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  • 1
    Call number: ZSP-180-Z9
    In: Berichte aus dem Zentrum für Meeres- und Klimaforschung
    Type of Medium: Monograph available for loan
    Pages: 111 S. : graph. Darst.
    ISSN: 0947-7179
    Series Statement: Berichte aus dem Zentrum für Meeres- und Klimaforschung : Reihe Z, Interdisziplinäre Zentrumsberichte 9
    Language: English
    Location: AWI Reading room
    Branch Library: AWI Library
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  • 2
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    PANGAEA
    In:  Supplement to: Zurhelle, Christian; Nieva, Joyce; Tillmann, Urban; Krock, Bernd; Tebben, Jan (2018): Identification of novel gmnodimines and spirolides from the marine dinoflagellate Alexandrium ostenfeldii. 16(11), 446, https://doi.org/10.3390/md16110446
    Publication Date: 2023-03-16
    Description: The strain of Alexandrium ostenfeldii was isolated from a single cell from Ouwerkerkse Kreek.
    Keywords: AWI_EcolChem; BIO; Biology; Ecological Chemistry @ AWI; OuwerkerkseKreek
    Type: Dataset
    Format: application/zip, 386.9 MBytes
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  • 3
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    In:  Supplement to: Ma, Haiyan; Krock, Bernd; Tillmann, Urban; Cembella, Allan (2010): Towards characterization of lytic compound(s) produced by Alexandrium tamarense. Proceedings of the 13th International Conference on Harmful Algae, Ed. Ho, K. et al., International Society for the Study of Harmful Algae and Intergovernmental Oceanographic Commission of UNESCO, Hong Kong, 2008, China, 142-146, hdl:10013/epic.36232.d001
    Publication Date: 2023-01-13
    Description: We investigated optimal conditions for characterization of bioactivity of lytic compound(s) excreted by Alexandrium tamarense based on a cell-bioassay system. Allelochemical response of the cryptophyte Rhodomonas salina indicated the presence oflytic compound(s) in a reliable and reproducible way and allows for quantification of this lytic effect. The parameters tested were the incubation time of putatively lytic extracts or fractions with the target organism R. salina, different techniques for cell harvest from A. tamarense cultures and the optimal harvest time. A three hour incubation time was found to be optimal to yield a rapid response while accurately estimating effective concentration (ECso) values. Harvest of A. tamarense cultures by filtration resulted in loss of lytic activity in most cases and centrifugation was most efficient in terms of recovery of lytic activity. Maximum yield of extracellular lytic activity of A. tamarense cultures was achieved in the stationary phase. Such optimized bioassay guided fractionation techniques are a valuable asset in the isolation and eventual stmctural elucidation of the unknown lytic substances.
    Type: Dataset
    Format: application/zip, 368.1 kBytes
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  • 4
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    PANGAEA
    In:  Supplement to: Ma, Haiyan; Krock, Bernd; Tillmann, Urban; Cembella, Allan (2009): Preliminary characterization of extracellular allelochemicals of the toxic marine dinoflagellate Alexandrium tamarense using a Rhodomonas salina bioassay. Marine Drugs, 7(4), 497-522, https://doi.org/10.3390/md7040497
    Publication Date: 2023-01-13
    Description: Members of the marine dinoflagellate genus Alexandrium are known to exude allelochemicals, unrelated to well-known neurotoxins (PSP-toxins, spirolides), with negative effects on other phytoplankton and marine grazers. Physico/chemical characterization of extracellular lytic compounds of A. tamarense, quantified by Rhodomonas salina bioassay, showed that the lytic activity, and hence presumably the compounds were stable over wide ranges of temperatures and pH and were refractory to bacterial degradation. Two distinct lytic fractions were collected by reversed-phase solid-phase extraction. The more hydrophilic fraction accounted for about 2% of the whole lytic activity of the A. tamarense culture supernatant, while the less hydrophilic one accounted for about 98% of activity. Although temporal stability of the compounds is high, substantial losses were evident during purification. Lytic activity was best removed from aqueous phase with chloroform-methanol (3:1). A “pseudo-loss” of lytic activity in undisturbed and low-concentrated samples and high activity of an emulsion between aqueous and n-hexane phase after liquid-liquid partition are strong evidence for the presence of amphipathic compounds. Lytic activity in the early fraction of gel permeation chromatography and lack of activity after 5 kD ultrafiltration indicate that the lytic agents form large aggregates or macromolecular complexes.
    Type: Dataset
    Format: application/zip, 843.6 kBytes
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  • 5
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    In:  Supplement to: Ma, Haiyan; Krock, Bernd; Tillmann, Urban; Bickmeyer, Ulf; Graeve, Martin; Cembella, Allan (2011): Mode of action of membrane-disruptive lytic compounds from the marine dinoflagellate Alexandrium tamarense. Toxicon, 58(3), 247-258, https://doi.org/10.1016/j.toxicon.2011.06.004
    Publication Date: 2023-01-13
    Description: Certain allelochemicals of the marine dinoflagellate Alexandrium tamarense cause lysis of a broad spectrum of target protist cells but the lytic mechanism is poorly defined. We first hypothesized that membrane sterols serve as molecular targets of these lytic compounds, and that differences in sterol composition among donor and target cells may cause insensitivity of Alexandrium and sensitivity of targets to lytic compounds. We investigated Ca2+ influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes. The lytic compounds increased permeability of the cell membrane for Ca2+ ions even during blockade of Ca2+ channels with cadmium. Results of a liposome assay suggested that the lytic compounds did not lyse such target membranes non-specifically by means of detergent-like activity. Analysis of sterol composition of isolates of A. tamarense and of five target protistan species showed that both lytic and non-lytic A. tamarense strains contain cholesterol and dinosterol as major sterols, whereas none of the other tested species contain dinosterol. Adding sterols and phosphatidylcholine to a lysis bioassay with the cryptophyte Rhodomonas salina for evaluation of competitive binding indicated that the lytic compounds possessed apparent high affinity for free sterols and phosphatidylcholine. Lysis of protistan target cells was dose-dependently reduced by adding various sterols or phosphatidylcholine. For three tested sterols, the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol. Cholesterol comprised a higher percentage of total sterols in plasma membrane fractions of A. tamarense than in corresponding whole cell fractions. We conclude therefore that although the molecular targets of the lytic compounds are likely to involve sterol components of membranes, A. tamarense must have a complex self-protective mechanism that still needs to be addressed.
    Type: Dataset
    Format: application/zip, 164.3 kBytes
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  • 6
    Publication Date: 2023-01-30
    Description: The data represent species counts (cells L-1) of the three AZA-producing dinoflagellate species Azadinium spinosum, Az. poporum and Amphidoma languida (all members of the taxonomic family Amphidomataceae) of water samples taken during in total six different field expeditions on several research vessels (RV Heincke, RV Uthörn, RV Polarstern) and on in total five stationary sampling stations (Scapa Flow/Scotland, Cuxhaven/Germany, Helgoland/Germany, Wilhelmshaven/Germany, Sylt/Germany) between 2015 and 2019. The water samples have been taken using Niskin bottles (on research vessels attached to a CTD). After DNA extraction, the species cell numbers have been calculated by quantitative PCR (qPCR) analysis using respective standard curves. These samples gained from different geographical areas in the eastern North Atlantic have been analyzed as part of the RIPAZA Project (funded by the German BMBF; in cooperation with the Third Institute of Oceanography, Xiamen/China) and the results are presented and discussed in the doctoral thesis of Stephan Wietkamp (Suppl.Tab.S6, Suppl.Tab.S7). Aim of the project and especially of this data set was to provide first reference data on the biogeography (geographical distribution and seasonality) of toxigenic Amphidomataceae in the eastern North Atlantic.
    Keywords: Amphidoma languida; Azadinium; Azadinium poporum; Azadinium spinosum; Azaspiracids; Cuxhaven_WS; DATE/TIME; Dinoflagellates; DNA; Field observation; Germany; Helgoland_WS; LATITUDE; Location; LONGITUDE; North Atlantic; qPCR; QPCR; Quantitative real-time PCR (qPCR); ScapaFlow_WS; Scotland; Sylt_WS; Water sample; Wilhelmshaven_WS; WS
    Type: Dataset
    Format: text/tab-separated-values, 980 data points
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  • 7
    Publication Date: 2023-07-09
    Description: At each study station, surface water temperature (±0.2°C) and salinity (±0.06) were measured in situ by triplicate readings with a multiparameter probe (Horiba U-10, Japan). Using Niskin bottles, surface water samples were collected for quantification of plankton and estimations of chlorophyll a. For the former, 250 ml were fixed with Lugol's solution (1% final concentration) and kept in the dark until analysis under the microscope, while for chlorophyll a, a volume of 200–250 mL was filtrated onboard through filter GF/C and kept at -20°C.
    Keywords: BH0915_01; BH0915_02; BH0915_03; BH0915_04; BH0915_05; BH0915_06; BH0915_07; BH0915_08; BH0915_09; BH0915_10; BH0915_11; BH0915_12; BH0915_13; BH0915_14; BH0915_15; BH0915_16; BH0915_17; BH0915_18; BH0915_21; BH0915_22; BH0915_23; BH0915_24; BH0915_33; BH0915_34; Chlorophyll a; Ciliates; Coccolithophoridae; Diatoms; Dinoflagellates; Dr. Bernardo Houssay; Event label; Flagellates; IADO-PNA 0915; Latitude of event; Longitude of event; MULT; Multiple investigations; Patagonian shelf, Argentina; Paulinella ovalis; pH; Phytoplankton; Phytoplankton, biomass as carbon; Protista, heterotrophic; Salinity; Temperature, water; Xanthophyceae
    Type: Dataset
    Format: text/tab-separated-values, 326 data points
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  • 8
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    PANGAEA
    In:  Supplement to: John, Uwe; Tillmann, Urban; Hülskötter, Jennifer; Alpermann, Tilman J; Wohlrab, Sylke; Van de Waal, Dedmer B (2014): Intraspecific facilitation by allelochemical mediated grazing protection within a toxigenic dinoflagellate population. Proceedings of the Royal Society B-Biological Sciences, 282(1798), 9 pp, https://doi.org/10.1098/rspb.2014.1268
    Publication Date: 2023-08-03
    Description: Dinoflagellates are a major cause of harmful algal blooms, with consequences for coastal marine ecosystem functioning and services. Alexandrium tamarense is one of the most abundant and widespread toxigenic species in the temperate northern and southern hemisphere, and produces paralytic shellfish poisoning toxins as well as lytic allelochemical substances. These bioactive compounds may support the success of A. tamarense and its ability to form blooms. Here we investigate the impact of grazing on monoclonal and mixed set-ups of highly (Alex2) and moderately (Alex4) allelochemically active A. tamarense strains and on a non-allelochemically active conspecific (Alex5) by the heterotrophic dinoflagellate Polykrikos kofoidii. While Alex4 and particularly Alex5 were strongly grazed by P. kofoidii when offered alone, both strains grew well in the mixed assemblages (Alex4+Alex5 and Alex2+Alex5). Hence, the allelochemical active strains facilitated growth of the non-active strain by protecting the population as a whole against grazing. Based on our results, we argue that facilitation among clonal lineages within a species may partly explain the high genotypic and phenotypic diversity of Alexandrium populations. Populations of Alexandrium may comprise multiple cooperative traits that act in concert with intraspecific facilitation, and hence promote the success of this notorious harmful algal bloom species.
    Type: Dataset
    Format: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet, 233.2 kBytes
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  • 9
    Publication Date: 2024-01-12
    Description: The data represent species counts (cells L-1) of the three AZA-producing dinoflagellate species Azadinium spinosum, Az. poporum and Amphidoma languida (all members of the taxonomic family Amphidomataceae) of water samples taken during in total six different field expeditions on several research vessels (RV Heincke, RV Uthörn, RV Polarstern) and on in total five stationary sampling stations (Scapa Flow/Scotland, Cuxhaven/Germany, Helgoland/Germany, Wilhelmshaven/Germany, Sylt/Germany) between 2015 and 2019. The water samples have been taken using Niskin bottles (on research vessels attached to a CTD). After DNA extraction, the species cell numbers have been calculated by quantitative PCR (qPCR) analysis using respective standard curves. These samples gained from different geographical areas in the eastern North Atlantic have been analyzed as part of the RIPAZA Project (funded by the German BMBF; in cooperation with the Third Institute of Oceanography, Xiamen/China) and the results are presented and discussed in the doctoral thesis of Stephan Wietkamp (Suppl.Tab.S6, Suppl.Tab.S7). Aim of the project and especially of this data set was to provide first reference data on the biogeography (geographical distribution and seasonality) of toxigenic Amphidomataceae in the eastern North Atlantic.
    Keywords: Amphidoma languida; ARK-XXIX/1, TRANSSIZ; Azadinium; Azadinium poporum; Azadinium spinosum; AZAHAB; Azaspiracids; Baltic Sea; Cells, total; Celtic Sea; CT; CTD, towed system; CTD/Rosette; CTD-RO; CTD-twoyo; DATE/TIME; Dinoflagellates; DNA; English Channel; Event label; Field observation; HE516; HE516_10-2; HE516_1-1; HE516_11-1; HE516_12-1; HE516_13-1; HE516_14-1; HE516_15-1; HE516_16-1; HE516_17-1; HE516_18-1; HE516_19-1; HE516_20-1; HE516_21-1; HE516_2-2; HE516_22-1; HE516_23-1; HE516_24-1; HE516_25-1; HE516_26-1; HE516_27-1; HE516_28-1; HE516_29-1; HE516_30-1; HE516_3-1; HE516_31-1; HE516_32-1; HE516_33-1; HE516_34-1; HE516_35-1; HE516_36-1; HE516_37-1; HE516_38-1; HE516_39-1; HE516_40-1; HE516_4-1; HE516_41-1; HE516_42-1; HE516_43-1; HE516_44-1; HE516_45-1; HE516_46-1; HE516_47-1; HE516_48-1; HE516_49-1; HE516_50-2; HE516_5-1; HE516_51-1; HE516_52-1; HE516_53-1; HE516_54-1; HE516_55-1; HE516_56-1; HE516_57-1; HE516_58-1; HE516_59-1; HE516_60-1; HE516_6-1; HE516_61-1; HE516_62-1; HE516_63-1; HE516_64-1; HE516_65-1; HE516_66-1; HE516_67-1; HE516_68-1; HE516_69-2; HE516_70-1; HE516_7-1; HE516_71-1; HE516_72-1; HE516_73-1; HE516_74-2; HE516_75-2; HE516_8-1; HE516_9-2; HE517; HE517_10-1; HE517_11-3; HE517_1-2; HE517_12-1; HE517_13-2; HE517_14-1; HE517_15-1; HE517_16-1; HE517_17-1; HE517_19-1; HE517_21-1; HE517_22-1; HE517_23-1; HE517_25-1; HE517_26-1; HE517_27-1; HE517_28-1; HE517_30-1; HE517_35-2; HE517_36-2; HE517_37-1; HE517_8-1; HE517_9-1; HE534; HE534_11-3; HE534_1-4; HE534_22-3; HE534_28-4; HE534_30-2; HE534_33-4; HE534_36-4; HE534_38-5; HE534_42-5; HE534_4-3; HE534_43-1; HE534_47-2; HE534_8-4; HE541; HE541_105-1; HE541_36-1; HE541_57-1; HE541_75-1; Heincke; Kattegat; LATITUDE; LONGITUDE; North Atlantic; North Sea; Polarstern; PS92; PS92-track; qPCR; QPCR; Quantitative real-time PCR (qPCR); Reference/source; South Atlantic Ocean; The Great Belt; Underway cruise track measurements; UT1606; UT1606/01-1; UT1606/02-1; UT1606/03-1; UT1606/04-1; UT1606/05-1; UT1606/06-1; UT1606/07-1; UT1606/08-1; UT1606/09-1; UT1606/10-1; UT1606/11-1; UT1606/12-1; UT1606/13-1; UT1606/14-1; UT1606/15-1; UT1606/16-1; UT1606/17-1; UT1606/18-1; UT1606/19-1; UT1606/20-1; UT1606/21-1; UT1606/22-1; UT1606/23-1; UT1606/24-1; UT1606/25-1; UT1606/26-1; UT1606/27-1; UT1606/28-1; UT1606/29-1; UT1606/30-1; UT1606/31-1; UT1606/32-1; UT1606/33-1; UT1606/34-1; UT1606/35-1; UT1606/36-1; UT1606/37-1; UT1606/38-1; UT1606/39-1; UT1606/40-1; UT1606/41-1; UT1606/42-1; UT1606/43-1; UT1606/44-1; Uthörn
    Type: Dataset
    Format: text/tab-separated-values, 995 data points
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  • 10
    Publication Date: 2024-02-07
    Description: The data set was acquired during the PHYCOB cruise in the frame of the Eurofleets+ programme (https://www.eurofleets.eu/2021/10/22/phycob-an-international-oceanographic-expedition-into-the-western-black-sea-coordinated-by-the-alfred-wegner-institut-helmholtz-zentrum-fur-polar-und-meeresforschung-awi/) within the Regional call in the territorrial waters of Romania and Bulgaria in the Western Baltic Sea from 11th to 17th September 2021. The aim of the cruise was to assess the occurrence of potentially harmfal algae and their associated phycotoxins in the Black Sea and the accompanying environmental parameters. Sampling was performed by CTD casts and Rosette water sampling in addition to vertical phytoplankton net hauls from 30 m depth to surface. The determined parameters include the following: Quantitative phytoplankton cell counts in water samples, qulitative determination of toxigenic species next generation sequencing data in plankton net concentrates, monoclonanal cultures established from water sample isolates, phycotoxins in plankton net concentrates, inorganic nitruinets (nitrate/nitrite, phosphate, silicate) , vitamin B12, particulate organic carbon, particulate organic nitrogen, flowcytometry data, and CTD data (temperature, salinity, chlorophyll-a, oxygen, turbidity, radiance).
    Keywords: chlorophyll-a; CTD profile; HAB species; isolated strains; net tows; NOC; Nutrient data; phycotoxins; POC; vitamin B12
    Type: Dataset
    Format: application/zip, 8 datasets
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