Abstract
The screening of 37 Zea mays L. cultivars in nutrient solution using root elongation (24 h) as a parameter showed large genotypic differences in Al resistance among the genetic material evaluated.
Callose concentrations in root tips were closely and positively related to Al-induced inhibition of root elongation. Therefore, Al-induced callose formation in root tips appears to be an excellent indicator of Al injury and can be used as a selection criteria for Al sensitivity. In contrast, aluminium concentrations in root tips were not related to Al-induced inhibition of root elongation, nor to Al-induced callose formation. Callose formation was also induced by short-term A1 treatment in root tip protoplasts, and the response of protoplasts clearly reflected the cultivar-specific response to Al of intact roots. This indicates that in maize, Al sensitivity is expressed on the protoplast level.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ceballos H, Pandey S, Knapp E B and Duque J 1995 Progress from selection for tolerance to soil acidity in five tropical maize populations.InPlant-Soil lnteractions at Low pH. Eds. R A Date, NJ Grundon, GE Rayment and ME Probert. pp 419-424. Kluwer Academic Publishers, Dordrecht.
Delhaize E, Craig S, Beaton C D, Bennet R J, Jagadish V C and Randall P J 1993 Aluminium tolerance in wheat (Triticum aestivumL.) I. Uptake and distribution of aluminium in root apices. Plant Physiol. 103, 685-693.
Furlani P R and Furlani A M C 1991 Tolerância a aluminio e eficiência a fósforo em milho e arroz: características independentes. Bragantia, Carnpinas 50, 331-340.
Horst W J 1995 The role of the apoplast in aluminium toxicity and resistance of higher plants: a review. Z. Pflanzenernähr. Bodenkd. 158, 419-428.
Granados G, Pandey S and Ceballos H 1993 Response of selection for tolerance to acid soils in a tropical maize population. Crop Sci. 33, 936-940.
Köhle H, Jeblick W, Poten F, Blaschek Wand Kauss H1985 Chitosan elicited callose synthesis in soybean cells as a Ca2+-dependent process. Plant Physiol. 77, 544-551.
Lima M, Furlani P R and Miranda Filho de J B 1992 Divergent selection for aluminium tolerance in amaize (ZeamaysL.) population. Maydica 37, 123-132.
Llugany M, Massot N, Wissemeier A H, Poschenrieder Ch, Horst W J and Barceló J 1995 Aluminium tolerance of maize cultivars as assessed by callose production and root elongation. Z. Pflanzenernähr. Bodenkd. 157, 447-451.
Llugany M, Poschenrieder Ch and Barceló J 1994 Monitoring of aluminium-induced inhibition of root elongation in four maize cultivars differing in tolerance to aluminium and proton toxicity. Physiol. Plant. 93, 265-271.
Magnavaca R and Bahia Filho A F C 1993 Success in maize acid soil tolerance.InAdaptation of Plants to Soil Stress. Ed. J W Maranvill. pp 209-220. Intsormil Publ. No. 94-2. University of Nebraska, Lincoln.
Pandey S, Ceballos H, Magnavaca R, Bahia Filho A F C, Duque-Vargas J and Vinsco L E 1994 Genetics of tolerance to soil acidity in tropical maize. Crop Sci. 34, 1511-1514.
Pandey S, Ceballos H and Granados G 1995 Registration of four tropical maize populations with acid soil tolerance: SA-4, Sa-5, SA-6, and SA-7. Crop Sci. 35, 1230-1231.
Rincón M and Gonzales R A 1992 Aluminium partitioning in intact roots of aluminium-tolerant and aluminium-sensitive wheat (Triticum aestivumL.) cultivars. Plant Physiol. 99, 1021-1028.
Ryan P R, Ditomaso J M and Kochian L V 1993 Aluminium toxicity in roots: an investigation of spatial sensitivity and the role of the root cap. J. Exp. Bot 44, 437-446.
Schaeffer H J and Walton J D 1990 Aluminium ions induce oat protoplasts to produce an extracellular (1->3)β-D-glucan. Plant Physiol. 94, 13-19.
Scott B J and Fisher J A 1993 Comparing selection strategies for tolerance of acid soils.InAdaptation of Plants to Soil Stress. Ed. J W Maranville. pp 103-116. Intsormil Publ. No.94-2. University of Nebraska, Lincoln.
Staß A and Horst W J 1995 Effect of aluminium on membrane properties of soybean (Glycine max) cells in suspension culture. Plant Soil 171, 111-118.
Taylor G J 1995 Overcoming barriers to understanding the cellular basis of aluminium. Plant Soil 171, 89-103.
Wagatsuma T and Akiba R 1989 Low surface negativity of root protoplasts from aluminium-tolerant plant species. Soil Sci. Plant Nutr. 35, 443-452.
Wagatsuma T, Nakashima T and Tawaraya K 1991 Identification of aluminium-tolerant protoplasts in the original root protoplast population from several plant species differing in aluminium tolerance.InPlant-Soil Interactions at Low pH. Ed. R J Wright, V C Baligar and R P Murrmann. pp 789-793. Kluwer Academic Publishers, Dordrecht.
Wagatsuma T, Ishikawa S, Obata H, Tawaraya K and Katohda S 1995 Plasma membrane of younger and outer cells is the primary specific site for aluminium toxicity in roots. Plant Soil 171, 105- 112.
Wissemeier A H and Horst W J 1995 Effect of calcium supply on aluminium-induced callose formation, its distribution and persistence in roots of soybean (Glycine max(L.) merr.) J. Plant Physiol. 145, 470-476.
Wissemeier A H, Kotz F and Horst W J 1987 Aluminium induced callose synthesis in roots of soybean (Glycine maxL.) J. Plant Physiol. 129, 487-492.
Wissemeier A H, Diening A, Hergenröder A, Horst W J and Mix-Wagner G 1992 Callose formation as parameter for assessing genotypical plant tolerance of aluminium and manganese. Plant Soil 146, 67-75.
Zhang G, Hoddinott J and Taylor G J 1994 Characterization of 1,3 β-D-glucan (callose) synthesis in roots ofTriticum aestivumin response to aluminium toxicity. J. Plant Physiol. 144, 229-234.
Rights and permissions
About this article
Cite this article
Horst, W., Püschel, AK. & Schmohl, N. Induction of callose formation is a sensitive marker for genotypic aluminium sensitivity in maize. Plant and Soil 192, 23–30 (1997). https://doi.org/10.1023/A:1004204120863
Issue Date:
DOI: https://doi.org/10.1023/A:1004204120863