ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Articles  (183)
  • resistance  (105)
  • RAPD  (78)
  • Springer  (183)
  • American Chemical Society
  • 1995-1999  (183)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (183)
  • 1
    Electronic Resource
    Electronic Resource
    Genetics of soybean-Heterodera glycines system (1995)
    Springer
    Integrated pest management reviews 1 (1995), S. 65-69 
    Details
    ISSN: 1572-9745
    Keywords: Glycine max ; Heterodera glycines ; inheritance ; resistance ; soybean ; soybean cyst nematode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetics of resistance to soybean cyst nematode (SCN), Heterodera glycines Ichinohe is very complex. Crosses involving PI 437654, which is resistant to all races of cyst nematodes with other sources of resistance (Peking, PI 88788, and PI 90763) indicated that resistance to race 3 was controlled by four genes, two of which were dominant resistance genes and the other two were recessive resistance genes. For race 5, a four gene model with two recessive and two dominant resistance genes in epistasis has been proposed. For race 14, the results suggested a three gene model with one dominant and two recessive alleles. Several other plant introductions have been isolated which have different genes conditioning resistance. Most of the currently grown soybean varieties derived resistance from Peking and/or PI 88788. Resistance to SCN in these soybean varieties has broken down because of the emergence of several new races and populations of SCN. The use of PI 437654 or Hartwig and other plant introductions with different genes for resistance will broaden genetic diversity and stabilize yield.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00140334
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The Components and Deployment of Resistance to Cassava Mosaic Virus Disease (1998)
    Springer
    Integrated pest management reviews 3 (1998), S. 209-224 
    Details
    ISSN: 1572-9745
    Keywords: cassava ; Manihot esculenta ; Africa ; cassava mosaic virus disease ; whitefly vector (Bemisia tabaci) ; control ; varietal diversity ; resistance ; deployment ; durability ; breakdown ; phytosanitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cassava mosaic virus disease (CMD) is prevalent and causes serious losses in cassava (Manihot esculenta) in southern India and in many parts of sub-Saharan Africa. The disease is caused by viruses of the Geminiviridae that are transmitted by the whitefly Bemisia tabaci and disseminated in the stem cuttings used routinely for propagation. The main approach to control is through the use of virus-resistant varieties, but suitable ones are not always available and susceptible varieties are still widely grown. This explains why CMD continues to be a problem in many areas. CMD-resistant varieties have several features which are considered in this review:- • They are not readily infected, even when exposed to large amounts of vector-borne inoculum. • When infected they develop symptoms that tend to be inconspicuous and not associated with obvious deleterious effects on growth or yield. Moreover, the symptoms become even less conspicuous as growth proceeds and plants may eventually recover and become symptomless. • Infected plants support a low virus content and they are likely to be a poor source of inoculum from which further spread can occur. • Virus is not fully systemic within infected plants which can be a source of uninfected planting material when stem cuttings are collected for further propagation. This phenomenon is referred to as ‘reversion’ and it has an important ‘cleansing effect’ in restricting or preventing the progressive deterioration in health status that would otherwise occur during successive cycles of vegetative propagation. The available information on the different components of resistance is discussed and it is concluded that they are inter-related features of the same basic mechanism that restricts virus entry, replication and movement within the host. It is argued that the effectiveness and durability of virus-resistant varieties are likely to be influenced by the way in which they are deployed. However, this topic has received little attention from researchers and there is continuing uncertainty on the effects of CMD on the yield of resistant varieties and on the role of phytosanitation. This involves the use of virus-free planting material and the removal (roguing) of any additional diseased plants that occur. Some consider that these procedures complement the use of virus-resistant varieties and should be adopted, whereas others argue that they are unnecessary and inappropriate. It is concluded that there is considerable scope for utilizing resistant varieties more widely and more effectively than at present, but in doing so it is important to avoid eroding the genetic diversity that is currently such a marked feature of cassava cultivation in Africa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009626806156
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Light induced bio-control of potato storage pathogens in vitro (1998)
    Springer
    Potato research 41 (1998), S. 143-153 
    Details
    ISSN: 1871-4528
    Keywords: resistance ; Solanum tuberosum L. cultivars ; glycoalkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of freeze dried potato powder derived from tubers (cvs Désirée and Epicure) exposed to light as a culture medium on the growth, number and spore size of nine fungal pathogens was determined in vitro with particular emphasis on the role of glycoalkaloids. Greater reductions in pathogen growth reflected increased exposure to light of tubers prior to freeze drying. High correlations (% R2 adj) were obtained between glycoalkaloid concentration and growth rate of the majority of pathogens tested. Where spores were produced, numbers were in general significantly less when cultured on freeze dried powder derived from tubers exposed to light. No effects on spore size ofFusarium species were recorded, but spore length of remaining pathogens was reduced and spore width increased, with the exception ofC. coccodes where spore length and width was increased following culture onto freeze dried powder derived from tubers exposed to light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358437
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Inheritance of extreme resistance to potato virus Y in potato (1995)
    Springer
    Potato research 38 (1995), S. 199-210 
    Details
    ISSN: 1871-4528
    Keywords: PVY ; resistance ; progeny tests ; inheritance ; potato breeding ; Solanum tuberosum L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Segregation for extreme resistance to PVY was evaluated in progenies derived from crossing two extremely resistant potato clones with parents differing in resistance. Resistance was evaluated after mechanical inoculation with PVYO and PVYN, and after graft inoculation with PVYO. Biological and serological tests (ELISA) were used for virus detection. The extreme resistance is governed by a single dominant gene, but observed segregations deviated from the expected ratios. Considerable modifying effects were detectable, depending on the potato genotype and virus isolate, for a significant excess of susceptible genotypes was observed in some progenies. Moreover, genotypes with non-parental types of resistance to PVY were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357933
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Variability in the response toPseudomonas solanacearum of transgenic lines of potato carrying a cecropin gene analogue (1995)
    Springer
    Potato research 38 (1995), S. 371-378 
    Details
    ISSN: 1871-4528
    Keywords: bacterial wilt ; resistance ; transformation ; Agrobacterium ; S. tuberosum L. ; transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Transgenic potato plants of cv. Désirée carrying an antibacterial gene, coding for a cecropin lytic peptide analogue, were inoculated with a virulent strain ofPseudomonas solanacearum under controlled conditions. The disease index scored during three repeated infection trials indicated an increased variability in plant response among the transgenic lines which gave either a more susceptible or a more resistant response to the pathogen when compared with untransformed Désirée. Immunity toP. solanacearum was not observed, but it was possible to select a group of transgenic lines that showed resistance levels and disease development curves comparable to the field resistant cv. Cruza 148.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357742
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Correlation between fusaric acid production and virulence of isolates ofFusarium oxysporum that causes potato dry rot in South Africa (1998)
    Springer
    Potato research 41 (1998), S. 289-294 
    Details
    ISSN: 1871-4528
    Keywords: post harvest disease ; potato ; Solanum tuberosum L. ; resistance ; toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Virulence of 12Fusarium oxysporum isolates was determined on three potato cultivars Late Harvest, BP1 and Kimberley Choice. The production of fusaric acid by the 12F. oxysporum isolates was also determined. The aim of the study was to determine whether a correlation exists between fusaric acid production and virulence ofF. oxysporum isolates. Late Harvest was the most tolerant and BP1 the most susceptible cultivar. Virulence ranking, order of theF. oxysporum isolates with the different cultivars corresponded with the mean of value of the ranking order for all three cultivars. A correlation was found between virulence of isolates and fusaric acid production except for Late Harvest. Fusaric acid production may play a major role in the development of dry rot in potato tubers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358198
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Relationship between potato cyst nematodes and their principal host. I. A literature review (1997)
    Springer
    Potato research 40 (1997), S. 317-326 
    Details
    ISSN: 1871-4528
    Keywords: Solanum spp. ; Globodera rostochiensis ; G. pallida ; biology ; pathotypes ; damage ; resistance ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A description of the biology of potato cyst nematodes,Globodera rostochiensis andG. pallida, and symptom development in potato crops is presented. This is followed by an overview of resistance and tolerance research and an explanation of how resistance derived fromSolanum andigena was deployed in integrated control schemes on sandy and sandy peat soils in the north-east of The Netherlands. The frequent use of this resistance source resulted in the predominance of ‘new’ pathotypes ofG. rostochiensis and ofG. pallida. The ability of the cyst nematodes to respond to new types of resistance with new pathotypes makes the search and breeding for resistance a continuing process. The absence of tolerance in exS. vernei (VTn)2 62-33-3 cultivars showed that resistance, if it is to be of use in severely infested fields, must be combined with an appreciable level of tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358013
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Leaf effects of wild species ofSolanum and interspecific hybrids on growth and behaviour of the potato tuber moth,Phthorimaea operculella Zeller (1997)
    Springer
    Potato research 40 (1997), S. 417-430 
    Details
    ISSN: 1871-4528
    Keywords: antibiosis ; antixenosis ; Solanum sparsipilum ; S. pinnatisectum ; S. berthaultii ; glandular trichomes ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four introductions ofSolanum berthaultii wild species and sevenS. tuberosumxS. berthaultii hybrids were evaluated by antibiosis and antixenosis laboratory tests with potato tuber moth. Some morphological and chemical traits related to the trichomes were analysed. Two clones of the wild speciesS. sparsipilum and five ofS. pinnatisectum, characterised by high tuber resistance, were tested for leaf resistance, both by antibiosis and antixenosis assays. The genotypes with high density of trichomes A and with high exudate PPO activity, showed a moderate negative effect on pupal weight and fecundity in the antibiosis tests. Trichomes B exudate showed a strong repulsive effect on oviposition of adult in the antixenosis tests. TheS. pinnatisectum clones revealed a good leaf resistance level, whereas theS. sparsipilum clones showed lower levels of antibiosis and antixenosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358002
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Twenty-five years of international potato research, a retrospective and forward look (1996)
    Springer
    Potato research 39 (1996), S. 395-401 
    Details
    ISSN: 1871-4528
    Keywords: germplasm ; TPS ; resistance ; rapid multiplication ; virus ; collaborative research ; research priorities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary After an initial phase of development and programme establishment the activities of the International Potato Center (CIP) grew rapidly from 1981 to 1985, putting emphasis on germplasm distribution, research on diffused-light storage and TPS, and exploration of sources of resistance. Later, strong emphasis was given to genetic resource utilization and field resistance to late blight. The impact of CIP research showed internal rates of return ranging from 26 to 102 percent. At the end of the 1980s CIP's financial resources were cut severely and restructuring the programme was necessary. Through a careful process of prioritizing, programmes are identified which will contribute most to ensuring the continued increase of potato production in developing countries and to continuing the leading role of CIP in potato research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357945
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Molecular analysis of the genome of transgenic rice (Oryza sativa L.) plants produced via particle bombardment or intact cell electroporation (1998)
    Springer
    Molecular breeding 4 (1998), S. 99-109 
    Details
    ISSN: 1572-9788
    Keywords: transgenic rice ; particle bombardment ; cell electroporation ; RAPD ; AFLP ; AFRP ; RAMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the present work we utilised some of the most discriminative molecular tools, such as RAPD, AFLP, AFRP and RAMP, to analyse the genome of independently derived transgenic plants from three elite Italian cultivars (cv. Lido, Carnaroli and Thaibonnet) and found that two methods for direct gene transfer, namely particle bombardment and intact cell electroporation (the latter being a procedure set up in this work), result in transgenic rice (Oryza sativa L.) plants that exhibit negligible genomic changes. This is in contrast with recently published results showing relevant changes in the DNA of transgenic rice plants generated through protoplasts electroporation and of transgenic poplar plants engineered through Agrobacterium tumefaciens infection. Implications of these findings are discussed in the context of selecting appropriate gene transfer methodologies to produce transgenic plants expressing genes of interest while retaining their genomic integrity and, thus, their superior agronomic and/or industrial traits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009627409668
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 11
    Electronic Resource
    Electronic Resource
    Analyses of quantitative trait loci associated with resistance to shape Sclerotinia sclerotiorum in sunflowers (shape Helianthus annuus L.) using molecular markers (1998)
    Springer
    Molecular breeding 4 (1998), S. 215-226 
    Details
    ISSN: 1572-9788
    Keywords: Helianthus annuus ; QTL ; resistance ; RFLP ; Sclerotinia scerotorum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Restriction fragment length polymorphism and isoenzyme markers were used to investigate quantitative trait loci involved in sunflower resistance to mycelial extension of Sclerotinia sclerotiorum on leaves and capitula. Seed weight, oil content and flowering data were also evaluated. Four quantitative trait loci were demonstrated for leaf resistance and two for capitulum resistance. One of these zones appears involved in resistance to both types of S. sclerotiorum attack while the others appear specific for resistance of one part of the plant. Two quantitative trait loci were detected for seed weight, three for oil content and three for flowering date. Individual quantitative trait loci explained 9% to 48% of the phenotypic variability, confirming the polygenic basis of the quantitative traits studied. Overall, the quantitative trait loci explain 60% of the genetic variation for leaf resistance and 38% for capitulum resistance to S. sclerotiorum. One linkage group is particularly interesting since it includes quantitative trait loci for all the five quantitative traits measured. Hypotheses for linkage versus pleiotropy and consequences of all the results in resistance breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009694626992
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Marker-assisted selection for two rust resistance genes in sunflower (1998)
    Springer
    Molecular breeding 4 (1998), S. 227-234 
    Details
    ISSN: 1572-9788
    Keywords: Helianthus annuus ; PCR ; Puccinia helianthi ; RAPD ; rust resistance ; sunflower
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In this study we report on the identification of molecular markers, OX20600 and OO04950, linked to the geneR Adv in the proprietary inbred line P2. This gene confers resistance to most of the pathotypes of Puccinia helianthi identified in Australia. Analysis indicates these RAPD markers are linked to the resistance locus at 0.0 cM and 11 cM respectively. SCAR markers SCX20600 and SCO04950 derived from these two RAPD markers, and SCT06950 derived from a previously reported RAPD marker linked at 4.5 cM from the R 1 rust resistance gene were developed. SCX20600 and SCO04950 were linked at similar distances from their resistance locus as the RAPD markers. SCTO6950 co-segregated completely with rust resistance. The robustness of the R 1 SCAR marker was demonstrated through the amplification of the marker in a diverse range of sunflower germplasm considered to possess the R 1 gene. The SCAR markers forR Adv were not amplified in the sunflower rust differential set thereby supporting the contention that this is a novel resistance gene. They did amplify in a number of proprietary lines closely related to the line P2. This locus is under further investigation as it will be useful in our attempts to use molecular-assisted breeding to produce durable resistance in sunflower to P. helianthi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009667112088
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Rapid RAPD screening of plant DNA using dot blot hybridization (1996)
    Springer
    Molecular breeding 2 (1996), S. 7-10 
    Details
    ISSN: 1572-9788
    Keywords: RAPD ; dot blot hybridization ; chromosome-specific markers ; marker-assisted selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Scoring of the results of RAPD analysis using gel electrophoresis imposes a constraint on throughput. To circumvent this barrier, dot-blot hybridization was substituted for electrophoresis. Arbitrarily amplified fragments from barley and wheat genomic DNA were labelled and used as probes for the identification of identical fragments in subsequent amplification reactions. None of the twelve fragments used as probes exhibited significant levels of croos-hybridization to other fragments amplified by the same arbitrary primer. The strength of the hybridization signal facilitates more accurate and more sensitive detection of diagnostic fragments than gel electrophoresis. In addition, the defined spatial orientation (microtitre dish format) of the ± results provide an excellent format for automated data collection. The use of dot blot hybridization to analyse PCR products well decrease the cost and time requirements of marker-assisted selection. This technique will also facilitate the rapid application of PCR-based maps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171347
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis (1996)
    Springer
    Molecular breeding 2 (1996), S. 225-238 
    Details
    ISSN: 1572-9788
    Keywords: AFLP ; SSR ; simple sequence repeat polymorphism ; germplasm ; microsatellite ; polymorphism ; RAPD ; RFLP ; soybean ; Glycine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The utility of RFLP (restriction fragment length polymorphism), RAPD (random-amplified polymorphic DNA), AFLP (amplified fragment length polymorphism) and SSR (simple sequence repeat, microsatellite) markers in soybean germplasm analysis was determined by evaluating information content (expected heterozygosity), number of loci simultaneously analyzed per experiment (multiplex ratio) and effectiveness in assessing relationships between accessions. SSR markers have the highest expected heterozygosity (0.60), while AFLP markers have the highest effective multiplex ratio (19). A single parameter, defined as the marker index, which is the product of expected heterozygosity and multiplex ratio, may be used to evaluate overall utility of a marker system. A comparison of genetic similarity matrices revealed that, if the comparison involved both cultivated (Glycine max) and wild soybean (Glycine soja) accessions, estimates based on RFLPs, AFLPs and SSRs are highly correlated, indicating congruence between these assays. However, correlations of RAPD marker data with those obtained using other marker systems were lower. This is because RAPDs produce higher estimates of interspecific similarities. If the comparisons involvedG. max only, then overall correlations between marker systems are significantly lower. WithinG. max, RAPD and AFLP similarity estimates are more closely correlated than those involving other marker systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00564200
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    A first linkage map of Cichorium intybus L. using a one-way pseudo-testcross and PCR-derived markers (1997)
    Springer
    Molecular breeding 3 (1997), S. 415-425 
    Details
    ISSN: 1572-9788
    Keywords: AFLP ; SAMPL ; RAPD ; interspecific hybrid ; molecular map ; chicory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We have used a one-way pseudo-testcross mapping strategy in combination with different types of PCR-based markers (RAPD, AFLP, SAMPL) to construct a first linkage map for variegated chicory (Cichorium intybus L. var. silvestre Biskoff, n=9), a self-incompatible vegetable species. The success of such a strategy depends on the presence of sufficiently high levels of heterozygosity in the individual plant which is being mapped and on the informativeness of the marker system that is used. A total of 371 markers, comprising 16 RAPDs, 72 SAMPLs and 283 AFLPs, were scored in 46 F1 individuals obtained from an interspecific cross between a C. intybus outbred individual and a C. endivia inbred line. Grouping of the markers at a LOD score of 4.0 resulted in 13 linkage groups covering 1330 cM. A framework map covering 1201.4 cM was assembled by using all markers that could be ordered with a LOD greater than 2.0. We estimate the total genome size of chicory to be ca. 1405 cM, thus considerably smaller than that estimated for lettuce (1950 cM). The usefulness of the different marker systems that were applied is analysed in terms of level of heterozygosity and marker index, i.e. number of different genetic loci that may be simultaneously analysed per experiment. Out of the 371 markers, 50 of them showed segregation distortion which is discussed in terms of the hybrid origin of the variegated chicory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009616801404
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    Beet curly top virus DI DNA-mediated resistance is linked to its size (1997)
    Springer
    Molecular breeding 3 (1997), S. 213-217 
    Details
    ISSN: 1572-9788
    Keywords: geminiviruses ; beet curly top virus ; DI DNA ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Beet curly top virus (BCTV) infection is associated with the de novo synthesis of a heterogeneous population of subgenomic viral DNAs. Nicotiana benthamiana plants transformed with a partial repeat of one such subgenomic DNA remained susceptible to infection but produced ameliorated symptoms when agroinoculated with BCTV. Symptom amelioration is associated with the mobilization of subgenomic DNA from the integrated copy. In an attempt to improve the resistance, N. benthamiana has been transformed with a partial repeat of a much smaller subgenomic DNA. However, transgenic plants showed almost no resistance although subgenomic DNA was mobilised from the host genome. To further understand the molecular basis of the interference phenomenon, we compared the ability of BCTV to replicate and accumulate in leaf discs derived from resistant and non-resistant transgenic plants. Both subgenomic DNAs were able to interfere with virus replication but only in case of resistant plants the DI DNA efficiently suppressed viral accumulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009623010404
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Development of coupling-repulsion-phase SCAR markers diagnostic for the sugar beet Rr1 allele conferring resistance to rhizomania (1997)
    Springer
    Molecular breeding 3 (1997), S. 231-238 
    Details
    ISSN: 1572-9788
    Keywords: BNYVV ; BSA ; RAPD ; rhizomania resistance ; SCAR ; sugar beet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In sugar beet genotypes with the ‘Holly’ type of resistance to rhizomania, a disease due to infection of the beet necrotic yellow vein virus (BNYVV), the major gene rrl is responsible for resistance. Twelve RAPD markers linked to rrl were selected by BSA and mapped on linkage group IV using a segregating population previously analysed by the same group. Markers F61050 and N9600 were tightly linked, respectively in coupling and repulsion, to the Rrl allele (recombination values of 1.4 cM for both markers). After sequencing the products amplified by F61050 and N9600, new PCR primers were used to generate the two SCAR markers F6 and N9. The simultaneous use of these markers in a PCR reaction allows the correct fingerprinting of rrl rrl, Rrl rrl and Rrl Rrl sugar beet plants in populations segregating for the ‘Holly’ resistance. In a group of sugar beet elite lines containing the ‘Holly’ type of rhizomania resistance, SCAR F6 is always present whereas the SCAR N9 fragment is absent. Thus, in marker-assisted selection with coupling-repulsion-phase markers, SCAR F6 can be used in combination with N9, or together with any other RAPD marker linked in repulsion to the Rrl allele.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009626214058
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Comparison of PCR-based molecular marker analyses of Musa breeding populations (1999)
    Springer
    Molecular breeding 5 (1999), S. 233-244 
    Details
    ISSN: 1572-9788
    Keywords: Musa ; plantain ; RAPD ; VNTR ; AFLP ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Progress in the breeding of plantain and banana has been restricted by the complex genetic structure and behaviour of cultivated polyploid Musa. Genetic improvement has been hindered due to the large amount of space required for growth and maintenance of plant populations, in addition to the long growth cycle and the low levels of fertility and seed viability characteristic of cultivated genotypes. Molecular marker assisted breeding has the potential to dramatically enhance the pace and efficiency of genetic improvement in Musa. This study was conducted to compare different PCR-based marker systems (RAPD, VNTR and AFLP) for the analysis of breeding populations generated from two diverse Musa breeding schemes. All three assays detected a high level of polymorphism between parental genotypes and within progeny populations. As expected, AFLP assays had by far the highest multiplex ratio while VNTR analysis detected the highest levels of polymorphism. AFLP analysis of a full-sib tetraploid hybrid population confirmed previous reports based on VNTR analysis, of a high frequency of recombination during 2n (3x) gamete formation by a triploid plantain landrace. In addition, both VNTR and RAPD analyses of a full-sib triploid hybrid population suggested a high frequency of homoeologous recombination during n (2x) gamete formation by tetraploid hybrids. In general, there was a poor correlation between estimates of genetic similarity based on different types of marker. The implications of these findings for the molecular breeding of Musa crops are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009649521009
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    Evaluation of AFLP for genetic mapping in Pinus radiata D. Don (1999)
    Springer
    Molecular breeding 5 (1999), S. 275-281 
    Details
    ISSN: 1572-9788
    Keywords: AFLP ; DNA markers ; genetic mapping ; marker-aided selection ; Pinus radiata ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Efficient construction of reasonable density genetic linkage maps is an essential component of QTL detection programmes. The AFLP technique has been used to produce genetic linkage maps in a range of species. We have developed protocols to generate reproducible AFLP profiles in Pinus radiata and have evaluated the inheritance and informativeness of AFLP markers in this important timber species. The large genome size of P. radiata necessitated increased levels of selection at both the pre-amplification and selective amplification steps of the AFLP protocol to generate reproducible AFLP profiles. Once optimised ca. 41.3 scorable AFLP bands were resolvable through denaturing gels, of which 48.4% were polymorphic in a screen of eight unrelated trees. This level of polymorphism is ca. three times higher than with RAPD markers. The total number of bands and the number of polymorphismic bands per PCR were ca. halved when AFLPs were electrophoresed on non-denaturing gels and stained with ethidium bromide. Using the protocols developed, AFLP is an efficient method for generating the DNA markers required for genetic linkage map construction in P. radiata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009612709760
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    Marker-assisted transfer of acylsugar-mediated pest resistance from the wild tomato, Lycopersicon pennellii, to the cultivated tomato, Lycopersicon esculentum (1997)
    Springer
    Molecular breeding 3 (1997), S. 307-317 
    Details
    ISSN: 1572-9788
    Keywords: insect resistance ; marker-assisted selection ; PCR ; quantitative trait loci ; RAPD ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Acylsugars exuded from type IV trichomes mediate the multiple pest resistance found in the wild tomato species, Lycopersicon pennellii. A marker-assisted selection breeding program was used to attempt the transfer of the ability to accumulate acylsugars to cultivated tomato. RFLP and PCR-based markers were used through three backcross generations to select plants containing 5 target regions associated by QTL analysis with acylsugar accumulation. The BC1F1 plant selected possessed all 5 target regions and accumulated acylsugars at a moderate level similar to that of the interspecific F1 control. The BC2F1 and BC3F1 selections contained complementary subsets of the 5 target regions and did not accumulate acylsugars. BC3F1 plants with complementary subsets of the 5 target regions were intermated to produce populations segregating for the 5 target regions. From 1000 BC3F1-intermated plants, three plants were found which accumulated acylsugars at low levels and contained 3 to 5 of the target regions. The recovery of acylsugar accumulation in progeny of the intermated BC3F1 plants supports the involvement of at least some of the 5 target regions in acylsugar biosynthesis. However, since the levels of acylsugars accumulated by these plants were lower than that of the interspecific F1, it is likely that another, as of yet unidentified, region is necessary for accumulation of higher levels of acylsugars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009677412902
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 21
    Electronic Resource
    Electronic Resource
    Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories (1997)
    Springer
    Molecular breeding 3 (1997), S. 381-390 
    Details
    ISSN: 1572-9788
    Keywords: DNA markers ; RAPD ; AFLP ; SSR ; microsatellite ; network ; reproducibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A number of PCR-based techniques can be used to detect polymorphisms in plants. For their wide-scale usage in germplasm characterisation and breeding it is important that these marker technologies can be exchanged between laboratories, which in turn requires that they can be standardised to yield reproducible results, so that direct collation and comparison of the data are possible. This article describes a network experiment involving several European laboratories, in which the reproducibility of three popular molecular marker techniques was examined: random-amplified fragment length polymorphism (RAPD), amplified fragment length polymorphism (AFLP) and sequence-tagged microsatellites (SSR). For each technique, an optimal system was chosen, which had been standardised and routinely used by one laboratory. This system (genetic screening package) was distributed to different participating laboratories in the network and the results obtained compared with those of the original sender. Different experiences were gained in this exchange experiment with the different techniques. RAPDs proved difficult to reproduce. For AFLPs, a single-band difference was observed in one track, whilst SSR alleles were amplified by all laboratories, but small differences in their sizing were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009612517139
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 22
    Electronic Resource
    Electronic Resource
    A major locus for submergence tolerance mapped on rice chromosome 9 (1996)
    Springer
    Molecular breeding 2 (1996), S. 219-224 
    Details
    ISSN: 1572-9788
    Keywords: Oryza sativa L. ; submergence tolerance ; RAPD ; RFLP ; genome mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Submergence stress is a widespread problem in rice-growing environments where drainage is impeded. A few cultivars can tolerate more than 10 days of submergence, but the genes conferring this tolerance have not been identified. We used randon-amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers to map submergence tolerance in 169 F2 plants and the resulting F3 families of a cross between a tolerant indica rice line, IR40931-26, and a susceptible japonica line, PI543851. IR40931-26 inherited strong submergence tolerance from the unimproved cultivar FR13A. Eight-day old F3 seedlings were submerged for 14–16 days in 55-cm deep tanks, and tolerance was scored after 7 days recovery on a scale of 1 (tolerant) to 9 (susceptible). The tolerant and susceptible parents scored 1.5 and 8.4, respectively, and the F3 means ranged from 1.6 to 8.9. Two bulks were formed with DNA from F2 plants corresponding to the nine most tolerant and the nine most susceptible F3 families. Of 624 RAPD primers used to screen the bulks, five produced bands associated with either tolerance or susceptibility. These markers were mapped to a region of chromosome 9 by linkage to RFLP markers. A submergence tolerance quantitative trait locus (QTL), here designatedSub1, was located ca. 4 cM from the RFLP marker C1232 and accounted for 69% of the phenotypic variance for the trait.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00564199
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 23
    Electronic Resource
    Electronic Resource
    RAPD analysis of genetic variation between a group of rose cultivars and selected wild rose species (1996)
    Springer
    Molecular breeding 2 (1996), S. 321-327 
    Details
    ISSN: 1572-9788
    Keywords: RAPD ; rose genetic variability ; molecular marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic variability based on random-amplified polymorphic DNA markers was analysed among 10 cultivated rose varieties and 9 wild species from three different series of the genus Rosa. Using 13 different RAPD primers, 104 polymorphic DNA fragments with a high potential to differentiate rose genotypes could be produced. A dendrogram displaying the relative genetic similarities among the genotypes shows the existence of large genetic diversity among the cultivated roses as compared to the wild species. Furthermore, the main clusters found here are in agreement with known pedigrees and the classical taxonomy. However, the relationships between cultivated roses as inferred by RAPD markers do not correlate with the classical rose classification system. From the present data it is concluded that cultivated roses display a high level of genetic variability despite the fact that single morphological and physiological characters may be less polymorphic within rose groups. This contrasts with the widely accepted opinion of a lack of genetic variability in roses. This is also in accordance with the reported history of rose breeding which makes it highly probable that rose genomes comprise mosaics of different species genomes. As a consequence, it may be possible to utilize the high genetic variability of all genetic traits not under actual selection by breeders for future breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437910
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 24
    Electronic Resource
    Electronic Resource
    Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato (1997)
    Springer
    Molecular breeding 3 (1997), S. 127-136 
    Details
    ISSN: 1572-9788
    Keywords: AFLP ; genetic relationships ; potato ; RAPD ; SSR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The application of AFLPs, RAPDs and SSRs to examine genetic relationships in the primary northwestern European cultivated potato gene pool was investigated. Sixteen potato cultivars were genotyped using five AFLP primer combinations, 14 RAPD primers, and 17 database-derived SSR primer pairs. All three approaches successfully discriminated between the 16 cultivars using a minimum of one assay. Similarity matrices produced for each marker type on the basis of Nei and Li coefficients showed low correlations when compared with different statistical tests. Dendrograms were produced from these data for each marker system. The usefulness of each system was examined in terms of number of loci revealed (effective multiplex ratio, or EMR) and the amount of polymorphism detected (diversity index, or DI). AFLPs had the highest EMR, and SSRs the highest DI. A single parameter, marker index (MI), which is the product of DI and EMR, was used to evaluate the overall utility of each marker system. The use of these PCR-based marker systems in potato improvement and statutory applications is discussed. Abbreviations: PCR, polymerase chain reaction; AFLP, amplified fragment length polymorphism; RAPD, randomly amplified polymorphic DNA; DNA, deoxyribonucleic acid; EMR, effective multiplex ratio; DI, diversity index; MI, marker index; RFLP, restriction fragment length polymorphism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009633005390
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 25
    Electronic Resource
    Electronic Resource
    A molecular marker associated with the H21 Hessian fly resistance gene in wheat (1997)
    Springer
    Molecular breeding 3 (1997), S. 177-181 
    Details
    ISSN: 1572-9788
    Keywords: bulked segregant analysis ; H21 ; near-isogenic lines (NILs) ; RAPD ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Near-isogenic lines in conjunction with bulked segregant analysis were used to identify a DNA marker in wheat (Triticum aestivum L.) associated with the H21 gene conferring resistance to biotype L of Hessian fly [Mayetiola destructor (Say)] larvae. Near-isogenic lines were developed by backcross introgression BC3F3:4 (‘Coker 797’ * 4 / ‘Hamlet’) and differed by the presence or absence of H21 (on 2RL) derived from ‘Chaupon’ rye (Secale cereale L.). Bulked DNA samples were prepared from near-isogenic lines and BC3F2 population individuals segregating for reaction to Hessian fly biotype L and screened for random amplified polymorphic DNA markers using 46 10mer primers. Random-amplified polymorphic DNA markers from resistant and susceptible individuals and parental lines were scored and these data were used to identify a 3 kb DNA fragment that was related to the occurrence of H21. This fragment was amplified from DNA isolated from Hamlet, a near-isogenic line carrying 2RL, and bulked DNA from resistant BC3F2 individuals, but not from the recurrent parent Coker 797 or DNA bulks from susceptible BC3F2 plants. Analysis of 111 BC3F2 segregating individuals and BC3F2:3 segregants confirmed the co-segregation of the 3 kb DNA marker with the H21 resistance gene to Hessian fly. Use of this marker could facilitate more rapid screening of plant populations for Hessian fly resistance and monitoring the introgression of H21.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009606304447
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 26
    Electronic Resource
    Electronic Resource
    Mapping and QTL analysis of horticultural traits in a narrow cross in cucumber (Cucumis sativus L.) using random-amplified polymorphic DNA markers (1997)
    Springer
    Molecular breeding 3 (1997), S. 257-268 
    Details
    ISSN: 1572-9788
    Keywords: determinate ; genetic markers ; multiple lateral branching ; plant architecture ; quantitative trait loci ; sex expression ; cucumber ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An 80-point genetic map [77 random-amplified polymorphic DNAs (RAPD), F (female sex expression), de (determinate), and ll (little leaf)] was constructed from a narrow cross in cucumber using the determinate, gynoecious, standard-sized leaf line G421 and the indeterminate, monoecious, little leaf line H-19. The map defined nine linkage groups and spanned ca. 600 cM with an average distance between markers of 8.4 ± 9.4 cM. The RAPD loci BC-551 and BC-592 were found to flank ll at 3.4 and 12.2 cM, respectively. The locus OP-L18-2 was linked (16 cM) to de, and the F locus was flanked by markers at 44 and 31 cM. One-hundred F3 families were used to identify quantitative trait loci (QTL) for sex expression, main stem length, number of lateral branches, days to anthesis, fruit number and weight, fruit length and diameter, and fruit length: diameter ratio in two replicated test locations (Wisconsin and Georgia). QTL on linkage group B explained major portions (R2 = ca. 2 to 74%) of the variation observed for sex expression, main stem length, lateral branch number, and fruit diameter (LOD = 2.1 to 29.8). Although ca. 62 to 74% of the variation for sex expression was associated with a putative QTL spanning the F locus (OP-AJ-2 to F and F to de), other regions (three) of the genome were important for the determination of sex in the F3 families examined depending upon environment. The number of genomic regions affecting main stem length (five) and number of lateral branches (three) coincided with expectations as determined by calculations of minimum number of genes in previous studies. Evaluation of QTL associated with several fruit number determinants of early, first-harvest yield demonstrating additive genetic variance (i.e., sex expression, main stem length, and number of laterals) suggests that marker-assisted selection may have utility for the development of determinate, multiple lateral branching germplasm suited for once-over mechanical harvesting in this population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009689002015
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 27
    Electronic Resource
    Electronic Resource
    Evidence for loss of ontogenetic resistance of apple leaves againstVenturia inaequalis (1996)
    Springer
    European journal of plant pathology 102 (1996), S. 773-778 
    Details
    ISSN: 1573-8469
    Keywords: apple scab ; environmental factors ; resistance ; Venturia inaequalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The susceptibility of apple leaves toVenturia inaequalis was investigated by assessing disease on individual leaves of seedlings and labelled shoots of orchard trees. Four sets (A-D) of potted seedlings of cv. ‘Golden Delicious’, which had been grown in a glasshouse, each with approximately 30 mature leaves, were exposed to a high-inoculum orchard. Sets A and B were exposed after each other for 47 and 42 days, respectively. As a result of the six and three infection periods during exposure, 94% and 81%, respectively of the seedling shoot tips in set A and set B became infected. However, due to ontogenetic resistance, disease incidence was low in both sets on leaves which were fully expanded at the beginning of exposure. Set C was exposed during both periods (89 days) in which sets A and B were exposed. Not only were all the seedling shoot tips in set C infected, but also — due to the loss of ontogenetic resistance — nearly all of the mature leaves. Ontogenetic resistance was also lost in set D, which was exposed for 57 days at the end of the growing season. The symptoms on fully expanded leaves on plants in sets C and D were typical and similar to those on young leaves. A time course symptom assessment was performed on leaves which had developed early in the season on labelled, field-grown shoots of cv. ‘Golden Delicious’ trees. A steady increase of disease incidence was detected, which could not only have resulted from infections followed by extended incubation periods. In addition to the increase of typical lesions on both leaf surfaces, there was also an abundance of diffuse mycelial development on the lower surfaces of the leaves of the field-grown trees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01877152
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 28
    Electronic Resource
    Electronic Resource
    Molecular records of micro-evolution within the Algerian population of Fusarium oxysporum f. sp. albedinis during its spread to new oases (1997)
    Springer
    European journal of plant pathology 103 (1997), S. 485-490 
    Details
    ISSN: 1573-8469
    Keywords: bayoud disease ; date palm ; population genetics ; RAPD ; RFLP ; VCG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic diversity of the date palm wilt pathogen Fusarium oxysporum f. sp. albedinis in Algeria was assessed using vegetative compatibility, restriction fragment length polymorphism (RFLP) of mitochondrial DNA (mtDNA), and random amplified polymorphic DNA (RAPD). Ninety-eight isolates were collected from the main infested regions, Touat, Gourara and Mzab, and 6 isolates from Morocco were added for comparison. All isolates were vegetatively compatible and belonged to VCG 0170. No variation was detected in the mtDNA of a subset of 73 isolates and the RAPD analysis indicated that they were genetically very closely related. However, some geographic substructuring was apparent, suggesting that local diversification of the pathogen might have occurred. These results provide evidence that the Algerian isolates of F. oxysporum f. sp. albedinis belong to a same clonal lineage and support the hypothesis that they were probably founded by a single virulent clone that originated from the Moroccan oases where the date palm wilt (Bayoud disease) was first detected. Based on similarity of RAPD patterns occurring in different oases, and on historical records of the Bayoud disease in Algeria, spread of the pathogen in the different regions is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008644515046
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 29
    Electronic Resource
    Electronic Resource
    Subdivision and genetic structure of four populations of Venturia inaequalis in Switzerland (1997)
    Springer
    European journal of plant pathology 103 (1997), S. 565-571 
    Details
    ISSN: 1573-8469
    Keywords: apple scab ; PCR-RFLP ; population genetics ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Analyses of four populations of Venturia inaequalis in Switzerland were performed to obtain information about migration and to predict the probable speed of the spread of new pathotypes able to overcome resistance, e.g. Vf-resistance, of new cultivars. Genetic and haplotype diversity was calculated based on allele frequencies of random amplified polymorphic DNA (RAPD) markers and the internal transcribed spacer (ITS)-region of ribosomal DNA, which are regarded to be neutral, and the β-tubulin locus which may be under selection pressure. Within-population diversity was found to be quite similar over all four populations. Normalised haplotype diversity based on RAPD and ITS data was very high with a mean of 0.95. Diversity among populations (GST) was consistent over all neutral loci with a low mean of 0.04, but reached the high value of 0.26 for the selected β-tubulin locus. Low GST based on neutral loci may suggest a high level of gene flow. Considering these results, new pathotypes would be expected soon outside their place of identification. But actual gene flow is easily overestimated because of effects of gene flow in the past. However, naturally occurring gene flow could be increased by human activity. Therefore, it is very difficult to predict durability of the Vf-resitance in Switzerland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008636913211
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 30
    Electronic Resource
    Electronic Resource
    Components of maritime pine susceptibility to twisting rust – A path coefficient analysis (1997)
    Springer
    European journal of plant pathology 103 (1997), S. 653-665 
    Details
    ISSN: 1573-8469
    Keywords: avoidance ; Melampsora pinitorqua ; path coefficient analysis ; Pinus pinaster ; resistance ; rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Data collected in a three-year field experiment on infection and damage by Melampsora pinitorqua on maritime pines showing contrasting growth were analysed by means of path analysis. The severity of infection in 2 year-old pines could be mainly explained by the amount of susceptible tissues (elongating shoots). In contrast, two years later, ‘intrinsic susceptibility’, i.e. expressed after inoculation, was shown to have the most important direct effect on the amount of infection. Intrinsic susceptibility could be satisfactorily assessed by artificial inoculations on excised shoots under controlled conditions. Shoot potassium and phosphorous contents were shown to have marked positive effects on susceptibility. The hypothesis of an age effect on the relative importance of different resistance mechanisms, i.e. avoidance and active resistance, in maritime pine interaction with twisting rust is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008640819188
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 31
    Electronic Resource
    Electronic Resource
    Cacao resistance to Phytophthora: Effect of pathogen species, inoculation depths and pod maturity (1998)
    Springer
    European journal of plant pathology 104 (1998), S. 11-15 
    Details
    ISSN: 1573-8469
    Keywords: black pod ; resistance ; Theobroma cacao
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two species of Phytophthora (P. palmivora and P. capsici) and inoculations at two depths (3 mm and 9 mm) were tested each on 10 clones of Theobroma cacao to determine their effects on pod resistance. Ripe and unripe pods were also assessed to determine the influence of physiological status of the pod on the expression of resistance. The two pathogens tested (P. palmivora and P. capsici) differed significantly in their reactions on pods, with P. palmivora being more aggressive than P. capsici. However, the lack of interaction between clones and pathogen species and the similarity in the ranking of clones based on lesion size suggested that selection for resistant clones can be based on one of the two pathogens, preferably the more aggressive one. Pod reactions differed between inoculation depths (3 mm and 9 mm), and between pod maturity stages (ripe and unripe pods) with relatively larger lesions being recorded at 9 mm depth and on unripe pods as compared to those observed at 3 mm depth and on unripe pods, respectively. The magnitude of increase in lesion sizes, however, varied with genotypes, indicating that inoculation depth and pod maturity stage should be standardized in screening cacao germplasm for resistance to Phytophthora.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008622115731
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 32
    Electronic Resource
    Electronic Resource
    Occurrence in Italy and characteristics of lettuce downy mildew (Bremia lactucae) resistant to phenylamide fungicides (1998)
    Springer
    European journal of plant pathology 104 (1998), S. 449-455 
    Details
    ISSN: 1573-8469
    Keywords: lettuce ; lettuce downy mildew ; Bremia lactucae ; phenylamide fungicides ; virulence factors ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Control of lettuce downy mildew (Bremia lactucae) with phenylamide fungicides has failed in some intensive lettuce-producing areas in Northern Italy since Spring 1993. Before then, these chemicals and particularly metalaxyl, provided the best disease control. The sensitivity of Bremia lactucae isolates collected in such areas to metalaxyl was evaluated in the laboratory. These strains grew and sporulated profusely on lettuce seedlings treated with 100 and 200 ppm of metalaxyl, whereas sensitive control strains were completely inhibited when treated with fungicide concentrations ranging from 0.5–1 ppm. Thus in Italy occurrence of resistance to phenylamide fungicides in Bremia lactucae has also been demonstrated, as in almost all the countries where these chemicals were previously authorised. Subsequently, a demonstration of occurrence of resistance was made and the virulence pattern of several strains (resistant and sensitive to metalaxyl) was characterised using differential NL series containing the 13 DM resistant genes or R factors. The results suggest the occurrence of a new pathotype in Italy different from all the 16 NL Bremia lactucae races studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008696021621
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 33
    Electronic Resource
    Electronic Resource
    Fine Structure of the Early Interaction of Lily Roots with Fusarium oxysporum f.sp. lilii (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 431-443 
    Details
    ISSN: 1573-8469
    Keywords: basal rot ; cell wall degradation ; Lilium ; pathogenesis ; plasmolysis ; resistance ; transfer cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The early interaction of lily roots with the cortical rot pathogen Fusarium oxysporum f.sp. lilii was studied using roots of lily bulblets grown in Hoagland's solution, inoculated with the pathogen, and sampled up to 48 h later. Conidia produced germ tubes within 6 h, which extended towards and into the mucilage covering the root elongation zone, and along and into the anticlinal grooves and middle lamellae of epidermal cells. By 24–48 h, infecting hyphae had reached the periclinal walls and intercellular spaces between the epidermis and the outermost cells of the cortex. Penetration of intercellularly growing hyphae directly across host cell walls was not observed; invasion of the cell lumen only occurred by gradual infringing of hyphae upon successive primary wall layers. Non-cellulosic wall appositions rich in vesicles and covered by a cellulosic protective-like layer were formed in response to approaching hyphae in resistant cv.Connecticut King, but rarely in susceptible cv. Esther which seemed more susceptible to plasmolysis and rot. Finger-like projections of the appositions into the host cell cytoplasm likely represent early stages of transfer cell formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008749514517
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 34
    Electronic Resource
    Electronic Resource
    Accumulation of phytoalexins in leaves of plane tree (Platanus spp.) expressing susceptibility or resistance toCeratocystis fimbriata f. sp.platani (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 503-509 
    Details
    ISSN: 1573-8469
    Keywords: Ceratocystis fimbriata ; phytoalexins ; Platanus spp. ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Inoculation of leaves of resistantPlatanus occidentalis and susceptiblePlatanus acerifolia leaves withCeratocystis fimbriata f. sp.platani, the canker stain disease agent, induced foliar necrosis and biosynthesis of two phytoalexins, scopoletin and umbelliferone. Foliar symptoms keep localized and accumulation of coumarin phytoalexins was rapid for incompatible interactions. Necrosis spread widely and accumulation of these phenolic compounds was much later and lower for compatible interactions. The differential response could be used in a genetic improvement program for resistance against canker stain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01874474
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 35
    Electronic Resource
    Electronic Resource
    Differential interactions ofPhytophthora capsici isolates with pepper genotypes at various plant growth stages (1996)
    Springer
    European journal of plant pathology 102 (1996), S. 311-316 
    Details
    ISSN: 1573-8469
    Keywords: Capsicum annuum ; C. frutescens ; resistance ; Phytaphthora capsici ; host-pathogen interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pepper cultivars from diverse geographic origins were evaluated for resistance to different isolates ofPhytophthora capsici under controlled environmental conditions. All accessions tested were susceptible at the four-leaf stage to the six isolates ofP. capsici. Inoculation at the eight-leaf stage resulted in significantly different interactions among the accessions andP. capsici isolates. The Korean and U.S. cultivars tested were highly susceptible to the isolates ofP. capsici at this stage. In contrast, PI 201234 and PI 201238 had a differential interaction with someP. capsici isolates. At the twelve-leaf stage, Phytophthora blight developed slowly in the Korean and U.S. cultivars that were highly susceptible at the eight-leaf stage. Furthermore, the accessions from the Asian Vegetable Research and Development Center (AVRDC) became highly resistant toP. capsici at this stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01878125
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 36
    Electronic Resource
    Electronic Resource
    Morphological and molecular characterization of Colletotrichum spp. from citrus orchards affected by postbloom fruit drop in Brazil (1997)
    Springer
    European journal of plant pathology 103 (1997), S. 323-329 
    Details
    ISSN: 1573-8469
    Keywords: Colletotrichum acutatum ; citrus postbloom fruit drop disease ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Brazilian isolates of Colletotrichum spp. from citrus orchards affected by postbloom fruit drop were examined for colony colour, mycelial growth, benomyl-resistance, pathogenicity, and genetic variability by random amplified polymorphic DNA (RAPD) analysis. All isolates were obtained from flowers and persistent calyxes from different citrus hosts from Sao Paulo, Brazil. DNA polymorphisms detected after amplification with random 10-mer primers were used to classify the isolates into two groups. Group I isolates grew rapidly on potato-dextrose agar (PDA) and were sensitive to benomyl, and group II isolates grew slowly on PDA and were benomyl-resistant. Colletotrichum acutatum was analyzed by RAPD and had high genetic similarity with group II isolates of Colletotrichum from citrus. Probably, the group I is C. gloeosporioides and group II is C. acutatum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008627302706
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 37
    Electronic Resource
    Electronic Resource
    Fungal polygalacturonase activity reflects susceptibility of carnation cultivars to fusarium wilt (1997)
    Springer
    European journal of plant pathology 103 (1997), S. 15-23 
    Details
    ISSN: 1573-8469
    Keywords: breeding ; Dianthus caryophullus ; Fusarium oxysporum ; pectic enzymes ; phytoalexins ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Carnation cultivars with different levels of partial resistance were inoculated with race 2 of Fusarium oxysporum f.sp. dianthi and monitored for accumulation of host phytoalexins, fungal escape from compartmentalization, production of fungal pectin-degrading enzymes and development of external disease symptoms. Accumulation of phytoalexins, assessed after 10 days in the first 5 cm above the inoculation site, was weakly (methoxydianthramide S) or not (hydroxydianthalexin B) correlated with resistance levels after 12 weeks. Fungal escape from compartmentalization, assessed after 3 weeks as percentages colonized plants at 8 cm above the inoculation site, was highly correlated with expression of susceptibility after 12 weeks. Polygalacturonase (PG) activity, assessed after 4 weeks in the first 5 cm above the inoculation site, was highly correlated to final disease development. Linear increases in disease severity were accompanied by quadratic increases in PG activity. In contrast to water-treated plants, that lacked any PG activity, inoculated plants contained two main groups of fungal PGs, the dominant forms of which had estimated pI values of 7.0 and minimally 9.5, respectively. Compared to those of the first group, enzymes of the second group were produced only in trace amounts in liquid media containing pectin or polygalacturonate as sole source of carbon. On these media, the fungus also produced a pectin methyl esterase (PME) with an estimated pI of 9.3. Besides PMEs of host origin, inoculated plants of susceptible cultivars contained the fungal PME while no more than traces were found in resistant ones. Assessment of phytoalexin production by the host during defense responses cannot replace monitoring of external symptoms as a resistance test. Assessment of fungal growth, whether by reisolations above the compartmentalization area or by measurement of PG activity, provides a both rapid and reliable prediction of disease development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008616008804
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 38
    Electronic Resource
    Electronic Resource
    Molecular marker analysis of European Setosphaeria turcica populations (1998)
    Springer
    European journal of plant pathology 104 (1998), S. 611-617 
    Details
    ISSN: 1573-8469
    Keywords: Exserohilum turcicum ; Johnson grass ; maize ; northern corn leaf blight ; population genetic structure ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Setosphaeria turcica is the causal agent of northern corn leaf blight, a foliar maize disease of worldwide economic importance. In Europe, its severity increases. To investigate the pathogen's population-genetic structure in central Europe, a total of 80 isolates was sampled in Germany, Switzerland, France, Austria, and Hungary and investigated with 52 random amplified polymorphic DNA (RAPD) markers. The mating type of the isolates was determined in testcrosses. Among the 73 isolates from maize there were 26 different RAPD haplotypes. All isolates with identical haplotype are considered clonemates. The haplotype shared by most members was represented by 22 isolates from Germany, Switzerland, and France, indicating high fitness and substantial migration. Only a single clone had members in both southeastern Austria and southwestern Switzerland, suggesting that the Alps constitute a major barrier for this pathogen. Several haplotypes differed by only one or two RAPD bands from the predominant haplotype and may have arisen by mutation. Few other clonal lineages were detected. The evolution of some haplotypes could not be explained by mutation alone. Sexual recombination may rarely occur. In population samples from Germany, Switzerland, and France, mating type MAT2 was predominating, while most isolates from Austria and Hungary had MAT1. Seven isolates from Johnson grass (Sorghum halepense), an alternative host of S. turcica, were clonemates and very different in RAPD haplotypes from all isolates collected from maize.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008641920356
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 39
    Electronic Resource
    Electronic Resource
    Effects of Temperature on Infection in Capsicum sp. and Nicotiana benthamiana by Impatiens Necrotic Spot Tospovirus (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 509-512 
    Details
    ISSN: 1573-8469
    Keywords: tospovirus ; resistance ; Capsicum sp. ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Infection by two isolates of impatiens necrotic spot tospovirus (INSV) under temperature regimes of 25/18°C (day/night) or 33°C (continuous) was studied in Capsicum annuum (systemically susceptible to tomato spotted wilt tospovirus, TSWV), C. chinense PI 152225 and PI 159236 (reacting hypersensitively to TSWV) and Nicotiana benthamiana (systemically susceptible to both tospoviruses). At 25/18°C infection was systemic in all hosts tested. At 33°C infection in N. benthamiana was systemic whereas in C. annuum and C. chinense it was restricted to the inoculated leaves. The result differed from that reported for TSWV, where high temperature made plants more susceptible, or caused no difference. Exchanging temperature regimes 6 h to 4 days after inoculation did not affect the final results one month later, with plants being only locally infected at 33°C continuous regime, or systemically infected at 25/18°C alternate regime. The two INSV isolates were biologically and serologically stable for 5 passages in N. benthamiana held continuously at 33°C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008742516820
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 40
    Electronic Resource
    Electronic Resource
    A Laboratory Assay for Phytophthora infestans Resistance in Various Solanum Species Reflects the Field Situation (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 241-250 
    Details
    ISSN: 1573-8469
    Keywords: Phytophthora infestans ; potato ; wild Solanum spp. ; resistance ; detached leaves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Physiological and molecular research on resistance responses of Solanum tuberosum cultivars and partially resistant Solanum species to Phytophthora infestans requires a reliable resistance test that can be used in the laboratory. Laboratory tests performed on detached leaves and intact plants were compared with field tests for similarity of late blight reactions. Detached leaves from field-grown plants were as resistant as detached leaves from climate chamber-grown plants when challenged with P. infestans. However, detached leaves incubated in covered trays at high relative humidity were more susceptible than detached leaves kept in open trays or leaves on intact plants. The incubation conditions of detached leaves in covered trays rather than detachment itself appeared to affect the resistance expression. Detached leaves of some wild Solanum genotypes became partially infected, whereas intact plants were completely resistant when inoculated. Inoculation of leaves on intact plants, however, resulted in lower infection efficiencies. These limitations should be taken into account when choosing the appropriate inoculation method for specific purposes. For resistance screening, laboratory tests proved to be a good alternative for field tests. The ranking of resistance levels for twenty plant genotypes was similar under laboratory and field conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008710700363
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 41
    Electronic Resource
    Electronic Resource
    The effect of physiological age of potato tubers on female production of potato cyst nematodes (Globodera pallida Pa 2/3) (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 51-56 
    Details
    ISSN: 1573-8469
    Keywords: resistance ; potato breeding ; roots canister test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fourteen cultivars generally regarded as uniformly susceptible toGlobodera pallida Pa 2/3 were assessed in plastic closed containers for degree of susceptibility and also for root and sprouting characteristics. The material was assessed by scoring degree of sprouting before planting and number of cysts and root growth at the end of experiment at intervals from the time of initial dormancy break through to prolific sprouting. Using covariate analyses, it was found that the degree of root development, particularly in the earlier assessments, significantly influenced the degree of cyst development and so the perceived levels of susceptibility/resistance. Having taken account of root growth characters, significant differences between the cultivars with regard to nematode multiplication were still evident. The implications of the results are discussed in relation to potato breeding and nematological studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01876093
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 42
    Electronic Resource
    Electronic Resource
    Gene and Genotypic Diversity of Phytophthora cinnamomi in South Africa and Australia Revealed by DNA Polymorphisms (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 667-680 
    Details
    ISSN: 1573-8469
    Keywords: asexual reproduction ; mating types ; oomycetes ; origin ; RAPD ; RFLP ; population genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Phytophthora cinnamomi isolates from South Africa and Australia were compared to assess genetic differentiation between the two populations. These two populations were analysed for levels of phenotypic diversity using random amplified polymorphic DNAs (RAPDs) and gene and genotypic diversity using restriction fragment length polymorphisms (RFLPs). Sixteen RAPD markers from four decanucleotide Operon primers and 34 RFLP alleles from 15 putative loci were used. A few isolates from Papua New Guinea known to posses alleles different from Australian isolates were also included for comparative purposes. South African and Australian P. cinnamomi populations were almost identical with an extremely low level of genetic distance between them (Dm=0.003). Common features for the two populations include shared alleles, low levels of phenotypic/genotypic diversity, high clonality, and low observed and expected levels of heterozygosity. Furthermore, relatively high levels of genetic differentiation between mating type populations (Dm South Africa=0.020 and Dm Australia=0.025 respectively), negative fixation indices, and significant deviations from Hardy–Weinberg equilibrium, all provided evidence for the lack of frequent sexual reproduction in both populations. The data strongly suggest that both the South African and Australian P. cinnamomi populations are introduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008755532135
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 43
    Electronic Resource
    Electronic Resource
    Diversity of Rhizobia isolated from various Hedysarum species (1996)
    Springer
    Plant and soil 186 (1996), S. 21-28 
    Details
    ISSN: 1573-5036
    Keywords: Hedysarum spp. ; plasmid profile ; RAPD ; Rhizobium ; serogroup
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cultural and physiological properties, serology, plasmid profiles and infective traits were determined for 23 strains of rhizobia isolated from various Hedysarum species: H. coronarium (common name: sulla) (16), H. carnosum (1), H. alpinum (3), H. mackenzii (2) and H. pallens (1) from Portugal, Spain, Tunisia, Alaska and Israel. Strains isolated from H. alpinum, H. mackenzii and H. pallens have slow growth rates on yeast-extract mannitol medium and were unable to nodulate H. coronarium plants, whereas the latter were effectively nodulated by all sixteen fast growing strains from sulla. Regardless of the country of origin all H. coronarium strains fell into one serogroup and were not serologically related with strains of other Hedysarum species. The RAPD (random amplified polymorphic DNA) fingerprinting method which was carried out on five H. coronarium and three H. alpinum strains allowed distinction to be made among serologically related rhizobia. No particular plasmid profile pattern was observed in relation to the host or geographical origin of the strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035051
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 44
    Electronic Resource
    Electronic Resource
    Bunts and smuts of wheat in North Africa and the Near East (1998)
    Springer
    Euphytica 100 (1998), S. 45-50 
    Details
    ISSN: 1573-5060
    Keywords: incidence ; Karnal bunt ; occurrence ; resistance ; variability ; wild relatives ; progenitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bunts [common bunt (Tilletia laevis and T. tritici) and dwarf bunt (T. controversa)] and smuts [loose smut (Ustilago tritici) and flag smut (Urocystis agropyri)] of wheat are important cereal diseases in most countries of north Africa and the Near East. There are no peculiarities in the occurrence of bunts and smuts, except for dwarf bunt, which is limited to high-altitude areas of Turkey, Iran and Iraq. Host adaptation, rather than topography, determines the distribution of the common bunt pathogens: T. laevis predominates in bread wheat, whereas T. tritici attacks both bread and durum wheat non-preferentially. Incidence of bunt- and smut-affected wheat heads is generally low, however the frequency of their occurrence in fields is high, indicating their potential threat. Some bunts and smuts occur on hosts other than wheat, for example, flag smut on Aegilops crassa, loose smut on Ae. geniculata and rye, and dwarf bunt on Hordeum and Aegilops spp. Genetic variability of the pathogens causing common bunt and loose smut was investigated. Chemical seed treatment is the most widely used control for bunts and smuts. Organic nutrients as seed treatments to control common bunt of wheat show considerable promise. Sources of resistance to loose smut, and common and dwarf bunts of wheat, are available in wheat and its wild relatives. Triticum boeoticum, T. dicoccoides, and Aegilops species represent excellent sources of resistance to common bunt. There are three major sources of resistance in durum wheats, Senatore Cappelli and Haurani, Jenneh Khetifa, and Mindum. Common bunt resistance genes Bt5, Bt6, Bt8, Bt9, Bt10, and Bt11, and several undescribed resistances remain effective in the screening field at ICARDA, Syria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018343603827
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 45
    Electronic Resource
    Electronic Resource
    Genetic variation of sweet potatoes (Ipomoea batatas L.) cultivated in Chile determined by RAPDs (1998)
    Springer
    Euphytica 101 (1998), S. 193-198 
    Details
    ISSN: 1573-5060
    Keywords: sweet potato ; Ipomoea batatas ; RAPD ; genetic variation ; Chile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract RAPD (randomly amplified polymorphic DNA) technology was applied to analyze the genetic variability of sweet potato germplasm existing in Chile and elsewhere. Analysis of 28 cultivars from all over the world showed polymorphic bands with all 18 primers tested. A total of 124 RAPD bands were scored with an average of 6.9 polymorphic bands per primer. These results confirm that sweet potato exhibits high genetic variation. Two groups were distinguished: one containing Peruvian cultivars, and another containing cultivars from the rest of the world. Analysis of 14 accessions from Central Chile and one from Northern Chile showed polymorphic bands with 24 of 26 primers tested, but almost all of the 140 polymorphic bands merely showed the distinctness of the Northern accession. The almost complete uniformity of the other 14 accessions shows that sweet potato germplasm collected in Central Chile has very little genetic variability and may be derived from a single cultivar. Based on these results and on historical records, some hypotheses are proposed to explain the origin of sweet potatoes cultivated in Chile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018301009296
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 46
    Electronic Resource
    Electronic Resource
    Molecular characterization and genetic relatedness among walnut (Juglans regia L.) genotypes based on RAPD markers (1998)
    Springer
    Euphytica 101 (1998), S. 199-206 
    Details
    ISSN: 1573-5060
    Keywords: cultivar identification ; fingerprinting ; genetic similarity ; Juglans regia ; RAPD ; walnut
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The potential use of the Randomly Amplified Polymorphic DNA (RAPD) technique for characterization and assessment of genetic relationships was investigated in nineteen walnut (Juglans regia L.) genotypes used as parents or released as cultivars from the breeding program of the University of California at Davis. Most of the 72 decamer primers used yielded scorable amplification patterns based on discernable bands. The results obtained produced a unique fingerprint for each of the walnut genotypes studied. Cluster analysis separated the 19 walnut genotypes into two main groups whose differences were related to their pedigree. Genotypes sharing common parents tend to group together and with at least one of the parents. Thus, RAPD markers can detect enough polymorphism to differentiate among walnut genotypes, even among closely related genotypes, and the genetic similarity based on RAPDs appears to reflect the known pedigree information. RAPD technology can be useful in current walnut breeding programs, allowing the identification of new cultivars as well as the assessment of the genetic similarity among genotypes which will help in selecting the best parents to obtain new genetic combinations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018390120142
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 47
    Electronic Resource
    Electronic Resource
    Genetic relationships of avocado (Persea americana Mill.) using RAPD markers (1998)
    Springer
    Euphytica 101 (1998), S. 249-255 
    Details
    ISSN: 1573-5060
    Keywords: avocado ; classification ; ecological races ; Persea americana ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract RAPD (randomly amplified polymorphic DNA) analysis was carried out on 16 accessions representing the three ecological races of avocado (Persea americana Mill.), and one accession of P. schiedeana Nees. Twenty two preselected primers produced 133 polymorphic DNA fragments in the RAPD assay of the avocado accessions. One primer was identified which could differentiate each of the avocado accessions. Potentially race-specific markers for each of the Mexican, Guatemalan, and West Indian races, have been detected. A Jaccard's similarity coefficient matrix was generated and a dendrogram constructed using UPGMA (unweighted pair-group method of arithmetic averages) cluster analysis. Percentage similarity between avocado accessions ranged from 46% to 85%. The lowest similarity (between 22% and 29%) was revealed between P. schiedeana and any P. americana accession. Average similarity within races of avocado was 75% for the Mexican race, 71% for the West Indian race and 73% for the Guatemalan race. Average similarity between races ranged from 53% to 58%. The dendrogram identified three groups, representing the races of avocado. These results are in concordance with the present classification of avocado into three subspecies (varieties) of P. americana, namely drymifolia, americana, and guatemalensis, corresponding to the Mexican, West Indian and Guatemalan races, respectively, and confirm the separate species status of P. schiedeana. We conclude that RAPD markers may be useful for the classification of avocado and for the assessment of genetic diversity of avocado germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018321928400
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 48
    Electronic Resource
    Electronic Resource
    Efficacy of bulked DNA samples for RAPD DNA fingerprinting of genetically complex Brassica napus cultivars (1998)
    Springer
    Euphytica 102 (1998), S. 65-70 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; RAPD ; bulked DNA ; DNA fingerprinting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Since DNA-based markers are unaffected by environmental or physiological factors, they have potential utility in the description of plant cultivars required for award of proprietary rights (i.e. Plant Breeders' Rights). The high discriminating power of this class of markers, however, can also make demonstration of uniformity and stability of such a marker within a cultivar difficult, especially for genetically-complex cultivars. This report examines the usefulness of bulking equal quantities of DNA from 14 to 20 individuals of a cultivar to identification of RAPD DNA markers that distinguish between Brassica napus cultivars of varying genetic complexity. For the four cultivars assessed (Quantum, OAC Springfield, Innovator and AC Excel), it is shown that consistent presence/absence scores are obtained from bulked DNA samples for three different RAPD markers despite a significant degree of variation among samples from individuals. Use of bulked DNA samples thus may enable identification of a distinguishing profile of RAPD markers whose presence/absence is uniform and stable even in complex cultivars. Nevertheless, RAPD markers remain limited in that they are not strictly quantitative in nature. This limitation is discussed with respect to cultivar description for plant breeders' rights applications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018378304701
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 49
    Electronic Resource
    Electronic Resource
    The expression of resistance of subterranean clover cultivars to races of Phytophthora clandestina (1998)
    Springer
    Euphytica 102 (1998), S. 71-79 
    Details
    ISSN: 1573-5060
    Keywords: Phytophthora clandestina ; resistance ; root rot disease ; subterranean clover ; Trifolium subterraneum ; virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Infection and sporulation of races of Phytophthora clandestina in susceptible and resistant cultivars of subterranean clover and the effect of the races on growth of the cultivars in pots were studied using a total of five races of the pathogen and eight host cultivars. The pathogen caused severe disease where races and cultivars were compatible, although a significant reduction in root and shoot dry weight occurred only on Woogenellup inoculated with race 2. There was little increase in disease severity after 1 wk from the time of inoculation. Sporangia and oospores were formed at high frequency in tap and lateral roots in all compatible combinations, except in Woogenellup in which sporangia were produced inconsistently. The pathogen caused negligible disease and produced very few sporangia in incompatible combinations. Plant age had a strong effect on disease development. Disease severity caused by virulent races on cultivars Gosse, Trikkala, Meteora and Woogenellup was less on older than on younger plants. A further experiment using aggressive isolates of races 0, 1, 2, 3 and 4 revealed that cultivar Gosse showed no reduction in root and shoot weight, despite it being susceptible to races 1, 3 and 4. This provided evidence of the existence of race-non-specific or horizontal resistance to P. clandestina in cultivar Gosse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018330421539
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 50
    Electronic Resource
    Electronic Resource
    In vitro tolerance and resistance to burrowing nematode, Radopholus similis, in Anthurium species (1998)
    Springer
    Euphytica 103 (1998), S. 23-28 
    Details
    ISSN: 1573-5060
    Keywords: Anthurium ; Radopholus similis ; resistance ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Five Anthurium species closely related to two of the cultivated ornamentals, A. andraeanum Lind. ex André and A. scherzerianum Schott, and one species of breeding interest, were screened in vitro for tolerance and resistance to the nematode Radopholus similis Cobb, 1893. Adjustment of the tolerance measurement to the initial and uninoculated treatment measurement improved the screening method. Use of a nematode strain with a relatively slow reproduction rate enabled concurrent screening for tolerance and resistance to R. similis in Anthurium. Based on a lower relative symptom index, A. pittieri Engl., A. ravenii Croat and Baker, A. antioquiense Engl. and A. aripoense N. E. Br. reduce nematode damage as compared to the reference standard, ‘Midori’. However, A. antioquiense and A. aripoense had higher root damage than ‘Midori’. Lower nematode damage in A. pittieri and A. ravenii is positively correlated with greater plant vigor or to fewer target roots for nematode infection. A. ravenii was among the most resistant species as measured by nematode reproduction. This is followed by A. aripoense and A. pittieri. Thus, strong plant vigor, fewer target roots for nematode infection, and/or lower nematode reproduction (higher nematode resistance) resulted in lower nematode damage in A. pittieri and A. ravenii. Combining the nematode damage and nematode reproduction results, A. pittieri and A. ravenii were identified as more tolerant than the reference standard ‘Midori’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018384019938
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 51
    Electronic Resource
    Electronic Resource
    Genetic diversity within spelta and macha wheats based on RAPD analysis (1998)
    Springer
    Euphytica 104 (1998), S. 181-189 
    Details
    ISSN: 1573-5060
    Keywords: Genetic diversity ; macha wheat ; spelta wheat ; RAPD ; accession duplication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic diversity in a crop species is basic to improvement of the species and can be estimated at the molecular level. The objective of this study was to estimate genetic diversity within and between spelta and macha wheats. Random amplified polymorphic DNA (RAPD) analysis was conducted on 69 spelta and 32 macha wheat accessions. The classification of spelta and macha accessions, based on Jaccard genetic similarity coefficients for RAPD markers, was consistent with their geographic origin. The results indicated that the germplasm of macha wheat was more diverse than that of spelta wheat. In the dendrogram of macha wheat, four spelta-like accessions grouped together, separate from the remaining macha accessions, suggesting that these accessions were misclassified. In addition, accessions with identical RAPD patterns were found, indicating that these accessions were probably duplicated. Thus RAPD analysis can be used to estimate genetic diversity and identify duplicate accessions in wheat germplasm collections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018628102650
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 52
    Electronic Resource
    Electronic Resource
    Detection of molecular markers associated with linolenic and erucic acid levels in spring rapeseed (Brassica napur L.) (1999)
    Springer
    Euphytica 105 (1999), S. 173-181 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napur ; doubled haploids ; RAPD ; linolenic acid ; erucic acid ; marker assisted selection ; rapeseed breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Undesirable characteristic of rapeseed oil is a relatively high level of linolenic acid (18:3), which is easily oxidized leading to rancidity and a shortened shelf life of the oil. Previous attempts to reduce linolenic acid levels in rapeseed oil through breeding have been impaired by complex genetics and strong environmental sensitivity of this trait. Therefore, our objective was to develop molecular markers for low linolenic acid that could facilitate the breeding of low linolenic rapeseed. Bulked segregant analysis was employed to identify two RAPD markers associated with 18:3 in a doubled haploid population segregating for linolenic and erucic acid levels. Based on analysis of individual DH lines, the markers RM350 and RM574, representing two independent loci, accounted for a total of 39% of the genetic variability in this population. This marker RM350 alone accounted for 25% genetic variation for this trait with no evidence of recombination. Significant interlocus interaction found between the markers RM350 and RM574 suggested that epistasis was involved in the genetic control of 18:3 level in this population. Another marker designated as RM322, which was independent of the other two, was found significantly associated with the erucic acid level and oil content. RAPD markers identified in this study should be a useful tool for the early detection of low linolenic, or low or high erucic acid genotypes in rapeseed breeding programs based on doubled haploids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003494217074
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 53
    Electronic Resource
    Electronic Resource
    Identification of RAPD markers linked to fusarium crown and root rot resistance (Frl) in tomato (1999)
    Springer
    Euphytica 105 (1999), S. 205-210 
    Details
    ISSN: 1573-5060
    Keywords: crown rot ; fusarium crown and root rot ; genetic linkage ; Lycopersicon peruvianum ; molecular markers ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In tomato ( Lycopersicon esculentum Mill.) a single dominant gene ( Frl) on chromosome 9 confers resistance to fusarium crown and root rot (crown rot) incited by Fusarium oxysporum f. sp. radicis-lycopersici. To identify randomly amplified polymorphic DNA (RAPD) markers linked to Frl, crown rot susceptible and resistant tomato lines were screened for polymorphisms using 1000 random 10-mer primers and three reliable RAPD markers were found linked to Frl (UBC #'s 116, 194, and 655). A codominant polymorphic PCR marker of TG101, a restriction fragment length polymorphic (RFLP) marker linked to Frl, was developed to facilitate the linkage studies. Using TG101 and the four RAPD markers, on a Frl segregating backcross population of 950 plants indicated that all belong to the same linkage group. The polymorphic allele order was found to be TG101 – 655 – 116 – 194 – Frl. UBC 194 was found to be 5.1 cM from Frl in this population. Furthermore, it was the only marker found in the resistant genotypes ‘Mocis’ and Fla 7226, whereas resistant genotypes ‘Momor’, Ohio 89-1, and Fla 7464 all had UBC 194 and UBC #'s 116, 194, and 655.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003497719705
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 54
    Electronic Resource
    Electronic Resource
    Identification of a RAPD marker linked to a brown planthopper resistance gene in rice (1999)
    Springer
    Euphytica 107 (1999), S. 23-28 
    Details
    ISSN: 1573-5060
    Keywords: Bph-1 ; linkage analysis ; mapping ; RAPD ; RFLP ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We report the tagging of a brown planthopper (BPH) resistance gene (Bph–1) in rice using RAPD and RFLP markers. The Korean rice variety ‘Gayabyeo’ has dominant duplicate genes including Bph–1 conferring resistance to biotype 1 of BPH. Bulked segregant RAPD analysis was employed for rapid identification of DNA markers linked to resistance genes. For tagging these two genes, an F2F3 population from a ‘Gayabyeo’ × ‘Nagdongbyeo’ cross was developed and evaluated for BPH resistance. Three bulked DNAs from two groups of homozygous BPH resistant (each for Bph–1 and the other unknown gene) and homozygous susceptible F2 plants were analyzed by RAPD using 140 random oligomers. One primer, OPD–7 yielded a 700-bp fragment that was present in Gayabyeo and resistant F2 plants (homozygous for Bph-1 locus) but absent in Nagdongbyeo and susceptible F2 plants. Cosegregation of this marker with Bph-1 was verified using an F2 population segregating for Bph-1. Chromosomal regions surrounding the Bph-1 were examined with additional RFLP and microsatellite markers on chromosome 12 to define the location of the RAPD marker and Bph-1. Use of this RAPD marker could facilitate early selection of resistant lines for BPH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003506830735
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 55
    Electronic Resource
    Electronic Resource
    Search for molecular markers linked to Liriomyza trifolii resistance in tomato (1999)
    Springer
    Euphytica 109 (1999), S. 149-156 
    Details
    ISSN: 1573-5060
    Keywords: BSA ; Liriomyza trifolii ; Lycopersicon ; molecular markers ; resistance ; leaf miner
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Resistance to many arthropods, including Liriomyza species, is known to be present in accessions of Lycopersicon hirsutum (f. typicum or f. glabratum). From the cross L. esculentum cv. Moneymaker and L. hirsutum f. glabratum G1561 100 F2 plants were screened in a no-choice test for resistance to Liriomyza trifolii. The Bulked Segregant Analysis approach was used to find Random Amplified Polymorphic DNA markers linked to resistance. Two markers were located on chromosome 2. Restriction Fragment Length Polymorphisms constructed a more detailed genetic linkage map for part of chromosome 2. Kruskal-Wallis analysis showed that this chromosome harbored a Quantitative Trait Locus (QTL) for number of pupae, number of mines and damage. At least one major QTL is essential for resistance and this QTL is located on chromosome 2 nearby the location of the tomato probe TG451.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003728302493
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 56
    Electronic Resource
    Electronic Resource
    Reaction of tritordeum to Fusarium culmorum and Septoria nodorum (1996)
    Springer
    Euphytica 88 (1996), S. 165-174 
    Details
    ISSN: 1573-5060
    Keywords: wheat ; tritordeum ; Hordeum chilense ; Fusarium culmorum ; Septoria nodorum ; scab ; deoxynivalenol ; ergosterol ; resistance ; Triticum spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae. It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum. Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense, followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023887
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 57
    Electronic Resource
    Electronic Resource
    The use of RAPD technique for the identification and classification of Pisum sativum L. genotypes (1996)
    Springer
    Euphytica 89 (1996), S. 229-234 
    Details
    ISSN: 1573-5060
    Keywords: Cultivar identification ; genetic relatedness ; Pisum sativum ; RAPD ; pea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genomic DNAs of 42 Pisum sativum genotypes, representing four wild and cultivated subspecies were used as templates in RAPD reactions. Amplification with eight decamer primers generated 149 polymorphic products. Genetic similarities of RAPD profiles were estimated via a coefficient of Jaccard and then the data were processed by cluster analysis (UPGMA). Each genotype was clearly identified and separated from the others. Our results show that RAPD technology is a rapid, precise and sensitive technique for identification of pea genotypes. However, the phylogenetic relationships within the Pisum sativum, which we tested by bootstrap analysis (Wagner parsimony), must be interpreted with caution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034610
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 58
    Electronic Resource
    Electronic Resource
    Screening of Kabuli chickpea germplasm for resistance to Fusarium wilt (1997)
    Springer
    Euphytica 96 (1997), S. 273-279 
    Details
    ISSN: 1573-5060
    Keywords: Cicer arietinum ; chickpea ; Fusarium oxysporum f.sp. ciceri ; wilt ; screening ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A total of 1915 Kabuli chickpea lines were screened in a wilt sick plot containing Fusarium oxysporum f.sp. ciceri race 0 at Béja, Tunisia. Complete resistance was found in 110 lines and this result was confirmed by a laboratory screening method. Principal components analysis showed that 〉 80% of the variation of the resistant lines was explained by hundred seed weight and days to maturity. Cluster analysis divided the resistant lines into four groups: 21 had high seed weight (48.25 ± 3.81 g) and early maturity (95.09 ± 2.50 d), 24 had high seed weight (46.84 ± 2.10 g) and late maturity (117.00 d), 34 had low seed weight (22.35 ± 4.72 g) and early maturity (92.97 ± 3.97 d) and 31 had low seed weight (19.62 ± 5.37 g) and late maturity (112.09 ± 4.51 d).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003051100683
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 59
    Electronic Resource
    Electronic Resource
    Diversity analysis and species identification in Lens using PCR generated markers (1997)
    Springer
    Euphytica 96 (1997), S. 247-255 
    Details
    ISSN: 1573-5060
    Keywords: RAPD ; PCR ; genetic diversity ; 5S rRNA ; Lens ; lentil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using random amplified polymorphic DNA (RAPD) analysis we assessed the genetic relationships between 16 accessions and cultivars of lentil (Lens culinaris ssp. culinaris) in the Australian lentil breeding program. All lines exhibited polymorphism with a maximum dissimilarity value of 0.36. This indicated a limited degree of genetic variation. Polymerase chain reaction (PCR) with primers based on the flanking regions of the 5S rRNA gene from Pisum sativum amplified the non-translated spacer (NTS) region from within the 5S rRNA gene of Lens. Three distinct amplification banding patterns differentiated between restricted genomic DNA of Lens spp. L. culinaris ssp. culinaris and L. culinaris ssp. orientalis shared similar markers of two distinctly different NTS sizes. L. nigricans and L. odemensis shared the same amplification pattern of a single sized NTS region. However, L. ervoides contained two separate sizes of NTS, distinct from other Lens species. In an effort to widen the genetic base of cultivated lentil, these species-specific molecular markers may be used to follow potential introgression between species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003097600701
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 60
    Electronic Resource
    Electronic Resource
    Evaluation of resistance to Fusarium head blight in indigenous Japanese species of Agropyron (Elymus) (1997)
    Springer
    Euphytica 97 (1997), S. 39-44 
    Details
    ISSN: 1573-5060
    Keywords: Agropyron ; Elymus ; Fusarium head blight ; scab ; resistance ; genetic resources ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Four species of indigenous Japanese Agropyron, namely, Elymus humidus Osada (= Agropyron humidum), E. tsukushiensis Honda var. transiens (= A. tsukushiense), E. racemifer Tsvel. (= A. ciliare) and A. mayebaranum var. intermedium Hatusima were collected and evaluated for their resistance to Fusarium head blight (FHB, scab) after inoculation with a conidial suspension of Fusarium graminearum Schwabe at the flowering stage. The resistance to penetration of FHB and to fungal invasion of rachis internodes was evaluated in each accession in a comparison with six wheat cultivars. The results demonstrated that AG.91-35 of E. humidus and AG.91-24 of E. racemifer had higher resistance to penetration than the resistant wheat cultivars Nobeokabouzu-komugi and Sumai 3, respectively. All accessions of indigenous Japanese species of Agropyron examined, with the exception A. mayebaranum, had a statisticallysimilar resistance to invasion, which was the same as that of Nobeokabouzu-komugi or Sumai 3. In all accessions of E. humidus, with only one exception, no spread of the fungus from the infected spikelets to the rachis internodes was detected at all. The possible application of the strains of Agropyron as genetic resources for development of resistance to FHB in wheat is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003078109694
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 61
    Electronic Resource
    Electronic Resource
    Inheritance of resistance to two isolates of sterility mosaic pathogen in pigeonpea (Cajanus cajan (L.) Millsp.) (1997)
    Springer
    Euphytica 97 (1997), S. 45-52 
    Details
    ISSN: 1573-5060
    Keywords: Cajanus Cajan ; inheritance ; isolates ; pigeonpea ; resistance ; sterility mosaic ; susceptibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies were conducted to determine the inheritance of resistance to two isolates of the sterility mosaic pathogen, in three crosses of pigeonpea, involving resistant (ICP 7035, ICP 7349 and ICP 8850) and susceptible (ICP 8863) lines. Observations of F1 and F2 plants were explained on the basis of two independent non-allelic recessive genes for the less virulent, old Patancheru isolate (isolate 1). The backcrosses corroborated the segregation pattern observed in the F2 generation. For the more virulent, new Patancheru isolate (isolate 2), differential behavior of the F1’s was observed. Resistance was dominant in two crosses (ICP 7035 X ICP 8863 and ICP 7349 X ICP 8863), and susceptibility in the other cross (ICP 8850 X ICP 8863). The disease reaction for isolate 2, appeared to be governed by a single gene with three alleles, with one resistance allele exhibiting dominance and the other being recessive, over the allele for susceptibility. Monogenic inheritance of resistance to both isolates was noticed in the cross ICP 8850 X ICP 8863.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003013824715
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 62
    Electronic Resource
    Electronic Resource
    Variation in Capsicum annuum revealed by RAPD and AFLP markers (1998)
    Springer
    Euphytica 99 (1998), S. 167-173 
    Details
    ISSN: 1573-5060
    Keywords: AFLP ; Capsicum annuum ; genetic distances ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic relationships were examined among thirty-four pepper (Capsicum annuum) cultivars of different types. Two types of PCR-based markers were used, RAPD and AFLP, and their relative effectiveness was compared. A dendrogram based on RAPD markers separated the large-fruited sweet cultivars from the small-fruited pungent peppers, and the former group showed less divergence than the latter. The percentage of polymorphic markers was lower for AFLP than for RAPD markers (13 and 22% respectively). However, AFLP primers amplified on average six times more products than RAPD markers. The average numbers of polymorphic products per primer were 1.6 and 6.5 for RAPD and AFLP primers, respectively, i.e., AFLP primers were four times more efficient than RAPD primers in their ability to detect polymorphism in pepper. While four blocky type cultivars were indistinguishable by RAPD, two AFLP primer pairs were sufficient to distinguish the four cultivars from each other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018301215945
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 63
    Electronic Resource
    Electronic Resource
    Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status (1996)
    Springer
    Euphytica 91 (1996), S. 59-87 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat-alien translocation ; resistance ; C-banding ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wild relatives of common wheat, Triticum aestivum, and related species are an important source of disease and pest resistance and several useful traits have been transferred from these species to wheat. C-banding and in situ hybridization analyses are powerful cytological techniques allowing the detection of alien chromatin in wheat. C-banding permits identification of the wheat and alien chromosomes involved in wheat-alien translocations, whereas genomic in situ hybridization analysis allows determination of their size and breakpoint positions. The present review summarizes the available data on wheat-alien transfers conferring resistance to diseases and pests. Ten of the 57 spontaneous and induced wheat-alien translocations were identified as whole arm translocations with the breakpoints within the centromeric regions. The majority of transfers (45) were identified as terminal translocations with distal alien segments translocated to wheat chromosome arms. Only two intercalary wheat-alien transloctions were identified, one induced by radiation treatment with a small segment of rye chromosome 6RL (H25) inserted into the long arm of wheat chromosome 4A, and the other probably induced by homoeologous recombination with a segment derived from the long arm of a group 7 Agropyron elongatum chromosome with Lr19 inserted into the long arm of 7D. The presented information should be useful for further directed chromosome engineering aimed at producing superior germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035277
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 64
    Electronic Resource
    Electronic Resource
    DNA typing of hops (Humulus lupulus) through application of RAPD and microsatellite marker sequences converted to sequence tagged sites (STS) (1996)
    Springer
    Euphytica 91 (1996), S. 277-284 
    Details
    ISSN: 1573-5060
    Keywords: DNA polymorphisms ; DNA typing ; hops ; Humulus lupulus ; microsatellite DNA ; RAPD ; sequencetagged site ; STS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Both random amplified polymorphic DNA and microsatellite repeat sequences were investigated as DNA markers for distinguishing hop cultivars. Microsatellite sequences converted to STS markers proved to be most successful. The relative abundance of microsatellite repeat sequences in the hop genome varied depending on the sequence class. Of the repeat types investigated the dinucleotide repeats (GA)n and (GT)n are the most highly represented in the hop genome. Microsatellite repeat sequences in hops have been shown to be highly polymorphic and are very informatives as STS molecular markers. A DNA typing system using sequence-tagged microsatellite site markers has been developed which will not only be useful for hop cultivar identification but also marker assisted breeding and quality control purposes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033088
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 65
    Electronic Resource
    Electronic Resource
    Inheritance of resistance to Striga gesnerioides biotypes from Mali and Niger in cowpea (Vigna unguiculata (L.) Walp.) (1997)
    Springer
    Euphytica 94 (1997), S. 273-278 
    Details
    ISSN: 1573-5060
    Keywords: biotype ; control method ; cowpea ; inheritance ; resistance ; Striga gesnerioides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pot and laboratory experiments were performed in order to elucidate the genetics of resistance of three cowpea (Vigna unguiculata (L.) Walp.) resistant sources to two biotypes of the parasitic weed Striga gesnerioides (Willd.) Vatke (witchweed). B301, Suvita-2 and IT82D-849 were crossed to susceptible cultivars. Roots of each plant of parental, F1, F2, BC1 and BC2 populations were washed free of soil and examined closely for S. gesnerioides attachment. Data on resistant and susceptible cowpea plants were analyzed using the chi-square test to ascertain the goodness of fit to different genetic ratios. Segregation patterns indicate monogenic dominant inheritance of resistance to both S. gesnerioides biotypes in B301. Suvita-2, which is susceptible to the biotype from Maradi, Niger, showed segregation patterns that indicate monogenic dominant inheritance of resistance to S. gesnerioides biotype from Cinzana, Mali. Results also indicate monogenic dominant inheritance of resistance to S. gesnerioides biotype from Cinzana in IT82D-849. However, the inheritance of resistance to the biotype from Maradi would be monogenic and recessive in this line. These results suggest that the resistance of all three sources would be easy to transfer to promising breeding lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002927705470
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 66
    Electronic Resource
    Electronic Resource
    Resistance to cowpea severe mosaic virus, determined by three dosage dependent genes in Vigna unguiculata L. Walp (1997)
    Springer
    Euphytica 95 (1997), S. 49-55 
    Details
    ISSN: 1573-5060
    Keywords: cowpea severe mosaic virus ; genetics ; selection ; screening ; resistance ; tolerance ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract P1, P2, F1, F2, BC1 and BC2 generations of four resistant × susceptible crosses and three resistant × resistant crosses of cowpea (Vigna unguiculata L. Walp) were screened for resistance to cowpea severe mosaic virus (CPSMV), in an insect protected screen house. The segregation ratio, at maturity, showed a 63 susceptible : 1 resistant ratio in the F2 generation indicating that resistance is governed by three major genes. The backcross tests and the F3 test confirmed this. The intermediate levels of symptoms observed in the F1 generation and the progression of symptom expression in the F2 generation suggested that resistance is gene dosage dependent. The genetic basis of immunity, tolerance, high level of resistance is discussed based on the three gene model. Delayed symptom expression was evident in genotypes with one or two susceptible alleles. The implications of delayed expression of symptoms in selection and breeding for resistance to CPSMV are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002988003402
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 67
    Electronic Resource
    Electronic Resource
    Induction of callose formation is a sensitive marker for genotypic aluminium sensitivity in maize (1997)
    Springer
    Plant and soil 192 (1997), S. 23-30 
    Details
    ISSN: 1573-5036
    Keywords: aluminium ; callose ; protoplasts ; resistance ; toxicity ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The screening of 37 Zea mays L. cultivars in nutrient solution using root elongation (24 h) as a parameter showed large genotypic differences in Al resistance among the genetic material evaluated. Callose concentrations in root tips were closely and positively related to Al-induced inhibition of root elongation. Therefore, Al-induced callose formation in root tips appears to be an excellent indicator of Al injury and can be used as a selection criteria for Al sensitivity. In contrast, aluminium concentrations in root tips were not related to Al-induced inhibition of root elongation, nor to Al-induced callose formation. Callose formation was also induced by short-term A1 treatment in root tip protoplasts, and the response of protoplasts clearly reflected the cultivar-specific response to Al of intact roots. This indicates that in maize, Al sensitivity is expressed on the protoplast level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004204120863
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 68
    Electronic Resource
    Electronic Resource
    Effects of aluminium treatment on Norway spruce roots: Aluminium binding forms, element distribution, and release of organic substances (1999)
    Springer
    Plant and soil 216 (1999), S. 103-116 
    Details
    ISSN: 1573-5036
    Keywords: aluminium ; binding forms ; element compartmentation ; Picea abies ; resistance ; root exudates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to investigate if Al resistance in Norway spruce (Picea abies[L.] Karst.) can be attributed to similar exclusion mechanisms as they occur in several crop plants, three-year-old Norway spruce plants were treated for one week in hydroculture with either 500 μM AlCl3 or CaCl2 solutions at pH 4. Sequential root extraction with 1 M NH4Cl and 0.01 M HCl and EDX microanalysis revealed that Al and Ca in cell walls and on the surface participated in exchange processes. About half of the Al extracted by the sequential extraction was not exchangeable by 1 M NH4Cl. Phenolics and phosphate present in the root extracts are possible ligands for Al adsorbed to or precipitated at the root in a non-exchangeable form. In both treatments, C release during the first period of 2 d was much higher than during the remaining time of the experiment. Al treated plants released less total C, carbohydrates and phenolics than did Ca treated plants. Acetate was the only organic acid anion that could be detected in some samples of both treatments. Free amino acids were present at micromolar concentrations but as hydrolysis did not increase their yield, there was no evidence of peptide release. One to two thirds of the released C were large enough not to pass a 1 kDa ultrafilter. The results suggest that exudation of soluble organic complexors is not a major Al tolerance mechanism in Norway spruce, although complexation of Al by phenolic substances released by the root could be detected by fluorescence spectroscopy. Aluminium tolerance could rather be attributed to immobilization in the root apoplast, where strong binding sites are available or precipitation may occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004728122261
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 69
    Electronic Resource
    Electronic Resource
    Comparison of two crossing and four selection schemes for yield, yield traits, and slow rusting resistance to leaf rust in wheat (1998)
    Springer
    Euphytica 100 (1998), S. 35-43 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita ; leaf rust ; breeding ; resistance ; yield ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The most important breeding objectives in crop improvement are improving grain yield, grain quality, and resistances to various biotic and abiotic stresses. The objectives of our study were to compare two crossing and four selection schemes for grain yield, yield traits, and slow rusting resistance to leaf rust (Puccinia recondita) based on additive genes in wheat (Triticum aestivum), and to identify the most efficient crossing and selection methodologies in terms of genetic gains and cost efficiency. Segregating populations were derived from 18 simple crosses and the same number of top (three-way) crosses. Half of the crosses were derived from Yecora 70 and the other half from Veery #10 as the common leaf rust susceptible parents. The four selection schemes were: pedigree, modified bulk (F2 and F1-top as pedigree, selected lines in F3, F4, F2-top, F3-top as bulk; and pedigree in F5 and F4-top populations), selected bulk (selected plants in F2, F3, F4, F1-top, F2-top and F3-top as bulk; and pedigree in F5 and F4-top populations), and nonselected bulk (bulk in F2, F3, F4, F1-top, F2-top and F3-top; and pedigree in F5 and F4-top populations). A total of 320 progeny lines, parents and checks were tested for grain yield, other agronomic traits and leaf rust resistance during the 1992/93 and 1993/94 seasons in Ciudad Obregon (Sonora State, Mexico) which represents a typical high yielding irrigated site. The influence of the type of cross and the selection scheme on the mean grain yield and other traits of the progenies was minimal. The selection of parents was the most important feature in imparting yield potential and other favourable agronomic traits. Moreover, the highest yielding lines were distributed equally. Progeny lines derived from Veery #10 crosses had significantly higher mean grain yield compared to those derived from the Yecora 70 crosses. Furthermore, a large proportion of the highest yielding lines also originated from Veery #10 crosses. Mean leaf rust severity of the top cross progenies was lower than that of the simple cross progenies possibly because two parents contributed resistance to top cross progenies. Mean leaf rust severity of the nonselected bulk derivatives was twice that of lines derived from the other three schemes. Selected bulk appears to be the most attractive selection scheme in terms of genetic gains and cost efficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018391519757
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 70
    Electronic Resource
    Electronic Resource
    A molecular and embryonic study of apomixis in cassava (Manihot esculenta Crantz) (1998)
    Springer
    Euphytica 102 (1998), S. 9-13 
    Details
    ISSN: 1573-5060
    Keywords: embryo sac ; RAPD ; interspecific hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In cassava, apomixis fixes heterosis and avoids transmission of systemic pathogens which complicate vegetative propagation of the crop. A combination of evidence from maternal inheritance of RAPD markers and the structure of the embryonic sac in large progeny sets of two distinct genotypes have further confirmed the occurrence of apomixis in cassava. We could advance further on earlier reports of the detection of apomixis in four ways: (1) we could arrive at an estimate of the rate of facultative apomixis in the range of 2%; (2) we detected the occurrence of apomixis in a second genotype, derived from a different interspecific cross; (3) apomictic behavior was demonstrated in an F1 individual and (4) parallel embryonic evidence was generated that corroborate the potential occurrence of apomixis by apospory. The fact that apomixis was detected in an F1 interspecific hybrid hints to the possibility of directly transferring genes for apomixis from a wild relative to cultivated cassava.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018303109798
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 71
    Electronic Resource
    Electronic Resource
    Contrasting genetic variation amongst lentil landraces from different geographical origins (1998)
    Springer
    Euphytica 102 (1998), S. 265-273 
    Details
    ISSN: 1573-5060
    Keywords: bottleneck ; isozyme ; landrace ; lentil ; RAPD ; South Asia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lentil landraces from South Asia exhibit a low diversity and discordance with landraces from other countries according to a combination of qualitative and quantitative agromorphological characters. They exhibit specific phenological adaptation to the South Asian environment which precludes the direct use of alien germplasm in breeding programs in South Asia. An understanding of the genetic relationships and diversity of South Asian lentil landraces, in relation to landraces from other countries, is important in attempting to widen the genetic base of germplasm in the region. The objectives of this study were to investigate the genetic relationships between lentil landraces from 3 South Asian countries (India, Nepal and Pakistan) and those from 13 other countries and to determine their relative genetic diversities, using both isozyme electrophoresis and random amplified polymorphic DNA (RAPD) analysis. Polymorphisms were observed for 7 isozyme loci (16 alleles) and 22 RAPD loci. According to Nei's genetic distance, germplasm from Afghanistan clustered with that from the South Asian countries. The germplasm from these countries was striking different to that from the other countries studied. Based on genetic distance estimates from RAPD analysis, the countries with the lowest diversity were Pakistan, Afghanistan and Nepal. These data support evidence at the morphological level of a genetic bottleneck in lentil landraces from South Asia. Genetic relationships between countries outside the South Asian group are discussed. Classification into macrosperma and microsperma types did not reflect overall country relationships.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018331432580
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 72
    Electronic Resource
    Electronic Resource
    Combination of resistance to potato late blight in foliage and tubers by intraspecific dihaploid protoplast fusion (1998)
    Springer
    Euphytica 102 (1998), S. 363-370 
    Details
    ISSN: 1573-5060
    Keywords: dihaploid Solanum tuberosum ; Phytophthora infestans (Mont.) de Bary ; polygenically inherited resistances ; somatic hybrids ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Five dihaploid Solanum tuberosum genotypes, encompassing different levels of polygenically inherited resistance to potato late blight disease (Phytophthora infestans (Mont.) de Bary) in foliage and tubers, were used in four intraspecific protoplast fusion combinations. Vigorous growing putative hybrid calli were selected four weeks after electrofusion. Intact plants were regenerated from 6% of the selected calli. Verification of hybridity was accomplished by use of RAPD analysis which revealed that 53% of the regenerated plants were true somatic hybrids. The score of true somatic hybrids in the different fusion combinations ranged within 21% – 100%. The hybrid plants were analysed for resistance to foliage blight in a field trial and assessed for tuber blight resistance by use of a laboratory test. Resistance to late blight in foliage and tubers varied between the hybrids. Very high levels of resistance to both foliage and tuber blight were obtained in some hybrids. However, loss of resistance in some hybrids as compared to the parental plants were also observed. Possible reasons for the phenotypic disappearance of resistance to either foliage or tuber blight are suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018302103100
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 73
    Electronic Resource
    Electronic Resource
    Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers (1999)
    Springer
    Euphytica 109 (1999), S. 107-115 
    Details
    ISSN: 1573-5060
    Keywords: AFLP ; DNA fingerprinting ; isozymes ; RAPD ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A survey of the genetic diversity among the major cuban rice cultivars was conducted using isozyme, RAPD and AFLP markers. Polymorphisms were detected for esterases, peroxidases, alcohol dehydrogenases and polyphenoloxidases systems; 21 RAPD primers and four AFLP primer combinations. Heterozygosity arithmetic mean value (Hav(p)), the effective multiplex ratio (EMR) and the marker index (MI), were calculated for isozyme, RAPD and AFLP markers. The mean value of genetic similarity among the different varieties was 0.92 for isozyme, 0.73 for RAPD and 0.58 for AFLP analyses. Thus, AFLP were able to detect polymorphisms with higher efficiency than RAPD (+15%) and isozyme (+34%). Data from the isozyme, RAPD and AFLP analyses were used to compute matrices of genetic similarities. The efficiency of the UPGMA for the estimation of genetic relatedness among varieties was supported by cophenetic correlation coefficients. The resulting values indicated that the distortion level for the estimated similarities was minimal. The correlation coefficients obtained by the Mantel matrix correspondence test, which was used to compare the cophenetic matrices for the different markers, showed that estimated values of genetic relationship given for isozyme and RAPD markers (r = 0.89), as well as for AFLP and RAPD markers (r = 0.82) were properly related. However, AFLP and isozyme data showed only moderate correlation (r = 0.63). Although the genetic variability found among the different cultivars was low, both RAPD and AFLP markers proved to be efficient tools in assessing the genetic diversity of rice genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003672729948
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 74
    Electronic Resource
    Electronic Resource
    Genetic diversity of plums characterized by random amplified polymorphic DNA (RAPD) analysis (1999)
    Springer
    Euphytica 109 (1999), S. 143-147 
    Details
    ISSN: 1573-5060
    Keywords: classification ; DNA ; plum varieties ; Prunus ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We investigated the genetic diversity of 42 plum varieties by RAPD analysis. Twenty primers discriminated all plum varieties excepting two synonymous pairs: 'Botankyou and ‘Kelsey’, and ‘Chairn’ and ‘Tragedy’. Two North American plums, ‘Beach Plum’ and ‘Glow’, were genetically distinct from the other plums by cluster analysis. Overlaps observed between the ‘European plum group’ and the ‘Japanese plum group’, were perhaps due to intercrossing. We could also discriminate ‘Sordum’ from 'Late Sordum and ‘Bansei Sordum’, although ‘Late Sordum’ and ‘Bansei Sordum’ are thought to be derived from bud mutants of ‘Sordum’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003728201100
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 75
    Electronic Resource
    Electronic Resource
    Combining ability of resistance to head blight caused by Fusarium culmorum (W.G. Smith) in the F1 of a seven parent diallel of winter wheat (Triticum aestivum L.) (1999)
    Springer
    Euphytica 110 (1999), S. 199-206 
    Details
    ISSN: 1573-5060
    Keywords: diallel analysis ; Fusarium culmorum ; heterosis ; resistance ; scab ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fusarium head blight (FHB, scab) caused by Fusarium spp. is a widespread disease of cereals causing relevant yield and quality losses and contaminating cereal products with mycotoxins. Breeding resistant cultivars is the method of choice for controlling the disease. Resistance to FHB is a quantitative trait and is most likely governed by several genes. We present the results of an F1 diallel analysis of FHB resistance involving six resistant and one susceptible European winter wheat genotypes of diverse origin in order to identify promising combinations for the selection of improved cultivars. Parents and F1s including reciprocals were evaluated for FHB resistance in an artificially inoculated field trial. Two traits were assessed: visual disease symptoms on the heads and the percentage of Fusarium damaged kernels in a harvested sample. General combining ability (GCA) and specific combining ability (SCA) effects were statistically significant for visual symptoms and kernel damage, whereas reciprocal effects were small or not significant. Heterosis for resistance was common, indicating that the parental genotypes possess different resistance genes. Selection of transgressive segregates should be feasible from such heterotic combinations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003757002052
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 76
    Electronic Resource
    Electronic Resource
    Genetic diversity for RAPD markers between cultivated and wild accessions of Coffea arabica (1996)
    Springer
    Euphytica 87 (1996), S. 59-64 
    Details
    ISSN: 1573-5060
    Keywords: Coffea arabica ; coffee ; diversity ; genetic resources ; heterosis ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Random amplified polymorphic DNA (RAPD) markers have been successfully employed to analyse the genetic diversity among cultivated and subspontaneous accessions of Coffea arabica. The narrow genetic base of commercial cultivars was confirmed. On the other hand, a relatively large genetic diversity was observed within the germplasm collection demonstrating the importance of collecting missions. Results suggested an East-West differentiation in Ethiopia, the primary centre of diversification of C. arabica. The large heterosis effect reported in intergroup hybrids could be related to such genetic differentiation. RAPD method appeared to be effective in resolving genetic variations and in grouping germplasm in C. arabica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022965
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 77
    Electronic Resource
    Electronic Resource
    Variation in the response of melon genotypes to sudden wilt (1996)
    Springer
    Euphytica 87 (1996), S. 91-95 
    Details
    ISSN: 1573-5060
    Keywords: Collapse ; cucumis melo ; melon ; resistance ; sudden wilt ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Differences in the response of melon genotypes to the sudden wilt disease were observed in several field trials conducted during 1993–1994 in the Arava region of southern Israel. Generally, the disease was more severe in the late summer growing season which is shorter and has higher temperatures than the spring and autumn growing seasons. The Oriental pickling melon breeding line P6a was the most tolerant among the entries tested. The response to the disease was also studied using two segregating families and their progenitors. BSK (tolerant) × P202 (susceptible) and P6a (tolerant) × D17 (susceptible). Wilting percentages of F1, F2 and backcross families were intermediate between the parents, suggesting an additive mode of gene action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021880
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 78
    Electronic Resource
    Electronic Resource
    Genetic diversity in Lima bean (Phaseolus lunatus L.) as revealed by RAPD markers (1997)
    Springer
    Euphytica 95 (1997), S. 157-165 
    Details
    ISSN: 1573-5060
    Keywords: andean ; cultigroups ; genetic variability ; Lima bean ; mesoamerican ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic variability of 46 accessions of the Lima bean (P. lunatus L.) including 16 wild forms and 30 landraces belonging to the three cultigroups Big lima, Sieva, Potato, and their intermediates, was evaluated using RAPD (Random amplified polymorphic DNA) markers. Twelve oligonucleotide primers produced 172 RAPD markers which allowed the differentiation of two main groups: the mesoamerican and the andean groups. This was confirmed by an AMOVA analysis which indicated that 37.7% of the variation was found between these two groups. For each botanical form (wild and cultivated), the molecular markers showed that small-seeded types (i.e. Sieva and Potato types and their related wild forms) had a wide distribution (from Mexico to Argentina) while the large-seeded types (Big lima type and its related wild forms) were circumscribed to the narrow west-coastal region from Ecuador to Bolivia. The results are in favour of an independent domestication process within the two groups, as the differentiation between mesoamerican and andean accessions was found to occur in both wild forms and landraces. Within each of the two main groups, wild forms and landraces were also found to be genetically differentiated and higher genetic diversity was observed among landraces than among wild forms. Within the mesoamerican landraces, low but significant differentiation between the Sieva and Potato cultigroups was observed. Some suggestions and hypotheses are discussed about evolution of the two small-seeded types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002910114869
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 79
    Electronic Resource
    Electronic Resource
    Genetic variation of mitochondrial and nuclear genomes in carrots revealed by random amplified polymorphic DNA (RAPD) (1997)
    Springer
    Euphytica 95 (1997), S. 259-267 
    Details
    ISSN: 1573-5060
    Keywords: cytoplasmic male sterility ; Daucus carota ; ssp. sativus ; mitochondrial DNA ; pedigree analysis ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mitochondrial and nuclear genomic diversities of 8 carrot (Daucus carota ssp. sativus) varieties, including 6 pure lines and 2 cytoplasmic male sterile (cms) lines, were taxonomically identified using PCR with 19 RAPD primers. Dendrograms based on polymorphisms of both mitochondrial and nuclear genomes were constructed. According to the dendrogram of the mitochondrial genome revealed by RAPD, 4 differentiated clusters formed, in good accordance with the classification based on analyses with restriction enzyme digestion. Two cms lines were grouped into the same cluster, as genetically separated from the others. Thus, the cytoplasm donors of these male sterile lines were thought to be wild carrots. Conversely, RAPD analysis of the nuclear genome for these eight cultivars revealed no evident clusters although some cultivars were of a similar origin or place of cultivation. A correlation between nuclear and mitochondrial dendrograms was absent. RAPD has proved to be a useful tool for identifying mitochondrial and nuclear genomes. This technique will greatly aid in promoting efficient improvement of carrots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002901112164
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 80
    Electronic Resource
    Electronic Resource
    The use of Rapd markers for lentil genetic mapping and the evaluation of distorted F2 segregation (1997)
    Springer
    Euphytica 96 (1997), S. 405-412 
    Details
    ISSN: 1573-5060
    Keywords: Lens culinaris ; linkage ; RAPD ; segregation distortion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To maximize the extent of polymorphism within a mapping population wide crosses are often made, frequently resulting in distorted segregation. Two parents used in the crosses in this study contained ca 50% from wild lentil genome (Lens culinaris ssp. Orientalis). We investigated the use of random amplified polymorphic DNA (RAPD) in the lentil (Lens culinaris Med.), for genetic mapping and testing for segregation distortion in F2 populations. In cross 1, 83% of the RAPD markers showed segregation distortion, which was also observed for isozyme and morphological loci. By contrast, in cross 2, there was little (10%) segregation distortion. Out of 390 primers tested, 116 primers (29.7%) yielded 192 polymorphic fragments between parents of cross 2. This polymorphism was confirmed as reproducible. Seventy-eight segregating loci were analyzed for linkage, at a LOD score 〉 3.0, resulted in 28 RAPD, one RFLP, one morphological and three oligonucleotide markers, which were assigned to 9 linkage groups spanning 206 cM. Clearly, in lentil RAPD markers were valuable for genetic mapping and evaluation of segregation distortion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003045000568
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 81
    Electronic Resource
    Electronic Resource
    Resistance to Karnal bunt in Hordeum chilense and its amphiploids with Triticum species (1997)
    Springer
    Euphytica 96 (1997), S. 327-330 
    Details
    ISSN: 1573-5060
    Keywords: amphiploids ; Karnal bunt ; resistance ; Tilletia indica ; tritordeum ; Hordeum chilense
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Reactions of Hordeum chilense accessions H1 and H7 and their amphiploids, HT8, HT9 and HT28 (named as tritordeum) alongwith wheat lines, T22, T24 and T59 used in their synthesis, were studied for resistance to the Karnal bunt pathogen (Tilletia indica) of wheat. Both the accessions of H. chilense and one tritordeum line, HT8, were rated as highly resistant with zero co-efficient of infection, whereas the other two tritordeum lines HT28 and HT9 were rated as moderately susceptible and susceptible with 5.2 and 10.5 co-efficients of infection, respectively, compared to reaction of the wheat lines involved in their synthesis. Karnal bunt infection was maximum on the susceptible wheat cultivar WL-711 with 24.3 co-efficient of infection. All the wheat lines involved in the synthesis of amphiploids were susceptible to Karnal bunt except, T59 (Triticum sphaerococcum) (6X), which showed a moderate level of resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003019326395
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 82
    Electronic Resource
    Electronic Resource
    The diversity of head-scab resistance in Triticeae and their relation to ecological conditions (1997)
    Springer
    Euphytica 97 (1997), S. 277-281 
    Details
    ISSN: 1573-5060
    Keywords: head scab ; resistance ; Triticeae ; Fusarium graminearum ; ecological conditions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract 1507 accessions from 93 species of 18 genera in Triticeae were screened for resistance to initial infection and resistance to pathogen spread with multi-floret and single-floret injection inoculation methods respectively. The accessions with high resistance were mainly found in perennial genera: Roegneria, Hystrix, Agropyron, Kengyilia and Elymus. Based on differences in resistance, 18 genera screened in Triticeae could be classified into five groups. The species with high resistance were mainly distributed in humid ecological environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003028324059
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 83
    Electronic Resource
    Electronic Resource
    Geographical distribution of genetic variation in Stylosanthes scabra revealed by RAPD analysis (1997)
    Springer
    Euphytica 98 (1997), S. 21-27 
    Details
    ISSN: 1573-5060
    Keywords: Stylosanthes scabra ; genetic variation ; geographical distribution ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A large number of S. scabra accessions have been accumulated worldwide. The majority of them were collected from Brazil and most of the others came from either Colombia or Venezuela. One hundred of these accessions, selected to represent the geographical distribution of the S. scabra collection held at the Australian Tropical Forages Genetic Resource Centre, were analysed using RAPD as markers. Seven of these accessions were found not to be S. scabra. Of the S. scabra accessions, the average dissimilarity value among Brazilian accessions (0.053) was much lower than that among Colombian (0.074) or Venezuelan (0.088) accessions, with an overall dissimilarity value of 0.059 among all the S. scabra accessions. Based on their dissimilarity values, most of these accessions could be separated into five groups. Geographical distributions for most accessions in each of these groups were well defined. Limited long distance introductions/dispersions of S. scabra between these regions were detected and they were mainly confined to Brazilian genotypes. The clustering results based on RAPD were compared with those based on morphological-agronomical characters, and the groups produced by the two different methods did not always match. Possible reasons for this discrepancy are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003026915825
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 84
    Electronic Resource
    Electronic Resource
    Molecular characterization of taro (Colocasia esculenta) using RAPD markers (1998)
    Springer
    Euphytica 99 (1998), S. 183-189 
    Details
    ISSN: 1573-5060
    Keywords: taro ; Colocasia esculenta ; RAPD ; DNA fingerprinting ; phylogenetic tree
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Forty-four taro (Colocasia esculenta), two tanier (Xanthosoma species) and one Colocasia gigantea accessions were evaluated for genetic diversity using random amplified polymorphic DNA (RAPD) primers. Seventy-three of 112 primers amplified PCR DNA products used to fingerprint the accessions. Thirty-two primers were considered highly informative because they amplified more than 5 bands or amplified one or more polymorphic bands that distinguished between accessions. RAPDs showed high genetic diversity in taro accessions from Indonesia, were capable in distinguishing between Hawaiian accessions, and could separate triploid from diploid accessions. UPGMA cluster analysis of genetic similarity estimates (Jaccard's coefficient), separated the accessions into 3 main groups with C. esculenta divided into 5 subgroups. These primers will be useful for future genetic analysis and provide taro breeders with a genetic basis for selection of parents for crop improvement. Polymorphic markers identified in the DNA fingerprinting study will be useful to screen a segregating population which is being generated in our laboratory aimed at developing a taro genetic linkage map.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018309417762
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 85
    Electronic Resource
    Electronic Resource
    Identification of RAPD markers linked to linolenic acid genes in rapessed (1996)
    Springer
    Euphytica 90 (1996), S. 351-357 
    Details
    ISSN: 1573-5060
    Keywords: doubled haploid ; gene mapping ; genetic control ; linolenic acid ; mutant ; plant breeding ; RAPD ; rape seed ; Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The inheritance of the low linolenic acid content (derivated from mutant lines) in rapeseed was investigated. Molecular techniques of gene mapping through RAPD markers were applied on a microspore-derived progeny from a high × low linolenic acid F1 hybrid. ‘Bulked segregant analysis’ made it possible to test rapidly number of RAPD primers. Two linkage groups of 6 markers (72.7 cM and 75.6 cM) were determined. Each corresponded to a major QTL which explained 24% and 30.7% of the total phenotypic variation of the linolenic acid content. It was confirmed that two independant mutations were implied in the low linonenic acid content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027487
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 86
    Electronic Resource
    Electronic Resource
    The taxonomic characterisation of annual Beta germplasm in a genetic resources collection using RAPD markers (1996)
    Springer
    Euphytica 91 (1996), S. 205-212 
    Details
    ISSN: 1573-5060
    Keywords: Beta ; genetic resources ; RAPD ; annual beets ; molecular marker ; polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Annual beets in the genus Beta section Beta represent an important genetic resource. Representative accessions of annual beets from a beet germplasm collection were analysed using RAPD to assess the patterns of variation and relationships among them. Using arbitrary primers, markers showing variation across accessions were identified. A dendrogram of similarity was produced using these molecular markers. All the accessions analysed were classified into three major groups corresponding to species or subspecies macrocarpa, adanensis and maritima. Macrocarpa was shown to be the most divergent group in this section. Using RAPD molecular markers, it was possible to ascribe an accession to one of three taxonomic groups and overcome much of the confusion encountered when morphological traits are used for identification. The group of maritima was found to be more polymorphic than either the group of macrocarpa or adanensis at both accession and subspecies levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021071
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 87
    Electronic Resource
    Electronic Resource
    Resistance of nineteen cultivars of subterranean clover to four races of Phytophthora clandestina (1996)
    Springer
    Euphytica 91 (1996), S. 351-358 
    Details
    ISSN: 1573-5060
    Keywords: Phytophthora clandestina ; resistance ; root rot disease ; subterranean clover ; Trifolium subterraneum ; virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The resistance of 19 cultivars of subterranean clover was screened against 4 races of P. clandestina by mycelial inoculation of roots of 10-day-old seedlings growing in water agar and by growing seedlings in pasteurised potting mix containing infested vermiculite in controlled conditions. The cultivars showed differential resistance (vertical resistance) to races of the pathogen and can be divided into 4 resistance groups. Cultivars Clare, Esperance, Green Range, Junee, Mount Barker, Rosedale, Woogenellup and Yarloop were susceptible to all races. Cultivars Bacchus Marsh, Denmark, Enfield, Gosse, Goulburn, Karridale, Larisa, Leura and Trikkala were susceptible to races 1 and 3, but resistant to races 0 and 2. Cultivar Meteora was susceptible to races 2 and 3, but resistant to races 0 and 1. Cultivar Seaton Park (LF) was resistant to all races. Cultivars also varied in their race-non-specific (horizontal) resistance: cultivars that were susceptible to particular races usually varied in their degree of susceptibility to those races. In particular, Junee was more resistant to all four races than the other cultivars within its group. Similarly, cultivars Gosse, Larisa, Denmark and Trikkala were more resistant to races 1 and 3 than the other cultivars in their group. Races of the pathogen varied in their aggressiveness as well as in their virulence, as shown by the variation in aggressiveness of different isolates of race 0.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033097
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 88
    Electronic Resource
    Electronic Resource
    Diversity among Finnish net blotch isolates and resistance in barley (1996)
    Springer
    Euphytica 92 (1996), S. 81-87 
    Details
    ISSN: 1573-5060
    Keywords: barley ; net blotch ; Pyrenophora teres ; resistance ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seedlings of a differential barley (Hordeum vulgare L.) series (21 genotypes) and of six check genotypes were used in the greenhouse to assess variation in virulence among 20 single-spore isolates of the net blotch pathogen. Pyrenophora teres Drechs. f.teres Smedeg., collected from various sites in Finland. The experiment was run twice and symptom expression was recorded on the first three leaves. Analysis of second leaf symptom scores from Run 1 indicated differences in virulence between isolates, all of which were pathogenic, and differential resistance among the barleys. The virulence of P. teres isolates appeared to be conditioned by the host barley from which the isolate derived; the average virulence of isolates collected from a susceptible host was greater than that of isolates collected from a less susceptible host. Results from Run 2 were similar regarding resistance within the barleys, but variation in virulence among the P. teres isolates was not consistent with that from Run 1. CI 9819 caries duplicate genes for resistance to P. teres (Rpt1b and Rpt1c), and CI 7548 possesses Rpt3d. Both genotypes were highly resistant to all isolates. The Rpt1a gene of Tifang (CI4407) conferred resistance to all isolates in Run 2, but only to about half of the isolates in Run 1. The checks, including two of the symptomatically most resistant Nordic barley genotypes, were universally susceptible during these stringent tests. No selective pressure has been placed on Finnish isolates of P. teres through previous deployment of major resistance genes, and it is speculated that any variation in virulence among isolates is likely to be due to a combination of evolutionary forces including, natural selection, random genetic drift and gene flow.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022832
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 89
    Electronic Resource
    Electronic Resource
    Genetic suppression of the cereal rye-derived gene Pm8 in wheat (1997)
    Springer
    Euphytica 93 (1997), S. 353-360 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Secale cereale ; powdery mildew ; Erysiphe graminis f.sp. tritici ; resistance ; suppression ; segregation distortion ; wheat ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The location of wheat gene Pm8 for resistance to powdery mildew in the 1RS chromosome segment derived from cereal rye cultivar Petkus was confirmed. There was reduced transmission of the 1BL.1RS chromosome relative to its wheat homologue in four of the five crosses examined. Pm8 was not expressed in some wheat cultivars and selections which possessed the 1RS chromosome identified by the presence of relevant genes for resistance to the three rusts, due to the presence of a dominant suppressor gene(s) in the wheat genome. The frequency of suppression in 1BL.1RS wheats from Mexico was significantly higher than in a group of wheats (both local and exotic) introduced from China and was probably much higher than in European wheats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002923030266
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 90
    Electronic Resource
    Electronic Resource
    Genetical studies of resistance to Phytophthora porri in Allium porrum, using a new early screening method (1997)
    Springer
    Euphytica 93 (1997), S. 345-352 
    Details
    ISSN: 1573-5060
    Keywords: Allium porrum ; autotetraploidy ; introgression ; Phytophthora porri ; resistance ; screening methods ; leek
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new screening method was developed to evaluate resistance of leek (Allium porrum) to Phytophthora porri, based on inoculation by 24 h-immersion of leek plantlets in the 3–6 leaf stage in a suspension of ca. 100 zoospores.ml-1. The immersion test was used for identifying new sources of resistance and to determine the genetic basis of resistance. Within winter leek, five resistance classes were defined on the basis of average field scores of 21 plants. Clones from these plants were tested with the immersion technique. The average scores per resistance class in immersion and field tests were significantly correlated (P〈0.01). The correlation of single-date field scores with the immersion test scores was better in the second half of the epidemic season. A selection experiment yielded a strong response to selection for resistance (53–97%) but no response to selection for susceptibility. This may indicate that resistance is due to few recessive genes in the studied winter leek. Crosses between landraces and winter leek were analyzed by means of F2 (selfed F1) and BC1, progenies. This analysis indicated the presence of few loci with dominant genes for resistance in accession CGN 873243, and additive polygenes in accession Pl 368351.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002900717062
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 91
    Electronic Resource
    Electronic Resource
    Sources of resistance to head scab in Triticum (1997)
    Springer
    Euphytica 94 (1997), S. 31-36 
    Details
    ISSN: 1573-5060
    Keywords: Triticum ; Fusarium graminearum ; resistance ; head scab ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Multi-floret and single-floret injection inoculation methods were used to test 1076 accessions of Triticum for resistance to initial infection and resistance to pathogen spread within spike tissue respectively. The data obtained between years or inoculation dates were comparatively similar and were little influenced by climatic factors, especially humid condition. All the tested materials uniformly exhibited susceptibility to initial infection, but there were great differences in resistance to spread among species or cultivars. The diploid and tetraploid wheats were severely susceptible. Only 30 genotypes of common wheat of T. aestivum concv. vulgare showed high resistance to spread. The highest frequency of high spread resistance existed in these landraces which were distributed in Zhejiang, Hubei, Hunan, Jiangsu provinces and Shanghai City, Guizhou, Sichuan and Yunnan provinces ranked second. The majority of wheat landraces from Hebei, Shanxi, Shaanxi Province and Beijing City showed susceptibility or high susceptibility. No resistance was found in Tibetian wheats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002982005541
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 92
    Electronic Resource
    Electronic Resource
    Inheritance of sethoxydim resistance in foxtail millet, Setaria italica (L.) Beauv. (1997)
    Springer
    Euphytica 94 (1997), S. 69-73 
    Details
    ISSN: 1573-5060
    Keywords: foxtail millet ; herbicide ; resistance ; interspecific hybridization ; sethoxydim ; inheritance ; Setaria italica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Interspecific hybridization between foxtail millet cultivars (Setaria italica) and a green foxtail (S. viridis) resistant to the herbicide sethoxydim were undertaken to breed foxtail millet for improved herbicide resistance. Parents, reciprocal F1 hybrids, F2 selfed derived populations and BC1 backcross progeny were produced and analysed for mortality and fresh weight over a range of dosages. All resistant progeny were 700 times more resistant than susceptible cultivars and was symptom free under current field dosages. Segregations of resistant and susceptible progeny (3:1 in F2 and 1:1 in BC1) were not dependent upon dosage. Heterozygous individuals displayed the same magnitude of resistance as homozygous plants at twelve times the recommended field dosage. Results suggested that sethoxydim resistance in foxtail millet was controlled by a single, completely dominant, nuclear gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002989725995
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 93
    Electronic Resource
    Electronic Resource
    Resistance to western flower thrips in greenhouse cucumber: effect of leaf position and plant age on thrips reproduction (1997)
    Springer
    Euphytica 94 (1997), S. 63-67 
    Details
    ISSN: 1573-5060
    Keywords: Cucumis sativus ; Frankliniella occidentalis ; plant age ; leaf position ; reproduction ; resistance ; cucumber ; western flower thrips
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three greenhouse cucumber (Cucumis sativus L.) accessions, previously selected for low levels of damage after infestation with Frankliniella occidentalis (Pergande), were tested for resistance against F. occidentalis in a no-choice greenhouse experiment at the mature plant stage. The three accessions showed a strong reduction in thrips damage compared to the susceptible control. The effects of leaf position and plant age of the four cucumber accessions on the reproduction of F. occidentalis were determined in a leaf disc assay. Leaf position had a significant effect on thrips reproduction, whereas plant age, within the test range, had not. In general, reproduction was higher on young leaves. Reproduction as measured on leaf discs from certain leaf positions was strongly correlated with resistance of mature plants in the greenhouse, and can therefore be used as a quick test to screen cucumber accessions for resistance against thrips.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002937709157
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 94
    Electronic Resource
    Electronic Resource
    Host-plant resistance to Pseudomonas solanacearum in tomato germplasm (1996)
    Springer
    Genetic resources and crop evolution 43 (1996), S. 569-574 
    Details
    ISSN: 1573-5109
    Keywords: bacterial wilt ; germplasm evaluation ; Lycopersicon esculentum ; Pseudomonas solanacearum ; resistance ; tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seedlings of two hundred and thirty-three accessions of the tomato collection maintained at the Centro Agronómico Tropical de Investigación y Enseñanza, Turrialba, Costa Rica (CATIE) and 7 cultivars used as controls were evaluated for host-plant resistance to 4 virulent strains of Pseudomonas solanacearum representing race 1 biovars 1 and 3. In general, biovar 3 strains wilted seedlings faster than biovar 1 strains but, after 20 days post-inoculation, no significant differences were noted in susceptible control ratings. Significant differences for disease index were noted, but no line with complete resistance was found. For the USA biovar 1 strain UW-25, only 5 accessions, CATIE 17331, 17334, 17349, 17739, 17740, and 2 of the control cultivars, ‘Hawaii 7998’ and ‘UC-82B’ showed some degree of resistance. Conversely, both the frequency and the degree of resistance were high for Costa Rican biovar 1 strain UW-256. For biovar 3, the Costa Rican strain UW-255 was more virulent than the Peruvian strain UW-130. Eight CATIE accessions, 5539, 17331, 17333, 17334, 17345, 17349, 17742, and MIP-CH1, were as resistant as the resistant control ‘Hawaii 7998’ to 3 strains and accession 17740 was as resistant as ‘Hawaii 7998’ to all 4 strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00138833
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 95
    Electronic Resource
    Electronic Resource
    Genotypic variation in field resistance of Cucurbita pepo cultivars to powdery mildew (Erysiphe cichoracearum) (1996)
    Springer
    Genetic resources and crop evolution 43 (1996), S. 79-84 
    Details
    ISSN: 1573-5109
    Keywords: Cucurbita pepo ; squash ; genetic resources ; Erysiphe cichoracearum ; cucurbit powdery mildew ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifty-eight cultivars of Cucurbita pepo (mostly zucchini type) were studied in three-year experiments for field resistance to powdery mildew caused by Erysiphe cichoracearum. No genotypes were found with complete resistance to powdery mildew. There were significant differences in the expression of field resistance characterized by some basic epidemiological parameters (maximum infection degree, area below curve). The highest level of field resistance occurred in 13 cultivars including Acceste F1, Albina, ambassador F1, CU-235, Elite F1, Goldfinger, Parmanta F1, Seneca Hybr. F1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00126944
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 96
    Electronic Resource
    Electronic Resource
    Origin of some minor vegetatively propagated Allium crops studied with RAPD and GISH (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 511-523 
    Details
    ISSN: 1573-5109
    Keywords: Allium ; crop evolution ; GISH ; hybridogenic cultivars ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three vegetative crops of sect. Cepa in genus Allium (Top onion, French grey shallot and viviparous triploid onion) of suspected hybridogenic origin were studied with genomic in situ hybridization (GISH) and random amplified polymorphic DNA (RAPD) markers. Related wild and cultivated species were included in the analysis in order to assess their contributions to the genomes of the investigated species. In A. × proliferum, the parental chromosomes derived from A. fistulosum and A. cepa were unequivocally identified by GISH, proving the hybrid status of this crop. The French grey shallot proved to belong to A. oschaninii according to the RAPD analysis and the GISH results, it is clearly separate from the normal shallots of A. cepa var. aggregatum. Thus the grey shallots are a new crop species and can be considered as an aggregatum form of the wild progenitor species A. oschaninii. The triploid viviparous onion comprises mostly A. cepa derived DNA in its genome. A non-cepa component could not be attributed to any of the sect. Cepa species included in this study and is most likely derived from one of the species of this section not yet identified by molecular means.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008647700251
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 97
    Electronic Resource
    Electronic Resource
    First source of resistance in durum wheat to Hessian fly (Diptera: Cecidomyiidae) in Morocco (1999)
    Springer
    Genetic resources and crop evolution 46 (1999), S. 107-109 
    Details
    ISSN: 1573-5109
    Keywords: antibiois ; Mayetiola destructor ; resistance ; Triticum turgidum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hessian fly, Mayetiola destructor (Say), is the major pest of wheat in North Africa. In Morocco, durum wheat (Triticum turgidum L. subsp. durum (Desf). Husn.) losses due to this pest have been estimated at 32%. Genetic resistance is the only economical and practical means of controlling this insect. Field and greenhouse screening of durum wheat genotypes resulted in the identification of one source of resistance to Hessian fly in Morocco. This is the first source of durum wheat Hessian fly-resistance identified in Morocco. This source of resistance expresses a medium level of antibiosis against first-instar Hessian fly larvae; about 25% of the larvae survive on resistant plants. The deployment of varieties that allow for larval survival on resistant plants should reduce selection for biotype development. This source of resistance is being used by CIMMYT/ICARDA and Moroccan breeders to develop resistant durum wheat varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008615515704
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 98
    Electronic Resource
    Electronic Resource
    Sources of resistance to bacterial blight of stock (Matthiola incana R. Br.) (1995)
    Springer
    Genetic resources and crop evolution 42 (1995), S. 371-372 
    Details
    ISSN: 1573-5109
    Keywords: bacterial blight ; interspecific hybridisation ; Matthiola ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Accessions ofM. tricuspidata (L.) R. Br.,M. aspera Boiss. andMatthiola longipetala (Vent.) DC. were tested for their reaction to bacterial blight of stock (M. incana R. Br.), caused byXanthomonas campestris pv.incanae. Potted plants were sprayed with bacterial cell suspension, adjusted to 108 cells/ml. The tested accessions of the three species were found highly resistant, while control plants ofM. incana were severely infected. InterspecificF 1 hybrid plants betweenM. incana andM. tricuspidata also exhibited high resistance to the disease. The implications and the expected difficulties in breeding stock for bacterial blight resistance are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02432141
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 99
    Electronic Resource
    Electronic Resource
    Genetic variation in common and runner bean of the Northern Meseta in Spain (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 243-251 
    Details
    ISSN: 1573-5109
    Keywords: Phaseolus ; genetic variability ; amino acids ; isozymes ; proteins ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic variation within and between Spanish landraces or varieties of Phaseolus vulgaris L. (common bean) and P. coccineus L. (runner bean) has been estimated by means of isozymes and random amplified polymorphic DNA (RAPD) analyses. Likewise, storage protein and amino acid content in dry seeds have been estimated. Fifteen landraces (60 accessions) of P. vulgaris and six of P. coccineus (six accessions) have been studied. Of the seven isozymatic systems analyzed only three systems and three loci showed variability in each species. Isozyme analyses revealed that genetic variability within and between landraces exist in both species. Even variability within accession was detected in some P. vulgaris landraces. Comparison of isozyme data indicated that Spanish landraces have a lower level of genetic variability than wild American materials and probably also lower than American landraces. RAPD analysis allowed for the uniquely distinguishing of all landraces. Genetic similarity among landraces, estimated by both isozymes and RAPDs, were not related with the seed morphological characters (color, size and shape) which define each variety or landrace. Variation in protein and amino acid content among landraces was also detected. The average protein content in common bean (20.48%) was similar to values previously reported in this species and higher than the average in the runner bean landraces (16.33%). In relation to the amino acid content methionine and cysteine showed the lowest values in all samples, although the content of these two amino acids varied widely among landraces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008604127933
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 100
    Electronic Resource
    Electronic Resource
    RAPD variation in sweetpotato (Ipomoea batatas (L.) Lam) cultivars from South America and Papua New Guinea (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 271-277 
    Details
    ISSN: 1573-5109
    Keywords: DNA fingerprints ; genetic diversity ; Ipomoea batatas ; polymerase chain reaction ; RAPD ; sweetpotato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The island of New Guinea is considered a secondary center on diversity for sweetpotato, because of its range of isolated ecological niches and large number of cultivars found within a small area. Information of genetic diversity in Papua New Guinea (PNG) sweetpotato is essential for rationalizing the global sweetpotato germplasm collection. Using random amplified polymorphic DNA (RAPD), we compared the genetic variation and genetic diversity in 18 PNG cultivars versus 18 cultivars from South America. The analysis of molecular variance revealed large genetic diversity in both groups of cultivars. The within-group (among individuals) variation accounted for 90.6% of the total molecular variance. However, the difference between PNG and South American groups is statistically significant, although it explained only 9.4% of the total molecular variance. The PNG cultivars are also less divergent than their South American ancestors as the mean genetic distance in PNG group is significantly smaller than that of South American group. The lower level of genetic diversity in PNG cultivars was also reflected by multidimensional scaling. This study shows that PNG cultivars, after many years of isolated evolution in an unique agro-ecological environment are substantially divergent from their ancestors in South America. The genetic diversity level in PNG cultivars is significantly lower than that in South American cultivars. It thus provides a baseline for continuing studies of genetic diversity in different sweetpotato gene pools.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008642707580
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...