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  • 1
    Electronic Resource
    Electronic Resource
    Detection of lipopolysaccharide-binding proteins on membranes of murine lymphocyte and macrophage-like cell lines (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 76 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Lipopolysaccharide-(LPS) binding proteins present on murine-lymphocyte and macrophage-like cell lines were identified by a ligand-blotting method and subsequent immunological detection of bound LPS. Membrane proteins of the murine-pre-B-cell line 70Z/3 were separated by SDS-PAGE, transferred electrophoretically onto nitrocellulose, and the blot was incubated with LPS of the Salmonella minnesota Re-mutant R595 (mRe-LPS). LPS bound to proteins on nitrocellulose was immunologically detected by anti-mRe-LPS antibodies; LPS was associated with one of the membrane proteins of 70Z/3 cells. This protein was 40 kDa under reducing and 45 kDa under non-reducing conditions, respectively. Treatment of 70Z/3 cells with pronase led to the disappearance of the LPS-binding protein indicating its surface location. Excess free lipid A, which represents the biologically active region of LPS, inhibited the binding of mRe-LPS to the protein. This LPS-binding protein was also identified on the pre-B-cell line CYG8, the B-cell line CYG101 and the murine-T-cell line BW5147. It was, however, not detectable on the B-cell line CYG34 and the myeloma-cell line P3-X63-Ag8.653. No other LPS-binding protein could be detected on these cell lines. In the murine-macrophage-like cell line J774.1, two LPS-binding proteins, one of 40 kDa and one of 80 kDa, were detected. These results indicate that mRe-LPS is specifically bound to a 40-kDa protein of lymphocytes, whereas in the case of macrophages it is associated with two LPS-binding proteins of 40 and 80 kDa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04257.x
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  • 2
    Electronic Resource
    Electronic Resource
    Maintenance of visual cells in vitro (1960)
    Springer
    Cellular and molecular life sciences 16 (1960), S. 363-364 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Des fragments de rétine et des cellules visuelles isolées de la grenouilleRana pipiens ont été cultivés dans la chambre de Rose. Bien qu'une dégénérescence morphologique des segments extérieurs des cellules visuelles ait été observée pendant la culture, le pouvoir de réfraction normal et la présence du pigment visuel ont été constatés dans ces segments, les segments intérieurs avec leurs noyaux étant conservés. Mais, l'intensité du pigment visuel a été plus faible que dans la rétine fraîche. Ces résultats montrent que la cellule individuelle du photorécepteur se prête à l'étude physiologiquein vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02157906
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic diversity of plums characterized by random amplified polymorphic DNA (RAPD) analysis (1999)
    Springer
    Euphytica 109 (1999), S. 143-147 
    Details
    ISSN: 1573-5060
    Keywords: classification ; DNA ; plum varieties ; Prunus ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We investigated the genetic diversity of 42 plum varieties by RAPD analysis. Twenty primers discriminated all plum varieties excepting two synonymous pairs: 'Botankyou and ‘Kelsey’, and ‘Chairn’ and ‘Tragedy’. Two North American plums, ‘Beach Plum’ and ‘Glow’, were genetically distinct from the other plums by cluster analysis. Overlaps observed between the ‘European plum group’ and the ‘Japanese plum group’, were perhaps due to intercrossing. We could also discriminate ‘Sordum’ from 'Late Sordum and ‘Bansei Sordum’, although ‘Late Sordum’ and ‘Bansei Sordum’ are thought to be derived from bud mutants of ‘Sordum’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003728201100
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  • 4
    Electronic Resource
    Electronic Resource
    Distribution and development of the serotonin-and RFamide-like immunoreactive neurons in the arrowworm, Paraspadella gotoi (Chaetognatha) (1992)
    Springer
    Cell & tissue research 267 (1992), S. 215-222 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Serotonin ; RFamide ; Development ; Paraspadella gotoi (Chaetognatha)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution and development of serotonin-and RFamide-like immunoreactivities in the nervous system of Chaetognatha, Paraspadella gotoi, were examined in whole-mount preparations. In adults, a single serotonin-like immunoreactive (5HTLI) neuron and numerous RFamide-like immunoreactive (RFaLI) neurons were found in the central nervous system. Based on the structure of the fins, hooks, and eyes, seven postembryonic developmental stages were recognized. The most obvious features of the stages are: stage 1, newly hatched young; stage 2, elongation of a continuous lateral tail fin; stage 3, separation of the lateral and tail fins; stage 4, appearance of hooks; stage 5, pigmentation of eyes, stage 6, attachment by tail adhesive fins; stage 7, prey capture. Stage 1 did not show any immunoreactivity. The 5HTLI neuron first appeared at stage 4 and its axonal pathway became similar to the adult at stage 6. On the other hand, the RFaLI neurons appeared at stage 3 in the ventral ganglion. Some of their somata disappeared at stage 5 and the neuronal architecture resembled the adult at stage 7 although the RFaLI neurons in the cerebral ganglion were complete at the juvenile stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00302958
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  • 5
    Electronic Resource
    Electronic Resource
    A unique photoreceptive structure in the arrowworms Sagitta crassa and Spadella schizoptera (Chaetognatha) (1984)
    Springer
    Cell & tissue research 235 (1984), S. 471-478 
    Details
    ISSN: 1432-0878
    Keywords: Photoreceptive structure ; Arrowworm ; Perforated lamellae ; Chaetognatha
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of photoreceptors in two species of chaetognaths, Sagitta crassa and Spadella schizoptera, was studied by electron microscopy using a goniometer specimen stage as well as by freeze-fracture techniques. In contrast to earlier descriptions, the photoreceptor is made up of a stack of lamellae with pores. The lamellae, each 30–45 nm in thickness, are piled one on top of one another at intervals of 10–20 nm. The lamellar surface is often perpendicular to the incident light. The pores are 35–55 nm in diameter and arranged in an orderly square lattice with a center-to-center distance of 80–95 nm. These perforated lamellar structures, resembling annulate lamellae, are entirely new as photoreceptive structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226941
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  • 6
    Electronic Resource
    Electronic Resource
    Synthesis of Diospyrin, a Potential Agent Against Leishmaniasis and Related Parasitic Protozoan Diseases (2000)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 2000 (2000), S. 1313-1317 
    Details
    ISSN: 1434-193X
    Keywords: Leishmaniasis ; Natural products ; Phytochemistry ; Quinones ; C-C coupling ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: -The first synthesis of diospyrin [2,6′-bis(5-hydroxy-7-methyl-1,4-naphthoquinone), 1] was achieved by employing Suzuki coupling between 5 and 14 as the key reaction to connect the two 7-methyljuglone units.
    Type of Medium: Electronic Resource
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  • 7
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    Origin of transient self-potential signals associated with very-long-period seismic pulses observed during the 2000 activity of Miyakejima volcano (2015)
    Wiley
    Details
    Publication Date: 2015-04-24
    Description: Origin of the previously reported transient geoelectrical (Self-Potential, SP) signals in the Miyakejima 2000 activity, that repeatedly occurred concurrently with Very-Long-Period (VLP) seismic pulses, was investigated. SP waveforms stacked across repeated VLP events showed a step-like rise followed by a gradual decay at all stations spread over the island of 8-km diameter. Within a realistic range of hydrological diffusivity, the short time constants of the SP signals cannot be explained by the electrokinetic effect caused by fluid flow within a limited volume, proposed earlier as a fluid injection hypothesis. On the other hand, poroelasticity predicts an island-wide distributed flow field to occur almost instantaneously upon VLP events due to the step of strain field imposed by the mechanical event. We propose that the observed SP signals resulted from the streaming current by this island-wide flow field. Our quantitative model, assuming a vertical tensile crack as a mechanical source, which has been suggested by preceding seismic studies, can explain the time constants and the amplitude of the SP signals (both spatial pattern and absolute amplitude), within a reasonable range of rock properties and the scalar moment of the mechanical source (VLP event). Location and attitude of the mechanical source were well-constrained by grid search, and is consistent with those estimated earlier from other types of data.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
    Published by Wiley on behalf of American Geophysical Union (AGU).
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  • 8
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    Distribution and development of the serotonin-and RFamide-like immunoreactive neurons in the arrowworm, Paraspadella gotoi (Chaetognatha) (1992)
    Springer
    Details
    Publication Date: 1992-02-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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  • 9
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    A unique photoreceptive structure in the arrowworms Sagitta crassa and Spadella schizoptera (Chaetognatha) (1984)
    Springer
    Details
    Publication Date: 1984-03-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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  • 10
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    Recovery of plate coupling at a ruptured asperity (2013)
    Wiley
    Details
    Publication Date: 2013-04-04
    Description: [1]  Earlier earthquake cycle simulations using rate- and state-dependent friction (RSF) law have revealed that frictional coupling at a ruptured asperity starts immediately after dynamic slip, because of logarithmically time-dependent healing. However, some GPS inversion studies of the 2011 great Tohoku-oki earthquake suggest that after slip continued for 7–8 months or longer in the deep seismogenic interface including asperities where M7 earthquakes repeatedly occurred. Nakatani and Scholz [2006] introduced the intrinsic cutoff time t cx in the logarithmically time dependent healing and pointed out that a long t cx could lead to delayed start of frictional coupling. Assuming RSF laws into which t cx was incorporated, we conducted a numerical simulation using a block spring model. We defined the time required to restore frictional coupling as T cpl measured from the instant of dynamic slip. The value of T cpl for t cx  = 10 -3  s was almost the same as T cpl in the conventional law, which was on the order of 10 minutes in the case of the aging law. It increased approximately in proportion to t cx for short t cx (≤1 s), whereas T cpl was not so sensitive to t cx for a long t cx (〉1 s). Moreover, a long t cx cannot be reasonably assumed because the maximum slip velocity during dynamic slip decreased with increasing t cx , limiting the largest possible T cpl to be months at most. Friction laws with two state variables, each having a different cutoff time, were also investigated. The maximum slip velocity was determined by the smaller cutoff time, and T cpl was affected by the larger cutoff time. As a result, longer T cpl became possible, although the values are still on time scale of months.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
    Published by Wiley on behalf of American Geophysical Union (AGU).
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