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  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 54 (1998), S. 458-460 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Human glutathione-S-transferase M2–2 (hGSTM2–2) was expressed in Escherichia coli and purified by GSH-affinity chromatography. The recombinant enzyme and the protein isolated from human tissue were indistinguishable based on physicochemical, enzymatic and immunological criteria. The catalytically active dimeric hGSTM2–2 was crystallized without GSH or other active-site ligands in two crystal forms. Diffraction from form A crystals extends to 2.5 Å and is consistent with the space group P21 (a = 53.9, b = 81.5, c = 55.6 Å, β = 109.26 Å) with two monomers in the asymmetric unit. Diffraction from form B crystals extends to 3 Å and is consistent with a space group P212121 (a = 57.2, b = 80.7, c = 225.9 Å) with two dimers in the asymmetric unit. This is the first report of ligand-free mu-class GST crystals, and a comparison with liganded complexes will provide insight into the structural consequences of substrate binding which are thought to be important for catalysis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] With many genomes sequenced, a pressing challenge in biology is predicting the function of the proteins that the genes encode. When proteins are unrelated to others of known activity, bioinformatics inference for function becomes problematic. It would thus be useful to interrogate protein ...
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Regulation of T-cell activity is dependent on antigen-independent co-stimulatory signals provided by the disulphide-linked homodimeric T-cell surface receptors, CD28 and CTLA-4 (ref. 1). Engagement of CD28 with B7-1 and B7-2 ligands on antigen-presenting cells (APCs) provides a ...
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The Ena-VASP homology (EVH1) domain is a protein interaction module found in several proteins that are involved in transducing migratory and morphological signals into cytoskeletal reorganization. EVH1 specifically recognizes proline-rich sequences in its binding partners and directs the ...
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature America Inc.
    Nature structural biology 6 (1999), S. 666-671 
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The actin regulatory protein profilin is targeted to specific cellular regions through interactions with highly proline-rich motifs embedded within its binding partners. New X-ray crystallographic results demonstrate that profilin, like SH3 domains, can bind proline-rich ligands in two distinct ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature structural biology 4 (1997), S. 708-712 
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Sir — A large superfamily of cytoskeletal proteins is responsible for the localization and crosslinking of filamentous actin (F-actin) into higher order assemblies such as bundles and networks. These structures are involved in cell motility, cytokinesis, cell morphogenesis, muscle sarcomere ...
    Type of Medium: Electronic Resource
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  • 7
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    PANGAEA
    In:  Supplement to: Beer, Christian; Fedorov, Alexander N; Torgovkin, Y (2013): Permafrost temperature and active-layer thickness of Yakutia with 0.5-degree spatial resolution for model evaluation. Earth System Science Data, 5(2), 305-310, https://doi.org/10.5194/essd-5-305-2013
    Publication Date: 2023-01-13
    Description: Based on the map of landscapes and permafrost conditions in Yakutia (Merzlotno-landshaftnaya karta Yakutskoi0 ASSR, Gosgeodeziya SSSR, 1991), rasterized maps of permafrost temperature and active-layer thickness of Yakutia, East Siberia were derived. The mean and standard deviation at 0.5-degree grid cell size are estimated by assigning a probability density function at 0.001-degree spatial resolution. Spatial pattern of both variables are dominated by a climatic gradient from north to south, and by mountains and the soil type distribution. Uncertainties are highest in mountains and in the sporadic permafrost zone in the south. The maps are best suited as a benchmark for land surface models which include a permafrost module.
    Keywords: Central_Yakutia; Description; File name; File size; File type; Sakha Republic, Russia; Uniform resource locator/link to file
    Type: Dataset
    Format: text/tab-separated-values, 20 data points
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  • 8
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    PANGAEA
    In:  Supplement to: Nitze, Ingmar; Grosse, Guido; Jones, Benjamin M; Arp, Chistopher D; Ulrich, Mathias; Fedorov, Alexander N; Veremeeva, Alexandra (2017): Landsat-Based Trend Analysis of Lake Dynamics across Northern Permafrost Regions. Remote Sensing, 9(7), 640, https://doi.org/10.3390/rs9070640
    Publication Date: 2023-06-30
    Description: Lakes are a ubiquitous landscape feature in northern permafrost regions. They have a strong impact on carbon, energy and water fluxes and can be quite responsive to climate change. The monitoring of lake change in northern high latitudes, at a sufficiently accurate spatial and temporal resolution, is crucial for understanding the underlying processes driving lake change. To date, lake change studies in permafrost regions were based on a variety of different sources, image acquisition periods and single snapshots, and localized analysis, which hinders the comparison of different regions. Here we present, a methodology based on machine-learning based classification of robust trends of multi-spectral indices of Landsat data (TM,ETM+, OLI) and object-based lake detection, to analyze and compare the individual, local and regional lake dynamics of four different study sites (Alaska North Slope, Western Alaska, Central Yakutia, Kolyma Lowland) in the northern permafrost zone from 1999 to 2014. Regional patterns of lake area change on the Alaska North Slope (-0.69%), Western Alaska (-2.82%), and Kolyma Lowland (-0.51%) largely include increases due to thermokarst lake expansion, but more dominant lake area losses due to catastrophic lake drainage events. In contrast, Central Yakutia showed a remarkable increase in lake area of 48.48%, likely resulting from warmer and wetter climate conditions over the latter half of the study period. Within all study regions, variability in lake dynamics was associated with differences in permafrost characteristics, landscape position (i.e. upland vs. lowland), and surface geology. With the global availability of Landsat data and a consistent methodology for processing the input data derived from robust trends of multi-spectral indices, we demonstrate a transferability, scalability and consistency of lake change analysis within the northern permafrost region.
    Keywords: AKS_lakes; Alaska North Slope; AWI_PerDyn; Central Yakutia; CYA_lakes; Elevation of event; Event label; File size; Kobuk-Selawik-Lowlands; KOL_lakes; Kolyma Lowland; Latitude of event; Longitude of event; NSL_lakes; Permafrost Research (Periglacial Dynamics) @ AWI; Uniform resource locator/link to file
    Type: Dataset
    Format: text/tab-separated-values, 8 data points
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  • 9
    Publication Date: 2023-06-27
    Description: The dataset presents the radiocarbon ages (with standard deviation ±) from thermokarst lake sediment cores from the Yukechi Alas, Siberia. The sediment cores were taken from an alas lake (YUK15-YU-L7) and a yedoma lake (YUK15-YU-L15). Raw, calibrated and rounded calibrated ages are presented. Measurements were carried out in the AWI MICADAS Laboratory in Bremerhaven. Calibrations were performed according to Stuiver et al. (2017) using CALIB 7.1 software and the IntCal13 calibration curve.
    Keywords: AGE; Age, 14C AMS, Mini Carbon Dating System (MICADAS); Age, 14C calibrated, CALIB 7.1 (Stuiver et al. 2017) and IntCal13 calibration curve; Age, dated; Age, dated material; Age, dated standard deviation; Age, standard deviation; Alas lake; Arctic; AWI_PerDyn; AWI Arctic Land Expedition; Central_Yakutia_Yukechi_2015; Cruise/expedition; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Event label; Lake type; LATITUDE; LONGITUDE; Permafrost Research (Periglacial Dynamics) @ AWI; radiocarbon ages; RCDT; RU-Land_2015_CentralYakutia_Yuke; Sample ID; Sediment type; thermokarst lake; Truck mounted rotary drill; Yakutia; Yedoma lake; YUK15-YU-L15; YUK15-YU-L7
    Type: Dataset
    Format: text/tab-separated-values, 143 data points
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  • 10
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    PANGAEA
    In:  Supplement to: Windirsch, Torben (2018): Organic matter characteristics in a changing permafrost environment: Yukechi Alas landscape, Central Yakutia. University Potsdam, master thesis, hdl:10013/epic.2e1a8ca1-3b83-4d7f-8c7d-9d693370d7dc
    Publication Date: 2023-11-06
    Description: These data are laboratory results for carbon characteristics (TOC, TC, TN, C/N ratio, stable carbon isotope ratio, radiocarbon ages from bulk organic material), sediment characteristics (mass specific magnetic susceptibility (4.65 kHz & 0.465 kHz), grain size measurements 〈 1 mm) and ice characteristics (sample mass (wet), sample mass (dry), absolute ice content, stable oxygen and hydrogen isotope ratios of pore ice, pH and conductivity of pore ice). Two cores were analyzed: Yedoma core YED1 N 61.75967° / E 130.47438°; h = 218 m asl total length: 22,35 m (recovery 21,13 m) ice wedge included from ~7,14 to ~9,5 m Alas core Alas1 drilled in the drained part of a thermokarst lake basin N 61.76490° / E 130.46503° total length: 19,80 m (recovery 11,14 m) unfrozen conditions from ~1,6 to ~7,5 m Both cores were drilled in March 2015 from dry land surface in a joint expedition of the ERC PETA-Carb poject and the DFG project UL426/1-1 "Short and long-term thermokarst dynamics due to climate changes and human impacts in Central Yakutia, Siberia (2013-2016)". The sample names are compiled as [campaign]-[core] [drive] [depth (top)] - [depth (bottom)]. Age calibration was done with Calib7.10. Grain size percentages were calculated using GRADISTAT (Blott and Pye, 2001). Missing carbon isotope and C/N ratio values are a result of carbon and/or nitrogen contents below detection limit (0.1 wt%). Missing ice characteristics values are a result of dry and/or fine material obstructing the pore ice/water extraction.
    Keywords: Alas; AWI_PerDyn; Carbon; Central Yakutia; Permafrost; Permafrost Research (Periglacial Dynamics) @ AWI; PETA-CARB; Rapid Permafrost Thaw in a Warming Arctic and Impacts on the Soil Organic Carbon Pool; Yedoma; Yukechi
    Type: Dataset
    Format: application/zip, 8 datasets
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