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Cell wall pectin content modulates aluminium sensitivity of Zea mays (L.) cells grown in suspension culture (2000)

Schmohl, N. ; Horst, W. J.

Oxford, UK : Blackwell Science Ltd

Plant, cell & environment 23 (2000), S. 0

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ISSN: 1365-3040

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The pectin content of the cell walls of maize suspension cells was modified to investigate its role in the expression of aluminium (Al) toxicity. Long-term adaptation to NaCl or to the cellulose synthesis inhibitor 2,6-dichlorbenzonitirle (DCB) increased the pectin content by 31 and 86%, respectively. Subculturing salt-adapted cells for up to 3 weeks without NaCl supply or treatment of cells with pectolyase for up to 15 min reduced pectin contents by up to 46%. Such pre-cultured cells were incubated for 2 h in presence of Al. There was a close positive correlation between pectin and both total and BaCl2 non-exchangeable Al contents. Aluminium-induced callose formation as an indicator of Al injury was closely positively correlated to the loss of cell viability. In NaCl-adapted and pectolyase-treated cells, Al-induced callose formation was reduced when compared with normal cells. However, there was a close positive relationship between pectin contents and relative callose induction (digitonin-induced callose formation, reflecting the different capabilities of cells to synthesize callose set to 100%) indicating that cells with higher pectin contents are more Al-sensitive. The results presented support our view that the binding of Al to the cell wall pectin-matrix represents an important step in the expression of Al toxicity.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1365-3040.2000.00591.x

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Induction of callose formation is a sensitive marker for genotypic aluminium sensitivity in maize (1997)

Horst, W.J. ; Püschel, A.-K. ; Schmohl, N.

Springer

Plant and soil 192 (1997), S. 23-30

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ISSN: 1573-5036

Keywords: aluminium ; callose ; protoplasts ; resistance ; toxicity ; Zea mays

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract The screening of 37 Zea mays L. cultivars in nutrient solution using root elongation (24 h) as a parameter showed large genotypic differences in Al resistance among the genetic material evaluated. Callose concentrations in root tips were closely and positively related to Al-induced inhibition of root elongation. Therefore, Al-induced callose formation in root tips appears to be an excellent indicator of Al injury and can be used as a selection criteria for Al sensitivity. In contrast, aluminium concentrations in root tips were not related to Al-induced inhibition of root elongation, nor to Al-induced callose formation. Callose formation was also induced by short-term A1 treatment in root tip protoplasts, and the response of protoplasts clearly reflected the cultivar-specific response to Al of intact roots. This indicates that in maize, Al sensitivity is expressed on the protoplast level.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1004204120863

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