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  • 1
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    MDPI
    Publication Date: 2023-11-22
    Description: Climate change and urban development threaten health, undermine coping and deepen existing social and environmental inequities. A changing global environment requires transformative social responses: new partnerships, deep engagement with local communities, and innovations to strengthen individual and collective assets. The chapters of this proposed edited volume will be contributed mainly by established and emerging scholars from individuals, representing social work, sociology, development studies, law, government, social anthropology, urbanism, The chapters of this proposed edited volume will be contributed mainly by established and emerging scholars from individuals representing social work, sociology, development studies, public policy, and other social sciences This book is to be used for academics, policy makers, social work students, lecturers and other stakeholders to promote advocacy for vulnerable client groups affected by Climate Change. It gives some measure of hope and make the invisible visible to change.
    Keywords: Social Science ; Sociology ; Social Science ; Urban ; bic Book Industry Communication::J Society & social sciences::JF Society & culture: general ; bic Book Industry Communication::J Society & social sciences::JH Sociology & anthropology::JHB Sociology ; bic Book Industry Communication::J Society & social sciences::JF Society & culture: general::JFS Social groups::JFSG Urban communities
    Language: English
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  • 2
    Publication Date: 2021-06-25
    Description: Seeps are the expression of the migration of hydrocarbons from subsurface accumulations to the surface in sedimentary basins. They may represent an important indication of the presence of petroleum (gas and oil) reservoirs and faults, and are a natural source of greenhouse gas (methane) and atmospheric pollutants (ethane, propane) to the atmosphere. Romania is one of the countries with the largest number of seeps in the world, due to the high petroleum potential and active tectonics. Based on a review of the available literature, and on the field surveys performed by the authors during the last 17 years, we report the first comprehensive GIS-based inventory of 470 seeps in Romania (HYSED-RO), including gas seeps (10.4% of the total), oil seeps (11.7%), mud volcanoes (50.4%), gas-rich springs (12.6%), asphalt (solid) seeps (4.3%), unclassified manifestations (4.0%), and uncertain seeps (6.6%). Seeps are typically located in correspondence with major faults and vertical and fractured stratigraphic contacts associated to petroleum reservoirs (anticlines) in low heat flow areas, and their gas-geochemistry reflects that of the subsurface reservoirs. The largest and most active seeps occur in the Carpathian Foredeep, where they release thermogenic gas, and subordinately in the Transylvanian Basin, where gas is mainly microbial. HYSED-RO may represent a key reference for baseline characterization prior to subsurface petroleum extraction, for environmental studies, and atmospheric greenhouse gas emission estimates in Romania.
    Description: Published
    Description: 39
    Description: 6A. Geochimica per l'ambiente
    Description: JCR Journal
    Keywords: hydrocarbon seeps ; methane ; mud volcanoes ; petroleum systems ; seep database ; 03.04. Chemical and biological
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 3
    Publication Date: 2019-12-03
    Description: Phytoplankton in the ocean are extremely diverse. The abundance of various intracellular pigments are often used to study phytoplankton physiology and ecology, and identify and quantify different phytoplankton groups. In this study, phytoplankton absorption spectra (aph(λ)) derived from underway flow-through AC-S measurements in the Fram Strait are combined with phytoplankton pigment measurements analyzed by high-performance liquid chromatography (HPLC) to evaluate the retrieval of various pigment concentrations at high spatial resolution. The performances of two approaches, Gaussian decomposition and the matrix inversion technique are investigated and compared. Our study is the first to apply the matrix inversion technique to underway spectrophotometry data. We find that Gaussian decomposition provides good estimates (median absolute percentage error, MPE 21–34%) of total chlorophyll-a (TChl-a), total chlorophyll-b (TChl-b), the combination of chlorophyll-c1 and -c2 (Chl-c1/2), photoprotective (PPC) and photosynthetic carotenoids (PSC). This method outperformed one of the matrix inversion algorithms, i.e., singular value decomposition combined with non-negative least squares (SVD-NNLS), in retrieving TChl-b, Chl-c1/2, PSC, and PPC. However, SVD-NNLS enables robust retrievals of specific carotenoids (MPE 37–65%), i.e., fucoxanthin, diadinoxanthin and 19′-hexanoyloxyfucoxanthin, which is currently not accomplished by Gaussian decomposition. More robust predictions are obtained using the Gaussian decomposition method when the observed aph(λ) is normalized by the package effect index at 675 nm. The latter is determined as a function of “packaged” aph(675) and TChl-a concentration, which shows potential for improving pigment retrieval accuracy by the combined use of aph(λ) and TChl-a concentration data. To generate robust estimation statistics for the matrix inversion technique, we combine leave-one-out cross-validation with data perturbations. We find that both approaches provide useful information on pigment distributions, and hence, phytoplankton community composition indicators, at a spatial resolution much finer than that can be achieved with discrete samples.
    Repository Name: EPIC Alfred Wegener Institut
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  • 4
    Publication Date: 2018-08-28
    Description: Exposed stones in sandy sublittoral environments are hotspots for marine biodiversity, especially for benthic communities. The detection of single stones is principally possible using sidescan-sonar (SSS) backscatter data. The data resolution has to be high to visualize the acoustic shadows of the stones. Otherwise, stony substrates will not be differentiable from other high backscatter substrates (e.g., gravel). Acquiring adequate sonar data and identifying stones in backscatter images is time consuming because it usually requires visual-manual procedures. To develop a more efficient identification and demarcation procedure of stone fields, sidescan sonar and parametric echo sound data were recorded within the marine protected area of “Sylt Outer Reef” (German Bight, North Sea). The investigated area (~5.900 km2) is characterized by dispersed heterogeneous moraine and marine deposits. Data from parametric sediment echo sounder indicate hyperbolas at the sediment surface in stony areas, which can easily be exported. By combining simultaneous recorded low backscatter data and parametric single beam data, stony grounds were demarcated faster, less complex and reproducible from gravelly substrates indicating similar high backscatter in the SSS data.
    Repository Name: EPIC Alfred Wegener Institut
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  • 5
    Publication Date: 2018-08-28
    Description: Backscatter mosaics based on a multi-frequency multibeam echosounder survey in the continental shelf setting of the North Sea were compared. The uncalibrated backscatter data were recorded with frequencies of 200, 400 and 600 kHz. The results showed that the seafloor appears mostly featureless in acoustic backscatter mosaics derived from 600 kHz data. The same area surveyed with 200 kHz reveals numerous backscatter anomalies with diameters of 10–70 m deviating between −2 dB and +4 dB from the background sediment. Backscatter anomalies were further subdivided based on their frequency-specific texture and were attributed to bioturbation within the sediment and the presence of polychaetes on the seafloor. While low frequencies show the highest overall contrast between different seafloor types, a consideration of all frequencies permits an improved interpretation of subtle seafloor features.
    Repository Name: EPIC Alfred Wegener Institut
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  • 6
    Publication Date: 2018-09-10
    Description: The study presented here is focused on the assessment of surface elevations derived from CryoSat-2 SARIn level 1b data over the Austfonna ice cap, Svalbard, in 2016. The processing chain that must be applied to the CryoSat-2 waveforms to derive heights is non-trivial, and consists of multiple steps, all requiring subjective choices of methods such as the choice of retracker, geo-relocation, and outlier rejection. Here, we compare six CryoSat-2 level-2 type data sets of surface elevations derived using different SARIn processing chains. These data sets are validated against surface elevation data collected from an airborne laser scanner, during a dedicated CryoSat validation experiment field campaign carried out in April 2016. The flight pattern of the airborne campaign was designed so that elevations were measured in a grid pattern rather than along single lines, as has previously been the standard procedure. The flight grid pattern was chosen to optimize the comparison with the CryoSat-2 SARIn elevation data, the location of which can deviate from nadir by several kilometers due to topography within the satellite footprint. The processing chains behind the six data sets include different outlier/error rejection approaches, and do not produce the same number of data points in our region of interest. To make a consistent analysis, we provide statistics from the validation of both the full data sets from each processing chain, and on only those data that all the six data sets provide a geo-located elevation estimate for. We find that the CryoSat-2 data sets that agree best with the validation data are those derived from dedicated land ice processing schemes. This study may serve as a benchmark for future CryoSat-2 retracker developments, and the evaluation software and data set are made publicly available.
    Repository Name: EPIC Alfred Wegener Institut
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  • 7
    Publication Date: 2019-08-23
    Description: The timing of snowmelt is an important turning point in the seasonal cycle of small Arctic catchments. The TerraSAR-X (TSX) satellite mission is a synthetic aperture radar system (SAR) with high potential to measure the high spatiotemporal variability of snow cover extent (SCE) and fractional snow cover (FSC) on the small catchment scale. We investigate the performance of multi-polarized and multi-pass TSX X-Band SAR data in monitoring SCE and FSC in small Arctic tundra catchments of Qikiqtaruk (Herschel Island) off the Yukon Coast in the Western Canadian Arctic. We applied a threshold based segmentation on ratio images between TSX images with wet snow and a dry snow reference, and tested the performance of two different thresholds. We quantitatively compared TSX- and Landsat 8-derived SCE maps using confusion matrices and analyzed the spatiotemporal dynamics of snowmelt from 2015 to 2017 using TSX, Landsat 8 and in situ time lapse data. Our data showed that the quality of SCE maps from TSX X-Band data is strongly influenced by polarization and to a lesser degree by incidence angle. VH polarized TSX data performed best in deriving SCE when compared to Landsat 8. TSX derived SCE maps from VH polarization detected late lying snow patches that were not detected by Landsat 8. Results of a local assessment of TSX FSC against the in situ data showed that TSX FSC accurately captured the temporal dynamics of different snow melt regimes that were related to topographic characteristics of the studied catchments. Both in situ and TSX FSC showed a longer snowmelt period in a catchment with higher contributions of steep valleys and a shorter snowmelt period in a catchment with higher contributions of upland terrain. Landsat 8 had fundamental data gaps during the snowmelt period in all 3 years due to cloud cover. The results also revealed that by choosing a positive threshold of 1 dB, detection of ice layers due to diurnal temperature variations resulted in a more accurate estimation of snow cover than a negative threshold that detects wet snow alone. We find that TSX X-Band data in VH polarization performs at a comparable quality to Landsat 8 in deriving SCE maps when a positive threshold is used. We conclude that TSX data polarization can be used to accurately monitor snowmelt events at high temporal and spatial resolution, overcoming limitations of Landsat 8, which due to cloud related data gaps generally only indicated the onset and end of snowmelt.
    Repository Name: EPIC Alfred Wegener Institut
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  • 8
    Publication Date: 2018-10-15
    Description: Yedoma—extremely ice-rich permafrost with massive ice wedges formed during the Late Pleistocene—is vulnerable to thawing and degradation under climate warming. Thawing of ice-rich Yedoma results in lowering of surface elevations. Quantitative knowledge about surface elevation changes helps us to understand the freeze-thaw processes of the active layer and the potential degradation of Yedoma deposits. In this study, we use C-band Sentinel-1 InSAR measurements to map the elevation changes over ice-rich Yedoma uplands on Sobo-Sise Island, Lena Delta with frequent revisit observations (as short as six or 12 days). We observe significant seasonal thaw subsidence during summer months and heterogeneous inter-annual elevation changes from 2016–17. We also observe interesting patterns of stronger seasonal thaw subsidence on elevated flat Yedoma uplands by comparing to the surrounding Yedoma slopes. Inter-annual analyses from 2016–17 suggest that our observed positive surface elevation changes are likely caused by the delayed progression of the thaw season in 2017, associated with mean annual air temperature fluctuations.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev , info:eu-repo/semantics/article
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  • 9
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    American Chemical Society
    In:  EPIC3Environmental Science & Technology, American Chemical Society, 52(22), pp. 13279-13288
    Publication Date: 2019-03-13
    Repository Name: EPIC Alfred Wegener Institut
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  • 10
    Publication Date: 2021-02-16
    Description: Arctic and subarctic regions are sensitive to climate change and, reversely, provide dramatic feedbacks to the global climate. With a focus on discovering paleoclimate and paleoceanographic evolution in the Arctic and Northwest Pacific Oceans during the last 20,000 years, we proposed this German–Sino cooperation program according to the announcement “Federal Ministry of Education and Research (BMBF) of the Federal Republic of Germany for a German–Sino cooperation program in the marine and polar research”. Our proposed program integrates the advantages of the Arctic and Subarctic marine sediment studies in AWI (Alfred Wegener Institute) and FIO (First Institute of Oceanography). For the first time, the collection of sediment cores can cover all climatological key regions in the Arctic and Northwest Pacific Oceans. Furthermore, the climate modeling work at AWI enables a “Data-Model Syntheses”, which are crucial for exploring the underlying mechanisms of observed changes in proxy records.
    Repository Name: EPIC Alfred Wegener Institut
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  • 11
    Publication Date: 2019-05-28
    Description: An intercomparison of radiance and irradiance ocean color radiometers (the second laboratory comparison exercise—LCE-2) was organized within the frame of the European Space Agency funded project Fiducial Reference Measurements for Satellite Ocean Color (FRM4SOC) May 8–13, 2017 at Tartu Observatory, Estonia. LCE-2 consisted of three sub-tasks: (1) SI-traceable radiometric calibration of all the participating radiance and irradiance radiometers at the Tartu Observatory just before the comparisons; (2) indoor, laboratory intercomparison using stable radiance and irradiance sources in a controlled environment; (3) outdoor, field intercomparison of natural radiation sources over a natural water surface. The aim of the experiment was to provide a link in the chain of traceability from field measurements of water reflectance to the uniform SI-traceable calibration, and after calibration to verify whether di�erent instruments measuring the same object provide results consistent within the expected uncertainty limits. This paper describes the third phase of LCE-2: The results of the field experiment. The calibration of radiometers and laboratory comparison experiment are presented in a related paper of the same journal issue. Compared to the laboratory comparison, the field intercomparison has demonstrated substantially larger variability between freshly calibrated sensors, because the targets and environmental conditions during radiometric calibration were di�erent, both spectrally and spatially. Major di�erences were found for radiance sensors measuring a sunlit water target at viewing zenith angle of 139� because of the di�erent fields of view. Major di�erences were found for irradiance sensors because of imperfect cosine response of di�users. Variability between individual radiometers did depend significantly also on the type of the sensor and on the specific measurement target. Uniform SI traceable radiometric calibration ensuring fairly good consistency for indoor, laboratory measurements is insu�cient for outdoor, field measurements, mainly due to the di�erent angular variability of illumination. More stringent specifications and individual testing of radiometers for all relevant systematic e�ects (temperature, nonlinearity, spectral stray light, etc.) are needed to reduce biases between instruments and better quantify measurement uncertainties.
    Repository Name: EPIC Alfred Wegener Institut
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  • 12
    Publication Date: 2019-05-28
    Description: An intercomparison of radiance and irradiance ocean color radiometers (The Second Laboratory Comparison Exercise—LCE-2) was organized within the frame of the European Space Agency funded project Fiducial Reference Measurements for Satellite Ocean Color (FRM4SOC) May 8–13, 2017 at Tartu Observatory, Estonia. LCE-2 consisted of three sub-tasks: 1) SI-traceable radiometric calibration of all the participating radiance and irradiance radiometers at the Tartu Observatory just before the comparisons; 2) Indoor intercomparison using stable radiance and irradiance sources in controlled environment; and 3) Outdoor intercomparison of natural radiation sources over terrestrial water surface. The aim of the experiment was to provide one link in the chain of traceability from field measurements of water reflectance to the uniform SI-traceable calibration, and after calibration to verify whether di�erent instruments measuring the same object provide results consistent within the expected uncertainty limits. This paper describes the activities and results of the first two phases of LCE-2: the SI-traceable radiometric calibration and indoor intercomparison, the results of outdoor experiment are presented in a related paper of the same journal issue. The indoor experiment of the LCE-2 has proven that uniform calibration just before the use of radiometers is highly e�ective. Distinct radiometers from di�erent manufacturers operated by di�erent scientists can yield quite close radiance and irradiance results (standard deviation s 〈 1%) under defined conditions. This holds when measuring stable lamp-based targets under stationary laboratory conditions with all the radiometers uniformly calibrated against the same standards just prior to the experiment. In addition, some unification of measurement and data processing must be settled. Uncertaint of radiance and irradiance measurement under these conditions largely consists of the sensor’s calibration uncertainty and of the spread of results obtained by individual sensors measuring the same object.
    Repository Name: EPIC Alfred Wegener Institut
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  • 13
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    MDPI
    In:  EPIC3Remote Sensing, MDPI, 11(9), pp. 1-18, ISSN: 2072-4292
    Publication Date: 2019-05-20
    Description: Real-time quality-controlled surface current data derived from X-Band marine radar (MR) measurements were evaluated to estimate their operational reliability. The presented data were acquired by the standard commercial o�-the-shelf MR-based sigma s6 WaMoS® II (WaMoS® II) deployed onboard the German Research vessel Polarstern. The measurement reliability is specified by an IQ value obtained by the WaMoS® II real-time quality control (rtQC). Data which pass the rtQC without objection are assumed to be reliable. For these data sets accuracy and correlation with corresponding vessel-mounted acoustic Doppler current profiler (ADCP) measurements are determined. To reduce potential misinterpretation due to short-term oceanic variability/turbulences, the evaluation of the WaMoS® II accuracy was carried out based on sliding means over 20 min of the reliable data only. The associated standard deviation �WaMoS = 0.02 m/s of the meanWaMoS® II measurements reflect a high precision of the measurement and the successful rtQC during di�erent wave, current and weather conditions. The direct comparison of 7272 WaMoS® II/ADCP northward and eastward velocity data pairs yield a correlation of r � 0.94, with jbiasDj � 0.06 m/s and �S = 0.05 m/s. This confirms that the MR-based surface current measurements are accurate and reliable.
    Repository Name: EPIC Alfred Wegener Institut
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  • 14
    Publication Date: 2019-09-01
    Description: Coring sediments in subglacial aquatic environments offers unique opportunities for research on paleo-environments and paleo-climates because it can provide data from periods even earlier than ice cores, as well as the overlying ice histories, interactions between ice and the water system, life forms in extreme habitats, sedimentology, and stratigraphy. However, retrieving sediment cores from a subglacial environment faces more difficulties than sediment coring in oceans and lakes, resulting in low yields from the most current subglacial sediment coring methods. The coring tools should pass through a hot water-drilled access borehole, then the water column, to reach the sediment layers. The access boreholes are size-limited by the hot water drilling tools and techniques. These holes are drilled through ice up to 3000–4000 m thick, with diameters ranging from 10–60 cm, and with a refreezing closure rate of up to 6 mm/h after being drilled. Several purpose-built streamline corers have been developed to pass through access boreholes and collect the sediment core. The main coring objectives are as follows: (i) To obtain undisturbed water–sediment cores, either singly or as multi-cores and (ii) to obtain long cores with minimal stratigraphic deformation. Subglacial sediment coring methods use similar tools to those used in lake and ocean coring. These methods include the following: Gravity coring, push coring, piston coring, hammer or percussion coring, vibrocoring, and composite methods. Several core length records have been attained by different coring methods, including a 290 cm percussion core from the sub-ice-shelf seafloor, a 400 cm piston core from the sub-ice-stream, and a 170 cm gravity core from a subglacial lake. There are also several undisturbed water–sediment cores that have been obtained by gravity corers or hammer corers. Most current coring tools are deployed by winch and cable facilities on the ice surface. There are three main limitations for obtaining long sediment cores which determines coring tool development, as follows: Hot-water borehole radial size restriction, the sedimentary structure, and the coring techniques. In this paper, we provide a general view on current developments in coring tools, including the working principles, corer characteristics, operational methods, coring site locations, field conditions, coring results, and possible technical improvements. Future prospects in corer design and development are also discussed.
    Repository Name: EPIC Alfred Wegener Institut
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  • 15
    Publication Date: 2019-10-21
    Description: Air temperatures in the Arctic have increased substantially over the last decades, which has extensively altered the properties of the land surface. Capturing the state and dynamics of Land Surface Temperatures (LSTs) at high spatial detail is of high interest as LST is dependent on a variety of surficial properties and characterizes the land–atmosphere exchange of energy. Accordingly, this study analyses the influence of different physical surface properties on the long-term mean of the summer LST in the Arctic Mackenzie Delta Region (MDR) using Landsat 30 m-resolution imagery between 1985 and 2018 by taking advantage of the cloud computing capabilities of the Google Earth Engine. Multispectral indices, including the Normalized Difference Vegetation Index (NDVI), Normalized Difference Water Index (NDWI) and Tasseled Cap greenness (TCG), brightness (TCB), and wetness (TCW) as well as topographic features derived from the TanDEM-X digital elevation model are used in correlation and multiple linear regression analyses to reveal their influence on the LST. Furthermore, surface alteration trends of the LST, NDVI, and NDWI are revealed using the Theil-Sen (T-S) regression method. The results indicate that the mean summer LST appears to be mostly influenced by the topographic exposition as well as the prevalent moisture regime where higher evapotranspiration rates increase the latent heat flux and cause a cooling of the surface, as the variance is best explained by the TCW and northness of the terrain. However, fairly diverse model outcomes for different regions of the MDR (R2 from 0.31 to 0.74 and RMSE from 0.51 °C to 1.73 °C) highlight the heterogeneity of the landscape in terms of influential factors and suggests accounting for a broad spectrum of different factors when modeling mean LSTs. The T-S analysis revealed large-scale wetting and greening trends with a mean decadal increase of the NDVI/NDWI of approximately +0.03 between 1985 and 2018, which was mostly accompanied by a cooling of the land surface given the inverse relationship between mean LSTs and vegetation and moisture conditions. Disturbance through wildfires intensifies the surface alterations locally and lead to significantly cooler LSTs in the long-term compared to the undisturbed surroundings.
    Repository Name: EPIC Alfred Wegener Institut
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  • 16
    Publication Date: 2021-07-23
    Description: In this work, laboratory tests with live bivalves as well as the conceptual design of additively manufactured surrogate models are presented. The overall task of this work is to develop a surrogate best fitting to the live mussels tested in accordance to the identified surface descriptor, i.e., the Abbott–Firestone Curve, and to the hydrodynamic behaviour by means of drag and inertia coefficients. To date, very few investigations have focused on loads from currents as well as waves. Therefore, tests with a towing carriage were carried out in a wave flume. A custom-made rack using mounting clamps was built to facilitate carriage-run tests with minimal delays. Blue mussels (Mytilus edulis) extracted from a site in Germany, which were kept in aerated seawater to ensure their survival for the test duration, were used. A set of preliminary results showed drag and inertia coefficients CD and CM ranging from 1.16–3.03 and 0.25 to 1.25. To derive geometrical models of the mussel dropper lines, 3-D point clouds were prepared by means of 3-D laser scanning to obtain a realistic surface model. Centered on the 3-D point cloud, a suitable descriptor for the mass distribution over the surface was identified and three 3-D printed surrogates of the blue mussel were developed for further testing. These were evaluated regarding their fit to the original 3-D point cloud of the live blue mussels via the chosen surface descriptor.
    Repository Name: EPIC Alfred Wegener Institut
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  • 17
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    American Chemical Society
    In:  EPIC3Environmental Science & Technology, American Chemical Society, 53, pp. 8747-8756, ISSN: 1520-5851
    Publication Date: 2020-06-04
    Description: Recent studies pointed to a high ice nucleating activity (INA) in the Arctic sea surface microlayer (SML). However, related chemical information is still sparse. In the present study, INA and free glucose concentrations were quantified in Arctic SML and bulk water samples from the marginal ice zone, the ice-free ocean, melt ponds, and open waters within the ice pack. T50 (defining INA) ranged from −17.4 to −26.8 °C. Glucose concentrations varied from 0.6 to 51 μg/L with highest values in the SML from the marginal ice zone and melt ponds (median 16.3 and 13.5 μg/L) and lower values in the SML from the ice pack and the ice-free ocean (median 3.9 and 4.0 μg/L). Enrichment factors between the SML and the bulk ranged from 0.4 to 17. A positive correlation was observed between free glucose concentration and INA in Arctic water samples (T50(°C) = (−25.6 ± 0.6) + (0.15 ± 0.04)·Glucose(μg/L), RP = 0.66, n = 74). Clustering water samples based on phytoplankton pigment composition resulted in robust but different correlations within the four clusters (RP between 0.67 and 0.96), indicating a strong link to phytoplankton-related processes. Since glucose did not show significant INA itself, free glucose may serve as a potential tracer for INA in Arctic water samples.
    Repository Name: EPIC Alfred Wegener Institut
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  • 18
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    American Chemical Society
    In:  EPIC3Environmental Science and Technology, American Chemical Society, 50(13), pp. 7066-7073, ISSN: 0013936X
    Publication Date: 2016-10-12
    Description: Atmospheric nuclear weapons testing (NWT) resulted in the injection of plutonium (Pu) into the atmosphere and subsequent global deposition. We present a new method for continuous semiquantitative measurement of 239Pu in ice cores, which was used to develop annual records of fallout from NWT in ten ice cores from Greenland and Antarctica. The 239Pu was measured directly using an inductively coupled plasma-sector field mass spectrometer, thereby reducing analysis time and increasing depth-resolution with respect to previous methods. To validate this method, we compared our one year averaged results to published 239Pu records and other records of NWT. The 239Pu profiles from the Arctic ice cores reflected global trends in NWT and were in agreement with discrete Pu profiles from lower latitude ice cores. The 239Pu measurements in the Antarctic ice cores tracked low latitude NWT, consistent with previously published discrete records from Antarctica. Advantages of the continuous 239Pu measurement method are (1) reduced sample preparation and analysis time; (2) no requirement for additional ice samples for NWT fallout determinations; (3) measurements are exactly coregistered with all other chemical, elemental, isotopic, and gas measurements from the continuous analytical system; and (4) the long half-life means the 239Pu record is stable through time.
    Repository Name: EPIC Alfred Wegener Institut
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  • 19
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    MDPI
    In:  EPIC3Remote Sensing, MDPI, 8(871), pp. 1-21, ISSN: ISSN 2072-4292
    Publication Date: 2016-11-08
    Description: Studying phytoplankton functional types (PFTs) from space is possible due to recent advances in remote sensing. Though a variety of products are available, the limited number of wavelengths available compared to the number of model parameters needed to be retrieved is still a major problem in using ocean-color data for PFT retrievals. Here, we investigated which band placement could improve retrievals of three particular PFTs (diatoms, coccolithophores and cyanobacteria). In addition to analyzing dominant spectral features in the absorption spectra of the target PFTs, two previously-developed methods using measured spectra were applied to simulated data. Such a synthetic dataset allowed for significantly increasing the number of scenarios and enabled a full control over parameters causing spectral changes. We evaluated the chosen band placement by applying an adapted ocean reflectance inversion, as utilized in the generalized inherent optical properties (GIOP) retrieval. Results show that the optimal band settings depend on the method applied to determine the bands placement, as well as on the internal variability of the dataset investigated. Therefore, continuous hyperspectral instruments would be most beneficial for discriminating multiple PFTs, though a small improvement in spectral sampling and resolution does not significantly modify the results. Bands, which could be added to future instruments (e.g., Ocean and Land Colour Instrument (OLCI) instrument on the upcoming Sentinel-3B,-3C,-3D, etc., and further satellites) in order to enhance PFT retrieval capabilities, were also determined.
    Repository Name: EPIC Alfred Wegener Institut
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  • 20
    Publication Date: 2016-05-20
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  • 21
    Publication Date: 2020-06-14
    Description: We analyzed chlorophyll-a and Colored Dissolved Organic Matter (CDOM) dynamics from field measurements and assessed the potential of multispectral satellite data for retrieving water-quality parameters in three small surface reservoirs in the Brazilian semiarid region. More specifically, this work is comprised of: (i) analysis of Chl-a and trophic dynamics; (ii) characterization of CDOM; (iii) estimation of Chl-a and CDOM from OLI/Landsat-8 and RapidEye imagery. The monitoring lasted 20 months within a multi-year drought, which contributed to water-quality deterioration. Chl-a and trophic state analysis showed a highly eutrophic status for the perennial reservoir during the entire study period, while the non-perennial reservoirs ranged from oligotrophic to eutrophic, with changes associated with the first events of the rainy season. CDOM characterization suggests that the perennial reservoir is mostly influenced by autochthonous sources, while allochthonous sources dominate the non-perennial ones. Spectral-group classification assigned the perennial reservoir as a CDOM-moderate and highly eutrophic reservoir, whereas the non-perennial ones were assigned as CDOM-rich and oligotrophic-dystrophic reservoirs. The remote sensing initiative was partially successful: the Chl-a was best modelled using RapidEye for the perennial one; whereas CDOM performed best with Landsat-8 for non-perennial reservoirs. This investigation showed potential for retrieving water quality parameters in dry areas with small reservoirs
    Repository Name: EPIC Alfred Wegener Institut
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  • 22
    Publication Date: 2018-03-02
    Description: In this study, we analyze interactions in lake and lake catchment systems of a continuous permafrost area. We assessed colored dissolved organic matter (CDOM) absorption at 440 nm (a(440)CDOM) and absorption slope (S300–500) in lakes using field sampling and optical remote sensing data for an area of 350 km2 in Central Yamal, Siberia. Applying a CDOM algorithm (ratio of green and red band reflectance) for two high spatial resolution multispectral GeoEye-1 and Worldview-2 satellite images, we were able to extrapolate the a(λ)CDOM data from 18 lakes sampled in the field to 356 lakes in the study area (model R2 = 0.79). Values of a(440)CDOM in 356 lakes varied from 0.48 to 8.35 m−1 with a median of 1.43 m−1. This a(λ)CDOM dataset was used to relate lake CDOM to 17 lake and lake catchment parameters derived from optical and radar remote sensing data and from digital elevation model analysis in order to establish the parameters controlling CDOM in lakes on the Yamal Peninsula. Regression tree model and boosted regression tree analysis showed that the activity of cryogenic processes (thermocirques) in the lake shores and lake water level were the two most important controls, explaining 48.4% and 28.4% of lake CDOM, respectively (R2 = 0.61). Activation of thermocirques led to a large input of terrestrial organic matter and sediments from catchments and thawed permafrost to lakes (n = 15, mean a(440)CDOM = 5.3 m−1). Large lakes on the floodplain with a connection to Mordy-Yakha River received more CDOM (n = 7, mean a(440)CDOM = 3.8 m−1) compared to lakes located on higher terraces.
    Repository Name: EPIC Alfred Wegener Institut
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  • 23
    Publication Date: 2018-03-12
    Description: We studied two issues to be considered in the calculation of parameters characterizing sea ice deformation: the effect of uncertainties in an automatically retrieved sea ice drift field, and the influence of the type of drift vector grid. Sea ice deformation changes the local ice mass balance and the interaction between atmosphere, ice, and ocean, and constitutes a hazard to marine traffic and operations. Due to numerical effects, the results of deformation retrievals may predict, e.g., openings and closings of the ice cover that do not exist in reality. We focus specifically on fields of ice drift obtained from synthetic aperture radar (SAR) imagery and analyze the Propagated Drift Retrieval Error (PDRE) and the Boundary Definition Error (BDE). From the theory of error propagation, the PDRE for the calculated deformation parameters can be estimated. To quantify the BDE, we devise five different grid types and compare theoretical expectation and numerical results for different deformation parameters assuming three scenarios: pure divergence, pure shear, and a mixture of both. Our findings for both sources of error help to set up optimal deformation retrieval schemes and are also useful for other applications working with vector fields and scalar parameters derived therefrom.
    Repository Name: EPIC Alfred Wegener Institut
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  • 24
    facet.materialart.
    Unknown
    American Chemical Society
    In:  EPIC3Crystal Growth & Design, American Chemical Society, 18, pp. 2563-2571
    Publication Date: 2018-04-23
    Description: The morphology and growth kinetics of ice single crystals in aqueous solutions of type III antifreeze protein (AFP-III) have been studied in detail over a range of AFP-III concentrations and supercoolings. In pure water, the shape of ice crystals changes from the circular disklike to planar dendritic with increasing supercooling. In AFP-III solutions, ice crystals in the form of faceted plates, irregular dendrites with polygonized tips, and needles appear with increasing supercooling and AFP-III concentration. The growth rate of ice crystals in the crystallographic a direction is 2 orders of magnitude higher than that in the c direction. AFP-III molecules cause the stoppage of the growth of the prismatic and basal faces at low supercoolings. When supercooling exceeds the critical value, AFP-III favors the acceleration of the growth in both a and c directions. The observed behavior of AFP-III is explained in terms of the Cabrera-Vermilyea pinning model and the specificity of the dissipation of latent heat from the growing crystals with different shapes.
    Repository Name: EPIC Alfred Wegener Institut
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  • 25
    Publication Date: 2017-04-04
    Description: Abstract: The conservation of architectural heritage usually requires a multidisciplinary approach involving a variety of specialist expertise and techniques. Nevertheless, destructive techniques should be avoided, wherever possible, in order to preserve the integrity of the historical buildings, therefore the development of non-destructive and non-contact techniques is extremely important. In this framework, a methodology for combining the terrestrial laser scanning and the infrared thermal images is proposed, in order to obtain a reconnaissance of the conservation state of a historical building. The proposed case study is represented by St. Augustine Monumental Compound, located in the historical centre of the town of Cosenza (Calabria, South Italy). Adopting the proposed methodology, the paper illustrates the main results obtained for the building test overlaying and comparing the collected data with both techniques, in order to outline the capabilities both to detect the anomalies and to improve the knowledge on health state of the masonry building. The 3D model, also, allows to provide a reference model, laying the groundwork for implementation of a monitoring multisensor system based on the use of non-destructive techniques.
    Description: The presented work has been carried out in the PON Monitoraggio in Area Sismica di SIstemi MOnumentali (MASSIMO) project framework, managed by the Italian National Institute of Geophysics and Volcanology (INGV) and funded by the Italian Ministry of Education, University and Research
    Description: Published
    Description: 194-213
    Description: 6A. Monitoraggio ambientale, sicurezza e territorio
    Description: JCR Journal
    Description: open
    Keywords: terrestrial laser scanner ; IR thermography ; historical buildings conservation ; preventive diagnosis ; 05. General::05.04. Instrumentation and techniques of general interest::05.04.99. General or miscellaneous
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 26
    Publication Date: 2017-04-04
    Description: The three volcanoes that are the object of this paper show different types of activity that are representative of the large variety of volcanism present in the Central Mediterranean area. Etna and Stromboli are sub-aerial volcanoes, with significant part of their structure under the sea, while the Marsili Seamount is submerged, and its activity is still open to debate. The study of these volcanoes can benefit from multi-parametric observations from the seafloor. Each volcano was studied with a different kind of observation system. Stromboli seismic recordings are acquired by means of a single Ocean Bottom Seismometer (OBS). From these data, it was possible to identify two different magma chambers at different depths. At Marsili Seamount, gravimetric and seismic signals are recorded by a battery-powered multi-disciplinary observatory (GEOSTAR). Gravimetric variations and seismic Short Duration Events (SDE) confirm the presence of hydrothermal activity. At the Etna observation site, seismic signals, water pressure, magnetic field and acoustic echo intensity are acquired in real-time thanks to a cabled multi-disciplinary observatory (NEMO-SN1 ). This observatory is one of the operative nodes of the European Multidisciplinary Seafloor and water-column Observatory (EMSO; www.emso-eu.org) research infrastructure. Through a multidisciplinary approach, we speculate about deep Etna sources and follow some significant events, such as volcanic ash diffusion in the seawater.
    Description: Published
    Description: 298
    Description: 3A. Ambiente Marino
    Description: JCR Journal
    Description: restricted
    Keywords: EMSO ; volcanic ash clouds ; seafloor observatories ; stand-alone monitoring systems ; volcano seismology ; 03. Hydrosphere::03.04. Chemical and biological::03.04.08. Instruments and techniques ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring ; 04. Solid Earth::04.08. Volcanology::04.08.07. Instruments and techniques ; 05. General::05.02. Data dissemination::05.02.03. Volcanic eruptions
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 27
    Publication Date: 2017-04-04
    Description: In recent years, an increasing number of surveys have definitively confirmed the seasonal presence of fin whales (Balaenoptera physalus) in highly productive regions of the Mediterranean Sea. Despite this, very little is yet known about the routes that the species seasonally follows within the Mediterranean basin and, particularly, in the Ionian area. The present study assesses for the first time fin whale acoustic presence offshore Eastern Sicily (Ionian Sea), throughout the processing of about 10 months of continuous acoustic monitoring. The recording of fin whale vocalizations was made possible by the cabled deep-sea multidisciplinary observatory, “NEMO-SN1”, deployed 25 km off the Catania harbor at a depth of about 2,100 meters. NEMO-SN1 is an operational node of the European Multidisciplinary Seafloor and water-column Observatory (EMSO) Research Infrastructure. The observatory was equipped with a low-frequency hydrophone (bandwidth: 0.05 Hz–1 kHz, sampling rate: 2 kHz) which continuously acquired data from July 2012 to May 2013. About 7,200 hours of acoustic data were analyzed by means of spectrogram display. Calls with the typical structure and patterns associated to the Mediterranean fin whale population were identified and monitored in the area for the first time. Furthermore, a background noise analysis within the fin whale communication frequency band (17.9–22.5 Hz) was conducted to investigate possible detection-masking effects. The study confirms the hypothesis that fin whales are present in the Ionian Sea throughout all seasons, with peaks in call detection rate during spring and summer months. The analysis also demonstrates that calls were more frequently detected in low background noise conditions. Further analysis will be performed to understand whether observed levels of noise limit the acoustic detection of the fin whales vocalizations, or whether the animals vocalize less in the presence of high background noise.
    Description: Published
    Description: e0141838
    Description: 3A. Ambiente Marino
    Description: JCR Journal
    Description: open
    Keywords: Whales ; Bioacoustics ; Background noise (acoustics) ; Acoustic signals ; Sperm whales ; Vocalization ; Acoustics ; Data acquisition ; 03. Hydrosphere::03.01. General::03.01.08. Instruments and techniques ; 03. Hydrosphere::03.02. Hydrology::03.02.04. Measurements and monitoring ; 03. Hydrosphere::03.02. Hydrology::03.02.07. Instruments and techniques ; 03. Hydrosphere::03.04. Chemical and biological::03.04.99. General or miscellaneous
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 28
    Publication Date: 2019-03-28
    Description: The understanding of the dynamical properties of skyrmion is a fundamental aspect for the realization of a competitive skyrmion based technology beyond CMOS. Most of the theoretical approaches are based on the approximation of a rigid skyrmion. However, thermal fluctuations can drive a continuous change of the skyrmion size via the excitation of thermal modes. Here, by taking advantage of the Hilbert-Huang transform, we demonstrate that at least two thermal modes can be excited which are non-stationary in time. In addition, one limit of the rigid skyrmion approximation is that this hypothesis does not allow for correctly describing the recent experimental evidence of skyrmion Hall angle dependence on the amplitude of the driving force, which is proportional to the injected current. In this work, we show that, in an ideal sample, the combined effect of field-like and damping-like torques on a breathing skyrmion can indeed give rise to such a current dependent skyrmion Hall angle. While here we design and control the breathing mode of the skyrmion, our results can be linked to the experiments by considering that the thermal fluctuations and/or disorder can excite the breathing mode. We also propose an experiment to validate our findings.
    Description: Published
    Description: 224418
    Description: 1A. Geomagnetismo e Paleomagnetismo
    Description: 2IT. Laboratori analitici e sperimentali
    Description: JCR Journal
    Keywords: Physics - Mesoscopic Systems and Quantum Hall Effect ; Physics - Mesoscopic Systems and Quantum Hall Effect
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 29
    Publication Date: 2019-06-17
    Description: Automatic detection of icebergs in satellite images is regarded a useful tool to provide information necessary for safety in Arctic shipping or operations over large ocean areas in near-real time. In this work, we investigated the feasibility of automatic iceberg detection in Sentinel-1 Extra Wide Swath (EWS) SAR images which follow the preferred image mode in operational ice charting. As test region, we selected the Barents Sea where the size of many icebergs is on the order of the spatial resolution of the EWS-mode. We tested a new approach for a detection scheme. It is based on a combination of a filter for enhancing the contrast between icebergs and background, subsequent blob detection, and final application of a Constant False Alarm Rate (CFAR) algorithm. The filter relies mainly on the HV-polarized intensity which often reveals a larger difference between icebergs and sea ice or open water. The blob detector identifies locations of potential icebergs and thus shortens computation time. The final detection is performed on the identified blobs using the CFAR algorithm. About 2000 icebergs captured in fast ice were visually identified in Sentinel-2 Multi Spectral Imager (MSI) data and exploited for an assessment of the detection scheme performance using confusion matrices. For our performance tests, we used four Sentinel-1 EWS images. For judging the effect of spatial resolution, we carried out an additional test with one Sentinel-1 Interferometric Wide Swath (IWS) mode image. Our results show that only 8–22 percent of the icebergs could be detected in the EWS images, and over 90 percent of all detections were false alarms. In IWS mode, the number of correctly identified icebergs increased to 38 percent. However, we obtained a larger number of false alarms in the IWS image than in the corresponding EWS image. We identified two problems for iceberg detection: 1) with the given frequency–polarization combination, not all icebergs are strong scatterers at HV-polarization, and (2) icebergs and deformation structures present on fast ice can often not be distinguished since both may reveal equally strong responses at HV-polarization.
    Repository Name: EPIC Alfred Wegener Institut
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  • 30
    Publication Date: 2019-04-23
    Description: Thermokarst lakes in the Arctic and Subarctic release carbon from thawing permafrost in the form of methane and carbon dioxide with important implications for regional and global carbon cycles. Lake ice impedes the release of gas during the winter. For instance, bubbles released from lake sediments become trapped in downward growing lake ice, resulting in vertically-oriented bubble columns in the ice that are visible on the lake surface. We here describe a classification technique using an object-based image analysis (OBIA) framework to successfully map ebullition bubbles in airborne imagery of early winter ice on an interior Alaska thermokarst lake. Ebullition bubbles appear as white patches in high-resolution optical remote sensing images of snow-free lake ice acquired in early winter and, thus, can be mapped across whole lake areas. We used high-resolution (9–11 cm) aerial images acquired two and four days following freeze-up in the years 2011 and 2012, respectively. The design of multiresolution segmentation and region-specific classification rulesets allowed the identification of bubble features and separation from other confounding factors such as snow, submerged and floating vegetation, shadows, and open water. The OBIA technique had an accuracy of 〉95% for mapping ebullition bubble patches in early winter lake ice. Overall, we mapped 1195 and 1860 ebullition bubble patches in the 2011 and 2012 images, respectively. The percent surface area of lake ice covered with ebullition bubble patches for 2011 was 2.14% and for 2012 was 2.67%, representing a conservative whole lake estimate of bubble patches compared to ground surveys usually conducted on thicker ice 10 or more days after freeze-up. Our findings suggest that the information derived from high-resolution optical images of lake ice can supplement spatially limited field sampling methods to better estimate methane flux from individual lakes. The method can also be used to improve estimates of methane ebullition from numerous lakes within larger regions.
    Repository Name: EPIC Alfred Wegener Institut
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  • 31
    facet.materialart.
    Unknown
    MDPI
    In:  EPIC3Applied Sciences, MDPI, 7(4), pp. 330-330, ISSN: 2076-3417
    Publication Date: 2017-05-22
    Description: Light microscopy analysis of diatom frustules is widely used in basic and applied research, notably taxonomy, morphometrics, water quality monitoring and paleo-environmental studies. Although there is a need for automation in these applications, various developments in image processing and analysis methodology supporting these tasks have not become widespread in diatom-based analyses. We have addressed this issue by combining our automated diatom image analysis software SHERPA with a commercial slide-scanning microscope. The resulting workflow enables mass-analyses of a broad range of morphometric features from individual frustules mounted on permanent slides. Extensive automation and internal quality control of the results helps to minimize user intervention, but care was taken to allow the user to stay in control of the most critical steps (exact segmentation of valve outlines and selection of objects of interest) using interactive functions for reviewing and revising results. In this contribution, we describe our workflow and give an overview of factors critical for success, ranging from preparation and mounting through slide scanning and autofocus finding to final morphometric data extraction. To demonstrate the usability of our methods we finally provide an example application by analysing Fragilariopsis kerguelensis valves originating from a sediment core, which substantially extends the size range reported in the literature.
    Repository Name: EPIC Alfred Wegener Institut
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  • 32
    Publication Date: 2017-06-15
    Description: We present an annotated list of new finds of red algae from Hainan Island, Southern China, including those found in 1990 and 1992 during the German-Chinese expeditions to Hainan Island and in 2008–2016 by Titlyanova, Titlyanov, and Li. Between 1990 and 1992, a total of 64 taxa of red algae were newly recorded for Hainan Island. Of these 15 species were new records for China. During the period 2008–2016, a further 54 taxa were newly recorded for Hainan Island, of which 20 were new records for China. The full list of new taxa includes taxonomic forms, dates, and locales, together with known biogeographical distributions. During both periods, the apparent enrichment of red algal marine flora has occurred in a similar way—mainly at the expense of epiphytes with filamentous, thin-filamentous, and finely branched forms. We believe that the changes in the flora of Hainan Island have been influenced by both anthropogenic and natural factors including in particular exploitation of herbivores, nutrient pollution, and coral bleaching.
    Repository Name: EPIC Alfred Wegener Institut
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  • 33
    Publication Date: 2017-07-24
    Repository Name: EPIC Alfred Wegener Institut
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  • 34
    Publication Date: 2017-05-29
    Description: Alexandrium ostenfeldii is a toxic dinoflagellate that has recently bloomed in Ouwerkerkse Kreek, The Netherlands, and which is able to cause a serious threat to shellfish consumers and aquacultures. We used a large set of 68 strains to the aim of fully characterizing the toxin profiles of the Dutch A. ostenfeldii in consideration of recent reports of novel toxins. Alexandrium ostenfeldii is known as a causative species of paralytic shellfish poisoning, and consistently in the Dutch population we determined the presence of several paralytic shellfish toxins (PST) including saxitoxin (STX), GTX2/3 (gonyautoxins), B1 and C1/C2. We also examined the production of spiroimine toxins by the Dutch A. ostenfeldii strains. An extensive liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed a high intraspecific variability of spirolides (SPX) and gymnodimines (GYM). Spirolides included 13-desMethyl-spirolide C generally as the major compound and several other mostly unknown SPX-like compounds that were detected and characterized. Besides spirolides, the presence of gymnodimine A and 12-Methyl-gymnodimine A was confirmed, together with two new gymnodimines. One of these was tentatively identified as an analogue of gymnodimine D and was the most abundant gymnodimine (calculated cell quota up to 274 pg cell−1, expressed as GYM A equivalents). Our multi-clonal approach adds new analogues to the increasing number of compounds in these toxin classes and revealed a high strain variability in cell quota and in toxin profile of toxic compounds within a single population.
    Repository Name: EPIC Alfred Wegener Institut
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  • 35
    Publication Date: 2017-07-03
    Description: Lakes are a ubiquitous landscape feature in northern permafrost regions. They have a strong impact on carbon, energy and water fluxes and can be quite responsive to climate change. The monitoring of lake change in northern high latitudes, at a sufficiently accurate spatial and temporal resolution, is crucial for understanding the underlying processes driving lake change. To date, lake change studies in permafrost regions were based on a variety of different sources, image acquisition periods and single snapshots, and localized analysis, which hinders the comparison of different regions. Here, we present a methodology based on machine-learning based classification of robust trends of multi-spectral indices of Landsat data (TM, ETM+, OLI) and object-based lake detection, to analyze and compare the individual, local and regional lake dynamics of four different study sites (Alaska North Slope, Western Alaska, Central Yakutia, Kolyma Lowland) in the northern permafrost zone from 1999 to 2014. Regional patterns of lake area change on the Alaska North Slope (−0.69%), Western Alaska (−2.82%), and Kolyma Lowland (−0.51%) largely include increases due to thermokarst lake expansion, but more dominant lake area losses due to catastrophic lake drainage events. In contrast, Central Yakutia showed a remarkable increase in lake area of 48.48%, likely resulting from warmer and wetter climate conditions over the latter half of the study period. Within all study regions, variability in lake dynamics was associated with differences in permafrost characteristics, landscape position (i.e., upland vs. lowland), and surface geology. With the global availability of Landsat data and a consistent methodology for processing the input data derived from robust trends of multi-spectral indices, we demonstrate a transferability, scalability and consistency of lake change analysis within the northern permafrost region.
    Repository Name: EPIC Alfred Wegener Institut
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  • 36
    Publication Date: 2021-08-16
    Description: In permafrost areas, seasonal freeze-thaw cycles result in upward and downward movements of the ground. For some permafrost areas, long-term downward movements were reported during the last decade. We measured seasonal and multi-year ground movements in a yedoma region of the Lena River Delta, Siberia, in 2013–2017, using reference rods installed deep in the permafrost. The seasonal subsidence was 1.7 +- 1.5 cm in the cold summer of 2013 and 4.8 +- 2 cm in the warm summer of 2014. Furthermore, we measured a pronounced multi-year net subsidence of 9.3 +- 5.7 cm from spring 2013 to the end of summer 2017. Importantly, we observed a high spatial variability of subsidence of up to 6 cm across a sub-meter horizontal scale. In summer 2013, we accompanied our field measurements with Differential Synthetic Aperture Radar Interferometry (DInSAR) on repeat-pass TerraSAR-X (TSX) data from the summer of 2013 to detect summer thaw subsidence over the same study area. Interferometry was strongly affected by a fast phase coherence loss, atmospheric artifacts, and possibly the choice of reference point. A cumulative ground movement map, built from a continuous interferogram stack, did not reveal a subsidence on the upland but showed a distinct subsidence of up to 2 cm in most of the thermokarst basins. There, the spatial pattern of DInSAR-measured subsidence corresponded well with relative surface wetness identified with the near infra-red band of a high-resolution optical image. Our study suggests that (i) although X-band SAR has serious limitations for ground movement monitoring in permafrost landscapes, it can provide valuable information for specific environments like thermokarst basins, and (ii) due to the high sub-pixel spatial variability of ground movements, a validation scheme needs to be developed and implemented for future DInSAR studies in permafrost environments.
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  • 37
    Publication Date: 2022-05-25
    Description: © The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 9 (2014): e112134, doi:10.1371/journal.pone.0112134.
    Description: Annual Emiliania huxleyi blooms (along with other coccolithophorid species) play important roles in the global carbon and sulfur cycles. E. huxleyi blooms are routinely terminated by large, host-specific dsDNA viruses, (Emiliania huxleyi Viruses; EhVs), making these host-virus interactions a driving force behind their potential impact on global biogeochemical cycles. Given projected increases in sea surface temperature due to climate change, it is imperative to understand the effects of temperature on E. huxleyi’s susceptibility to viral infection and its production of climatically active dimethylated sulfur species (DSS). Here we demonstrate that a 3°C increase in temperature induces EhV-resistant phenotypes in three E. huxleyi strains and that successful virus infection impacts DSS pool sizes. We also examined cellular polar lipids, given their documented roles in regulating host-virus interactions in this system, and propose that alterations to membrane-bound surface receptors are responsible for the observed temperature-induced resistance. Our findings have potential implications for global biogeochemical cycles in a warming climate and for deciphering the particular mechanism(s) by which some E. huxleyi strains exhibit viral resistance.
    Description: This study was supported by funding from the National Science Foundation (OCE-1061883 to KDB, BVM, and OCE-1061876 to GRD) and in part by grants from The Gordon and Betty Moore Foundation (to BVM and KDB).
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  • 38
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 10 (2015): e0124505, doi:10.1371/journal.pone.0124505.
    Description: Oceanic protist grazing at mesopelagic and bathypelagic depths, and their subsequent effects on trophic links between eukaryotes and prokaryotes, are not well constrained. Recent studies show evidence of higher than expected grazing activity by protists down to mesopelagic depths. This study provides the first exploration of protist grazing in the bathypelagic North Atlantic Deep Water (NADW). Grazing was measured throughout the water column at three stations in the South Atlantic using fluorescently-labeled prey analogues. Grazing in the deep Antarctic Intermediate water (AAIW) and NADW at all three stations removed 3.79% ± 1.72% to 31.14% ± 8.24% of the standing prokaryote stock. These results imply that protist grazing may be a significant source of labile organic carbon at certain meso- and bathypelagic depths.
    Description: Funding for the cruise was provided by the National Science Foundation (OCE-1154320) to EBK. Funding for the laboratory work was provided by contributions from the Woods Hole Oceanographic Institution Director of Research, Ocean Life Institute, and Deep Ocean Exploration Institute to VE.
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  • 39
    Publication Date: 2022-05-25
    Description: This is an open access article, free of all copyright. The definitive version was published in PLoS One 10 (2015): e0139904, doi: 10.1371/journal.pone.0139904.
    Description: The continental margin off the northeastern United States (NEUS) contains numerous, topographically complex features that increase habitat heterogeneity across the region. However, the majority of these rugged features have never been surveyed, particularly using direct observations. During summer 2013, 31 Remotely-Operated Vehicle (ROV) dives were conducted from 494 to 3271 m depth across a variety of seafloor features to document communities and to infer geological processes that produced such features. The ROV surveyed six broad-scale habitat features, consisting of shelf-breaching canyons, slope-sourced canyons, inter-canyon areas, open-slope/landslide-scar areas, hydrocarbon seeps, and Mytilus Seamount. Four previously unknown chemosynthetic communities dominated by Bathymodiolus mussels were documented. Seafloor methane hydrate was observed at two seep sites. Multivariate analyses indicated that depth and broad-scale habitat significantly influenced megafaunal coral (58 taxa), demersal fish (69 taxa), and decapod crustacean (34 taxa) assemblages. Species richness of fishes and crustaceans significantly declined with depth, while there was no relationship between coral richness and depth. Turnover in assemblage structure occurred on the middle to lower slope at the approximate boundaries of water masses found previously in the region. Coral species richness was also an important variable explaining variation in fish and crustacean assemblages. Coral diversity may serve as an indicator of habitat suitability and variation in available niche diversity for these taxonomic groups. Our surveys added 24 putative coral species and three fishes to the known regional fauna, including the black coral Telopathes magna, the octocoral Metallogorgia melanotrichos and the fishes Gaidropsarus argentatus, Guttigadus latifrons, and Lepidion guentheri. Marine litter was observed on 81% of the dives, with at least 12 coral colonies entangled in debris. While initial exploration revealed the NEUS region to be both geologically dynamic and biologically diverse, further research into the abiotic conditions and the biotic interactions that influence species abundance and distribution is needed.
    Description: Funding for the ship and ROV time was provided by NOAA’s Office of Ocean Exploration and Research with support from NOAA’s Deep Sea Coral Research and Technology Program, Northeast Initiative.
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  • 40
    facet.materialart.
    Unknown
    American Physical Society
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Physical Review E Statistical, Nonlinear, and Soft Matter Physics 92 (2015): 052128, doi: 10.1103/PhysRevE.92.052128.
    Description: Studies over the past decade have reported power-law distributions for the areas of terrestrial lakes and Arctic melt ponds, as well as fractal relationships between their areas and coastlines. Here we report similar fractal structure of ponds in a tidal flat, thereby extending the spatial and temporal scales on which such phenomena have been observed in geophysical systems. Images taken during low tide of a tidal flat in Damariscotta, Maine, reveal a well-resolved power-law distribution of pond sizes over three orders of magnitude with a consistent fractal area-perimeter relationship. The data are consistent with the predictions of percolation theory for unscreened perimeters and scale-free cluster size distributions and are robust to alterations of the image processing procedure. The small spatial and temporal scales of these data suggest this easily observable system may serve as a useful model for investigating the evolution of pond geometries, while emphasizing the generality of fractal behavior in geophysical surfaces.
    Description: This material is based upon work supported by the National Science Foundation Graduate Research Fellowship Program under Grant No. 2388357, the Gordon and Betty Moore Foundation, and the National Science Foundation, Award No. OCE-1315201.
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  • 41
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 11 (2016): e0147808, doi:10.1371/journal.pone.0147808.
    Description: The amyloid precursor protein (APP) is a causal agent in the pathogenesis of Alzheimer’s disease and is a transmembrane protein that associates with membrane-limited organelles. APP has been shown to co-purify through immunoprecipitation with a kinesin light chain suggesting that APP may act as a trailer hitch linking kinesin to its intercellular cargo, however this hypothesis has been challenged. Previously, we identified an mRNA transcript that encodes a squid homolog of human APP770. The human and squid isoforms share 60% sequence identity and 76% sequence similarity within the cytoplasmic domain and share 15 of the final 19 amino acids at the C-terminus establishing this highly conserved domain as a functionally import segment of the APP molecule. Here, we study the distribution of squid APP in extruded axoplasm as well as in a well-characterized reconstituted organelle/microtubule preparation from the squid giant axon in which organelles bind microtubules and move towards the microtubule plus-ends. We find that APP associates with microtubules by confocal microscopy and co-purifies with KI-washed axoplasmic organelles by sucrose density gradient fractionation. By electron microscopy, APP clusters at a single focal point on the surfaces of organelles and localizes to the organelle/microtubule interface. In addition, the association of APP-organelles with microtubules is an ATP dependent process suggesting that the APP-organelles contain a microtubule-based motor protein. Although a direct kinesin/APP association remains controversial, the distribution of APP at the organelle/microtubule interface strongly suggests that APP-organelles have an orientation and that APP like the Alzheimer’s protein tau has a microtubule-based function.
    Description: Research reported in this publication was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103430.
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  • 42
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 11 (2016): e0153197, doi:10.1371/journal.pone.0153197.
    Description: Benthic dinoflagellates in the genus Gambierdiscus produce the ciguatoxin precursors responsible for the occurrence of ciguatera toxicity. The prevalence of ciguatera toxins in fish has been linked to the presence and distribution of toxin-producing species in coral reef ecosystems, which is largely determined by the presence of suitable benthic habitat and environmental conditions favorable for growth. Here using single factor experiments, we examined the effects of salinity, irradiance, and temperature on growth of 17 strains of Gambierdiscus representing eight species/phylotypes (G. belizeanus, G. caribaeus, G. carolinianus, G. carpenteri, G. pacificus, G. silvae, Gambierdiscus sp. type 4–5), most of which were established from either Marakei Island, Republic of Kiribati, or St. Thomas, United States Virgin Island (USVI). Comparable to prior studies, growth rates fell within the range of 0–0.48 divisions day-1. In the salinity and temperature studies, Gambierdiscus responded in a near Gaussian, non-linear manner typical for such studies, with optimal and suboptimal growth occurring in the range of salinities of 25 and 45 and 21.0 and 32.5°C. In the irradiance experiment, no mortality was observed; however, growth rates at 55μmol photons · m-2 · s-1 were lower than those at 110–400μmol photons · m-2 · s-1. At the extremes of the environmental conditions tested, growth rates were highly variable, evidenced by large coefficients of variability. However, significant differences in intraspecific growth rates were typically found only at optimal or near-optimal growth conditions. Polynomial regression analyses showed that maximum growth occurred at salinity and temperature levels of 30.1–38.5 and 23.8–29.2°C, respectively. Gambierdiscus growth patterns varied among species, and within individual species: G. belizeanus, G. caribaeus, G. carpenteri, and G. pacificus generally exhibited a wider range of tolerance to environmental conditions, which may explain their broad geographic distribution. In contrast, G. silvae and Gambierdiscus sp. types 4–5 all displayed a comparatively narrow range of tolerance to temperature, salinity, and irradiance.
    Description: This study was funded by the National Natural Science Foundation of China (41506137); Guangxi Natural Science Foundation (2015GXNSFCA139003), Centers for Disease Control and Prevention (U01 EH000421); USFDA (F223201000060C); NOAA NOS through the CiguaHAB program (Cooperative Agreement NA11NOS4780060, NA11NOS4780028); the Lana Vento Trust and VI-EPSCoR Program (NSF award # 346483 & 081441); and a System Fund from Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education (2014BGERLXT01). Support was also provided by the Woods Hole Center for Oceans and Human Health through National Science Foundation (NSF) Grant OCE-1314642, National Institute of Environmental Health Sciences (NIEHS) Grant 1-P01-ES021923-014, as well as the China Scholarship Council.
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  • 43
    Publication Date: 2022-05-25
    Description: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
    Description: Fungal secretomes contain a wide range of hydrolytic and oxidative enzymes, including cellulases, hemicellulases, pectinases, and lignin-degrading accessory enzymes, that synergistically drive litter decomposition in the environment. While secretome studies of model organisms such as Phanerochaete chrysosporium and Aspergillus species have greatly expanded our knowledge of these enzymes, few have extended secretome characterization to environmental isolates or conducted side-by-side comparisons of diverse species. Thus, the mechanisms of carbon degradation by many ubiquitous soil fungi remain poorly understood. Here we use a combination of LC-MS/MS, genomic, and bioinformatic analyses to characterize and compare the protein composition of the secretomes of four recently isolated, cosmopolitan, Mn(II)-oxidizing Ascomycetes (Alternaria alternata SRC1lrK2f, Stagonospora sp. SRC1lsM3a, Pyrenochaeta sp. DS3sAY3a, and Paraconiothyrium sporulosum AP3s5-JAC2a). We demonstrate that the organisms produce a rich yet functionally similar suite of extracellular enzymes, with species-specific differences in secretome composition arising from unique amino acid sequences rather than overall protein function. Furthermore, we identify not only a wide range of carbohydrate-active enzymes that can directly oxidize recalcitrant carbon, but also an impressive suite of redox-active accessory enzymes that suggests a role for Fenton-based hydroxyl radical formation in indirect, non-specific lignocellulose attack. Our findings highlight the diverse oxidative capacity of these environmental isolates and enhance our understanding of the role of filamentous Ascomycetes in carbon turnover in the environment.
    Description: This work was supported by the National Science Foundation (www.nsf.gov), grant numbers EAR-1249489 and CBET-1336496, both awarded to CMH. Personal support for CAZ was also provided by Harvard University (www.harvard.edu) and by a Ford Foundation (www.fordfoundation.org) Predoctoral Fellowship administered by the National Academies.
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  • 44
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 11 (2016): e0160830, doi:10.1371/journal.pone.0160830 .
    Description: Advances in offshore wind farm (OWF) technology have recently led to their construction in coastal waters that are deep enough to be seasonally stratified. As tidal currents move past the OWF foundation structures they generate a turbulent wake that will contribute to a mixing of the stratified water column. In this study we show that the mixing generated in this way may have a significant impact on the large-scale stratification of the German Bight region of the North Sea. This region is chosen as the focus of this study since the planning of OWFs is particularly widespread. Using a combination of idealised modelling and in situ measurements, we provide order-of-magnitude estimates of two important time scales that are key to understanding the impacts of OWFs: (i) a mixing time scale, describing how long a complete mixing of the stratification takes, and (ii) an advective time scale, quantifying for how long a water parcel is expected to undergo enhanced wind farm mixing. The results are especially sensitive to both the drag coefficient and type of foundation structure, as well as the evolution of the pycnocline under enhanced mixing conditions—both of which are not well known. With these limitations in mind, the results show that OWFs could impact the large-scale stratification, but only when they occupy extensive shelf regions. They are expected to have very little impact on large-scale stratification at the current capacity in the North Sea, but the impact could be significant in future large-scale development scenarios.
    Description: Funding was provided by the Helmholtz Foundation through the Polar Regions and Coasts in the Changing Earth System II (PACES II) program.
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  • 45
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 11 (2016): e0164107, doi:10.1371/journal.pone.0164107.
    Description: Common dolphins (Delphinus delphis) are responsible for the large majority of interactions with the pole-and-line tuna fishery in the Azores but the underlying drivers remain poorly understood. In this study we investigate the influence of various environmental and fisheries-related factors in promoting the interaction of common dolphins with this fishery and estimate the resultant catch losses. We analysed 15 years of fishery and cetacean interaction data (1998–2012) collected by observers placed aboard tuna fishing vessels. Dolphins interacted in less than 3% of the fishing events observed during the study period. The probability of dolphin interaction varied significantly between years with no evident trend over time. Generalized additive modeling results suggest that fishing duration, sea surface temperature and prey abundance in the region were the most important factors explaining common dolphin interaction. Dolphin interaction had no impact on the catches of albacore, skipjack and yellowfin tuna but resulted in significantly lower catches of bigeye tuna, with a predicted median annual loss of 13.5% in the number of fish captured. However, impact on bigeye catches varied considerably both by year and fishing area. Our work shows that rates of common dolphin interaction with the pole-and-line tuna fishery in the Azores are low and showed no signs of increase over the study period. Although overall economic impact was low, the interaction may lead to significant losses in some years. These findings emphasize the need for continued monitoring and for further research into the consequences and economic viability of potential mitigation measures.
    Description: This work was funded by Fundação para a Ciência e a Tecnologia (FCT) and DRCT/SRCTE, though FEDER, the Competitiveness Factors Operational (COMPETE), QREN European Social Fund, the Portuguese Ministry for Science and Education, under research projects TRACE (PTDC/MAR/74071/2006), FCT Exploratory Project (IF/00943/2013/CP1199/CT0001), and MAPCET (M2.1.2/F/012/2011). We acknowledge the support of Fundação para a Ciência e Tecnologia (FCT), through the strategic project UID/MAR/04292/2013 granted to MARE. We thank the Azorean Regional Government for funding POPA, the Ship-owners Association and the Association of the Tuna Canning Industries for their support to the program. MJC was supported by a DRCT doctoral grant (M3.1.2/F/008/2009). MAS was supported by POPH, QREN, European Social Fund and Portuguese Ministry for Science and Education, through an FCT Investigator grant (IF/00943/2013).
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  • 46
    Publication Date: 2022-05-25
    Description: © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 13 (2018): e0205015, doi:10.1371/journal.pone.0205015.
    Description: Channelopsins and photo-regulated ion channels make it possible to use light to control electrical activity of cells. This powerful approach has lead to a veritable explosion of applications, though it is limited to changing membrane voltage of the target cells. An enormous potential could be tapped if similar opto-genetic techniques could be extended to the control of chemical signaling pathways. Photopigments from invertebrate photoreceptors are an obvious choice—as they do not bleach upon illumination -however, their functional expression has been problematic. We exploited an unusual opsin, pScop2, recently identified in ciliary photoreceptors of scallop. Phylogenetically, it is closer to vertebrate opsins, and offers the advantage of being a bi-stable photopigment. We inserted its coding sequence and a fluorescent protein reporter into plasmid vectors and demonstrated heterologous expression in various mammalian cell lines. HEK 293 cells were selected as a heterologous system for functional analysis, because wild type cells displayed the largest currents in response to the G-protein activator, GTP-γ-S. A line of HEK cells stably transfected with pScop2 was generated; after reconstitution of the photopigment with retinal, light responses were obtained in some cells, albeit of modest amplitude. In native photoreceptors pScop2 couples to Go; HEK cells express poorly this G-protein, but have a prominent Gq/PLC pathway linked to internal Ca mobilization. To enhance pScop2 competence to tap into this pathway, we swapped its third intracellular loop—important to confer specificity of interaction between 7TMDRs and G-proteins—with that of a Gq-linked opsin which we cloned from microvillar photoreceptors present in the same retina. The chimeric construct was evaluated by a Ca fluorescence assay, and was shown to mediate a robust mobilization of internal calcium in response to illumination. The results project pScop2 as a potentially powerful optogenetic tool to control signaling pathways.
    Description: This work was funded by Colciencias grant FP44842-010-2015 and Connecticut Fund for Science.
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  • 47
    Publication Date: 2022-05-25
    Description: © The Authors, 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Rogers, K. L., Bosman, S. H., Lardie-Gaylord, M., McNichol, A., Rosenheim, B. E., Montoya, J. P., & Chanton, J. P. (2019). Petrocarbon evolution: Ramped pyrolysis/oxidation and isotopic studies of contaminated oil sediments from the Deepwater Horizon oil spill in the Gulf of Mexico. PLoS One, 14(2), (2019):e0212433, doi:10.1371/journal.pone.0212433.
    Description: Hydrocarbons released during the Deepwater Horizon (DWH) oil spill weathered due to exposure to oxygen, light, and microbes. During weathering, the hydrocarbons’ reactivity and lability was altered, but it remained identifiable as “petrocarbon” due to its retention of the distinctive isotope signatures (14C and 13C) of petroleum. Relative to the initial estimates of the quantity of oil-residue deposited in Gulf sediments based on 2010–2011 data, the overall coverage and quantity of the fossil carbon on the seafloor has been attenuated. To analyze recovery of oil contaminated deep-sea sediments in the northern Gulf of Mexico we tracked the carbon isotopic composition (13C and 14C, radiocarbon) of bulk sedimentary organic carbon through time at 4 sites. Using ramped pyrolysis/oxidation, we determined the thermochemical stability of sediment organic matter at 5 sites, two of these in time series. There were clear differences between crude oil (which decomposed at a lower temperature during ramped oxidation), natural hydrocarbon seep sediment (decomposing at a higher temperature; Δ14C = -912‰) and our control site (decomposing at a moderate temperature; Δ14C = -189‰), in both the stability (ability to withstand ramped temperatures in oxic conditions) and carbon isotope signatures. We observed recovery toward our control site bulk Δ14C composition at sites further from the wellhead in ~4 years, whereas sites in closer proximity had longer recovery times. The thermographs also indicated temporal changes in the composition of contaminated sediment, with shifts towards higher temperature CO2 evolution over time at a site near the wellhead, and loss of higher temperature CO2 peaks at a more distant site.
    Description: This research was made possible by grants from The Gulf of Mexico Research Initiative through its consortiums: Ecosystem Impacts of Oil & Gas Inputs to the Gulf (ECOGIG), The Center for the Integrated Modeling and Analysis of the Gulf Ecosystem (C-Image), and Deep Sea to Coast Connectivity in the Eastern Gulf of Mexico (Deep-C) and the Resuspension, Redistribution and Deposition of DWH Recalcitrant Material (Re-Direct) project. This is ECOGIG Contribution # 521. Funding was also provided by the National Ocean Sciences Accelerator Mass Spectrometry Facility (NOSAMS) Graduate Student Internship Program (NSF OCE-1239667). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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  • 48
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 10 (2015): e0119284, doi:10.1371/journal.pone.0119284 .
    Description: Chemolithoautotrophic iron-oxidizing bacteria play an essential role in the global iron cycle. Thus far, the majority of marine iron-oxidizing bacteria have been identified as Zetaproteobacteria, a novel class within the phylum Proteobacteria. Marine iron-oxidizing microbial communities have been found associated with volcanically active seamounts, crustal spreading centers, and coastal waters. However, little is known about the presence and diversity of iron-oxidizing communities at hydrothermal systems along the slow crustal spreading center of the Mid-Atlantic Ridge. From October to November 2012, samples were collected from rust-colored mats at three well-known hydrothermal vent systems on the Mid-Atlantic Ridge (Rainbow, Trans-Atlantic Geotraverse, and Snake Pit) using the ROV Jason II. The goal of these efforts was to determine if iron-oxidizing Zetaproteobacteria were present at sites proximal to black smoker vent fields. Small, diffuse flow venting areas with high iron(II) concentrations and rust-colored microbial mats were observed at all three sites proximal to black smoker chimneys. A novel, syringe-based precision sampler was used to collect discrete microbial iron mat samples at the three sites. The presence of Zetaproteobacteria was confirmed using a combination of 16S rRNA pyrosequencing and single-cell sorting, while light micros-copy revealed a variety of iron-oxyhydroxide structures, indicating that active iron-oxidizing communities exist along the Mid-Atlantic Ridge. Sequencing analysis suggests that these iron mats contain cosmopolitan representatives of Zetaproteobacteria, but also exhibit diversity that may be uncommon at other iron-rich marine sites studied to date. A meta-analysis of publically available data encompassing a variety of aquatic habitats indicates that Zetaproteobacteria are rare if an iron source is not readily available. This work adds to the growing understanding of Zetaproteobacteria ecology and suggests that this organism is likely locally restricted to iron-rich marine environments but may exhibit wide-scale geographic distribution, further underscoring the importance of Zetaproteobacteria in global iron cycling.
    Description: This work was supported by grants from the National Science Foundation [grants OCE-0926805 (DE and JAB), OCE-1155754 (DE), and OCE-1131109 (GWL)] and the National Aeronautics and Space Administration [NNX12AG20G (GWL and DE)].
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  • 49
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 10 (2015): e0140578, doi: 10.1371/journal.pone.0140578.
    Description: Microbial communities mediate the biogeochemical cycles that drive ecosystems, and it is important to understand how these communities are affected by changing environmental conditions, especially in complex coastal zones. As fresh and marine waters mix in estuaries and river plumes, the salinity, temperature, and nutrient gradients that are generated strongly influence bacterioplankton community structure, yet, a parallel change in functional diversity has not been described. Metagenomic and metatranscriptomic analyses were conducted on five water samples spanning the salinity gradient of the Columbia River coastal margin, including river, estuary, plume, and ocean, in August 2010. Samples were pre-filtered through 3 μm filters and collected on 0.2 μm filters, thus results were focused on changes among free-living microbial communities. Results from metagenomic 16S rRNA sequences showed taxonomically distinct bacterial communities in river, estuary, and coastal ocean. Despite the strong salinity gradient observed over sampling locations (0 to 33), the functional gene profiles in the metagenomes were very similar from river to ocean with an average similarity of 82%. The metatranscriptomes, however, had an average similarity of 31%. Although differences were few among the metagenomes, we observed a change from river to ocean in the abundance of genes encoding for catabolic pathways, osmoregulators, and metal transporters. Additionally, genes specifying both bacterial oxygenic and anoxygenic photosynthesis were abundant and expressed in the estuary and plume. Denitrification genes were found throughout the Columbia River coastal margin, and most highly expressed in the estuary. Across a river to ocean gradient, the free-living microbial community followed three different patterns of diversity: 1) the taxonomy of the community changed strongly with salinity, 2) metabolic potential was highly similar across samples, with few differences in functional gene abundance from river to ocean, and 3) gene expression was highly variable and generally was independent of changes in salinity.
    Description: This study was carried out within the context of the Science and Technology Center for Coastal Margin Observation & Prediction (CMOP) supported by the National Science Foundation, grant number OCE-0424602 to Antonio Baptista (http://www.stccmop.org).
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  • 50
    Publication Date: 2022-05-25
    Description: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The definitive version was published in PLoS One 11 (2016): e0162401, doi:10.1371/journal.pone.0162401.
    Description: Heavy metals such as mercury (Hg) pose a significant health hazard through bioaccumulation and biomagnification. By penetrating cell membranes, heavy metal ions may lead to pathological conditions. Here we examined the responses of Ammonia parkinsoniana, a benthic foraminiferan, to different concentrations of Hg in the artificial sea water. Confocal images of untreated and treated specimens using fluorescent probes (Nile Red and Acridine Orange) provided an opportunity for visualizing the intracellular lipid accumulation and acidic compartment regulation. With increased Hg over time, we observed an increased number of lipid droplets, which may have acted as a detoxifying organelle where Hg is sequestered and biologically inactivated. Further, Hg seems to promote the proliferation of lysosomes both in terms of number and dimension that, at the highest level of Hg, resulted in cell death. We report, for the first time, the presence of Hg within the foraminiferal cell: at the basal part of pores, in the organic linings of the foramen/septa, and as cytoplasmic accumulations.
    Description: The research for this paper was partially made possible by the financial support from the PRIN 2010-2011 Ministero dell’Istruzione, dell’Università e della Ricerca (MIUR) (protocollo 2010RMTLYR) to RC. JMB acknowledges support from The Investment in Science Fund at WHOI. BG, JRE, AJ, LZ, and EMP were supported in part by the Office of Biological and Environmental Research, US Department of Energy (DOE) as part of the Mercury Science Focus Area at Oak Ridge National Laboratory, which is managed by UT-Battelle LLC for the DOE under contract DE-AC05-00OR22725.
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  • 51
    Publication Date: 2022-05-25
    Description: © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLos One 13 (2018): e0200386, doi:10.1371/journal.pone.0200386.
    Description: Soft robotics is an emerging technology that has shown considerable promise in deep-sea marine biological applications. It is particularly useful in facilitating delicate interactions with fragile marine organisms. This study describes the shipboard design, 3D printing and integration of custom soft robotic manipulators for investigating and interacting with deep-sea organisms. Soft robotics manipulators were tested down to 2224m via a Remotely-Operated Vehicle (ROV) in the Phoenix Islands Protected Area (PIPA) and facilitated the study of a diverse suite of soft-bodied and fragile marine life. Instantaneous feedback from the ROV pilots and biologists allowed for rapid re-design, such as adding “fingernails”, and re-fabrication of soft manipulators at sea. These were then used to successfully grasp fragile deep-sea animals, such as goniasterids and holothurians, which have historically been difficult to collect undamaged via rigid mechanical arms and suction samplers. As scientific expeditions to remote parts of the world are costly and lengthy to plan, on-the-fly soft robot actuator printing offers a real-time solution to better understand and interact with delicate deep-sea environments, soft-bodied, brittle, and otherwise fragile organisms. This also offers a less invasive means of interacting with slow-growing deep marine organisms, some of which can be up to 18,000 years old.
    Description: This work is supported by NOAA OER Grant # NA17OAR0110083 “Exploration of the Seamounts of the Phoenix Islands Protected Area” to RDR, EEC, TMS and DFG and Schmidt Ocean Institute Grant: “What is the Current State of the Deep-Sea Coral Ecosystem in the Phoenix Island Protected Area?” to EEC, RDR, TMS and DFG; NSF Instrument Development for Biological Research Award # 1556164 to RJW and #1556123 to DFG; the National Academies Keck Futures Initiative of the National Academy of Sciences under award #NAKFI DBS21 to RJW and DFG; and NFS Research Fellowship awarded to KPB (#DGE1144152). It is also supported by the Wyss Institute for Biologically Inspired Engineering at Harvard University. We are grateful for the support from the National Geographic Society Innovation Challenge (Grant No.: SP 12-14) to RJW and DFG.
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  • 52
    Publication Date: 2022-05-25
    Description: © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 13 (2018): e0207532, doi:10.1371/journal.pone.0207532.
    Description: Acoustic standing waves can precisely focus flowing particles or cells into tightly positioned streams for interrogation or downstream separations. The efficiency of an acoustic standing wave device is dependent upon operating at a resonance frequency. Small changes in a system’s temperature and sample salinity can shift the device’s resonance condition, leading to poor focusing. Practical implementation of an acoustic standing wave system requires an automated resonance control system to adjust the standing wave frequency in response to environmental changes. Here we have developed a rigorous approach for quantifying the optimal acoustic focusing frequency at any given environmental condition. We have demonstrated our approach across a wide range of temperature and salinity conditions to provide a robust characterization of how the optimal acoustic focusing resonance frequency shifts across these conditions. To generalize these results, two microfluidic bulk acoustic standing wave systems (a steel capillary and an etched silicon wafer) were examined. Models of these temperature and salinity effects suggest that it is the speed of sound within the liquid sample that dominates the resonance frequency shift. Using these results, a simple reference table can be generated to predict the optimal resonance condition as a function of temperature and salinity. Additionally, we show that there is a local impedance minimum associated with the optimal system resonance. The integration of the environmental results for coarse frequency tuning followed by a local impedance characterization for fine frequency adjustments, yields a highly accurate method of resonance control. Such an approach works across a wide range of environmental conditions, is easy to automate, and could have a significant impact across a wide range of microfluidic acoustic standing wave systems.
    Description: This research was supported by grants from the National Institute of General Medical Sciences of the National Institutes of Health under award number R21GM107805 and the NSF under award number (OCE-1130140 and OCE-1131134) to SWG, RJO, and HMS.
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  • 53
    Publication Date: 2022-05-25
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Sathyendranath, S., Brewin, R. J. W., Brockmann, C., Brotas, V., Calton, B., Chuprin, A., Cipollini, P., Couto, A. B., Dingle, J., Doerffer, R., Donlon, C., Dowell, M., Farman, A., Grant, M., Groom, S., Horseman, A., Jackson, T., Krasemann, H., Lavender, S., Martinez-Vicente, V., Mazeran, C., Melin, F., Moore, T. S., Muller, D., Regner, P., Roy, S., Steele, C. J., Steinmetz, F., Swinton, J., Taberner, M., Thompson, A., Valente, A., Zuhlke, M., Brando, V. E., Feng, H., Feldman, G., Franz, B. A., Frouin, R., Gould, R. W., Hooker, S. B., Kahru, M., Kratzer, S., Mitchell, B. G., Muller-Karger, F. E., Sosik, H. M., Voss, K. J., Werdell, J., & Platt, T. An ocean-colour time series for use in climate studies: The experience of the ocean-colour climate change initiative (OC-CCI). Sensors, 19(19), (2019): 4285, doi: 10.3390/s19194285.
    Description: Ocean colour is recognised as an Essential Climate Variable (ECV) by the Global Climate Observing System (GCOS); and spectrally-resolved water-leaving radiances (or remote-sensing reflectances) in the visible domain, and chlorophyll-a concentration are identified as required ECV products. Time series of the products at the global scale and at high spatial resolution, derived from ocean-colour data, are key to studying the dynamics of phytoplankton at seasonal and inter-annual scales; their role in marine biogeochemistry; the global carbon cycle; the modulation of how phytoplankton distribute solar-induced heat in the upper layers of the ocean; and the response of the marine ecosystem to climate variability and change. However, generating a long time series of these products from ocean-colour data is not a trivial task: algorithms that are best suited for climate studies have to be selected from a number that are available for atmospheric correction of the satellite signal and for retrieval of chlorophyll-a concentration; since satellites have a finite life span, data from multiple sensors have to be merged to create a single time series, and any uncorrected inter-sensor biases could introduce artefacts in the series, e.g., different sensors monitor radiances at different wavebands such that producing a consistent time series of reflectances is not straightforward. Another requirement is that the products have to be validated against in situ observations. Furthermore, the uncertainties in the products have to be quantified, ideally on a pixel-by-pixel basis, to facilitate applications and interpretations that are consistent with the quality of the data. This paper outlines an approach that was adopted for generating an ocean-colour time series for climate studies, using data from the MERIS (MEdium spectral Resolution Imaging Spectrometer) sensor of the European Space Agency; the SeaWiFS (Sea-viewing Wide-Field-of-view Sensor) and MODIS-Aqua (Moderate-resolution Imaging Spectroradiometer-Aqua) sensors from the National Aeronautics and Space Administration (USA); and VIIRS (Visible and Infrared Imaging Radiometer Suite) from the National Oceanic and Atmospheric Administration (USA). The time series now covers the period from late 1997 to end of 2018. To ensure that the products meet, as well as possible, the requirements of the user community, marine-ecosystem modellers, and remote-sensing scientists were consulted at the outset on their immediate and longer-term requirements as well as on their expectations of ocean-colour data for use in climate research. Taking the user requirements into account, a series of objective criteria were established, against which available algorithms for processing ocean-colour data were evaluated and ranked. The algorithms that performed best with respect to the climate user requirements were selected to process data from the satellite sensors. Remote-sensing reflectance data from MODIS-Aqua, MERIS, and VIIRS were band-shifted to match the wavebands of SeaWiFS. Overlapping data were used to correct for mean biases between sensors at every pixel. The remote-sensing reflectance data derived from the sensors were merged, and the selected in-water algorithm was applied to the merged data to generate maps of chlorophyll concentration, inherent optical properties at SeaWiFS wavelengths, and the diffuse attenuation coefficient at 490 nm. The merged products were validated against in situ observations. The uncertainties established on the basis of comparisons with in situ data were combined with an optical classification of the remote-sensing reflectance data using a fuzzy-logic approach, and were used to generate uncertainties (root mean square difference and bias) for each product at each pixel.
    Description: This work was funded by the Ocean Colour Climate Change initiative of the European Space Agency (Grant Number 4000101437/10/I-LG). We acknowledge additional funding support by NERC through the National Centre for Earth Observation (Grant Number PR140015). Additional funding from a Simons Foundation Grant (549947, SS) is also gratefully acknowledged. V.B. also acknowledges funding from the European Union’s Horizon 2020 Research and Innovation Programme grant agreement N_ 810139: Project Portugal Twinning for Innovation and Excellence in Marine Science and Earth Observation – PORTWIMS.
    Keywords: ocean colour ; water-leaving radiance ; remote-sensing reflectance ; phytoplankton ; chlorophyll-a ; inherent optical properties ; Climate Change Initiative ; optical water classes ; Essential Climate Variable ; uncertainty characterisation
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  • 54
    Publication Date: 2022-05-25
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 14(1), (2019):e0204193, doi: 10.1371/journal.pone.0204193.
    Description: The resilience of regeneration in vertebrates is not very well understood. Yet understanding if tissues can regenerate after repeated insults, and identifying limitations, is important for elucidating the underlying mechanisms of tissue plasticity. This is particularly challenging in tissues, such as the nervous system, which possess a large number of terminally differentiated cells and often exhibit limited regeneration in the first place. However, unlike mammals, which exhibit very limited regeneration of spinal cord tissues, many non-mammalian vertebrates, including lampreys, bony fishes, amphibians, and reptiles, regenerate their spinal cords and functionally recover even after a complete spinal cord transection. It is well established that lampreys undergo full functional recovery of swimming behaviors after a single spinal cord transection, which is accompanied by tissue repair at the lesion site, as well as axon and synapse regeneration. Here we begin to explore the resilience of spinal cord regeneration in lampreys after a second spinal transection (re-transection). We report that by all functional and anatomical measures tested, lampreys regenerate after spinal re-transection just as robustly as after single transections. Recovery of swimming, synapse and cytoskeletal distributions, axon regeneration, and neuronal survival were nearly identical after spinal transection or re-transection. Only minor differences in tissue repair at the lesion site were observed in re-transected spinal cords. Thus, regenerative potential in the lamprey spinal cord is largely unaffected by spinal re-transection, indicating a greater persistent regenerative potential than exists in some other highly regenerative models. These findings establish a new path for uncovering pro-regenerative targets that could be deployed in non-regenerative conditions.
    Description: The authors would like to thank Dr. Cristina Roman-Vendrell and Louie Kerr, Director of the Central Microscopy Facility at the MBL, for technical support. We also thank Dr. Juan Diaz-Quiroz for helpful comments on the manuscript. EG was supported in part by an NSF REU Award (#1659604: Biological Discovery in Woods Hole at the Marine Biological Laboratory).
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  • 55
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 10 (2015): e0133963, doi: 10.1371/journal.pone.0133963.
    Description: The Western clawed frog, Xenopus tropicalis, is a highly promising model amphibian, especially in developmental and physiological research, and as a tool for understanding disease. It was originally found in the West African rainforest belt, and was introduced to the research community in the 1990s. The major strains thus far known include the Nigerian and Ivory Coast strains. However, due to its short history as an experimental animal, the genetic relationship among the various strains has not yet been clarified, and establishment of inbred strains has not yet been achieved. Since 2003 the Institute for Amphibian Biology (IAB), Hiroshima University has maintained stocks of multiple X. tropicalis strains and conducted consecutive breeding as part of the National BioResource Project. In the present study we investigated the inbreeding ratio and genetic relationship of four inbred strains at IAB, as well as stocks from other institutions, using highly polymorphic microsatellite markers and mitochondrial haplotypes. Our results show successive reduction of heterozygosity in the genome of the IAB inbred strains. The Ivory Coast strains clearly differed from the Nigerian strains genetically, and three subgroups were identified within both the Nigerian and Ivory Coast strains. It is noteworthy that the Ivory Coast strains have an evolutionary divergent genetic background. Our results serve as a guide for the most effective use of X. tropicalis strains, and the long-term maintenance of multiple strains will contribute to further research efforts.
    Description: This work was supported by grants from the Wellcome Trust (101480/Z/13/Z, http://www.wellcome.ac.uk/stellent/group​s/corporatesite/@msh_publishing_group/do​cuments/web_document/wts058331.pdf) and Biotechnology and Biological Sciences Research Council (BB/K019988/1, http://www.bbsrc.ac.uk/pa/grants/AwardDe​tails.aspx?FundingReference=BB/K019988/1) to the European Xenopus Resource Centre. This work was also supported by a Grant-in-Aid for Young Scientists (B) (No. 23710282, http://kaken.nii.ac.jp/d/p/23710282.en.h​tml) to TI from the Ministry of Education, Culture, Sports, Science and Technology, Japan and a Grant-in-Aid for Scientific Research (B) (No. 20510216, http://kaken.nii.ac.jp/d/p/24310173.en.h​tml) to MS from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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  • 56
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One (10): e0141842, doi:10.1371/journal.pone.0141842.
    Description: Differences in the bacterial community structure associated with 7 skin sites in 71 healthy people over five days showed significant correlations with age, gender, physical skin parameters, and whether participants lived in urban or rural locations in the same city. While body site explained the majority of the variance in bacterial community structure, the composition of the skin-associated bacterial communities were predominantly influenced by whether the participants were living in an urban or rural environment, with a significantly greater relative abundance of Trabulsiella in urban populations. Adults maintained greater overall microbial diversity than adolescents or the elderly, while the intragroup variation among the elderly and rural populations was significantly greater. Skin-associated bacterial community structure and composition could predict whether a sample came from an urban or a rural resident ~5x greater than random.
    Description: This work was supported by a grant from Johnson & Johnson (China).
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    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 10 (2015): e0143299, doi:10.1371/journal.pone.0143299.
    Description: For phytoplankton and other microbes, nutrient receptors are often the passages through which viruses invade. This presents a bottom-up vs. top-down, co-limitation scenario; how do these would-be-hosts balance minimizing viral susceptibility with maximizing uptake of limiting nutrient(s)? This question has been addressed in the biological literature on evolutionary timescales for populations, but a shorter timescale, mechanistic perspective is lacking, and marine viral literature suggests the strong influence of additional factors, e.g. host size; while the literature on both nutrient uptake and host-virus interactions is expansive, their intersection, of ubiquitous relevance to marine environments, is understudied. I present a simple, mechanistic model from first principles to analyze the effect of this co-limitation scenario on individual growth, which suggests that in environments with high risk of viral invasion or spatial/temporal heterogeneity, an individual host’s growth rate may be optimized with respect to receptor coverage, producing top-down selective pressure on short timescales. The model has general applicability, is suggestive of hypotheses for empirical exploration, and can be extended to theoretical studies of more complex behaviors and systems.
    Description: This work was supported by the Massachusetts Institute of Technology Charles Vest Presidential Fellowship.
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    Publication Date: 2022-05-25
    Description: © American Chemical Society, 2015. This article is posted here by permission of American Chemical Society; copying and redistribution for non-commercial research and education purposes only. The definitive version was published in ACS Nano 10 (2016): 6-37, doi:10.1021/acsnano.5b07826.
    Description: The microbiome presents great opportunities for understanding and improving the world around us and elucidating the interactions that compose it. The microbiome also poses tremendous challenges for mapping and manipulating the entangled networks of interactions among myriad diverse organisms. Here, we describe the opportunities, technical needs, and potential approaches to address these challenges, based on recent and upcoming advances in measurement and control at the nanoscale and beyond. These technical needs will provide the basis for advancing the largely descriptive studies of the microbiome to the theoretical and mechanistic understandings that will underpin the discipline of microbiome engineering. We anticipate that the new tools and methods developed will also be more broadly useful in environmental monitoring, medicine, forensics, and other areas.
    Description: This research was supported by the Office of Naval Research Grant #N000141410051 (P.S.W., G.C.L.W., and T.Y.), the Genomic Science Program of the U.S. DOE-OBER,
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  • 59
    Publication Date: 2022-05-25
    Description: © The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 12 (2017): e0173350, doi:10.1371/journal.pone.0173350.
    Description: Scleractinian coral are experiencing unprecedented rates of mortality due to increases in sea surface temperatures in response to global climate change. Some coral species however, survive high temperature events due to a reduced susceptibility to bleaching. We investigated the relationship between bleaching susceptibility and expression of five metabolically related genes of Symbiodinium spp. from the coral Porites astreoides originating from an inshore and offshore reef in the Florida Keys. The acclimatization potential of Symbiodinium spp. to changing temperature regimes was also measured via a two-year reciprocal transplant between the sites. Offshore coral fragments displayed significantly higher expression in Symbiodinium spp. genes PCNA, SCP2, G3PDH, PCP and psaE than their inshore counterparts (p〈0.05), a pattern consistent with increased bleaching susceptibility in offshore corals. Additionally, gene expression patterns in Symbiodinium spp. from site of origin were conserved throughout the two-year reciprocal transplant, indicating acclimatization did not occur within this multi-season time frame. Further, laboratory experiments were used to investigate the influence of acute high temperature (32°C for eight hours) and disease (lipopolysaccharide of Serratia marcescens) on the five metabolically related symbiont genes from the same offshore and inshore P. astreoides fragments. Gene expression did not differ between reef fragments, or as a consequence of acute exposure to heat or heat and disease, contrasting to results found in the field. Gene expression reported here indicates functional variation in populations of Symbiodinium spp. associated with P. astreoides in the Florida Keys, and is likely a result of localized adaptation. However, gene expression patterns observed in the lab imply that functional variation in zooxanthellae observed under conditions of chronic moderate stress is lost under the acute extreme conditions studied here.
    Description: Funding for this research was provided by Coastal Preservation network (KBS, BHS).
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  • 60
    Publication Date: 2022-05-25
    Description: © The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 12 (2017): e0179318, doi: 10.1371/journal.pone.0179318.
    Description: Trace metals are essential for health but toxic when present in excess. The maintenance of trace metals at physiologic levels reflects both import and export by cells and absorption and excretion by organs. The mechanism by which this maintenance is achieved in vertebrate organisms is incompletely understood. To explore this, we chose zebrafish as our model organism, as they are amenable to both pharmacologic and genetic manipulation and comprise an ideal system for genetic screens and toxicological studies. To characterize trace metal content in developing zebrafish, we measured levels of three trace elements, copper, zinc, and manganese, from the oocyte stage to 30 days post-fertilization using inductively coupled plasma mass spectrometry. Our results indicate that metal levels are stable until zebrafish can acquire metals from the environment and imply that the early embryo relies on maternal contribution of metals to the oocyte. We also measured metal levels in bodies and yolks of embryos reared in presence and absence of the copper chelator neocuproine. All three metals exhibited different relative abundances between yolks and bodies of embryos. While neocuproine treatment led to an expected phenotype of copper deficiency, total copper levels were unaffected, indicating that measurement of total metal levels does not equate with measurement of biologically active metal levels. Overall, our data not only can be used in the design and execution of genetic, physiologic, and toxicologic studies but also has implications for the understanding of vertebrate metal homeostasis.
    Description: This work was supported by the National Institutes of Health, R00 DK84122.
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  • 61
    Publication Date: 2022-05-25
    Description: Author Posting. © American Chemical Society, 2018. This is an open access article published under an ACS AuthorChoice License. The definitive version was published in Environmental Science and Technology Letters 5 (2018): 226–231, doi:10.1021/acs.estlett.8b00084.
    Description: Chemical dispersants are one of many tools used to mitigate the overall environmental impact of oil spills. In principle, dispersants break up floating oil into small droplets that disperse into the water column where they are subject to multiple fate and transport processes. The effectiveness of dispersants typically decreases as oil weathers in the environment. This decrease in effectiveness is often attributed to evaporation and emulsification, with the contribution of photochemical weathering assumed to be negligible. Here, we aim to test this assumption using Macondo well oil released during the Deepwater Horizon spill as a case study. Our results indicate that the effects of photochemical weathering on Deepwater Horizon oil properties and dispersant effectiveness can greatly outweigh the effects of evaporative weathering. The decrease in dispersant effectiveness after light exposure was principally driven by the decreased solubility of photo-oxidized crude oil residues in the solvent system that comprises COREXIT EC9500A. Kinetic modeling combined with geospatial analysis demonstrated that a considerable fraction of aerial applications targeting Deepwater Horizon surface oil had low dispersant effectiveness. Collectively, the results of this study challenge the paradigm that photochemical weathering has a negligible impact on the effectiveness of oil spill response and provide critical insights into the “window of opportunity” to apply chemical dispersants in response to oil spills in sunlit waters.
    Description: This work was supported, in part, by National Science Foundation Grant OCE-1333148, Gulf of Mexico Research Initiative Grants 015, SA 16-30, the DEEP-C consortium, and the Clark Family Foundation, Inc. EPA funding was provided to R.N.C. from the Oil Spill Liability Trust Fund.
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  • 62
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 10 (2015): e0117193, doi:10.1371/journal.pone.0117193.
    Description: The article reports the radiocarbon investigation results of the Lebombo Eco Trail tree, a representative African baobab from Mozambique. Several wood samples collected from the large inner cavity and from the outer part of the tree were investigated by AMS radiocarbon dating. According to dating results, the age values of all samples increase from the sampling point with the distance into the wood. For samples collected from the cavity walls, the increase of age values with the distance into the wood (up to a point of maximum age) represents a major anomaly. The only realistic explanation for this anomaly is that such inner cavities are, in fact, natural empty spaces between several fused stems disposed in a ring-shaped structure. We named them false cavities. Several important differences between normal cavities and false cavities are presented. Eventually, we dated other African baobabs with false inner cavities. We found that this new architecture enables baobabs to reach large sizes and old ages. The radiocarbon date of the oldest sample was 1425 ± 24 BP, which corresponds to a calibrated age of 1355 ± 15 yr. The dating results also show that the Lebombo baobab consists of five fused stems, with ages between 900 and 1400 years; these five stems build the complete ring. The ring and the false cavity closed 800–900 years ago. The results also indicate that the stems stopped growing toward the false cavity over the past 500 years.
    Description: The research was fully funded by the Romanian Ministry of National Education CNCS-UEFISCDI under grant PN-II-ID-PCE-2013-76.
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  • 63
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This is an open access article, free of all copyright. The definitive version was published in PLoS One 10 (2015): e0124145, doi:10.1371/journal.pone.0124145.
    Description: Ocean acidification, the progressive change in ocean chemistry caused by uptake of atmospheric CO2, is likely to affect some marine resources negatively, including shellfish. The Atlantic sea scallop (Placopecten magellanicus) supports one of the most economically important single-species commercial fisheries in the United States. Careful management appears to be the most powerful short-term factor affecting scallop populations, but in the coming decades scallops will be increasingly influenced by global environmental changes such as ocean warming and ocean acidification. In this paper, we describe an integrated assessment model (IAM) that numerically simulates oceanographic, population dynamic, and socioeconomic relationships for the U.S. commercial sea scallop fishery. Our primary goal is to enrich resource management deliberations by offering both short- and long-term insight into the system and generating detailed policy-relevant information about the relative effects of ocean acidification, temperature rise, fishing pressure, and socioeconomic factors on the fishery using a simplified model system. Starting with relationships and data used now for sea scallop fishery management, the model adds socioeconomic decision making based on static economic theory and includes ocean biogeochemical change resulting from CO2 emissions. The model skillfully reproduces scallop population dynamics, market dynamics, and seawater carbonate chemistry since 2000. It indicates sea scallop harvests could decline substantially by 2050 under RCP 8.5 CO2 emissions and current harvest rules, assuming that ocean acidification affects P. magellanicus by decreasing recruitment and slowing growth, and that ocean warming increases growth. Future work will explore different economic and management scenarios and test how potential impacts of ocean acidification on other scallop biological parameters may influence the social-ecological system. Future empirical work on the effect of ocean acidification on sea scallops is also needed.
    Description: Cooley, Rheuban, and Doney were supported by NOAA Grant NA12NOS4780145 (www.noaa.gov) and the Center for Climate and Energy Decision Making (CEDM, NSF SES-0949710) (www.nsf.gov). Luu was supported by a WHOI Summer Student Fellowship (www.whoi.edu).
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  • 64
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 10 (2015): e0129719, doi: 10.1371/journal.pone.0129719.
    Description: We applied a series of selective antibodies for labeling the various cell types in the mammalian retina. These were used to identify the progressive loss of neurons in the FVB/N mouse, a model of early onset retinal degeneration produced by a mutation in the pde6b gene. The immunocytochemical studies, together with electroretinogram (ERG) recordings, enabled us to examine the time course of the degenerative changes that extended from the photoreceptors to the ganglion cells at the proximal end of the retina. Our study indicates that photoreceptors in FVB/N undergo a rapid degeneration within three postnatal weeks, and that there is a concomitant loss of retinal neurons in the inner nuclear layer. Although the loss of rods was detected at an earlier age during which time M- and S-opsin molecules were translocated to the cone nuclei; by 6 months all cones had also degenerated. Neuronal remodeling was also seen in the second-order neurons with horizontal cells sprouting processes proximally and dendritic retraction in rod-driven bipolar cells. Interestingly, the morphology of cone-driven bipolar cells were affected less by the disease process. The cellular structure of inner retinal neurons, i.e., ChAT amacrine cells, ganglion cells, and melanopsin-positive ganglion cells did not exhibit any gross changes of cell densities and appeared to be relatively unaffected by the massive photoreceptor degeneration in the distal retina. However, Muller cell processes began to express GFAP at their endfeet at p14, and it climbed progressively to the cell’s distal ends by 6 months. Our study indicates that FVB/N mouse provides a useful model with which to assess possible intervention strategies to arrest photoreceptor death in related diseases.
    Description: This study was supported by grants from the National Science Foundation (NSF, IOS-1021646, WS) and the National Eye Institute (NEI, EY 14161, WS).
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  • 65
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 11 (2016): e0146977, doi:10.1371/journal.pone.0146977 .
    Description: The article reports the radiocarbon investigation of Anzapalivoro, the largest za baobab (Adansonia za) specimen of Madagascar and of another za, namely the Big cistern baobab. Several wood samples collected from the large inner cavity and from the outer part/exterior of the tree were investigated by AMS (accelerator mass spectrometry) radiocarbon dating. For samples collected from the cavity walls, the age values increase with the distance into the wood up to a point of maximum age, after which the values decrease toward the outer part. This anomaly of age sequences indicates that the inner cavity of Anzapalivoro is a false cavity, practically an empty space between several fused stems disposed in a ring-shaped structure. The radiocarbon date of the oldest sample was 780 ± 30 bp, which corresponds to a calibrated age of around 735 yr. Dating results indicate that Anzapalivoro has a closed ring-shaped structure, which consists of 5 fused stems that close a false cavity. The oldest part of the biggest za baobab has a calculated age of 900 years. We also disclose results of the investigation of a second za baobab, the Big cistern baobab, which was hollowed out for water storage. This specimen, which consists of 4 fused stems, was found to be around 260 years old.
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  • 66
    Publication Date: 2022-05-25
    Description: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The definitive version was published in PLoS One 11 (2016): e0154208, doi: 10.1371/journal.pone.0154208
    Description: Some species of butterflyfish have had preyed upon corals for millions of years, yet the mechanism of butterflyfish specialized coral feeding strategy remains poorly understood. Certain butterflyfish have the ability to feed on allelochemically rich soft corals, e.g. Sinularia maxima. Cytochrome P450 (CYP) is the predominant enzyme system responsible for the detoxification of dietary allelochemicals. CYP2-like and CYP3A-like content have been associated with butterflyfish that preferentially consumes allelochemically rich soft corals. To investigate the role of butterflyfish CYP2 and CYP3A enzymes in dietary preference, we conducted oral feeding experiments using homogenates of S. maxima and a toxin isolated from the coral in four species of butterflyfish with different feeding strategies. After oral exposure to the S. maxima toxin 5-episinulaptolide (5ESL), which is not normally encountered in the Hawaiian butterflyfish diet, an endemic specialist, Chaetodon multicinctus experienced 100% mortality compared to a generalist, Chaetodon auriga, which had significantly more (3–6 fold higher) CYP3A-like basal content and catalytic activity. The specialist, Chaetodon unimaculatus, which preferentially feed on S. maxima in Guam, but not in Hawaii, had 100% survival, a significant induction of 8–12 fold CYP3A-like content, and an increased ability (2-fold) to metabolize 5ESL over other species. Computer modeling data of CYP3A4 with 5ESL were consistent with microsomal transformation of 5ESL to a C15-16 epoxide from livers of C. unimaculatus. Epoxide formation correlated with CYP3A-like content, catalytic activity, induction, and NADPH-dependent metabolism of 5ESL. These results suggest a potentially important role for the CYP3A family in butterflyfish-coral diet selection through allelochemical detoxification.
    Description: This work received support from the following sources: Resource Allocation Program of the Agricultural Research Station for UCR to DS; Summer funding by Hilda and George Liebig Environmental Sciences Summer Fellowship and travel grant Albert March Environmental Sciences Scholarship from the University of California, Riverside; and the Chemistry and DMPK CORE of COBRE, P20GM104932 from the National Institute of General Medical Sciences (NIGMS), a component of the National Institutes of Health (NIH) supported the chemistry studies.
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    Publication Date: 2022-05-25
    Description: This is an open access article, free of all copyright. The definitive version was published in PLoS ONE 11 (2016): e0164979, doi: 10.1371/journal.pone.0164979.
    Description: Understanding and managing dynamic coastal landscapes for beach-dependent species requires biological and geological data across the range of relevant environments and habitats. It is difficult to acquire such information; data often have limited focus due to resource constraints, are collected by non-specialists, or lack observational uniformity. We developed an open-source smartphone application called iPlover that addresses these difficulties in collecting biogeomorphic information at piping plover (Charadrius melodus) nest sites on coastal beaches. This paper describes iPlover development and evaluates data quality and utility following two years of collection (n = 1799 data points over 1500 km of coast between Maine and North Carolina, USA). We found strong agreement between field user and expert assessments and high model skill when data were used for habitat suitability prediction. Methods used here to develop and deploy a distributed data collection system have broad applicability to interdisciplinary environmental monitoring and modeling.
    Description: This work was supported by the North Atlantic Landscape Conservation Cooperative through the U.S. Department of the Interior Hurricane Sandy recovery program under the Disaster Relief Appropriations Act of 2013, and the U.S. Geological Survey Coastal and Marine Geology Program.
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  • 68
    Publication Date: 2022-05-25
    Description: The work is made available under the Creative Commons CCO public domain dedication.. The definitive version was published in PLoS Biology 16 (2018): e2006333, doi:10.1371/journal.pbio.2006333.
    Description: Our current understanding of biology is heavily based on a small number of genetically tractable model organisms. Most eukaryotic phyla lack such experimental models, and this limits our ability to explore the molecular mechanisms that ultimately define their biology, ecology, and diversity. In particular, marine protists suffer from a paucity of model organisms despite playing critical roles in global nutrient cycles, food webs, and climate. To address this deficit, an initiative was launched in 2015 to foster the development of ecologically and taxonomically diverse marine protist genetic models. The development of new models faces many barriers, some technical and others institutional, and this often discourages the risky, long-term effort that may be required. To lower these barriers and tackle the complexity of this effort, a highly collaborative community-based approach was taken. Herein, we describe this approach, the advances achieved, and the lessons learned by participants in this novel community-based model for research.
    Description: The research efforts, connections, and collaborations described in this paper and protocols.io (https://www.protocols.io/) were supported by the Gordon and Betty Moore Foundation’s Marine Microbiology Initiative.
    Repository Name: Woods Hole Open Access Server
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  • 69
    Publication Date: 2019-07-17
    Description: The low temperatures of polar regions and high-altitude environments, especially icy habitats, present challenges for many microorganisms. Their ability to live under subfreezing conditions implies the production of compounds conferring cryotolerance. Colwellia psychrerythraea 34H, a γ-proteobacterium isolated from subzero Arctic marine sediments, provides a model for the study of life in cold environments. We report here the identification and detailed molecular primary and secondary structures of capsular polysaccharide from C. psychrerythraea 34H cells. The polymer was isolated in the water layer when cells were extracted by phenol/water and characterized by one- and two-dimensional NMR spectroscopy together with chemical analysis. Molecular mechanics and dynamics calculations were also performed. The polysaccharide consists of a tetrasaccharidic repeating unit containing two amino sugars and two uronic acids bearing threonine as substituent. The structural features of this unique polysaccharide resemble those present in antifreeze proteins and glycoproteins. These results suggest a possible correlation between the capsule structure and the ability of C. psychrerythraea to colonize subfreezing marine environments.
    Repository Name: EPIC Alfred Wegener Institut
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  • 70
    Publication Date: 2020-02-27
    Description: The concept of multi-use of the sea has gained popularity in recent years as a result of ocean space (coastal areas and regions with relatively small sea space in particular) becoming increasingly crowded due to the development of the maritime economy. Competing claims for space can be a source of conflict, however this may also lead to mutual benefits for different users when sustainable combinations are sought. Despite increasing European-wide efforts, on-the-ground knowledge and practice of multi-use are still limited. Therefore, with the aim of investigating opportunities for multi-use development in the European seas, 10 case studies were selected, involving different site-specific contexts. This study analyses the characteristics and development potential for ocean multi-use, integrating results from desk analysis and stakeholder perceptions from different sectors in each of the case study locations. Similarities and differences between various combinations of sea uses are also identified. The results show a high heterogeneity of multi-use opportunities between case studies, with a range of combinations identified. The investigated combinations of maritime uses share an overall balance between factors promoting (drivers) and hindering (barriers) multi-use development. Based on stakeholder opinions, expected benefits (added values) of multi-use implementation outweigh potential negative impacts. Management actions are also proposed to further exploit multi-use potential at a local, regional (sub-national) and national levels.
    Repository Name: EPIC Alfred Wegener Institut
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  • 71
    facet.materialart.
    Unknown
    MDPI
    In:  EPIC3Atmosphere, MDPI, 10(12), ISSN: 2073-4433
    Publication Date: 2020-04-14
    Description: The heat imbalance is the fundamental driver for the atmospheric circulation. Therefore, it is crucially important to understand how it responds to global warming. In this study, the role of the ocean in reshaping the atmospheric meridional heat imbalance is explored based on observations and climate simulations. We found that ocean tends to strengthen the meridional heat imbalance over the mid-latitudes. This is primarily because of the uneven ocean heat uptake between the subtropical and subpolar oceans. Under global warming, the subtropical ocean absorbs relatively less heat as the water there is well stratified. In contrast, the subpolar ocean is the primary region where the ocean heat uptake takes place, because the subpolar ocean is dominated by upwelling, strong mixing, and overturning circulation. We propose that the enhanced meridional heat imbalance may potentially contribute to strengthening the water cycle, westerlies, jet stream, and mid-latitude storms.
    Repository Name: EPIC Alfred Wegener Institut
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  • 72
    Publication Date: 2022-05-26
    Description: © The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 9 (2014): e83249, doi:10.1371/journal.pone.0083249.
    Description: Knowledge of the habitat use and migration patterns of large sharks is important for assessing the effectiveness of large predator Marine Protected Areas (MPAs), vulnerability to fisheries and environmental influences, and management of shark–human interactions. Here we compare movement, reef-fidelity, and ocean migration for tiger sharks, Galeocerdo cuvier, across the Coral Sea, with an emphasis on New Caledonia. Thirty-three tiger sharks (1.54 to 3.9 m total length) were tagged with passive acoustic transmitters and their localised movements monitored on receiver arrays in New Caledonia, the Chesterfield and Lord Howe Islands in the Coral Sea, and the east coast of Queensland, Australia. Satellite tags were also used to determine habitat use and movements among habitats across the Coral Sea. Sub-adults and one male adult tiger shark displayed year-round residency in the Chesterfields with two females tagged in the Chesterfields and detected on the Great Barrier Reef, Australia, after 591 and 842 days respectively. In coastal barrier reefs, tiger sharks were transient at acoustic arrays and each individual demonstrated a unique pattern of occurrence. From 2009 to 2013, fourteen sharks with satellite and acoustic tags undertook wide-ranging movements up to 1114 km across the Coral Sea with eight detected back on acoustic arrays up to 405 days after being tagged. Tiger sharks dove 1136 m and utilised three-dimensional activity spaces averaged at 2360 km3. The Chesterfield Islands appear to be important habitat for sub-adults and adult male tiger sharks. Management strategies need to consider the wide-ranging movements of large (sub-adult and adult) male and female tiger sharks at the individual level, whereas fidelity to specific coastal reefs may be consistent across groups of individuals. Coastal barrier reef MPAs, however, only afford brief protection for large tiger sharks, therefore determining the importance of other oceanic Coral Sea reefs should be a priority for future research.
    Description: Funding was provided by the the Agence Francaise de Développement (http://www.afd.fr), French Pacific Fund, the CRISP program (www.crisponline.info) and QLD Fisheries.
    Repository Name: Woods Hole Open Access Server
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  • 73
    Publication Date: 2022-05-26
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 10 (2015): e0136376, doi:10.1371/journal.pone.0136376.
    Description: Polar petroleum components enter marine environments through oil spills and natural seepages each year. Lately, they are receiving increased attention due to their potential toxicity to marine organisms and persistence in the environment. We conducted a laboratory experiment and employed state-of-the-art Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) to characterize the polar petroleum components within two operationally-defined seawater fractions: the water-soluble fraction (WSF), which includes only water-soluble molecules, and the water-accommodated fraction (WAF), which includes WSF and microscopic oil droplets. Our results show that compounds with higher heteroatom (N, S, O) to carbon ratios (NSO:C) than the parent oil were selectively partitioned into seawater in both fractions, reflecting the influence of polarity on aqueous solubility. WAF and WSF were compositionally distinct, with unique distributions of compounds across a range of hydrophobicity. These compositional differences will likely result in disparate impacts on environmental health and organismal toxicity, and thus highlight the need to distinguish between these often-interchangeable terminologies in toxicology studies. We use an empirical model to estimate hydrophobicity character for individual molecules within these complex mixtures and provide an estimate of the potential environmental impacts of different crude oil components.
    Description: This study is funded by the Gulf of Mexico Research Initiative (GOMRI) Project # 161684 to Dr. Elizabeth B. Kujawinski.
    Repository Name: Woods Hole Open Access Server
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  • 74
    Publication Date: 2022-05-26
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 10 (2015): e0135381, doi:10.1371/journal.pone.0135381.
    Description: Cephalopods are famous for their ability to change color and pattern rapidly for signaling and camouflage. They have keen eyes and remarkable vision, made possible by photoreceptors in their retinas. External to the eyes, photoreceptors also exist in parolfactory vesicles and some light organs, where they function using a rhodopsin protein that is identical to that expressed in the retina. Furthermore, dermal chromatophore organs contain rhodopsin and other components of phototransduction (including retinochrome, a photoisomerase first found in the retina), suggesting that they are photoreceptive. In this study, we used a modified whole-mount immunohistochemical technique to explore rhodopsin and retinochrome expression in a number of tissues and organs in the longfin squid, Doryteuthis pealeii. We found that fin central muscles, hair cells (epithelial primary sensory neurons), arm axial ganglia, and sucker peduncle nerves all express rhodopsin and retinochrome proteins. Our findings indicate that these animals possess an unexpected diversity of extraocular photoreceptors and suggest that extraocular photoreception using visual opsins and visual phototransduction machinery is far more widespread throughout cephalopod tissues than previously recognized.
    Description: This research was supported by the Office of Naval Research Basic Research Challenge grant number N00014-10-0989 to TWC and RTH and a Biotechnology and Biological Sciences Research Council (BBSRC) David Phillips Fellowship BB/L024667/1 to TJW. The authors gratefully acknowledge support from the Air Force Office of Scientific Research via grants numbered FA9550-09-0346 to RTH. and FA9550-12-1-0321 to TWC.
    Repository Name: Woods Hole Open Access Server
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  • 75
    Publication Date: 2022-05-26
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS 11 (2016): e0149998, doi:10.1371/journal.pone.0149998.
    Description: Individuals with cystic fibrosis (CF) often acquire chronic lung infections that lead to irreversible damage. We sought to examine regional variation in the microbial communities in the lungs of individuals with mild-to-moderate CF lung disease, to examine the relationship between the local microbiota and local damage, and to determine the relationships between microbiota in samples taken directly from the lung and the microbiota in spontaneously expectorated sputum. In this initial study, nine stable, adult CF patients with an FEV1〉50% underwent regional sampling of different lobes of the right lung by bronchoalveolar lavage (BAL) and protected brush (PB) sampling of mucus plugs. Sputum samples were obtained from six of the nine subjects immediately prior to the procedure. Microbial community analysis was performed on DNA extracted from these samples and the extent of damage in each lobe was quantified from a recent CT scan. The extent of damage observed in regions of the right lung did not correlate with specific microbial genera, levels of community diversity or composition, or bacterial genome copies per ml of BAL fluid. In all subjects, BAL fluid from different regions of the lung contained similar microbial communities. In eight out of nine subjects, PB samples from different regions of the lung were also similar in microbial community composition, and were similar to microbial communities in BAL fluid from the same lobe. Microbial communities in PB samples were more diverse than those in BAL samples, suggesting enrichment of some taxa in mucus plugs. To our knowledge, this study is the first to examine the microbiota in different regions of the CF lung in clinically stable individuals with mild-to-moderate CF-related lung disease.
    Description: Support from the Cystic Fibrosis Foundation Research Development Program (STANTO07R0) as a pilot grant to DAH and AA. Research reported in this publication was also supported by grants from the National Institutes of Health to DAH (R01 AI091702 to DAH) and the American Asthma Foundation Scholars Award and CFFT-ASHARE15A0 and R01HL122372 to AA and R01 HL074175 (BAS). The Dartmouth Lung Biology Center and CF Translational Research Core was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P30GM106394 and by the CFF RDP (CFRDP STANTO11R0).
    Repository Name: Woods Hole Open Access Server
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  • 76
    Publication Date: 2022-05-26
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS 11 (2016): e0150660, doi:10.1371/journal.pone.0150660.
    Description: Sperm whales are present in the Canary Islands year-round, suggesting that the archipelago is an important area for this species in the North Atlantic. However, the area experiences one of the highest reported rates of sperm whale ship-strike in the world. Here we investigate if the number of sperm whales found in the archipelago can sustain the current rate of ship-strike mortality. The results of this study may also have implications for offshore areas where concentrations of sperm whales may coincide with high densities of ship traffic, but where ship-strikes may be undocumented. The absolute abundance of sperm whales in an area of 52933 km2, covering the territorial waters of the Canary Islands, was estimated from 2668 km of acoustic line-transect survey using Distance sampling analysis. Data on sperm whale diving and acoustic behaviour, obtained from bio-logging, were used to calculate g(0) = 0.92, this is less than one because of occasional extended periods when whales do not echolocate. This resulted in an absolute abundance estimate of 224 sperm whales (95% log-normal CI 120–418) within the survey area. The recruitment capability of this number of whales, some 2.5 whales per year, is likely to be exceeded by the current ship-strike mortality rate. Furthermore, we found areas of higher whale density within the archipelago, many coincident with those previously described, suggesting that these are important habitats for females and immature animals inhabiting the archipelago. Some of these areas are crossed by active shipping lanes increasing the risk of ship-strikes. Given the philopatry in female sperm whales, replacement of impacted whales might be limited. Therefore, the application of mitigation measures to reduce the ship-strike mortality rate seems essential for the conservation of sperm whales in the Canary Islands.
    Repository Name: Woods Hole Open Access Server
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  • 77
    Publication Date: 2022-05-26
    Description: © The American Chemical Society, 2016. This is an open access article published under an ACS AuthorChoice License. The definitive version was published in Analytical Chemistry 88 (2016): 7154–7162, doi:10.1021/acs.analchem.6b01260.
    Description: Discovery and identification of molecular biomarkers in large LC/MS data sets requires significant automation without loss of accuracy in the compound screening and annotation process. Here, we describe a lipidomics workflow and open-source software package for high-throughput annotation and putative identification of lipid, oxidized lipid, and oxylipin biomarkers in high-mass-accuracy HPLC-MS data. Lipid and oxylipin biomarker screening through adduct hierarchy sequences, or LOBSTAHS, uses orthogonal screening criteria based on adduct ion formation patterns and other properties to identify thousands of compounds while providing the user with a confidence score for each assignment. Assignments are made from one of two customizable databases; the default databases contain 14 068 unique entries. To demonstrate the software’s functionality, we screened more than 340 000 mass spectral features from an experiment in which hydrogen peroxide was used to induce oxidative stress in the marine diatom Phaeodactylum tricornutum. LOBSTAHS putatively identified 1969 unique parent compounds in 21 869 features that survived the multistage screening process. While P. tricornutum maintained more than 92% of its core lipidome under oxidative stress, patterns in biomarker distribution and abundance indicated remodeling was both subtle and pervasive. Treatment with 150 μM H2O2 promoted statistically significant carbon-chain elongation across lipid classes, with the strongest elongation accompanying oxidation in moieties of monogalactosyldiacylglycerol, a lipid typically localized to the chloroplast. Oxidative stress also induced a pronounced reallocation of lipidome peak area to triacylglycerols. LOBSTAHS can be used with environmental or experimental data from a variety of systems and is freely available at https://github.com/vanmooylipidomics/LOBSTAHS.
    Description: This research was supported by the Gordon and Betty Moore Foundation through Grant GBMF3301 to B.A.S.V.M. This research was also funded in part by a grant to B.A.S.V.M. from the Simons Foundation and is a contribution of the Simons Collaboration on Ocean Processes and Ecology (SCOPE). J.R.C. acknowledges support from a U.S. Environmental Protection Agency (EPA) STAR Graduate Fellowship (Fellowship Assistance Agreement No. FP-91744301-0).
    Repository Name: Woods Hole Open Access Server
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  • 78
    Publication Date: 2022-05-26
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS 11 (2016): e0151089, doi: 10.1371/journal.pone.0151089 .
    Description: The majority of ovarian tumors eventually recur in a drug resistant form. Using cisplatin sensitive and resistant cell lines assembled into 3D spheroids we profiled gene expression and identified candidate mechanisms and biological pathways associated with cisplatin resistance. OVCAR-8 human ovarian carcinoma cells were exposed to sub-lethal concentrations of cisplatin to create a matched cisplatin-resistant cell line, OVCAR-8R. Genome-wide gene expression profiling of sensitive and resistant ovarian cancer spheroids identified 3,331 significantly differentially expressed probesets coding for 3,139 distinct protein-coding genes (Fc 〉2, FDR 〈 0.05) (S2 Table). Despite significant expression changes in some transporters including MDR1, cisplatin resistance was not associated with differences in intracellular cisplatin concentration. Cisplatin resistant cells were significantly enriched for a mesenchymal gene expression signature. OVCAR-8R resistance derived gene sets were significantly more biased to patients with shorter survival. From the most differentially expressed genes, we derived a 17-gene expression signature that identifies ovarian cancer patients with shorter overall survival in three independent datasets. We propose that the use of cisplatin resistant cell lines in 3D spheroid models is a viable approach to gain insight into resistance mechanisms relevant to ovarian tumors in patients. Our data support the emerging concept that ovarian cancers can acquire drug resistance through an epithelial-to-mesenchymal transition.
    Description: This work was funded by the NIH NCRR supplement grant P41 RR001395-27S1 (J.W.H.), NSF DBI-1005378 “REU Site: Biological Discovery in Woods Hole”, faculty startup funds from the Office of Research at Oklahoma State University (W.C.), and the Mary Kay Foundation (A.S.B.).
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  • 79
    Publication Date: 2023-08-29
    Description: The sedimentary dynamics and geological evolution of the Naples canyons during the Late Quaternary have been studied based on sedimentological and seismo-stratigraphic data. Several factors, including the sedimentary environments, tectonic setting, and volcanic eruptions, have controlled the geological evolution of the coastal and marine areas of the Bay of Naples. The main data and methods include the sedimentological data analysis, the seismo-stratigraphic techniques applied in the geological interpretation of seismic profiles, and the integrated analysis of core data that were previously published. The formation of the Dohrn canyon is controlled by fluvial processes, active in correspondence with the palaeo-Schiazzano River system and by the main eruptive events involving the submarine portion of Naples Bay, including the Campanian Ignimbrite (CI; 39 ky B.P.) and the Neapolitan Yellow Tuff (NYT; 15 ky B.P.). The formation of the Magnaghi canyon is controlled by erosional processes on the continental slope of Procida Island, which was active during the last eruptive phases of the island (Solchiaro Formation; 18 ky B.P.), triggering high rates of volcaniclastic supply.
    Description: Published
    Description: 226
    Description: 1T. Struttura della Terra
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 80
    Publication Date: 2023-08-29
    Description: The chemical composition of rainwater was studied in two highly-industrialised areas in Sicily (southern Italy), between June 2018 and July 2019. The study areas were characterised by large oil refining plants and other industrial hubs whose processes contribute to the release of large amounts of gaseous species that can affect the chemical composition of atmospheric deposition As in most of the Mediterranean area, rainwater acidity (ranging in the study area between 3.9 and 8.3) was buffered by the dissolution of abundant geogenic carbonate aerosol. In particular, calcium and magnesium cations showed the highest pH-neutralizing factor, with ~92% of the acidity brought by SO42- and NO3- neutralized by alkaline dust. The lowest pH values were observed in samples collected after abundant rain periods, characterised by a less significant dry deposition of alkaline materials. Electrical Conductivity (ranging between 7 µS cm-1 and 396 µS cm-1) was inversely correlated with the amount of rainfall measured in the two areas. Concentrations of major ionic species followed the sequence Cl- 〉 Na+ 〉 SO42- ≃ HCO3- 〉 ≃ Ca2+ 〉 NO3- 〉 Mg2+ 〉 K+ 〉 F-. High loads of Na+ and Cl- (with a calculated R2 = 0.99) reflected proximity to the sea. Calcium, potassium, and non-sea-salt magnesium had a prevalent crustal origin. Non-sea salt sulphate, nitrate, and fluoride can be attributed mainly to anthropogenic sources. Mt. Etna, during eruptive periods, may be also considered, on a regional scale, a significant source for fluoride, non-sea salt sulphate, and even chloride.
    Description: Published
    Description: 3898
    Description: 6A. Geochimica per l'ambiente e geologia medica
    Description: JCR Journal
    Keywords: acidity neutralization ; anthropogenic source ; atmospheric deposition ; major ions ; marine source ; 01.01. Atmosphere
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 81
    Publication Date: 2023-09-06
    Description: Lagrangian dispersion of fluid particle pairs refers to the study of how individual fluid particles disperse and move in a fluid flow, providing insights to understand transport phenomena in various environments, from laminar to turbulent conditions. Here, we explore this phenomenon in synthetic velocity and magnetic fields generated through a reduced-order model of the magnetohy- drodynamic equations, which is able to mimic both a laminar and a turbulent environment. In the case of laminar conditions, we find that the average square distance between particle pairs increases linearly with time, implying a dispersion pattern similar to Brownian motion at all time steps. On the other hand, under turbulent conditions, surprisingly enough we observe a Richardson scaling, indicating a super-ballistic dispersion pattern, which aligns with the expected scaling properties for a turbulent environment. Additionally, our study reveals that the magnetic field plays an organizing role. Lastly, we explore a purely hydrodynamic case without magnetic field effects, showing that, even in a turbulent environment, the behavior remains Brownian-like, highlighting the crucial role of the magnetic field in generating the Richardson scaling observed in our model.
    Description: Published
    Description: 662
    Description: 2A. Fisica dell'alta atmosfera
    Description: JCR Journal
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  • 82
    Publication Date: 2023-09-07
    Description: The La Fossa volcano is near the inhabited zone of the island of Vulcano and is a suitable case for studying gas sources of different geological origins. Since the last eruption, fumarolic-solfataric ac-tivity has interested this area with fumarolic emissions, mainly at the top of the volcanic cone and at Vulcano Porto. In recent decades, the anomalous degassing zones on the island have not significantly changed their location. On the contrary, there have been several significant changes in the emission rate due to the addition of volcanic gas. In these zones, CO2 flux from the ground is responsible for a decrease in the indoor air quality. A recent increase in volcanic degassing led to an increase in the gas hazard in the inhabited area of Vulcano Island, and people were temporarily displaced from Vulcano Porto. The results of this study show that a monitoring system can be used for the early detection of transients in soil CO2 flux (φCO2) in the anomalous degassing zone of Vulcano. Syn-chronous monitoring of φCO2 and outdoor air CO2 concentration has shown variations in volcanic degassing that affect outdoor air CO2 concentration in the populated zone of Faraglione.
    Description: This research was funded by Dipartimento di Protezione Civile—Convenzione DPC-INGV 2019–2021 All.B2, WP Vulcani, Task 16; Scientific Advisor and Project Manager: Sergio Gurrieri.
    Description: Published
    Description: 266
    Description: 6V. Pericolosità vulcanica e contributi alla stima del rischio
    Description: 1TR. Georisorse
    Description: 6SR VULCANI – Servizi e ricerca per la società
    Description: 1IT. Reti di monitoraggio e sorveglianza
    Description: JCR Journal
    Keywords: soil CO2 flux ; volcano monitoring ; volcanic unrest ; gas hazard ; air CO2 concentration ; volcanic hazard ; geochemistry ; diffuse degassing ; Vulcano ; Aeolian islands ; geochemical monitoring networdk ; indoor CO2
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 83
    Publication Date: 2023-09-11
    Description: Volcano monitoring is the key approach in mitigating the risks associated with volcanic phenomena. Although Antarctic volcanoes are characterized by remoteness, the 2010 Eyjafjallajökull eruption and the 2022 Hunga eruption have reminded us that even the farthest and/or least-known volcanoes can pose significant hazards to large and distant communities. Hence, it is important to also develop monitoring systems in the Antarctic volcanoes, which involves installing and maintaining multiparametric instrument networks. These tasks are particularly challenging in polar regions as the instruments have to face the most extreme climate on the Earth, characterized by very low temperatures and strong winds. In this work, we describe the multiparametric monitoring system recently deployed on the Melbourne volcano (Victoria Land, Antarctica), consisting of seismic, geochemical and thermal sensors together with powering, transmission and acquisition systems. Particular strategies have been applied to make the monitoring stations efficient despite the extreme weather conditions. Fumarolic ice caves, located on the summit area of the Melbourne volcano, were chosen as installation sites as they are protected places where no storm can damage the instruments and temperatures are close to 0 °C all year round. In addition, the choice of instruments and their operating mode has also been driven by the necessity to reduce energy consumption. Indeed, one of the most complicated tasks in Antarctica is powering a remote instrument year-round. The technological solutions found to implement the monitoring system of the Melbourne volcano and described in this work can help create volcano monitoring infrastructures in other polar environments.
    Description: Published
    Description: 7594
    Description: 6V. Pericolosità vulcanica e contributi alla stima del rischio
    Description: JCR Journal
    Keywords: Antarctica ; extreme weather conditions ; sensor network ; volcano monitoring
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  • 84
    Publication Date: 2023-10-12
    Description: The exacerbation of wildfires, attributed to the effects of climate change, presents substantial risks to ecological systems, infrastructure, and human well-being. In the context of the Sustainable Development Goals (SDGs), particularly those related to climate action, prioritizing the assessment and management of the occurrence and intensity of extensive wildfires is of utmost importance. In recent times, there has been a significant increase in the frequency and severity of widespread wildfires worldwide, affecting several locations, including Australia, Italy, and the United States of America. The presence of complex phenomena marked by limited predictability leads to significant negative impacts on biodiversity and human lives. The utilization of satellite-derived data with neural networks, such as convolutional neural networks (CNNs), is a potentially advantageous approach for augmenting the monitoring capabilities of wildfires. This research examines the generalization capability of four neural network models, namely the fully connected (FC), one-dimensional (1D) CNN, two-dimensional (2D) CNN, and three-dimensional (3D) CNN model. Each model’s performance, as measured by accuracy, recall, and F1 scores, is assessed through K-fold cross-validation. Subsequently, T-statistics and p-values are computed based on these metrics to conduct a statistical comparison among the different models, allowing us to quantify the degree of similarity or dissimilarity between them. By using training data from Australia and Sicily, the performances of the trained model are evaluated on the test dataset from Oregon. The results are promising, with cross-validation on the training dataset producing mean precision, recall, and F1 scores ranging between approximately 0.97 and 0.98. Especially, the fully connected model has superior generalization capabilities, whilst the 3D CNN offers more refined and less distorted classifications. However, certain issues, such as false fire detection and confusion between smoke and shadows, persist. The aforementioned methodologies offer significant perspectives on the capabilities of neural network technologies in supporting the detection and management of wildfires. These approaches address the crucial matter of domain transferability and the associated dependability of predictions in new regions. This study makes a valuable contribution to the ongoing efforts in climate change by assisting in monitoring and managing wildfires.
    Description: Published
    Description: 4855
    Description: 5IT. Osservazioni satellitari
    Description: JCR Journal
    Keywords: wildfire ; PRISMA ; Convolutional Neural Networks ; hyperspectral
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 85
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    Unknown
    American Geophysical Union (AGU)
    In:  EPIC3Geophysical Research Letters, American Geophysical Union (AGU), 50(17), ISSN: 0094-8276
    Publication Date: 2023-09-08
    Description: We quantify sea ice concentration (SIC) changes related to synoptic cyclones separately for each month of the year in the Greenland, Barents and Kara Seas for 1979–2018. We find that these SIC changes can be statistically significant throughout the year. However, their strength varies from region to region and month to month, and their sign strongly depends on the considered time scale (before/during vs. after cyclone passages). Our results show that the annual cycle of cyclone impacts on SIC is related to varying cyclone intensity and traversed sea ice conditions. We further show that significant changes in these cyclone impacts have manifested in the last 40 years, with the strongest changes occurring in October and November. For these months, SIC decreases before/during cyclones have more than doubled in magnitude in the Barents and Kara Seas, while SIC increases following cyclones have weakened (intensified) in the Barents Sea (Kara Sea).
    Repository Name: EPIC Alfred Wegener Institut
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  • 86
    Publication Date: 2023-09-15
    Description: 〈jats:p〉Regional climate models are a valuable tool for the study of the climate processes and climate change in polar regions, but the performance of the models has to be evaluated using experimental data. The regional climate model CCLM was used for simulations for the MOSAiC period with a horizontal resolution of 14 km (whole Arctic). CCLM was used in a forecast mode (nested in ERA5) and used a thermodynamic sea ice model. Sea ice concentration was taken from AMSR2 data (C15 run) and from a high-resolution data set (1 km) derived from MODIS data (C15MOD0 run). The model was evaluated using radiosonde data and data of different profiling systems with a focus on the winter period (November–April). The comparison with radiosonde data showed very good agreement for temperature, humidity, and wind. A cold bias was present in the ABL for November and December, which was smaller for the C15MOD0 run. In contrast, there was a warm bias for lower levels in March and April, which was smaller for the C15 run. The effects of different sea ice parameterizations were limited to heights below 300 m. High-resolution lidar and radar wind profiles as well as temperature and integrated water vapor (IWV) data from microwave radiometers were used for the comparison with CCLM for case studies, which included low-level jets. LIDAR wind profiles have many gaps, but represent a valuable data set for model evaluation. Comparisons with IWV and temperature data of microwave radiometers show very good agreement.〈/jats:p〉
    Repository Name: EPIC Alfred Wegener Institut
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  • 87
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    American Geophysical Union (AGU)
    In:  EPIC3Geophysical Research Letters, American Geophysical Union (AGU), 49(23), ISSN: 0094-8276
    Publication Date: 2023-09-19
    Description: The strong cooling during the Last Glacial Maximum (LGM, 21 ka BP) provides a rigorous test of climate models' ability to simulate past and future climate changes. We force an atmospheric general circulation model with two recent global LGM sea surface temperature (SST) reconstructions, one suggesting a weak and the other a more pronounced cooling, and compare the simulated land surface temperatures (LSTs) to reconstructed data. Our results do not confirm either SST reconstruction. The cold SST data set leads to good agreement between simulated and observed LSTs at low latitudes, but is systematically too cold at mid-latitudes. The opposite is true for the warm SST data set. Differences between the simulated LSTs are caused by varying land surface albedos, which is lower for the warmer SST reconstruction. The inconsistency between reconstructed and simulated climate points to a potentially significant bias in the proxy reconstructions and/or the climate sensitivity of current climate models.
    Repository Name: EPIC Alfred Wegener Institut
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  • 88
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    American Geophysical Union (AGU)
    In:  EPIC3Reviews of Geophysics, American Geophysical Union (AGU), 61(3), ISSN: 8755-1209
    Publication Date: 2023-10-09
    Description: Knowledge of Antarctica's sedimentary basins builds our understanding of the coupled evolution of tectonics, ice, ocean, and climate. Sedimentary basins have properties distinct from basement-dominated regions that impact ice-sheet dynamics, potentially influencing future ice-sheet change. Despite their importance, our knowledge of Antarctic sedimentary basins is restricted. Remoteness, the harsh environment, the overlying ice sheet, ice shelves, and sea ice all make fieldwork challenging. Nonetheless, in the past decade the geophysics community has made great progress in internationally coordinated data collection and compilation with parallel advances in data processing and analysis supporting a new insight into Antarctica's subglacial environment. Here, we summarize recent progress in understanding Antarctica's sedimentary basins. We review advances in the technical capability of radar, potential fields, seismic, and electromagnetic techniques to detect and characterize basins beneath ice and advances in integrated multi-data interpretation including machine-learning approaches. These new capabilities permit a continent-wide mapping of Antarctica's sedimentary basins and their characteristics, aiding definition of the tectonic development of the continent. Crucially, Antarctica's sedimentary basins interact with the overlying ice sheet through dynamic feedbacks that have the potential to contribute to rapid ice-sheet change. Looking ahead, future research directions include techniques to increase data coverage within logistical constraints, and resolving major knowledge gaps, including insufficient sampling of the ice-sheet bed and poor definition of subglacial basin structure and stratigraphy. Translating the knowledge of sedimentary basin processes into ice-sheet modeling studies is critical to underpin better capacity to predict future change.
    Repository Name: EPIC Alfred Wegener Institut
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  • 89
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    American Geophysical Union (AGU)
    In:  EPIC3Geophysical Research Letters, American Geophysical Union (AGU), 50(22), ISSN: 0094-8276
    Publication Date: 2023-11-25
    Description: Given the role played by the historical and extensive coverage of sea ice concentration (SIC) observations in reconstructing the long‐term variability of Antarctic sea ice, and the limited attention given to model‐dependent parameters in current sea ice data assimilation studies, this study focuses on enhancing the performance of the Data Assimilation System for the Southern Ocean in assimilating SIC through optimizing the localization and observation error estimate, and two assimilation experiments were conducted from 1979 to 2018. By comparing the results with the sea ice extent of the Southern Ocean and the sea ice thickness in the Weddell Sea, it becomes evident that the experiment with optimizations outperforms that without optimizations due to achieving more reasonable error estimates. Investigating uncertainties of the sea ice volume anomaly modeling reveals the importance of the sea ice‐ocean interaction in the SIC assimilation, implying the necessity of assimilating more oceanic and sea‐ice observations.
    Repository Name: EPIC Alfred Wegener Institut
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  • 90
    Publication Date: 2023-10-31
    Description: Among the effects of space weather, the degradation of air traffic communications and satellite-based navigation systems are the most notable. For this reason, it is of uttermost importance to understand the nature and origin of ionospheric irregularities that are at the base of the observed communication outages. Here we focus on polar cap patches (PCPs) that constitute a special class of ionospheric irregularities observed at very high latitudes in the F region. To this purpose we use the so-called PCP flag, a Swarm Level 2 product, that allows for identifying PCPs. We relate the presence of PCPs to the values of the first- and second-order scaling exponents and intermittency estimated from Swarm A electron density fluctuations and to the values of the Rate Of change of electron Density Index (RODI) for two different levels of geomagnetic activity, over a time span of approximately 3.5 years starting on 16 July 2014. Our findings show that values of RODI, first- and second-order scaling exponents and intermittency corresponding to measurements taken inside PCPs differ from those corresponding to measurements taken outside PCPs. Additionally, the values of the first- and second-order scaling exponents and of intermittency indicate that PCPs are in a turbulent state. Investigation of the coincidence of loss of lock (LoL) events with PCPs displayed that approximately 57.4% of LoLs in the Northern hemisphere and 45.7% in the Southern hemisphere occur in coincidence of PCPs when disturbed geomagnetic activity is considered. During quiet geomagnetic conditions these percentages decrease to 51.4% in the Northern hemisphere and to 20.1% in the Southern hemisphere.
    Description: Published
    Description: 4320
    Description: OSA3: Climatologia e meteorologia spaziale
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 91
    Publication Date: 2023-10-27
    Description: This paper describes the main updates characterizing the new version of IONORT (IONOsperic Ray Tracing), a software tool developed at Istituto Nazionale di Geofisica e Vulcanologia to determine both the path of a high frequency (HF) radio wave propagating in the ionospheric medium, and the group time delay of the wave itself along the path. One of the main changes concerns the replacement of a regional three-dimensional electron density matrix, which was previously taken as input to represent the ionosphere, with a global one. Therefore, it is now possible to carry out different ray tracings from whatever point of the Earth’s surface, simply by selecting suitable loop cycles thanks to the new ray tracing graphical user interface (GUI). At the same time, thanks to a homing GUI, it is also possible to generate synthetic oblique ionograms for whatever radio link chosen by the user. Both ray tracing and homing GUIs will be described in detail providing at the same time some practical examples of their use for different regions. IONORT software finds practical application in the planning of HF radio links, exploiting the sky wave, through an accurate and thorough knowledge of the ionospheric medium. HF radio waves users, including broadcasting and civil aviation, would benefit from the use of the IONORT software (version 2023.10).
    Description: Published
    Description: 5111
    Description: OSA3: Climatologia e meteorologia spaziale
    Description: JCR Journal
    Keywords: ionospheric HF ray tracing ; homing ; three-dimensional electron density ; iRI model ; oblique sounding ; space weather ; 01.02. Ionosphere
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 92
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    American Geophysical Union (AGU)
    In:  EPIC3Geophysical Research Letters, American Geophysical Union (AGU), 50(21), ISSN: 0094-8276
    Publication Date: 2023-11-01
    Description: Molybdenum (Mo) is a trace element sensitive to oceanic redox conditions. The fidelity of sedimentary Mo as a paleoredox proxy of coeval seawater depends on the extent of Mo remobilization during postdepositional processes. Here we present the Mo content and isotope profiles for deep sediments from the Nankai Trough, Japan. The Mo signature suggests that these sediments have experienced extensive early diagenesis and hydrothermal alteration at depth. Iron (Fe)‐manganese (Mn) (oxyhydr)oxide alteration combined with Mo thiolation leads to a more than twenty‐fold enrichment of Mo within the sulfate reduction zone. Hydrothermal fluids and Mo adsorption onto Fe‐Mn (oxyhydr)oxides cause extremely negative Mo‐isotope values at the underthrust zone. These postdepositional Mo signals might be misinterpreted as expanded anoxia in the water column. Our findings highlight the importance of constraining postdepositional effects on Mo‐based proxies during paleoredox reconstruction.
    Repository Name: EPIC Alfred Wegener Institut
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  • 93
    Publication Date: 2023-12-19
    Description: As a contribution to the Regional Carbon Cycle Assessment and Processes phase 2 (RECCAP2) project, we present synthesized estimates of Arctic Ocean sea-air CO2 fluxes and their uncertainties from surface ocean pCO2-observation products, ocean biogeochemical hindcast and data assimilation models, and atmospheric inversions. For the period of 1985–2018, the Arctic Ocean was a net sink of CO2 of 116 ± 4 TgC yr−1 in the pCO2 products, 92 ± 30 TgC yr−1 in the models, and 91 ± 21 TgC yr−1 in the atmospheric inversions. The CO2 uptake peaks in late summer and early autumn, and is low in winter when sea ice inhibits sea-air fluxes. The long-term mean CO2 uptake in the Arctic Ocean is primarily caused by steady-state fluxes of natural carbon (70% ± 15%), and enhanced by the atmospheric CO2 increase (19% ± 5%) and climate change (11% ± 18%). The annual mean CO2 uptake increased from 1985 to 2018 at a rate of 31 ± 13 TgC yr−1 dec−1 in the pCO2 products, 10 ± 4 TgC yr−1 dec−1 in the models, and 32 ± 16 TgC yr−1 dec−1 in the atmospheric inversions. Moreover, 77% ± 38% of the trend in the net CO2 uptake over time is caused by climate change, primarily due to rapid sea ice loss in recent years. Furthermore, true uncertainties may be larger than the given ensemble standard deviations due to common structural biases across all individual estimates.
    Repository Name: EPIC Alfred Wegener Institut
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  • 94
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    American Association for the Advancement of Science (AAAS)
    In:  EPIC3Science Advances, American Association for the Advancement of Science (AAAS), 9(50), ISSN: 2375-2548
    Publication Date: 2023-12-18
    Description: Antarctic krill, crucial to the Southern Ocean ecosystem and a vital fisheries resource, is endangered by climate change. Identifying drivers of krill biomass is therefore essential for determining catch limits and designating protection zones. We present a modeling approach to pinpointing effects of sea surface temperature, ice cover, chlorophyll levels, climate indices, and intraspecific competition. Our study reveals that larval recruitment is driven by both competition among age classes and chlorophyll levels. In addition, while milder ice and temperature in spring and summer favor reproduction and early larval survival, both larvae and juveniles strongly benefit from heavier ice and colder temperatures in winter. We conclude that omitting top-down control of resources by krill is only acceptable for retrospective or single-year prognostic models that use field chlorophyll data but that incorporating intraspecific competition is essential for longer-term forecasts. Our findings can guide future krill modeling strategies, reinforcing the sustainability of this keystone species.
    Repository Name: EPIC Alfred Wegener Institut
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  • 95
    Publication Date: 2023-12-07
    Description: Natural hazards are increasingly threatening our communities; hence it is imperative to provide communities with reliable information on possible impacts of such disasters, and on resilience measures that can be adopted to recover from disasters. To increase the engagement of various stakeholders in decision-making processes related to resilience to natural hazards, problem-specific information needs to be presented to them in a language understandable to non-experts in the field. To this end, this paper illustrates experimentation with low-code platforms for fast digitalization of resilience reports, incorporating the perspectives of various stakeholders in the analysis, thus making informed decision-making practicable. We present a co-creation-based approach to develop GIS-based user-friendly dashboards in support to the identification of resilience strategies against natural hazards; this approach has been developed within the framework of the European project ARCH. Urban areas are regarded as complex social-ecological systems whose various dimensions should be considered in this resilience endeavor, during all phases of the Disaster Risk Reduction and Climate Change Adaptation cycle. The work presented in this paper specifically targets the possible impacts and risks that might affect the cultural heritage subsystems of our cities, generally underrepresented in the international literature related to urban resilience assessment. We describe how we applied our approach to the Camerino municipality, a historic Italian town exposed to seismic risk, which was struck by a severe earthquake sequence in 2016–2017 and discuss the results of our experience.
    Description: The research activities have been funded by the project “ARCH—Advancing Resilience of historic areas against Climate-related and other Hazards” funded by Horizon 2020—European Union Research and Innovation Program under grant agreement No. 820999. The sole responsibility for the content of this publication lies with the authors. It does not necessarily represent the opinion of the European Union. Neither the EASME, nor REA, nor the European Commission is responsible for any use that may be made of the information contained therein. The work is also funded by Project 1.7 “Technologies for the efficient penetration of the electric vector in the final uses” within the “Electrical System Research” PTR 22–24.
    Description: Published
    Description: 65
    Description: OST4 Descrizione in tempo reale del terremoto, del maremoto, loro predicibilità e impatto
    Description: JCR Journal
    Keywords: urban resilience ; cultural heritage ; geographic information system ; seismic risk ; knowledge representation ; climate change ; 05.08. Risk ; 05.06. Methods
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 96
    Publication Date: 2023-11-20
    Description: In this study, we focus on the eruption of Mount Etna on Christmas 2018, which emitted great amounts of SO2 from 24th to 30th December into the free troposphere. Simulations based on two different estimations of SO2 emission fluxes are conducted with the chemistry-transport model MOCAGE in order to study the impact of these estimations on the volcanic plume modeling. The two flux emissions used are retrieved (1) from the ground-based network FLAME, located on the flank of the volcano, and (2) from the spaceborne instrument SEVIRI onboard the geostationary satellite MSG. Multiple spaceborne observations, in the infrared and ultraviolet bands, are used to evaluate the model results. Overall, the model results match well with the plume location over the period of the eruption showing the good transport of the volcanic plume by the model, which is linked to the use of a realistic estimation of the altitude of injection of the emissions. However, there are some discrepancies in the plume concentrations of SO2 between the two simulations, which are due to the differences between the two emission flux estimations used that are large on some of the days. These differences are linked to uncertainties in the retrieval methods and observations used to derive SO2 volcanic fluxes. We find that the uncertainties in the satellite-retrieved column of SO2 used for the evaluation of the simulations, linked to the instrument sensitivity and/or the retrieval algorithm, are sometimes nearly as large as the differences between the two simulations. This shows a limitation of the use of satellite retrievals of SO2 concentrations to quantitatively validate modeled volcanic plumes. In the paper, we also discuss approaches to improve the simulation of SO2 concentrations in volcanic plumes through model improvements and also via more advanced methods to more effectively use satellite-derived products.
    Description: Published
    Description: 758
    Description: 5V. Processi eruttivi e post-eruttivi
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 97
    Publication Date: 2023-12-21
    Description: Here we investigate the suitability of Robinia pseudoacacia L. (black locust) leaflets as a novel biomonitor of airborne microplastics (MPs) including tyre wear particles (TWPs). Leaflets were collected from rural roadside locations (ROs, n = 5) and urban parks (UPs, n = 5) in Siena, Italy. MPs were removed by washing, identified by stereomicroscope, and analysed for polymer type by Fourier transform infrared spectroscopy. Daily MP deposition was estimated from leaf area. The mass magnetic susceptibility and the bioaccumulation of traffic-related potentially toxic elements (PTEs) were also analysed. The total number of MPs at ROs was significantly higher at 2962, dominated by TWPs, compared with 193 in UPs, where TWPs were not found. In contrast, total microfibres were significantly higher in UPs compared with ROs (185 vs. 86). Daily MP deposition was estimated to range from 4.2 to 5.1 MPs/m2/d across UPs and 29.9–457.6 MPs/m2/d across ROs. The polymer types at ROs were dominated by rubber (80%) from TWPs, followed by 15% polyamide (PA) and 5% polysulfone (PES), while in UPs the proportion of PES (44%) was higher than PA (22%) and polyacrylonitrile (11%). The mean mass magnetic susceptibility, a proxy of the bioaccumulation of traffic-related metallic particles, was higher at ROs (0.62 ± 0.01 10–8 m3/kg) than at UPs (–0.50 ± 0.03 10–8 m3/kg). The content of PTEs was similar across sites, except for significantly higher concentrations of Sb, a tracer of vehicle brake wear, at ROs (0.308 ± 0.008 µg/g) compared with UPs (0.054 ± 0.006 µg/g). Our results suggest that the waxy leaflets and easy determination of surface area make Robinia an effective biomonitor for airborne MPs including TWPs.
    Description: this work was partially supported by the INGV project Pianeta Dinamico CHIOMA (CUP D53J19000170001) funded by MUR (Ministry of University and Research).
    Description: Published
    Description: 1456
    Description: OSA1: Variazioni del campo magnetico terrestre, imaging crostale e sicurezza del territorio
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 98
    Publication Date: 2023-12-20
    Description: We assess the Southern Ocean CO2 uptake (1985–2018) using data sets gathered in the REgional Carbon Cycle Assessment and Processes Project Phase 2. The Southern Ocean acted as a sink for CO2 with close agreement between simulation results from global ocean biogeochemistry models (GOBMs, 0.75 ± 0.28 PgC yr−1) and pCO2-observation-based products (0.73 ± 0.07 PgC yr−1). This sink is only half that reported by RECCAP1 for the same region and timeframe. The present-day net uptake is to first order a response to rising atmospheric CO2, driving large amounts of anthropogenic CO2 (Cant) into the ocean, thereby overcompensating the loss of natural CO2 to the atmosphere. An apparent knowledge gap is the increase of the sink since 2000, with pCO2-products suggesting a growth that is more than twice as strong and uncertain as that of GOBMs (0.26 ± 0.06 and 0.11 ± 0.03 Pg C yr−1 decade−1, respectively). This is despite nearly identical pCO2 trends in GOBMs and pCO2-products when both products are compared only at the locations where pCO2 was measured. Seasonal analyses revealed agreement in driving processes in winter with uncertainty in the magnitude of outgassing, whereas discrepancies are more fundamental in summer, when GOBMs exhibit difficulties in simulating the effects of the non-thermal processes of biology and mixing/circulation. Ocean interior accumulation of Cant points to an underestimate of Cant uptake and storage in GOBMs. Future work needs to link surface fluxes and interior ocean transport, build long overdue systematic observation networks and push toward better process understanding of drivers of the carbon cycle.
    Repository Name: EPIC Alfred Wegener Institut
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  • 99
    Publication Date: 2024-01-06
    Description: The seasonal cycle is the dominant mode of variability in the air-sea CO2 flux in most regions of the global ocean, yet discrepancies between different seasonality estimates are rather large. As part of the Regional Carbon Cycle Assessment and Processes Phase 2 project (RECCAP2), we synthesize surface ocean pCO2 and air-sea CO2 flux seasonality from models and observation-based estimates, focusing on both a present-day climatology and decadal changes between the 1980s and 2010s. Four main findings emerge: First, global ocean biogeochemistry models (GOBMs) and observation-based estimates (pCO2 products) of surface pCO2 seasonality disagree in amplitude and phase, primarily due to discrepancies in the seasonal variability in surface DIC. Second, the seasonal cycle in pCO2 has increased in amplitude over the last three decades in both pCO2 products and GOBMs. Third, decadal increases in pCO2 seasonal cycle amplitudes in subtropical biomes for both pCO2 products and GOBMs are driven by increasing DIC concentrations stemming from the uptake of anthropogenic CO2 (Cant). In subpolar and Southern Ocean biomes, however, the seasonality change for GOBMs is dominated by Cant invasion, whereas for pCO2 products an indeterminate combination of Cant invasion and climate change modulates the changes. Fourth, biome-aggregated decadal changes in the amplitude of pCO2 seasonal variability are largely detectable against both mapping uncertainty (reducible) and natural variability uncertainty (irreducible), but not at the gridpoint scale over much of the northern subpolar oceans and over the Southern Ocean, underscoring the importance of sustained high-quality seasonally resolved measurements over these regions.
    Repository Name: EPIC Alfred Wegener Institut
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  • 100
    Publication Date: 2024-01-06
    Description: This contribution to the RECCAP2 (REgional Carbon Cycle Assessment and Processes) assessment analyzes the processes that determine the global ocean carbon sink, and its trends and variability over the period 1985–2018, using a combination of models and observation-based products. The mean sea-air CO2 flux from 1985 to 2018 is −1.6 ± 0.2 PgC yr−1 based on an ensemble of reconstructions of the history of sea surface pCO2 (pCO2 products). Models indicate that the dominant component of this flux is the net oceanic uptake of anthropogenic CO2, which is estimated at −2.1 ± 0.3 PgC yr−1 by an ensemble of ocean biogeochemical models, and −2.4 ± 0.1 PgC yr−1 by two ocean circulation inverse models. The ocean also degasses about 0.65 ± 0.3 PgC yr−1 of terrestrially derived CO2, but this process is not fully resolved by any of the models used here. From 2001 to 2018, the pCO2 products reconstruct a trend in the ocean carbon sink of −0.61 ± 0.12 PgC yr−1 decade−1, while biogeochemical models and inverse models diagnose an anthropogenic CO2-driven trend of −0.34 ± 0.06 and −0.41 ± 0.03 PgC yr−1 decade−1, respectively. This implies a climate-forced acceleration of the ocean carbon sink in recent decades, but there are still large uncertainties on the magnitude and cause of this trend. The interannual to decadal variability of the global carbon sink is mainly driven by climate variability, with the climate-driven variability exceeding the CO2-forced variability by 2–3 times. These results suggest that anthropogenic CO2 dominates the ocean CO2 sink, while climate-driven variability is potentially large but highly uncertain and not consistently captured across different methods.
    Repository Name: EPIC Alfred Wegener Institut
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