ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Mixed-bed columns
;
Mixed-mode chromatography
;
Drug metabolites
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The feasibility of using columns prepared by mixing together ion exchange and reversed-phase packing materials, for the simultaneous separation of ionised and unionised molecules, has been examined using a range of test solutes. Columns prepared in this way showed chromatographic properties which were intermediate between those of the individual phases. The dual nature of the retention mechanism allowed the retention of ionisable molecules to be adjusted using pH whilst that of uncharged compounds was unaffected. The simultaneous chromatography of model compounds and their glucuronide, sulphate and glycine conjugates, under a variety of conditions, was demonstrated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02272190
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