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Occurrence and expression of acid phosphatase of Hymenoscyphus ericae (Read) Korf & Kernan, in isolation or associated with plant roots

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Summary

The activity of acid phosphatase produced in pure culture by the endomycorrhizal fungus Hymenoscyphus ericae (Read) Korf & Kernan (H. ericae LPA 2) was inhibited by high phosphorus levels, alkaline pH, fluoride, molybdate and mannosidase, and activated by concanavalin A. Over 80% of the enzyme activity was due to two wall-bound acid phosphatase isozymes with the characteristics of mannose-rich glycoproteins. Antiserum was raised against the major, low-molecular-weight wall isozyme and its activity tested by immunoblotting and ELISA. The antiserum cross reacted 100% with exocellular (excreted) and 28% with cytoplasmic cellular fractions of H. ericae (LPA 2) cultures, and showed high reactivity with other strains of H. ericae but not with fungal isolates from Erica hispidula L. or E. mauritanica L. Ultrastructural localization of acid phosphatase by cytoenzymology and indirect immunogold labelling confirmed its association with the fungal wall in pure culture and showed that the influence of a high phosphorus level, fluoride and molybdate is through inactivation of the enzyme. Intense acid phosphatase activity, sensitive to the latter inhibitors, was also present on external hyphae growing over a host or non-host root but it was weak or absent from intracellular hyphae where these developed within a host root. Indirect immunolabelling confirmed that this acid phosphatase was of fungal origin and that the specific inhibitory effect of host cells is due to inactivation of the enzyme rather than repression of its synthesis. Possible implications of fungal acid phosphatase in ericoid endomycorrhizal infection processes are discussed together with mechanisms that may be regulating the enzyme activity.

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Authors and Affiliations

  1. Laboratoire de Phytoparasitologie, INRA-CNRS, Station de Génétique et d'Amélioration des Plantes, INRA, BV 1540, F-21034, Dijon Cedex, France

    M. C. Lemoine, V. Gianinazzi-Pearson & S. Gianinazzi

  2. Department of Botany, University of Witwatersrand, Private Bag 3, Wits 2050, Johannesburg, South Africa

    C. J. Straker

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  1. M. C. Lemoine
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  2. V. Gianinazzi-Pearson
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  3. S. Gianinazzi
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  4. C. J. Straker
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Lemoine, M.C., Gianinazzi-Pearson, V., Gianinazzi, S. et al. Occurrence and expression of acid phosphatase of Hymenoscyphus ericae (Read) Korf & Kernan, in isolation or associated with plant roots. Mycorrhiza 1, 137–146 (1992). https://doi.org/10.1007/BF00203287

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