Publication Date:
1988-10-28
Description:
The alpha and beta subunits of the gamma-aminobutyric acidA (GABAA) receptor were expressed individually in Xenopus oocytes by injection of RNA synthesized from their cloned DNAs. GABA-sensitive chloride channels were detected several days after injection with any one of three different alpha RNAs (alpha 1, alpha 2, and alpha 3) or with beta RNA. The channels induced by each of the alpha-subunit RNAs were indistinguishable, they had multiple conductance levels (10, 19, 28, and 42 picosiemens), and their activity was potentiated by pentobarbital and inhibited by picrotoxin. The beta channels usually expressed poorly but showed similar single channel conductance levels (10, 18, 27, and 40 picosiemens), potentiation by pentobarbital and inhibition by picrotoxin. The finding that both alpha and beta subunits, examined separately, form GABA-sensitive ion channels with permeation properties and regulatory sites characteristic of the native receptor suggests that the amino acid sequences that confer these properties are within the homologous domains shared by the subunits.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Blair, L A -- Levitan, E S -- Marshall, J -- Dionne, V E -- Barnard, E A -- NS20962/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1988 Oct 28;242(4878):577-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉MRC Molecular Neurobiology Unit, University of Cambridge, United Kingdom.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2845583" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Chloride Channels
;
Chlorides/*physiology
;
Cloning, Molecular
;
Dose-Response Relationship, Drug
;
Electric Conductivity
;
Macromolecular Substances
;
Membrane Proteins/*physiology
;
Picrotoxin/pharmacology
;
RNA, Messenger/administration & dosage
;
Receptors, GABA-A/*physiology
;
Structure-Activity Relationship
;
Xenopus laevis
;
gamma-Aminobutyric Acid/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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