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Immunocytochemical localization of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum

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Abstract

Cells of Methanobacterium thermoautotrophicum were fixed with glutaraldehyde, sectioned and labeled with antibodies against the β subunit of component C (=methyl-CoM reductase) of methyl-CoM reductase system and with colloidal gold-labeled protein A. It was found that the gold particles were located predominantly in the vicinity of the cytoplasmic membrane, when the cells were grown under conditions where methyl-CoM reductase was not overproduced. This finding confirms the recent data obtained with Methanococcus voltae showing via the same immunocytochemical localization technique that in this organism methyl-CoM reductase is membrane associated.

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Author notes

  1. H. C. Aldrich

    Present address: Department of Microbiology and Cell Science, University of Florida, 32611, Gainesville, FL, USA

Authors and Affiliations

  1. Fachbereich Biologie-Mikrobiologie, Philipps-Universität Marburg, Karl-von-Frisch-Strasse, D-3550, Marburg, Federal Republic of Germany

    H. C. Aldrich, D. B. Beimborn, M. Bokranz & P. Schönheit

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  1. H. C. Aldrich
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  2. D. B. Beimborn
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  3. M. Bokranz
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  4. P. Schönheit
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Aldrich, H.C., Beimborn, D.B., Bokranz, M. et al. Immunocytochemical localization of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum . Arch. Microbiol. 147, 190–194 (1987). https://doi.org/10.1007/BF00415283

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  • DOI: https://doi.org/10.1007/BF00415283

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