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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 86 (1998), S. 175-182 
    ISSN: 1570-7458
    Keywords: Aleyrodidae ; greenhouse whitefly ; nitrogen ; host selection ; oviposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tomato plants, Lycopersicon esculentum Miller cv 'Tres Cantos', were supplied with high (308 ppm), medium (140 ppm) and low (84 ppm) nitrogen doses in order to determine the influence of nitrogen fertilization on feeding and oviposition site selection by greenhouse whitefly adults, T. vaporariorum (Westwood). The nitrogen and water content was higher in plants supplied with 308 N ppm than in plants with medium or low nitrogen doses and no differences were found in soluble sugar content. The leaves of the upper plant stratum (leaves 1–5) had the greatest nitrogen and water content, independent of the nitrogen dose applied. The plants were exposed to T. vaporariorum adults into a greenhouse under two current (late autumn-winter and spring-early summer) situations of the tomato crop in the Mediterranean area. The distribution of whitefly adults on the plants was affected by the nitrogen dose in both experimental conditions, the number of adults was higher on the plants supplied with high nitrogen dose. Within plants, adult distribution was affected by leaf position, the upper plant stratum being preferred for feeding. Whitefly females selected for oviposition and laid more eggs on plants and leaves of plants with higher nitrogen and water contents. We conclude that whitefly host selection for feeding and ovipositing are related to differences in nitrogen and water content of the host plant.
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  • 2
    ISSN: 1570-7458
    Keywords: fecundity ; host plant quality ; Pistia stratiotes ; Spodoptera pectinicornis ; nitrogen ; trichomes ; oviposition ; biological control of weeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We determined the influence of larval host quality of the floating aquatic weed Pistia stratiotes L. (Araceae) on the fecundity and egg distribution of the biological control agent Spodoptera pectinicornis (Hampson) (Lepidoptera: Noctuidae). Different nutritional levels were produced by growing plants with relatively low and high levels of nitrogen, phosphorus and potassium fertilizer. Female and male pupal biomass were significantly greater when the larvae were fed leaves from the high fertilizer plants. Although the fertilizer treatments did not significantly influence total fecundity, there was an indirect effect as adults from larger pupae were more fecund. Regardless of treatment, ovipositing females formed a depression in the leaf surface by removing trichomes into which they deposited the egg masses. Most of the egg masses were laid on the lower leaf surface, on leaf positions 5–8 (counting from the young inner to the outer leaves) and during days 1–2 post-eclosion. Most of the eggs were laid in masses but about 12% were solitary. Females fed the low fertilizer treatment laid a greater proportion (mean ± s.e.) of their eggs as solitary eggs (17.3 ± 3.4% of total eggs) than did females fed the high fertilizer treatment (8.3 ± 2.3% of total eggs). The increased percentage of solitary eggs laid by the females from the low quality larval diet may be an adaptive response to decrease competition among the progeny.
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  • 3
    ISSN: 1570-7458
    Keywords: Bemisia argentifolii ; Encarsia formosa ; poinsettia ; parasitoids ; nitrogen ; tri-trophic level interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract More wasps of Encarsia formosa Gahan (Hymenoptera: Aphelinidae) were found on fertilized poinsettias, Euphorbia pulcherrima (Willd.) (Euphorbiaceae), than on non-fertilized plants. Parasitization of Bemisia argentifolii Bellows & Perring (Homoptera: Aleyrodidae) by E. formosa was higher on plants treated with calcium nitrate than with ammonium nitrate or on control plants. In a no-choice test, host feeding by E. formosa was higher when hosts were on fertilized plants than when hosts were on control plants. The nitrogen content of whitefly pupae reared on plants treated with ammonium nitrate was higher than those on calcium nitrate-treated plants. Variability in the parasitization of B. argentifolii by E. formosa appears to be due to host plant-mediated differences in the whiteflies. E. formosa may be influenced by the nutritional suitability of the host, which influences whether wasps continue to oviposit, feed, or disperse.
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  • 4
    ISSN: 1570-7458
    Keywords: Brassica rapa ; B. juncea ; consumption rate ; glucosinolates ; growth rate ; indolyl ; isothiocyanate ; Mamestra configurata ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mamestra configurata (Walker) (Lep., Noctuidae) larvae were fed excisedBrassica juncea (commercial brown mustard) orB. rapa cv. Tobin (Canola) foliage of three plant growth stages-rosette (stage 2), stem elongation (stage 3) and flowering (stage 4). Relative consumption rates (RCRi) were not significantly different between the plant species. Within theB. juncea treatments, there were no significant growth stage differences in RCRi. However, withinB. rapa, RCRi increased with advancing plant growth stage. Larvae fedB. juncea foliage had significantly reduced relative growth rates (RGRi) compared to larvae fedB. rapa foliage. Within theB. juncea treatments, RGRi decreased with advancing plant growth stage. There were no significant growth stage differences in RGRi in theB. rapa treatments. RGRi was inversely proportional to the levels of isothiocyanate-releasing glucosinolates in theB. juncea treatments. RCRi was inversely proportional to the levels of indolyl glucosinolates in theB. rapa treatments. Levels of total phenols and catechols inB. juncea did not show any trend which could be related to growth stage effects in the insect nutritional indices. InB. rapa, levels of phenols and catechols in stage 3 and 4 foliage were lower than that of stage 2 foliage. Analyses of total nitrogen in field-grown plants showed reductions in percent nitrogen from rosette to flowering stage foliage. The response ofM. configurata to different growth stages of its host plants are discussed in relation to changing levels of allelochemicals and nitrogen.
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  • 5
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    Entomologia experimentalis et applicata 92 (1999), S. 205-216 
    ISSN: 1570-7458
    Keywords: Samea multiplicalis ; Spodoptera pectinicornis ; Pistia stratiotes ; waterlettuce ; nitrogen utilization efficiency ; compensatory feeding ; nitrogen ; biological control of weeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Compensatory responses of caterpillars fed low quality food include increased consumption and utilization of essential nutrients. Information about an insect's responses to nutritional challenges from their host plants could benefit weed biological control efforts in the selection and establishment of new agents. The target weed, Pistia stratiotes L. (Araceae) is a floating aquatic plant that has relatively low nitrogen levels which are further diluted with high water content. Efforts to establish the insect Spodoptera pectinicornis (Hampson) (Lepidoptera: Noctuidae) for biological control of P. stratiotes could benefit by examining the nutritional responses of a similar widely established lepidopteran species, Samea multiplicalis (Guenèe) (Lepidoptera: Pyralidae). Larvae of this species were fed leaves of P. stratiotes plants that had been fertilized (NPK) at high and low rates. The leaves of the fertilized plants had a 4.3-fold increase in nitrogen (dry weight) and a 1.6-fold increase in water content. The results suggest that no compensatory increases occurred in larvae fed leaves from the low fertilized plants as no changes were found in fresh mass consumption or nitrogen utilization efficiency. Consequently, development time from second-third instars to pupation was delayed about 3 days compared with larvae fed the high nitrogen leaves. Furthermore, consumption of nitrogen was only 30% and its accumulation into larval tissues was only 60% compared with the larvae fed the high fertilized leaves. The resulting larvae had both a final biomass and a growth rate that were reduced by 40%. Regardless of plant fertilizer level, the larvae fed at a rate 5–10 times greater than that of similar lepidopteran species consuming either low or high quality diets, suggesting that the S. multiplicalis larvae may be functioning at their biological limit for ingesting food.
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  • 6
    ISSN: 1570-7458
    Keywords: Coleoptera ; bruchidae ; anesthesia ; carbon dioxide ; nitrogen ; cold ; oviposition and mating
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of anesthetizing with carbon dioxide, nitrogen, or low temperature on the reproductive behavior and fecundity of Callosobruchus subinnotatus was investigated. Males and females anesthetized with N2 as virgins had shorter recovery time, copulated earlier, and had shorter mating time than those subjected to CO2 or low temperature. Exposure of males and females to 2.0±0.5 °C for 10 min had the most profound effect on the recovery periods, copulation time, and duration of mating. Adults anesthetized with CO2 and low temperature laid fewer eggs than those anesthetized with N2. Females were more affected than males by the treatments, except when males were chilled for 10 min, and this prolonged the time for commencement of mating. The mode of anesthesia could affect egg production because vitellogenesis and choriogenesis take place in the imago stage of bruchids.
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  • 7
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    Entomologia experimentalis et applicata 77 (1995), S. 205-210 
    ISSN: 1570-7458
    Keywords: nitrogen ; maize ; lepidopterous stem and cob borers ; Sesamia calamistis ; Eldana saccharina ; Mussidia nigrivenella ; Cryptophlebia leucotreta ; dead hearts ; stem tunneling ; yields
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of four nitrogen levels (0, 60, 90 and 120 kg N/ha) on growth of maize and development of lepidopterous pests was investigatdd in a field trial. Nitrogen had a positive effect on both plant growth variables (plant height, stem diameter and yield), and development and survival ofSesamia calamistis andEldana saccharina, and thereby increased the incidence of dead hearts and stem tunneling. However, the percent yield loss due to artificial infestation decreased with increasing N application rate from 20% to 11% in the in the 0kg/ha and 120kg/ha treatment, respectively. Using a multiple regression analysis, plant height, plant diameter and stem tunneling were found to be the most important variables explaining the variability in maize yield.
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  • 8
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    Entomologia experimentalis et applicata 92 (1999), S. 165-177 
    ISSN: 1570-7458
    Keywords: Chrysomelidae ; herbivory ; Asteraceae ; life history ; nitrogen ; plant quality ; season
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytophagous insects which feed on the leaves of herbaceous host plants have to adapt their life histories to the fact that protein nitrogen is usually highest in growing tissues in spring. We monitored field populations of larvae and adults of three chrysomelid species (Galeruca tanaceti (L.) (main host Achillea millefolium (L.) Yarrow), Cassida rubiginosa (Mueller) (main host Cirsium arvense (L.) Scop.) and Oreina luctuosa (Suffrian) (host Centaurea scabiosa (L.)) together with the amount of protein nitrogen of their food resources and host plant biomass. As expected, the development of host quality, measured as concentration of protein nitrogen, and host plant biomass showed inverse trends during the season. The euryphagous G. tanaceti attacks Achillea early and profits from high nitrogen concentrations in the leaves. Occasional overexploitations of local populations of Achillea are compensated by the capacity to move to other host species. In C. rubiginosa, a species with a host range restricted to the Cardueae, the main larval feeding activity is postponed to a period when the nitrogen content of the host leaves had dropped to 50% of its initial value, but when host plant biomass had increased by 30%. In the monophagous O. luctuosa the larval development is synchronized with a still later phase of host phenology, at which the nitrogen content is below 50% but plant biomass has reached its maximum. There seem to be selection factors, which oppose the use of high quality food in spring and which force the latter two species to postpone their larval development to a later time in the year. This could be caused by numerous factors like, for example, mean daytime temperature. Later in the season the larvae have to cope with the low quality of their host plants. They have, however, the advantage of large quantities of food available. A laboratory study with adults and mature larvae of O. luctuosa shows that this species can overcome low levels of protein nitrogen either by selecting younger leaves with higher nitrogen concentrations or by increasing the daily food consumption rate (RCR) on leaves with a low level of nitrogen and by a prolongation of the feeding period. In this way the larvae compensate the effect of lower daily growth rates (RGR) and a lower food conversion index (ECI) on poor food quality: Regardless of the level of protein nitrogen there was no statistically significant difference in total gain of weight during the third-instar feeding period and in the weight at the end of the third larval stage. The three investigated chrysomelids show that there exists a broad spectrum of adaptations to overcome the dilemma of variable food quality.
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  • 9
    ISSN: 1572-8927
    Keywords: Supersaturation ; nitrogen ; cosolvent ; cetyltrimethylammonium bromide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The decomposition of ammonium nitrite in water creates a supersaturated solution of nitrogen. The same process occurs in water-organic solvent mixtures. Acetone, dioxane, dimethylsulfoxide (DMSO) and dimethylformamide (DMF) are the cosolvents used in this study. The limits of supersaturation of nitrogen (C SL /mol L−1) were determined in all of these solvent mixtures by releasing the dissolved gas sonicationally and measuring the volume of released gas. C SL was generally increased in the presence of cosolvents. The effectiveness sequence of organic solvents was found to be as DMF〈DMSO〈Dioxanet≅Acetone. Transportation period of small bubbles formed during sonication is changed by compositions of solvent mixtures. These periods may depend on the viscosity of the solution. Effects of the presence of cetyltrimethylammonium bromide were also studied. It was concluded that there may be a relation between the strength of the watercosolvent H-bonds and C SL and all of the measured quantities of this study were generally affected by micelle formation.
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  • 10
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    Journal of insect behavior 11 (1998), S. 179-189 
    ISSN: 1572-8889
    Keywords: open-air foraging ; caste ; polyethism ; litter feeder ; nitrogen ; termite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Southeast Asian nasute termite Longipeditermes longipes forages on the open ground on leaf litter. Its monomorphic workers carry back food balls in their mandibles while dimorphic soldiers defensively surround the marching columns and the foraging patches. When mechanically disturbed, workers hide under the litter, whereas antennating soldiers face as closely as possible the source of disturbance. Foragers prefer the lower, nitrogen-rich layer of the litter. Soldier behavior and column organization (returning workers in the center lanes, outgoing workers in the two flanking lanes) are similar to those in the related genera Hospitalitermes and Lacessititermes, which, however, tend to forage above ground.
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  • 11
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    Journal of solution chemistry 13 (1984), S. 335-348 
    ISSN: 1572-8927
    Keywords: Solubility ; Henry's law coefficients ; Ostwald coefficients ; aqueous solutions ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The solubility of nitrogen in pure liquid water was measured in the pressure range 45 to 115 kPa and in the temperature range 5 to 50°C. These data are used to obtain Henry coefficients H 2,1 (T,P s,1 ) at the vapor pressure P s,1 of water. The temperature dependence of H 2,1 (T,P s,1 ) is accounted for by both a Clarke-Glew (CG) type fitting equation, and a power series in T−1, as suggested by Benson and Krause (BK). The imprecision of our measurements is characterized by an average deviation of ±0.038% from a four-term CG equation, and by an average deviation of ±0.042% from a three-term BK equation. From the temperature variation of H 2,1 (T,P s,1 ) partial molar quantities referring to the solution process, such as enthalpies and heat capacities of solution, are obtained. They are given in tabular form, together with H 2,1 (T,P s,1 ) and derived Ostwald coefficients L∞, at rounded temperatures. Finally, experimental results are compared with values calculated via scaled particle theory.
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  • 12
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    Catalysis letters 37 (1996), S. 163-165 
    ISSN: 1572-879X
    Keywords: nitrogen ; Rh(111) ; thermal desorption ; Arrhenius parameters ; lateral interactions ; NO decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Associative desorption of N atoms from the Rh(111) surface is simulated in the framework of the lattice-gas model. The Arrhenius parameters and nearest-neighbour lateral interaction employed to describe the measured thermal desorption spectra are as follows:v=1013 s−1,E d=40 kcal/mol, and ε1=1.7 kcal/mol. The results obtained are used to clarify the role of nitrogen desorption in the NO + CO reaction on Rh(111) atT=400–700 K andP NO≈P CO≈0.01 atm.
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  • 13
    ISSN: 1570-7458
    Keywords: Alsophila pometaria ; Geometridae ; Anisota senatoria ; Citheroniidae ; Quercus ; nutritional ecology ; herbivory ; nutritional indices ; consumption ; growth ; utilization efficiency ; nitrogen ; water ; tannins ; phenols ; gut pH ; digestive enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Alimentées sur feuillage jeune de chêne, les chenilles d'Alsophila pometaria avaient un taux relatif de croissance (RGR) et un taux relatif d'accumulation d'azote (RNAR) plus élevés que les chenilles d'Anisota senatoria alimentées sur feuillage mûr de chêne. Bien que le jeune feuillage soit plus efficacement digéré par A. pometaria (AD plus élevé), il n'est pas assimilé et utilisé pour la croissance avec de meilleurs rendements (les ECI ne sont pas différents). Ainsi le taux de croissance plus élevé d'A. pometaria est dû entièrement à un taux de consommation plus important (RCR et RNCR). Le feuillage jeune est significativement plus riche en azote et en eau que le feuillage mûr, mais les niveaux de phénol et de tanins sont les mêmes. A pometaria consomme les feuilles de différentes espèces de chênes au même taux, indépendamment de la teneur en azote, tandis que A. senatoria accroît sa consommation en réponse à une diminution de la teneur en azote. Il en résulte que le taux de croissance d'A. pometaria dépend directement de la teneur en azote des feuilles, tandis que celui d'A. senatoria en est indépendant. Les systèmes digestifs des deux insectes sont biochimiquement semblables et sont efficaces pour la digestion des protéines. Les tanins et les phénols n'influent pas sur les indices nutritionnels de ces deux espèces. Nous estimons que le principal intérêt de l'alimentation printanière est la disponibilité en feuillage succulent, riche en azote, et non l'absence de feuilles à haute teneur en tanin. L'alimentation printanière semble correspondre à une strategie alimentaire qui favorise la croissance aux dépens de l'efficacité tandis que l'alimentation en fin d'été est une stratégie qui favorise l'efficacité sur la rapidité.
    Notes: Abstract The larvae of Alsophila pometaria (Harr.), feeding on the young foliage of oak, has a higher relative growth rate (RGR) and relative nitrogen accumulation rate (RNAR) than the larvae of Anisota senatoria (J. E. Smith), feeding on the mature foliage of oak. Although the young oak foliage is more efficiently digested by A. pometaria (higher AD's), it is not more efficiently assimilated and used for growth (no difference in ECI's). Thus, the higher growth rate of A. pometaria is due entirely to a higher consumption rate (RCR and RNCR). Young foliage is significantly higher in nitrogen and water than mature foliage, but phenol and tannin levels are comparable in young and old foliage. A. pometaria consumes the foliage of different oak species at the same rate, independent of nitrogen content, while A. senatoria increases its consumption rate in response to decreased nitrogen levels. As a result, the growth rate of A. pometaria is directly related to leaf nitrogen content, while the growth rate of A. senatoria is independent of leaf nitrogen. The two species of insects have digestive systems that are very similar biochemically, and that are well-designed for effective protein digestion. Tannins and phenols do not influence the nutrional indices of either species. We suggest that the major benefit of spring feeding is the availability of succulent, high-nitrogen foliage, and not the avoidance of high-tannin foliage. The spring feeder appears to have a feeding strategy that favors rapid growth at the expense of efficiency, while the late summer feeder has a strategy that favors efficiency over rate.
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  • 14
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    Entomologia experimentalis et applicata 36 (1984), S. 293-295 
    ISSN: 1570-7458
    Keywords: ryegrass ; Oscinella frit ; Geomyza tripunctata ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 15
    ISSN: 1572-879X
    Keywords: adsorption ; adsorption isotherms ; dinitrogen ; FTIR spectroscopy ; geminal species ; NaY ; nitrogen ; zeolites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Adsorption of N2 on NaY zeolites at 85 K and equilibrium pressures higher than 1 kPa results in the formation of geminal dinitrogen complexes characterized by an IR band at 2333.5 cm−1 (2255.4 cm−1 after adsorption of 15N2). With decreasing equilibrium pressure the complexes tend to loose one N2 ligand, thus forming linear species characterized by an IR band at 2336.8 cm−1 (2258.7 cm−1 after adsorption of 15N2). All species disappear completely after evacuation. Co-adsorption of N2 and CO revealed that the dinitrogen complexes are formed on Na+ cations. The changes in the concentrations of the linear and geminal N2 species with the changes in the equilibrium pressure are excellently described by equations of adsorption isotherms proposed earlier for mono- and di-carbonyls.
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  • 16
    ISSN: 1572-8757
    Keywords: kinetics ; isotope-exchange ; nitrogen ; adsorption ; methane ; zeolite ; equilibria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Physics , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The Isotope Exchange Technique (IET) was used to simultaneously measure pure and binary gas adsorption equilibria and kinetics (self-diffusivities) of CH4 and N2 on pelletized 4A zeolite. The experiment was carried out isothermally without disturbing the adsorbed phase. CH4 was selectively adsorbed over N2 by the zeolite because of its higher polarizability. The multi-site Langmuir model described the pure gas and binary adsorption equilibria fairly well at three different temperatures. The selectivity of adsorption of CH4 over N2 increased with increasing pressure at constant gas phase composition and temperature. This curious behavior was caused by the differences in the sizes of the adsorbates. The diffusion of CH4 and N2 into the zeolite was an activated process and the Fickian diffusion model described the uptake of both pure gases and their mixtures. The self-diffusivity of N2 was an order of magnitude larger than that for CH4. The pure gas self-diffusivities for both components were constants over a large range of surface coverages (0 〈 θ 〈 0.5). The self-diffusivities of CH4 and N2 from their binary mixtures were not affected by the presence of each other, compared to their pure gas self-diffusivities at identical surface coverages.
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  • 17
    ISSN: 1572-879X
    Keywords: platinum ; rhodium ; alloy ; surface composition ; nitrogen ; oxygen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Two kinds of adsorbed N atoms exist on a Pt0.25Rh0.75(100) surface. One desorbs at 490 K and the other desorbs at 650 K. The former reacts with H2 at 400 K, but the latter does not. It is supposed that the adsorption of these two N atoms is responsible of the surface composition, ratio of Pt and Rh.
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  • 18
    ISSN: 1432-0789
    Keywords: Key words Microbial biomass C ; Water-soluble organic carbon ; Light fraction organic carbon ; Fertilizer ; nitrogen ; 13C nuclear magnetic resonance ; Infrared spectrophotometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil samples taken from four experimental sites that had been cropped to continuous corn for 3–11 years in Ontario and Quebec were analyzed to evaluate changes in quantity and quality of labile soil organic carbon under different nitrogen (N) fertility and tillage treatments. Addition of fertilizer N above soil test recommendations tended to decrease amounts of water-soluble organic carbon (WSOC) and microbial biomass carbon (MBC). The quality of the WSOC was characterized by 13C nuclear magnetic resonance and infrared spectrophotometry and the results indicated that carbohydrates, long-chain aliphatics and proteins were the major components of all extracts. Similar types of C were present in all of the soils, but an influence of management was evident. The quantity of soil MBC was positively related to the quantities of WSOC, carbohydrate C, and organic C, and negatively related to quantities of long-chain aliphatic C in the soil. The quantity of WSOC was positively related to the quantities of protein C, carbohydrate C, and negatively related to the quantity of carboxylic C. The quantity of soil MBC was not only related to quantities of soil WSOC but also to the quality of soil WSOC.
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  • 19
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    Biology and fertility of soils 26 (1998), S. 169-172 
    ISSN: 1432-0789
    Keywords: Key words Urea ; Coated fertilizers ; Ammonium ; nitrogen ; Nitrate nitrogen ; Nitrogen uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Understanding the fate of different forms of nitrogen (N) fertilizers applied to soils is an important step in enhancing N use efficiency and minimizing N losses. The growth and N uptake of two citrus rootstocks, Swingle citrumelo (SC), and Cleopatra mandarin (CM), seedlings were evaluated in a pot experiment using a Candler fine sand (hyperthermic, uncoated, Typic Quartzipsamments) without N application or with 400 mg N kg–1 applied as urea or controlled-release fertilizers (CRF; either as Meister, Osmocote, or Poly-S). Meister and Osmocote are polyolefin resin-coated urea with longevity of N release for 270 days (at 25°C). Poly-S is a polymer and sulfur-coated urea with release duration considerably shorter than that of either Meister or Osmocote. The concentrations of 2 M KCl extractable nitrate nitrogen (NO3 –-N) and ammonium nitrogen (NH4 +-N) in the soil sampled 180 days and 300 days after planting were greater in the soil with SC than with CM rootstock seedlings. In most cases, the extractable NH4 + and NO3 – concentrations were greater for the Osmocote treatment compared to the other N sources. For the SC rootstock seedlings, dry weight was greater with Meister or Poly-S compared with either Osmocote or urea. At the end of the experiment, ranking of the various N sources, with respect to total N uptake by the seedlings, was: Meister = Osmocote 〉 Poly-S 〉 Urea 〉 no N for CM rootstock, and Meister = Poly-S = Osmocote 〉 Urea 〉 no N for SC rootstock. The study demonstrated that for a given rate of N application the total N uptake by seedlings was greater for the CRF compared to urea treatment. This suggests that various N losses were lower from the CRF source as compared to those from soluble fertilizers.
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  • 20
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    Biology and fertility of soils 26 (1997), S. 31-34 
    ISSN: 1432-0789
    Keywords: Key words Microbial biomass ; Air-drying ; Extractable ; nitrogen ; Extractable phosphorus ; Tropical soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The microbial contribution to extractable N and P after the air-drying of eight Indian dry tropical Ultisols was quantified. Air-drying of the soils decreased microbial biomass C by 25–53% but increased extractable N and P by 14–34% and 24–121%, respectively. This increase in the extractable N and P was accounted for, to some extent, by microbial biomass killed due to air-drying. Microbial biomass contributes 17–36% and 19–82% to the extractable N and P, respectively, possibly due to air-drying of the soils. I conclude that due to contamination of microbial biomass with the available nutrients in air-dried soils, measurements of extractable nutrients should be made on field-moist soils.
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  • 21
    ISSN: 1436-5073
    Keywords: AES ; laser ; iron ; nitrogen ; oxygen
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The laser induced modification of iron surfaces with atmospheric species was investigated by means of Auger electron spectroscopy (AES) and scanning electron microscopy (SEM). Different laser systems were used for irradiating iron samples in a wide range of the laser processing parameters up to small foci and ultra short pulses. A nitriding of iron connected with an oxidation of the near surface region was observed in the wavelength range between 193 nm and 10.6 μm using large foci (∼0.1 cm2) and short pulses (10...1400ns). In case of small foci (∼7·10−6cm2) with ns-pulses (50 ns) an enrichment of the iron melt with nitrogen and an advanced oxidation of the surrounding area of the laser spot were detected. When using shorter pulses (200 fs, 40 ps) no indications for a nitriding were found.
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  • 22
    ISSN: 1573-0832
    Keywords: ELISA ; Endodermis ; H. annosum ; Immunocytochemistry ; Root rot ; Vascular tissues
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    Topics: Biology , Medicine
    Notes: Abstract Vascular disintegration mainly of medulla rays of spruce roots is of major significance in root rot disease of spruce caused byH. annosum. Using seedling roots as an experimental model, the possible routes and initial host reactions preceding invasion of vascular tissues was investigated. Transmission electron microscopy showed that penetration through the endodermis was an obvious route but not without host resistance. Using antibodies againstH. annosum hyphal materials, some labelling of vascular tissues remote from sites of fungal colonization suggest the release of fungal secretory products partly active in tissue disintegration. Similarly, intense labelling was also observed in severely colonized host tissues at late stages of infection. Strong labelling recorded at 3 d p.i. mainly on fungal hyphae and scant gold particles on invaded host tissues could imply that induction of host antifungal metabolites may have been a late event. A correlation was found between total antigenic material in root homogenates measured by ELISA, density of tissue labelling by immunocytochemistry and severity of disease symptoms. The importance of this in relation to diagnosis of biotic root rot diseases in the field is discussed.
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  • 23
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    Nutrient cycling in agroecosystems 42 (1995), S. 1-11 
    ISSN: 1573-0867
    Keywords: nitrogen ; 15N humic substances ; acid hydrolysis ; analytical pyrolysis ; NMR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Most of the N in surface soils occurs in organic forms. The organic N in soil plays a key role in plant nutrition and soil fertility through its effects on microbial activity and nutrient availability. Typically, about one-third of the fertilizer N applied to temperate-zone soils is immobilized and retained in organic forms at the end of the growing season. A significant portion of this newly immobilized N is no more available to microorganisms and plants than the native humus N. Stabilization processes, probably involving polymerization of amino compounds and polyphenols, result in incorporation of N into humic substances with a concurrent reduction in N availability. This paper presents an account of the forms and nature of organic N in soil, emphasizing possible formation pathways, chemical characterization of humic substances through conventional and solid-state techniques, and the fate and composition of newly immobilized N in soil.
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  • 24
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    Nutrient cycling in agroecosystems 42 (1995), S. 139-148 
    ISSN: 1573-0867
    Keywords: denitrification ; methods ; 15N ; nitrate ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper attempts to answer the question: is denitrification a major route of N loss from tropical agro-ecosystems? This question turns out to be very difficult to answer due to a severe shortage of data on this process for tropical agro-ecosystems other than rice. Given this lack of data, I approach this question by analyzing data on denitrification and nitrous oxide flux in tropical native forest and pasture soils and attempt to make some conclusions and pose some hypotheses about the significance of denitrification in tropical agricultural soils. I also briefly review methods for measuring denitrification. The data analysis suggests that denitrification in tropical forest soils is strongly influenced by the nature and amount of soil C and N turnover. Studies to examine differences in denitrification in different tropical agricultural systems should focus on the effects of system management on C and N turnover. The data analysis also suggests that, just as in temperate regions, denitrification may not be a significant route of N loss from most tropical agricultural systems. However, field studies are necessary to determine if this is actually the case.
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  • 25
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    Nutrient cycling in agroecosystems 45 (1995), S. 221-233 
    ISSN: 1573-0867
    Keywords: fertilizer recovery ; modelling ; nitrogen ; nutrient efficiency ; nutrient surplus ; phosphorus ; Poland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Research on nutrient losses from agricultural systems should try to relate these losses to farm characteristics. This was done for private farms in two districts in Poland. Using data from a farm survey, nutrient surpluses and Nutrient Use Efficiency (NUE, defined as the ratio of outgoing and incoming nutrients) were calculated for nitrogen and phosphorus. Both nutrient surplus and NUE are relatively high. A model was developed to estimate surpluses and NUE from farm characteristics like location, farm size, fertilizer application level, animal density, grass production and sugar beet or potato area. The results of the model are satisfying for nutrient surplus (R2=0.9) and nitrogen NUE (R2=0.4). Estimation of phosphorus NUE was not satisfactory. High surpluses are associated with high fertilizer applications, high animal density and high grass production while an increasing share of sugar beets leads to lower surpluses. A high nitrogen NUE is associated with low fertilizer applications, low animal density and little grass production, and with a high sugar beet area share. Results suggest that, with exception of sugar beet, fertilizer recovery in Poland is very low. Sugar beet, however, combines high fertilizer applications with low surpluses and high NUE. The outcome of the model can be used in the design of environmental policies. The paper ends with some remarks on the type of measures that can be taken, and the effects these will have on private farms in Poland.
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  • 26
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    Nutrient cycling in agroecosystems 46 (1996), S. 81-90 
    ISSN: 1573-0867
    Keywords: elemental sulfur ; granule size ; nitrogen ; phosphorus ; potassium ; S oxidation ; sulfur fertilizers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Experiments were undertaken to determine the effect of granule size and nutrients in granulated compound fertilizers fortified with finely divided elemental sulfur (So) on the rate of So oxidation. In one experiment, So was banded together with or apart from triple superphosphate (TSP) while in two others, So was granulated with nutrient and inert carriers. A fourth experiment examined response to S in an So-fortified TSP from a range of granule sizes. Response and, in some cases, So recovery (using 35S labels) by test crops (maize, wheat, upland rice) was measured. In all experiments, P mixed with So increased plant growth and S recovery above treatments in which P and So were physically separated. There was however, no effect of distance of separation on S recovery. In one experiment, N as urea and N and P as diammonium phosphate (DAP) were also found to enhance response to So although to a lesser degree than P alone. These observations were attributed to a nutritional requirement of So-oxidizing microorganisms for P and N. Granulation of So with carriers also influenced oxidation rate, as inferred from the fertilizer S recovery. For a given So concentration, the effect was inversely proportional to the mean diameter of granules. It is shown that this relationship can be explained if one assumes that So particles in granules collapse into a fixed number of aggregates per granule irrespective of granule size when the soluble nutrient carrier dissolves and diffuses away from the point of application.
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    Nutrient cycling in agroecosystems 46 (1996), S. 53-70 
    ISSN: 1573-0867
    Keywords: crop ; emission ; fertilizer ; nitrogen ; nitrous oxide ; soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This analysis is based on published measurements of nitrous oxide (N2O) emission from fertilized and unfertilized fields. Data was selected in order to evaluate the importance of factors that regulate N2O production, including soil conditions, type of crop, nitrogen (N) fertilizer type and soil and crop management. Reported N2O losses from anhydrous ammonia and organic N fertilizers or combinations of organic and synthetic N fertilizers are higher than those for other types of N fertilizer. However, the range of management and environmental conditions represented by the data set is inadequate for use in estimating emission factors for each fertilizer type. The data are appropriate for estimating the order of magnitude of emissions. The longer the period over which measurements are made, the higher the fertilizer-induced emission. Therefore, a simple equation to relate the total annual direct N2O−N emission (E) from fertilized fields to the N fertilizer applied (F), was based on the measurements covering periods of one year: E=1+1.25×F, with E and F in kg N ha-1 yr-1. This relationship is independent of the type of fertilizer. Although the above regression equation includes considerable uncertainty, it may be appropriate for global estimates.
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  • 28
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    Nutrient cycling in agroecosystems 46 (1996), S. 179-187 
    ISSN: 1573-0867
    Keywords: electrical conductivity ; leaching ; nitrogen ; pH ; phosphorus ; potassium ; release pattern ; slow-release fertilizers ; temperature
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We studied the effect of temperature on the release of N, P, and K from slow-release fertilizers (SRF). The study was conducted in micro-lysimeters filled with moist peat medium. Increasing the temperature from 4 to 12°C slightly increased N release from three different slow-release N (SRN) carriers with different particle sizes and coating thicknesses. At 21°C the rate of release was significantly different than the other two temperatures. Urea formaldehyde (UF), sulphur coated urea (SCU) and coated calcium nitrate (CCN), incubated in sphagnum moss peat, released between 3 and 20% of the applied N in six weeks. For eight synthetic and organic NPK carriers, the release pattern was similar to UF and SCU. However, the leaching losses of N from the NPK fertilizers were up to twenty times more than for the SRN products. Except for Osmocote® and Duna, which released 30–40% of the applied N as mineral-N within six weeks, all other slow-release and slowly mineralized NPK carriers acted like readily water-soluble compound NPK. Temperature did not affect the nutrient release from NPK fertilizers.
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  • 29
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    Nutrient cycling in agroecosystems 50 (1998), S. 271-276 
    ISSN: 1573-0867
    Keywords: nitrogen ; monitoring ; dairy farming ; simulation models ; risk assessment ; soil variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract At the ‘De Marke’ experimental farm a dairy farm was set up with the aim of meeting environmental and economic goals. The farm management with respect to nitrogen emphasized reduction of fertilization and a cattle grazing system that should result in nitrate concentrations in the groundwater below the EC-directive level of 11.3 mg l-1 nitrate-N. At six sites in six different fields of ‘De Marke’, these concentrations were monitored for 4 years. A direct comparison with the chosen limit was possible for these sites, but an evaluation of the environmental achievements of the farming system at farm level was also required. This was achieved by using simulation models and additional information about soils and field management. Based on multiple soil profile descriptions, frequency distributions of model output were generated, allowing a risk assessment for the total farm. The probability of exceeding the chosen threshold value of 11.3 mg l-1 nitrate-N during the period of summer 1991- spring 1995 was 63% for the whole farm, with marked differences between years, crops and hydrological conditions.
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    Nutrient cycling in agroecosystems 50 (1998), S. 321-324 
    ISSN: 1573-0867
    Keywords: nutrient modelling ; leaching ; nitrogen ; phosphorus ; schematization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In context of preparing the Fourth National Policy Document on Water Management in the Netherlands effects of different scenarios of fertilizer management on nitrogen (N) and phosphorus (P) leaching from rural areas into Dutch surface waters were analyzed. The manuscript offers insight into the model instrument that is used to simulate the different scenarios. Main parts of the modelinstrument are: a procedure to schematize the Netherlands in horizontal areal units, field scale mechanistic models for water and nutrient behaviour in the soil and an empirical model for fertilizer additions.
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    Nutrient cycling in agroecosystems 52 (1998), S. 277-287 
    ISSN: 1573-0867
    Keywords: dairy farming ; efficiency ; nitrogen ; organic ; pig farming ; surplus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study is in two parts. In the first part, nitrogen (N) losses per unit of milk and meat in Danish conventional and organic pig and dairy farming were compared on the basis of farm data. In the second part, organic and conventional dairy farming were compared in detail, using modelling. N-surpluses at different livestock densities, fodder intensities, and soil types were simulated. Finally, simulated N-surpluses were used in national scenarios for conversion to organic dairy farming in Denmark. In Part one, pig farming was found to have a higher N-efficiency than dairy farming. Organic pig production had a lower N-efficiency and a higher N-surplus per kg meat than conventional pig production. The possibilities to reduce N-loss by conversion to organic pig production therefore appear to be poor. Organic dairy farming had a higher N-efficiency and a lower N-surplus per kg milk than conventional dairy farming. Conversion from conventional to organic dairy farming may therefore reduce N-losses. In Part two, a positive correlation between livestock density and N-surplus ha-1 was found for dairy farming. For all simulated livestock densities, fodder feeding intensities and soil types, organic systems showed a lower N-surplus per unit of milk produced than conventional systems. National scenarios for dairy farming showed that the present Danish milk production could be achieved with a 24% lower total N-surplus if converted from intensive conventional farming to extensive organic farming. At the same time, N-surplus ha-1 and N-surplus (t milk)-1 would be lowered by 50% and 25% respectively. Changing from intensive to extensive conventional dairy farming with a livestock density equal to that in the organic scenario resulted in a reduction in N-surplus ha-1 of 15%. It was concluded that a reduction in total N-loss from agriculture is possible by converting from conventional to organic dairy farming but at the cost of either lower production on the present dairy farm area, or the current production on a substantially larger area.
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  • 32
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    Nutrient cycling in agroecosystems 52 (1998), S. 289-301 
    ISSN: 1573-0867
    Keywords: food production and consumption ; mitigation strategies ; nitrogen ; nitrogen emission ; nitrous oxide ; sustainability of human N cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract During the past four decades the authors perceive that an increasing non-sustainability (Disintegration) within the agriculture , human nutrition, waste management complex has occurred both in Germany and the European Union. Compared to the basic needs of the population for nutritive energy, fat and protein, we estimate that the production and consumption of food and feed is more than 50% higher than necessary. Using nitrogen (N) input into German agriculture in 1991/92 as an example, we estimated that the N input of 191 kg ha-1 was 2 to 3 times too high. This high N input resulted in the net biomass production of 45 kg ha-1, a 25% efficiency. This inefficiency causes emissions of reactive N and other nutrient compounds into the hydrosphere and atmosphere that were 2 to 8 times too high. For example the contributions of agriculture to the total annual N2O–N emissions of Germany (during 1990–1992), Europe (1990) and of the world (1989) were 110, 691 Gg and 6.7 Tg or 52, 62 and 41%, respectively. The authors demonstrate that emissions of N and P from Germany and EU waste water management systems are also higher than necessary because nutrient recycling is not practiced extensively. Excessive food production and consumption has made the agriculture/human nutrition/waste and waste water complex, like the energy/transportation complex, a main cause of new transboundary environmental damage such as soil and water acidification, hypertrophication of near-natural terrestrial and aquatic ecosystems and climate change. We propose that a sustainable food production/consumption system can be developed that is based both on need-oriented production and consumption with no net exports and on recovery, recycling and more efficient use of nutrients. Using N as an example, the authors show which short and long term action aims must be set and realized by the year 2015, to meet environmental, economical and social sustainability requisites. The suggested, assumed sustainable N balance for German agriculture is characterized by a critical annual input and surplus maximum of 80 and 45 kg N ha-1 respectively, which should almost double biomass production efficiency for N utilization. This estimate is based on reducing animal stocking rates to 0.5 gross weight unit ha-1 to attain no net mineralization or immobilization of N in the soil.
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    Nutrient cycling in agroecosystems 52 (1998), S. 171-178 
    ISSN: 1573-0867
    Keywords: atmospheric deposition ; boreal forests ; critical loads ; forest growth ; gas losses ; leaching ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen is brought into natural ecosystems from the atmosphere through N-fixation and deposition of NH 4 + and NO 3 - as wet and dry deposition. N is lost from terrestrial ecosystems unaffected by human activities by leaching or as gas, but the losses from different forest-and vegetation types are poorly quantified. The leaching is hampered by uptake in the vegetation and by immobilisation by soil organisms. The gas loss of N in the form of N2O and N2 appears to be rather small, but the loss of NO is unknown. Human activities such as those leading to the increased atmospheric dry and wet deposition of N, may affect the N-losses, but the increase in losses are likely to be very dependent on the amounts of N deposited. The present paper discusses the fate of the N deposited under the existing pollution climate in Norway where N deposition above the natural background has taken place for at least 50 years. The deposition today varies from about 15–20 kg N ha-1 yr-1 in the southernmost parts of the country to background values of about 2 kg ha-1 yr-1 in the north. Even in areas with the highest loads there are no clear indications that N-leaching in forest ecosystems has increased to ‘unacceptable’ levels. The main reason for this appears to be that most of the N deposited are immobilised in forest soils and utilised by forest trees and ground cover vegetation to increased biomass production. At present therefore, no clear signs of N-saturation can be found. This is in relatively good accordance with estimates of critical loads for N in Norwegian coniferous forests. Estimates of immobilisation, gas losses, net uptake in vegetation, biological fixation as well as a figure for ‘acceptable’ leaching, indicate that the long-term critical load of N might be of the order 10 to 30 kg N ha-1 yr-1. Most of the N deposited from the atmosphere appears therefore so far to be retained in the forests and only a small proportion seems to be lost to ground-and surface waters.
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    Nutrient cycling in agroecosystems 53 (1999), S. 209-218 
    ISSN: 1573-0867
    Keywords: cattle slurry ; fertilizer splitting ; nitrogen ; recovery ; residual nitrogen ; Zea mays L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The recovery of soil mineral nitrogen (N) by crops, and its subsequent utilisation for dry matter (DM) production may be increased when the application of N is postponed until after crop emergence. The significance of this strategy for silage maize was studied in nine field experiments on Dutch sandy soils from 1983 to 1988. In five experiments the effect of slurry applied before planting at a rate of circa 66 m3 ha-1, was compared to the effect of a similar rate of which half was applied before planting and half at the 4–6 leaf stage. In the 4-6 leaf stage slurry was either injected or banded. In four other experiments the effect of mineral fertilizer-N splitting was studied. In these experiments, 30 m3 ha-1 cattle slurry, applied before planting, was supplemented with mineral fertilizer-N at rates ranging from 40 to 160 kg ha-1, either fully applied before crop emergence or split. When split, 40 kg ha-1 of the mineral fertilizer-N rate was banded at the 4–6 leaf stage. According to balance sheet calculations, substantial losses of slurry N and mineral fertilizer-N occurred during the growing season. Losses were compensated for, however, by apparent mineralization, ranging from 0.34 to 0.77 kg N ha-1 day-1. Split applications of cattle slurry had a significant positive effect on the DM yield in two out of five experiments compared to the conventional non-split application, but only when the post-emergence slurry application was banded which is no longer in accordance with present legislation. Split applications of mineral fertilizer-N had a significant positive effect in one experiment where rainfall was excessive but not in the others. The results provide insufficient evidence to recommend farmers to split applications. Soil mineral N sampling at the 4–6 leaf stage should hence be considered a control on the appropriateness of early N applications after exceptional weather conditions rather than a routine observation on which the post-emergence N dressing is to be based in a deliberate splitting strategy. Our data suggest that the financial return of a 40 kg ha-1 supplementation with mineral fertilizer-N, was questionable when more than 175 kg N ha-1 were found in the upper 0.6 m soil layer at the 4–6 leaf stage.
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    Nutrient cycling in agroecosystems 53 (1999), S. 259-267 
    ISSN: 1573-0867
    Keywords: farming systems ; nitrogen ; nitrogen budgets ; sustainability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three approaches to nitrogen budgeting were developed and their ability to quantitatively describe nitrogen cycling in a fertilizer based and a grass–clover based beef system tested. Budgets ranged in complexity from the Economic Input:Output (EIO) budget, which accounted simply for purchases and sales of nitrogen over the farmgate, through the Biological Input:Output (BIO) budget, which included estimates of biological nitrogen fixation and attempted to partition losses into leaching and gaseous forms, to the Transfer:Recycle:Input:Output (TRIO) budget, which also accounted for key soil processes. Nitrogen unaccounted for in the fertilized system decreased with increasing budget complexity (285, 212 and 188 kg ha-1 yr-1 unaccounted for by the EIO, BIO and TRIO budgets, respectively). In the legume based grass–clover system, the EIO budget did not accurately describe total nitrogen inputs as it did not include 146 kg ha-1 yr-1 from symbiotic nitrogen fixation. In the grass–clover system, nitrogen unaccounted for was again greater using the BIO than the TRIO budget (103 and 79 kg ha-1 yr-1, respectively). In conclusion, the most complex budgeting approach (TRIO) was able to account for the fate of a greater proportion of nitrogen inputs than the simpler approaches. However, the perceived success of the different approaches was strongly dependent on the precise objective.
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    Nutrient cycling in agroecosystems 55 (1999), S. 1-6 
    ISSN: 1573-0867
    Keywords: chlorophyll meter ; irrigation ; LAI ; nitrogen ; Spring Wheat
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A Spring Wheat crop was grown using three irrigation levels and nitrogen rate applications to evaluate chlorophyll meter measurements as a possible nitrogen nutrition index for modelling. These measurements yielded the most reliable indications at Zadoks GS45. The lower limit indicating severe nitrogen deficiency in the leaves was approximately 35 SPAD units while the upper limit of 45 SPAD units, indicated an excess consumption.
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    Nutrient cycling in agroecosystems 55 (1999), S. 89-94 
    ISSN: 1573-0867
    Keywords: algae ; flooded soils ; N cycling ; nitrogen ; 15N ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Algal N labelled with 15N added to a flooded soil in laboratory columns without plants was studied to determine the changes over time in the fate of N assimilated by algae and to study how its fate is affected by (a) exclusion of light simulating complete closure of the rice canopy, and (b) addition of fertilizer-NH4 *. In the light but with no added fertilizer-N there was little net mineralization of the added algal N during the first 4 weeks, but after 8 weeks 42% had been mineralized, of which 95% was denitrified. Exclusion of light caused net mineralization to proceed more rapidly in the first 4 weeks due to the death of algal cells and lowered reassimilation. After 8 weeks 51% had been mineralized, of which 54% was denitrified, 16% volatilized and 30% was present as KCl exchangeable NH4 +-N. Application of fertilizer-NH4 + apparently caused mineralization of 25% of the algal N within one week but the results were probably affected by pool substitution in which labelled N mineralized to NH4 +-N was diluted with fertilizer – NH+ 4 and then immobilized leaving more labelled NH4–N in the mineral pool. After 8 weeks, 42% of algal N had been mineralized, of which 69% was estimated to have been denitrified, 19% lost through NH3 volatilization and 12% remained as extracted NH4 ++NO- 3. Uptake of N by a rice crop would reduce the gaseous losses. Algal N was mineralized quickly enough to be available during the growing season of a rice crop and, depending on field conditions, algae may have a role in assimilating N and protecting it from loss as well as being a major driving force for NH3 volatilization through diurnal increases in pH.
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    Nutrient cycling in agroecosystems 1 (1980), S. 137-155 
    ISSN: 1573-0867
    Keywords: energy consumption ; energy production ; photosynthesis ; mineral fertilization ; biomass and live energy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract 1. The world consumption of energy is roughly 250 EJ. It increases with the level of technology and gross national product of a country. More than 83% of world energy consumption is used by the industrialized countries with one third of the world population; not quite 17% is used by the developing countries with two thirds of the world population. The world's resources of fossil fuels are estimated at 364,560 EJ; about 5–13% of this, and 31% in the case of natural gas, are considered reserves that are economically recoverable and utilizable with current technologies. 2. Agriculture's share of the economy's energy consumption in the Federal Republic of Germany is about 3.4%. It was five times higher per hectare of agricultural land in 1975 than in 1880, but the productivity of the energy was only half as high because of the enormous increase in productivity per unit of labor and area. In absolute terms, however, energy production per unit area increased tremendously, with gross agricultural production two and a half times its earlier size. 3. As a producer of plant material, agriculture qualifies as an energy producer, while as a producer of livestock it also is an energy consumer. In fact, through plant production agriculture becomes the only branch of our economic system that produces more energy than it consumes as fossil energy. Agriculture uses about 40% of its energy requirement for fuel, about 20% for machinery repair and replacement, 30% for mineral fertilizers, about 10% for electricity, and 1–2% for chemical crop protection. Forestry can be evaluated as particularly favorable from the energy viewpoint, while hothouse crops are very unfavorable. Agricultural chemicals support the energy output of green plants; agriculture as a whole is on balance energetically. 4. Solar energy and photosynthesis are the primary sources of energy to our plant. About 3 million EJ solar energy are radiated to the earth annually; 3000 EJ are fixed photosynthetically (2 ⋅ 1011 tons vegetable matter); the food requirement of 4 billion people is 15EJ. Another item of interest on the periphery of the energy balance is the enrichment of our atmosphere with oxygen, which has been accomplished for millions of years solely by the photosynthesis of green plants. 5. Through their additional yield effect, mineral fertilizers increase the energy output of plants more strongly than just the equivalent of the energy input. They cause the plant to produce more foliage and thereby promote more intensive assimilation, which means that mineral fertilizers enable the plant to utilize free solar energy better. A calculation of the energy involved in long-term field trials in cereals disclosed energy input: energy output ratios of 1:5.8 and 1:6.1. 6. Chemical crop protection has a similar effect since it protects against loss of plantproduced energy. Based on an average energy expenditure of 263 MJ ha−1 per kg ha−1 ‘typical’ active ingredient for a crop protection product, additional yields of only 4–4.5% — or considerably less in the case of high-energy crops such as cereals or sugar beets — would be sufficient to cover the energy expenditure; as a rule, however, the productivity of the chemical crop protectants is higher. The biological potential of our crops to utilize solar energy also has been improved considerably compared to earlier times — with cereals, for example, from 0.25% per unit area during the Middle Ages to 1.5% today; theoretically 4% is possible. The thesis that agrochemical aids in agriculture and horticulture are a waste of energy is unjustified. 7. Biomass also creates energy. Experts estimate the utilizable annual production of biomass in the Federal Republic of Germany to be 30 million tons mineral coal units (1 coal unit = 29.3 MJ), whereby undersized and refuse wood, straw and biogas are of special significance. Especially “fuel forests' of, for example, willows, poplars and alders could produce the equivalent of 486,000 MJ by way of 30 tha−1 biomass, contributing sizably to the fuel supply of the nation; at the moment, the conventional form of forestry produces only 30,240 MJ. It is considered feasible in Sweden to supply the entire energy requirement of the country from 93,000 km2 of ‘fuel forest’, and it must be remembered that mineral fertilization could be used to increase the productivity of land used for this purpose relatively quickly if the need were to become acute. The extraction of alcohol from crops offers other interesting aspects; the currently highest yield fuel crops (sugar beet, sugarcane and cassava) produce between 4,900 and 10,700 l ha−1 alcohol. 8. The energy problem of modern economies will not find its complete answer in the green plant. Prudent and well contemplated use of the green plant, however, may eventually do much to take the edge off today's energy dilemma.
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  • 39
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    Nutrient cycling in agroecosystems 3 (1982), S. 13-16 
    ISSN: 1573-0867
    Keywords: cocoa ; nitrogen ; phosphorus ; soil analysis ; Nigeria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract No extensive investigation on the effect of fertilizers on Amazon cocoa variety (Theobroma cacao L.) has been performed in Nigeria. Therefore eight fertilizer treatments involving nitrogen and phosphorus, replicated six times at four locations across southern Nigeria, were established in 1973. The four N levels (N0, N1, N2, N3) involved were 0, 80, 160 and 240 kg ha−1 y−1, and the two P levels (P0, P1) were 0 and 67 kg ha−1 y−1. Results of the first 5 years of fertilizer application are reported. Response to P was observed at all locations, and the response was statistically significant at 2 of the locations. There was no response to the application of nitrogen. The data suggest, however, that there is only a response to phosphorus when nitrogen is applied.
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  • 40
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    Nutrient cycling in agroecosystems 3 (1982), S. 17-23 
    ISSN: 1573-0867
    Keywords: nitrogen ; potassium ; fertilization ; Norway spruce ; quartzite ; podzol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In young spruce plantations on mineral soils the growth of the trees is improved by N application only in rare cases, and by P or K application only on sites over parent rock, which is poor in available fractions of these nutrients. Interpretation of a field trial showed that on extremely poor quartzitic soils potassium deficiency limited growth during the first years of crop development. Potassium deficiency ended at pole stage (about 15 years after planting). N deficiency began at sapling stage (about 8 years after planting) and increased later. On quartzitic and similar sites with a low cation-exchange capacity, appropriately phased applications of K and N markedly improve the site index and yield level of young spruce stands. Thus, it was shown that the stock of stemwood can be raised from 3 to 13 m3 per ha up to an age of 16 years.
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  • 41
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    Nutrient cycling in agroecosystems 3 (1982), S. 37-62 
    ISSN: 1573-0867
    Keywords: ammonium-N ; distillation ; fertilizers ; mass spectrometer ; nitrate-N ; nitrogen ; urea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Although the stable isotope15N is an indispensable tool in research to trace the fate of fertilizer nitrogen in soil/plant systems, the analytical methods used in this research are time consuming and prone to many errors. This paper outlines the methods used in an international program of nitrogen research coordinated by the International Fertilizer Development Center (IFDC). The different steps in the digestion, distillation, and isotope ratio analysis of15 N-labeled soils, plant material, and fertilizers are described. Details on the use of a series of controls to check the precision and accuracy of the methods are also given. It is hoped that this comprehensive description of procedures will encourage the expanded and proper use of15N.
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  • 42
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    Nutrient cycling in agroecosystems 3 (1982), S. 379-383 
    ISSN: 1573-0867
    Keywords: band placement ; computer modelling ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A model was developed to describe the distribution of mineral nitrogen when applied in a fertilizer band, taking into account nitrification and diffusion. Good agreement was found between the calculated values and those measured in a field experiment.
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  • 43
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    Nutrient cycling in agroecosystems 42 (1995), S. 185-192 
    ISSN: 1573-0867
    Keywords: 15N ; nitrogen ; soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The use of15N as a tracer in soil/plant research is examined. The limitations of the so-called Ndff approach are discussed to show the need to consider not just the fate of the added label but also the path that was followed and the rate of the transformation. The development of15N isotope dilution techniques to determine gross rates of nitrogen transformation in soil is reviewed with some indications as to the further development of the approach.
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  • 44
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    Nutrient cycling in agroecosystems 47 (1996), S. 243-250 
    ISSN: 1573-0867
    Keywords: fertilizer value ; nitrogen ; phosphorus ; poultry manure ; urea ; wetland rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Poultry manure applied alone or in combination with urea at different N levels was evaluated as a N source for wetland rice grown in a Fatehpur loamy sand soil. Residual effects were studied on wheat which followed rice every year during the three cropping cycles. In the first year, poultry manure did not perform better than urea but by the third year, when applied in quantities sufficient to supply 120 and 180 kg N ha−1, it produced significantly more rice grain yield than the same rates of N as urea. Poultry manure sustained the grain yield of rice during the three years while the yield decreased with urea. Apparent N recovery by rice decreased from 45 to 28% during 1987 to 1989 in the case of urea, but it remained almost the same (35, 33 and 37%) for poultry manure. Thus, urea N values of poultry manure calculated from yield or N uptake data following two different approaches averaged 80, 112 and 127% in 1987, 1988 and 1989, respectively. Poultry manure and urea applied in 1:1 ratio on N basis produced yields in between the yields from the two sources applied alone. After three cycles of rice-wheat rotation, the organic matter in the soil increased with the amount of manure applied to a plot. Olsen available P increased in soils amended with poultry manure. A residual effect of poultry manure applied to rice to supply 120 or 180 kg N ha−1 was observed in the wheat which followed rice and it was equivalent to 40 kg N ha−1 plus some P applied directly to wheat.
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  • 45
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    Nutrient cycling in agroecosystems 48 (1997), S. 241-246 
    ISSN: 1573-0867
    Keywords: barley ; denitrification ; fertilizerefficiency ; mineralization ; nitrogen ; sludge
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Enhanced microbial activity following sewage sludge land application may affect soil N cycling and, therefore, plant available N. We studied the effect of anaerobically treated sewage sludge on N-fertilizer efficiency and on some aspects of the soil N cycling. Field plots (3 m × 9 m) sown with barley (Hordeum vulgare L.) in November were amended with a) sludge (80 mg ha-1) and ammonium nitrate (150 kg N ha-1), b) ammonium nitrate (150 kg ha-1) only, c) or left unamended. Monthly soil samples were taken from 0 to 20-and 20 to 50-cm depths to determine soil inorganic N (NH4 +, NO3-). Denitrification in the upper 20-cm horizon was estimated by measuring N2O+N2 emission from undisturbed soil samples by the acetylene-inhibition technique. Crop yield parameters were analysed before harvesting, and grain production was recorded. With respect to the control, the yield increase for the N-fertilizer treatment was 85% and 45% for the sludge + N-fertilizer treatment. The decrease of N-fertilizer efficiency in sludge amended plots was presumably due to a decrease in spring plant available N. Presumably, microbial immobilisation and denitrification in organic amended treatments were responsible for the decrease in N-fertilizer efficiency.
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  • 46
    ISSN: 1573-0867
    Keywords: Phosphorus ; nitrogen ; iron ; VAM ; Rhizobium ; faba-bean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of rhizobial inoculation, vesicular arbuscular mycorrhizal (VAM) fungi on the growth, P, N and Fe uptake by faba-bean plants (Vicia faba L.V. Giza 2) grown in virgin sandy soil, treated with super or rock-P were studied under green-house conditions. The earthern pots received a half of the recommended rate of P either as single super phosphate or rock-P in the rate of 20 mg P/kg soil, and calcium ammonium nitrate was added in the rate of 10 mg N/kg soil. Iron was applied in two levels 0 and 5 mg Fe/kg soil, in the form of iron sulphate (FeSO4·7H2O). Dry matter yield, as well as P, N and Fe-uptake were determined. Nodule numbers and their dry weights, spore numbers and mycorrhizal root infection were determined. Results indicated that, fungal infection and rhizobial inoculation either alone or in combination increased dry matter yield as compared to uninoculated plants, whereas the percentages increase in dry matter were 34, 26 and 57% in case of super-P application, while they were 56, 47 and 89% in case of rock-P when inoculated withRhizobium, VAM and dual inoculation respectively. Also P, N and Fe uptake were significantly increased due to inoculation, and dual inoculation resulted the highest effect. Generally, inoculation withRhizobium and/or mycorrhizae can remove the deficient effect of P and Fe on N2-fixation and plant growth in the soil of low nutrients content.
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  • 47
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    Nutrient cycling in agroecosystems 48 (1997), S. 37-50 
    ISSN: 1573-0867
    Keywords: greenhouse gases ; nitrogen ; NOx ; nitrous oxide ; ozone precursors ; trace gases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Over 60 published papers reporting field measurements of emissions of nitric oxide (NO) from soil are reviewed, and over 100 annual estimates of NO emissions were made for various types of ecosystems, including agricultural fields. These data were stratified by biome and the mean of each stratum was multiplied by an estimate of the biome area. A few strata were identified as clearly having low NO emissions: montane forests, swamps and marshes, tundra, and temperate forests that are not heavily affected by N deposition. The largest emissions were observed in tropical savanna/woodland, chaparral, and cultivated agriculture, but variation in NO emissions within these strata was also large. Although the stratification scheme fails to partition this within-stratum variation, it does clearly identify these biomes as globally important sources of NO and as areas where more research is needed to investigate within-biome variation in NO emissions. It is too early to tell whether differences in NO emissions between temperate and tropical agriculture are significant, but it is clear that agriculture is an important source of NO and that management practices affect NO emissions. The best current estimate of the global soil source of NO is 21 Tg N yr-1. Adsorption of NOx onto plant canopy surfaces may reduce emissions to the atmosphere to as low as 13 Tg N yr-1, although the absorption effect is probably smaller than this. An error term for the global estimate is difficult to determine, but it is at least ±4 and perhaps as large at ±10 Tg N yr-1. Hence, only modest progress has been made in narrowing uncertainties in the estimate of the global soil source of NO, although some published lower estimates appear unlikely. This inventory reconfirms that the soil source of NO is similar in magnitude to fossil fuel emissions of NOx. Further narrowing of the uncertainty of the estimate of global soil NO emissions will require more sophisticated and carefully chosen stratification schemes to address variation within biomes based on soil fertility, soil texture, climate, and management and will require linking this type of inventory and stratification with mechanistic models.
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  • 48
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    Nutrient cycling in agroecosystems 5 (1984), S. 371-382 
    ISSN: 1573-0867
    Keywords: nitrogen ; phosphorus ; dry areas ; apparent recovery fraction ; fertilizer management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Agronomic efficiency (AE) is defined as the increase in economic yield of a crop per unit fertilizer applied. Components of AE are physiological efficiency and apparent recovery fraction. The latter can be further separated into uptake efficiency and availability index. Ways to increase the nitrogen fertilizer use efficiency in rainfed agriculture through fertilizer management are discussed.
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  • 49
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    Agroforestry systems 42 (1998), S. 181-194 
    ISSN: 1572-9680
    Keywords: Acacia ferruginea ; Albizia lebbeck ; Faidherbia albida ; nitrogen ; sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of three nitrogen fixing tree (NFT) species viz., Faidherbia albida, Acacia ferruginea, Albizia lebbeck and four nitrogen levels (0, 20, 40 and 60 kg N ha−1) on intercropped rainfed sorghum was investigated at Hyderabad, India. The trees were established in shallow alfisols during 1985 with a spacing of 4 × 4 m. Intercropping was done in 1993 and 1994. The effect of trees on radiation interception, soil moisture, crop growth, yield components and yield of sorghum was examined. Association of tree species reduced grain and dryfodder yields of sorghum to an extent of 12 to 40% compared to sole crop situation. The reduction was maximum under A. lebbeck, minimum with F. albida and moderate under A. ferruginea. Application of 40 kg N ha−1 resulted in increased grain and dryfodder yields over other levels. Crop growth in terms of LAI and drymatter had similar response. The receipt of photosynthetically active radiation (PAR) was low under NFTs compared to open situation. The relative PAR intercepted under the trees was in the order: F. albida 〉 A. ferruginea 〉 A. lebbeck. Soil moisture status was more favourable under F. albida than under the other tree species. Soil moisture at all stages of crop growth was more in sole crop situation.
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  • 50
    ISSN: 1572-9680
    Keywords: fibre ; intake ; nitrogen ; palatability ; tannin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aim of this work was to assess whether degradability, gas production or chemical constituents could predict the preference of browses. Forty tropical browse species leaves with a crude protein (CP) content ranging from 79 to 307 g kg-1 DM were used for this study. The neutral detergent fibre (NDF) ranged from 220 to 694 g kg-1 DM, while acid detergent fibre (ADF) ranged from 146 to 523 g kg-1 DM. The NDF-bound nitrogen (NDFN) and ADF-bound nitrogen were particularly high in Calliandra calothyrsus, Acacia polyacantha, Sesbania sesban, Acacia venosa and Acacia hockii. High levels of tannins were observed in Acacia species especially A. dolichocephala, A. hockii, A. microbotrya and A. salicina. High levels were also observed in Flemingia macrophyla and Leucaena pallida. The browse species differed (P 〈 0.05) in DM in sacco degradability coefficients. High potential degradability (PD) and effective degradability (ED) were observed in Sesbania spp, Moringa stenopetala, Indigofera arrecta, Chamaecytisus palmensis and Atriplex spp. The browses differed (P 〈 0.05) in asymptotic gas (Ag) production (ml g-1 OM), but had similar (P 〉 0.05) times of incubation at which half of the asymptotic gas had been formed. Preference and DM intake were positively correlated (P 〈 0.01) to NDFN, but negatively correlated (P 〈 0.05) to NDF and ADF. The PD and Ag were negatively (P 〈 0.001) related to NDF, ADF and lignin. Total phenols (TP) and condensed tannins (CT) were negatively (P 〈 0.05) related to PD, ED and Ag. A positive correlation was observed between CT and NDF-bound condensed tannins (r = 0.55, P 〈 0.001) and, CT and TP (r = 0.40, P 〈 0.01). Prediction equations were poor for DM intake and preference, moderate for gas production and good for potential and effective degradabilities. The phenolic components were more related to dry matter degradation and gas production than to preference and dry matter intake. NDFN and Ag made a positive contribution to both preference and DM intake. It was concluded that chemical constitutes such as N, NDF, NDFN, ADF and lignin are essential to predict the nutritive value of browses.
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  • 51
    ISSN: 1572-9702
    Keywords: tomato plants ; susceptibility ; mite ; tridecan-2-one ; nitrogen ; potasium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this work was to study the effect of NK fertilization levels and canopy height on the resistance of Lycopersicon hirsutum and Lycopersicon esculentum to Aculops lycopersici (Acari: Eriophydae). The effects of NK fertilization levels and canopy height in the leaf size and density of trichomes and their effects on tridecan-2-one (2-TD) and undecan-2-one (2-UD) limiting the attack of A. lycopersici on tomato plants were assessed. Different NK fertilization levels had no effect on the resistance of L. hirsutum to A. lycopersici. No significant differences were found in attack rates of this mite on leaves of the top and median parts of L. hirsutum canopy. The type and density of trichomes were the main determining factor of A. lycopersici attack on tomato plants. High trichome densities and type VI glandular trichomes which produce tridecan-2-one are important resistance factors on tomato plants. L. hirsutum showed a high resistance level to A. lycopersici due to high densities of type VI glandular trichomes and consequently higher levels of tridecan-2-one in its leaves.
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    Landscape ecology 10 (1995), S. 337-348 
    ISSN: 1572-9761
    Keywords: fire ; nitrogen ; NDVI ; transect ; watershed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transects across watersheds with varying fire histories and remotely-sensed data were used to study vegetation-resource interactions in a tallgrass prairie in Kansas. Paired plots (fertilized, control) were established along these transects and sampled for grass and forb biomass during the 1989 and 1990 growing seasons. Fertilization resulted in significant production responses in grass and total biomass on the west slopes of the annually burned (ID) and infrequently burned (N4) watersheds for both years (p = 0.05). In 1989, fertilization also produced a significant increase in grass biomass on the west slope of the unburned transect (p = 0.05), however, total production was not significantly increased. East slopes were insensitive to nitrogen additions. Differences in production response along these transects were assessed by testing the interaction between fertilization response and slope position. Significant interactions occurred on both 1D and N4, but only in 1990. Production data for both years were also compared to Normalized Difference Vegetation Index (NDVI) values derived from thematic mapper (TM) images for 1989 and 1990. When differences among transects or watersheds were statistically different, a positive relationship between NDVI and biomass was observed. NDVI values accurately reflected the spatial patterns of production along these transects for both years although not necessarily the magnitude.
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  • 53
    ISSN: 1572-9761
    Keywords: forest productivity ; NPP ; runoff ; climate ; nitrogen ; northeastern U.S. ; modeling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We used the PnET-II model of forest carbon and water balances to estimate regional forest productivity and runoff for the northeastern United States. The model was run at 30 arc sec resolution (approximately 1 km) in conjunction with a Geographic Information System that contained monthly climate data and a satellite-derived land cover map. Predicted net primary production (NPP) ranged from 700 to 1450 g m2 yr1 with a regional mean of 1084 g m2 yr1. Validation at a number of locations within the region showed close agreement between predicted and observed values. Disagreement at two sites was proportional to differences between measured foliar N concentrations and values used in the model. Predicted runoff ranged from 24 to 150 cm yr1with a regional mean of 63 cm yr1. Predictions agreed well with observed values from U.S. Geologic Survey watersheds across the region although there was a slight bias towards overprediction at high elevations and underprediction at lower elevations. Spatial patterns in NPP followed patterns of precipitation and growing degree days, depending on the degree of predicted water versus energy limitation within each forest type. Randomized sensitivity analyses indicated that NPP within hardwood and pine forests was limited by variables controlling water availability (precipitation and soil water holding capacity) to a greater extent than foliar nitrogen, suggesting greater limitations by water than nitrogen for these forest types. In contrast, spruce-fir NPP was not sensitive to water availability and was highly sensitivity to foliar N, indicating greater limitation by available nitrogen. Although more work is needed to fully understand the relative importance of water versus nitrogen limitation in northeastern forests, these results suggests that spatial patterns of NPP for hardwoods and pines can be largely captured using currently available data sets, while substantial uncertainties exist for spruce-fir.
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    Landscape ecology 11 (1996), S. 27-38 
    ISSN: 1572-9761
    Keywords: abandoned channels ; floodplain ; nitrogen ; organic matter ; phosphorous ; potassium ; sediment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nature of sediments in abandoned channels is an important component of their development as floodplain wetlands. The texture, organic matter, phosphorous, potassium, and nitrogen content of sediments were determined for abandoned channels along the Iowa and Cedar Rivers near their confluence in Iowa. Differences in the levels of these constituents were examined among categories of three landscape gradients: present connectivity to the river, time since abandonment, and proximity to agricultural land use. Local scale processes of ecological development are seen in the importance of time for increased organic matter and nitrogen. Basin scale processes of sediment transport and deposition are revealed by the importance of connectivity for decreases in these two elements, and by the counter-intuitive findings for nitrogen and especially phosphorous and potassium in relation to agricultural proximity. Location on a floodplain is important for differentiating development, but it cannot be reduced to univariate gradients.
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  • 55
    ISSN: 1572-9761
    Keywords: wetlands ; non-point source pollution ; nitrogen ; watershed ; leaching ; geographic information system ; landscape ; groundwater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We developed a spatially-explicit, quantitative Nitrogen Leaching Index to assess the potential for non-point source subsurface nitrogen pollution to wetlands. The index was based on the leaching potential of the watershed soils, the amount of nitrogen available for leaching, and the spatial position of nitrogen sources in the watershed. A raster or cell-based geographic information system (GIS) was used to estimate the necessary data inputs for calculating the index, such as soil hydrologic group, land use/soil type combination, groundwater residence time, and location of septic systems. The Total and Average Watershed Nitrogen Leaching Index (TWNLI and AWNLI) were calculated by summing and averaging, respectively, individual cell contributions over a watershed. Analysis of nine wetland watersheds in central New York state, USA, with mixed forest and agricultural land uses illustrated the use of the index for identifying and ranking wetlands with potential nitrogen pollution. Results showed that the spatial characteristics of a watershed potentially can effect subsurface nitrogen delivery to groundwater-dominated wetlands. The use of an index based on watershed soils, topography, and land use may be useful for assessing potential nitrogen pollution to wetlands at a regional scale.
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  • 56
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    Mangroves and salt marshes 2 (1998), S. 99-107 
    ISSN: 1572-977X
    Keywords: conductance ; mangrove ; photosynthesis ; productivity ; water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diurnal gas exchange characteristics were measured simultaneously in two mangrove species, Avicennia marina and Bruguiera gymnorrhiza, over 7 d in summer (February–March), to compare their productivity. The study was undertaken in the Beachwood Mangroves Nature Reserve, Durban, South Africa, using fully expanded leaves of young and mature trees at the top of the canopy. Gas exchange was strongly influenced by photosynthetic photon flux density (PPFD), leaf temperature and the accompanying leaf to air vapour pressure deficit (Δ w). Carbon dioxide exchange was saturated at a PPFD of about 600 μmol m-2s-1 in B. gymnorrhiza compared to 800 μmol m-2s-1 in A. marina. Maximal CO2 exchange occurred between 12h00 and 14h00 and was consistently greater in A. marina (8.8 μmol m-2s-1) than in B. gymnorrhiza (5.3 mu;mol m-2s-1). Mean internal CO2 concentrations ( ci) were 260 μl l-1 in A. marina and 252 μl l-1 in B. gymnorrhiza. Photorespiratory activity was 32% in A. marina and 30% in B. gymnorrhiza. Mean water use efficiency (WUE) was 8.0 μmol mmol-1 in A. marina and 10.6 μmol mmol-1 in B. gymnorrhiza. Diurnal leaf water potentials ranged from –0.8 to –3.5 MPa and were generally lower in A. marina.
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  • 57
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    Mangroves and salt marshes 2 (1998), S. 191-198 
    ISSN: 1572-977X
    Keywords: canopy ; Hinchinbrook ; leaf area index ; mangrove ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Data on stand structure and rates of photosynthesis were used to estimate net canopy carbon fixation and carbon accumulation as living biomass in mangrove forests in Hinchinbrook Channel, Australia. Total annual canopy net carbon fixation was estimated to be about 29 t C ha−1 yr−1. This equates to about 204,000 t C yr−1 for all mangrove forests in Hinchinbrook Channel. Of this, only about 12% was stored as living plant biomass. Although it is not yet possible to present a robust carbon balance for mangrove trees, the remainder is presumably lost through plant respiration, litter fall, root turnover and exudation of organic compounds from roots.
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    Mangroves and salt marshes 3 (1999), S. 147-153 
    ISSN: 1572-977X
    Keywords: conductance ; gas exchange ; mangrove ; photorespiration ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthetic characteristics were investigated in the geographically isolated and restricted mangrove species, P.rhizophoreae. Gas exchange measurements were made on two to seven years old hydroponically grown plants maintained in 10%, 50% and 100% seawater. CO2 exchange in the 50% and 100% seawater treatments was reduced by 10% and 26%, respectively, compared to the 10% seawater treatment. CO2 response curves indicated that carboxylation efficiency was greater in 10% than in 50% seawater, while stomatal limitation increased from 11% to 16% as salinity increased from 10% to 50% seawater. Carbon losses via photorespiration (31% and 41%) and CO2 compensation point (67 and 81 μ11−1) were greater in 50% than in the 10% seawater treatment. Maximal CO2 exchange occurred at 30 °C with no differences among the salinity treatments. The results indicate that P. rhizophoreae exhibits many gas exchange characteristics previously reported for other mangroves.
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  • 59
    ISSN: 1432-041X
    Keywords: Vitellin ; Yolk granule ; Yolk protein ; Silkworm ; Embryogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vitellin was purified from eggs of the silkworm,Bombyx mori, by a new method in which vitellin was extracted from isolated yolk granules. The purified vitellin had a molecular weight of 540,000. An antibody against purified vitellin was prepared in rabbits. It reacted with the hemolymph vitellogenin as well as with purified vitellin, but not with other proteins in the hemolymph or in the extract from yolk granules. The anti-vitellin IgG was used to immunocytochemically locate vitellin in theBombyx non-diapause egg during early developmental stages. In the egg, just after oviposition, vitellin was located in internal yolk granules and in small yolk granules of the periplasm. During the early developmental stages studied, vitellin was not metabolized uniformly throughout the egg. The vitellin of the internal yolk granules located at the posterior-dorsal part and of the small peripheral yolk granules was utilized in 16 h and 2 days, respectively, after oviposition. A thin, very vitellin-poor layer was located between the periplasm and the vitellin-rich interior in the newly laid egg. it was always in close contact with the periphery where blastoderm and germ-band cells developed.
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  • 60
    ISSN: 1432-041X
    Keywords: Major haemolymph proteins ; Development ; Cuticle ; Immunocytochemistry ; Ceratitis capitata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental profile of the major haemolymph proteins (ceratitins) inCeratitis capitata was studied. Ceratitin concentration in the haemolymph decreases dramatically during the last days of pupal life, while the amounts of ceratitins in whole organism extracts remain unchanged. By electrophoretic, immunological and immunofluorescence techniques it was revealed that ceratitins are reabsorbed by the fat body and a fraction of them is deposited in the cuticle. The possible role of ceratitins is discussed.
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  • 61
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    Trees 13 (1999), S. 138-151 
    ISSN: 0931-1890
    Keywords: Key words Cytoskeleton ; Immunocytochemistry ; Model systems ; Populus ; Secondary vascular system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Trees represent a, probably the, major component of the biosphere and have a unique place in the history of Mankind. One of their most fascinating features is the process of secondary growth which is effected principally by the secondary vascular system, the developmental continuum of secondary phloem, vascular cambium, and secondary xylem. However, for too long assumptions about the developmental biology of trees have had to be based upon studies of primary growth systems within annual, herbaceous species because study of the secondary vascular system had been largely ignored. Even when attempts are made to understand some of the most fundamental features of the secondary vascular system, such as xylogenesis, the current model system, isolated Zinnia mesophyll cells, is not entirely appropriate to the situation in the intact tree. Some deficiencies of the Zinnia system are discussed, and the advantages of the genus Populus as a model for study of the hardwood secondary vascular system are considered. Some of the new approaches which are poised to lead to significant advances in our knowledge of the cell bio-logy of the secondary vascular system of trees – spe-cifically of the cell wall, the plasmalemma, and the cytoskeleton – are discussed. The value of one of these new techniques – immunocytochemistry – is demonstrated by a consideration of recent work on the role of the cytoskeleton in the hardwood secondary vascular system.
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    Histochemistry and cell biology 106 (1996), S. 9-17 
    ISSN: 1432-119X
    Keywords: Silver enhancement ; Immunogold-silver staining ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The immunogold silver staining method (IGSS) is widely used as a sensitive and specific immunohistochemical visualisation technique. IGSS involves the specific deposition of metallic silver at the site of immunogold labelling and provides a means of visualisation at low magnification by light or electron microscopy. Silver developers for IGSS rapidly deposit metallic silver only at the site of heavy metals, including gold and silver, because of their catalytic activity. The developing solution contains the silver ions and reducing agent necessary for this reaction. Using different silver salts as ion donors and by selecting an appropriate temperature and pH, visible amounts of silver can be deposited in a few minutes at the site of colloidal gold labelling while little non-specific background deposition occurs. Inclusion of protective colloids in the solution can also be used to control the reaction. Although studies of the chemical basis of silver deposition around unlabelled colloidal gold date back to 1939, immunogold enhancement by silver was established in 1983. The IGSS method evolved from the combination of disparate photographic, histochemical and immunogold techniques which have been effectively combined and optimised over the last 10 years to provide a visualisation system which is well suited to many immunohistochemical studies.
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    Histochemistry and cell biology 106 (1996), S. 9-17 
    ISSN: 1432-119X
    Keywords: Key words Silver enhancement ; Immunogold-silver staining ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The immunogold silver staining method (IGSS) is widely used as a sensitive and specific immunohistochemical visualisation technique. IGSS involves the specific deposition of metallic silver at the site of immunogold labelling and provides a means of visualisation at low magnification by light or electron microscopy. Silver developers for IGSS rapidly deposit metallic silver only at the site of heavy metals, including gold and silver, because of their catalytic activity. The developing solution contains the silver ions and reducing agent necessary for this reaction. Using different silver salts as ion donors and by selecting an appropriate temperature and pH, visible amounts of silver can be deposited in a few minutes at the site of colloidal gold labelling while little non-specific background deposition occurs. Inclusion of protective colloids in the solution can also be used to control the reaction. Although studies of the chemical basis of silver deposition around unlabelled colloidal gold date back to 1939, immunogold enhancement by silver was established in 1983. The IGSS method evolved from the combination of disparate photographic, histochemical and immunogold techniques which have been effectively combined and optimised over the last 10 years to provide a visualisation system which is well suited to many immunohistochemical studies.
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  • 64
    ISSN: 1432-1939
    Keywords: Key words Agrostis canina ; CO2 vents ; photosynthesis ; lignification ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The aim of this study was to characterise growth and photosynthetic capacity in plants adapted to long-term contrasting atmospheric CO2 concentrations (C a). Seeds of Agrostis canina L. ssp. monteluccii were collected from a natural CO2 transect in central-western Italy and plants grown in controlled environment chambers at both ambient and elevated CO2 (350 and 700 μmol mol−1) in nutrient-rich soil. Seasonal mean C a at the source of the plant material ranged from 610 to 451 μmol CO2 mol−1, derived from C4 leaf stable carbon isotope discrimination (δ13C). Under chamber conditions, CO2 enrichment stimulated the growth of all populations. However, plants originating from elevated C a exhibited higher initial relative growth rates (RGRs) irrespective of chamber CO2 concentrations and a positive relationship was found between RGR and C a at the seed source. Seed weight was positively correlated with C a, but differences in seed weight were found to explain no more than 34% of the variation in RGRs at elevated CO2. Longer-term experiments (over 98 days) on two populations originating from the extremes of the transect (451 and 610 μmol CO2 mol−1) indicated that differences in growth between populations were maintained when plants were grown at both 350 and 700 μmol CO2 mol−1. Analysis of leaf material revealed an increase in the cell wall fraction (CWF) in plants grown at elevated CO2, with plants originating from high C a exhibiting constitutively lower levels but a variable response in terms of the degree of lignification. In vivo gas exchange measurements revealed no significant differences in light and CO2 saturated rates of photosynthesis and carboxylation efficiency between populations or with CO2 treatment. Moreover, SDS-PAGE/ LISA quantification of leaf ribulose bisphosphate carboxylase/oxygenase (Rubisco) showed no difference in Rubisco content between populations or CO2 treatments. These findings suggest that long-term adaptation to growth at elevated CO2 may be associated with a potential for increased growth, but this does not appear to be linked with differences in the intrinsic capacity for photosynthesis.
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  • 65
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; Lectin (localization) ; Phaseolus (lectin) ; Phytohemagglutinin ; Seed (lectin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined the properties and subcellular localization of phytohemagglutinin (PHA), the major lectin of the common bean (Phaseolus vulgaris.), in the axis cells of nearly mature and imbibed mature seeds. On a protein basis the axis contained about 15% as much PHA as the cotyledons. Localization of PHA was done with an indirect immunolabeling method (rabbit antibodies against PHA, followed by colloidal gold particles coated with goat antibodies against rabbit immunoglobulins) on ultra-thin cryosections which were embedded in plastic on the grids after the immunolabeling procedure. The embedding greatly improved the visualization of the subcellular structures. The small (4 nm) collodial gold particles, localized with the electron microscope, were found exclusively over small vacuoles or protein bodies in all the cell types examined (cortical parenchyma cells, vascular-bundle cells, epidermal cells). The matrix of these vacuoles-protein bodies appears considerably less dense than that of the protein bodies in the cotyledons, but the results confirm that in all parts of the embryo PHA is localized in similar structures.
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  • 66
    ISSN: 1432-2048
    Keywords: Cotyledons ; Endoplasmic reticulum ; Ferritin labeling ; Immunocytochemistry ; Phaseolus ; Protein (reserve) ; Reserve protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructure of the storage parenchyma cells of the cotyledons of developing bean (Phaseolus vulgaris L.) seeds was examined in ultrathin frozen sections of specimens fixed in a mixture of glutaraldehyde, formaldehyde and acrolein, infused with 1 M sucrose, and sectioned at-80° C. Ultrastructural preservation was excellent and the various subcellular organelles could readily be identified in sections which had been stained with uranyl acetate and embedded in Carbowax and methylcellulose. The cells contained large protein bodies, numerous long endoplasmic reticulum cisternae, mitochondria, dictyosomes, and electron-dense vesicles ranging in size from 0.2 to 1.0 μm. Indirect immunolabelling using rabbit immunoglobulin G against purified phaseolin (7S reserve protein), and ferritin-conjugated goat immunoglobulin G against rabbit immunoglobulin G was used to localize phaseolin. With a concentration of 0.1 mg/ml of anti-phaseolin immunoglobin G, heavy labeling with ferritin particles was observed ober the protein bodies, the cisternae of the endoplasmic reticulum, and the vesicles. The same structures were lightly labeled when the concentration of the primary antigen was 0.02 mg/ml. Ferritin particles were also found over the Golgi bodies. The absence of ferritin particles from other organelles such as mitochondria and from areas of cytoplasm devoid of organelles indicated the specificity of the staining, especially at the lower concentration of anti-phaseolin immunoglobulin G.
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  • 67
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; (PEP carboxylase) ; PEP carboxylase ; Sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is predominantly located in the cytoplasm of mesophyll cells.
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    Oecologia 65 (1984), S. 30-34 
    ISSN: 1432-1939
    Keywords: Stomatal conductance ; photosynthesis ; C4 grasses ; CO2xlight interaction ; light saturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The growth and photosynethetic responses to atmospheric CO2 enrichment of 4 species of C4 grasses grown at two levels of irradiance were studied. We sought to determine whether CO2 enrichment would yield proportionally greater growth enhancement in the C4 grasses when they were grown at low irradiance than when grown at high irradiance. The species studied were Echinochloa crusgalli, Digitaria sanguinalis, Eleusine indica, and Setaria faberi. Plants were grown in controlled environment chambers at 350, 675 and 1,000 μl 1-1 CO2 and 1,000 or 150 μmol m-2 s-1 photosynthetic photon flux density (PPFD). An increase in CO2 concentration and PPFD significantly affected net photosynthesis and total biomass production of all plants. Plants grown at low PPFD had significantly lower rates of photosynthesis, produced less biomass, and had reduced responses to increases in CO2. Plants grown in CO2-enriched atmosphere had lower photosynthetic capacity relative to the low CO2 grown plants when exposed to lower CO2 concentration at the time of measurement, but had greater rate of photosynthesis when exposed to increasing PPFD. The light level under which the plants were growing did not influence the CO2 compensation point for photosynthesis.
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    Journal of thermal analysis and calorimetry 53 (1998), S. 111-121 
    ISSN: 1572-8943
    Keywords: air ; cobalt dopant ; iron dopant ; n-butane ; nitrogen ; thermogravimetric analysis ; vanadium phosphorus oxides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The transformation of VOHPO4·0.5H2O (VPO) precursor doped with cobalt or iron for n-butane oxidation to maleic anhydride was investigated by thermogravimetric analysis under air and nitrogen, with and without n-butane in the flow. While almost no effect was observed in nitrogen or air, a strong influence of the doping was observed when n-butane was added to the nitrogen or air. This resulted in a delay of the decomposition of the precursor and a further reoxidation of the VPO catalyst, particularly for doping with cobalt at low percentage (1%). This shows that doping can change the oxidation state of vanadium phosphorus oxide catalysts, which can explain differences in their catalytic performances and the favourable effect of doping by cobalt.
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    Plasma chemistry and plasma processing 15 (1995), S. 279-307 
    ISSN: 1572-8986
    Keywords: Transport coefficients ; transport properties ; viscosity ; thermal conductivity ; electrical conductivity ; diffusion coefficient ; Chapman-Etskog method ; air ; argon ; nitrogen ; oxygen ; plasma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract Calculated values of the viscosity, thermal conductivity and electrical conductivity of air and mixtures of air and argon, air and nitrogen, and air and oxygen at high temperatures are presented. In addition, combined ordinary, pressure, and thermal diffusion coefficients are given for the gas mixtures. The calculations, which assione local thermodynamic equilibrium, are performed for atmospheric pressure plasmas in the temperature range from 300 to 30,000 K. The results for air plasmas are compared with those of published theoretical and experimental studies. Significant discrepancies are found with the other theoretical studies; these are attributed to differences in the collision integrals used in calculating the transport coefficients. A number of the collision integrals used here are significantly more accurate than values used previously, resulting in more reliable values of the transport coefficients.
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    Plasma chemistry and plasma processing 16 (1996), S. 577-603 
    ISSN: 1572-8986
    Keywords: Cathode erosion ; electrode materials ; argon ; nitrogen ; hydrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract Cathode erosion continues to be a problem hindering the widespread application of plasma technology. In this work, cathode erosion was studied on titanium, stainless steel 314, copper-nickel 10% and 30%, and copper 122 for magnetically rotated arcs operating in argon, nitrogen, and argon/hydrogen mixtures at a constant magnetic flux density of 0.1 T Titanium and stainless steel gave very low erosion rates in argon (0.2 and 0.3, μg/C respectively). Cupronickels were shown to be suitable for nitrogen and hydrogen plasmas. The slope of hydrogen solubility versus temperature in the cathode material was found to be important in determining hydrogen plasma erosion characteristics. When the plasma gas has a high solubility in the cathode material, or can react with the cathode, a negative erosion rate may result. When gas solubility in the cathode is low, oxide stability and mode of electron emission may govern the erosion rate. A high gas solubility in the cathode material, as with hydrogen, can result in mechanical erosion due to micro-explosions near the cathode surface.
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    Water, air & soil pollution 85 (1995), S. 677-682 
    ISSN: 1573-2932
    Keywords: soils ; acidification ; ancient woodland ; nitrogen ; environmental change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This study outlines the results of analysis of soil samples collected from fixed quadrats located in a regular pattern across Wytham Wood. Oxfordshire, England. The site contains plots of mixed deciduous ancient woodland and more recent plantations. A previous soil study was undertaken in 1974 and samples archived. Soils were resampled in 1991 and some re-analysis of the 1974 samples was undertaken. Soils were of a wide range in types from sands to gravels with a pH range of 3.0–7.0. Results showed some decline in pH in lower horizons, but most striking was a large increase in soil nitrogen for all horizons and soil types.
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  • 73
    ISSN: 1573-2932
    Keywords: air pollution ; sulphur ; nitrogen ; base cations ; throughfall ; Scots pine ; needle elements ; soil leachate ; N.-W. Russia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Bulk precipitation and throughfall analyses in 50–100-year-old Scots pine stands revealed decreasing sulphur, nitrogen, calcium and magnesium deposition gradients, which extend from the St. Petersburg-Leningrad region and N.-E. Estonia to S.-E. Finland. The Ca and Mg deposition alleviate the acidifying effect of sulphur and nitrogen. The Scots pine canopies acted as a sink for ammonium and nitrate, while the canopy interactions increased sulphur, calcium and magnesium content in throughfall. Foliar S, N and Ca concentrations correlated positively with the corresponding deposition loads. In contrast, low foliar magnesium concentrations were detected in the vicinity of St. Petersburg. The results indicate that the sulphur and calcium deposition may have increased soil leachate S and Ca concentrations in the most polluted Scots pine stands.
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    Journal of materials synthesis and processing 7 (1999), S. 311-319 
    ISSN: 1573-4870
    Keywords: Iron ; carbon ; nitrogen ; microstructure ; characterization ; HIP-drip
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A new processing technique makes nitrogen alloying possible by adding nitrogen under elevated nitrogen pressure to prealloyed Fe-C ingots during continuous casting, producing a whole new class of precipitation-free, iron–carbon–nitrogen alloys. When both carbon and nitrogen bulk concentration levels exceeded 0.5 wt%, a duplex fcc-/(bcc-bct-) Fe microstructure resulted that is iron carbide- and nitride-free. With increasing carbon and nitrogen concentrations, there was an increase in the retained fcc-Fe phase. In cooling rate studies, increasing carbon and nitrogen concentrations shifted the knee of the fcc-Fe-to-bcc-Fe phase time–temperature–transformation (T–T–T) curve to longer times. Hardness, compression strength, and wear resistance increased with increasing carbon and nitrogen concentrations and were superior to iron–carbon alloys without the nitrogen addition.
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    Environmental and resource economics 10 (1997), S. 341-362 
    ISSN: 1573-1502
    Keywords: Baltic Sea ; eutrophication ; nitrogen ; phosphorus ; cost effective
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Economics
    Notes: Abstract Due to eutrophication caused by heavy loads of nitrogen and phosphorus, the biological conditions of the Baltic Sea have been disturbed: large sea bottom areas without any biological life, low stocks of cods, and toxic blue green algaes. It is recognized that the nitrogen and phosphorus loads to the Baltic Sea must be reduced by 50% in order to restore the sea. The main purpose of this paper is to calculate cost effective nitrogen and phosphorus reductions to the Baltic Sea from the nine countries surrounding the Baltic Sea. The results show a significant difference in minimum costs of decreasing nitrogen and phosphorus loads to the Sea: approximately 12 000 millions of SEK per year and 3 000 millions of SEK respectively for reductions by 50%. It is also shown that a change from a policy of cost-effective nutrient reductions to a policy where each country reduces the nutrient loads by 50% increase total costs for both nitrogen and phosphorus reductions by about 300%. The results are, however, sensitive to several of the underlying assumptions and should therefore be interpreted with much caution.
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  • 76
    ISSN: 1573-1561
    Keywords: Piperidine alkaloids ; pinidine ; euphococcinine ; foliar chemistry ; nitrogen ; Pinus ponderosa ; Pinus contorta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We quantified 2,6-disubstituted piperidine alkaloids in Pinus ponderosa and P. contorta needles from three forest sites in April, June, August, and December. Alkaloids were detected on at least one date in 71% of the ponderosa pine and in 29% of the lodgepole pine trees sampled. Pinidine was the major alkaloid constituent of ponderosa pine, while euphococcinine was the predominant compound in lodgepole pine. For ponderosa pine, total alkaloid concentrations were very low at two sites on all dates. At the third site, concentrations were variable but significantly higher on all dates. Total alkaloid concentrations in previous-year foliage from this site were highest in April, then significantly lower from June through December. Current-year foliage collected in August and December had significantly higher alkaloid concentrations than previous-year foliage on the same dates. Variation in foliar nitrogen concentrations accounted for some of the alkaloid variation in current-year foliage sampled in August.
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    Water, air & soil pollution 85 (1995), S. 623-628 
    ISSN: 1573-2932
    Keywords: monitoring ; deposition ; sulphur ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The total deposition of sulphur (S) and nitrogen (N) components in Norway during the period 1988–1992 has been estimated on the basis of measurement data of air- and precipitation chemistry from the national monitoring network. There are large regional variations in depositions with highest values in the southwestern part of Norway. Time series analysis of annual mean concentrations of sulphur dioxide (SO2) and sulphate (SO4 −−) in air, non marine SO4 −−, nitrate (NO3 −) and ammonium (NH4 +) in precipitation, shows a significant reduction in the S concentrations both in air and precipitation. In precipitation the concentrations are reduced by 30–45 percent in Southern Norway and 45–55 percent in Central and Northern Norway. Even larger reductions are observed in air concentrations with 50–65 percent reduction in Southern Norway and 65–88 percent reduction further north. For N components there are generally no significant trends in concentration levels nor in precipitation or air. The observed trends are comparable with reported trends in emission.
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    Water, air & soil pollution 85 (1995), S. 1613-1622 
    ISSN: 1573-2932
    Keywords: Deposition ; ecosystem ; nitrogen ; Norway spruce ; nutrient cycling ; production ; root function ; sulphur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In this paper we try to interpret results from different investigations where an ecosystem with Norway spruce was manipulated with increased N and S deposition via the soil system. The site, in Skogaby in Southwest Sweden, had 1989–93 an annual deposition of 9 kg NH4-N; 7 kg NO3-N and 20 kg SO4-S ha−1. The stand was treated during 6 years with 100 kg N and 114 kg S ha− y−1 in the form of ammonium sulphate (NS treatment). The stand reacted with increased above ground production of 31% after 3 years of treatment. The uptake above ground of N was 155 kg ha−1 higher than in the control. Those trends were even stronger after 6 years of treatment. There were no decreases in the uptake of P, K, Ca or Mg (but for B) after 3 or 6 years of NS-treatment. Needle macro nutrient concentrations in relation to N decreased for several nutrients due to dilution effects. As result of the NS treatment pH increased markedly in the litter layer, and less, but significantly, in the humus layer. A decrease in pH value by about 0.3 units was found in the rest of the soil profile down to 50 cm. Dry mass of needle litter fall and litter layer both increased as a result of 6 years of NS-treatment. After three years of treatment 77–80% of all living fine roots in both control and NS treatment were found in the humus layer and the upper 10 cm of the mineral soil. The amount of living fine roots in the humus layer of NS-treated trees decreased to about one third of the control, and the amount of dead fine roots increased by 150% compared with untreated trees after 6 years of treatment. It is argued that the decreased amount of living and increased amount of dead fine roots not necessarily are indications of decreased root vitality. It can also be explained by increased root turnover rate and decreased decomposition rates of N rich new and old fine root litter. No inorganic N was leached from the control plots whereas the NS treated plots started to leach NO3 the second year of treatment. During 1989–1993 a total of 44 kg NO3-N and 30 kg NH4-N per ha was lost from the system which means that 88% of the N supplied was retained by the ecosystem. At first SO4 was adsorbed in the soil, but after five years of treatment the output was almost equal to the input.
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    Water, air & soil pollution 85 (1995), S. 15-24 
    ISSN: 1573-2932
    Keywords: Acid deposition ; global change ; Asia ; fertilizer ; nitrogen ; sulfur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Most acid-deposition investigations have been concerned with the impact of nitrogen oxides (NOx) and sulfur dioxide (SO2) emissions on Europe and North America. This paper examines three issues beyond this central focus. Major conclusions are 1) ammonia (NH3) emissions and subsequent nitrogen (N) accumulation in terrestrial ecosystems have the potential to generate significant acidification in terrestrial and aquatic ecosystems; 2) sulfur (S) and N accumulation in environmental reservoirs will not only result in significant and extensive acidification but will also impact the earth's radiation balance, tropospheric oxidizing capacity, ecosystem nutrient balance and groundwater quality; and 3) future emissions will substantially increase in the developing world, especially in Asia. By 2020, Asian emissions of SO2, NOx and NH3 will be equal to or greater than the combined emissions from Europe and North America.
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    Water, air & soil pollution 85 (1995), S. 101-110 
    ISSN: 1573-2932
    Keywords: carbon ; nitrogen ; sulfur ; biogeochemistry ; mitigation ; global change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Forest systems cover more than 4.1×109 ha of the Earth's land area. The future response and feedbacks of forest systems to atmospheric pollutants and projected climate change may be significant. Boreal, temperate and tropical forest systems play a prominent role in carbon (C), nitrogen (N) and sulfur (S) biogeochemical cycles at regional and global scales. The timing and magnitude of future changes in forest systems will depend on environmental factors such as a changing global climate, an accumulation of CO2 in the atmosphere, and increase global mineralization of nutrients such as N and S. The interactive effects of all these factors on the world's forest regions are complex and not intuitively obvious and are likely to differ among geographic regions. Although the potential effects of some atmospheric pollutants on forest systems have been observed or simulated, large uncertainty exists in our ability to project future forest distribution, composition and productivity under transient or nontransient global climate change scenarios. The potential to manage and adapt forests to future global environmental conditions varies widely among nations. Mitigation practices, such as liming or fertilization to ameliorate excess NOx or SOx or forest management to sequester CO2 are now being applied in selected nations worldwide.
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  • 81
    ISSN: 1573-2932
    Keywords: forest soil ; leaching ; lysimeter ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Three years of N application to a Cambic arenosol (Typic Udorthent) in two lysimeter series, one with and one without young saplings of Pinus sylvestris L. have produced significant changes in soil solution and leachate chemistry. An application of 30 kg N/ha*yr−1 significantly increased NO3 − leaching from the soil. This N load was also sufficient to significantly increase the mobility of the phyto-toxic elements Al3+ and Mn2+, likewise to increase leaching of the important plant nutrients Ca2+, Mg2+ and K+. At a N load of 90 kg N/ha*yr−1 significant increase in NH4 + leaching was observed, but total leaching of NH4 + was still very low compared to NO3 − leaching. No significant treatment effects were found for SO4 2−, Fe2+ and Cl− in the leachate. Trees grown in the lysimeters buffered the acidifying effect of N application and increased the leachate pH by 0.2 pH units compared to lysimeters without trees.
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  • 82
    ISSN: 1573-2932
    Keywords: sitka spruce ; nitrogen ; deposition ; leaching ; proton production ; green spruce aphid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Studies of biogeochemical cycling and soil acidification have been carried out in even aged stands of Norway spruce, sitka spruce, Douglas fir, beech and oak under the frame of “The Element Cycling Project”. Deposition of excess nitrogen to forests is important as a potential acidifying input. In Denmark, reduced vitality in Norway spruce has promoted extensive planting of sitka spruce. However, several spruce aphid infestations have caused defoliation in many sitka spruce stands. The objectives of this study were to evaluate the effects of deposition and increased litterfall due to spruce aphid infestations on nitrogen transformations in the forest floor in sitka spruce stands on different soil types. The deposition of throughfall nitrogen range from 19 to 35 kg/ha/year. Fluxes of nitrogen in litterfall ranged from 21 to 77 kg/ha/year, whereas nitrogen leaching range from 1 to 57 kg/ha/year. Leaching was lowest at the infertile sites, but increased with magnitude of deposition and aphid infestations. Proton production according to the nitrogen transformations was largest at the fertile site most often affected by infestations. Huge amounts of bird droppings, honey dew and input of easily available nutrients by canopy leaching probably induced litter decomposition and formation of NO 3 − in the soil water.
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  • 83
    ISSN: 1573-2932
    Keywords: atmospheric deposition ; high elevation forests ; foliar uptake ; cloudwater ; nitrogen ; sulphur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract From 1986–1989, a team of scientists measured atmospheric concentrations and fluxes in precipitation and throughfall, and modeled dry and cloudwater deposition in a spruce-fir forest of the Great Smoky Mountains National Park which is located in the Southern Appalachian Region of the United States. The work was part of the Integrated Forest Study (IFS) conducted at 12 forests in N. America and Europe. The spruce-fir forest at 1740 m consistently received the highest total deposition rates (∼2200, 1200, and 700 eq ha−1 yr−1 for SO4 2−, NO3 −, and NH4 +). During the summers of 1989 and 1990 we used multiple samplers to measure hydrologie, SO4 2−, and NO3 − fluxes in rain and throughfall events beneath spruce forests above (1940 m) and below (1720 m) cloud base. Throughfall was used to estimate total deposition using relationships determined during the IFS. Although the SO4 2− fluxes increased with elevation by a factor of ∼2 due to higher cloudwater interception at 1940 m, the NO3 − fluxes decreased with elevation by ∼30%. To investigate further, we began year round measurements of fluxes of all major ions in throughfall below spruce-fir forests at 1740 m and at 1920 m in 1993–1994. The fluxes of most ions showed a 10–50% increase with elevation due to the ∼70 cm yr−1 cloudwater input at 1920 m. However, total inorganic nitrogen exhibited a 40% lower flux in throughfall at 1920 m than at 1740 m suggesting either higher dry deposition to trees at 1740 m or much higher canopy uptake of nitrogen by trees at 1920 m. Differential canopy absorption of N by trees at different elevations would have significant consequences for the use of throughfall N fluxes to estimate deposition. We used artificial trees to understand the foliar interactions of N.
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  • 84
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    Water, air & soil pollution 85 (1995), S. 1765-1770 
    ISSN: 1573-2932
    Keywords: nitrogen ; sulphur ; input ; forested catchment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The study covers 1991–1994 concentrations of SO2 and NO2 in the air, concentrations of sulphur and nitrogen in bulk precipitation, throughfall and stemflow as well as input of S and N to the Ratanica forested catchment (S. Poland), which is exposed to moderate anthropogenic pollution are presented. There was high input of sulphur (26 kg ha−1) and nitrogen (24 kg ha−1) to the catchment, mainly in NH4+ (18 kg ha−1). The significant contribution of NH4 + connected with intensive agriculture in surrounding fields has led to eutrophication of the ecosystem.
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  • 85
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    Water, air & soil pollution 85 (1995), S. 1461-1466 
    ISSN: 1573-2932
    Keywords: ozone ; wheat ; Triticum aestivum ; growth ; senescence ; biomass partitioning ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In closed-chamber fumigation experiments dry matter partitioning and chlorophyll fluorescence of wheat were studied, analysing the effects of ozone during different stages of plant development. Ozone causes enhanced leaf senescence, leading to a loss of green leaf area and, consequently to a decreased supply of assimilates, affecting (in increasing order of severeness) stem, ear and grain productivity because of reduced storage pools for translocation. Leaves of plants before shooting stage were most sensitive but the lack of green leaf area after ear emergence had the most pronounced effects on grain yield. Measurements of photochemical capacity showed that evidence for negative ozone effects could be found in changes of chlorophyll fluorescence parameters in leaf sections not yet showing visible ozone injury. Negative effects on photosynthesis were more distinct with increasing accumulated ozone dose, with increasing age of leaf tissue and with increasing ozone sensitivity of the cultivar. The changes in chlorophyll fluorescence are most likely to be explained by a decreased pool size of plastoquinones caused by ozone.
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  • 86
    ISSN: 1573-2932
    Keywords: atmospheric deposition ; ecosystem ; hydrology ; nitrogen ; sulfur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Information on atmospheric inputs, water chemistry and hydrology were combined to evaluate elemental mass balances and assess temporal changes in elemental transport from 1983 through 1992 for the Arbutus Lake watershed. This watershed is located within a northern hardwood ecosystem at the Huntington Forest within the central Adirondack Mountains of New York (USA). Changes in water chemistry, including increasing NO3 − concentrations (1.1 μmol c , L−1 yr-1), have been detected during this study period. Starting in 1991 hydrological flow has been measured from Arbutus Lake and these measurements were compared with predicted flow using the BROOK2 hydrological simulation model. The model adequately (r2=0.79) simulated flow from this catchment and was used to estimate drainage for earlier periods when direct hydrological measurements were not available. Modeled drainage water losses coupled with estimates of wet and dry atmospheric deposition were used to calculate solute budgets. Export of SO4 2− (831 mol c ha−1 yr−1) from the greater Arbutus Lake watershed exceeded estimates of atmospheric deposition in an adjacent hardwood stand suggesting an additional source of S. These large drainage losses of SO4 2− also contributed to the drainage fluxes of basic cations (Ca2+, Mg2+, K+ and Na+). Most of the atmospheric inputs of inorganic N were retained (average of 74% of wet precipitation and 85% total deposition) in the watershed. There were differences among years (56 to 228 mol ha−1 yr−1) in drainage water losses of N with greatest losses occurring during a warm, wet period (1989–1991).
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  • 87
    ISSN: 1573-2932
    Keywords: Pulp and paper mill sludge ; nitrogen ; DOC ; heavy metals ; water quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Primary sludge, secondary sludge, and wood ash from a pulp and paper mill were combined with sand to create a synthetic topsoil (C:N ratio of 18:1) to restore an abandoned gravel pit. Synthetic topsoil was applied to field microcosms at rates equivalent to 0, 2170, 4341, or 6511 kg N/ha; each was seeded with grass. Fifteen chemical constituents in leachate were measured during two field seasons. Cadmium, Ni and Zn were mobilized rapidly by soil disturbance. Chloride and SO4-S eluted rapidly from the sludge along with Na. Nitrate leached with Ca late in each field season when sludge N-mineralization and nitrification exceeded plant uptake and microbial immobilization. Ammonium elution was negligible. Dissolved organic carbon (DOC) was mobilized by decomposition of organic matter in the sludge, as were Mg and K. Copper eluted with DOC, probably as an organic ligand. Lead and ortho-P were below our detection limits. We concluded that a synthetic topsoil with a 30:1 C:N ratio applied at a rate of 2100-4300 kg N/ha should provide adequate plant nutrition while minimizing water quality hazards.
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  • 88
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    Water, air & soil pollution 99 (1997), S. 477-486 
    ISSN: 1573-2932
    Keywords: sediment ; nitrogen ; phosphorus ; organic matter ; cluster analysis ; Gulf of Finland ; estuaries
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Dry weight (DW), ignition loss (IL) and concentrations of total nitrogen (TN) and total phosphorus (TP) of the sediment surface layer (0 to 10 cm, 1 cm slices) were analyzed from 20 sites in the eastern Gulf of Finland. The distance of the sampling sites from the mouth of the River Neva explained the nutrient concentrations of the sediments well, while the effect of water depth was negligible. The increase of TN and the decrease of TP along the transect from the river mouth towards the open Gulf were caused by the diminishing share of allochthonous material supplied from the River Neva. The mean TN concentration of the different accumulation areas was about 40 % higher in the sediment surface than in the deeper layer (9 to 10 cm). The corresponding difference for TP varied from 53 to 56 %. The results suggest considerable netflux of nutrients from sediment to water. The net sediment accumulation of nutrients were estimated as 6.0 g m-2 a-1 of N and 1.7 g m-2 a-1 of P corresponding 22 000 t a-1 of N and 6 100 t a-1 of P for the whole eastern Gulf.
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  • 89
    ISSN: 1573-2932
    Keywords: Adriatic Sea ; nutrients ; benthic fluxes ; carbon ; nitrogen ; silicon ; phosphorus ; budgets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Benthic fluxes of dissolved inorganic N, Si and P nutrients, alkalinity, dissolved inorganic C (DIC), and O2 from sediments in the Gulf of Trieste (northern Adriatic, Italy) were measured monthly in the period September 1995 – August 1996 using in situ incubated light benthic chambers. The highest efluxes of DIC, NH4 +, PO4 3−, Si(OH)4, and NO3 − influxes encountered in late summer — early autumn were the consequence of degradation of benthic microalgae, and in autumn mostly of sedimented phytoplankton. High NO3 − efflux was observed in spring. Only NH4 + and Si(OH)4 fluxes were significantly correlated with temperature. This correlation suggests that the rate of downward input and the quality and quantity of sedimentary organic matter (autochthonous and allochthonous) were superimposed on the temperature fluctuations. High DIC, NH4 + and Si(OH)4 effluxes observed in July 1996 were due to the late spring — early summer degradation of sedimentary organic matter produced by benthic microalgae, while the autumn phytoplankton bloom was quickly reflected in enhanced benthic fluxes due to higher temperature. Significant correlations between NH4 +, PO4 3− and Si(OH)4 fluxes suggested their parallel regeneration and utilization at the sediment-water interface. The nutrient fluxes were linked to O2 consumption, suggesting that aerobic oxidation processes were important at the sediment-water interface in the Gulf. The N, P and Si nutrients released from sediment pore waters are probably utilized in benthic microalgal and bottom-water primary production. This indicates that pelagic and benthic communities in the central part of the Gulf of Trieste function relatively independently of each other.
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  • 90
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    Water, air & soil pollution 98 (1997), S. 389-399 
    ISSN: 1573-2932
    Keywords: fertilizer ; nitrogen ; pollution ; runoff ; stable isotopes ; sugarcane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In many forested wetlands of Louisiana, surface water quality is being deteriorated by nutrient input from adjacent agricultural production area. This field study assesses the input of fertilizer N, applied to sugarcane fields, to forested wetlands. The potential use of natural abundance variations in15N/14N ratios for identification and tracing surface water N sources (NH 4 + - and NO 3 − -N) was evaluated. Runoff and surface water samples were collected from sugarcane fields and bordering forested wetlands (6 stations) over a 16 month period and analyzed for NH 4 + -N, NO 3 − -N, and associated NH 4 + -δ 15N and NO 3 − -δ 15N ratios. FertilizerN draining into adjacent forested wetland was estimated to be only a small fraction of the amount applied. Concentrations of NH 4 + - and NO 3 − -N in the collected water samples were low and ranged from 0.02 to 1.79 mg L−1. Isotopic analysis revealed NH 4 + -δ 15N and NO 3 − -δ 15N means were distinctive and may have the potential to be used as tracers of N contamination. The mean NH 4 + -δ 15N value was +18.6±7.1‰ and the NO 3 − -δ 15N mean was +8.3±3.1‰. Anomalously high NO 3 − -δ 15N values (〉30‰) were attributed to denitrification.
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  • 91
    ISSN: 1573-5028
    Keywords: Cell cycle ; circadian clock ; green alga ; GTP-binding proteins ; light regulation ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ADP-ribosylation factor (ARF) is a highly conserved, low molecular mass (ca. 21 kDa) GTP-binding protein that has been implicated in vesicle trafficking and signal transduction in yeast and mammalian cells. However, little is known of ARF in plant systems. A putative ARF polypeptide was identifed in subcellular fractions of the green alga Chlamydomonas reinhardtii, based on [32P]GTP binding and immunoblot assays. A cDNA clone was isolated from Chlamydomonas (Arf1), which encodes a 20.7 kDa protein with 90% identity to human ARF1. Northern blot analyses showed that levels of Arf1 mRNA are highly regulated during 12 h/12 h light/dark (LD) cycles. A biphasic pattern of expression was observed: a transient peak of Arf1 mRNA occurred at the onset of the light period, which was followed ca. 12 h later by a more prominent peak in the early to mid-dark period. When LD-synchronized cells were shifted to continuous darkness, the dark-specific peak of Arf1 mRNA persisted, indicative of a circadian rhythm. The increase in Arf1 mRNA at the beginning of the light period, however, was shown to be light-dependent, and, moreover, dependent on photosynthesis, since it was prevented by DCMU. We conclude that the biphasic pattern of Arf1 mRNA accumulation during LD cycles is due to regulation by two different factors, light (which requires photosynthesis) and the circadian clock. Thus, these studies identify a novel pattern of expression for a GTP-binding protein gene.
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  • 92
    ISSN: 1573-5028
    Keywords: gene expression ; photosynthesis ; protein turnover ; psbA ; tac promoter ; D1 protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Over-expression of the psbAIII gene encoding for the D1 protein (form II; D1:2) of the photosystem II reaction centre in the Synechococcus sp. PCC 7942 was studied using a tac promoter and the lacI Q system. Over-expression was induced with 40 μg/ml IPTG in the growth medium for either 6 or 12 h at growth irradiance (50 μmol photons m-2 s-1). This treatment doubled the amount of psbAII/III mRNA and the D1:2 protein in membranes but decreased the amount of psbAI messages and the D1:1 protein. The total amount of both heterodimeric reaction centre proteins, D1 and D2, remained constant under growth light conditions, indicating that the number of PSII centres in the membranes was not affected, only the form of the D1 protein was changed from D1:1 to D1:2 in most centres. When the cells were photoinhibited either at 500 or 1000 μmol photons m-2 s-1, in the presence or absence of the protein synthesis inhibitor lincomycin, the D1:2 protein remained at a higher level in cells in which over-expression had been induced by IPTG. These cells were also less prone to photoinhibition of PSII. It is suggested that the tolerance of cells to photoinhibition increases when most PSII reaction centres contain the D1:2 protein at the beginning of high irradiance. This tolerance is further strengthened by maintaining psbAIII gene over-expression during the photoinhibitory treatment.
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  • 93
    ISSN: 1573-5028
    Keywords: antisense ; Corynebacterium glutamicum ; Escherichia coli ; Flaveria trinervia ; overexpression ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phosphoenolpyruvate carboxylase (PEPC) genes from Corynebacterium glutamicum (cppc), Escherichia coli (eppc) or Flaveria trinervia (fppc) were transferred to Solanum tuberosum. Plant regenerants producing foreign PEPC were identified by Western blot analysis. Maximum PEPC activities measured in eppc and fppc plants grown in the greenhouse were doubled compared to control plants. For cppc a transgenic plant line could be selected which exhibited a fourfold increase in PEPC activity. In the presence of acetyl-CoA, a known activator of the procaryotic PEPC, a sixfold higher activity level was observed. In cppc plants grown in axenic culture PEPC activities were even higher. There was a 6-fold or 12-fold increase in the PEPC activities compared to the controls measured in the absence or presence of acetyl-CoA, respectively. Comparable results were obtained by transient expression in Nicotiana tabacum protoplasts. PEPC of C. glutamicum (PEPC C.g.) in S. tuberosum leaf extracts displays its characteristic K m(PEP) value. Plant growth was examined with plants showing high expression of PEPC and, moreover, with a plant cell line expressing and antisense S. tuberosum (anti-sppc) gene. In axenic culture the growth rate of a cppc plant cell line was appreciably diminished, whereas growth rates of an anti-sppc line were similar or slightly higher than in controls. Malate levels were increased in cppc plants and decreased in antisense plants. There were no significant differences in photosynthetic electron transport or steady state CO2 assimilation between control plants and transformants overexpressing PEPC C.g. or anti-sppc plants. However, a prolonged dark treatment resulted in a delayed induction of photosynthetic electron transport in plants with less PEPC. Rates of CO2 release in the dark determined after a 45 min illumination period at a high proton flux density were considerably enhanced in cppc plants and slightly diminished in anti-sppc plants. When CO2 assimilation rates were corrected for estimated rates of mitochondrial respiration in the light, the electron requirement for CO2 assimilation determined in low CO2 was slightly lower in transformants with higher PEPC, whereas transformants with decreased PEPC exhibited an appreciably elevated electron requirement. The CO2 compensation point remained unchanged in plants (cppc) with high PEPC activity, but might be increased in an antisense plant cell line. Stomatal opening was delayed in antisense plants, but was accelerated in plants overexpressing PEPC C.g. compared to the controls.
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  • 94
    ISSN: 1573-5028
    Keywords: photosystem II ; photosynthesis ; chlorophyll-binding protein ; Synechocystis ; oxygen evolution ; oligonucleotide-mediated mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract CP 47, a component of photosystem II (PSII) in higher plants, algae and cyanobacteria, is encoded by the psbB gene. Site-specific mutagenesis has been used to alter a portion of the psbB gene encoding the large extrinsic loop E of CP 47 in the cyanobacterium Synechocystis 6803. Alteration of a lysine residue occurring at position 321 to glycine produced a strain with altered PSII activity. This strain grew at wild-type rates in complete BG-11 media (480 µM chloride). However, oxygen evolution rates for this mutant in complete media were only 60% of the observed wild-type rates. Quantum yield measurements at low light intensities indicated that the mutant had 66% of the fully functional PSII centers contained in the control strain. The mutant proved to be extremely sensitive to photoinactivation at high light intensities, exhibiting a 3-fold increase in the rate of photoinactivation. When this mutant was grown in media depleted of chloride (30 µM chloride), it lost the ability to grow photoautotrophically while the control strain exhibited a normal rate of growth. The effect of chloride depletion on the growth rate of the mutant was reversed by the addition of 480 µM bromide to the chloride-depleted BG-11 media. In the presence of glucose, the mutant and control strains grew at comparable rates in either chloride-containing or chloride-depleted media. Oxygen evolution rates for the mutant were further depressed (28% of control rates) under chloride-limiting conditions. Addition of bromide restored these rates to those observed under chloride-sufficient conditions. Measurements of the variable fluorescence yield indicated that the mutant assembled fewer functional centers in the absence of chloride. These results indicate that the mutation K321G in CP 47 affects PSII stability and/or assembly under conditions where chloride is limiting.
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  • 95
    ISSN: 1573-5028
    Keywords: chloroplasts ; gene expression ; heat bleaching ; photosynthesis ; transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A shift in the ratio of chlorophyll (Chl) a and Chl b is an early indicator of heat bleaching in Euglena gracilis. This observation prompted us to consider whether or not changes in steady-state levels of chloroplast transcripts and in transcriptional activity could limit the synthesis of Chl a-binding proteins in bleaching plastids. We found that the mature transcripts for CP47 and CP43, the Chl a-binding apoproteins of the proximal antenna of photosystem II, decline sharply very early during bleaching. Our study also shows that transcription of psbB and psbC, the chloroplast genes encoding CP47 and CP43, remains essentially unchanged during the same interval. We conclude that posttranscriptional events, such as mRNA stability, could play a major role in initiating an irreversible loss of chloroplast function in Euglena at a moderately elevated temperature. Lack of these transcripts would eventually impair the assembly of photosystem II in thylakoids.
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  • 96
    ISSN: 1573-5028
    Keywords: Cyanobacteria ; gene copy number ; light regulation ; photosynthesis ; photosystem II reaction center ; polymerase chain reaction ; psbA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA sequence, copy number, expression and phylogenetic relevance of the psbA gene from the abundant marine prokaryote P. marinus CCMP 1375 was analyzed. The 7 amino acids near the C-terminus missing in higher plant and in Prochlorothrix hollandica D1 proteins are present in the derived amino acid sequence. P. marinus contains only a single psbA gene. Thus, this organism lacks the ability to adapt its photosystem II by replacement of one type of D1 by another, as several cyanobacteria do. Phylogenetic trees suggested the D1-1 iso-form from Synechococcus PCC 7942 as the next related D1 protein and place P. Marinus separately from Prochlorothrix hollandica among the cyanobacteria.
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  • 97
    ISSN: 1573-5028
    Keywords: chlorophyll synthesis ; cyanobacteria ; chlorophyl-binding proteins ; photosynthesis ; thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Part of the chlL gene encoding a component involved in light-independent protochlorophyllide reduction was deleted in wild type and in a photosystem I-less strain of Synechocystis sp. PCC 6803. In resulting mutants, chlorophyll biosynthesis was fully light-dependent. When these mutants were propagated under light-activated heterotrophic growth conditions (in darkness except for 15 min of weak light a day) for several weeks, essentially no chlorophyll was detectable but protochlorophyllide accumulated. Upon return of the chlL - mutant cultures to continuous light, within the first 6 h chlorophyll was synthesized at the expense of protochlorophyllide at a rate independent of the presence of photosystem I. Chlorophyll biosynthesized during this time gave rise to a 685 nm fluorescence emission peak at 77 K in intact cells. This peak most likely originates from a component different from those known to be directly associated with photosystems II and I. Development of 695 and 725 nm peaks (indicative of intact photosystem II and photosystem I, respectively) required longer exposures to light. After 6 h of greening, the rate of chlorophyll synthesis slowed as protochlorophyllide was depleted. In the chlL - strain, greening occurred at the same rate at two different light intensities (5 and 50 μE m-2s-1), indicating that also at low light intensity the amount of light is not rate-limiting for protochlorophyllide reduction. Thus, in this system the rate of chlorophyll biosynthesis is limited neither by biosynthesis of photosystems nor by the light-dependent protochlorophyllide reduction. We suggest the presence of a chlorophyll-binding ‘chelator’ protein (with 77 K fluorescence emission at 685 nm) that binds newly synthesized chlorophyll and that provides chlorophyll for newly synthesized photosynthetic reaction centers and antennae.
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  • 98
    ISSN: 1573-5028
    Keywords: photosynthesis ; photosystem II ; chloroplast genes ; thylakoid polypeptides ; Chalamydomonas reinhardii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The region of the chloroplast genome of Chlamydomonas reinhardii containing the gene of the thylakoid polypeptide D2 (psbD) has been sequenced. A unique open reading frame of 350 codons exists in this region. Because the first ATG is followed 11 codons downstream by a second one, the D2 polypeptide consists of either 339 or 350 amino acids. Comparison of the sequences of D2 and the 32K dalton polypeptides, both of which are associated with photosystem II, reveals partial homology. Although, the overall homology of these two polypeptides is only 27%, they contain several related regions and their hydropathic profiles are strikingly similar. These data suggest that the two polypeptides may have related functions and/or that their genes may have originated from a common ancestor. Alternatively, convergent evolution of these polypeptides may be due to structural constraints in the thylakoid membrane. Limited sequence homology is also observed between the D2 polypeptide and some of the subunits of the reaction centers of photosynthetic bacteria.
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  • 99
    ISSN: 1573-5028
    Keywords: Cyanobacteria ; photosynthesis ; random mutagenesis ; sodium bisulfite ; thylakoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To identify amino acid residues of the D2 protein that are critical for functional photosystem II (PS II), sodium bisulfite was utilized for in vitro random mutagenesis of the psbDI gene from Synechocystis sp. PCC 6803. Sodium bisulfite reacts specifically with cytosine in single-stranded regions of DNA and does not attack double-stranded DNA. Using a hybrid plasmid that was single-stranded in the region to be mutagenized and that was double-stranded elsewhere, mutations were targeted to a specific psbDI region coding for the lumenal A-B loop of the D2 protein. Several mutants were isolated with a total of 15 different amino acid changes in the loop. The majority of these mutations did not result in a loss of photoautotrophic growth or in significantly altered PS II function. However, mutation of Glu-69 to Lys, Ser-79 to Phe, and Ser-88 to Phe were found to influence photosystem II activity; the importance of the latter two residues for proper PS II function was unexpected. Cells carrying the double mutation S79F/S88F in D2 did not grow photoautotrophically and had no functionally active PS II centers. The single mutant S79F was also incapable of photoautrophic growth, but displayed reasonably stable oxygen evolution, while PS II function in the single mutant S88F appeared to be close to normal. Because of the more pronounced phenotype of the S79F/S88F strain as compared to the single mutants, both Ser residues appear to affect stable assembly and function of the PS II complex. The mechanism by which the S79F mutant loses photoautotrophic growth remains to be established. However, these results show the potential of targeted random mutagenesis to identify functionally important residues in selected regions of proteins.
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  • 100
    ISSN: 1573-5028
    Keywords: cDNA cloning ; gene expression ; glutamine synthetase ; grapevine ; nitrogen ; Vitis vinifera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Grapevine (Vitis vinifera L.) glutamine synthetase (GS) was analysed into two distinct classes of isoforms; one of them was present in both leaf and root tissues while the other one showed leaf specificity. Western blot analysis revealed that grapevine GS consists of three types of polypeptides of distinct size and differential tissue specificity. Two structurally distinct cDNA clones, pGS1;1 and pGS1;2, encoding grapevine GS were isolated from a cell suspension library and characterized. Both clones contained open reading frames encoding for polypeptides of 356 amino acids with a predicted molecular mass of about 39 kDa. Although the coding sequences of pGS1;1 and pGS1;2 were 84% similar, their 5′-and 3′-untranslated sequences showed only 40% similarity. The coding sequences of the two clones and the derived amino acid sequences showed higher homology to cytosolic than to chloroplastic GSs of other higher plants indicating that the cDNAs isolated encode for cytosolic isoforms of grapevine GS. Southern blot analysis suggested the existence of more than two GS genes in the grapevine genome. In northern blots both clones were hybridized to mRNAs of about 1.4 kb that are differentially expressed in the various tissues. Supply of nitrate or ammonium in the cell suspension culture medium, as a sole nitrogen source, resulted in differential response of the pGS1;1-and pGS1;2-related genes.
    Type of Medium: Electronic Resource
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