ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Column liquid chromatography  (119)
  • nitrogen  (53)
  • Springer  (172)
  • American Geophysical Union
  • MDPI Publishing
  • Springer Science + Business Media
  • 1990-1994  (172)
  • 1993  (172)
Collection
Keywords
Publisher
  • Springer  (172)
  • American Geophysical Union
  • MDPI Publishing
  • Springer Science + Business Media
  • Wiley-Blackwell  (2)
Years
  • 1990-1994  (172)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 35 (1993), S. 217-226 
    ISSN: 1573-0867
    Keywords: calcium ; dry matter distribution ; fertilizer ; harvest index ; magnesium ; manure ; millet ; nitrogen ; nutrient uptake ; phosphorus ; potassium ; Senegal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a fertilizer and manure experiment, millet was grown under four treatments (no fertilizer or manure, farmyard manure, chemical fertilizer, and both). Grain yield and total aboveground biomass production of the unfertilized plot were relatively high. The observed differences in total dry matter production must be attributed to differences in nutrient availability, as amount of rainfall and its distribution were favourable. Results show only small differences in distribution of dry matter among the various plant organs between the best and the non-fertilized treatments. Nutrient supply from natural sources, defined as crop content of N, P, and K at maturity without fertilizer application, amounted to 104, 16 and 103 kg ha−1, respectively, which are very high values. Total uptake of calcium and magnesium is related to that of potassium, as the combined content of these three elements is linearly related to total aboveground biomass production. Minimum removal of nitrogen and phosphorus per ton grain dry matter amounts to 29 and 4kg, respectively, and 9 kg potassium per ton total aboveground dry matter. A possible double function of phosphorus as element of structural biomass and for maintenance of electro-neutrality is discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 34 (1993), S. 121-126 
    ISSN: 1573-0867
    Keywords: Controlled release fertilizers ; electroultrafiltration ; nitrogen ; ryegrass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The efficiency of different nitrogenous fertilizers under forced leaching conditions was determined in pot experiments using ryegrass as an indicator plant in a sandy loam soil. Treatments were: ON (unfertilized), AN (ammonium nitrate), U (urea), DAP (diammonium phosphate), T (Triabon), FK (Floranid Komplett) and CDU (crotonylidendiurea). N source effects on N uptake, and N leached (as % of N applied) were evaluated. Results show that the fertilizers which behaved best with regard to N uptake were FK followed by Triabon and AN. The most soluble fertilizers, AN and urea, gave the highest N leaching losses. Significant correlations between EUF-NI (rapidly soluble fraction) and leaching, as well as between EUF-N (I + II) and N uptake by the plant (where EUF-N II relates to the slowly soluble fraction) were obtained.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1572-9680
    Keywords: alley cropping ; maize ; nitrogen ; organic matter ; soil fertility ; Leucaena leucocephala ; Flemingia congesta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A detailed study of the soil chemical and physical properties in seven-year-old alley cropping trial containingLeucaena leucocephala andFlemingia congesta in Northern Zambia is described. There was a strong correlation between the maize yield and the total amount of nitrogen applied, both from prunings and fertiliser, suggesting that a major reason for the observed benefit from alley cropping, particularly withLeucaena, was due to an improvement in nitrogen supply.Leucaena produced significantly more biomass, and its leaves had higher concentrations of nitrogen, phosphorous and potassium and lower C/N and C/P ratios than did those ofFlemingia. There was also evidence that the trees had a beneficial effect on other soil chemical properties; under the hedgerows, particularly those ofLeucaena, there were higher levels of organic carbon, Mg, K and ECEC, and pH values were also highest. It is suggested that higher levels of organic carbon in the alley crop treatments were responsible for the improvements observed in soil physical properties. Lower bulk density, lower penetration resistance, and a higher infiltration rate and pore volume fraction were measured in the alley crops, although there was no significant change in the soil water release parameters. A deteriorating effect of constant applications of nitrogen fertiliser on soil fertility was observed; as the level of urea application increased, there were significant decreases in Mg, K and pH, increases in Al and soil acidity, and higher penetrometer resistance. These results highlight the urgent need for further research on biological methods of maintaining soil fertility.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Aquatic sciences 55 (1993), S. 132-142 
    ISSN: 1420-9055
    Keywords: nitrogen ; phosphorus ; uptake ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dissolved inorganic nutrient pools are small relative to particulate pools, and dissolved pools turnover rapidly. It has been observed that pools change little from day to day on the sampling scales usually employed. A simple model is presented where uptake and regeneration rates balance to cause a local steady state concentration for dissolved inorganic nutrients. Enrichment and dilution perturbation experiments with lake water support the idea of steady state nutrient concentrations. Although inorganic nutrient concentrations are often controlled by biota, the absolute concentrations present tell little about the activity of that biota.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1423-0445
    Keywords: carbon/nutrient balance hypothesis ; carbon allocation ; fertilization ; phenolics ; nitrogen ; terpene ; Abies grandis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrogen fertilization resulted in a linear increase in the growth ofAbies grandis seedlings, but linear decrease in foliage concentrations of phenolic compounds. These data are consistent with the inverse relationship between growth and production of carbonbased secondary chemicals predicted by the carbon/nutrient balance (CNB) hypothesis. However, in contrast to predictions of the CNB hypothesis, nitrogen fertilization had no effect on foliage terpene concentrations. The results suggest that not all carbon-based chemicals respond in the same manner to environmental variation, and that the carbon/nutrient balance hypothesis does not adequately explain all patterns of environmentally-induced variation in secondary metabolism.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1573-1561
    Keywords: Artificial diet ; herbivory ; nitrogen ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; monoterpenes ; nutritional ecology ; phenolics ; Pseudotsuga menziesii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two experiments were conducted to examine the influence of foliar nitrogen, terpenes, and phenolics of Douglas-fir on the development of gypsy moth larvae. In the first experiment, foliar concentrations of nitrogen and allelochemicals were manipulated by fertilizing 3-year-old potted seedlings with 0 or 200 ppm nitrogen. Concentrations of foliar nitrogen (0.33–2.38%) were negatively correlated with the phenolics (15.8–24.4 mg/g). Sixth-instar larvae previously reared on current-year Douglas-fir needles were allowed to feed on these seedlings. Pupal weights (312.8–995.6 mg) were positively correlated with levels of foliar nitrogen, negatively correlated with amounts of foliar phenolics, and uncorrelated with terpene concentrations. In the second experiment, terpene and phenolic extracts from Douglas-fir foliage were incorporated at natural levels into artificial diets with high and low levels of protein nitrogen. Neonate larvae grew faster and were larger on the high nitrogen control diet (4.1–4.5%), however, fourth instars performed better on the control diet with low nitrogen levels (2.5–2.7%). Foliar terpenes incorporated into diet had little effect on neonate fitness, but may induce subtle physiological changes in later instar larvae. Phenolics, alone or in combination with terpenes, excessively suppressed growth and survival, with no individuals living through the fourth instar, regardless of the nitrogen level. Incorporating foliar phenolic extracts into artificial diet caused unnatural levels of toxicity and failed to clarify the effects of Douglas-fir phenolics on gypsy moth fitness. Foliar nitrogen is a key factor influencing gypsy moth development on Douglas fir, but may be mitigated to some degree by phenolics.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Bovine milk ; Multivariate optimization ; Glycerophospholipids ; Molecular species separation ; Chemometrics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The molecular species profile, representing the fatty acid combinations on the glycerol backbone, is the most fundamental description of an intact glycerophospholipid class and provides a key to understanding its functional characteristics. We have developed a powerful RP-HPLC method for the separation of molecular species, including so-called “critical pairs”, of natural mixtures of 1,2-diacyl-sn-glycero-3-phosphocholine 1,2-diacyl-sn-glycero-3-phosphoethanolamine from bovine milk and other natural sources. We have used a novel multivariate development and optimization strategy to locate the region of optimum separation conditions within a prechosen domain of the chromatographic parameter space.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Open tubular columns ; Cross-linked polyacrylate stationary phase ; Photopolymerization ; Antracene derivatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Polyacrylate films containing a lauryl moiety were fabricated on about 12 μm I.D. fused silica capillaries by in-situ photopolymerization of acrylates. Relatively thick films up to 1 μm were obtained by the technique. The films were tested as retentive layers for reversed phase open tubular liquid chromatography (OTLC). Due to the presence of the incorporated lauryl moiety in the layer considerable retention could be achieved for polar compounds with aqueous mobile phases. The efficiency of the prepared columns approximates to the theoretical value. The polyacrylate films appear to be extremely stable even under strong basic conditions. The films swell considerably in the presence of nonpolar solvents resulting in more favourable retention and a significant improvement of the chromatographic performance.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 627-630 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Fatty acid analysis ; Fish oils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A chromatographic method has been developed for the determination of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in fish oil dietary supplements containing triglycerides rich in (n−3) polyunsaturated fatty acids (PUFAs). Following the ester saponification, free fatty acids were resolved by TLC into two fractions: i) a more mobile group of saturated and monounsaturated fatty acids and ii) a less mobile group containing PUFAs. The PUFA fraction was further analyzed by HPLC to determine the levels of EPA and DHA in the fish oil sample.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbidopa and levodopa in plasma ; Electrochemical detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A validated reversed-phase high-performance liquid chromatographic procedure employing electrochemical detection (LCEC) for the analysis of carbidopa and levodopa in human plasma is reported. The method is sensitive and specific with amperometric detection at a glassy carbon working electrode with Eapp=0.75 V vs. Ag/AgCl. The retention times of levodopa, internal standard, and carbidopa are 3.3, 4.5, and 9.7 minutes, respectively, with an overall chromatographic run time of 12.0 minutes. The peak height ratio versus plasma concentration is linear over the range of 5.0 to 500 ng/mL for each analyte and exhibits correlation coefficients of 0.9957 or better (n=9). The mean absolute recovery of carbidopa and levodopa using the described assay is 36.6 and 66.0%, respectively. The inter- and intra-day accuracy and precision are within 11.8% of the actual values for all concentrations. Also, due to the demonstrated instability of carbidopa and levodopa in plasma a procedure is provided to circumvent this. Blood collected in pre-treated Vacutainer tubes can be stored in an ice bath for up to 4 hours without any significant degradation, thereby providing a practical means for processing several clinical samples simultaneously.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 11
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Fullerenes ; FullereneSep® ; Separation factor ; HPLC-particle beam mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary High-performance liquid chromatographic separation of fullerene mixtures in the range C60 to C100 can be achieved using a novel stationary phase which rivals the performance of the more expensive ‘Pirkle-type’ columns currently employed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 675-678 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ion chromatography ; Trace metal determination ; Sample contamination ; Human hair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The dynamic ion-exchange chromatographic analysis of bivalent ions, such as Cu, Pb, Zn, Ni, Co and Mn in resistant organic matrices needs preliminary wet ashing with oxidizing acids (HNO3 and HClO4). Such treatment may lead to an increase in the amounts of metals present in the sample due to their release from containers or equipment used in an acidic environment and to metal impurities contained in the acids. The effect of these interferences in the determination of trace metals in human hair was studied.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 13
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; GC/ECD detection ; PCBs, ortho and non-ortho ; Chromatographic separation ; Congener determination ; FTIR identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An analytical procedure for the individual determination of ortho and non-ortho PCB congeners in sediments, using high performance liquid chromatography (HPLC) preseparation and gas chromatography/ECD detection, is described. Gas chromatography/FTIR spectrometry (GC/FTIR) and gas chromatography/mass spectrometry (GC/MS) were employed for individual congener identification and determination. Sample extraction, clean-up of extract and selective elution procedures were optimized by using reference certified marine sediment samples. Recovery and precision were typically 83% and 16% respectively at 2 ng/g of total PCB content. The proposed procedure, tested by analyzing real sediment samples, showed a reproducibility better than 20% at 13 ng/g PCB level.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 14
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Glutamine ; Pyroglutamic acid ; Amino acids formulations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The aminoacid glutamine in aqueous solution and in conditions of high temperature and long term storage is partly transformed into pyroglutamic acid which exhibits potential neurotoxic effects. Commercially available aminoacid mixtures supplemented with glutamine are heat-sterilized and some losses of glutamine and formation of pyroglutamic acid may occur. The aim of the work was to set up an easy and reliable HPLC method which allows the determination of pyroglutamic acid as a degradation product of glutamine. The column was a 5 μm Hypersil ODS (100×4.6 mm) and the mobile phase 100% 0.007 M phosphate buffer pH 3.5. Stability studies in different conditions of temperature and time of storage were performed on aminoacid mixture available in the commerce.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 15
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbonyl compounds ; Luminarin hydrazides ; Fluorescence detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Fluorogenic reagents (luminarin 3, luminarin 11 and luminarin 12), having a quinolizinocoumarin moiety as fluorophore and a carboxylic acid hydrazide function as reacting group, have been developed. These reagents were found to be highly sensitive fluorescence derivatization reagents for aldehydes and ketones in high-performance liquid chromatography. The reagents readily react with carbonyl compounds in aqueous sulphuric acid solution (0.1 M) at room temperature to produce the corresponding hydrazone derivatives, which can be separated on both reversed or normal-phase column. The structures of the derivatives were studied, together with their properties in reversed and normalphase chromatographic systems. UV absorbance, corrected fluorescence spectral data and quantum yields of luminarin 3, luminarin 11 and luminarin 12 are presented. The detection limits (signal to noise ratio=3) for aldehydes and ketones were in the sub-pmol range. Luminarin 3 was also applied to the determination of hydroxymethylfurfural (HMF) in orange juices and concentrates. The method for HMF involves the solid-liquid extraction of the juice by using a C-18 cartridge prior to derivatization and normal-phase separation of the derivative with fluorimetric detection at 387 nmex., 444 nm em. The calibration curve was linear for amounts of HMF ranging from 0.1 to 10 nmol. Intrarun relative standard deviation was 12.8% for 0.1 nmol and 2.6% for 1 nmol. Recovery studies indicated an average of 98.7±1.9% for juice concentrate and 99.8±3.2% for pasteurized juice.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 16
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Method development ; Chemometrics ; Response criteria ; Analysis of drug purity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Optimization procedures require adequate response criteria to assess the quality of each chromatogram obtained during the process. The objective of this paper is to evaluate the possibility of using different resolution functions, in the chromatographic separation of a drug and its impurities (particularly the impurity just eluted after the drug). This study shows the limits of some resolution expressions. The interest of the simple Δt criterion, the difference between retention times, is presented in this paper.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 36 (1993), S. 147-151 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Polarity ; Retinoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The retention of retinoids in reversed-phase liquid chromatography was studied using aqueous mobile phases of different composition (methanol 94–86% and acetonityrile 92–82%) at five temperatures (40–60 °C). With both organic modifiers the effect of the molecular structure increased as the water content and the polarity of the mobile phase increased. The temperature-dependence increased in the same manner with aqueous acetonitrile mobile phases. The π-π interactions between the retinoids and acetonitrile diminish when the water content of the mobile phase is increased, as happens also to the hydrophobic interactions with both organic modifiers. The net effect of these changes depends on the composition of the mobile phase. There was excellent correlation of retention with all polarity parameters studied(ϕ, P′, xe, xd, xn, E T N , δT, δ, δo and δd), when the calculations were made separately with methanol and acetonitrile. The volume fraction of the organic modifier, ϕ, was the only parameter describing the retention well in both organic modifiers simultaneously.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 18
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Aromatic amines ; Arylbenzylamide methylthioether derivative ; SL 85.1016 ; Pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An HPLC method has been developed for the determination of SL 85.1016, a new calcium antagonist arylbenzylamide methylthioether derivative. SL 85.1016 and the internal standard, SL 87.0210, are extracted from alkaline human plasma withn-hexane and back extracted into 0.05 M phosphate buffer (pH 2.5; 0.2 ml). Acetonitrile (50 μl) is added to the final aqueous extract in order to prevent absorption of the compounds of interest onto the walls of the glass tube; this solution then is partially processed by HPLC on a C18 column with UV detection (254 nm). The determination limit of the method is 2 ng.ml−1 of SL 85.1016 in human plasma; the response to the drug is linear in the range 2–200 ng.mg−1.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 36 (1993), S. 347-350 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Allium ; Derivatization ; S-alk(en)yl-L-cysteine sulfoxides ; Sulfur amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Two pre-column derivatization procedures coupled with reversed phase HPLC have been compared for the analysis ofS-Alk(en)yl0L-cysteine sulfoxides in variousAllium species. In order to establish external standards some (+/-) sulfoxides were synthesized, using a new method to enhance asymmetric synthesis of the diastereoisomers. The first derivatization method is the formation ofo-phthaldialdehyde/tert.-butylthiol derivatives which can be analyzed using UV detection. The second, presently used for amino acid analysis, is the Waters Pico-Tag method, which employs phenylisothiocyanate as derivatization reagent. As the Pico-Tag method was found to be the most efficient for determination ofS-alk(en)yl-L-cysteine sulfoxides it was used to determine the alliin content of various samples of garlic.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 20
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chlortetracycline ; Reversed-phase silical gel ; C8 bonded phase ; Collaborative study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An established method for the analysis of chlortetracycline by liquid chromatography using octylsilylated silica gel as the stationary phase was examined in a multicentre study involving five laboratories and a total of six columns. Three chlortetracycline hydrochloride samples were analysed. The main component and the impurities were determined. An analysis of variance, treating each column as a different laboratory, showed an absence of consistent between-laboratory bias and the presence of a significant laboratory-sample interaction. The repeatability and the reproducibility of the method, expressed as relative standard deviations of the result of the determination of chlortetracycline hydrochloride, were calculated to be 0.9% and 1.1% respectively.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 21
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 37 (1993), S. 627-634 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; ODS phases ; NMR and FTIR characterization ; Stationary phase functionality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Two spectroscopic methods, solid state NMR and diffuse reflectance FTIR, have been used in conjunction with liquid chromatographicretention data to identify and distinguish the functionality of octadecylsilica (ODS) stationary phases. NMR and FTIR spectra are indicative of various features of the different functionalities of the phases and this information can be used to elucidate the configuration of the organosilyl moiety in the ODS on the silica surface and explain why DSS phases have different molecular shape (mainly planarity) recognition capability.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 22
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 37 (1993), S. 423-428 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Polymer-modified silica ; Polypyrrole cloride ; Polypyrrole dodecylsulfate ; Small molecules ; Polyaromatic hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Polypyrrole chloride and polypyrrole dodecylsulfate coated-silica packing materials have been synthesized. Reverse-phase chromtographuy was employed to characterise both packings. A series of test compounds with known properties was used as molecular probes. They included benzene and derivatives, basic drugs, and polyaromatic hydrocarbons. A commericial C18 column was also used for the purpose of comparison in some cases.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 23
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chiralcel OD ; N-Alkyl-N-methylanilineN-oxide enantiomers ; Chiral nitrogen centre ; Flavin-containing monooxygenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A high-performance liquid chromatographic method for the resolution of the enantiomers of a series ofN-alkyl-N-methylanilineN-oxides is reported. The resolutions were achieved using a Chiralcel, OD chiral stationary-phase with a mobile-phase of hexane and ethanol in varying proportions. The chromatographic order of elution of the enantiomers ofN-ethyl-N-methylanilineN-oxide was determined to be (+)-(R) before (−)-(S).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 24
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 37 (1993), S. 539-542 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Sotolon ; Vin jaune ; Flor-sherry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Sotolon (4,5-dimethyl-3-hydroxy-2(5H)-furanone) is a key flavour compound in the french flor-sherry “Vin jaune”. This compound was determined quantitatively by extraction of 25 ml of wine on a XAD-4 resin, elution with diethyl ether, separation by HPLC on a Lichrospher 100 Diol column, elution with dichloromethane/hexane (60/40) and UV detection at 232 nm. The amount of sotolon in “Vin jaune” (120 to 268 μg/l) was related to the development of the yeast film over a period of 6 years. Only 6 to 51 μg/l were found in the “Vin de paille” which is made with overmaturated grapes of the same Savagnin vine-plant but without development of yeasts, and 80 to 140 μg/l in “Tokai” which are partly grown under a yeast film.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 25
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Immunoaffinity chromatography ; Proteins ; Surface plasmon resonance detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The BIAcoreTM Biosensor System utilizes a detection principle known as Surface Plasmon Resonance (SPR) which, in simple terms, detects changes in the refractive index of a solution in contact with a gold film. The gold film is surface modified with carboxymethylated dextran to produce a hydrophillic matrix onto which macromolecules may be covalently immobilized. In this respect, the BIAcoreTM is similar to any other insoluble matrix, such as a chromatographic support. The SPR detection principle, however, allows one to directly ‘visualize’ the interaction under study, in real time and without the need for reporter molecules such as enzyme-labels. In addition, the very small sample requirements, automated robotics unit and ease of data analysis suggest the potential use of the BIAcoreTM instrumentation for assay development and possibly process development, especially where bio-specific interactions such as immunoaffinity chromatography are used as a step in the process. In this report, we demonstrate the use of the BIAcoreTM SPR detector for the micro-scale determination of conditions for immunoaffinity chromatography of soluble complementreceptor 1, sCR1.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 26
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 5-12 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Post-column reaction detector ; Explosives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Improvements in selectivity and sensitivity in the analysis of common explosives, like nitrate esters, nitramines and nitroaromatic compounds can be achieved by post-column derivatisation in a two step reaction detector. The first step in derivatisation is the photolysis of the analytes with UV at 254 nm. The photo reactor consists of a crocheted 20 m Tefzel capillary, which is coiled around a low pressure mercury lamp. In second step the nitrite ion generated is subsequently detected by a colourimetric reaction. The azo dye formed can be selectively detected at 540 nm. Addition of alkali after chromatographic separation to prevent oxidation of initially formed nitrite to nitrate during photolysis leads to a complex multistage arrangement. However, the contribution to peak broadening by the reactor is negligible and it is possible to detect 25–50 ppb of nitramines and 30–100 ppb of nitrate esters. Another advantage of the method is the selective detection of nitro compounds, even in complex matrices. The trace analysis of explosives is of growing interest in forensic science as well as in environmental analysis. It has been shown [1] that explosives can easily be extracted from soil and debris by the use of supercritical carbon dioxide. The separation and determination of explosives by gas chromatography is hindered by their thermal instability. In HPLC only the nitro aromatic explosives can be detected with sufficient sensitivity. Other types of explosives like the esters of nitric acid or nitramines do not absorb sufficiently in the UV region for sensitive detection. It has been shown [2] that explosives are liable to photochemical decomposition in the UV region, resulting in nitrate and nitrite, which have been detected after separation by ion-pair chromatography with electrochemical detection. A more sensitive and selective detection of nitrite has been possible in flow injection analysis [3]. Here a modified Griess reaction has been used. In a first step nitrite ions are used to form the diazonium salt with sulfanilamide which is coupled in a second step with N-[naphthyl-(1)]-ethylene diamine (NED) to form a redviolet azo dye with an absorption maximum at 540 nm. The advantage of this method is selective detection in the visible region, where hardly any other organic components are detected, which might be present in a crude environmental sample. In this paper the transfer of the Griess reaction to post-column derivatisation in RP chromatography of explosives will be described, and the optimisation of trace analysis of these solutes will be discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 27
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reverse phase ; Optimization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An interpretive optimization procedure in which pH can be one of the variables is presented with the emphasis on optimizing separations. When varying the pH in reversed-phase liquid chromatography the retention of ionogenic solutes will change. Thus, the selectivity between ionogenic and neutral solutes or between ionogenic solutes mutually can be optimized. However, pH also greatly affects the efficiency (plate count) and peak shape (asymmetry). Optimum selectivity (i.e. large differences in retention times) may be observed under conditions where peaks are broad and asymmetrical. Thus, it is essential to simultaneously consider retention, peak width and peak shape and their effects on separation (effective resolution) in pH-optimization studies. A procedure in which this is done is presented and applied to optimizing the separation of a synthetic mixture of selected pharmaceuticals. After initial experiments to establish the parameter space (boundaries for pH and binary methanol — water composition), twelve experiments are performed according to a 3×4 experimental design. At each loaction the retention, peak height, peak area and peak symmetry are recorded for each solute. These data are then used to build models for each of the four characteristics and for each solute. From this set of models the response surface, describing the quality of separation as a function of pH and composition, can be calculated. A variety of optimization criteria (quantifying quality of separation) can be used. The optimum corresponds to the highest point on the response surface.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 28
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 38-44 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Fullerenes ; On-line and off-line identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The separation of fullerenes with a monomeric octadecylsilica bonded phase using n-hexane or toluene/methanol mobile phase systems is described. Analytical and preparative separations, incorporating on-line UV/VIS spectral measurements, confirmed the existence of large fullerenes such as C76, C78 and C84. However, isomers of C78 and C84 were not conclusively found.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 29
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 50-54 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Wine ; Volatile phenols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An alternative to the traditional solvent extraction method used to extract and rapidly quantify ethyl-and vinylphenol and ethyl-and vinylgaiacol from wine is presented. The method is based on retention of volatile phenols on adsorbants. Among the tested resins, the most efficient, AG 2-X8 (anion exchange resin), worked as well with a synthetic solution as with wines. The percolation of clarified wine adjusted to pH 9 on this resin permits, in particular, the elimination of organic acids. Phenols are not eluted after rinsing the column with 1N HCl, but are eluted with methanol after this treatment. Good recovery (91 %) and good repeatability are observed. The eluate is directly analysed by HPLC on an RP18 column after two-fold dilution in water. The four volatile phenols were completely separated and detected by UV at 280 nm with high sensitivity (20–40 ppb). No interference with other compounds were noted in the different wines analysed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 30
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 61-66 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Sugars ; Ion-pair separation ; Pulsed electrochemical detection ; Hypercarb and PLRP-S
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Sugars, sugar acids, amino sugars and oligomers are separated as ion-pairs with hydrophobic counter ions at alkaline pH using PLRP-S and Hypercarb as solid phases. Important parameters for regulation of retention and selectivity are nature and concentration of the counter ion, pH (hydroxide concentration) and temperature. Reversals in the elution order wer obtained in some cases. Oligosaccharides are highly retained in these systems. The addition of organic modifiers to the mobile phase for elution of the solutes were found to interfere with the pulsed electrochemical detection (PED). Anions added to the mobile phase compete with the solutes for ion-pair retention, hence, decreasing the capacity factors, and phosphate could be used for this purpose in the separation of maltooligomers (M2-M10) from corn syrup.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 31
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 55-60 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbohydrates ; Ion-pair separation ; Glycoconjugates ; PRP-1 and PLRP-S
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A new technique for the separation of carbohydrates as ion-pairs in strongly alkaline solution is presented. Carbohydrates are weakly acidic and partly present as anions at pH≥12 [1]. They are retained as ion-pairs on polymeric solid phases (PRP-1 and PLRP-S) with a hydrophobic quaternary ammonium counter ion present in the mobile phase. The effects of nature and concentration of mobile phase components on the retention of carbohydrates have been investigated and an ion-pair distribution model is proposed. The influence of temperature indicated no changes in retention mechanism with high counter ion concentration, but the resolution decreased with increasing temperature. Saccharides added to the mobile phase were shown to increase the retention and the selectivity.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 32
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 90-92 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; α1-acid glycoprotein ; Complex glycans ; Allergic reaction ; Terfenadine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Little is known about the alterations that have occurred at the individual glycosylation sites in allergy patients or how these glycosylation patterns may change after anti-allergy treatments. Using reverse-phase HPLC, we have quantitated the glycoforms present at the individual glycosylation sites on orosomucoid isolated from the sera of allergic reaction patients and an allergic reaction patient treated with the antihistamine Terfenadine. The glycan structures isolated from the five glycosylation sites for the individual taking Terfenadine were all within normal ranges. It is suggested that if the changes in glycosylation in OMD in the allergic state are functionally driven, then it should be possible to correlate biological activities with quantitative changes at the individual glycosylation sites, and hence further define the role of OMD in allergy and inflammation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 33
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Bonded liquid crystals ; Polymeric stationary phases ; Variable temperature studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Using two polycyclyic aromatic hydrocarbons as solutes, a comparison is made between a bonded liquid crystal stationary phase and a conventional polymeric C-18 phase. The bonded nematic liquid crystal phase was the silanized form of 4-[4-(allyloxy)benzoyl-oxy]biphenyl and the polymeric phase was Vydac 201TP. Both phases display shape and planarity selectivity as indicated by the results of the variable temperature and mobile phase composition studies. The slot theory of retention can be used to explain these results. However, the liquid crystal phase is more sensitive to molecular geometry, probably due to its more ordered structure on the surface. Variable temperature experiments which compare retention during both heating and cooling provides additional support for this conclusion. With the polymeric bonded C-18 phase, each solute had identical retention at the same temperature during both the heating and cooling cycles. On the bonded liquid crystal phase, measurable differences in retention were observed at identical temperatures depending on whether the column was heated or cooled. This effect is attributed to a degree of partially reversible disordering which occurs as the column temperature was increased. However, conditioning with the appropriate mobile phase can restore the original retention characteristics of the bonded liquid crystal phase.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 34
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 133-138 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ligand exchange chromatography ; Zirconium oxide ; Aluminum oxide ; Lewis acids and bases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The chromatographic properties of alumina and zirconia are compared with respect to their relative Lewis acidities when parabenzoic acid derivatives are chromatographed in aqueous media. Weak Bronsted acids show similar capacity factors on alumina and zirconia. As solute Bronsted acidity increases, the capacity factor increases more significantly on the zirconia phase than on the alumina phase. Efficiencies, as determined by the dominant desorption rate constants, are generally comparable between the two phases with alumina showing slightly higher efficiencies, Secondary interactions between the solutes and the stationary phases are nearly absent on zirconia but are very apparent on the alumina phase.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 35
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Formaldehyde in air ; Fluorimetric detection ; Comparison of fluorescent derivatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Three different fluorimetric methods for the measurement of atmospheric formaldehyde are described. The relatively unknown 2-diphenylacetylindan-1,3-dionhydrazine (DAIH) method was developed and optimized with an improved limit of detection of 2 ppbv corresponding to a sample volume of 7.5 L. Separation of lutidines necessary for the Nash method was successfully carried out and the separation time was reduced by a factor of 5. The detection limit of the Nash method was improved to 1 ppbv. In comparison, the N,N-diphenyl-1-naphthylamine-5-sulfonhydrazine (DNSH) method performed in a similar manner, reached an LOD of 0.5 ppbv. Finally, measurements made in candle smoke using the three different fluorimetric methods and three standard methods yielded very similar results.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 36
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; 18 β-glycyrrhetinic acid ; Glycyrrhizinic acid ; Toothpastes ; Solid phase extraction (SPE)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary HPLC methods suitable for the selective and sensitive determination of 18 β-Glycyrrhetinic acid (GT) and Glycyrrhizinic acid (GZ) in toothpastes have been developed. The methods involve a preliminary quantitative solid phase extraction (C-18 sorbent) for sample clean-up and analyte concentration. Chromatographic separations were performed on reversed phase (C-8; C-18) columns using mixtures of methanol and phosphate buffers (pH 3.0) as the mobile phase under isocratic or gradient elution conditions. The method were applied successfully to the analysis of commercial toothpastes containing low levels (0.013–0.065%) of GT and GZ.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 37
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Amphetamine in plasma ; Enantiomeric separation ; Solid phase derivatization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An analytical approach has been developed for the one step determination of enantiomeric amphetamine composition in plasma, using on-line, pre-column solid phase derivatization with reversed phase HPLC separation. The high molecular weight protein components were excluded by the small pore structure of the polymer and washed out of the reaction column before derivatization. Spiked amphetamine in human plasma was extracted and derivatized by the polystyrene based FMOC-L-prolyl solid phase reagent. The derivatized diastereomers were separated on a conventional ODS column with an ACN/H2O mobile phase. No kinetic resolution or racemization was observed in this solid phase derivatization. Calibration plots and reproducibility experiments were performed to demonstrate the validity of the new approach. Automation of the procedure provided a simple and reproducible method for direct chiral recognition in plasma samples.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 38
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Pre-column derivatisation ; Morpholine and degradation products ; Boiler feed water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Morpholine and its amine-type thermal degradation products present in boiler feed water and steam condensate were derivatised with N-succinimidyl-p-nitrophenylacetate. These pre-column derivatives were determined by high-performance liquid chromatography with UV detection at 280 nm. The analytical column was Supelco-sil-ODS with an isocratic mobile phase. Morpholine and its breakdown products were monitored in the range 0.01–10 μg ml−1 with a relative standard deviation of 0.4–3.0%. Chromatographic analysis of boiler feed water and steam condensate samples collected from a boiler servicing a petroleum refinery is described.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 39
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 189-192 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carotenoid pigments ; Chlorophylls ; Photosynthestic tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A high performance liquid chromatography system for the analysis of photosynthetic pigments is presented. The method employs an octadecylsilica stationary phase, a programmed quaternary mobile phase consisting of mixtures of methanol, acetonitrile, water and hexane, and a photodiode array detector. Carotenoids and chlorophylls are rapidly analysed in a single chromatographic separation. Thecis-trans isomers of most carotenoids are separated by this method.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 40
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Monensins A and B ; Fermentation broth ; Streptomyces cinnamonensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary High performance liquid chromatography on an octadecyl silica column has been used to determine both the monensin A: monensin B ratio and by the method of standard addition, the concentration of both in the fermentation broth ofStreptomyces cinnamonensis. Refractive index detection was preferred to ultraviolet owing to the presence of UV-absorbing components which could not be completely separated from the substances of interest. A linear relationship was obtained from the calibration data. The coefficients of variation for the estimation both of the ratio and the concentrations of the compounds were better then 5%. The estimated limit of detection for both substances was about 1 μg/ml. The results obtained from the determination of the ratios of monensins were compared with those obtained by chemical ionization mass spectrometry. Chromatographic separation of monensins on the silica gel column is also described.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 41
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ascorbic acid ; Antioxidant synergists ; Food, pharmaceuticals and cosmetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A specific and sensitive reverse-phase HPLC method for the quantitative determination of ascorbic acid and antioxidant synergists (1-tartaric acid, citric acid, lactic acid as lithium lactate and EDTA) in fatty pharmaceuticals, cosmetics and food has been developed. Two extraction procedures were used; treatment with hot water, and extraction with water from a hexane dilution of the product. No significant differences between the two procedures were found (p〈0.05), except for ascorbic acid. Quantitative determinations were performed using a C-18 column and sulfuric acid (pH 1.95) mobile phase. With detection, at 210 nm, lactic acid overlapped with ascorbic acid, but the former could be readily identified by TLC. Ascorbic acid was detected at 254 nm, when lactic acid (as lithium lactate) did not interfere in the analysis. Mean recoveries for tartaric, citric and lactic acids were in the range 96–101%.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 42
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Microdialysis ; Fermentation monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An automated on-line sampling and analytical set-up for the control of fermentations was studied incorporating a microdialysis probe as the sampling device. Applications to a penicillin broth and an ethanol fermentation were studied. Typical recovery values of carbohydrates were found to be close to 100% even after exposure of the microdialysis probe in the process for about 30 h.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 43
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 264-268 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Enantiomer separation ; Stereospecific synthesis ; Crown ether phase ; Immobilized enzyme reactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A prototype of a coupled-column chromatographic system has been developed to determine the enantiomeric purity of enzymatically produced chiral compounds. In this system, an immobilized enzyme HPLC reactor based upon α-chymotrypsin (ACHT-IMER) was linked through a switching valve to a chromatographic column containing a crown ether-based chiral stationary phase (CR-CSP). Although ACHT is normally stereospecific for the L-enantiomorphs of aromatic amino acids, L-tryptophan (L-TRP), L-tyrosine and L-phenylalanine, we were able to achieve the hydrolysis of D-TRP methyl and ethyl esters on the ACHT-IMER. The percent of hydrolysis of the D-TRP esters could be manipulated by varying the resident time in the ACHT-IMER. In a series of experiments, D,L-TRP methyl or ethyl esters were injected into the ACHT-IMER, the flow stopped and after resuming the flow, the eluent which contained the hydrolyzed aminoacids, D- and L-TRP, was switched onto the CR-CSP where the enantiomeric composition of the TRP peak was determined. This configuration is a model for other IMER/CSP coupled-column systems which can be used for analytical and preparative applications.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 44
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 275-280 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Thin-layer chromatography ; Anti-hypoxia drugs ; Lipophilic character and retention
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The lipophilicity of 14 anti-hypoxia drugs has been determined by reversed phase thin-layer (RPTLC) and reversed phase high performance liquid chromatography (RPHPLC) in eluent systems containing different concentrations of acetonitrile and potassium dihydrogen phosphate. There was significant correlation between lipophilicity and the specific hydrophobic surface area of the drugs in RPTLC, indicating that the drugs behave as an homologous series of compounds. In RPTLC the concentration of buffer has a negligible effect on the retention of the drugs whereas in RPHPLC the buffer concentration influenced the retention. This discrepancy can be explained by the lower sensitivity of RPTLC. There was strong correlation between lipophilicity values determined by both methods, proving that both are suitable for the determination of molecular lipophilicity.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 45
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; 1,4-Benzoxazin-3-ones ; Cyclic hydroxamic acids ; β-Cyclodextrin modified silica gel ; Enantiomer separation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A liquid chromatographic procedure on a 5 μm LiChroCART column using chemically bonded β-cyclodextrin (ChiraDex) as chiral selector was developed for the enantiomeric separation of four 2H-1,4-benzoxazin-3(4H)-ones which are close analogs of the cyclic hydroxamic acids 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA) and 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3 (4H)-one (DIMBOA) naturally occurring inGramineae species.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 46
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 295-301 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Used column packings ; Particle size distribution ; Scanning electron microscopy ; Column age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Particle size distribution analysis and scanning electron microscopy (SEM) were carried out on eight used HPLC columns containing either irregular silica based, spherical silica based or spherical polymer based packing material. Particle size distributions of the used irregular silica based columns were at least bimodat at the outlet ends and either biomodal or log-normal at the inlet ends with regular progressions between the two extremes through the column. A new ODS-3 column showed log-normal size distributions from the inlet to the outlet ends. Spherical silica based column particle size distributions showed distinct shoulders on large central distribution peaks in most column sections with various degrees of shoulder erosion. The spherical resin based column showed a broader inlet particle size distribution progressing to a very narrow outlet distribution. SEMs of both irregular and spherical silica based columns revealed a larger number of undersized particles and debris at the outlet than inlet ends which could have resulted from stationary phase degradation, since this was not seen in the new ODS-3 column. While several SEMs of the spherical silica based columns revealed hollow spheres and twins, the spherical resin based column packing showed stress fractures or wrinkle lines resulting from use or dehydration.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 47
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 311-316 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Isolation of natural products ; Solid phase extraction ; Chemically bonded phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Esculin (ESC) and rutin (RUT) have been simultaneously isolated from pharmaceutical natural materials by solid phase extraction (SPE). Determination of both substances was performed by reversed phase high performance liquid chromatography (RPHPLC) with UV detection. Optimization of the separation conditions showed that simultaneous isolation and determination of rutin and esculin from pharmaceutical material was possible. The recovery obtained was not lower than 95±2%.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 48
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Enantiomer separation ; Chiral ovomucoid column ; Diltiazem hydrochloride ; Clentiazem maleate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A direct HPLC separation method was developed for the determination of the enantiomers of racemic precursors to diltiazem (I) and its 8-chloro derivatives (II). The enantiomers were successfully separated on a chiral ovomucoid column using an aqueous-organic mobile phase (reversed-phase HPLC). The influence of the organic modifier and buffer pH on the retention and enantioselectivity was investigated. The chromatographic conditions chosen for the separation permitted complete resolution of the enantiomers of both the acid (Ib and IIb) and methyl ester precursors (Ia and IIa) within 20 min. The influence of sample load on retention times, theoretical plates numbers, peak heights and peak areas was also investigated. The peak areas showed a good linearity over the concentration range examined, although all the others were influenced significantly by the sample size. An optical antipode of the intermediate to be determined could be detected by the area-percentage method down to ca. 0.1%, together with the determination of its precursor, including its optical purity.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 49
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Probenecid in urine ; Solid-phase extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary This study describes a rapid method for the determination of probenecid in human urine by liquid chromatography with UV detection at 254 nm, after clean-up through a C8 solid-phase extraction column. Liquid chromatography was carried out on a C18-bonded phase using an acetonitrile-acetate buffer (pH=4) gradient elution. Ethacrynic acid was used as internal standard. The system has been applied to the determination of probenecid in the 0.10–100.0 μg/ml concentration range; the limit of detection was 5 ng/mL.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 50
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 390-394 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Electrostatic model ; Ion-pair reagent ; Organic modifier ; Prediction of k′ values
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The capacity factors of phenylamine and naphthylamine sulphonic acids in reversed-phase, ion-pair liquid chromatography (RP-IPC) were measured. The combined effects of organic modifier (Cb) and ion-pair reagent concentration (Cp) on retention follow an equation based on the electrostatic model: $$\ln k' = a + b lnC_p + cC_b $$ the experimental value of b correlates well with the value of 0.5 z (z=charge on analyte ion) predicted for mono and divalent ions. The measured value of b is, however, lower than that predicted for trivalent ions, which may be due to the effective number of charges being less than the apparent number. The absolute values of a and c are much larger than those in RP-HPLC in the absence of an ion-pair reagent, quantitative correlation of a and c with retention values in RP-HPLC and solute charges has been observed and a good linear relationship between a and c has been obtained, strongly supporting the validity of the electrostatic retention model. A critical value, R, at which the negative effect of methanol on retention is equal to the positive effect of the ion-pair reagent (TBAI) has been proposed. The critical values obtained are related to the behaviour of the solute, the ion-pair reagent and the stationary and mobile phases, and lies between −0.04 and −0.065 in most cases, which means that the increase by exp times (=2.718×) the ion-pair concentration the original is equivalent to a decrease of 0.04–0.065 in volume fraction of methanol
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 51
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 381-386 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Bovine trypsin ; Porcine kallikrein ; Bovine pancreatic trypsin inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The possibility of determining more than one enzyme at the same time has been examined. A new approach, based on the measurement of a direct and specific chromatographic signal obtained by hydrophobic interaction chromatography (HIC) of the stable complex formed with the inhibitor aprotinin, is proposed. A basic procedure for the quantitative determination of trypsin and kallikrein, taken as models, is described. The method is precise with a mean coefficient of variation of 3.1% and 3.5% for trypsin and kallikrein, respectively; the limit of determination for both enzymes is 0.17 nmol ml−1 in the original sample.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 52
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 387-389 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Buffer composition effect ; Agarose ; Albumin ; Ionic adsorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The adsorption of albumin on a commercial, high quality agarose (Sepharose® 6B) was studied in different buffers of low pH (4.00) and low molarity (9–11 mM). Eluents containing 0.025% albumin were introduced onto small gel columns until saturation and the adsorption capacity of the gel in different buffers calculated. Adsorption was high in solutions of monobasic acids. Although cation-exchange interactions between the protein and the gel matrix were found to be influenced essentially by the sodium ion concentration or ionic strength of the buffer, adsorption in cirate buffers is weak compared to that in formate, acetate or propionate buffers of about the same ionic strength (I=0.01).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 53
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Polyunsaturated fatty acids ; Direct injection ; Enthalpy of adsorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Retention studies on arachidonic acid 5, 8, 11, 14 all cis eicosatetraenoic acid, C20:4ω6), eicosapentaenoic acid (5, 8, 11, 14, 17 all cis eicosapentaenoic acid, C20∶5ω3) and docosahexaenoic acid (4, 7, 10, 13, 16, 19 all cis docosahexaenoic acid C22∶6ω3) were performed on a commercial μBondapak free fatty acid analysis column. The ternary mobile phase consisting of acetonitrile, water and tetrahydrofuran was used in an isocratic mode with differential refractometry detection. Retention data were mesured at various flow rates with two different, mobile-phase compositions. Capacity factors and enthalpy of adsorption were calculated from the retention data. Finally the retention mechanism is explained.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 54
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 403-409 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed phase packings ; Polybutadiene coating ; Polymer coatings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Non-wetting viscous liquids such as oligobutadiene prefer “active” sites such as pores during the process of physisorption. Thus, polybutadiene (PBD) coatings on porous silica do not result in a homogeneous polymer film but in an inhomogeneous loading where the bulk polymer is mainly sited in the pores of the silica. An increasing polymer loading leads to increasingly filled pores instead of a thicker polymer film. We cannot exclude the possibility that most of the surface is covered at least with a thin polymer film since the chromatographic behaviour is relatively good for polypeptides, which are highly susceptible to the silanol groups of silica.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 55
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 435-438 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Pirarubicin and metabolites ; Anthracyclines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A new and highly sensitive HPLC method for the simultaneous determination of pirarubicine (THP-doxorubicin) and its metabolites, adriamycin and adriamycinol, in human plasma, is described. Samples were treated by liquid-liquid extraction, the organic phase removed and the residue dissolved in methanol. Separation was on a Lichrocart Supersher RP 8 column, (250×4 mm) 4 μm, with a mobile phase of acetonitrile/methanol/formate-buffer.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 56
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 439-447 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Affinity chromatography ; Polythiolpeptides and metallopolythiolpeptides ; Mechanism of separation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Covalent affinity chromatography with thiol-disulphide interchange (CAD-TDI) is a method for the separation of thiolproteins based on the interaction between disulphide bridges immobilised on a insoluble support and thiol groups in the protein undergoing separation. Mercury-thionein, cadmium-thionein and apothionein have been used as low molcular weight thiolproteins rich in cysteine residue. The proposed separation mechanism is more complex than those in the literature and can have anionic, cationic or a free radical character depending on the protein, the ligand and the conditions during separation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 57
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; UV and electrochemical detectors ; Phenolic antioxidants ; Migration studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The performance of UV diode-array, spectrometric and electrochemical detectors was compared in the chromatographic analysis of trace amounts of six phenolic antioxidants. Quantitative validation was undertaken; the linearity range was wider using UV detection although the limits of detection were lower with electrochemical detection. UV detection was applied both to identification of an antioxidant in a hydrophilic suspension and to a migration study.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 58
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Pattern recognition ; Principal components ; Multiple linear regressions ; M.O. properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The use of theoretically calculated molecular properties as predictors for retention in reversed-phase HPLC has been explored. HPLC retention times have been measured for a series of 47 substituted aromatic molecules in three solvent mixtures and steric and electronic properties of these compounds have been derived using semi-empirical molecular orbital and empirical theoretical methods. A subset of the experimental data (a training set) was used to derive property-retention time relationships and the remaining data were then used to test the predictive capability of the methods. Good retention time prediction was possible using derived regression equations for individual solvents and after including solvent parameters it was possible to predict retention for all solvents using a single equation. This method showed that the most useful properties were calculated log P and the calculated dipole moment of the solutes, and the calculated solvent polarisability. In addition, 90% of the data were used to train an artificial neural network and the remaining 10% of the data used to test the network; excellent prediction was obtained, the neural network approach being as successful as the regression analysis.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 59
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 37 (1993), S. 284-286 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Composition of chelates ; 3,5-diBr-PADAP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The composition of 3,5-diBr-PADAP metal chelates was determined by liquid chromatography employing appropriate eluents and non-polar bonded stationary phase. The metal-to-ligand ratios were 1∶2, 1∶2 and 1∶2 for Cu(II), Co(III) and Cr(III) respectively, and the V(V)-to-ligand ratio found to be 1∶1∶1 in V(V)-3,5-diBr-PADAP-H2O2 in the presence of H2O2. The results are in agreement with literature data.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 60
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 37 (1993), S. 492-496 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chlorine in air and water ; Precolumn derivation ; 4-Bromoacetanilide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Chlorine has been be determined in air and water samples by a rapid and sensitive method entailing precolumn derivatization to 4-bromoacetanilide. A mixed potassium bromide-acetanilide reagent was used as a trapping agent for chlorine in air, and for its derivatization. The 4-bromoacetanilide formed was determined by reversed-phase HPLC on an ODS column, using methanol-water, 65∶35 (v/v) as mobile phase; detection was at 240 nm. A rectilinear calibration graph was obtained for the range 0.1–30 μg mL−1 chlorine; the limit of detection found to be 0.01 μg mL−1. The precolumn derivative has been found to have a shelf-life of at least 21 days; this enables the use of the method for samples transported from the field to the analytical laboratory, or the testing of a variety of conditions for chlorine scrubbing studies without the need for immediate analysis of samples. Humic substances do not cause any interference with the proposed method and the presence of nitrite does not lead to artificially high results and consequent misleading conclusions of the presence of high levels of chlorine.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 61
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 37 (1993), S. 549-561 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Stationary phases ; Polymer-coated silica ; Polymer-filled silica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A variety of stationary phases for HPLC can be prepared by immobilization of organic polymers on silica gels. Such silica-polymer adsorbents combine the strength of an inorganic matrix with the selectivity and chemical inertness of organic resins. Moreover, effective shielding of residual silanols and the increase of column packing stability can also be achieved by modification of silica gels with organic polymers. There are two main approaches to the preparation of such composite sorbents. The first includes various routes for coating the silica surface with a polymer layer and the second is by filling the silica pores with a polymer network. A survey of polymers immobilized on silica gels and of various experimental procedures which can be used for composite sorbent synthesis is given in this review.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 62
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied phycology 5 (1993), S. 175-181 
    ISSN: 1573-5176
    Keywords: decomposition ; Gracilaria ; nitrogen ; nutrients ; temperature ; Ulva
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study determined the rate at which nitrogen accumulated in seaweeds is released during decomposition and the effect of temperature on their rates of decomposition and nitrogen release. Gracilaria verrucosa and Ulva lactuca decomposed rapidly in outdoor mesocosms. Ulva, but not Gracilaria, became nitrogen-enriched during decomposition. Maximal weekly rates of nitrogen release were 5.91 ± 2.23 and 6.37 ± 2.59 g N m−2 d−1, respectively for Gracilaria and Ulva. Temperature had a significant effect on the decomposition rate of Gracilaria in a laboratory experiment: decomposition was greater at 30 °C than at 25 °C. No net decomposition was observed at 16 °C. Gracilaria became nitrogen enriched at 30 °C, but not at 16° or 25°. The release of stored nutrients from decaying seaweeds should be included in nutrient budgets and models when seaweed standing stocks are significant. Seaweed source-sink relationships are important ecologically and can be applied to attempts at using seaweeds as environmental monitors of anthropogenic eutrophication and to efforts of cultivating seaweeds for the improvement of water quality.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 63
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied phycology 5 (1993), S. 547-549 
    ISSN: 1573-5176
    Keywords: Trentepohlia odorata ; Dunaliella bardawil ; light intensity ; nitrogen ; growth ; carotenogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract AxenicTrentepohlia odorata was cultured at three different NH4Cl levels (3.5 × 10−2, 3.5 × 10−3, 3.5 × 10−4 M) and three different light intensities (48, 76, 122 µmol m−2 s−1). Chloride had no effect on growth over this range of concentration. High light intensity and high NH4Cl concentration enhanced the specific growth rate. The carotenoid content increased under a combination of high light intensity and low N concentration. WhenD. bardawil was exposed to the same combination of growth conditions, there was an increase in its carotenoid content. The light saturation and the light inhibition constants (K s andK i, respectively) for growth, and the saturation constant (K m) for NH4Cl were determined. TheK s andK i values were higher inT. odorata (66.7 and〉 122 σmol m−2 s−1, respectively) than inD. bardawil (5.1 and 14.7 µmol m−2 s−1, respectively). TheK m value determined at 122 µmol m−2 s−1, however, was lower inT. odorata (0.048 µM) than inD. bardawil (0.062 µM).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 64
    ISSN: 1573-5176
    Keywords: agar ; C:N ratio ; chemostat ; Gelidium ; nitrogen ; phycobiliproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gelidium sesquipedale is the most important raw material used for extraction of agar in Spain. Based on chemostats, a system of culture for macroalgae with a continuous flow of culture medium has been developed. A stressed morphotype from the South of Spain was cultured, and the effects of different rates of NO 3 − flow on growth and internal constituents were investigated in the laboratory. Cultivation was successful after optimizing factors affecting growth, such as irradiance level, renewal rate and water movement. Mass production was dependent on N supply. With a flow of 35 μmol NO3 − g−1 DW d−1, optimal values of growth (2.1% d−1) and biomass yield were obtained. In these conditions, biomass yield resembled the values observed in natural populations (about 500 g DW m−2 y−1). When the flow of N was reduced to 15 μmol NO 3 − g−1 DW d−1, growth rate and biomass yield were reduced three-fold, and were null when N was supplied as 7 μmol NO 3 − g−1 DW d−1. C:N ratio was an index of the physiological status of the tissue, remaining low when N was sufficient and raised to critical values when N supply was limited. Phycobiliproteins, kept at a constant irradiance level, were affected by N supply, acting as an internal nitrogen reserve, unlike chlorophylla. An effective phycobiliprotein synthesis took place when the flow of N was sufficient. Agar yield, on dry weight basis, was similar as a function of N flow, whereas agar yield of the culture was higher when N was sufficient as a result of growth not being limited by N. This system of culture, commonly used in microalgal studies, may have an important use in macroalgae as a system to obtain biomass of high quality as well as a good tool for physiological studies in conditions of continuous and controlled flow of nutrients.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 65
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 152 (1993), S. 19-23 
    ISSN: 1573-5036
    Keywords: acetylene reduction ; difference method ; legumes ; 15N isotope method ; nitrogen ; nitrogen fixation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To assure proper management and fully realize the benefits of the legume-Rhizobium symbiosis it is necessary to be able to quantify the amount of nitrogen fixed. Having measured the effectiveness of atmospheric N2 fixation the macro- or micro-symbionts as well as agronomic factors can be manipulated with the objective to maximize biological nitrogen fixation. A suitable method to quantify nitrogen fixation is therefore necessary in any programme aiming at increasing N2 fixation, like the one being reported in this volume. There are several methods available to quantify nitrogen fixation and most of the commonly used ones are described in the present paper listing their advantages and disadvantages.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 66
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 155-156 (1993), S. 57-66 
    ISSN: 1573-5036
    Keywords: nitrogen ; wheat ; simulation ; yield-response curve
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Models of the yield responses of crops to applied nutrients are a recent addition to the methods available for making fertilizer recommendations. They have a place in integrating nutrient information with information on other factors which affect yield and its response to added nutrients. This review deals with nitrogen models classified into three groups: those which predict yield-response curves based on empirical factors; those which simulate the yield response from complex simulation models of many processes regulating crop growth and the soil environment; and those which aim to simulate yield and selected processes based on simplified functional relationships which apply to a target region or industry. Three case studies representing the three classes of model are drawn from research on dryland wheat in different parts of Australia. They show examples in which models provide information which is unobtainable from experimental procedures and which provide information useful to farmers in making decisions about fertilizers. Suggestions are made for future developments in crop-nutrient modelling including further comparisons of models, linkage of models with tissue tests, modelling co-limiting nutrients, deciding on the appropriate level of detail within a model and the need for methods for calibrating and testing models on attributes other than yield alone.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 67
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 157 (1993), S. 167-174 
    ISSN: 1573-5036
    Keywords: extraction ; mineralisation ; nitrogen ; potassium chloride ; uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The mineral nitrogen extractable from 12 Scottish arable soils by boiling for 1 hour with 1 M KCl solution was compared with the quantities taken up by ryegrass, barley and oats in pot tests. High correlations were obtained for ryegrass and oats (r2=0.85 and 0.79, respectively), with a slightly lower correlation (r2=0.64) for barley. When the results for one anomalous soil were eliminated from the analysis, all r2 values were 0.81 or higher. The results confirmed earlier work indicating that this method may prove useful for predicting crop uptake of soil-derived nitrogen.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 68
    ISSN: 1573-5036
    Keywords: fructans ; NIR ; nitrogen ; non-structural carbohydrates ; rice ; starch ; stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant shoot samples are frequently analysed to assess if crops require additional nitrogen or mineral elements to maintain satisfactory growth. If plant growth is limited by temperature, water stress, disease, lodging or a mineral deficiency, non-structural carbohydrates (NSC) may be accumulated in, or depleted from, tissues especially those in the lower stems. Plant testing laboratories do not routinely analyse NSC to assist in the identification of plant stress probably because skilled technicians and time are required for the wet chemical determination. In this paper we report that routine determination of NSC is possible using near-infrared reflectance spectroscopy; the errors of determination are comparable with traditional chemical methods. The concentration of NSC in the shoots of rice grown in south eastern Australia ranges from 1.6 to 22.8%, as starch. In the shoots of wheat grown in eastern Australia the range is from 2.4 to 35.2%, as fructans. In both crops the NSC content is highly inversely correlated with the shoot nitrogen content. Based on data from commercial wheat and rice crops we suggest that the ratio between nitrogen and NSC can be used to identify crops in which growth has been limited by a stress other than nitrogen and so are unlikely to show the predicted response to an application of nitrogen fertilizer.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 69
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 155-156 (1993), S. 45-55 
    ISSN: 1573-5036
    Keywords: compartmentation ; cytoplasm ; 39K NMR ; maize ; nitrogen ; 14N NMR ; 15N NMR ; pea ; phosphorus ; potassium ; 31P NMR ; vacuole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The cytoplasmic and vacuolar pools of ammonium, inorganic phosphate and potassium can be studied non-invasively in plant tissues using high resolution nuclear magnetic resonance spectroscopy. The techniques that allow these pools to be discriminated in vivo are described and their application to plants is reviewed with reference to the phosphorus, nitrogen and potassium nutrition of root tissues.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 70
    ISSN: 1573-5036
    Keywords: amino acid ; nitrogen ; phloem sap ; phosphorylation ; protein ; rice (Oryza sativa L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pure phloem sap was collected from insects feeding on rice (Oryza sativa L.) leaves by a laser technique similar to the aphid stylet technique. Rapid circulation of nitrogen in the sieve tubes was demonstrated directly using 15N as a tracer. Application to the roots of the metabolic inhibitors of amino acids, aminooxyacetate and methioninesulfoximine, changed the amino acid composition in the sieve tubes. Feeding methionine to leaf tips resulted in its bulk transfer into the sieve tubes. In vitro experiments confirmed the existence of protein kinases in the pure rice phloem sap. The phosphorylation status of the sieve tube sap proteins was affected by the light regime. The possibility that changes in chemical composition or protein modification such as phosphorylation in the sieve tubes might affect plant growth are discussed. Analysis of pure phloem sap collected from rice plants by insect laser technique has shown dynamic changes in the chemical composition and the quality of proteins in the sap.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 71
    ISSN: 1573-5036
    Keywords: critical concentration ; magnesium ; manganese ; nitrogen ; phosphorus ; potassium ; solution culture ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Solution culture was used to characterise deficiencies or toxicities of several essential elements in Ipomoea batatas cv. Wanmun, and to define the critical concentrations of these elements in young mature leaves during vegetative growth. Tentative critical concentrations for deficiency, expressed on the basis of dry weight of leaf blade, were: nitrogen 3.8%, phosphorus 0.17%, potassium 2.4%, magnesium 0.12%, manganese 20μg/g and zinc 10μg/g. For manganese and zinc toxicities it was possible only to designate the range within which the critical concentration occurred. Visible symptoms are briefly described.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 72
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 155-156 (1993), S. 21-31 
    ISSN: 1573-5036
    Keywords: nitrogen ; sulphur-nutrition ; gene cloning ; gene expression ; regulation ; crop improvement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the last decade, understanding of ion transport has grown sufficiently to pose sensible questions about the molecular nature of the processes and their regulation. Techniques for identifying and cloning genes and for genetic transformation provide the means for answering these questions. Transport of ions across membranes is obviously a major aspect of mineral nutrition since it occurs during initial absorption, compartmentation and mobilisation of nutrients. Here, we will briefly review the types of transport protein involved and show how molecular biology and recombinant DNA technology have revealed something of their structure. Strategies used to identify the genes for transporters are discussed and reference is made to areas in which the availability of cloned genes will facilitate future studies. Mineral nutrition involves, however, more than membrane transport. The absorption rates of major nutrients are quite strictly regulated by biochemical factors which vary with the rate at which nutrients are used in growth. Nitrogen, sulphur and phosphate nutrition in micro-organisms are regulated by the interaction of various DNA-binding proteins with the promoter regions of genes for key enzymes in the assimilatory pathways and the specific ion permeases. The expression of the regulatory protein or its activity can be modified by metabolites, such as glutamine. Some evidence supports the idea that higher plants also have groups of genes with a common regulation of expression. An attempt is made to identify some reasonable objectives, which should increase understanding of the regulation of nutrient transport.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 73
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 152 (1993), S. 255-260 
    ISSN: 1573-5036
    Keywords: crimson clover ; field labeling ; legume ; nitrogen ; 15N ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant material labeled with 15N is often used to determine recovery of N from green manure crops by subsequent crops. In this study, 15N enriched crimson clover (Trifolium incarnatum L.) was grown at a field site where it was to be utilized in a subsequent experiment. A foliar spray of (NH4)2SO4 (99 atom % excess 15N) was applied to a 1.2 m × 8.8 m plot of crimson clover at a rate of 10 kg N ha−1 in early March 1990, immediately prior to the period of rapid vegetative growth. Clover shoots harvested in April contained 1.72 atom % excess 15N. Total N concentration of enriched clover was similar to that in adjacent untreated clover. Clover shoots contained 20% of the applied 15N, and an additional 27% was recovered from the surface soil horizon (0 to 15 cm). A gradient was observed across the plot, with clover enrichment increasing from 1.3 to 2.2 atom % excess 15N. Recovery of applied 15N in soil was highest in the subplots with lowest clover enrichment. Variability in 15N enrichment was also observed among plant parts: leaves from the basal half of shoots had 2.2 atom % excess 15N; while leaves from the terminal half of shoots, terminal stems, and basal stems had between 1.1 and 1.4 atom % excess 15N.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 74
    ISSN: 1573-5036
    Keywords: allocation ; carbon ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In this paper we model allocation of carbon and nitrogen to roots and leaves as a function of the nitrogen status of a plant. Under steady-state conditions, allocation of carbon and nitrogen to leaves is exponentially (positively) correlated with plant nitrogen concentration, whereas allocation to roots is correlated negatively, also in an exponential manner. Allocation functions derived under steady-state conditions are used to simulate biomass partitioning under non-steady-state nutrient conditions. Upon nitrogen deprivation, measured and simulated values are rather similar with time, suggesting that allocation functions derived under steady-state conditions also hold under non-steady-state conditions.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 75
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 155-156 (1993), S. 387-390 
    ISSN: 1573-5036
    Keywords: Brassica napus L. ; canola ; critical concentrations ; nitrate ; nitrogen ; sowing time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Critical concentrations of NO3-N in fresh petiole tissue and total N in the dried lamina were determined for the youngest mature leaf (YML) of field-grown canola. For dry matter yield of canola sown on 4 May, critical NO3-N concentration in the YML petiole at the rosette stage (RS) was 1.46 mg/g fresh wt. At the flower-buds-visible stage (BV) it was 0.45 mg/g fresh wt. For seed yield the values were 1.72 and 0.53 mg/g fresh wt. Critical total N concentration in the YML lamina for dry matter yield were 69 mg/g dry wt. at RS and 57 at BV. For seed yield they were 71 and 59 mg/g dry wt. Critical NO3-N concentrations in the YML petiole of canola sown on 30 May were reduced by 50%; critical total-N concentrations in the YML lamina were not reduced to the same extent. Despite the reductions in critical N concentrations in the YML, critical N fertilizer rates for vegetative growth and seed yield were unaffected by sowing date or plant growth stage.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 76
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 153 (1993), S. 255-263 
    ISSN: 1573-5036
    Keywords: acid soil ; chlorosis ; iron deficiency ; nitrogen ; Pinus sylvestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Scots pine (Pinus sylvestris L.) trees growing on acidic soils may show a special type of chlorosis. The current needles are yellowish and the discolouration is most pronounced at the needle base. The disease bears resemblance to iron chlorosis in trees on calcareous soils. In order to investigate the cause of the chlorosis, needle and soil samples were collected in 41 stands in the southern, central and eastern parts of the Netherlands. Needles of the chlorotic trees had low levels of chlorophylls and carotenoids and high ratios of carotenoids to chlorophylls and of chlorophyll a to chlorophyll b. These symptoms occur in plants suffering from iron deficiency. The current needles of chlorotic trees contained on average 14 mg kg−1 Fe, whereas unaffected trees from the same stands contained 24 mg kg−1. Healthy trees from stands without chlorotic trees had needle iron levels of 29 mg kg−1. Spraying the needles of chlorotic trees with ferric-EDTA containing solutions resulted in partly regreening within a few weeks. Chlorotic needles showed also high nitrogen and cadmium levels. Soils of chlorotic stands contained large amounts of extractable inorganic nitrogen and phosphorus. The possible causal relationships between the observed iron deficiency and the differences in elemental composition of needles and soils are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 77
    ISSN: 1573-5036
    Keywords: farming system ; fertilization ; magnesium ; nitrogen ; potassium ; potato ; proteolytic enzymes ; senescence ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of fertilization on senescence and nutrient remobilization in potato leaves was investigated in two farming systems on a soil with a poor potassium availability. The ‘Conventional’ farming system followed good local practices including industrial products, while in the ‘Bio-Dynamic’ farming system industrial fertilizers and synthetic pesticides were avoided. Potassium concentrations in the dry matter of mature leaves varied over a wide range. Nitrogen compounds (protein, chlorophyll) were less affected, and phosphorus concentrations in the dry matter were similar. Magnesium and potassium concentrations in the leaves were negatively correlated. In both farming systems senescence was advanced in plants with a low nutrient supply. Alkaline pyrophosphatase and aminopeptidase activities (in general highest in expanding and mature leaves) were lower and endopeptidase activities peaked earlier on plots with low fertilizer levels. A high percentage of potassium was remobilized from senescing leaves on unfertilized plots, but the phosphorus concentration remained high at the end of the season. The results suggest that the differential net remobilization of nitrogen, phosphorus and potassium depended on actual source/sink relations in the plants.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 78
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 157 (1993), S. 175-184 
    ISSN: 1573-5036
    Keywords: barley ; extraction ; malting quality ; mineralisation ; nitrogen ; 15N-labelling ; potassium chloride ; uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The potentially mineralisable nitrogen extracted from 10 soils by two methods involving boiling with dilute KCl were compared with the actual uptake of soil N by spring barley in the field, as determined with the use of 15N-labelled fertiliser. Generally good correlations were found for those soils which had previously been cropped with cereals (defined in Great Britain as either ‘N-Index zero’, or ‘low nitrogen status’ soils, for fertiliser recommendations), with the results obtained by the more severe of the two methods being somewhat better than by the other method. When organic matter content was also taken into account, correlations were improved. Mineral nitrogen in the soil at sowing was highly correlated with potentially mineralisable nitrogen, and with uptake, but this relationship did not hold for soil samples taken in January, well before the likely sowing date. This suggested that early measurement of soil mineral nitrogen (when decisions on cropping are normally made) was not a practicable method for determinining spring fertiliser applications, and that the measure of potentially mineralisable nitrogen appeared more promising in this regard.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 79
    ISSN: 1573-5036
    Keywords: decomposition ; fine roots ; lignin ; litterbags ; nitrogen ; polyphenols ; substrate quality ; trees ; tropical montane rain forest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Decomposition rates, initial chemical composition, and the relationship between initial chemistry and mass loss of fine roots and foliage were determined for two woody tropical species, Prestoea montana and Dacryodes excelsa, over a gradient of sites in two watersheds in the Luquillo Experimental Forest, Puerto Rico. At all locations, fine roots decayed significantly more slowly than foliage during the initial 6 months. Substrate quality of the initial tissue showed marked differences between roots and foliage when using cell wall chemistry, secondary chemistry and total elemental analysis as indices. Quantity of acid detergent fiber (ADF) (non-digestible cell wall fiber) and lignin content were higher for roots than leaves: D. excelsa roots had 55.3% ADF and 28.7% lignin while leaves had 36.2% ADF and 11.8% lignin; P. montana roots had 68.0% ADF and 26.8% lignin while leaves had 48.5% ADF and 16.1% lignin. Aluminum concentrations were higher in fine roots (843 mg kg−1 in D. excelsa, 1500 mg kg−1 in P. montana) than leaves (244 mg kg−1 in D. excelsa, 422 mg kg−1 in P. montana), while calcium concentrations were higher in foliage (5.5 mg g−1 in D. excelsa, 7.8 mg g−1 in P. montana) than roots (3.4 mg g−1 in D. excelsa, 3.1 mg g−1 in P. montana). Nitrogen did not show any trend with tissue or species type. A linear model between mass remaining after 6 months and initial tissue chemistry could be developed only for calcium (r2=0.64).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 80
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 149 (1993), S. 175-184 
    ISSN: 1573-5036
    Keywords: compaction ; Helianthus annuus L. ; leaf expansion ; nitrogen ; osmotic potential ; photosynthesis ; root growth ; soil strength ; turgor ; water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Leaf expansion and growth response of sunflower (Helianthus annuus, L.) to soil compaction were investigated in relation to compaction effects on water relations, nitrogen nutrition, and photosynthesis. A series of field experiments were conducted with plants grown in 20 cm-diameter cylinders with soil bulk densities ranging from 1.2 to 1.7 g cm−3 at the 0–20 cm depth (equivalent to 0.8 to 2.4 MPa soil strength measured with a soil penetrometer). Relative leaf expansion rate (RLER) decreased linearly with increasing soil strength. Smaller plant size in compacted treatments was due not only to slower expansion rates, but also smaller maximum size of individual leaves. Sensitivity of leaf expansion to soil strength was best illustrated by a reduction in RLER and maximum size of the first leaf to emerge in a treatment with only the lower 10–20 cm of the profile compacted (bulk density of 1.7 g cm−3). Root growth was less affected than shoot growth by compaction and root:shoot ratios of compacted treatments were significantly higher than the control. Soil compaction had no significant effect on pre-dawn or midday leaf water potential, osmotic potential or leaf turgor. Specific leaf weight was usually higher in plants grown on compacted soil, and leaf nitrogen and photosynthesis per unit leaf area were either unaffected by treatment or significantly higher in compacted treatments. The results suggest that early growth reduction of sunflower plants grown on compacted soil was more sink- than source-limited with regard to water, nitrogen, and carbon supply. Further evaluation of this hypothesis will require verification that these whole-leaf measurements provided a sufficiently accurate approximation of treatment effects on the dynamic equilibria of expanding cells.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 81
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 153 (1993), S. 287-293 
    ISSN: 1573-5036
    Keywords: grain protein concentration ; grain yield ; nitrogen ; tritordeum ; triticale ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The new species of cereal × Tritordeum Ascherson et Graebner (Hordeum chilense Roem. et Shultz × Triticum ssp.) has a grain protein concentration (GPC) of up to 25%. The relationship between GPC and yield, and the factors responsible for the high GPC of tritordeum were examined and compared in field experiments. Three experimental tritordeum lines, two early and a later released (recombined and secondary tritordeums) were compared to wheat (cv. Cajeme) and triticale cultivars (cv. Trujillo). GPC's were 19%–22% for recombined tritordeums, 16% for the secondary tritordeum, 12–15% for wheats and 11% for triticale. Grain yields of the recombined and secondary tridordeum were 17–33% and 45–57% that of the wheats and triticale, respectively. Reducing grain sink size by spikelet removal resulted in an increased GPC of remaining grains. Considering all species together there were a strong inverse relationship between GPC and grain yield (GY) per main ear (GPC=26−4.76 ln GY; r2=0.82). In another experiment, frost damage to an early sown treatment of wheat reduced sink size. Harvest index (HI) of early sown wheat was reduced from 0.45 to 0.19, values comparable to that of tritordeum. Having similar HI, the GPC of the early sown wheat was the same as an early sown tritordeum (around 18%). Data for total N uptake and the N concentration of plant tissue during the growing season indicated that enhanced N uptake and remobilisation were not responsible for tritordeum's high GPC. These results suggest that the high GPC of the early lines of tritordeum is a consequence of the small grain yield concentrating the grain protein.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 82
    ISSN: 1573-5036
    Keywords: clover ; grass ; leaching ; lysimeter ; nitrogen ; 15N ; nutrient balances ; nutrient uptake ; pasture ; subsoiling ; sulphur ; 35S
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Synthetic cow urine labelled with 35S and 15N was applied to large, undisturbed, monolith lysimeters sampled from subsoiled and non-subsoiled areas of a grass/clover pasture. For one year following the urine application, the lysimeters were subjected to a combination of natural rainfall, simulated rainfall and simulated flood irrigations. Drainage from the lysimeters was sampled regularly and monthly (approx.) pasture cuts taken. At the end of the year, the lysimeters were destructively sampled in 50 mm depth increments for soil analysis. Leachates, plant samples and soil samples were analysed for 35S and 15N. There were no significant differences in plant uptake of 35S and 15N between the subsoiled and nonsubsoiled lysimeters. Initially grass showed a higher degree of labelling than clover. Total amounts of 35S and 15N leached from the subsoiled lysimeters were approximately twice that leached from the nonsubsoiled ones. Leaching patterns differed substantially between the two nutrients. Total recoveries of 35S (in plants, leachates and soil extracts) accounted for 82% of the applied 35S for the subsoiled lysimeters and 72% for non-subsoiled ones. The unrecovered 35S is considered to have been incorporated into soil organic matter. Total recoveries of 15N (in plants, soil and leachates) were similar to those for 35S, but unrecovered 15N is attributed to loss by denitrification.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 83
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 251 (1993), S. 49-58 
    ISSN: 1573-5117
    Keywords: lake littoral ; detritus ; macrophytes ; decomposition ; nitrogen ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The importance of detritus varies greatly among shore zones of lakes, but in a large majority of these regions detrital pathways prevail. Aside from a great spatial and seasonal variability, macrophytes and bottom sediments appear to be dominant stores of nutrients in these habitats. Macrophytes hold a central position in nutrient cycling in the shore-littoral lake zones. They are the main source of autochthonous detritus as they prevail in the total biomass of littoral organisms, and they are only rarely available as direct food of consumers. Various processes and interactions determine the role of macrophytes in nutrients dynamics. These are: the intensity of nutrient uptake and translocation, release of nutrients by healthy plants and from decomposing plants, exchange of elements between macrophytes and their periphyton, as well as interception of seston by macrophyte stands. Particular plant species differ in their time of dying and susceptibility to decomposition. The changes in decomposing material (size structure of particles and nutrient content) mean that detritus in various stages of decomposition differs in its role in trophic dynamics of shore-littoral lake zones. Several types of shore regions as regards detritus sources and retention level are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 84
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 251 (1993), S. 65-72 
    ISSN: 1573-5117
    Keywords: Frozen littoral ; frost heave ; nutrient release ; nitrogen ; phosphorus ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In winter, the littoral substrate of ecotonal zones along north Swedish rivers and lakes freezes. Even if the littoral is flooded at freeze-up, the ice freezes solid to the shore substrate due to low temperature combined with a gradual decrease in water level during winter. Frost conditions were studied during an 8-year period along eight littoral transects in the River Vindelälven and adjacent riverside lagoons. Heavy frost heave with formation of needle ice was observed in several places. Nutrient release was suggested to take place due to the frost process. Sediment was taken to a laboratory experiment where samples with sediment and water were kept under unfrozen and frozen conditions during six months. N (total-N) and Ca were significantly released to the water, while P (total-P) did not show any clear movement from the frozen sediment. The results indicate that frost processes in ecotonal zones are involved in the movement of nutrients between sediment and water.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 85
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 251 (1993), S. 95-101 
    ISSN: 1573-5117
    Keywords: Land/stream ecotones ; bank runoff ; aerial drift ; retention ; carbon ; nitrogen ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ‘stream’ ecosystem is described as a three-layered system, vertically as well as horizontally. The land/stream ecotone is defined as the area where lotic and edaphic characters overlap at the time of observation. The study site — ‘Oberer Seebach’, near the Biological Station Lunz — and the methods employed are described. Only a surprisingly small fraction (less than 10 %) of total imports is transported across the surface ecotones. The driving variables determining bank runoff and aerial drift are described and discussed. The importance of channel areas which are periodically flooded is stressed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 86
    ISSN: 1573-5095
    Keywords: eucalypt ; regrowth forest ; litter ; litterfall ; fertilizer ; nitrogen ; nutrient cycling ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of fertilizer treatment on nutrient transfers to the forest floor were examined in regrowth Eucalyptus diversicolor F. Muell. forest. Dry weight and nutrient content of leaf litterfall and total litterfall were measured for 3 years in a stand to which two levels of N (0, 200 kg ha-1 year-1) were applied each year at each of three levels of a single initial application of P (0, 30, 200 kg ha-1). Annual accessions of litter to the forest floor were significantly increased by additions of both N (by 17%, 18% and 21% in the 3 years) and 200 kg P ha-1 (by 8%, 8% and 4% in the 3 years) but there was no interaction between effects of N and P treatments. Fertilizer application also had a significant effect on the nutrient content of leaf litterfall and total litterfall. Concentration of N in leaf litterfall was 9% to 23% greater on plots treated with N fertilizer compared to untreated plots. The amounts of N in litterfall were about 30% greater on N-treated compared to untreated plots. On plots treated with 200 kg P ha-1, P concentrations in leaf litter were 50% to 100% greater than in litter from plots receiving no P. Application of 200 kg P ha-1 increased the amounts of P in annual litterfall by 32% to 87%. The greatest increase in P accessions occurred soon after fertilizer treatment. The amounts of Ca, K, and Na in litterfall were also significantly increased by fertilizer application. For Ca and K this was due partly to increases in element concentrations in litterfall following application of treatments. The effect of fertilizers on internal recycling of plant nutrients and on litter accumulation and nutrient dynamics in forest floor litter is discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 87
    ISSN: 1573-5117
    Keywords: retention ; core-sampling ; phosphorus ; nitrogen ; resuspension ; export
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a Danish lowland river system intensive measurements were made, in four 80 m reaches, of the nitrogen (N) and phosphorus (P) stored in the stream sediment. The results were used for calculation of the total retention in the river system during two summers (June to August). In addition, the mobilization of nutrients from the stream bottom in autumn 1987 was compared with the export from the watershed. During the study period (June 1987 to September 1988) the amounts of N and P stored in stream reaches were determined fortnightly using a core-sample technique. In reaches dominated by submersed macrophytes, 25–40 g N m−2 and 20–30 g P m−2 were stored during two summers, against only 10–15g N and P m−2 for sandy and gravely reaches. In riparian zones with emergent macrophytes the retention was even higher than in the submersed macrophytes. Gross retention exceeded net retention by a factor of two to three. Net retention of P in the river system during the summer of 1987 was equal to the summer export from the watershed. On an annual basis, retention in the summer constituted 20% of the P export. In contrast, retention in the summer of 1988 amounted to 60% of the total P export during the same period (38% reduction) and 22% in comparison with the annual export. The corresponding figures for N were lower, showing reductions of 16% and 12% of the export of total N in the two summer periods, and about 1% of the annual exports. In September 1987 6.4% of the total N export and 65% of the total P export from the watershed consisted of resuspended material. In 1987 the N and P retained during the summer was almost completely resuspended during storm events during September to November.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 88
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 271 (1993), S. 97-108 
    ISSN: 1573-5117
    Keywords: Myriophyllum aquaticum ; parrotfeather ; nitrogen ; phosphorus ; mass flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The contribution of sediment interstitial water and the water column to the transpiration stream of Myriophyllum aquaticum (Vellozo) Verdcourt was determined to estimate the significance of mass flow in supply of sediment nutrients for plant growth. Sediment interstitial water accounted for about 2% of the water transpired over a 37 day period. Because of the small volume of water that originated in the sediment we concluded that mass flow did not significantly enhance nutrient supply to the roots of M. aquaticum. Relative growth rate (RGR) of adventitious, water roots was greater than whole plant RGR, and RGR of sediment roots was not significantly different from zero, indicating a shift in the biomass allocation after emergence of the apical meristem into the air. Water use, measured by the transpiration coefficient, averaged 260 ml H2O mg DW-1, which is similar to C-4 terrestrial plants. M. aquaticum has leaf characteristics commonly associated with xerophytic habitats. These characteristics may be necessary if a high transpiration rate and a mechanical requirement for high cell turgor pressure, required by a reliance upon hydrostatic pressure for support of the aerial stems, are mutually exclusive because of morphological constraints on hydraulic conductivity.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 89
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 251 (1993), S. 143-148 
    ISSN: 1573-5117
    Keywords: Danube Delta ; flood zones ; phosphorus ; nitrogen ; eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The change of concentration of total reactive phosphorus (TRP) and dissolved inorganic nitrogen (DIN) was studied in the lower Danube river and in selected lakes situated in the wetland area of the Danube Delta. The differences Danube Delta in nutrient concentration in the river waters entering the delta and the delta in different sites (especially lakes) of the wetland area are considered to reflect retention in the system. The highest retention was found in periods of moderate and low water level when the surface-to-volume ratio of the lakes was high. In these periods the in-lake concentration of TRP and DIN could be as low as 11 and 23% of the values found in the inflowing river.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 90
    Electronic Resource
    Electronic Resource
    Springer
    Biogeochemistry 22 (1993), S. 157-178 
    ISSN: 1573-515X
    Keywords: Serengeti ; productivity ; precipitation ; nitrogen ; grazing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract In the Serengeti National Park, Tanzania, precipitation and soil nitrogen vary greatly between northwestern tallgrass areas and southeastern shortgrass areas, with the tallgrass having higher total precipitation and lower soil fertility. We used a model of grassland productivity, carbon/nitrogen cycling, and abiotic factors to test the hypothesis that tallgrass productivity is limited primarily by nitrogen availability while shortgrass productivity is limited by water. Under observed grazing intensities and ungrazed conditions, precipitation exerted primary control over grassland productivity for both regions, with differences in soil texture mediating soil water availability to the grasses. Mineral nitrogen availability interacted with water availability to influence productivity at precipitation levels ⩾ 130% of the mean. Nitrogen mineralization and precipitation were positively related for each grassland type, however, nitrification varied both between grassland types and between grazed and ungrazed conditions. Combined mineralization and nitrification could not maintain soil mineral nitrogen levels in the face of plant nitrogen uptake stimulated by increased precipitation, thus providing the mechanism by which nitrogen becomes a secondary limiting factor for both grasslands. Model experiments indicated that the pattern of primary limitation by precipitation and secondary limitation by nitrogen was robust to model assumptions concerning ungulate deposition of urine and dung nitrogen to the soil.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 91
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 36 (1993), S. 79-90 
    ISSN: 1573-0867
    Keywords: agriculture ; Sahel ; Sudan ; Mali ; cotton ; fertilization ; nutrient ; soil ; soil degradation ; depletion ; nutrient ; nutrient balance ; nitrogen ; phosphorus ; potassium ; calcium ; magnesium ; acidification ; sustainability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The degree of soil mining by agricultural production in Southern Mali is assessed by calculating nutrient balances: differences between the amount of plant nutrients exported from the cultivated fields, and those added to the fields. Export processes include extraction by crops, losses due to leaching, to erosion, and to volatilization and denitrification. Inputs include applications of fertilizer and manure, restitution of crop residues, nitrogen fixation, atmospheric deposition of nutrients in rain and dust, and enrichment by weathering of soil minerals. Nutrient balances are calculated for N, P, K, Ca, and Mg. Both pessimistic and optimistic estimates are given. The resulting figures indicate, even when the most optimistic estimates are used, large deficits for nitrogen, potassium and magnesium. For the region as a whole, the calculated deficits are -25 kg N/ha,-20 kg K/ha, and -5 kg Mg/ha. Further, acidification is to be expected, in particular in areas where cotton is grown. The deficits are caused by traditional cereal crops, but also by cotton and especially by groundnut. The latter two crops are fertilized, but insufficiently. It is important to note, that the negative figures are not automatic recommendations for application of a specific amount of additional fertilizer. For phosphorus and calcium the balance of the region as a whole appears to be about in equilibrium, but locally large variations may occur. Erosion and denitrification are important causes of nutrient loss, accounting respectively for 17 and 22% of total nitrogen exports. Atmospheric deposition and weathering of minerals in the soil are still important nutrient inputs that contribute as much as nutrients as organic and mineral fertilizer combined. Nutrient depletion is very large in comparison to the amount of fertilizer applied. Drastic options, such as doubling the application of fertilizer or manure, or halving erosion losses, even if feasible, would still not be enough to make up for the calculated deficits. The annual value of withdrawn nutrients, if related to prices of fertilizers, varies between 10,000 and 15,000 FCFA/ha (40-60 US $/ha). Since the estimated average gross margin from farming in this area is 34,000 FCFA/ha (123 US $/ha), soil mining appears to provide an amount equal to 40% of farmers' total income from agricultural activities.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 92
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 34 (1993), S. 59-65 
    ISSN: 1573-0867
    Keywords: Barley ; environment ; nitrogen ; seeding rate ; yield ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was conducted to determine the effects of rainfall and temperature during the growing season, seed rate, and N rate on grain yield and yield components of winter barley (Hordeum vulgare L.) on a Typic Haploxeralf soil in Santa Olalla (Toledo), Spain in 1986/7, 1987/8, and 1988/9. Two experiments were conducted each year using the 6-rowed variety ‘Barbarrosa’ and the 2-rowed variety ‘Reinette’. Both experiments used seed rates of 80, 160, and 240 kg ha−1 as whole plot treatments, and N rates of 0, 40, 80, 120, and 160 kg ha−1 as subplots. Responses to N depended on both the quantity and distribution of rainfall during the growing season, and temperature during grain fill. In the high rainfall, moderate grain-fill temperature year, increasing the N rate from 0 to 160 kg ha−1 increased grain yields (by 2.3 t ha−1), straw yields and the harvest index while maintaining the kernel weight for both varieties. In the low rainfall, high grain-fill temperature year, N rate had little influence on grain yield, but increased the straw yield, which reduced the harvest index, and also decreased kernel weight. Seed rate had no influence on grain yields even though wide variation in N rates, rainfall and temperatures occurred in the three-year study. Results from this study indicated that strategies to reduce (or avoid) water/high temperature stress during grain fill are necessary to assure more uniform yield responses to N application across years. However, decisions about seed rate can be made independently for conditions similar to those in this study.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 93
    Electronic Resource
    Electronic Resource
    Springer
    Agroforestry systems 21 (1993), S. 1-10 
    ISSN: 1572-9680
    Keywords: silvo-pasture ; compaction ; cattle traffic ; penetrometer ; bulk density ; nitrogen ; seedlings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper reports on the extent of soil compaction due to cattle traffic around hardwood and softwood tree seedlings established in existing pasture, and subject to cattle pressure. A higher soil penetrometer resistance in the grazed areas pointed to a significant change in soil structure (i.e. dry bulk density) as a result of cattle traffic in the area. In a related experiment comparing the effect of three different levels of soil compaction on tree seedling growth and nitrogen cycling it was found that water infiltration and nitrogen uptake were reduced in soils treated with a medium and high level of compactive effort. This resulted in a slower rate of growth of the tree seedlings. The addition of an additional nitrogen source improved seedling growth in both the medium and high density compactive treatments.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 94
    ISSN: 1572-9699
    Keywords: survival ; starvation ; stress ; Vibrio ; carbon ; nitrogen ; phosphorus ; protein synthesis ; carbon starvation stimulon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Non-differentiating bacteria adapt to starvation induced growth arrest by a complex turn-on/turn-off pattern of protein synthesis. This response shows distinct similarities with those of spore formation in differentiating organisms. A substantial amount of information on the non-growth biology of non-differentiating bacteria can be derived from studies onVibrio strains. One important result is that carbon rather than nitrogen or phosphorus starvation leads to the development of a starvation and stress resistant cell in these organisms. Hence, we have attempted to characterize the carbon starvation stimulon. By the use of two-dimensional gel electrophoresis of pulse-labelled cells and transposon mutagenesis, using reporter gene constructs, the identity and function of some members of the carbon starvation stimulon have been elucidated. Moreover, regulatory genes of the starvation response have been identified with these techniques. Current studies primarily address the identity and function of these genes. The role of transcript modification and stability for both long term persistence during starvation as well as the efficient recovery of cells which occurs upon nutrient addition is also addressed. It is suggested that an understanding of the functionality of the translational machinery is essential for the understanding of these adaptive pathways. This contribution also discusses the diversity of the differentiation-like response to starvation in different bacteria and whether a general starvation induced programme exists.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 95
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 20 (1993), S. 195-212 
    ISSN: 1572-9729
    Keywords: denitrification ; lake ; mass balance ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen mass balances for seven unproductive lakes and 20 forested catchments in central Ontario were measured between 1977 and 1989. Average annual lake denitrification rates calculated with the N/P ratio method were strongly correlated with summer anoxic factor (extent of surficial sediment anoxia) whereas denitrification rates calculated with a210Pb sediment N accumulation method were poorly correlated with the anoxic factor suggesting that the N/P method is superior. Substantial denitrification occurred in all lakes — an average of 36% of TN inputs or 75% of the net gain. On a regional area-weighted basis, 67% of bulk atmospheric TN deposition was stored or denitrified terrestrially, 12% was denitrified in lakes, 4% was stored in lake sediments, and 17% was exported from lakes. N/P ratios were generally less in streams than in precipitation suggesting preferential N retention in catchments, whereas the N/P ratios in lake outputs were slightly higher than lake input ratios, suggesting preferential P retention in lakes. This is consistent with the notion that P-limited lakes can exist adjacent to N-limited forests.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 96
    Electronic Resource
    Electronic Resource
    Springer
    Plasma chemistry and plasma processing 13 (1993), S. 613-632 
    ISSN: 1572-8986
    Keywords: Aluminum nitride ; aluminum ; nitrogen ; hydrogen ; ammonia ; thermal plasma ; transferred plasma ; emission spectroscopy ; sintering ; ultrafine powder ; nanophase ; composite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract Ultrafine particles of aluminum tnitride (AIN) arc produced by a transferred an plasma. Two devices are used: a transferred arc plasma on aluminum natal in nitrogen or nitrogenlammonia atmospheres, and a item concept of transferred arc plasma when, DC anode and cathode ares are coupled together above an alumintun melt. Equilibrium chemical compositions mere calculated. The temperature distributions in the plasma are measured hr emission spectroscopy Flit, powder, made from 99.8%, aluminum ingot, it as analyzed and confirmed to be 99.3%, of hexagonal phase aluminum nitride. In othertests, from 99.99% aluminum ingot, a translucent AIN vinter was obtained. The densification behavior was assessed by hot pressing and by pressureless sintering, with and without additives. The thermal conductivities are given.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 97
    Electronic Resource
    Electronic Resource
    Springer
    International journal of thermophysics 14 (1993), S. 699-725 
    ISSN: 1572-9567
    Keywords: argon ; caloric properties ; density ; equation of state ; Helmholtz function ; methane ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In order to describe the thermodynamic behavior of methane, argon, and nitrogen in the so-called “natural-gas region,” namely, from 270 to 350 K at pressures up to 30 MPa as accurate as possible with equations of a very simple form, new equations of state for these three substances have been developed. These equations are in the form of a fundamental equation in the dimensionless Helmholtz energy; for calculating the pressure or the density, the corresponding equations explicit in pressure are also given. The residual parts of the Helmholtz function representing the behavior of the real gas contain 12 fitted coefficients for methane, 8 for argon, and 7 for nitrogen. The thermodynamic relations between the Helmholtz energy and the most important thermodynamic properties and the needed derivatives of the equations are explicitly given; to assist the user there is also a table with values for computer-program verification. The uncertainties when calculating the density ρ, the speed of sound w, the isobaric specific heat capacity c p, and the isochoric specific heat capacity c v are estimated as follows. For all three substances it is Δρ/ρ≤±0.02 % for p≤ 12 MPa and Δρ/ρ ≤ ±0.05% for higher pressures. For methane it is Δw/w≤±0.02% for p≤10 MPa and Δw/w≤+-0.1% for higher pressures; for argon it is Δw/w≲-0.1 % for p≤ 7 MPa, Δw/w≤±0.3 % for 7 〈p≤30 MPa; and for nitrogen it is Δw/w≤±0.1% for p≤1.5 MPa and Δw/w±0.5% for higher pressures. For all three substances it is Δc p/c p≤±1 % and ΔC v/C v≤±1 % in the entire range.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 98
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 251 (1993), S. 199-209 
    ISSN: 1573-5117
    Keywords: spring ; karst ; interstitial aquifer ; nitrogen ; carbon ; organic matter retention
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The flow of groundwater through the sediment layer (underflow or hyporheic zone) of streams and at the origin of streams can influence organic matter uptake dynamics of floodplain. The River Rhône floodplain has limestone foothills. Here we studied 2 karstic and 2 interstitial springs differing by aquifer geology. Organic matter, physico-chemical conditions were compared between these springs during two seasons (from March to September 1989) and at different depths (0, −20 cm, −40 cm). Temperatures indicated large differences in underflow between springs, in their relation to the surrounding environment, and between seasons. Springs are well oxygenated, with differences between layers. Cultivated fields supply interstitial springs with nitrates, and pools are nutrient traps. DOC was heterogeneous in space and time and correlates with VFPOC. Particulate nutrients were correlated with available surface area of sediment grains. Physical conditions of each spring were prominent in determining storage and turnover of organic matter. Each spring, by its own characteristics and dynamics regulating stability and turnover, had an effect or control on storage, transport and retention of organic matter (quality, quantity). These springs offer an example of the heterogeneity, and give a view of the diversity of patches within a floodplain. The data suggest that groundwater flow of springs may be a major factor in the functioning of floodplain tributaries.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 99
    ISSN: 1573-5117
    Keywords: land drainage ; nitrogen ; phosphorus ; areal coefficients ; agricultural streams ; riparian ecotones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During the 19th and the first half of the 20th century, approximately 300 km2 of lakes and wetlands, representing 29% of the River Kavlingean catchment in Southern Sweden, were drained to make land available for agriculture. Published accounts of nutrient loads from the catchment indicated that until the mid 20th century, factories and urban point sources were the major contributors of both nitrogen and phosphorus. By the middle of the 20th century, the construction of sewage treatment plants had effectively reduced phosphorus pollution. Concurrently, the land drained in the previous century underwent a more intense cultivation, with productivity being maintained by commercial fertilizers. Subsequently, net nutrient loads from agriculture continued to increase, reaching an annual load of 2652 tons total-nitrogen and 70 tons total-phosphorus for the River Kävlingeån. Whilst high nutrient leakage from agricultural watersheds may be a problem which is only recently recognized, it had its origins in nearly a hundred years of commonly accepted agricultural policy. To assess the importance of agriculture as the major source of nutrients to the River Kävlingeån system, three tributary catchment areas, differing in terms of their land use patterns (high, medium and low intensity of agricultural use), were studied and compared with literature figures. Results indicated that agricultural nutrient loss areal coefficients were substantially higher than the literature figures, demonstrating the role of agriculture as source of nutrients to the River Kävlingeån system. The agricultural land use policies of the last fifty years were revealed to be most important with regard to this role. Of such land use policies, the cultivation of the last 10–15% of land employed for agricultural use (primarily riparian ecotones) may be of most significance. The literature indicates that intense agricultural use of this final 10–15% may account for a ca. 50% increase in nitrogen loss. This suggests that one solution to the problem of agricultural diffuse pollution may lie in the restoration and sustainable management of riparian ecotones of agricultural streams.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 100
    ISSN: 1573-5117
    Keywords: littoral ; reed-belt decline ; Phragmites australis ; nitrogen ; eutrophication ; productivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot biomass and nitrogen, accumulated within above-ground plant biomass in autumn, correlate with nitrogen availability indicated by nitrogen content (% DM) of several shoot parts during the height of the growth period. A higher nitrogen percentage of the shoots is correlated with a higher shoot loss and subsequent substitution by branching and tillering during and continuing toward the end of the growth period. A delayed switch from the vegetative to the generative phase reduces the translocation of reserve material to the rhizome. Increasing nitrogen load is at least one of the factors causing instability and reed-belt decline.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...