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Expression of growth hormone receptor by immunocytochemistry in rat molar root formation and alveolar bone remodeling

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Summary

Growth hormone (GH) may regulate tooth formation and bone remodeling associated with tooth eruption. This study reports the distribution of growth hormone receptor/binding protein in developing rat molars and adjacent alveolar bone by immunocytochemistry using well-characterized anti-growth hormone receptor monoclonal antibodies. These tissues represent an excellent model for studying the ontogenic changes that occur in odontogenic and osteogenic cells, as these cells are found in linear arrays displaying the various stages of morphological and functional differention, and differentiated function. Immunoreactivity was first seen in precementoblasts in contact with the epithelial root sheath, and preodontoblasts. However, growth hormone receptor immunoreactivity was associated primarily with the cytoplasm of odontogenic and osteogenic cells forming their respective matrices. Thus, cementoblasts and odontoblasts at sites of new matrix formation showed intense immunoreactivity whereas cementocytes and mature odontoblasts at later stages of tooth development were nonreactive. Osteoblasts engaged in intramembranous ossification in the alveolar bone were positive, although osteocytes and endosteal cells were immunonegative. Osteoclasts at sites of alveolar bone remodeling resorption were also immunopositive. These patterns of receptor expression parallel the ontogenic sequences of odontogenic and osteogenic cells and suggest that GH promotes the functional state of these cells. Our results also imply that GH may influence differentiation or differentiated functions associated with odontogenesis, osteogenesis, and bone remodeling independent of systemic insulin-like GF-I.

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Authors and Affiliations

  1. Division of Oral Biology and Oral Pathology, Department of Dentistry, The University of Queensland, Physiology Building, 4072, Queensland, Australia

    Chayvis Z. Zhang, W. G. Young, H. Li & A. M. Clayden

  2. Department of Physiology and Pharmacology, The University of Queensland, 4072, Queensland, Australia

    J. Garcia-Aragon & M. J. Waters

Authors
  1. Chayvis Z. Zhang
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  2. W. G. Young
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  3. H. Li
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  4. A. M. Clayden
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  5. J. Garcia-Aragon
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  6. M. J. Waters
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Zhang, C.Z., Young, W.G., Li, H. et al. Expression of growth hormone receptor by immunocytochemistry in rat molar root formation and alveolar bone remodeling. Calcif Tissue Int 50, 541–546 (1992). https://doi.org/10.1007/BF00582170

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  • DOI: https://doi.org/10.1007/BF00582170

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