the the outside of the generative cell. While CD seems to delay generative cell division, cytokinesis still takes place. CD-induced Mf fragments are evident in the vegetative cytoplasm but not in GSP. The weight of evidence therefore indicates that GSP do not contain Mfs. The implications of this conclusion for the behavior of GSP and the mechanism of cytokinesis in dividing generative cells are considerable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194867
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Occurrence and expression of acid phosphatase of Hymenoscyphus ericae (Read) Korf & Kernan, in isolation or associated with plant roots (1992)
    Springer
    Mycorrhiza 1 (1992), S. 137-146 
    Details
    ISSN: 1432-1890
    Keywords: Ericaceae ; Mycorrhizal fungi ; Acid phosphatase ; Protein expression ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The activity of acid phosphatase produced in pure culture by the endomycorrhizal fungus Hymenoscyphus ericae (Read) Korf & Kernan (H. ericae LPA 2) was inhibited by high phosphorus levels, alkaline pH, fluoride, molybdate and mannosidase, and activated by concanavalin A. Over 80% of the enzyme activity was due to two wall-bound acid phosphatase isozymes with the characteristics of mannose-rich glycoproteins. Antiserum was raised against the major, low-molecular-weight wall isozyme and its activity tested by immunoblotting and ELISA. The antiserum cross reacted 100% with exocellular (excreted) and 28% with cytoplasmic cellular fractions of H. ericae (LPA 2) cultures, and showed high reactivity with other strains of H. ericae but not with fungal isolates from Erica hispidula L. or E. mauritanica L. Ultrastructural localization of acid phosphatase by cytoenzymology and indirect immunogold labelling confirmed its association with the fungal wall in pure culture and showed that the influence of a high phosphorus level, fluoride and molybdate is through inactivation of the enzyme. Intense acid phosphatase activity, sensitive to the latter inhibitors, was also present on external hyphae growing over a host or non-host root but it was weak or absent from intracellular hyphae where these developed within a host root. Indirect immunolabelling confirmed that this acid phosphatase was of fungal origin and that the specific inhibitory effect of host cells is due to inactivation of the enzyme rather than repression of its synthesis. Possible implications of fungal acid phosphatase in ericoid endomycorrhizal infection processes are discussed together with mechanisms that may be regulating the enzyme activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203287
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The AQP2 water channel: Effect of vasopressin treatment, microtubule disruption, and distribution in neonatal rats (1995)
    Springer
    The journal of membrane biology 143 (1995), S. 165-175 
    Details
    ISSN: 1432-1424
    Keywords: Water channels ; Vasopressin ; Rat kidney ; Immunocytochemistry ; Microtubules ; Cell polarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Aquaporin 2 is a collecting duct water channel that is located in apical vesicles and in the apical plasma membrane of collecting duct principal cells. It shares 42% identity with the proximal tubule/thin descending limb water channel, CHIP28. The present study was aimed at addressing three questions concerning the location and behavior of the AQP2 protein under different conditions. First, does the AQP2 channel relocate to the apical membrane after vasopressin treatment? Our results show that AQP2 is diffusely distributed in cytoplasmic vesicles in collecting duct principal cells of homozygous Brattleboro rats that lack vasopressin. In rats injected with exogenous vasopressin, however, AQP2 became concentrated in the apical plasma membrane of principal cells, as determined by immunofluorescence and immunogold electron microscopy. This behavior is consistent with the idea that AQP2 is the vasopressin-sensitive water channel. Second, is the cellular location of AQP2 modified by microtubule disruption? In normal rats, AQP2 has a mainly apical and subapical location in principal cells, but in colchicine-treated rats, it is distributed on vesicles that are scattered throughout the entire cytoplasm. This is consistent with the dependence on microtubules of apical protein targeting in many cell types, and explains the inhibitory effect of microtubule disruption on the hydroosmotic response to vasopressin in sensitive epithelia, including the collecting duct. Third, is AQP2 present in neonatal rat kidneys? We show that AQP2 is abundant in principal cells from neonatal rats at all days after birth. The detection of AQP2 in early neonatal kidneys indicates that a lack of this protein is not responsible for the relatively weak urinary concentrating response to vasopressin seen in neonatal rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233445
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Peptidergic motoneurons in the buccal ganglia of Aplysia californica Immunocytochemical, morphological, and physiological characterizations (1991)
    Springer
    Journal of comparative physiology 168 (1991), S. 323-336 
    Details
    ISSN: 1432-1351
    Keywords: Aplysia ; Motoneurons ; Immunocytochemistry ; Small cardioactive peptides ; Facilitation ; Depression ; Buccal ; Feeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We used physiological recordings, intracellular dye injections and immunocytochemistry to further identify and characterize neurons in the buccal ganglia of Aplysia calif ornica expressing Small Cardioactive Peptide-like immunoreactivity (SCP-LI). Neurons were identified based upon soma size and position, input from premotor cells B4 and B5, axonal projections, muscle innervation patterns, and neuromuscular synaptic properties. SCP-LI was observed in several large ventral neurons including B6, B7, B9, B10, and B11, groups of s1 and s2 cluster cells, at least one cell located at a branch point of buccal nerve n2, and the previously characterized neurons B1, B2 and B15. B6, B7, B9, B10 and B11 are motoneurons to intrinsic muscles of the buccal mass, each displaying a unique innervation pattern and neuromuscular plasticity. Combined, these motoneurons innervate all major intrinsic buccal muscles (I1/I3, I2, I4, I5, I6). Correspondingly, SCP-LI processes were observed on all of these muscles. Innervation of multiple nonhomologous buccal muscles by individual motoneurons having extremely plastic neuromuscular synapses, represents a unique form of neuromuscular organization which is prevalent in this system. Our results show numerous SCPergic buccal motoneurons with widespread ganglionic processes and buccal muscle innervation, and support extensive use of SCPs in the control of feeding musculature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198352
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Accelerated production of transgenic wheat (Triticum aestivum L.) plants (1996)
    Springer
    Plant cell reports 16 (1996), S. 12-17 
    Details
    ISSN: 1432-203X
    Keywords: Triticum aestivum L. ; wheat ; transformation ; biolistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a method for the accelerated production of fertile transgenic wheat (Triticum aestivum L.) that yields rooted plants ready for transfer to soil in 8–9 weeks (56–66 days) after the initiation of cultures. This was made possible by improvements in the procedures used for culture, bombardment, and selection. Cultured immature embryos were given a 4–6 h pre-and 16 h post-bombardment osmotic treatment. The most consistent and satisfactory results were obtained with 30 μg of gold particles/bombardment. No clear correlation was found between the frequencies of transient expression and stable transformation. The highest rates of regeneration and transformation were obtained when callus formation after bombardment was limited to two weeks in the dark, with or without selection, followed by selection during regeneration under light. Selection with bialaphos, and not phosphinothricin, yielded more vigorously growing transformed plantlets. The elongation of dark green plantlets in the presence of 4–5 mg/l bialaphos was found to be reliable for identifying transformed plants. Eighty independent transgenic wheat lines were produced in this study. Under optimum conditions, 32 transformed wheat plants were obtained from 2100 immature embryos in 56–66 days, making it possible to obtain R3 homozygous plants in less than a year.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275440
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of reserve protein in the endoplasmic reticulum of developing bean (Phaseolus vulgaris) cotyledons (1980)
    Springer
    Planta 150 (1980), S. 419-425 
    Details
    ISSN: 1432-2048
    Keywords: Cotyledons ; Endoplasmic reticulum ; Ferritin labeling ; Immunocytochemistry ; Phaseolus ; Protein (reserve) ; Reserve protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructure of the storage parenchyma cells of the cotyledons of developing bean (Phaseolus vulgaris L.) seeds was examined in ultrathin frozen sections of specimens fixed in a mixture of glutaraldehyde, formaldehyde and acrolein, infused with 1 M sucrose, and sectioned at-80° C. Ultrastructural preservation was excellent and the various subcellular organelles could readily be identified in sections which had been stained with uranyl acetate and embedded in Carbowax and methylcellulose. The cells contained large protein bodies, numerous long endoplasmic reticulum cisternae, mitochondria, dictyosomes, and electron-dense vesicles ranging in size from 0.2 to 1.0 μm. Indirect immunolabelling using rabbit immunoglobulin G against purified phaseolin (7S reserve protein), and ferritin-conjugated goat immunoglobulin G against rabbit immunoglobulin G was used to localize phaseolin. With a concentration of 0.1 mg/ml of anti-phaseolin immunoglobin G, heavy labeling with ferritin particles was observed ober the protein bodies, the cisternae of the endoplasmic reticulum, and the vesicles. The same structures were lightly labeled when the concentration of the primary antigen was 0.02 mg/ml. Ferritin particles were also found over the Golgi bodies. The absence of ferritin particles from other organelles such as mitochondria and from areas of cytoplasm devoid of organelles indicated the specificity of the staining, especially at the lower concentration of anti-phaseolin immunoglobulin G.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390179
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    A segment of rye chromosome 1 enhances growth and embryogenesis of calli derived from immature embryos of wheat (1991)
    Springer
    Plant cell reports 10 (1991), S. 148-151 
    Details
    ISSN: 1432-203X
    Keywords: wheat ; rye ; embryogenesis ; growth ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of the short arm of rye chromosome 1 (1RS) from Secale cereale var. Imperial on the growth and differentiation of callus cultures from wheat Triticum aestivum var. Chinese Spring immature embryos was analysed. This chromosome arm was found to stimulate both embryogenesis and the rate of growth of calli. Recombinant lines carrying segments of 1RS were used to delineate the regions of 1RS responsible for the tissue culture effects. The enhancement of embryogenesis and the stimulation of growth were shown to be associated with two distinct genetic regions of the chromosome arm; the former is located between the centromere and the Sec 1 locus, while the latter is situated in the immediate vicinity of the Sec 1 locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232047
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Subcellular localization of β-1,3-glucanase in Puccinia recondita f.sp. tritici-infected wheat leaves (1998)
    Springer
    Planta 204 (1998), S. 324-334 
    Details
    ISSN: 1432-2048
    Keywords: Key words:β-1 ; 3-Glucanase ; Immunocytochemistry ; Leaf rust pathogen ; Resistance ; Triticum (pathogen resistance)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. An antiserum raised against the purified 33-kDa β-1,3-glucanase of wheat (Triticum aestivum L.) was employed to investigate the ultrastructural localization of the enzyme in wheat leaves infected with Puccinia recondita Rob. ex Desm. f.sp. tritici Eriks. and Henn. using a post-embedding immunogold labelling technique. In both compatible and incompatible interactions, β-1,3-glucanase was detected in the host plasmalemma and in the domain of the host cell wall near the plasmalemma of the mesophyll cells, but higher concentrations of the enzyme were detected in infected resistant wheat leaves than in infected susceptible ones. β-1,3-Glucanase was also found in the secondary thickening of xylem vessels and in the walls of guard cells, epidermal cells and phloem elements, while no labelling was observed in host organelles, viz. vacuoles, mitochondria, endoplasmic reticulum, Golgi bodies, nuclei and chloroplasts. A low concentration of the enzyme was detected on the intercellular hyphal wall and in the hyphal cytoplasm. In the compatible interaction, β-1,3-glucanase was demonstrated to accumulate predominantly in the haustorial wall and extrahaustorial matrix. In the incompatible interaction, strong labelling for β-1,3-glucanase was found in host cell wall appositions, in the extracellular matrix in the intercellular space, and in electron-dense structures of host origin which occurred in the incompatible interaction only.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050263
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    On the cellular localization of phosphoenolpyruvate carboxylase in Sorghum leaves (1981)
    Springer
    Planta 151 (1981), S. 226-231 
    Details
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; (PEP carboxylase) ; PEP carboxylase ; Sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is predominantly located in the cytoplasm of mesophyll cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395173
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of phaseolin and phytohemagglutinin in the endoplasmic reticulum and Golgi complex of developing bean cotyledons (1985)
    Springer
    Planta 164 (1985), S. 295-302 
    Details
    ISSN: 1432-2048
    Keywords: Cotyledon ; Golgi complex ; Immunocytochemistry ; Phaseolus (seed proteins) ; Phaseolin ; Phytohemagglutinin ; Protein (seeds)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Development of legume seeds is accompanied by the synthesis of storage proteins and lectins, and the deposition of these proteins in protein-storage vacuoles (protein bodies). We examined the subcellular distribution, in developing seeds of the common bean, Phaseolus vulgaris L., of the major storage protein (phaseolin) and the major lectin (phytohemagglutinin, PHA). The proteins were localized using an indirect immunocytochemical method in which ultrathin frozen sections were immunolabeled with rabbit antibodies specific for either PHA or phaseolin. Bound antibodies were then localized using goat-anti-rabbit immunoglobulin G adsorbed onto 4- to 5-nm colloidal gold particles. The sections were post-fixed with OsO4, dehydrated, and embedded in plastic on the grids. Both PHA and phaseolin exhibited a similar distribution in the storage-parenchyma cells, being found primarily in the developing protein bodies. Endoplasmic reticulum and Golgi complexes (cisternal stacks and associated vesicles) also were specifically labeled for both proteins, whereas the cytosol and other organelles, such as mitochondria, were not. We interpret these observations as supporting the hypothesis that the transport of storage proteins and lectins from their site of synthesis, the rough endoplasmic reticulum, to their site of deposition, the protein bodies, is mediated by the Golgi complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402940
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 11
    Electronic Resource
    Electronic Resource
    The major albumin protein from pea (Pisum sativum L.) (1985)
    Springer
    Planta 165 (1985), S. 522-526 
    Details
    ISSN: 1432-2048
    Keywords: Albumin (localisation) ; Cotyledon ; Pisum (albumin protein) ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The major albumin protein in storage parenchyma tissue of developing peas has been localised at an ultrastructural level by immunocytochemistry. Tissue was fixed in buffered aldehyde and embedded in LR White resin which was polymerised by addition of catalyst. Sections were labelled by the indirect method of absorption of Protein A-gold to specifically bound antibodies. This method gives high levels of specific labelling on sections which retain good ultrastructural preservation and have high contrast after conventional staining. The albumin is located throughout the cytoplasm although no labelling was found associated with the endoplasmic reticulum, Golgi apparatus, vacuoles-protein bodies or other organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00398098
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Immunochemical studies on xanthine dehydrogenase of soybean root nodules (1986)
    Springer
    Planta 167 (1986), S. 190-195 
    Details
    ISSN: 1432-2048
    Keywords: Glycine (xanthine dehydrogenase) ; Immunocytochemistry ; Polyclonal antibody ; Root nodule ; Xanthine dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Xanthine dehydrogenase (XDH, EC 1.2.1.37) was purified from root nodules of soybean (Glycine max) and used to prepare a polyclonal rabbit antiserum. Monospecificity of this antiserum was ascertained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipate. During root nodule development of soybean, only one form of XDH was detected on an immunological basis. Titration of XDH by immunoelectrophoresis showed that a remarkable increase in the amount of XDH occurred between two and four weeks after inoculation, in parallel with the increase in enzyme activity. Localization of XDH by immunofluorescence indicated that the enzyme was present exclusively in uninfected cells where it appeared to be associated with discrete organellels
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391414
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Opsin localization and chromophore retinoids identified within the basal brain of the lizard Anolis carolinensis (1993)
    Springer
    Journal of comparative physiology 172 (1993), S. 33-45 
    Details
    ISSN: 1432-1351
    Keywords: Photoreception ; Extraretinal Photoreceptor ; Chromophore ; Opsin ; Reptile ; Immunocytochemistry ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Since the beginning of this century evidence has accumulated which demonstrates that non-mammalian vertebrates possess photoreceptors situated deep within the brain. While many attempts have been made to localize these sensory cells, studies have either failed or been inconclusive. In this report we have used several experimental approaches to localize the deep brain photoreceptors of the lizard Anolis carolinensis. Using 3 antibodies that bind vertebrate cone opsins, we have immunolabelled cerebrospinal fluid (CSF)-contacting neurons located at the ventricular border within the nucleus ventromedialis of the septum. Western blot analysis indicates that these antibodies recognized a single 40 kD protein in ocular, anterior brain, and pineal extracts. Immunoblots of rodent brain did not show a similar protein band. We have also identified specific retinoids associated with phototransduction (11-cis and all-trans-3,4-didehydroretinaldehyde) within anterior brain extracts. This combined data provides the most detailed analysis of deep brain photoreceptors in any vertebrate. Consequently, we feel Anolis provides an excellent model to study this unexplored sensory system of the vertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214713
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    Isolation of an egg-laying hormone-binding protein from the gonad of Aplysia californica and its localization in oocytes (1993)
    Springer
    Journal of comparative physiology 173 (1993), S. 475-483 
    Details
    ISSN: 1432-1351
    Keywords: Egg laying hormone ; Aplysia ; Binding protein ; Immunocytochemistry ; Reproductive system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A protein solubilized from a membrane preparation of the gonad of Aplysia californica has been isolated by affinity chromatography, using bag cell egg-laying hormone (ELH) as the bound ligand, and partially purified and characterized by gel electrophoresis. The protein has an apparent molecular weight of 52 kDa and consists of two disulfide-linked subunits of about 30 kDa each. The protein is glycosylated and has an acidic pI. Approximately 10–15 μg of this protein can be isolated from a single ovotestis, representing less than 1% of the total protein in the gonad; but the protein could not be detected in buccal mass or body wall, tissues which do not have apparent response to ELH. Antibodies generated against this ELH-binding protein (ELHBP) were used to localize sites in the ovotestis which might contain this molecule and thus represent targets for egg-laying hormone. Immunocytochemical results indicate that the oocytes are a rich source of this protein, since their cytoplasm was the only detectable site of immunoreactivity. Whether this binding protein represents an egg-laying hormone receptor is uncertain, but its prevalence in oocytes suggests that ELH plays a signaling role on these gametes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193520
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    Extracellular localization of cathepsin D in ossifying cartilage (1973)
    Springer
    Calcified tissue international 12 (1973), S. 313-321 
    Details
    ISSN: 1432-0827
    Keywords: Cathepsin D ; Extracellular ; Ossification ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé De la cathepsine D extracellulaire d'origine principalement ostéoblastique a été démontrée par immunohistochimie dans le cartilage en voide de calcification de cultures d'os des membres d'embryons de poulet. L'intérêt de ce fait pour l'ossification est discuté.
    Abstract: Zusammenfassung Es wurde mit einer immunohistochemischen Methode gezeigt, daß extrazelluläres Kathepsin D, welches hauptsächlich aus Osteoblasten gewonnen wurde, Knorpel von kultivierten Knochen von Kükenembryonen mineralisiert. Die Bedeutung dieser Feststellung für den Mechanismus der Knochenbildung wird diskutiert.
    Notes: Abstract Extracellular cathepsin D derived mainly from osteoblasts has been demonstrated immunohistochemically in ossifying cartilage of cultured embryonic chick limb bones. The relevance of this observation to the mechanism of ossification is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02013744
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    Immunocytochemical identification of androgen receptor in mouse osteoclast-like multinucleated cells (1994)
    Springer
    Calcified tissue international 54 (1994), S. 325-326 
    Details
    ISSN: 1432-0827
    Keywords: Androgen Receptor ; Osteoclast ; Mouse ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Expression of androgen receptor (AR) in mouse osteoclast-like multi-nucleated cells (OCs) was examined with immunocytochemical techniques. Murine OCs were obtained by co-culturing mouse osteoblastic cells and bone marrow cells. Three preparations of polyclonal anti-AR antibody which were raised in rabbit against different parts of the human AR were employed for the experiments. Specific staining for AR was demonstrated in the nuclei and the perinuclear area of mouse OCs. This is the first report demonstrating the presence of AR in osteoclast-like cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295958
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Expression of growth hormone receptor by immunocytochemistry in rat molar root formation and alveolar bone remodeling (1992)
    Springer
    Calcified tissue international 50 (1992), S. 541-546 
    Details
    ISSN: 1432-0827
    Keywords: Growth hormone ; Growth hormone receptor ; Odontogenesis ; Bone remodeling ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Growth hormone (GH) may regulate tooth formation and bone remodeling associated with tooth eruption. This study reports the distribution of growth hormone receptor/binding protein in developing rat molars and adjacent alveolar bone by immunocytochemistry using well-characterized anti-growth hormone receptor monoclonal antibodies. These tissues represent an excellent model for studying the ontogenic changes that occur in odontogenic and osteogenic cells, as these cells are found in linear arrays displaying the various stages of morphological and functional differention, and differentiated function. Immunoreactivity was first seen in precementoblasts in contact with the epithelial root sheath, and preodontoblasts. However, growth hormone receptor immunoreactivity was associated primarily with the cytoplasm of odontogenic and osteogenic cells forming their respective matrices. Thus, cementoblasts and odontoblasts at sites of new matrix formation showed intense immunoreactivity whereas cementocytes and mature odontoblasts at later stages of tooth development were nonreactive. Osteoblasts engaged in intramembranous ossification in the alveolar bone were positive, although osteocytes and endosteal cells were immunonegative. Osteoclasts at sites of alveolar bone remodeling resorption were also immunopositive. These patterns of receptor expression parallel the ontogenic sequences of odontogenic and osteogenic cells and suggest that GH promotes the functional state of these cells. Our results also imply that GH may influence differentiation or differentiated functions associated with odontogenesis, osteogenesis, and bone remodeling independent of systemic insulin-like GF-I.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582170
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Effect of feeding cereal-legume diets to rats on in vitro absorption of45Ca (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 1016-1017 
    Details
    ISSN: 1420-9071
    Keywords: In vitro absorption ; calcium ; wheat ; Bengal gram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The in vitro absorption of calcium from the duodenum was significantly less in a group of rats fed on a wheat diet than in a group fed a wheat and Bengal gram (70∶30) diet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01940659
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    DNA in wheat seeds from European archaeological sites (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 571-575 
    Details
    ISSN: 1420-9071
    Keywords: Ancient DNA ; archaeobotany ; carbonized grain ; DNA sequences ; glutenin alleles ; seed proteins ; Triticum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have used hybridization analysis to detect ancient DNA in wheat seeds collected from three archaeological sites in Europe and the Middle East. One of these samples, carbonizedT. spelta dated to the first millennium BC, has yielded PCR products after amplification with primers directed at the leader regions of the HMW (high molecular weight) glutenin alleles. Sequences obtained from these products suggest that the DNA present in the Danebury seeds is chemically damaged, as expected for ancient DNA, and also indicate that it should be possible to study the genetic variability of archaeological wheat by ancient DNA analysis. Finally, we describe a PCR-based system that enables tetraploid and hexaploid wheats to be distinguished.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01921727
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    Occurrence, distribution and nature of neuropeptide Y in the rat pancreas (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1554-1557 
    Details
    ISSN: 1420-9071
    Keywords: Immunocytochemistry ; neuropeptide Y ; radioimmunoassay ; rat pancreas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Significant quantities of a newly discovered peptide, neuropeptide Y, were found in the rat pancreas, where they were localized to nerves in the exocrine parenchyma and around arterial and ductal structures. Although unaffected by surgical parasympathectomy, the periarterial and periductal nerves were abolished by chemical sympathectomy, suggesting that NPY is partially costored with sympathetic transmitters in nerve fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01964804
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 21
    Electronic Resource
    Electronic Resource
    Immunocytochemical demonstration of calmodulin in cells secreting by exocytosis (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1340-1342 
    Details
    ISSN: 1420-9071
    Keywords: Immunocytochemistry ; calmodulin ; secretory granules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Calmodulin is a regulator of several calcium-dependent cellular processes. It has been suggested that it plays a role in the mechanism of secretion. Employing an indirect immunoperoxidase technique at the light microscope level, this study demonstrates the presence of calmodulin in several exocytotic cells (mast cells, thyroid follicular cells, neurohypophyseal neurosecretory terminals, pancreaticβ-cells and pancreatic acinus cells) in rat and man. The positive staining reaction for calmodulin was granular and at least in the case of rat mast cells it appeared to be associated with the granule membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01952085
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 22
    Electronic Resource
    Electronic Resource
    Effects of 2-β-D-glucosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one onSchizaphis graminum (rondani) (insecta, aphididae) feeding on artificial diets (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 514-516 
    Details
    ISSN: 1420-9071
    Keywords: Hydroxamic acid ; cereals ; maize ; wheat ; aphids ; greenbug
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 2-β-Glucosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA-Glc), the main hydroxamic acid from maize and wheat, and its aglucone, decreased survival ofSchizaphis graminum reared on artificial diets. Both compounds were toxic for aphids and acted as feeding deterrents, at concentrations as low as 1 mM. The natural concentrations of glucosides of hydroxamic acids in wheat leaves reach up to 6 mmoles/kg fresh weight, thus falling within the range in which DIMBOA-Glc causes delecterious effects to diet-fed aphids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01966177
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 23
    Electronic Resource
    Electronic Resource
    Immunocytochemical location of vitellin in the egg of the silkworm,Bombyx mori, during early developmental stages (1983)
    Springer
    Development genes and evolution 192 (1983), S. 113-119 
    Details
    ISSN: 1432-041X
    Keywords: Vitellin ; Yolk granule ; Yolk protein ; Silkworm ; Embryogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vitellin was purified from eggs of the silkworm,Bombyx mori, by a new method in which vitellin was extracted from isolated yolk granules. The purified vitellin had a molecular weight of 540,000. An antibody against purified vitellin was prepared in rabbits. It reacted with the hemolymph vitellogenin as well as with purified vitellin, but not with other proteins in the hemolymph or in the extract from yolk granules. The anti-vitellin IgG was used to immunocytochemically locate vitellin in theBombyx non-diapause egg during early developmental stages. In the egg, just after oviposition, vitellin was located in internal yolk granules and in small yolk granules of the periplasm. During the early developmental stages studied, vitellin was not metabolized uniformly throughout the egg. The vitellin of the internal yolk granules located at the posterior-dorsal part and of the small peripheral yolk granules was utilized in 16 h and 2 days, respectively, after oviposition. A thin, very vitellin-poor layer was located between the periplasm and the vitellin-rich interior in the newly laid egg. it was always in close contact with the periphery where blastoderm and germ-band cells developed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848679
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 24
    Electronic Resource
    Electronic Resource
    Immunocytochemical and electrophoretic studies on the localization of the major haemolymph proteins (ceratitins) inCeratitis capitata during development (1984)
    Springer
    Development genes and evolution 194 (1984), S. 37-43 
    Details
    ISSN: 1432-041X
    Keywords: Major haemolymph proteins ; Development ; Cuticle ; Immunocytochemistry ; Ceratitis capitata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental profile of the major haemolymph proteins (ceratitins) inCeratitis capitata was studied. Ceratitin concentration in the haemolymph decreases dramatically during the last days of pupal life, while the amounts of ceratitins in whole organism extracts remain unchanged. By electrophoretic, immunological and immunofluorescence techniques it was revealed that ceratitins are reabsorbed by the fat body and a fraction of them is deposited in the cuticle. The possible role of ceratitins is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848952
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 25
    Electronic Resource
    Electronic Resource
    The egg-jelly macromolecule, a fucose sulphate glycoconjugate, originates from the accessory cells of the ovary in the sea urchin Hemicentrotus pulcherrimus (1992)
    Springer
    Development genes and evolution 201 (1992), S. 179-189 
    Details
    ISSN: 1432-041X
    Keywords: Sea urchin ; Jelly coat ; Accessory cell ; Oogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The immunocytochemical localization of the egg-jelly macromolecule, a fucose sulphate glycoconjugate (FSG) that induces the acrosome reaction in spermatozoa, was investigated in ovaries of the sea urchin Hemicentrotus pulcherrimus by use of a polyclonal antibody. The polyclonal antibody reacted with the accessory cells and oocytes in the ovarian lumen. In the accessory cells, evidence of an intense immunohistochemical reaction was observed in many globules of variable density. Products of the specific immunohistochemical reaction were frequently observed in the surface region of oocytes, at a distance from the ovarian wall. At the ultrastructural level, the polyclonal antibody was found to react with the material present in the vacuole-like structures of the globules in the accessory cells. Many gold particles, demonstrating specific immunolabelling, were associated with well-developed microvilli on the vitellogenic oocytes. In the mature oocytes, intense labelling was observed in the jelly coat but not in the vitelline coat. By contrast, oogonia and early oocytes were barely labelled. Quantitative data indicated that the extent of immunolabellings in the surface region of oocytes was very high in the vitellogenic and mature oocytes. In all cases, neither the oocyte cytoplasm nor the subcellular organelles were labelled. These results suggest that FSG is produced by the accessory cells and is deposited initially on the surface of vitellogenic oocytes for the formation of jelly. These findings may provide a new insight into the role of the accessory cells in the reproductive process of the sea urchin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00188717
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 26
    Electronic Resource
    Electronic Resource
    Seasonal variations in the production of the egg-jelly macromolecule, a fucose sulphate glycoconjugate, by the accessory cells in the ovary of the sea urchin Hemicentrotus pulcherrimus (1994)
    Springer
    Development genes and evolution 203 (1994), S. 402-410 
    Details
    ISSN: 1432-041X
    Keywords: Sea urchin ; Egg jelly ; Ovary ; Development ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the sea urchin Hemicentrotus pulcherrimus, the egg-jelly macromolecule, a fucose sulphate glycoconjugate (FSG) that induces the acrosome reaction in spermatozoa, originates from the accessory cells in the ovary. In the present study we examined the seasonal variations in the distribution of FSG in the ovary by immunocytochemistry with a polyclonal antibody. An enzyme-linked immunosorbent assay indicated that FSG was present in supernatants of extracts of ovaries throughout the development of the ovary. However, the immunohistochemical study showed that there are marked seasonal changes in the distribution of FSG in ovaries. The polyclonal antibody reacted strongly with globules of accessory cells before the beginning of the breeding season (August to December). During the breeding season (February to April), the immunohistochemical reaction was found on the surface of oocytes but was weak in the accessory cells. At the ultrastructural level, the antibody reacted with globules of variable density in accessory cells. Intense immunolabelling was observed in the vacuole-like structures of the globules. Sometimes, products of the specific immunocytochemical reaction were found in the Golgi apparatus in these globules. Quantitative examination indicated that FSG was actively produced by the accessory cells from the late non-breeding season to the pre-breeding season. These results suggest that there are marked seasonal variations in the production of FSG by the accessory cells in the sea urchin ovary. These findings also provide new evidence that accessory cells exhibit dynamic changes during the reproductive process in the sea urchin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00188689
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 27
    Electronic Resource
    Electronic Resource
    Cambium: old challenges – new opportunities (1999)
    Springer
    Trees 13 (1999), S. 138-151 
    Details
    ISSN: 0931-1890
    Keywords: Key words Cytoskeleton ; Immunocytochemistry ; Model systems ; Populus ; Secondary vascular system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Trees represent a, probably the, major component of the biosphere and have a unique place in the history of Mankind. One of their most fascinating features is the process of secondary growth which is effected principally by the secondary vascular system, the developmental continuum of secondary phloem, vascular cambium, and secondary xylem. However, for too long assumptions about the developmental biology of trees have had to be based upon studies of primary growth systems within annual, herbaceous species because study of the secondary vascular system had been largely ignored. Even when attempts are made to understand some of the most fundamental features of the secondary vascular system, such as xylogenesis, the current model system, isolated Zinnia mesophyll cells, is not entirely appropriate to the situation in the intact tree. Some deficiencies of the Zinnia system are discussed, and the advantages of the genus Populus as a model for study of the hardwood secondary vascular system are considered. Some of the new approaches which are poised to lead to significant advances in our knowledge of the cell bio-logy of the secondary vascular system of trees – spe-cifically of the cell wall, the plasmalemma, and the cytoskeleton – are discussed. The value of one of these new techniques – immunocytochemistry – is demonstrated by a consideration of recent work on the role of the cytoskeleton in the hardwood secondary vascular system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00009745
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 28
    Electronic Resource
    Electronic Resource
    Demonstration of enamel matrix proteins on root-analogue surfaces of rabbit permanent incisor teeth (1977)
    Springer
    Calcified tissue international 24 (1977), S. 223-229 
    Details
    ISSN: 1432-0827
    Keywords: Enamel-cementum-morphology ; Immunocytochemistry ; Biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The continuously erupting rabbit incisor tooth is normally thought of as having an enamel covered “crown” on its labial surface and a cementum covered “root” on its lingual surface. We have examined both surfaces of continuously erupting rabbit incisor teeth taken from near term embryos by a variety of means, including transmission and scanning electron microscopy, biochemical fractionation, and immunohistochemistry. In all cases, we could detect no qualitative difference in the early extracellular matrices taken from the labial and lingual surfaces of the teeth. Both matrices were shown to be composed of dentin and enamel, although the thickness and geometry of the enamel matrix on the lingual surface was somewhat different from that on the labial surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02223320
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 29
    Electronic Resource
    Electronic Resource
    Extracellular protein fibrils in Chrysochromulina breviturrita (Prymnesiophyceae) and their serological relationship to fungal fimbriae (1986)
    Springer
    Archives of microbiology 146 (1986), S. 207-213 
    Details
    ISSN: 1432-072X
    Keywords: Extracellular proteins ; Surface fibrils ; Algae-fungi-Chrysochromulina ; Immunocytochemistry ; Agglutination ; Fimoriae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An extensive network of extracellular fibrils was revealed by negative staining in the greenish gold algal flagellate, Chrysochromulina breviturrita. These fibrils were of uniform diameter (4–5 nm), sometimes exceeding 5 μm in length. In addition there were short, narrower fibrils (2–3 nm) on the surface of the flagella. Six protein bands were isolated from spent culture medium by SDS-PAGE and one of 80,000 Da was found to polymerize after dialysis into 4–5 nm fibrils identical to those found on the cell surface. Two other proteins of 58,000 Da and 65,000 Da also formed 4–5 nm fibrils but these were either rare or of a shorter length and different appearance. An antiserum directed against the surface 7 nm fibrils (fimbriae) of fungi agglutinated cells of C. breviturrita and some other Prymnesiophyceae and Chrysophyceae, but did not agglutinate cells of algal species in other groups. Immunofluorescence and protein A gold labelling confirmed that antigens related to fungal fimbriae were present on the surface of cells of C. breviturrita. Only the 80,000 and 58,000 Da proteins labelled heavily following protein A gold labelling. Some individual 4–5 nm fibrils labelled with gold were observed in the material prepared from the 80,000 Da band. These results therefore establish that C. breviturrita produces a surface network of fibrils that are serologically related to the fimbriae of fungi, and suggest a previously unrecognized relationship between members of the Prymnesiophyceae, Chrysophyceae and fungal groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403218
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 30
    Electronic Resource
    Electronic Resource
    Localization of nitrogenase in vesicles of Frankia sp. Cc1.17 by immunogoldlabelling on ultrathin cryosections (1987)
    Springer
    Archives of microbiology 146 (1987), S. 327-331 
    Details
    ISSN: 1432-072X
    Keywords: Actinomycetes ; Nitrogen fixation ; Symbiosis ; Immunocytochemistry ; Ultracryotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunogoldlabelling on ultrathin cryosections of Frankia sp. Cc1.17 showed specific labelling of nitrogenase in the spherical cells called vesicles. No label was found in the hyphae in any cells grown on a medium with combined nitrogen, nor in those to which no specific antiserum was added. Similar results were obtained with cultures grown under high (20%) and low (2%) oxygen tension in the gas phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410930
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 31
    Electronic Resource
    Electronic Resource
    Dihydroxyacetone synthase is localized in the peroxisomal matrix of methanol-grown Hansenula polymorpha (1985)
    Springer
    Archives of microbiology 143 (1985), S. 237-243 
    Details
    ISSN: 1432-072X
    Keywords: Hansenula polymorpha ; Peroxisomes ; Methanol ; Dihydroxyacetone synthase ; Cell fractionation ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The subcellular localization of dihydroxyacetone synthase (DHAS) in the methylotrophic yeast Hansenula polymorpha was studied by various biochemical and immunocytochemical methods. After cell fractionation involving differential and sucrose gradient centrifugation of protoplast homogenates prepared from methanol-grown cells, DHAS cosedimented with the peroxisomal enzymes alcohol oxidase and catalase. Electron microscopy of this fraction showed that it contained mainly intact peroxisomes, whereas SDS-polyacrylamide gel electrophoresis revealed two major protein bands (75 and 78 kDa) which were identified as alcohol oxidase and DHAS, respectively. The localization of DHAS in peroxisomes was further established by immunocytochemistry. After immuno-gold staining carried out on ultrathin sections of methanol-grown H. polymorpha using DHAS-specific antibodies, labelling was confined to the peroxisomal matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411242
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 32
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum (1987)
    Springer
    Archives of microbiology 147 (1987), S. 190-194 
    Details
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Methyl-CoM reductase ; Immunocytochemistry ; Colloidal gold ; Energy conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of Methanobacterium thermoautotrophicum were fixed with glutaraldehyde, sectioned and labeled with antibodies against the β subunit of component C (=methyl-CoM reductase) of methyl-CoM reductase system and with colloidal gold-labeled protein A. It was found that the gold particles were located predominantly in the vicinity of the cytoplasmic membrane, when the cells were grown under conditions where methyl-CoM reductase was not overproduced. This finding confirms the recent data obtained with Methanococcus voltae showing via the same immunocytochemical localization technique that in this organism methyl-CoM reductase is membrane associated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00415283
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 33
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of two key enzymes of the 2-hydroxyglutarate pathway of glutamate fermentation in Acidaminococcus fermentans (1988)
    Springer
    Archives of microbiology 150 (1988), S. 504-508 
    Details
    ISSN: 1432-072X
    Keywords: Acidaminococcus fermentans ; Glutamate fermentation ; Electron microscopy ; Immunocytochemistry ; Post-embedding labelling ; Antibody-gold complexes ; Protein A-gold complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the in situ location of glutaconyl-CoA decarboxylase and 2-htdroxyglutaryl-CoA dehydratase in Acidaminococcus fermentans using the antibody-gold and protein A-gold techniques carried out as a post-embedding immunoelectron microscopic procedure. Polyclonal antisera were raised in rabbits against homogeneous fractions of the enzymes. Anaerobically grown cells of A. fermentans of the late exponential growth phase were fixed with 0.2% glutaraldehyde and 0.3% formaldehyde (final concentrations) in the growth medium. Dehydration of the cells was achieved with methanol. The cells were embedded in the low temperature embedding resin Lowicryl K4M. The markers indicative for antigenic sites of the two enzymes unequivocally demonstrate that the sodium pump glutaconyl-CoA decarboxylase is located at the cell periphery being a membrane-bound enzyme as expected whereas 2-hydroxyglutaryl-CoA dehydratase is a soluble cytoplasmic enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422295
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 34
    Electronic Resource
    Electronic Resource
    Immunogold silver staining for light microscopy (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 9-17 
    Details
    ISSN: 1432-119X
    Keywords: Key words Silver enhancement ; Immunogold-silver staining ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The immunogold silver staining method (IGSS) is widely used as a sensitive and specific immunohistochemical visualisation technique. IGSS involves the specific deposition of metallic silver at the site of immunogold labelling and provides a means of visualisation at low magnification by light or electron microscopy. Silver developers for IGSS rapidly deposit metallic silver only at the site of heavy metals, including gold and silver, because of their catalytic activity. The developing solution contains the silver ions and reducing agent necessary for this reaction. Using different silver salts as ion donors and by selecting an appropriate temperature and pH, visible amounts of silver can be deposited in a few minutes at the site of colloidal gold labelling while little non-specific background deposition occurs. Inclusion of protective colloids in the solution can also be used to control the reaction. Although studies of the chemical basis of silver deposition around unlabelled colloidal gold date back to 1939, immunogold enhancement by silver was established in 1983. The IGSS method evolved from the combination of disparate photographic, histochemical and immunogold techniques which have been effectively combined and optimised over the last 10 years to provide a visualisation system which is well suited to many immunohistochemical studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050019
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 35
    Electronic Resource
    Electronic Resource
    Immunogold silver staining for light microscopy (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 9-17 
    Details
    ISSN: 1432-119X
    Keywords: Silver enhancement ; Immunogold-silver staining ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The immunogold silver staining method (IGSS) is widely used as a sensitive and specific immunohistochemical visualisation technique. IGSS involves the specific deposition of metallic silver at the site of immunogold labelling and provides a means of visualisation at low magnification by light or electron microscopy. Silver developers for IGSS rapidly deposit metallic silver only at the site of heavy metals, including gold and silver, because of their catalytic activity. The developing solution contains the silver ions and reducing agent necessary for this reaction. Using different silver salts as ion donors and by selecting an appropriate temperature and pH, visible amounts of silver can be deposited in a few minutes at the site of colloidal gold labelling while little non-specific background deposition occurs. Inclusion of protective colloids in the solution can also be used to control the reaction. Although studies of the chemical basis of silver deposition around unlabelled colloidal gold date back to 1939, immunogold enhancement by silver was established in 1983. The IGSS method evolved from the combination of disparate photographic, histochemical and immunogold techniques which have been effectively combined and optimised over the last 10 years to provide a visualisation system which is well suited to many immunohistochemical studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02473198
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 36
    Electronic Resource
    Electronic Resource
    Circadian photoreception in the retinally degenerate mouse (rd/rd) (1991)
    Springer
    Journal of comparative physiology 169 (1991), S. 39-50 
    Details
    ISSN: 1432-1351
    Keywords: Photoreception ; Retinally degenerate ; Mouse ; Circadian ; Rods ; Cones ; 11-cis retinaldehyde ; Immunocytochemistry ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We have examined the effects of light on circadian locomotor rhythms in retinally degenerate mice (C57BL/6J mice homozygous for the rd allele: rd/rd). The sensitivity of circadian photoreception in these mice was determined by varying the irradiance of a 15 min light pulse (515 nm) given at circadian time 16 and meauring the magnitude of the phase shift of the locomotor rhythm. Experiments were performed on animals 80 days of age. Despite the loss of visual photoreceptors in the rd/rd retina, animals showed circadian responses to light that were indistinguishable from mice with normal retinas (rd/+ and +/+). While no photoreceptor outersegments were identified in the retina of rd/rd animals (80–100 days of age), we did identify a small number of perikarya that were immunoreactive for cone opsins, and even fewer cells that contained rod opsin. Using HPLC, we demonstrated the presence and photoisomerization of the rhodopsin chromophore 11-cis retinaldehyde. The rd/rd retinas contained about 2% of 11-cis retinaldehyde found in +/+ retinas. We have yet to determine whether the opsin immunoreactive perikarya or some other unidentified cell type mediate circadian light detection in the rd/rd retina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198171
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 37
    Electronic Resource
    Electronic Resource
    Periplasmic location of nitrous oxide reductase and its apoform in denitrifying Pseudomonas stutzeri (1992)
    Springer
    Archives of microbiology 157 (1992), S. 218-222 
    Details
    ISSN: 1432-072X
    Keywords: Denitrification ; N2O reductase ; Nitrite reductase ; Immunocytochemistry ; Localization ; Two-dimensional electrophoresis ; Cell fractionation ; Pseudomonas stutzeri
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunogold labelling techniques on ultrathin sections of low temperature embedded cells yielded evidence for the periplasmic location of the respiratory enzymes N2O reductase and nitrite reductase (cytochrome cd 1) in Pseudomonas stutzeri strain ZoBell. Cell fractionation by spheroplast preparation and two-dimensional electrophoresis showed the absence of a membrane association of these enzymes. Immunocytochemical localization of N2O reductase in a mutant strain deficient in the chromophore of N2O reductase showed the gold label at the cell periphery, indicating that the copper chromophore processing takes place after export of this protein's apoform.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00245153
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 38
    Electronic Resource
    Electronic Resource
    Chromosomal location by F1 monosomic analysis of endosperm proteins in bread wheat (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 933-940 
    Details
    ISSN: 1432-2242
    Keywords: Gliadin ; Two-dimensional electrophoresis ; F1 monosomic analysis ; chromosomal assignment ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gliadin components from four bread wheat cultivars: Chinese Spring, Capelle Desprez, Holdfast and Pane-247 and their monosomic F1s for the chromosomes of homoeologous groups 1 and 6 have been analyzed by two-dimensional (2-pH) polyacrylamide gel electrophoresis. Chromosomal location of gliadin genes and the allelic differences were well established by analyzing the different F1 monosomic hybrids, electrophoretical patterns and differences in relative staining intensity. A new gliadin encoded by a gene located on chromosome 6B in Chinese Spring is described. The two-dimensional patterns of gliadin in the other three varieties and the chromosomal location of their genes are reported for the first time. Relationships between gliadins in the two-dimensional patterns and the traditional system for their nomenclature are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273684
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 39
    Electronic Resource
    Electronic Resource
    Localization of phytohemagglutinin in the embryonic axis of Phaseolus vulgaris with ultra-thin cryosections embedded in plastic after indirect immunolabeling (1984)
    Springer
    Planta 162 (1984), S. 548-555 
    Details
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; Lectin (localization) ; Phaseolus (lectin) ; Phytohemagglutinin ; Seed (lectin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined the properties and subcellular localization of phytohemagglutinin (PHA), the major lectin of the common bean (Phaseolus vulgaris.), in the axis cells of nearly mature and imbibed mature seeds. On a protein basis the axis contained about 15% as much PHA as the cotyledons. Localization of PHA was done with an indirect immunolabeling method (rabbit antibodies against PHA, followed by colloidal gold particles coated with goat antibodies against rabbit immunoglobulins) on ultra-thin cryosections which were embedded in plastic on the grids after the immunolabeling procedure. The embedding greatly improved the visualization of the subcellular structures. The small (4 nm) collodial gold particles, localized with the electron microscope, were found exclusively over small vacuoles or protein bodies in all the cell types examined (cortical parenchyma cells, vascular-bundle cells, epidermal cells). The matrix of these vacuoles-protein bodies appears considerably less dense than that of the protein bodies in the cotyledons, but the results confirm that in all parts of the embryo PHA is localized in similar structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00399921
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 40
    Electronic Resource
    Electronic Resource
    Phytochrome photoreversibility: Empirical test of the hypothesis that it varies as a consequence of pigment compartmentation (1978)
    Springer
    Planta 141 (1978), S. 129-134 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Immunocytochemistry ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome of oat (Avena sativa L., cv. Garry) coleoptile cells in the red-light-absorbing form, Pr, is diffusely distributed while after conversion to the far-red-light-absorbing form, Pfr, it is observed only in very small areas within the cell. Comparison of phytochrome photoversibility measurements to the distribution of the pigment within the cell indicates that the spectral assay is not influenced by the observed compartmentalization of the chromoprotein. However, the observed compartmentalization of phytochrome is correlated with a loss in spectrophotometrically detectable Pr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387878
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 41
    Electronic Resource
    Electronic Resource
    Effect of experience on in-flight orientation to host-associated cues in the generalist parasitoidLysiphlebus testaceipes (1993)
    Springer
    Entomologia experimentalis et applicata 68 (1993), S. 219-229 
    Details
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Aphidiidae ; Homoptera ; Aphididae ; Schizaphis graminum ; wheat ; tritrophic interactions ; learning ; host-habitat location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of experience on the responsiveness of the aphidiid parasitoidLysiphlebus testaceipes (Cresson) (Hymenoptera: Aphidiidae) to host-associated cues was investigated using a wind-tunnel bioassay. Naive females were able to discriminate between uninfested wheat (Triticum aestivum L.) and wheat infested withSchizaphis gramimum (Rondani) (Homoptera: Aphididae), but oviposition experience significantly increased the parasitoid's propensity to respond to aphid-infested plants with upwind, targeted flight. The behavioural change associated with such experience was acquired rapidly (within five minutes) and persisted for at least 24 h. The parasitoid could be successfully conditioned to associate a novel odour with the presence of hosts, suggesting that the increase in response to aphid-infested plants which occurs as a result of experience is probably due to associative learning of olfactory cues from the plant-aphid complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02380774
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 42
    Electronic Resource
    Electronic Resource
    Temporal changes in the egglaying behaviour of the Hessian fly (1989)
    Springer
    Entomologia experimentalis et applicata 53 (1989), S. 17-29 
    Details
    ISSN: 1570-7458
    Keywords: Mayetiola destructor ; Cecidomyiidae ; oviposition ; host specificity ; wheat ; oats ; insect-plant interactions ; thresholds ; deprivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les réactions de femelles préalablement accouplées de Mouches de Hesse ont été examinées en analysant le comportement de femelles isolées sur blé et sur avoine. Le répertoire comportemental de ces femelles comprenait: le vol, l'atterrissage sur feuille, la flexion du corps de sorte que l'extrémité de l'abdomen touchât la surface de la feuille, l'antennation, les mouvements de l'extrémité de l'abdomen sur la feuille à angle droit des nervures, le repos avec la tarière droite et encore dévaginée, le repos avec la tarière télescopée à l'intérieur du corps. Sur blé plus que sur avoine, les femelles après atterrissage ont présenté une fréquence plus élevée de passage de la flexion à l'antennation que de la flexion à l'abdomen droit. Durant les 5 premières minutes d'observation, les individus libérés dans des enceintes avec blé fléchirent et ‘antennèrent’ 2 à 3 fois plus que ceux libérés sur avoine. Les durées des différentes séquences différaient aussi: sur blé, pendant les 5 premières minutes, les femelles passèrent plus de 50% du temps à fléchir, tandis que sur avoine elles passèrent plus de 50% du temps en repos. Les femelles restèrent aussi plus longtemps sur les feuilles de blé et y pondirent 4 fois plus d'oeufs que sur avoine. Les femelles de M. destructor ont montré une plasticité du seuil d'acceptation. Pendant les premières heures de ponte, elles ont été très sélectives et refusèrent, ou ne pondirent que quelques oeufs sur avoine, mais acceptèrent volontiers le blé. La discrimination s'est poursuivie tant que les femelles ont eu accès au blé en même temps qu'à l'avoine. Cependant, quand les femelles ont été privées de blé pendant plusieures heures, l'acceptation de l'avoine a augmenté. Cet accroissement de l'acceptation a eu lieu à peu près au moment où les femelles sur blé pondaient leurs derniers oeufs.
    Notes: Abstract Responses of mated female Hessian flies were investigated by analysing the behaviour of individual flies in wheat and oats. The behavioural repertoire of such females included: flying, alighting on leaves, arching of the body so that the tip of the abdomen touched the leaf surface, antennation, movements of the tip of the abdomen across the leaf at right angles to leaf veins, sitting with the ovipositor straight but still extended, and sitting with the ovipositor telescoped into the body. After alighting, females on wheat showed a higher frequency of transitions from arching to antennation and a lower frequency of transitions from arching to abdomen straight than females on oats. During the first 5 min of observations, individuals released into arenas with wheat arched and antennated 2–3 times more frequently than females released into oats. Time allotted to behaviours also differed; during the first 5 min, females in wheat spent 50 percent more time arching, whereas females in oats spent 50 percent more time sitting. Females in wheat also stayed longer and laid 4 times more eggs than females in oats. Temporal changes in egglaying were monitored by quantifying hourly rates of egglaying in no-choice assays for several hours following mating at 9:00 am. During the first and second hours post-mating, egglaying occurred infrequently. However, during the third hour post-mating (11:00 am to noon) females on wheat laid 5 times more eggs than females on oats. Rates of egglaying decreased on wheat but increased on oats during the fourth hour, and then during the fifth hour, decreased on both wheat and oats. Changes in egglaying responses were also evident when behaviours of individual females were measured 1–3 h vs. 3–7 h post-mating. Females deprived of host plants and released into wheat or oats later in the day showed higher frequencies of arching and antennation and laid more eggs before leaving the arena.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369228
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 43
    Electronic Resource
    Electronic Resource
    Comparison of Diuraphis noxia resistance in wheat isolines and plant introduction lines (1999)
    Springer
    Entomologia experimentalis et applicata 92 (1999), S. 157-164 
    Details
    ISSN: 1570-7458
    Keywords: plant resistance ; antibiosis ; tolerance ; antixenosis ; Russian wheat aphid ; wheat ; Homoptera ; Aphididae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Russian wheat aphid, Diuraphis noxia (Mordvilko), is one of the most important aphid pests of wheat, Triticum aestivum L., worldwide. Among the various pest management options, plant resistance is an economical management tactic to control D. noxia in cereal crops such as wheat. Researchers have identified D. noxia resistant germplasm and it has been incorporated into wheat. This study compared D. noxia resistance between the ‘Betta’ wheat isolines Betta-Dn1, Betta-Dn2, and Betta-Dn5 and their corresponding donor gene plant introduction (PI) lines PI 137739 (Dn1), PI 262660 (Dn2), and PI 294994 (Dn5). Although the Betta isolines and PI lines showed D. noxia resistance when compared with Betta wheat, the degree of resistance in the isolines to D. noxia was different from their corresponding PI donors. Aphid number, aphid fecundity, and biomass per aphid were not different between Betta-Dn1 and PI 137739 or Betta-Dn2 and PI 262660; however, the same parameters were significantly lower on PI 294994 compared with Betta-Dn5. This indicated that aphid resistance in PI 137739 and PI 262660 was probably governed by a single dominant gene, while the resistance in PI 294994 was controlled by more than one gene. Additionally, plant biomass reduction was aphid density dependent, which suggested that use of appropriate aphid infestation level is important when using plant biomass reduction as an indicator of resistance. Plant resistance categorization showed that there was no detectable difference in antixenosis among the seven lines evaluated. However, the higher aphid fecundity observed on PI 262660 compared with PI 137739 and PI 294994, in addition to no significant differences among the three PIs in plant biomass reduction, suggested PI 262660 was a tolerant line, while PI 137739 and PI 294994 were antibiotic lines. Plant tolerance could not be elucidated among the three Betta isolines using aphid fecundity and plant biomass reduction as indicators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003719325094
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 44
    Electronic Resource
    Electronic Resource
    Within-plant distribution of Rhopalosiphum padi on wheat seedlings is affected by induced responses (1999)
    Springer
    Entomologia experimentalis et applicata 93 (1999), S. 227-230 
    Details
    ISSN: 1570-7458
    Keywords: Rhopalosiphum padi ; cereal aphids ; wheat ; induced responses ; feeding site
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003874225681
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 45
    Electronic Resource
    Electronic Resource
    Hypersensitive response of wheat to the Hessian fly (1995)
    Springer
    Entomologia experimentalis et applicata 74 (1995), S. 283-294 
    Details
    ISSN: 1570-7458
    Keywords: hypersensitivity ; Hessian fly ; plant resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hessian flyMayetiola destructor (Say) larvae are able to obtain food from their host plant without inflicting mechanical damage to the plant surface, apparently by secreting substances which elicit release of nutrients from plant cells surrounding the feeding site. Cells of fully susceptible plants retain their normal appearances, while in resistant plants extensive areas of cellular collapse occur. These responses indicate that hypersensitivity is the basis of wheat's resistance to the Hessian fly. The fly's feeding mechanism more closely resembles that of a pathogen than of a phytophagous insect; correspondingly, both the genetic relationship and resistance mechanism of the host plant to the parasite are of the sorts commonly associated with bacterial and fungal pathogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02381793
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 46
    Electronic Resource
    Electronic Resource
    La répartition spatiale des différents stades de Coccinella septempunctata dans un champ de blé: variation au cours de la saison (1989)
    Springer
    Entomologia experimentalis et applicata 53 (1989), S. 229-236 
    Details
    ISSN: 1570-7458
    Keywords: Coccinella septempunctata ; Coccinellidae ; wheat ; egg laying ; larva ; adult ; vertical distribution ; Coccinella septempunctata ; Coccinellidae ; blé ; ponte ; larve ; adulte ; répartition spatiale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary At the beginning of spring, post-hibernating adults of Coccinella septempunctata L. (Col., Coccinellidae), larvae and nymphs are found on the lower part of wheat plants. In June, adults born in the field as well as their progeny are dispersed all over the plant and a significant proportion of them is found on the apical parts of plants. Most of the egg clutches are layed on soil lumps, stones and small wild plants which represent preferential sites. Variations in coccinellid distribution may be influenced by climatic conditions, especially the temperature, by the thermophilic nature of this species and by the repartition of the three main aphid species on the plants. These results are relevant for the selection of a sampling methods.
    Notes: Résumé Au début du printemps les adultes de Coccinella septempunctata L. (Col., Coccinellidae) issus des sites d'hivernation, les larves et les nymphes qui en proviennent, fréquentent le sol et les parties basses du blé. En juin, les adultes nés dans le champ et leur descendance sont dispersés sur toute la hauteur des talles bien qu'une fraction non négligeable de leur population se rencontre à l'extrémité supérieure. La plupart des pontes sont déposées sous les mottes de terre, les pierres et les petites adventices qui constituent des sites privilégiés. Ces variations de la distribution de cette coccinelle peuvent être mises en relation avec le climat, en particulier la température, avec la thermophilie de cette espèce et avec la répartition sur les différentes parties des talles des 3 espèces de puceron qui se succèdent au cours de la saison. Ces observations sont importantes à considérer lors du choix ou de la mise au point d'une méthode d'échantillonnage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00162854
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 47
    Electronic Resource
    Electronic Resource
    Influence of cereal leaf epicuticular wax on Diuraphis noxia probing behavior and nymphoposition (1998)
    Springer
    Entomologia experimentalis et applicata 89 (1998), S. 111-118 
    Details
    ISSN: 1570-7458
    Keywords: leaf surface wax ; probing behavior ; nymphoposition ; Russian wheat aphid ; wheat ; barley ; oat ; Homoptera ; Aphididae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of cereal leaf surface wax on Diuraphis noxia (Mordvilko), the Russian wheat aphid, probing behavior and nymphoposition was evaluated. Ultrastructure of leaf epicuticular wax from wheat (Triticum aestivum L.) c.v. ‘Arapahoe’ and ‘Halt’ was different from barley (Hordeum vulgare L.) c.v. ‘Morex’, and oat (Avena sativa L.) c.v. ‘Border’. Both wheat cultivars had similar rod-shaped epicuticular wax, while barley and oat plants had flakes. The chemical composition comparison of gas chromatograms also indicated that the extract of the two wheat cultivars had similar pattern of peaks, while the barley and oat leaves had similar peaks. Cereal variety significantly affected aphid probing behavior (P 〈 0.05), but wax removal using ethyl ether swab did not (P 〈 0.05). Aphids initiated significantly more probes on Border oat leaves than on Morex barley irrespective of wax removal, although total probing duration per aphid was not significantly different among the four cereals examined. Accumulative salivation duration per aphid on oat leaves with wax was significantly longer than other cereal leaves with wax, while accumulative ingestion duration per aphid on Arapahoe wheat and Morex barley was significantly longer than on oat. Nymphoposition of D. noxia on cereal leaves maintained on the benzimidazole-agar medium showed that aphids produced a greater number of nymphs on Morex barley and less on Border oat leaves, although wax removal did not affect aphid nymphoposition. Removal of leaf epicuticular waxes from the 4 cereal genotypes using ethyl ether swab indicated that the influence of wax on plant resistance to D. noxia probing and reproduction was limited. Morex barley was the most favorable, while Border oat was the least favorable cereal host of D. noxia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003458406502
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 48
    Electronic Resource
    Electronic Resource
    Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells (1996)
    Springer
    Journal of plant growth regulation 15 (1996), S. 95-102 
    Details
    ISSN: 1435-8107
    Keywords: Cell cycle ; Cytokinin ; Soybean cells ; Glycine max ; Immunocytochemistry ; S phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An immunocytochemical method was used to determine the proportion of cells in the DNA synthesis (S phase) of the mitotic cycle in suspension cultures of soybean (Glycine max (L.) Merr. cv. Acme) callus of cotyledonary origin, the stably cytokinin-dependent tissue used in the cytokinin bioassay devised by Carlos O. Miller. A standard cell synchronization protocol involving hydroxyurea was used to demonstrate the applicability of the immunocytochemical method to this cell culture. Cells were brought to mitotic arrest by cytokinin withdrawal, and the cell division cycle was restarted by the addition of cytokinin. We have followed the pattern of resumption of S phase after the readdition of cytokinin. This pattern reveals the existence of three subpopulations of cells in cytokinin-starved cultures, consistent with the occurrence of three cytokinin-requiring events in the cell cycle: one in mitosis, one in S phase, and one in the G1 phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192938
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 49
    Electronic Resource
    Electronic Resource
    Dietary modulation of α-amylase activity in eight geographical strains of Sitophilus oryzae and Sitophilus zeamais (1988)
    Springer
    Entomologia experimentalis et applicata 46 (1988), S. 47-54 
    Details
    ISSN: 1570-7458
    Keywords: Coleoptera ; Curculionidae ; Sitophilus ; S. oryzae ; S. zeamais ; rice weevil ; maize weevil ; geographical strains ; digestion ; amylase ; diet ; barley ; corn ; maize ; rice ; wheat ; amylase inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé La transformation de rang des valeur d'activité spécifique de l'α-amylase de 4 souches de S. oryzae et de 4 souches de S. zeamais montre que les niveaux les plus élevés de ces enzymes prédominantes s'observent chez les adultes nourris d'orge mondé ou de riz brun á grains longs. Des niveaux intermédiaires d'activité ont été obtenus chez les insectes élevés sur maïs jaune, et les niveaux les plus faibles chez ceux élevés sur blé. Bien que les extraits préparés à partir d'orge présentent une activité inhibitrice de deux isoamylases purifiées de S. oryzae, les niveaux des inhibiteurs naturels α-amylase de ces deux enzymes sont environ respectivement 2,2 et 6,1 fois plus concentrés dans le blé. L'ingestion de ces inhibiteurs d'amylase et la formation d'un complexe enzyme inactive/inhibiteur avec l'amylase secrétée antérieurement, peut rendre compte de la plus faible activité de l'amylase chez les charançons consommant du blé. Le niveau d'amylase de S. oryzae est 2 fois plus élevé que celui de S. zeamais pour toutes les souches élevées sur un régime donné. Des niveaux d'activité significativement différents ont été trouvés suivant les souches pour chacune des deux espèces. Puisque l'amylase est la principale hydrolase digestive de ces espèces, l'intensité de la modification des teneurs en amylase par la consommation de céréales peut indiquer leur adéquation comme hôtes potentiels.
    Notes: Abstract Rank transformation of specific activity values of α-amylase across four strains of Sitophilus oryzae (L.) and four strains of S. zeamais Motschulsky indicates that levels of these predominant enzymes are highest in adults feeding on hulled barley or long-grain brown rice. Intermediate activity levels are found in weevils feeding on yellow corn (maize) and lowest levels are found in wheat-fed weevils. Although extracts prepared from barley contain inhibitory activity against two purified isoamylases from S. oryzae, levels of the naturally-occurring α-amylase inhibitors against these two enzymes are about 2.2-fold and 6.1-fold, respectively, more concentrated in wheat. Ingestion of these amylase inhibitors and formation of an inactive enzyme:inhibitor complex with previously secreted amylase may account for the lower activity of amylase in weevils of both species feeding on wheat. Amylase levels across all strains feeding on a given diet are about 2-fold higher in S. oryzae than in S. zeamais. Significant differences in activity levels were also found between strains in both species. Since α-amylase is a predominant digestive hydrolase in these species, the degree to which cereal diets affect amylase levels may indicate their suitability as potential hosts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00186999
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 50
    Electronic Resource
    Electronic Resource
    Influence of within-field and landscape factors on aphid predator populations in wheat (1999)
    Springer
    Landscape ecology 14 (1999), S. 239-252 
    Details
    ISSN: 1572-9761
    Keywords: Coccinellidae ; Aphididae ; wheat ; spatial scale ; species diversity ; numerical response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of prey density, within-field vegetation, and the composition and patchiness of the surrounding landscape on the abundance of insect predators of cereal aphids was studied in wheat fields in eastern South Dakota, USA. Cereal aphids, aphid predators, and within-field vegetation were sampled in 104 fields over a three year period (1988–1990). The composition and patchiness of the landscape surrounding each field were determined from high altitude aerial photographs. Five landscape variables, aggregated at three spatial scales ranging from 2.6 km2 to 581 km2, were measured from aerial photographs. Regression models incorporating within-field and landscape variables accounted for 27–49% of the variance in aphid predator abundance in wheat fields. Aphid predator species richness and species diversity were also related to within-field and landscape variables. Some predators were strongly influenced by variability in the composition and patchiness of the landscape surrounding a field at a particular spatial scale while others responded to variability at all scales. Overall, predator abundance, species richness, and species diversity increased with increasing vegetational diversity in wheat fields and with increasing amounts of non-cultivated lands and increasing patchiness in the surrounding landscape.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008002528345
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 51
    Electronic Resource
    Electronic Resource
    A Modular Vector for Agrobacterium Mediated Transformation of Wheat (1999)
    Springer
    Plant molecular biology reporter 17 (1999), S. 323-331 
    Details
    ISSN: 1572-9818
    Keywords: Agrobacterium ; modular vector ; transformation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat (cv Chinese Spring) tissues were transformed using Agrobacterium tumefasciens and a new plasmid modular vector, pMVTBP. We constructed pMVTBP with unique restriction sites connecting (1) the CaMV 35S promoter, (2) a Kozak sequence, (3) the FLAG epitope, (4) the (His)6 epitope, (5) a coding region (for wheat TATA Binding Protein, wTBP) and (6) the CaMV 35S 3′UTR. This vector thus allows easy exchange of different regulatory or coding sequences. Explants of either germinating mature seeds, or immature embryos, were induced to callus for up to two weeks, treated with virulence-induced bacteria for one hour, then regenerated into plantlets. Transient expression of a GUS reporter gene, assayed at about one week, occurred in 10–12% of calluses. Expression of the FLAG-tagged wTBP was also detected, by immunostaining. Stable expression, by selective growth on geneticin, and by GUS expression at about six weeks, occurred in 1–2% of calluses, quite comparable to that achieved by other methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007686408369
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 52
    Electronic Resource
    Electronic Resource
    Management of Stored Wheat Insect Pests in the USA (1999)
    Springer
    Integrated pest management reviews 4 (1999), S. 127-143 
    Details
    ISSN: 1572-9745
    Keywords: wheat ; stored-grain ; integrated pest management ; aeration ; biological control ; grain sampling ; insect monitoring ; modeling ; area-wide IPM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Management of stored-grain insect pests by farmers or elevator managers should be based upon a knowledge of the grain storage environment and the ecology of insect pests. Grain storage facilities and practices, geographical location, government policies, and marketing demands for grain quality are discussed as factors influencing stored-grain insect pest management decisions in the United States. Typical practices include a small number of grain samples designed to provide grain quality information for segregation, blending and marketing. This low sampling rate results in subjective evaluation and inconsistent penalties for insect-related quality factors. Information on the efficacy of insect pest management practices in the United States, mainly for farm-stored wheat, is discussed, and stored-grain integrated pest management (IPM) is compared to field-crop IPM. The transition from traditional stored-grain insect pest control to IPM will require greater emphasis on sampling to estimate insect densities, the development of sound economic thresholds and decision-making strategies, more selective use of pesticides, and greater use of nonchemical methods such as aeration. New developments in insect monitoring, predictive computer models, grain cooling by aeration, biological control, and fumigation are reviewed, their potential for improving insect pest management is discussed, and future research needs are examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009682410810
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 53
    Electronic Resource
    Electronic Resource
    Mycotoxin formation in HY-320 wheat during granary storage at 15 and 19% moisture content (1990)
    Springer
    Mycopathologia 111 (1990), S. 181-189 
    Details
    ISSN: 1573-0832
    Keywords: mycotoxin ; ochratoxin ; Penicillium ; storage ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Eleven-kilogram parcels of HY-320 wheat, a cultivar of the new Canada Prairie Spring class, were kept at 15 and 19% initial moisture contents (IMC) in simulated storage in a Manitoba farm granary for 60 weeks to determine biotic and abiotic changes and mycotoxin production. Ochratoxin A reached a maximum of 0.24 ppm by week 20 in the 19% IMC wheat, but was absent in the 15% IMC wheat; no other mycotoxins were detected. Temperature, moisture content, O2 and CO2 levels, fat acidity values, seed germination, microfloral incidence and abundance, and the presence of other mycotoxins were monitored. Principal component analysis of all variables showed that the first principal components accounted for 32–41% of the system variability, and contained the ochratoxin A variable. Ochratoxin A was produced in moist grain that had decreased seed germination andAltermaria activity, and high fungal activity byPenicillium andAspergillus versicolor. Compared to other stored cereals previously studied, HY-320 wheat would be ranked in a low-risk category for mycotoxin formation, based on the ochratoxin A levels observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02282802
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 54
    Electronic Resource
    Electronic Resource
    Effects of aflatoxin B1 on in vitro cultures ofNicotiana tabacum var. Samsun (1994)
    Springer
    Mycopathologia 125 (1994), S. 107-117 
    Details
    ISSN: 1573-0832
    Keywords: Aflatoxin B1 ; Immunocytochemistry ; Regeneration ; Tissue culture ; Tobacco plantlets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effects of aflatoxin B1, (0.5–25 µg ml−1) on in vitro root and shoot development in young tobacco explants were investigated. Despite an initial apparent stimulatory effect on most measured parameters at 0.5 µg ml−1 AFB1, the number of leaves, root and leaf mass per plantlet were progressively inhibited with increasing AFB1 concentration. The number of explants developing roots was reduced to 34% at the highest (25 µg ml−1) AFB1 concentration, following 3 weeks exposure to the toxin. Leaf chlorophyll content at this toxin concentration was significantly lower than that measured for control plantlets. Thin layer chromatography confirmed the absorption of AFB1 by the plantlets. Using immunocytochemical techniques, AFB1 was immunolocated predominantly in the vacuoles, the nucleus and the cytoplasm (possibly intravesicularly). The results are discussed in terms of this immunolocation within the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01371099
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 55
    Electronic Resource
    Electronic Resource
    First survey on the natural occurrence of Fusarium mycotoxins in Bulgarian wheat (1996)
    Springer
    Mycopathologia 136 (1996), S. 47-52 
    Details
    ISSN: 1573-0832
    Keywords: Fusarium ; mycotoxins ; occurrence ; trichothecenes ; wheat ; zearalenone ; deoxynivalenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Wheat for human consumption (140 samples) was collected after harvest from all regions of Bulgaria. The 1995 crop year was characterized by heavy rainfall in the spring and summer months. The internal mycoflora of wheat samples was dominated by Fusarium spp. and Alternaria spp., and storage fungi were rarely present. The samples were analysed for contamination with Fusarium mycotoxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), T-2 Toxin (T-2), diacetoxyscirpenol (DAS), and zearalenone (ZEA), using enzyme immunoassay methods. DON and ZEA were the predominant toxins, with a contamination frequency of 67% and 69%, respectively. The average levels of these toxins in positive samples were 180 μg/kg (DON) and 17 μg/kg (ZEA), maximum concentrations were 1800 μg kg−1 and 120 μg kg−1, respectively. Acetyl derivatives of DON, namely 3-AcDON and 15-AcDON, were found in 2.1 % and 0.7% of the samples, at at maximum level of about 100 μg kg−1. Only one sample was positive for T-2 (55 μg/kg), DAS was not detected. This is the first report about the natural occurrence of a range of Fusarium mycotoxins in wheat for human consumption in Bulgaria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436660
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 56
    Electronic Resource
    Electronic Resource
    Fungal volatiles associated with moldy grain in ventilated and non-ventilated bin-stored wheat (1988)
    Springer
    Mycopathologia 101 (1988), S. 53-60 
    Details
    ISSN: 1573-0832
    Keywords: odors ; volatiles ; microflora ; fungi ; wheat ; seasonality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The fungal odor compounds 3-methyl-l-butanol, l-octen-3-ol and 3-octanone were monitored in nine experimental bins in Winnipeg, Manitoba containing a hard red spring wheat during the autumn, winter and summer seasons of 1984–85. Quality changes were associated with seed-borne microflora and moisture content in both ventilated and non-ventilated bins containing wheat of 15.6 and 18.2% initial moisture content. All three odor compounds occurred in considerably greater amounts in bulk wheat in non-ventilated than in ventilated bins, particularly in those with wheat having 18.2% moisture content. The presence of these compounds usually coincided with infection of the seeds by the fungi Alternaria alternata (Fr.) Keissler, Aspergillus repens DeBarry, A. versicolor (Vuill.) Tiraboschi, Penicillium crustosum Thom, P. oxalicum Currie and Thom, P. aurantiogriseum Dierckx, and P. citrinum Thom. High production of all three odor compounds in damp wheat stored in non-ventilated bins was associated with heavy fungal infection of the seeds and reduction in seed germinability. High initial moisture content of the harvested grain accelerated the production of all three fungal volatiles in non-ventilated bins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455669
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 57
    Electronic Resource
    Electronic Resource
    Infection and disintegration of vascular tissues of non-suberized roots of spruce byHeterobasidion annosum and use of antibodies for characterizing infection (1995)
    Springer
    Mycopathologia 129 (1995), S. 91-101 
    Details
    ISSN: 1573-0832
    Keywords: ELISA ; Endodermis ; H. annosum ; Immunocytochemistry ; Root rot ; Vascular tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Vascular disintegration mainly of medulla rays of spruce roots is of major significance in root rot disease of spruce caused byH. annosum. Using seedling roots as an experimental model, the possible routes and initial host reactions preceding invasion of vascular tissues was investigated. Transmission electron microscopy showed that penetration through the endodermis was an obvious route but not without host resistance. Using antibodies againstH. annosum hyphal materials, some labelling of vascular tissues remote from sites of fungal colonization suggest the release of fungal secretory products partly active in tissue disintegration. Similarly, intense labelling was also observed in severely colonized host tissues at late stages of infection. Strong labelling recorded at 3 d p.i. mainly on fungal hyphae and scant gold particles on invaded host tissues could imply that induction of host antifungal metabolites may have been a late event. A correlation was found between total antigenic material in root homogenates measured by ELISA, density of tissue labelling by immunocytochemistry and severity of disease symptoms. The importance of this in relation to diagnosis of biotic root rot diseases in the field is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103468
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 58
    Electronic Resource
    Electronic Resource
    Carbon dioxide laser induction of heat shock protein 70 synthesis: Comparison with high temperature treatment (1995)
    Springer
    Lasers in medical science 10 (1995), S. 207-212 
    Details
    ISSN: 1435-604X
    Keywords: Heat shock protein 70 (hsp70) ; Carbon dioxide laser ; Sodium arsenite ; Human diploid fibroblast ; Electrophoresis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract In a previous study, it was demonstrated that heat shock protein 70 (hsp70) was induced by short duration (〈1 s) carbon dioxide (CO2) laser radiation (10.6Μm. To further characterize the stress response after laser irradiation, the time course of synthesis and cellular localization of hsp70 has been followed. As it had been shown that laser irradiation elevated the temperature to about 67±2
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02133333
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 59
    Electronic Resource
    Electronic Resource
    Statistical properties of chlorophyll fluorescence induction parameters (1998)
    Springer
    Photosynthetica 35 (1998), S. 121-127 
    Details
    ISSN: 1573-9058
    Keywords: Gaussian distribution ; leaf age ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Parameters of the fast chlorophyll (Chl) fluorescence induction (the O-J-I-P curve) of plants of winter wheat grown in the field canopy were statistically tested for Gaussian distribution. Five different statistical methods showed that the obtained values did not obey the Gaussian distribution law. The presentation of the parameters with the help of the mean and standard deviation masks the information about statistical properties of the values. Thus, we recommend to present the parameters by means of median, quartiles, and minimum and maximum values rather than by means of the mean and standard deviation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006886202444
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 60
    Electronic Resource
    Electronic Resource
    Specific Degradation of D1 Protein during Exposure of Thylakoid Membranes to High Temperature in the Dark (1999)
    Springer
    Photosynthetica 36 (1999), S. 433-440 
    Details
    ISSN: 1573-9058
    Keywords: antibody ; polyacrylamide gel electrophoresis ; protease ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exposure of thylakoid membranes to high temperature in dark leads to the degradation of D1 protein. Maximum degradation of D1 protein occurred at 45 °C. Using N-terminal specific D1 antibody, a 23 kDa fragment of D1 protein was detected. The degradation of D1 protein could be prevented both by radical scavengers and inhibitors of serine protease and metallo-protease. These results suggest that degradation of D1 protein during exposure of thylakoid membranes to high temperature in dark is catalyzed by protease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007080204112
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 61
    Electronic Resource
    Electronic Resource
    Interactive Effects of Sulphur Dioxide and Mineral Nutrient Supply on Photosynthetic Characteristics and Yield in Four Wheat Cultivars (2000)
    Springer
    Photosynthetica 38 (2000), S. 91-96 
    Details
    ISSN: 1573-9058
    Keywords: biomass ; carotenoids ; chlorophyll ; nutrients ; sulphur dioxide ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Field experiments were conducted on four cultivars of wheat (Triticum aestivum L.) to examine the variability in cultivar response to sulphur dioxide (SO2) under different concentrations of mineral nutrients. Thirty-days-old plants were exposed for 8 weeks to 390±20 µg m−3 (0.15 ppm) SO2 for 4 h per day, 5 d per week. Decline in net photosynthetic rate, contents of pigments and nitrogen, biomass and grain yield of each cultivars were due to SO2 at all the nutrient concentrations studied. However, the magnitude of reduction was higher in plants grown without nutrient application. On the basis of the reductions in photosynthesis and yield, the susceptibility of wheat cultivars to SO2 was in the order of Malviya 213 〉 Malviya 37 〉 Malviya 206 〉 Malviya 234 at recommended dose of NPK, whereas the same without the nutrients was Malviya 206 〉 Malviya 234 〉 Malviya 213 〉 Malviya 37.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026700109438
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 62
    Electronic Resource
    Electronic Resource
    Formation of cross-linking between photosystem 2 proteins during irradiation of thylakoid membranes at high temperature (1999)
    Springer
    Photosynthetica 36 (1999), S. 213-223 
    Details
    ISSN: 1573-9058
    Keywords: D1 protein ; diphenylcarbazide ; oxygen evolving complex ; scavengers ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Irradiation of thylakoid membranes at 40 °C resulted in complete inhibition of photosystem (PS) 2 activity measured as 2,6-dichlorophenol indophenol (DCIP) photoreduction either in the absence or presence of 1,5-diphenylcarbazide (DPC). Concomitant with the inactivation of PS2 activity, several thylakoid proteins were lost and high molecular mass cross-linking products appeared that cross-reacted with antibodies against proteins of PS2 but not with antibodies against proteins of other three complexes PS1, ATP synthase, and cytochrome b6f. Irradiation of thylakoid membranes suspended in buffer of basic pH or high concentration of Tris at 25 °C resulted in the formation of cross-linking products similar to those in thylakoid membranes irradiated at 40 °C. Presence of radical scavengers and DPC during the high temperature treatment prevented the formation of cross-linking products. These results suggest the involvement of oxygen evolving co mplex (OEC) in the formation of cross-linking between PS2 proteins in thylakoid membrane irradiated at high temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007095426376
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 63
    Electronic Resource
    Electronic Resource
    Relationship between Carbon and Nitrogen Metabolisms in Photosynthesis. The Role of Photooxidation Processes (2000)
    Springer
    Photosynthetica 37 (2000), S. 519-527 
    Details
    ISSN: 1573-9058
    Keywords: alanine ; aspartate ; glycine ; glycollate ; malate ; nitrate ; serine ; sugars ; Triticum aestivum ; urea ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 14CO2 uptake in leaves of wheat plants (Triticum aestivum L.) fertilized by urea or Ca(NO3)2 (25 mol m-3) was investigated. The Warburg effect (inhibition of 14CO2 uptake by oxygen) under 0.03 vol. % CO2 concentration was observed only in non-fertilized plants. Under 0.03 vol. % CO2, the Warburg antieffect (stimulation of 14CO2 uptake by oxygen) was detected only in plants fertilized by Ca(NO3)2. Under saturating CO2 concentration (0.30 vol. %), the Warburg antieffect was observed in all variants. Under limitation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity (0.30 vol. % CO2 + 1 vol. % O2), the rate of synthesis of glycollate metabolism products decreased in control and urea-fertilized plants but was enhanced in nitrate-fed plants. Hence, there was an activation of glycollate formation via transketolase reaction in fertilized plants, and the products of nitrate reduction function were oxidants in nitrate-fertilized plants whereas the superoxide radical played this role in urea-fertilized plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007150921664
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 64
    Electronic Resource
    Electronic Resource
    Polymorphism of prolamins and variability of grain quality (1982)
    Springer
    Plant foods for human nutrition 31 (1982), S. 243-249 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; grain protein ; prolamin ; inheritance ; chromosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Electrophoretic gliadin components are inherited as highly stable blocks of linked components. Multiple allelism of blocks controlled by polygenic loci of six gliadin-coding chromosomes has been revealed. The allelic states of these loci are interrelated with the variability of grain. The regularities discovered permit the use of gliadin polymorphism for estimation of genotype properties in breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01108633
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 65
    Electronic Resource
    Electronic Resource
    Effect of cereal-legumechapati diets on absorption and retention of calcium (1992)
    Springer
    Plant foods for human nutrition 42 (1992), S. 165-173 
    Details
    ISSN: 1573-9104
    Keywords: Calcium ; absorption ; wheat ; Bengal gram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheatchapati, wheat+Bengal gram (80∶20 and 70∶30)chapatis and casein diets, at 10 percent protein level, were fed to rats for 12 weeks to study the effect on calcium utilization. The supplementation of Bengal gram to wheat diets significantly improved the calcium absorption. The urinary calcium excretion in wheat+Bengal gramchapati diets was significantly less than that of wheatchapati diet fed group. The in vivo45Ca absorption (CPM/100 μl serum) was also less in wheatchapati group as compared to wheat+Bengal gramchapati diets fed groups. The supplementation of legumes to cereals improved the calcium utilization and it may be concluded that there will be no risk of occurance of protein induced hypercalciuria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02196469
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 66
    Electronic Resource
    Electronic Resource
    A small scale device for wheat gluten separation (1983)
    Springer
    Plant foods for human nutrition 33 (1983), S. 161-168 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; gluten ; small scale extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new small scale device for wheat gluten separation is described and the results of the first experiments on the influence of extraction conditions on different flours are reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01091303
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 67
    Electronic Resource
    Electronic Resource
    The nutritive value of amaranth grain (Amaranthus caudatus) (1987)
    Springer
    Plant foods for human nutrition 36 (1987), S. 325-334 
    Details
    ISSN: 1573-9104
    Keywords: amaranth grain ; protein and energy utilization ; protein complementation ; maize ; wheat ; sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The purpose of this investigation was to study the nutritional value of grain amaranth (Amaranthus caudatus) as a supplement to cereals, and to determine the effect of heat on nutritional quality of cereal/amaranth mixtures. Maize, wheat and low-tannin sorghum blends containing 0%, 10%, 25% and 50% of popped amaranth flour were studied in balance experiments with growing rats. The popped amaranth was very high in lysine (5.19 g/16g N), but lysine was still the first limiting amino acid in the blends. However, amino acid scores were raised significantly by amaranth substitution, and the tryptophan deficiency of maize protein was alleviated by addition of amaranth. True protein digestibility was, in general, not affected by amaranth substitution, but the biological value increased considerably with increasing levels of inclusion. Amaranth substitution also resulted in remarkable increases in weight gain of the rats. The energy digestibility of the different blends was quite similar and there was only little variation in the amount of digestible energy of the mixtures. Cooking produced a very small but significant reduction in protein and energy digestibility. However, the effects of cooking were minor and probably without practical importance. In conclusion, addition of amaranth to cereal flours improved protein quality without affecting energy utilization. Amaranth seems to be an effective source of protein to combine with cereal proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01892353
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 68
    Electronic Resource
    Electronic Resource
    Influence of competition, irrigation levels and nitrogen fertilization on protein content and protein yield of three spring wheat (Triticum aestivum L.) cultivars (1987)
    Springer
    Plant foods for human nutrition 37 (1987), S. 127-131 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; cultivars ; nitrogen fertilization ; irrigation ; protein ; yield ; content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Protein content and protein yield of three spring wheat cultivars differing in morphological and physiological growth characters were found to be influenced by intercultivar competition, irrigation levels and nitrogen fertilization. The protein content of the tall cultivar C 306 and the protein yield of the dwarf cultivar HD 2160 were more than the other cultivars. Binary mixed stands were not superior to the better component cultivar. Intercultivar competition increased the protein content of dwarf and semi-dwarf cultivars, but decreased the protein content of tall cultivar. On the other hand, protein yield of the dwarf cultivar decreased and that of tall cultivar increased when grown in mixed stands. Protein yield of semi-dwarf cultivar increased when grown with dwarf cultivar, but decreased when grown with tall cultivar. Two or three irrigations increased the protein content and protein yield of all the three cultivars and their mixed stands over one irrigation. Protein content and protein yield of the cultivars and their mixed stands were higher when 150 kg N/ha was applied than when 80 kg N/ha was applied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092048
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 69
    Electronic Resource
    Electronic Resource
    Protein and energy utilization of boiled rice-legume diets and boiled cereals in growing rats (1987)
    Springer
    Plant foods for human nutrition 37 (1987), S. 237-245 
    Details
    ISSN: 1573-9104
    Keywords: protein and energy utilization ; rice ; corn ; sorghum ; wheat ; six rice-legume diets ; phenols ; sugars
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In growing rats, boiled milled rice-legume diets (2:1 N ratio) had lower energy digestigibility than boiled milled rice and equal if not better true digestibility, biological value, and net protein utilization (NPU). Rice-soybean diets showed better NPU than the other rice-legume diets. Boiled whole-grain corn and sorghum had lower digstible energy and NPU than boiled milled rice. NPU of the diets did not follow strictly the trend of the amino acid score of the diets; phenol (tannin) content reduced energy and protein digestibility of all diets, but sugars reduced energy and protein digestibilities of rice-legume diets only.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01091788
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 70
    Electronic Resource
    Electronic Resource
    Improvement of bread wheat gluten quality (1983)
    Springer
    Plant foods for human nutrition 33 (1983), S. 121-126 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; gliadin ; glutenin subunits ; correlation ; gluten quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01091297
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 71
    Electronic Resource
    Electronic Resource
    Nutritive value of protein fractions extracted from soybean, rapeseed and wheat flours in the rat (1984)
    Springer
    Plant foods for human nutrition 34 (1984), S. 243-251 
    Details
    ISSN: 1573-9104
    Keywords: nutritive value ; protein fractions ; rapeseed ; soybean ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The nutritive value of various protein fractions was studied. Fractions 2S and 12S from rapeseed, 2S, 7S and 11S from soybean were obtained by dissolution in ammonium sulfate solutions. Albumin-globulin, gluten, glutenin and gliadin fractions from wheat were obtained by dissolution in salted water (albumin-globulin), acetic acid (glutenin) and alcohol (gliadin). Liveweight gains, protein efficiency ratio (PER) and apparent digestibility coefficient (ADC) were used as measures of the nutritive value. The protein fractions had a lower nutritive value than the unfractionated proteins except for the albumin-globulin fraction of wheat which had a nutritive value higher than that of the unfractionated wheat protein. PER obtained with the rapeseed 2S and 12S fractions were 2.49 and 2.21, respectively, as compared to 2.64 for unfractionated rapeseed. With soybean fractions, PER were 0.92 for 2S, — 0.007 for 7S and 1.47 for 11S, as compared to 2.19 for the original protein. The wheat albumin-globulin fraction gave a PER of 2.78, as compared to 1.45 for the unfractionated wheat protein. Gluten, glutenin and gliadin fractions had a lower PER than that of unfractionated wheat protein. ADC of all fractions were higher than those of the original proteins. The difference in liveweight gains and PER observed between protein fractions can be partially explained on the basis of the essential amino acid content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01126553
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 72
    Electronic Resource
    Electronic Resource
    The use of genetics in understanding protein composition and grain quality in wheat (1982)
    Springer
    Plant foods for human nutrition 31 (1982), S. 205-227 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; grain quality ; endosperm protein ; gliadin ; glutenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract As each of the classes of wheat-grain protein has been implicated in some aspect of man's interest in wheat utilization, we are motivated to learn more about the genetic control of their synthesis so that breeders may better tailor wheats to specific requirements. The gliadins have particularly merited study because they appear to be responsible for coeliac condition and for depressed lysine content, and as they are proving valuable for varietal identification and grain-quality prediction. Genetic aspects of gliadin synthesis have been studied using aneuploids, by examining F1 and F2 segregation after crossing, and by computer comparison of the gliadin composition of many genotypes in conjunction with systematic pedigree comparisons. These studies indicate gliadin synthesis to be controlled by blocks of tightly linked genes on the short arms of the chromosomes of groups 1 and 6. The high molecular weight subunits of glutenin are genetically distinct from the gliadins, being coded by genes on the long arms of chromosomes 1A, 1B and 1D. A better understanding of the relationship between grain quality and specific endosperm proteins is now developing. It is likely to provide simpler means of selecting for quality type in both breeding and harvest segregation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01108631
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 73
    Electronic Resource
    Electronic Resource
    The influence of milling on the nutritive value of flour from cereal grains. 2. Wheat (1983)
    Springer
    Plant foods for human nutrition 33 (1983), S. 51-61 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; milling utilization of protein ; energy ; zinc and other minerals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat was milled into flours having extraction rates between 100 and 66%. The nutritive value of the various fractions was studied by chemical analyses and in balance trials with rats. The concentration of essential nutrients decreased when the extraction rate was lowered. The lysine content (g/16 g N) e.g. was 2.52 in whole wheat, but only 2.18 in the 66% extraction flour; however, only a slight reduction in biological value was found. The content of minerals was reduced to 30% of that in whole wheat, and the apparent zinc absorption and retention expressed in absolute values, were significantly higher from the flours of high extraction than from the more refined flours, in spite of a much higher phytate content in whole wheat and lightly milled flours. It could be concluded that milling of wheat into highly refined flours not only preclude considerable amounts of nutrients from human consumption, but the remaining flours have a much poorer nutritive value than flour made from whole wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01093737
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 74
    Electronic Resource
    Electronic Resource
    The genetics of gliadin and glutenin, the major storage proteins of the wheat endosperm (1982)
    Springer
    Plant foods for human nutrition 31 (1982), S. 229-241 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; genetics ; endosperm ; seed proteins ; gliadins ; glutenins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper reviews our present knowledge of the chromosomal location of the genes that control the synthesis of gliadins and glutenins, the two major groups of storage protein in the endosperm of wheat (Triticum aestivum). Allelic relationships and genetic linkage between genes are also discussed. The areas that require futher investigation are identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01108632
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 75
    Electronic Resource
    Electronic Resource
    Evaluation of lycosid, micryphantid and linyphiid spiders as predators ofRhopalosiphum padi (Hom.: Aphididae) and their functional response to prey density-laboratory experiments (1993)
    Springer
    BioControl 38 (1993), S. 79-87 
    Details
    ISSN: 1573-8248
    Keywords: wheat ; functional response ; predation ; prey density ; Araneae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Des expériences ont été menées en laboratoire afin de déterminer sur, un plan quantitatif, le rôle, dans l'élimination des populations deRhopalosiphum padi (L.), des principales espèces d'araignées rencontrées sur blé d'hiver en Allemagne:Erigone atra (Blackwall),Lepthyphantes tenuis (Blackwall) etPardosa agrestis (Westring). Leur réponse fonctionnelle à différentes densités de proies a également été étudiée. La présence des araignées provoque une réduction importante, de 34 à 58%, de l'accroissement de la population aphidienne sur blé, par rapport à l'accroissement de cette population en l'absence d'araignées. Les courbes de réponse fonctionnelle pour ces araignées s'attaquant àR. padi semblent de type II caractéristique avec une augmentation de plus en plus faible de la consommation des proies jusqu'à ce que les densités de pucerons atteignent un plateau. Les proies tuées sans être ingérées présentent une courbe de type linéaire.
    Notes: Abstract Laboratory experiments were performed to determine the potential of dominant spider species in winter wheat in Germany,Erigone atra (Blackwall),Lepthyphantes tenuis (Blackwall) andPardosa agrestis (Westring) adults and youngs, in suppressing the population ofRhopalosiphum padi (L.) on wheat plants and their functional response to different aphid densities. The presence of spiders significantly caused between 34 and 58% reduction in aphid population development on wheat plants compared to the aphid population in the absence of spiders. The functional response curves for these spiders as predators ofR. padi seem to descrive a typical type II functional response with the prey consumed increasing to a plateau as aphid densities increased. Prey killed without eating was linear on prey density.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02373142
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 76
    Electronic Resource
    Electronic Resource
    The Septoria Tritici and Stagonospora Nodorum Blotch Diseases of Wheat (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 629-641 
    Details
    ISSN: 1573-8469
    Keywords: wheat ; Triticum spp. ; Septoria tritici ; septoria tritici blotch of wheat Stagonospora nodorum ; stagonospora nodorum blotch of wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008716812259
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 77
    Electronic Resource
    Electronic Resource
    Disease levels in winter wheat, rye and triticale grown on soil artificially inoculated withCephalosporium gramineum (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 701-704 
    Details
    ISSN: 1573-8469
    Keywords: Cephalosporium gramineum ; Cephalosporium stripe ; rye ; susceptibility ; triticale ; wheat ; winter cereals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments with winter cereals grown on soil inoculated withC. gramineum showed that wheat and rye cultivars possess some resistance to the pathogen, while the triticale cultivars were the most susceptible. Higher tolerance of the tested wheat cultivars was connected mainly with slow development of disease symptoms; rye cultivars had, on average, lower percentages of plants infected byC. gramineum. The greatest variation in susceptibility toC. gramineum occurred among the selected cultivars of triticale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01874875
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 78
    Electronic Resource
    Electronic Resource
    Development of apothecia ofTapesia yallundae in contrasting populations selected by fungicides (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 695-699 
    Details
    ISSN: 1573-8469
    Keywords: Pseudocercosporella herpotrichoides ; eyespot ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Apothecia of the eyespot fungus,Tapesia yallundae, were found on 0–18% of straws in plots of wheat stubble in February–March 1994. The fungicides carbendazim, prochloraz or carbendazim plus prochloraz had been applied repeatedly to the same plots in each of the previous 9 years in which successive wheat crops had been grown. The factors most strongly correlated with the incidence of apothecia were the incidence and severity of eyespot in the preceding wheat crop and the frequency of carbendazim-resistant W-type fungus in populations recovered from that wheat crop. Plots treated with carbendazim, which had previously had more disease and more resistance to carbendazim in the pathogen population relative to untreated plots, therefore yielded most apothecia. Plots treated with prochloraz, which had selected for predominantly R-type fungus and decreased eyespot, yielded few apothecia. Single-ascospore isolates were all of the W-type and were more frequently carbendazim-sensitive than expected, except those from plots treated only with carbendazim. None showed decreased sensitivity to prochloraz. The implications of applying fungicides regularly for controlling eyespot on the capability of the eyespot fungus for genetic variation through sexual reproduction are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01874874
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 79
    Electronic Resource
    Electronic Resource
    Effects of feeding lysine-deficient diet on the metabolism of lipids in various tissues of rats (1983)
    Springer
    European journal of nutrition 22 (1983), S. 27-33 
    Details
    ISSN: 1436-6215
    Keywords: wheat ; lysine ; carnitine ; lipids ; mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung 32 abgesetzte männliche Albinoratten wurden in 4 Gruppen eingeteilt, um die Wirkung einer lysinarmen Weizendiät (AW), einer Weizendiät mit 0,4 % Lysin (LW) oder 0,2 % Carnitin (CW) sowie einer Caseindiät auf den Stoffwechsel von Lipiden in verschiedenen Geweben zu untersuchen. Nach 8 Wochen Fütterung der verschiedenen Diäten unter Anwendung der „paired feeding technique“ wurden Veränderungen in den Gesamtlipiden, den Lipidbestandteilen, den einzelnen Fettsäuren und dem Lipidgehalt der Mitochondrien des Herzens, der Skelettmuskeln, der Lungen und des Fettgewebes der Tiere bestimmt. Die lysinarme Weizendiät (AW) bewirkte eine Lipidanreicherung (vor allem an Acylglyzerinen) im Herzen, in der Leber, den Skelettmuskeln sowie eine Lipidabnahme im Fettgewebe. Die Diäten mit 0,4 % Lysin (LW) oder 0,2 % Carnitin (CW) zeitigten die entgegengesetzte Wirkung, wobei CW wirkungsvoller erschien als LW. LW und CW erhöhten die relativen Anteile von C14∶0-, C16∶0- und C16∶1-Fettsäuren und verkleinerten diejenigen von C18∶1-, C18∶2- und C18∶3-Fettsäuren, während die Anteile unter der AW- und Caseindiät ab- bzw. zunahmen. Die Fettsäurezusammensetzung des Fettgewebes war bei allen Gruppen gleich. Die AW-Diät vergrößerte die relativen Anteile von C14∶0- und C20∶4- und verringerte die von C16∶0-, C16∶1- und C18∶3-Fettsäuren in den Lungen. Die AW-Ergänzungsdiäten verringerten die relativen Anteile von C16∶0-, C16∶1-, C18∶3-sowie auch der C18∶1-Fettsäuren. Der Lipidgehalt der Mitochondrien von Leber, Herz, Skelettmuskeln und Lunge verringerte sich unter der AW-Diät und erhöhte sich unter den LW- und CW-Diäten.
    Notes: Summary 32 weanling male albino rats were divided into 4 groups to study the effects of lysine-deficient wheat diet (AW) and AW supplemented with either 0.4 % lysine (LW) or 0.2 % carnitine (CW) as compared to casein diet on metabolism of lipids in various tissues. LW, CW and casein diet groups were pair-fed with AW group. Changes in total lipids, lipid components, individual fatty acids, mitochondrial content in liver, heart, skeletal muscles, lungs and adipose tissue were determined after 8 weeks of feeding. AW diet resulted in accumulation of lipids (mainly acylglycerols) in heart, liver, skeletal muscles and depletion in adipose tissue. The LW and CW diets reversed the effects of AW diet, the CW being more effective than LW diet. The LW and CW diets increased the relative proportion of C 14∶0, C 16∶0, C 16∶1 and decreased that of C18∶1, C18∶2, C18∶3 fatty acids which were decreased and increased, respectively, on the AW and casein diets. The fatty acids composition of adipose tissue was the same in all the groups. The AW diet increased the relative proportions of C 14∶0, C 20∶4 and decreased that of C 16∶0, C 16∶1, C 18∶3 fatty acids in the lungs. Supplemented AW diet decreased the relative proportions of the former group and increased that of the later group including C 18∶1 fatty acid also. The mitochondrial content of liver, heart, skeletal muscles and lungs was decreased on AW and reversed on LW and CW diets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02020782
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 80
    Electronic Resource
    Electronic Resource
    Functional properties of ficoll and their influence on anther culture responses of wheat (1992)
    Springer
    Plant cell, tissue and organ culture 30 (1992), S. 77-83 
    Details
    ISSN: 1573-5044
    Keywords: anther culture ; ficoll ; liquid medium ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of ficoll in liquid culture media have been contradictory in previous reports. The objective of this study was to determine the functional properties of ficoll in potato 4 (P4) liquid induction medium and their influence on anther culture responses of wheat. Ficoll addition significantly (p≤0.01) reduced callus production from the anthers of spring wheat cv. Pavon 76. The reduction was directly related to the concentration of ficoll added within the range of 50 to 200 g l-1 medium. Although the addition of ficoll significantly (p≤0.01) increased the percentage of regenerable calli and the ratio of green vs. albino plants, the final yield of green plants per 100 anthers was significantly lower. Consistent results also were obtained with four other spring wheat genotypes (Chris, Butte 86, WA 6916, and Edwall). Ficoll concentration affected the density, viscosity, and osmolality of the liquid media. The higher medium density caused by ficoll addition increased the percentage of floating calli, as well as the percentage of regenerable calli and the ratio of green vs. albino plants. However, the increased medium viscosity by ficoll addition significantly (p≤0.01) reduced callus production. Ficoll addition also increased medium osmolality, which affected callus production by interacting with the sugar concentration of the induction media. Using response functions, the estimated maltose concentration for maximum callus production was 105 g l-1 for the standard P4 media, compared with 68 g l-1 for the ficoll-containing P4 media. These results clearly demonstrate that ficoll addition to the liquid P4 induction medium containing high sucrose concentration (90 g l-1) is deleterious to the maximum production of green plants from wheat anther culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040004
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 81
    Electronic Resource
    Electronic Resource
    Development of a microtargeting device for particle bombardment of plant meristems (1993)
    Springer
    Plant cell, tissue and organ culture 33 (1993), S. 251-257 
    Details
    ISSN: 1573-5044
    Keywords: ballistic aiming ; glucuronidase ; gold particles ; microprojectiles ; Nicotiana tabacum L. ; tobacco ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene transfer system for meristem cells was developed on the basis of a ballistic approach. In order to meet some important prerequisites for an efficient transfer system, such as for example aiming at small tissues and control of penetration of the microprojectiles, we developed an acceleration system fundamentally different from the usual macroprojectile driven approach. Instead of a macroprojectile, microtargeting uses the law of Bernoulli for accleration of highly uniform-sized gold particles. The system is able to deliver 80% of the particles to an area as small as 150 micron in diameter, which corresponds to the size of a meristem. Microtargeting yields gene delivery (measured as number of transiently GUS expressing cells to up to 3% of the cells exposed in the target area or up to 35 × 103 cells per cm2. Stable transformation of tobacco microcolonies with the microtargeting device was shown to have an efficiency up to one stable transformant per 1000 cells exposed to the shot, or up to one transformant per shot. We perform 4 or 5 shots per min. After 30 to 40 shots, reloading can take up to 2 min. Microtargeting is very flexible and allows for the adjustment of the important parameters to fit the requirements of the respective tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02319009
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 82
    Electronic Resource
    Electronic Resource
    Induction of embryogenic Triticum aestivum L. calli. II. Quantification of organic addenda and other culture variable effects (1987)
    Springer
    Plant cell, tissue and organ culture 10 (1987), S. 115-128 
    Details
    ISSN: 1573-5044
    Keywords: wheat ; somatic embryogenicis ; embryogenic callus ; 3,6-dichloro-o-anisic acid ; 2,4-dichlorophenoxyacetic acid ; 6-furfurylaminopurine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nine experiments were conducted to determine effects of various culture medium addenda on inducation of embryogenic calli from immature embryos of a responsive Triticum aestivum L. genotype (PCYT 10). Effects were quantified by counting somatic embryos (embryoids) per callus. Optimal auxin concentrations to induce and maintain somatic embryogenesis were 3.62 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 9.05 μM 3,6-dichloro-o-anisic acid (dicamba). In general, dicamba permitted formation of significantly more embryoids than 2,4-D. Kinetin (6-furfurylaminopurine) at 2.56 μM or 4.65 μM significantly increased percentage scutellar callus when added to 2,4-D or dicamba-containing medium, respectively. Kinetin at 4.65 μM significantly increased the numbers of embryoids formed when added to medium containing either synthetic auxin. Significantly fewer embryoids formed when cultures were incubated under diffuse light (16-h photoperiod). Casein hydrolysate (200 mg1-1) or L-arginine (0.23 mM) had no effect on numbers of embryoids formed, whereas L-tryptophan (0.20 mM) enhanced such formation with 2,4-D and decreased such formation with dicamba. Two additional experiments generally demonstrated that response to auxin source in the genotypes ND 7532, PCYT 20, Yaqui 50, and Oasis was similar to that in PCYT 10. The higher molar concentration of dicamba required to induce embryogenic callus coupled with more evident embryoid precocious germination and a more rapid rate of tissue necrosis upon extended incubation without subculture suggests that dicamba is metabolized more rapidly than 2,4-D in T. aestivum callus cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035909
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 83
    Electronic Resource
    Electronic Resource
    Induction of embryogenicTriticum aestivum L. calli. II. Quantification of organic addenda and other culture variable effects (1988)
    Springer
    Plant cell, tissue and organ culture 12 (1988), S. 97-110 
    Details
    ISSN: 1573-5044
    Keywords: wheat ; somatic embryogenesis ; embryogenic callus ; 3,6-dichloro-o-anisic acid ; 2,4-dichlorophenoxyacetic acid ; 6-furfurylaminopurine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nine experiments were conducted to determine effects of various culture medium addenda on induction of embryogenic calli from immature embryos of a responsiveTriticum aestivum L. genotype (PCYT 10). Effects were quatified by counting somatic embryos (embryoids) per callus. Optimal auxin concentrations to induce and maintain somatic embryogenesis were 3.62 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 9.05 μM 3,6-dichloro-o-anisic acid (dicamba). In general, dicamba permitted formation of significantly more embryoids than 2,4-D. Kinetin (6-furfurylaminopurine) at 2.56 μM or 4.65 μM significantly increased percentage scutellar callus when added to 2,4-D or dicamba-containing medium, respectively. Kinetin at 4.65 μM signficantly increased the numbers of embryoids formed when added to medium containing either synthetic auxin. Significantly fewer embryoids formed when cultures were incubated under diffuse light (16-h photoperiod). Casein hydrolysate (200 mgl-1) or L-arginine (0.23 mM) had no effect on numbers of embryoids formed, whereas L-tryptophan (0.20 mM) enhanced such formation with 2,4-D and decreased such formation with dicamba. Two additional experiments generally demonstrated that response to auxin source in the genotypes ND 7532, PCYT 20, Yaqui 50, and Oasis was similar to that in PCYT 10. The higher molar concentration of dicamba required to induce embryogenic callus coupled with more evident embryoid precocious germination and a more rapid rate of tissue necrosis upon extended incubation without subculture suggests that dicamba is metabolized more rapidly than 2,4-D inT. aestivum callus cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043113
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 84
    Electronic Resource
    Electronic Resource
    Isozyme modifications during morphogenesis of callus from barley and wheat (1988)
    Springer
    Plant cell, tissue and organ culture 12 (1988), S. 299-304 
    Details
    ISSN: 1573-5044
    Keywords: callus ; plant regeneration ; isozymes ; wheat ; barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus cultures fron non-organogenic, young and one-year old, and morphogenic calli were used to assess the value of isozymes analysis for the prediction of morphogenic capacity by studying esterase, peroxidase and acid phosphatase. Basic isozyme patterns of each enzyme for the callus were retained in all the callus stages and in the callus which has differentiated into shoots. With the development of shoot and/or root some conspicuous isozymes appeared for esterase and acid phosphatase and some disappeared for peroxidase. As the isozyme changes became apparent only after shoot or root initiation these enzymes could not be used as markers to distinguish between morphogenic and non-morphogenic calli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034372
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 85
    Electronic Resource
    Electronic Resource
    Modulation of somatic embryogenesis in early and late-stage embryos of wheat (Triticum aestivum L.) under the influence of (±)-abscisic acid and its analogs (1989)
    Springer
    Plant cell, tissue and organ culture 18 (1989), S. 55-69 
    Details
    ISSN: 1573-5044
    Keywords: wheat ; Triticum aestivum ; ABA ; ABA analogs ; somatic embryogenesis ; precocious germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Zygotic embryos from ten spring wheat (Triticum aestivum L.) genotypes were tested for embryogenic callus induction in the presence or absence of externally supplied (±)-abscisic acid (ABA) and two of its analogs, methyl abscisate and methyl epoxy-beta-ionylideneacetate. (±)-ABA and its analogs suppressed precocious germination of cultured late-stage embryos and promoted embryogenic callus induction. A significantly greater number of plants was regenerated from calli induced in the presence of ABA and ABA analogs. Early-stage embryos when cultured in the presence of (±)-ABA showed a negative response. Possible roles of ABA with respect to the expression of somatic embryogenesis are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033465
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 86
    Electronic Resource
    Electronic Resource
    Zinc deficiency and the formation of white structures in immature embryo cultures of wheat (Triticum aestivum L.) (1991)
    Springer
    Plant cell, tissue and organ culture 24 (1991), S. 9-12 
    Details
    ISSN: 1573-5044
    Keywords: culture medium ; embryo culture ; microelements ; wheat ; zinc ; zinc deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of microelements on the induction of embryogenic callus from epiblast and scutellum of wheat (Triticum aestivum L.) embryos was studied by the sequential omission of each of the microelements from Murashige & Skoog medium. Omission of iron caused a marked decrease in yield and poor shoot formation from embryogenic callus. The yield of embryogenic callus on medium without added manganese was also reduced. Omission of boron, copper-cobalt, iodine, and molybdenum had little effect on the induction of embryogenic epiblast callus. By contrast there was a marked increase in the formation of white structures on the medium without any microelements or, specifically without addition of zinc. Since the formation of typical embryoids of wheat is associated with the formation of white structures, our result highlights the importance of certain microelements on somatic embryogenesis of wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00044258
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 87
    Electronic Resource
    Electronic Resource
    Wheat anther culture: effect of genotype and environmental conditions (1991)
    Springer
    Plant cell, tissue and organ culture 24 (1991), S. 233-236 
    Details
    ISSN: 1573-5044
    Keywords: anther culture ; cultivars ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-two cultivars and lines of winter and spring wheat (Triticum aestivum L.) were studied, most for the first time, for their anther culture response. The response was genotype dependent. Plants grown in the field gave higher callus induction frequency than those grown in the greenhouse and the controlled environment chamber. Donor plants grown in a season of low drought stress as compared to a season of severe drought stress resulted in a higher frequency of callus induction. Spherical microcalli were observed in two wheat genotypes in some of only those anthers that were placed with only one loculus in contact with the medium. Wheat lines that were more responsive to anther culture were identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033482
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 88
    Electronic Resource
    Electronic Resource
    La localisation, le comportement et les relations «Proie-prédateur» chezCoccinella septempunctata dans un champ de blé (1991)
    Springer
    BioControl 36 (1991), S. 213-225 
    Details
    ISSN: 1573-8248
    Keywords: Coccinella septempunctata ; distribution verticale ; comportement ; alimentation ; puceron ; blé ; Coccinella septempunctata ; vertical distribution ; behaviour ; feeding ; aphid ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary Observations were made in a wheat field during daylight hours to determine how the vertical distribution of adults and larvae ofCoccinella septempunctata changed with season and in response to aphid populations on which this coccinellid feeds. In early spring, this thermophilic coccinellid (larvae and adults) remained close to the ground and on the lower part of tiller leaves where it was highly active. Adults frequently groomed their head appendages. By June, most adults and larvae were found on the upper parts of tillers, the area preferred by their aphid prey. The beetles remained close to such sites and hence their vertical distribution corresponded to that of the aphids on which they fed. The fact that the beetles appeared to spend most of their time resting and moving within wheat crops, and little time feeding, raises the question of how the larvae and adults satisfy their dietary needs.
    Notes: Abstract Des adultes et des larves deC. septempunctata ont été observés individuellement dans un champ de blé afin de préciser, au cours de la journée et en fonction de la saison, leur distribution sur le végétal, la durée des principales séquences de leur comportement et leurs relations avec les pucerons. Au début du printemps, cette espèce thermophile est présente sur le sol et sur les feuilles basses des talles où elle est très mobile. Les adultes font très fréquemment la toilette des appendices de la tête. En juin, la population larvaire et imaginale se regroupe sur la partie supérieure des talles où se trouvent les pucerons. A cette époque, l'immobilité domine. Ces variations de la distribution verticale sont confirmées par la position sur le végétal des proies capturées. Les données suggèrent que, dans les champs, cette coccinelle consacre peu de temps à son alimentation. Le faible nombre de proies ingérées pose le problème de la couverture de ses besoins trophiques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02374557
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 89
    Electronic Resource
    Electronic Resource
    Genetic aspects of wheat gliadin proteins (1978)
    Springer
    Biochemical genetics 16 (1978), S. 831-853 
    Details
    ISSN: 1573-4927
    Keywords: Triticum aestivum L. ; wheat ; storage proteins ; gliadins ; gel electrophoresis ; endosperm ; dominant genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Inheritance of gliadin components unique to three different varieties of common wheat (Triticum aestivum L.) was studied in F1 and F2 seeds of intervarietal crosses using protein patterns obtained by polyacrylamide gel electrophoresis in aluminum lactate buffer (pH 3.2). The patterns of F1 seeds of the crosses Cheyenne × Justin and INIA 66R × Justin evidenced all the bands present in the patterns of the parents; band intensities reflected gene dosage levels dependent on whether the contributing parent was maternal or paternal in accordance with the triploid nature of endosperm tissue. Most of the gliadin components examined segregated in accordance with control by a single dominant gene, but in two instances single bands in the one-dimensional electrophoretic patterns segregated in the F2 as expected if controlled by two genes. A method of two-dimensional electrophoresis was developed that resolved these apparently single bands into two components each, which could segregate independently. Linkage analysis provided evidence of codominant alleles and closely linked genes coding for gliadin protein components in both coupling and repulsion situations. The gliadin protein components seem to be coded for by clusters of genes located on chromosomes of homoeologous groups 1 and 6 in hexaploid wheats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484739
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 90
    Electronic Resource
    Electronic Resource
    Isozymes in wheat-barley hybrid derivative lines (1981)
    Springer
    Biochemical genetics 19 (1981), S. 237-254 
    Details
    ISSN: 1573-4927
    Keywords: wheat ; barley ; addition lines ; isozymes ; genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Zymogram analysis was used to identify the barley chromosomes that carry the structural genes for particular isozymes. Wheat, barley, and wheatbarley hybrid derivative lines (which contained identified barley chromosomes) were tested by gel electrophoresis for isozymes of particular enzymes. It was found that barley chromosome 4 carries structural genes for acid phosphatase and β amylase isozymes, barley chromosome 5 carries genes for phosphoglucose isomerase and malate dehydrogenase isozymes, and that barley chromosome 2 carries a gene for at least one glucose-6-phosphate dehydrogenase protomer. These results reinforce previous conclusions that barley chromosome 4 shows homoeology with wheat chromosome group 4 and that barley chromosome 5 shows homoeology with wheat chromosome group 1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00504271
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 91
    Electronic Resource
    Electronic Resource
    Genetic control of a novel series of trypsin inhibitors in wheat and its relatives (1987)
    Springer
    Biochemical genetics 25 (1987), S. 591-602 
    Details
    ISSN: 1573-4927
    Keywords: trypsin inhibitor ; homoeoloci ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The aneuploids of Chinese Spring wheat have been used to locate the genes(Ti-2) coding for a novel series of trypsin inhibitors to the long arms of the homoeologous group 5 chromosomes. Three allelic variants at the 5D locus were detected in a limited survey among wheat varieties, but no variation at the loci on either chromosome 5A or chromosome 5B was detected. Homoeoloci were found in a number of alien relatives, and in the majority of cases, these were present on the group 5 homoeologue. However, inAegilops umbellulata, theTi-U2 locus was located on a chromosome presumed to belong to homoeologous group 1. NoHordeum vulgare orH. chilense Ti-2 gene was expressed in a wheat background. This new marker will be especially useful as a screening mechanism for nullisomy of chromosome 5B in work aimed at introgression of alien chromatin into wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00554360
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 92
    Electronic Resource
    Electronic Resource
    Polymorphism and inheritance of gliadin components controlled by chromosome 6A of spring durum wheat (1988)
    Springer
    Biochemical genetics 26 (1988), S. 693-703 
    Details
    ISSN: 1573-4927
    Keywords: wheat ; gliadins ; gel electrophoresis ; evolution ; genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate,pH 3.1) and two-dimensional (first dimension, Al-lactate,pH 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Analysis of hybrids has shown that all components of the α zone of gliadin spectra are inherited together as blocks and are, probably, coded for by a cluster of tightly linked genes located on chromosome 6A. Fourteen variants of gliadin blocks have been identified, which can be classified into five families on the basis of component composition. All families but one have analogues among chromosome 6A-controlled blocks of bread wheat. The results indicate that some of the genome A diploid genotypes that were ancestors of durum wheats were also ancestors of bread wheats and that polyploid wheats were produced by repeated allopolyploidization events, as has been suggested earlier.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395516
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 93
    Electronic Resource
    Electronic Resource
    Neocentric activity of rye 5RL chromosome in wheat (2000)
    Springer
    Chromosome research 8 (2000), S. 543-554 
    Details
    ISSN: 1573-6849
    Keywords: genomic in-situ hybridization ; meiosis ; neocentromere ; rye ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The neocentric activity of a constriction located on the long arm of rye 5R chromosome (5RL) was analysed. It is not observed in normal rye but it is unusually stretched in bivalents involving 5RL telosomes in wheat–ditelosomic 5RL addition lines. In 20% of metaphase I cells, the 5RL bivalent presents the centromeres oriented to one pole and the constrictions oriented towards the opposite pole with a strong tension. In 5% of the cells, the constriction was able to orient the bivalent to the poles without tension in the centromeres. Sister chromatid cohesion, which is one of the distinct features of centromeric function, is persistent at the constriction in delayed 5RL chromosomes at anaphase I. Neither the elongation of the constriction nor the neocentric activity was observed at second meiotic division or mitosis. FISH studies showed that the 5RL constriction lacked detectable quantities of two repetitive DNA sequences, CCS1 and the 180-bp knob repeat, present at cereal centromeres and neocentromeres, respectively. We propose that, under special conditions, such as the wheat background, the normally non-centromeric DNA present at this region of 5RL acquires a specific chromatin structure, differentiated as an elongated constriction, which is able to function as a centromere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009275807397
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 94
    Electronic Resource
    Electronic Resource
    Genetic research as the valid base of strategies for breeding rust resistant wheats (1996)
    Springer
    Genetica 97 (1996), S. 243-254 
    Details
    ISSN: 1573-6857
    Keywords: durable resistance ; induced resistance ; leaf rust ; rust resistance ; stem rust ; stripe rust ; wheat ; wheat breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It is known that few wheat cultivars maintain their resistance to rust diseases for a long period of time, particularly when crop populations become genetically more uniform. A number of genetically diverse, so far unexploited, sources of rust resistance in the natural as well as mutagenized population of wheat cultivars were identified. Several of these genes were placed in agronomically superior well-adapted backgrounds so that they could be used as pre-breeding stocks for introducing genetic diversity for resistance in a crop population. Some of these stocks when employed as parents in several cross combinations in a breeding programme have generated a number of promising cultivars with diversity for resistance. Many presently grown wheats in India, near-isogenic lines each with Lr14b, Lr14ab, Lr30 and certain international cultivars were identified as possessing diverse sources of adult plant resistance (APR) to leaf rust. Prolonged leaf rust resistance in some of the Indian cultivars was attributed to the likely presence of Lr34 either alone or in combination with other APR components. Tests of allelism carried out in certain cultivars that continue to show adequate levels of field resistance confirm the presence of Lr34, which explains the role that this gene has played in imparting durability for resistance to leaf rust. Also, Lr34 in combination with other APR components increases the levels of resistance, which suggests that combination of certain APR components should be another important strategy for breeding cultivars conferring durable and adequate levels of resistance. A new adult plant leaf rust resistance source that seems to be associated with durability in ‘Arjun’ has been postulated. Likewise, cultivars possessing Sr2 in combination with certain other specific genes have maintained resistance to stem rust. Further, non-specific resistances that were transferred across widely different genotypes into two of the popular Indian wheats provided easily usable materials to the national breeding programmes for imparting durable resistance to stripe rust.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00055311
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 95
    Electronic Resource
    Electronic Resource
    Characterization of the translocated chromosome using fluorescencein situ hybridization and random amplified polymorphic DNA on twoTriticum aestivum—Thinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus (1996)
    Springer
    Chromosome research 4 (1996), S. 583-587 
    Details
    ISSN: 1573-6849
    Keywords: in situ hybridization ; intermediate wheatgrass ; translocation ; virus resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescencein situ hybridization (FISH) was used to determine the breakpoint of the translocation chromosome in two bread wheat (Triticum aestivum) germplasm lines withThinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, genome-specific random amplified polymorphic DNA (RAPD) markers were used to ascertain the genomic sources of theTh. intermedium chromosomes carrying the WSMV or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from induced homoeologous pairing by using theph1b mutant, had a translocation chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, and the entire 4ES ofTh. intermedium. The BYDV-resistant translocation line, TC14, derived from tissue culture, had a very short distal segment of 7StL fromTh. intermedium terminally attached to 56% of the proximal 7DL. These observations indicate that translocations in these wheat germplasm lines did not involve centromeric breaks and fusion but were a result of homoeologous chromosome recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02261721
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 96
    Electronic Resource
    Electronic Resource
    Meiosis of Wheat × Lymegrass Hybrids (1998)
    Springer
    Chromosome research 6 (1998), S. 339-344 
    Details
    ISSN: 1573-6849
    Keywords: Leymus ; meiosis ; molecular cytogenetics ; wheat ; wide-hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Meiosis was examined in pollen mother cells of F1 hybrids made from crosses between wheat (Triticum aestivum) and lymegrass (Leymus arenarius and L. mollis). Fluorescence genomic in situ hybridization detected pairing between wheat and lymegrass chromosomes during prophase I and metaphase I. Such pairing, when resulting in bivalent formation, was likely to yield correct disjunction, and hence intergenomic recombination could be incorporated into the gametes. Bivalents in these hybrids, however, were more frequently formed between chromosomes of the same parental origin. Univalents were common, whereas multivalents were not clearly detected. Meiotic behaviour in some cells was not totally aberrant, and this may have accounted for the presence of normal pollen. The results are discussed in relation to intergenomic pairing, meiotic behaviour in wide-hybrids and genome relationships, including the Leymus genome origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009281911723
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 97
    Electronic Resource
    Electronic Resource
    The influence of anther cold pretreatments and donor plant genotypes on in vitro androgenesis in wheat (Triticum aestivum L.) (1984)
    Springer
    Plant cell, tissue and organ culture 3 (1984), S. 69-79 
    Details
    ISSN: 1573-5044
    Keywords: anther culture ; androgenesis ; cold pretreatment ; microspore ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cold pretreatments applied to excised anthers in liquid ‘potato 2’ medium proved to be unnecessary. Generally, cold pretreatments inhibited anther response and productivity as the duration was lengthened or as the pretreatment temperature was lowered. There were significant differences in response attributed to the anther donor genotype. Green and albino plants as well as ‘roots only’ have been regenerated from the spring wheat cultivars Sinton, Neepawa, Pitic 62 and DW 50. Most plants were haploid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035922
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 98
    Electronic Resource
    Electronic Resource
    Identity and frequency of occurrence ofTrichoderma spp. in roots of wheat and rye-grass in Western Australia and their effect on root rot caused byGaeumannomyces graminis var.tritici (1988)
    Springer
    Plant and soil 109 (1988), S. 93-101 
    Details
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis var.tritici ; rye grass ; Trichoderma hamatum ; T. harzianum ; T. koningii ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Trichoderma hamatum, T. harzianum andT. koningii were isolated from wheat and rye-grass roots from a field in Western Australia. Frequency of occurrence ofTrichoderma spp. was higher on roots subjected to washing only, for both wheat and rye-grass than the roots which were surface-sterilized with 0.6% or 1.25% NaOCl.Trichoderma spp. were recovered at a higher frequency on PDA amended with lactic acid (pH 4.5) than on PDA alone (pH 5.6) or PDA with streptomycin. In general,Trichoderma spp. were isolated at a higher frequency from roots of wheat than that of rye-grass.T. hamatum occurred at a higher frequency in rye-grass roots than in wheat, whereasT. harzianum was more common in roots of wheat than in rye-grass, especially in seedling and milky ripe stages.T. koningii was recovered at a higher frequency from roots at seedling stage of rye-grass than wheat, the reverse being true at tillering stage.T. koningii was not recovered from roots of either host in any sampling when they were surface sterilized with 1.25% NaOCl. The take-all fungus was isolated from wheat and rye-grass roots more frequently at tillering and stem extension stages than others. It was severely pathogenic to both hosts in sterilized and non-sterilized soil. Addition of lactic acid, HCl or streptomycin to PDA did not affect the growth of theTrichoderma spp. tested, but the growth was slower on Martin's medium than on other media. In generalT. harzianum andT. koningii grow faster thanT. hamatum. The growth of the three species were not different at 20 and 25°C, but at 15°c growing of all species was significantly reduced. Incorporation of lactic acid into PDA prevented the bacterial growth in all treatments. Streptomycin too reduced but to a lesser degree than lactic acid. Surface sterilization with NaOCl decreased the recovery of both bacteria and fungi. T. hamatum andT. koningii reduced the mortality of wheat and rye-grass plants inoculated with the take-all fungus in sterilized and non-sterilized soil, whereT. harzianum did not protect wheat or rye-grass from infection by the take-all fungus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02197585
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 99
    Electronic Resource
    Electronic Resource
    Toxicity to plants and animals of an undescribed, neosolaniol monoacetate-producingFusarium species from soil (1989)
    Springer
    Plant and soil 114 (1989), S. 75-83 
    Details
    ISSN: 1573-5036
    Keywords: Fusarium camptoceras ; Fusarium sp. ; Medicago spp. ; neosolaniol monoacetate ; phytotoxicity ; toxicity ; trichothecenes ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The toxicity of 12 South African isolates of an undescribedFusarium sp. from soil and other substrates was tested in plants and animals. TheFusarium sp. resemblesF. camptoceras but differs in several respects. Two reference cultures ofF. camptoceras were therefore included in the toxicity tests. An isolate ofF. graminearum Gr 1, a known pathogen of annualMedicago spp. (medics) and wheat, was also included in the plant tests. Maize cultures of all theFusarium sp. isolates and oneF. camptoceras isolate were toxic to ducklings. Ethyl acetate extracts of cultures of nine of ten isolates of theFusarium sp. were dermotoxic to rabbit skin. In soil infested with sand-bran inoculum of the fungi, allFusarium sp. isolates, oneF. camptoceras isolate andF. graminearum Gr 1 caused significant (P=0.05) mortality of medics, whereas onlyF. graminearum Gr 1 caused mortality of wheat plants. Seven isolates of theFusarium sp. caused stunting of medic and wheat plants as well as discolouration, necrosis and die-back of the tap root of medics and the primary roots of wheat. The fungus could, however, not be isolated from these necrotic roots. In contrast,F. graminearum Gr 1 that caused discolouration of medic roots and typical crown rot symptoms of wheat, was readily isolated from affected roots and crowns. Maize cultures of isolates of theFusarium sp. and ofF. camptoceras were chemically analysed for neosolaniol monoacetate (NMA), diacetoxyscirpenol (DAS) and T-2 toxin. NMA was detected at levels ranging from 310 to 2060 ng g−1 in 10 of theFusarium sp. isolates, but not in theF. camptoceras isolates. The mortality of medic plants and the NMA yields of isolates of theFusarium sp. in maize cultures were significantly correlated (r=0.84,P〈0.05). A solution containing 10 mg litre−1 of pure NMA was only slightly toxic to ducklings. Exposure of seeds to this solution had no effect on medics and wheat, but exposure of seedlings caused marked mortality in medics and reduction of shoot length in wheat. A solution containing 100 mg litre−1 of NMA was acutely toxic to ducklings, but had no effect on medic and wheat plants when added to soil. Phytotoxic effects on medic and wheat plants were obtained with a concentration of 5000 ng NMA g−1 soil. In all tests, the phytotoxic effects were more drastic in medics than in wheat: medics were killed, whereas wheat seedlings were stunted. This is the first report of the phytotoxicity of NMA to medics and wheatin vivo and probably also the first report of the phytotoxic effects of a pure trichothecene added to soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02203084
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 100
    Electronic Resource
    Electronic Resource
    Toxin production by a rhizobacterialPseudomonas sp. that inhibits wheat root growth (1989)
    Springer
    Plant and soil 114 (1989), S. 269-278 
    Details
    ISSN: 1573-5036
    Keywords: deleterious ; inhibitory bacteria ; pseudomonads ; rhizobacteria ; toxin ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We studied the production of a toxin inhibitory to both winter wheat (Triticum aestivum L.) root growth andEscherichia coli that was produced by a rhizobacterial pseudomonad. Of several carbon sources tested, the most rapid growth and highest toxin concentrations were obtained with glucose, glycerol, or trehalose. Toxin production was repressed with L-cysteine as the nitrogen source. Toxin was produced during the late exponential and early stationary phase of growth by the bacterium and, contrary to studies with other toxins, was unaffected by Fe and P concentrations in the growth medium. Toxin production by the bacterium was the same at growth temperatures of 25 and 15°C while it produced less at 5°C. If the bacterium was able to grow, it produced toxin. No compound tested induced an increase in toxin production indicating toxin production is constitutive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02220807
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...