Photosynthetica 1999, 36(3):433-440 | DOI: 10.1023/A:1007080204112

Specific Degradation of D1 Protein during Exposure of Thylakoid Membranes to High Temperature in the Dark

A.K. Singh, G.S. Singhal

Exposure of thylakoid membranes to high temperature in dark leads to the degradation of D1 protein. Maximum degradation of D1 protein occurred at 45 °C. Using N-terminal specific D1 antibody, a 23 kDa fragment of D1 protein was detected. The degradation of D1 protein could be prevented both by radical scavengers and inhibitors of serine protease and metallo-protease. These results suggest that degradation of D1 protein during exposure of thylakoid membranes to high temperature in dark is catalyzed by protease.

Additional key words: antibody; polyacrylamide gel electrophoresis; protease; Triticum aestivum; wheat

Published: August 1, 1999  Show citation

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Singh, A.K., & Singhal, G.S. (1999). Specific Degradation of D1 Protein during Exposure of Thylakoid Membranes to High Temperature in the Dark. Photosynthetica, 36(3), 433-440. doi: 10.1023/A:1007080204112
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