ALBERT

Description: Materials, Vol. 11, Pages 1293: The 3 R’s for Platelet-Rich Fibrin: A “Super” Tri-Dimensional Biomaterial for Contemporary Naturally-Guided Oro-Maxillo-Facial Soft and Hard Tissue Repair, Reconstruction and Regeneration Materials doi: 10.3390/ma11081293 Authors: Consuelo C. Zumarán Marcelo V. Parra Sergio A. Olate Eduardo G. Fernández Francisco T. Muñoz Ziyad S. Haidar Platelet-Rich fibrin (PRF) is a three-dimensional (3-D) autogenous biomaterial obtained via simple and rapid centrifugation from the patient’s whole blood samples, without including anti-coagulants, bovine thrombin, additives, or any gelifying agents. At the moment, it is safe to say that in oral and maxillofacial surgery, PRFs (particularly, the pure platelet-rich fibrin or P-PRF and leukocyte and platelet-rich fibrin or L-PRF sub-families) are receiving the most attention, essentially because of their simplicity, cost-effectiveness, and user-friendliness/malleability; they are a fairly new “revolutionary” step in second-generation therapies based on platelet concentration, indeed. Yet, the clinical effectiveness of such surgical adjuvants or regenerative platelet concentrate-based preparations continues to be highly debatable, primarily as a result of preparation protocol variability, limited evidence-based clinical literature, and/or poor understanding of bio-components and clinico-mechanical properties. To provide a practical update on the application of PRFs during oral surgery procedures, this critical review focuses on evidence obtained from human randomized and controlled clinical trials only. The aim is to serve the reader with current information on the clinical potential, limitations, challenges, and prospects of PRFs. Accordingly, reports often associate autologous PRFs with early bone formation and maturation; accelerated soft-tissue healing; and reduced post-surgical edema, pain, and discomfort. An advanced and original tool in regenerative dentistry, PRFs present a strong alternative and presumably cost-effective biomaterial for oro-maxillo-facial tissue (soft and hard) repair and regeneration. Yet, preparation protocols continue to be a source of confusion, thereby requiring revision and standardization. Moreover, to increase the validity, comprehension, and therapeutic potential of the reported findings or observations, a decent analysis of the mechanico-rheological properties, bio-components, and their bioactive function is eagerly needed and awaited; afterwards, the field can progress toward a brand-new era of “super” oro-dental biomaterials and bioscaffolds for use in oral and maxillofacial tissue repair and regeneration, and beyond.

Description: by Edvin Fuglebakk, Nathalie Reuter With remarkable spatial and temporal specificities, peripheral membrane proteins bind to biological membranes. They do this without compromising solubility of the protein, and their binding sites are not easily distinguished. Prototypical peripheral membrane binding sites display a combination of patches of basic and hydrophobic amino acids that are also frequently present on other protein surfaces. The purpose of this contribution is to identify simple but essential components for membrane binding, through structural criteria that distinguish exposed hydrophobes at membrane binding sites from those that are frequently found on any protein surface. We formulate the concepts of protruding hydrophobes and co-insertability and have analysed more than 300 families of proteins that are classified as peripheral membrane binders. We find that this structural motif strongly discriminates the surfaces of membrane-binding and non-binding proteins. Our model constitutes a novel formulation of a structural pattern for membrane recognition and emphasizes the importance of subtle structural properties of hydrophobic membrane binding sites.

Description: by Hugo Cruces-Solís, Zhizi Jing, Olga Babaev, Jonathan Rubin, Burak Gür, Dilja Krueger-Burg, Nicola Strenzke, Livia de Hoz Detecting regular patterns in the environment, a process known as statistical learning, is essential for survival. Neuronal adaptation is a key mechanism in the detection of patterns that are continuously repeated across short (seconds to minutes) temporal windows. Here, we found in mice that a subcortical structure in the auditory midbrain was sensitive to patterns that were repeated discontinuously, in a temporally sparse manner, across windows of minutes to hours. Using a combination of behavioral, electrophysiological, and molecular approaches, we found changes in neuronal response gain that varied in mechanism with the degree of sound predictability and resulted in changes in frequency coding. Analysis of population activity (structural tuning) revealed an increase in frequency classification accuracy in the context of increased overlap in responses across frequencies. The increase in accuracy and overlap was paralleled at the behavioral level in an increase in generalization in the absence of diminished discrimination. Gain modulation was accompanied by changes in gene and protein expression, indicative of long-term plasticity. Physiological changes were largely independent of corticofugal feedback, and no changes were seen in upstream cochlear nucleus responses, suggesting a key role of the auditory midbrain in sensory gating. Subsequent behavior demonstrated learning of predictable and random patterns and their importance in auditory conditioning. Using longer timescales than previously explored, the combined data show that the auditory midbrain codes statistical learning of temporally sparse patterns, a process that is critical for the detection of relevant stimuli in the constant soundscape that the animal navigates through.

Description: by Greg Holmes, Lening Zhang, Joshua Rivera, Ryan Murphy, Claudia Assouline, Lorraine Sullivan, Todd Oppeneer, Ethylin Wang Jabs Activating mutations of fibroblast growth factor receptors (FGFRs) are a major cause of skeletal dysplasias, and thus they are potential targets for pharmaceutical intervention. BMN 111, a C-type natriuretic peptide analog, inhibits FGFR signaling at the level of the RAF1 kinase through natriuretic peptide receptor 2 (NPR2) and has been shown to lengthen the long bones and improve skull morphology in the Fgfr3 Y367C/+ thanatophoric dysplasia mouse model. Here we report the effects of BMN 111 in treating craniosynostosis and aberrant skull morphology in the Fgfr2c C342Y/+ Crouzon syndrome mouse model. We first demonstrated that NPR2 is expressed in the murine coronal suture and spheno-occipital synchondrosis in the newborn period. We then gave Fgfr2c C342Y/+ and Fgfr2c +/+ (WT) mice once-daily injections of either vehicle or reported therapeutic levels of BMN 111 between post-natal days 3 and 31. Changes in skeletal morphology, including suture patency, skull dimensions, and long bone length, were assessed by micro-computed tomography. Although BMN 111 treatment significantly increased long bone growth in both WT and mutant mice, skull dimensions and suture patency generally were not significantly affected. A small but significant increase in the relative length of the anterior cranial base was observed. Our results indicate that the differential effects of BMN 111 in treating various skeletal dysplasias may depend on the process of bone formation targeted (endochondral or intramembranous), the specific FGFR mutated, and/or the specific signaling pathway changes due to a given mutation.

Description: by Irineu Loturco, Bret Contreras, Ronaldo Kobal, Victor Fernandes, Neilton Moura, Felipe Siqueira, Ciro Winckler, Timothy Suchomel, Lucas Adriano Pereira The capacity to rapidly generate and apply a great amount of force seems to play a key role in sprint running. However, it has recently been shown that, for sprinters, the technical ability to effectively orient the force onto the ground is more important than its total amount. The force-vector theory has been proposed to guide coaches in selecting the most adequate exercises to comprehensively develop the neuromechanical qualities related to the distinct phases of sprinting. This study aimed to compare the relationships between vertically-directed (loaded and unloaded vertical jumps, and half-squat) and horizontally-directed (hip-thrust) exercises and the sprint performance of top-level track and field athletes. Sixteen sprinters and jumpers (including three Olympic athletes) executed vertical jumps, loaded jump squats and hip-thrusts, and sprinting speed tests at 10-, 20-, 40-, 60-, 100-, and 150-m. Results indicated that the hip-thrust is more associated with the maximum acceleration phase (i.e., from zero to 10-m; r = 0.93), whereas the loaded and unloaded vertical jumps seem to be more related to top-speed phases (i.e., distances superior to 40-m; r varying from 0.88 to 0.96). These findings reinforce the mechanical concepts supporting the force-vector theory, and provide coaches and sport scientists with valuable information about the potential use and benefits of using vertically- or horizontally-based training exercises.

Description: by Pei-Chun Kao, Michaela A. Pierro, Konstantina Booras Cognitive-motor interference, a negative influence on the performance of one or both tasks, is manifested when simultaneously performing a cognitive and a motor task. Motor fatigue reduces the ability of generating a required force level. However, little is known about the effects of motor fatigue on the cognitive-motor dual-tasking performance, an important capability during our daily lives. This study investigated how motor fatigue affects dual-task walking performance. Eighteen healthy younger adults walked on a treadmill under three different conditions: walking only, walking while receiving the Paced Auditory Serial Addition Test (PASAT) or a modified Stroop test before and after a lower-extremity fatiguing exercise. We computed dynamic margins of stability (MOS), step and joint kinematic variability, and short-term local divergence exponent (LDE) of the trunk motion. We found that subjects had similar values of short-term LDE during all conditions, indicating that local stability was not affected by the motor fatigue or dual-task conditions. Compared to the baseline, subjects had significantly greater mean MOS after the fatiguing exercise by walking with greater step length and width while having significantly greater gait variability. In contrast, subjects walked with similar mean MOS but significantly less gait variability during the dual-task conditions, indicating that subjects used different adaptive strategies when walking with motor fatigue and during dual-task conditions. There were no significant differences in the number of errors for the two cognitive tests before and after the fatiguing exercise. The current findings demonstrate that motor fatigue does not affect cognitive but motor performance in younger adults.

Description: by Insu Lee, Daegyu Kim, Ga-Lahm Park, Tae-Joon Jeon, Sun Min Kim When living tissues are injured, they undergo a sequential process of homeostasis, inflammation, proliferation and maturation, which is called wound healing. The working mechanism of wound healing has not been wholly understood due to its complex environments with various mechanical and chemical factors. In this study, we propose a novel in vitro wound healing model using a microfluidic system that can manipulate the topography of the wound bed. The topography of the extracellular matrix (ECM) in the wound bed is one of the most important mechanical properties for rapid and effective wound healing. We focused our work on the topographical factor which is one of crucial mechanical cues in wound healing process by using various nano-patterns on the cell attachment surface. First, we analyzed the cell morphology and dynamic cellular behaviors of NIH-3T3 fibroblasts on the nano-patterned surface. Their morphology and dynamic behaviors were investigated for relevance with regard to the recovery function. Second, we developed a highly reproducible and inexpensive research platform for wound formation and the wound healing process by combining the nano-patterned surface and a microfluidic channel. The effect of topography on wound recovery performance was analyzed. This in vitro wound healing research platform will provide well-controlled topographic cue of wound bed and contribute to the study on the fundamental mechanism of wound healing.

Description: by Tilen Koklic, Iztok Urbančič, Irena Zdovc, Majda Golob, Polona Umek, Zoran Arsov, Goran Dražić, Štefan Pintarič, Martin Dobeic, Janez Štrancar Bacterial infections acquired in healthcare facilities including hospitals, the so called healthcare acquired or nosocomial infections, are still of great concern worldwide and represent a significant economical burden. One of the major causes of morbidity is infection with Methicillin Resistant Staphylococcus aureus (MRSA), which has been reported to survive on surfaces for several months. Bactericidal activity of copper-TiO 2 thin films, which release copper ions and are deposited on glass surfaces and heated to high temperatures, is well known even when illuminated with very weak UVA light of about 10 μW/cm 2 . Lately, there is an increased intrerest for one-dimensional TiO 2 nanomaterials, due to their unique properties, low cost, and high thermal and photochemical stability. Here we show that copper doped TiO 2 nanotubes produce about five times more ·OH radicals as compared to undoped TiO 2 nanotubes and that effective surface disinfection, determined by a modified ISO 22196:2011 test, can be achieved even at low intensity UVA light of 30 μW/cm 2 . The nanotubes can be deposited on a preformed surface at room temperature, resulting in a stable deposition resistant to multiple washings. Up to 10 3 microorganisms per cm 2 can be inactivated in 24 hours, including resistant strains such as Methicillin-resistant Staphylococcus aureus (MRSA) and Extended-spectrum beta-lactamase Escherichia coli ( E . coli ESBL). This disinfection method could provide a valuable alternative to the current surface disinfection methods.

Description: by Tim J. Sloan, Jonna Jalanka, Giles A. D. Major, Shanthi Krishnasamy, Sue Pritchard, Salah Abdelrazig, Katri Korpela, Gulzar Singh, Claire Mulvenna, Caroline L. Hoad, Luca Marciani, David A. Barrett, Miranda C. E. Lomer, Willem M. de Vos, Penny A. Gowland, Robin C. Spiller Background & aims Ingestion of poorly digested, fermentable carbohydrates (fermentable oligo-, di-, mono-saccharides and polyols; FODMAPs) have been implicated in exacerbating intestinal symptoms and the reduction of intake with symptom alleviation. Restricting FODMAP intake is believed to relieve colonic distension by reducing colonic fermentation but this has not been previously directly assessed. We performed a randomised controlled trial comparing the effect of a low FODMAP diet combined with either maltodextrin or oligofructose on colonic contents, metabolites and microbiota. Methods A parallel randomised controlled trial in healthy adults (n = 37). All subjects followed a low FODMAP diet for a week and supplemented their diet with either maltodextrin (MD) or oligofructose (OF) 7g twice daily. Fasted assessments performed pre- and post-diet included MRI to assess colonic volume, breath testing for hydrogen and methane, and stool collection for microbiota analysis. Results The low FODMAP diet was associated with a reduction in Bifidobacterium and breath hydrogen, which was reversed by oligofructose supplementation. The difference in breath hydrogen between groups post-intervention was 27ppm (95% CI 7 to 50, P

Description: by Geethika Reddi, Kali Pruss, Kathryn L. Cottingham, Ronald K. Taylor, Salvador Almagro-Moreno Vibrio cholerae O1, the etiological agent of cholera, is a natural inhabitant of aquatic ecosystems. Motility is a critical element for the colonization of both the human host and its environmental reservoirs. In this study, we investigated the molecular mechanisms underlying the chemotactic response of V . cholerae in the presence of some of its environmental reservoirs. We found that, from the several oligosaccharides found in mucin, two specifically triggered motility of V . cholerae O1: N -acetylneuraminic acid (Neu5Ac) and N -acetylglucosamine (GlcNAc). We determined that the compounds need to be internally catabolized in order to trigger motility of V . cholerae . Interestingly, the catabolism of Neu5Ac and GlcNAc converges and the production of one molecule common to both pathways, glucosamine-6-phosphate (GlcN-6P), is essential to induce motility in the presence of both compounds. Mutants unable to produce GlcN-6P show greatly reduced motility towards mucin. Furthermore, we determined that the production of GlcN-6P is necessary to induce motility of V . cholerae in the presence of some of its environmental reservoirs such as crustaceans or cyanobacteria, revealing a molecular link between the two distinct modes of the complex life cycle of V . cholerae . Finally, cross-species comparisons revealed varied chemotactic responses towards mucin, GlcNAc, and Neu5Ac for environmental (non-pathogenic) strains of V . cholerae , clinical and environmental isolates of the human pathogens Vibrio vulnificus and Vibrio parahaemolyticus , and fish and squid isolates of the symbiotic bacterium Vibrio fischeri . The data presented here suggest nuance in convergent strategies across species of the same bacterial family for motility towards suitable substrates for colonization.

Description: by Anne C. Wheeler, Camila V. Ventura, Ty Ridenour, Danielle Toth, Lucélia Lima Nobrega, Lana Claudia Silva de Souza Dantas, Camilla Rocha, Donald B. Bailey Jr., Liana O. Ventura The recent Zika outbreak and its link to microcephaly and other birth defects in infants exposed in utero have garnered widespread international attention. Based on the severity of birth defects the extent of impairment in these infants is expected to be profound; however, virtually nothing is known regarding the developmental and behavioral sequela of congenital Zika syndrome. This pilot study collected parent-reported patterns of development and sleep in 47 infants with confirmed congenital Zika syndrome who are being followed for clinical services at the Altino Ventura Foundation (FAV) in Recife, Brazil. With assistance from clinicians at FAV, caregivers completed Brazilian Portuguese versions of the Ages and Stages Questionnaire, 3 rd edition (ASQ-3) and the Brief Infant Sleep Questionnaire (BISQ). All infants were between 13–22 months of age at the time of the assessment. At 16 months of age, none of the children displayed age appropriate developmental skills. Most (~ 75%) mastered some communication and gross motor skills at around a 6–8-month level. Communication and gross motor skills were relative strengths for the sample, while problem-solving and fine motor skills were relative weaknesses. Sleep was noted to be a problem for around 18% of the sample. In utero exposure to the Zika virus will have lifelong consequences for affected children and their families. Understanding the developmental and behavioral trajectories of affected infants will help identify appropriate family supports to improve quality of life.

Description: by Veronique Deschodt-Arsac, Romain Lalanne, Beatrice Spiluttini, Claire Bertin, Laurent M. Arsac Introduction Heart rate variability biofeedback (HRV-BFB) training, a method whereby one controls an unusually low breathing rate to reach cardiac coherence, has been shown to reduce anxiety and improve cardiac autonomic markers in diseased people, but much less is known about HRV-BFB benefits in healthy people. Here we investigated potential benefits in young competitors experiencing stress during university examinations as well as persistence of benefits after HRV-BFB training cessation. Methods A group of sports students (n = 12) practiced 5-min HRV-BFB training twice a day for 5-weeks using URGOfeel ® (URGOTECH) and was compared to a control group (n = 6). University examinations occurred immediately after HRV-BFB training (Exam1), then 12-weeks later (Exam2). Anxiety markers and cardiac autonomic markers were assessed at baseline, Exam1 and Exam2. Principal Component Analyses (PCA) that combined all these markers were computed at Exam1 and Exam2 to emphasize covariations. Results At Exam 1, immediately after HRV-BFB training cessation, the experimental group demonstrated greater autonomic markers but similar states of anxiety when compared to the Control group. Twelve weeks later at Exam2, autonomic markers were greater and anxiety scores were lesser among the experimental group. PCA highlighted covariations only within cardiac autonomic markers at Exam1. Rather, variations in cardiac markers were associated with anxiety markers at Exam2. Conclusion Short sessions of HRV-BFB training for a brief period of 5 weeks bring substantial benefits to autonomic markers and anxiety levels in young competitors. Here beneficial effects persisted for 12 weeks. Dissociated profiles of anxiety and cardiac autonomic adaptations shed new light on the role of the amygdala in heart-brain interactions after cardiac coherence training.

Description: by Karina T. Barretto, Calvin M. Swanson, Christopher L. Nguyen, Douglas S. Annis, Stephane J. Esnault, Deane F. Mosher, Mats W. Johansson Periostin, which is induced by interleukin (IL)-13, is an extracellular matrix (ECM) protein that supports α M β 2 integrin-mediated adhesion and migration of IL-5-stimulated eosinophils. Transforming growth factor (TGF)-β-induced protein (TGFBI) is a widely expressed periostin paralog known to support monocyte adhesion. Our objective was to compare eosinophil adhesion and migration on TGFBI and periostin in the presence of IL-5-family cytokines. Eosinophil adhesion after 1 h and random motility over 20 h in the presence of various concentrations of IL-5, IL-3, or granulocyte macrophage-colony stimulating factor (GM-CSF) were quantified in wells coated with various concentrations of TGFBI or periostin. Results were compared to video microscopy of eosinophils. Cytokine-stimulated eosinophils adhered equivalently well to TGFBI or periostin in a coating concentration-dependent manner. Adhesion was blocked by anti-α M β 2 and stimulated at the lowest concentration by GM-CSF. In the motility assay, periostin was more potent than TGFBI, the coating-concentration effect was bimodal, and IL-3 was the most potent cytokine. Video microscopy revealed that under the optimal coating condition of 5 μg/ml periostin, most eosinophils migrated persistently and were polarized and acorn-shaped with a ruffling forward edge and granules gathered together, in front of the nucleus. On 10 μg/ml periostin or TGFBI, more eosinophils adopted a flattened pancake morphology with dispersed granules and nuclear lobes, and slower migration. Conversion between acorn and pancake morphologies were observed. We conclude that TGFBI or periostin supports two modes of migration by IL-5 family cytokine-activated eosinophils. The rapid mode is favored by intermediate protein coatings and the slower by higher coating concentrations. We speculate that eosinophils move by haptotaxis up a gradient of adhesive ECM protein and then slow down to surveil the tissue.

Description: by Lemmy Schakel, Dieuwke S. Veldhuijzen, Henriët van Middendorp, Pieter Van Dessel, Jan De Houwer, Rafael Bidarra, Andrea W. M. Evers There is initial support for the effectiveness of approach-avoidance trainings in altering food-related health behaviors. Furthermore, outcome expectancies induced by verbal suggestions might optimize the effectiveness of these interventions, as shown in placebo research. The present study investigated the effectiveness of a gamified approach-avoidance training on food-related outcomes and whether verbal suggestions could strengthen those effects. A total of 120 participants were randomly assigned to 1 of 4 conditions: serious gaming only, verbal suggestions only, serious gaming combined with verbal suggestions, or a gaming control condition. Virtual food preference and food choice were assessed with a food choice task, with pairs differing in healthiness or in healthiness and attractiveness. Implicit food preference was assessed with an Implicit Association Test and food intake with a bogus taste test. Participants in both serious gaming conditions made healthier food choices for pairs differing in healthiness and attractiveness and had healthier implicit food preferences compared to gaming control. No effects were found on food intake. These findings provide the first preliminary support for the effects of a gamified approach-avoidance training on virtual food choice and implicit food preference. Future studies should further elucidate these effects, also in other health domains such as physical activity.

Description: by Juan M. Castellote, Markus Kofler Objective To provide a neurophysiological tool for assessing sensorimotor pathways, which may differ for those involving distal muscles in simple tasks from those involving distal muscles in a kinetic chain task, or proximal muscles in both. Methods We compared latencies and magnitudes of motor responses in a reaction time paradigm in a proximal (biceps brachii, BB) and a distal (first dorsal interosseous, FDI) muscle following electrical stimuli used as imperative signal (IS) delivered to the index finger. These stimuli were applied during different motor tasks: simple tasks involving either one muscle, e.g. flexing the elbow for BB (FLEX), or pinching a pen for FDI (PINCH); combined tasks engaging both muscles by pinching and flexing simultaneously (PINCH-FLEX). Stimuli were of varying intensity and occasionally elicited a startle response, and a StartReact effect. Results In BB, response latencies decreased gradually and response amplitudes increased progressively with increasing IS intensities for non-startling trials, while for trials containing startle responses, latencies were uniformly shortened and response amplitudes similarly augmented across all IS intensities in both FLEX and PINCH-FLEX. In FDI, response latencies decreased gradually and response amplitudes increased progressively with increasing IS intensities in both PINCH and PINCH-FLEX for non-startling trials, but, unlike in BB for the simple task, in PINCH for trials containing startle responses as well. In PINCH-FLEX, FDI latencies were uniformly shortened and amplitudes similarly increased across all stimulus intensities whenever startle signs were present. Conclusions Our results suggest the presence of different sensorimotor pathways supporting a dissociation between simple tasks that involve distal upper limb muscles (FDI in PINCH) from simple tasks involving proximal muscles (BB in FLEX), and combined tasks that engage both muscles (FDI and BB in PINCH-FLEX), all in accordance with differential importance in the control of movements by cortical and subcortical structures. Significance Simple assessment tools may provide useful information regarding the differential involvement of sensorimotor pathways in the control of both simple and combined tasks that engage proximal and distal muscles.

Description: by Yuki Ideno, Kunihiko Hayashi, Junko Nakajima-Shimada, Yoko Onizuka, Mikiko Kishi, Tomomi Ueno, Shigeto Uchiyama Equol is one of the most active soy isoflavones. When the association between soy food intake in daily life and health outcomes is examined in epidemiological studies, it is important to define the equol-producing status of each individual. However, few studies have assessed equol-producing status without a soy challenge test. To determine a robust cutoff criterion for equol producer classification in observational studies, we conducted a urinary isoflavone concentration survey in daily life among women. Furthermore, we examined the association between eating habits regarding soy foods and equol-producing status. A total of 4,412 participants were included in the analyses. Urinary isoflavones were analyzed using a high-performance liquid chromatography method. We examined the distribution of the log 10 equol/daidzein ratios, finding a mixture of two normal distributions, corresponding to equol producer and non-producer subpopulations. Applying a finite mixture model, we estimated the means, standard deviations, and mixing proportions of these two distributions. The estimation was carried out using the SAS NLIN procedure. The optimal cutoff point for the log 10 equol/daidzein ratio in the study population was determined to be −1.42, according to the estimated parameters of the mixture distribution. Based on this criterion, 1,830 (41.5%) of the participants were identified as equol producers. Compared with non-consumers of soy foods, consumers of soy foods had significantly higher odds of being equol producers. Using log 10 -transformed equol/daidzein ratios ≥ −1.42 to define equol producers among Japanese women is reasonable and suitable for determining equol-producing status in epidemiological studies. We found that soy food eating habits were associated with equol-producing status. Further investigation is required to evaluate associations between equol-producing status in daily life and health outcomes. The results of this study suggest the best cutoff point to use in the definition of equol-producing status in daily life.

Description: by Ehsan Mirzakhalili, Bogdan I. Epureanu, Eleni Gourgou We propose a mathematical and computational model that captures the stimulus-generated Ca 2+ transients in the C . elegans ASH sensory neuron. The rationale is to develop a tool that will enable a cross-talk between modeling and experiments, using modeling results to guide targeted experimental efforts. The model is built based on biophysical events and molecular cascades known to unfold as part of neurons' Ca 2+ homeostasis mechanism, as well as on Ca 2+ signaling events. The state of ion channels is described by their probability of being activated or inactivated, and the remaining molecular states are based on biochemically defined kinetic equations or known biochemical motifs. We estimate the parameters of the model using experimental data of hyperosmotic stimulus-evoked Ca 2+ transients detected with a FRET sensor in young and aged worms, unstressed and exposed to oxidative stress. We use a hybrid optimization method composed of a multi-objective genetic algorithm and nonlinear least-squares to estimate the model parameters. We first obtain the model parameters for young unstressed worms. Next, we use these values of the parameters as a starting point to identify the model parameters for stressed and aged worms. We show that the model, in combination with experimental data, corroborates literature results. In addition, we demonstrate that our model can be used to predict ASH response to complex combinations of stimulation pulses. The proposed model includes for the first time the ASH Ca 2+ dynamics observed during both "on" and "off" responses. This mathematical and computational effort is the first to propose a dynamic model of the Ca 2+ transients' mechanism in C . elegans neurons, based on biochemical pathways of the cell's Ca 2+ homeostasis machinery. We believe that the proposed model can be used to further elucidate the Ca 2+ dynamics of a key C . elegans neuron, to guide future experiments on C . elegans neurobiology, and to pave the way for the development of more mathematical models for neuronal Ca 2+ dynamics.

Description: by Brittney M. Donovan, Nichole L. Nidey, Elizabeth A. Jasper, Jennifer G. Robinson, Wei Bao, Audrey F. Saftlas, Kelli K. Ryckman Biomarkers commonly assessed in prenatal screening have been associated with a number of adverse perinatal and birth outcomes. However, it is not clear whether first trimester measurements of prenatal screening biomarkers are associated with subsequent risk of gestational diabetes mellitus (GDM). We aimed to systematically review and statistically summarize studies assessing the relationship between first trimester prenatal screening biomarker levels and GDM development. We comprehensively searched PubMed/MEDLINE, EMBASE, CINAHL, and Scopus (from inception through January 2018) and manually searched the reference lists of all relevant articles. We included original, published, observational studies examining the association of first trimester pregnancy associated plasma protein-A (PAPP-A) and/or free β-human chorionic gonadotropin (free β-hCG) levels with GDM diagnosis. Mean differences were calculated comparing PAPP-A and free β-hCG multiples of median (MoM) levels between women who developed GDM and those who did not and were subsequently pooled using two-sided random-effects models. Our meta-analysis of 13 studies on PAPP-A and nine studies on free β-hCG indicated that first trimester MoM levels for both biomarkers were lower in women who later developed GDM compared to women who remained normoglycemic throughout pregnancy (MD -0.17; 95% CI -0.24, -0.10; MD -0.04; 95% CI -0.07–0.01). There was no evidence for between-study heterogeneity among studies on free β-hCG (I 2 = 0%). A high level of between-study heterogeneity was detected among the studies reporting on PAPP-A (I 2 = 90%), but was reduced after stratifying by geographic location, biomarker assay method, and timing of GDM diagnosis. Our meta-analysis indicates that women who are diagnosed with GDM have lower first trimester levels of both PAPP-A and free β-hCG than women who remain normoglycemic throughout pregnancy. Further assessment of the predictive capacity of these biomarkers within large, diverse populations is needed.

Description: by Hsin-Hua Chen, Wen-Cheng Chao, Tsai-Ling Liao, Ching-Heng Lin, Der-Yuan Chen Objective To estimate the relative risk of autoimmune rheumatic diseases, including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), systemic sclerosis (SSc), Sjogren’s syndrome (SS), dermatomyositis (DM) and polymyositis (PM), among patients with palindromic rheumatism (PR) compared with non-PR individuals. Methods The study utilized 2003–2013 claims data from the Taiwanese National Health Insurance Research Database. We identified 4,421 cases of PR from 2007 to 2012 and randomly chose 44,210 non-PR individuals who matched (1:10) for age, sex and the year of index date without prior history of RA, SLE, SSc, SS, DM, or PM. After adjusting for age, sex, and the Charlson comorbidity index, we calculated the hazard ratios (HRs) with 95% confidence intervals (CIs) using the Cox proportional hazard model to quantify the risk of RA, SLE, SS, DM and PM in PR patients compared with that in matched non-PR individuals. Results Among the 4,421 patients with PR, 569 (12.87%) developed RA, 269 (6.08%) developed SS, 113 (2.56%) developed SLE, 5 (0.11%) developed SSc, 8 (0.18%) developed PM, and 1 (0.02%) developed DM. After adjusting for potential confounders, the patients with PR had an increased risk of RA (HR, 118.76; 95% CI, 89.81–157.04), SS (HR, 59.57; 95% CI, 43.87–80.88), SLE (HR, 51.56; 95% CI, 32.96–80.66) PM (HR, 57.38; 95% CI, 6.90–476.83), and SSc (HR, 13.42; 95% CI, 3.79–47.55) but not of DM (HR, 3.44; 95% CI, 0.34–34.59). Conclusion Patients with PR had an increased risk of developing RA, SS, SLE, PM, and SSc.

Description: Remote Sensing, Vol. 10, Pages 1181: Improved Albedo Estimates Implemented in the METRIC Model for Modeling Energy Balance Fluxes and Evapotranspiration over Agricultural and Natural Areas in the Brazilian Cerrado Remote Sensing doi: 10.3390/rs10081181 Authors: Bruno Silva Oliveira Elisabete Caria Moraes Marcos Carrasco-Benavides Gabriel Bertani Guilherme Augusto Verola Mataveli In this study we assessed METRIC (Mapping Evapotranspiration at high Resolution with Internalized Calibration) model performance to estimate energy balance fluxes and evapotranspiration (ET) in two heterogeneous landscapes in the Brazilian Cerrado, including fluxes and ET in both agricultural and natural vegetation. The estimates were evaluated by comparing them to flux tower data collected over sugarcane (USR site), woody savanna (PDG site) and stricto-sensu savanna (RECOR site) areas. The selection of the study years (2005–2007 for USR/PDG sites and 2011–2015 for RECOR site) was based on the availability of meteorological data (to be used as inputs in METRIC) and of flux tower data for energy balance fluxes and ET comparisons. The broadband albedo submodel was adjusted in order to improve Net Radiation estimates. For this adjustment, we applied at-surface solar radiation simulations obtained from the SMARTS2 model under different conditions of land elevation, precipitable water content and solar angles. We also tested the equivalence between the measured crop coefficient (Kc_ec) and the reference evapotranspiration fraction (ETrF or F), seeking to extrapolate from instantaneous to daily values of actual evapotranspiration (ETa). Surface albedo was underestimated by 10% at the USR site (showing a better performance for full crop coverage), by 15% at the PDG site (following the woody savanna dynamics pattern through dry and wet seasons) and was overestimated by 21% at the RECOR site. METRIC was effective in simulating the spatial and temporal variability of energy balance fluxes and ET over agricultural and natural vegetation in the Brazilian Cerrado, with errors within those reported in the literature. Net radiation (Rn) presented consistent results (coefficient of determination (R2) > 0.94) but it was overestimated by 8% and 9% in sugarcane and woody savanna, respectively. METRIC-derived ET estimates showed an agreement with ground data at USR and PDG sites (R2 > 0.88, root mean square error (RMSE) up to 0.87 mm day−1), but at the RECOR site, ET was overestimated by 14% (R2 = 0.96, mean absolute error (MAE) = 0.62 mm.day−1 and RMSE = 0.75 mm day−1). Surface energy balance fluxes and ET were marked by seasonality, with direct dependence on available energy, rainfall distribution, soil moisture and other parameters like albedo and NDVI.

Description: Remote Sensing, Vol. 10, Pages 1182: Detection of Frozen Soil Using Sentinel-1 SAR Data Remote Sensing doi: 10.3390/rs10081182 Authors: Nicolas Baghdadi Hassan Bazzi Mohammad El Hajj Mehrez Zribi The objective of this paper is to evaluate the potential of Sentinel-1 Synthetic Aperture Radar “SAR” data (C-band) for monitoring agricultural frozen soils. First, investigations were conducted from simulated radar signal data using a SAR backscattering model combined with a dielectric mixing model. Then, Sentinel-1 images acquired at a study site near Paris, France were analyzed using temperature data to investigate the potential of the new Sentinel-1 SAR sensor for frozen soil mapping. The results show that the SAR backscattering coefficient decreases when the soil temperature drops below 0 °C. This decrease in signal is the most important for temperatures that ranges between 0 and −5 °C. A difference of at least 2 dB is observed between unfrozen soils and frozen soils. This difference increases under freezing condition when the temperature at the image acquisition date decreases. In addition, results show that the potential of the C-band radar signal for the discrimination of frozen soils slightly decreases when the soil moisture decreases (simulated data were used with soil moisture contents of 20 and 30 vol%). The difference between the backscattering coefficient of unfrozen soil and the backscattering coefficient of frozen soil decreases by approximately 1 dB when the soil moisture decreases from 30 to 20 vol%). Finally, the results show that both VV and VH allow a good detection of frozen soils but the sensitivity of VH is higher by approximately 1.5 dB. In conclusion, this study shows that the difference between a reference image acquired without freezing and an image acquired under freezing conditions is a good tool for detecting frozen soils.

Description: Sensors, Vol. 18, Pages 2436: Bio-Inspired Covert Active Sonar Strategy Sensors doi: 10.3390/s18082436 Authors: Jiajia Jiang Xianquan Wang Fajie Duan Chunyue Li Xiao Fu Tingting Huang Lingran Bu Ling Ma Zhongbo Sun The covertness of the active sonar is a very important issue and the sonar signal waveform design problem was studied to improve covertness of the system. Many marine mammals produce call pulses for communication and echolocation, and existing interception systems normally classify these biological signals as ocean noise and filter them out. Based on this, a bio-inspired covert active sonar strategy was proposed. The true, rather than man-made sperm whale, call pulses were used to serve as sonar waveforms so as to ensure the camouflage ability of sonar waveforms. A range and velocity measurement combination (RVMC) was designed by using two true sperm whale call pulses which had excellent range resolution (RR) and large Doppler tolerance (DT). The range and velocity estimation methods were developed based on the RVMC. In the sonar receiver, the correlation technology was used to confirm the start and end time of sonar signals and their echoes, and then based on the developed range and velocity estimation method, the range and velocity of the underwater target were obtained. Then, the RVMC was embedded into the true sperm whale call-train to improve the camouflage ability of the sonar signal-train. Finally, experiment results were provided to verify the performance of the proposed method.

Description: Sensors, Vol. 18, Pages 2435: Quantitative Contact-Less Estimation of Energy Expenditure from Video and 3D Imagery Sensors doi: 10.3390/s18082435 Authors: Gregor Koporec Goran Vučković Radoje Milić Janez Perš Measurement of energy expenditure is an important tool in sport science and medicine, especially when trying to estimate the extent and intensity of physical activity. However, most approaches still rely on sensors or markers, placed directly on the body. In this paper, we present a novel approach using a fully contact-less, fully automatic method, that relies on computer vision algorithms and widely available and inexpensive imaging sensors. We rely on the estimation of the optical and scene flow to calculate Histograms of Oriented Optical Flow (HOOF) descriptors, which we subsequently augment with the Histograms of Absolute Flow Amplitude (HAFA). Descriptors are fed into regression model, which allows us to estimate energy consumption, and to a lesser extent, the heart rate. Our method has been tested both in lab environment and in realistic conditions of a sport match. Results confirm that these energy expenditures could be derived from purely contact-less observations. The proposed method can be used with different modalities, including near infrared imagery, which extends its future potential.

Description: Sensors, Vol. 18, Pages 2434: Smart Portable Devices Suitable for Cultural Heritage: A Review Sensors doi: 10.3390/s18082434 Authors: Federica Valentini Andrea Calcaterra Simonetta Antonaroli Maurizio Talamo This article reviews recent portable sensor technologies to apply in the Cultural Heritage (CH) fields. The review has been prepared in the form of a retrospective description of the sensor’s history and technological evolution, having: new nanomaterials for transducers, miniaturized, portable and integrated sensors, the wireless transmission of the analytical signals, ICT_Information Communication Technology and IoT_Internet of Things to apply to the cultural heritage field. In addition, a new trend of movable tattoo sensors devices is discussed, referred to in situ analysis, which is especially important when scientists are in the presence of un-movable and un-tangible Cultural Heritage and Art Work objects. The new proposed portable contact sensors (directly applied to art work objects and surfaces) are non-invasive and non-destructive to the different materials and surfaces of which cultural heritage is composed.

Description: Sensors, Vol. 18, Pages 2433: Multi-Camera Imaging System for UAV Photogrammetry Sensors doi: 10.3390/s18082433 Authors: Damian Wierzbicki In the last few years, it has been possible to observe a considerable increase in the use of unmanned aerial vehicles (UAV) equipped with compact digital cameras for environment mapping. The next stage in the development of photogrammetry from low altitudes was the development of the imagery data from UAV oblique images. Imagery data was obtained from side-facing directions. As in professional photogrammetric systems, it is possible to record footprints of tree crowns and other forms of the natural environment. The use of a multi-camera system will significantly reduce one of the main UAV photogrammetry limitations (especially in the case of multirotor UAV) which is a reduction of the ground coverage area, while increasing the number of images, increasing the number of flight lines, and reducing the surface imaged during one flight. The approach proposed in this paper is based on using several head cameras to enhance the imaging geometry during one flight of UAV for mapping. As part of the research work, a multi-camera system consisting of several cameras was designed to increase the total Field of View (FOV). Thanks to this, it will be possible to increase the ground coverage area and to acquire image data effectively. The acquired images will be mosaicked in order to limit the total number of images for the mapped area. As part of the research, a set of cameras was calibrated to determine the interior orientation parameters (IOPs). Next, the method of image alignment using the feature image matching algorithms was presented. In the proposed approach, the images are combined in such a way that the final image has a joint centre of projections of component images. The experimental results showed that the proposed solution was reliable and accurate for the mapping purpose. The paper also presents the effectiveness of existing transformation models for images with a large coverage subjected to initial geometric correction due to the influence of distortion.

Description: Sensors, Vol. 18, Pages 2432: A Terahertz CMOS V-Shaped Patch Antenna with Defected Ground Structure Sensors doi: 10.3390/s18082432 Authors: Hyeongjin Kim Wonseok Choe Jinho Jeong In this paper, a V-shaped patch antenna with defected ground structure is proposed at terahertz to overcome the limited performance of a standard complementary metal-oxide semiconductor (CMOS) patch antenna consisting of several metal layers and very thin interdielectric layers. The proposed V-shaped patch with slots allows the increased radiation resistance and broadband performance. In addition, the patch resonating at different frequency from the V-shaped patch is stacked on the top to broaden the impedance-matching bandwidth. More importantly, the slots are formed in the ground plane, which is called the defected ground structure, to further increase the radiation resistance and thus improve the bandwidth and efficiency. It is verified from electromagnetic simulations that the leakage waves from the defected ground can enhance the antenna directivity and gain by coherently interfering with the topside radiation. The proposed on-chip antenna is fabricated using a standard 65 nm CMOS process. The on-wafer measurement shows very wide bandwidth in input reflection coefficient (<−10 dB), greater than 28.7% from 240 to >320 GHz. The measured peak gain was as high as 5.48 dBi at 295 GHz. To the best of the authors’ knowledge, these results belong to the best performance among the terahertz CMOS on-chip antennas without using additional components or processes such as dielectric resonators, lens, or substrate thinning.

Description: Sensors, Vol. 18, Pages 2430: Relative Pose Based Redundancy Removal: Collaborative RGB-D Data Transmission in Mobile Visual Sensor Networks Sensors doi: 10.3390/s18082430 Authors: Xiaoqin Wang Y. Ahmet Şekercioğlu Tom Drummond Vincent Frémont Enrico Natalizio Isabelle Fantoni In this paper, the Relative Pose based Redundancy Removal (RPRR) scheme is presented, which has been designed for mobile RGB-D sensor networks operating under bandwidth-constrained operational scenarios. The scheme considers a multiview scenario in which pairs of sensors observe the same scene from different viewpoints, and detect the redundant visual and depth information to prevent their transmission leading to a significant improvement in wireless channel usage efficiency and power savings. We envisage applications in which the environment is static, and rapid 3D mapping of an enclosed area of interest is required, such as disaster recovery and support operations after earthquakes or industrial accidents. Experimental results show that wireless channel utilization is improved by 250% and battery consumption is halved when the RPRR scheme is used instead of sending the sensor images independently.

Description: Sensors, Vol. 18, Pages 2439: HemoKinect: A Microsoft Kinect V2 Based Exergaming Software to Supervise Physical Exercise of Patients with Hemophilia Sensors doi: 10.3390/s18082439 Authors: Fernando Mateo Emilio Soria-Olivas Juan Carrasco Santiago Bonanad Felipe Querol Sofía Pérez-Alenda Patients with hemophilia need to strictly follow exercise routines to minimize their risk of suffering bleeding in joints, known as hemarthrosis. This paper introduces and validates a new exergaming software tool called HemoKinect that intends to keep track of exercises using Microsoft Kinect V2’s body tracking capabilities. The software has been developed in C++ and MATLAB. The Kinect SDK V2.0 libraries have been used to obtain 3D joint positions from the Kinect color and depth sensors. Performing angle calculations and center-of-mass (COM) estimations using these joint positions, HemoKinect can evaluate the following exercises: elbow flexion/extension, knee flexion/extension (squat), step climb (ankle exercise) and multi-directional balance based on COM. The software generates reports and progress graphs and is able to directly send the results to the physician via email. Exercises have been validated with 10 controls and eight patients. HemoKinect successfully registered elbow and knee exercises, while displaying real-time joint angle measurements. Additionally, steps were successfully counted in up to 78% of the cases. Regarding balance, differences were found in the scores according to the difficulty level and direction. HemoKinect supposes a significant leap forward in terms of exergaming applicability to rehabilitation of patients with hemophilia, allowing remote supervision.

Description: Sensors, Vol. 18, Pages 2428: Narrowband Characterization of Near-Ground Radio Channel for Wireless Sensors Networks at 5G-IoT Bands Sensors doi: 10.3390/s18082428 Authors: Hicham Klaina Ana Vazquez Alejos Otman Aghzout Francisco Falcone In this contribution, a narrowband radio channel model is proposed for rural scenarios in which the radio link operates under near-ground conditions for application in wireless sensor networks dedicated to smart agriculture. The received power attenuation was measured for both transmitter and receiver antennas placed at two different heights above ground: 0.2 and 0.4 m. Three frequency ranges, proposed for future 5G-IoT use case in agriculture, were chosen: 868 MHz, 2.4 GHz and 5.8 GHz. Three ground coverings were tested in a rural scenario: soil, short and tall grass fields. The path loss was then estimated as dependent of the radio link range and a three-slope log-normal path loss model was tailored. Results are explained in terms of the first Fresnel zone obstruction. Commercial Zigbee sensor nodes operating at 2.4 GHz were used in a second experiment to estimate the link quality from the experimental Radio Signal Strength Indicator (RSSI) received values. Two sensor nodes were placed at the same elevation above ground as in the previous experiment, only for short grass field case. The Quality of Service performance was determined in terms of theoretical bit error rate achieved for different digital modulations—BPSK, 8PSK and 16QAM—concluding remarkable results for an obstructed radio link.

Description: Sensors, Vol. 18, Pages 2429: A PUF- and Biometric-Based Lightweight Hardware Solution to Increase Security at Sensor Nodes Sensors doi: 10.3390/s18082429 Authors: Rosario Arjona Miguel Ángel Prada-Delgado Javier Arcenegui Iluminada Baturone Security is essential in sensor nodes which acquire and transmit sensitive data. However, the constraints of processing, memory and power consumption are very high in these nodes. Cryptographic algorithms based on symmetric key are very suitable for them. The drawback is that secure storage of secret keys is required. In this work, a low-cost solution is presented to obfuscate secret keys with Physically Unclonable Functions (PUFs), which exploit the hardware identity of the node. In addition, a lightweight fingerprint recognition solution is proposed, which can be implemented in low-cost sensor nodes. Since biometric data of individuals are sensitive, they are also obfuscated with PUFs. Both solutions allow authenticating the origin of the sensed data with a proposed dual-factor authentication protocol. One factor is the unique physical identity of the trusted sensor node that measures them. The other factor is the physical presence of the legitimate individual in charge of authorizing their transmission. Experimental results are included to prove how the proposed PUF-based solution can be implemented with the SRAMs of commercial Bluetooth Low Energy (BLE) chips which belong to the communication module of the sensor node. Implementation results show how the proposed fingerprint recognition based on the novel texture-based feature named QFingerMap16 (QFM) can be implemented fully inside a low-cost sensor node. Robustness, security and privacy issues at the proposed sensor nodes are discussed and analyzed with experimental results from PUFs and fingerprints taken from public and standard databases.

Description: Sustainability, Vol. 10, Pages 2633: Scenario-Based Simulation of Tianjin City Using a Cellular Automata–Markov Model Sustainability doi: 10.3390/su10082633 Authors: Ruci Wang Hao Hou Yuji Murayama Rapid urbanization is occurring throughout China, especially in megacities. Using a land use model to obtain future land use/cover conditions is an essential method to prevent chaotic urban sprawl and imbalanced development. This study utilized historical Landsat images to create land use/cover maps to predict the land use/cover changes of Tianjin city in 2025 and 2035. The cellular automata–Markov (CA–Markov) model was applied in the simulation under three scenarios: the environmental protection scenario (EPS), crop protection scenario (CPS), and spontaneous scenario (SS). The model achieved a kappa value of 86.6% with a figure of merit (FoM) of 12.18% when compared to the empirical land use/cover map in 2015. The results showed that the occupation of built-up areas increased from 29.13% in 2015 to 38.68% (EPS), 36.18% (CPS), and 47.94% (SS) in 2035. In this context, current urbanization would bring unprecedented stress on agricultural resources and forest ecosystems, which could be attenuated by implementing protection policies along with decelerating urban expansion. The findings provide valuable information for urban planners to achieve sustainable development goals.

Description: Sustainability, Vol. 10, Pages 2629: Pathways to Coastal Resiliency: The Adaptive Gradients Framework Sustainability doi: 10.3390/su10082629 Authors: Elisabeth M. Hamin Yaser Abunnasr Max Roman Dilthey Pamela K. Judge Melissa A. Kenney Paul Kirshen Thomas C. Sheahan Don J. DeGroot Robert L. Ryan Brain G. McAdoo Leonard Nurse Jane A. Buxton Ariana E. Sutton-Grier Elizabeth A. Albright Marielos Arlen Marin Rebecca Fricke Current and future climate-related coastal impacts such as catastrophic and repetitive flooding, hurricane intensity, and sea level rise necessitate a new approach to developing and managing coastal infrastructure. Traditional “hard” or “grey” engineering solutions are proving both expensive and inflexible in the face of a rapidly changing coastal environment. Hybrid solutions that incorporate natural, nature-based, structural, and non-structural features may better achieve a broad set of goals such as ecological enhancement, long-term adaptation, and social benefits, but broad consideration and uptake of these approaches has been slow. One barrier to the widespread implementation of hybrid solutions is the lack of a relatively quick but holistic evaluation framework that places these broader environmental and societal goals on equal footing with the more traditional goal of exposure reduction. To respond to this need, the Adaptive Gradients Framework was developed and pilot-tested as a qualitative, flexible, and collaborative process guide for organizations to understand, evaluate, and potentially select more diverse kinds of infrastructural responses. These responses would ideally include natural, nature-based, and regulatory/cultural approaches, as well as hybrid designs combining multiple approaches. It enables rapid expert review of project designs based on eight metrics called “gradients”, which include exposure reduction, cost efficiency, institutional capacity, ecological enhancement, adaptation over time, greenhouse gas reduction, participatory process, and social benefits. The framework was conceptualized and developed in three phases: relevant factors and barriers were collected from practitioners and experts by survey; these factors were ranked by importance and used to develop the initial framework; several case studies were iteratively evaluated using this technique; and the framework was finalized for implementation. The article presents the framework and a pilot test of its application, along with resources that would enable wider application of the framework by practitioners and theorists.

Description: Sustainability, Vol. 10, Pages 2627: Photovoltaic Power Forecasting Based on EEMD and a Variable-Weight Combination Forecasting Model Sustainability doi: 10.3390/su10082627 Authors: Hui Wang Jianbo Sun Weijun Wang It is widely considered that solar energy will be one of the most competitive energy sources in the future, and solar energy currently accounts for high percentages of power generation in developed countries. However, its power generation capacity is significantly affected by several factors; therefore, accurate prediction of solar power generation is necessary. This paper proposes a photovoltaic (PV) power generation forecasting method based on ensemble empirical mode decomposition (EEMD) and variable-weight combination forecasting. First, EEMD is applied to decompose PV power data into components that are then combined into three groups: low-frequency, intermediate-frequency, and high-frequency. These three groups of sequences are individually predicted by the variable-weight combination forecasting model and added to obtain the final forecasting result. In addition, the design of the weights for combination forecasting was studied during the forecasting process. The comparison in the case study indicates that in PV power generation forecasting, the prediction results obtained by the individual forecasting and summing of the sequences after the EEMD are better than those from direct prediction. In addition, when the single prediction model is converted to a variable-weight combination forecasting model, the prediction accuracy is further improved by using the optimal weights.

Description: Sustainability, Vol. 10, Pages 2622: A Rough Multi-Criteria Decision-Making Approach for Sustainable Supplier Selection under Vague Environment Sustainability doi: 10.3390/su10082622 Authors: Huiyun Lu Shaojun Jiang Wenyan Song Xinguo Ming With the growing awareness of environmental and social issues, sustainable supply chain management (SSCM) has received considerable attention both in academia and industry. Supplier selection plays an important role in the successful implementation of sustainable supply chain management, because it can influence the performance of SSCM. Sustainable supplier selection is a typical multi-criteria decision-making problem involving subjectivity and vagueness. Although some previous researches of supplier selection use fuzzy approaches to deal with vague information, it has been criticized for requiring much priori information and inflexibility in manipulating vagueness. Moreover, the previous methods often omit the environmental and social evaluation criteria in the supplier selection. To manipulate these problems, a new approach based on the rough set theory and ELECTRE (ELimination Et Choix Traduisant la REalité) is developed in this paper. The novel approach integrates the strength of rough set theory in handling vagueness without much priori information and the merit of ELECTRE in modeling multi-criteria decision-making problem. Finally, a case study of sustainable supplier selection for solar air-conditioner manufacturer is provided to demonstrate the application and potential of the approach.

Description: Sustainability, Vol. 10, Pages 2624: Economic Transition and Changing Location of Manufacturing Industry in China: A Study of the Yangtze River Delta Sustainability doi: 10.3390/su10082624 Authors: Jiawei Wu Yehua Dennis Wei Qizhai Li Feng Yuan Industrial restructuring is widely considered an important force in regional economic growth and sustainable development. With increased globalization and economic transition, a dramatic industrial restructuring has been taking place in China. Applying geographically weighted shift-share model (GW-SSM) and geographically and temporally weighted regression model (GTWR), we analyze (re)location dynamics and determinants of the manufacturing industry in the Yangtze River Delta (YRD) from 1999 to 2013, with particular attention to the implications of economic transition and institutional restructuring. We find that high-tech and capital-intensive manufacturing industries agglomerated in coastal cities, while labor-intensive and resource-based sectors have become spatially more dispersed to peripheral areas. We also find that the development of service and high-tech industries, rising labor costs, and more strict environmental regulations have facilitated the geographic dispersion of labor- and pollution-intensive industries. Moreover, regions with advantages in intermediate goods, preferential policies, and urbanization economies are attractive to capital- and technology-intensive manufacturing industries. Our research suggests that development policies should be tailored to specific regions to promote local production and innovative networks and make manufacturing industries more competitive.

Description: Sustainability, Vol. 10, Pages 2621: Adoption of Conservation Tillage on the Semi-Arid Loess Plateau of Northwest China Sustainability doi: 10.3390/su10082621 Authors: Qingfeng Han Kadambot H. M. Siddique Fengmin Li Conservation tillage is an important approach to prevent water loss and soil erosion and promote soil fertility that has been adopted widely throughout the world. However, despite promotion of the benefits of conservation tillage, obstacles are still encountered in some regions. A survey of 385 farmer households in the semi-arid Loess Plateau of China was conducted to assess the adoption of conservation tillage (ACT). This investigation was located in two counties that have run conservation tillage demonstrations with wheat for at least eight years. A binary logistic regression model was used to quantify the factors determining whether or not farmers adopt conservation tillage. Farmer’s education level, the influence of training, and field demonstrations by agricultural departments had significant positive effects on ACT. Although the adoption rate of conservation tillage in this paper was very high (89%), farmers were reluctant to continue practicing conservation tillage based on their experiences, which is contrary to the expectations of the government. The area available for planting winter wheat and the number of arable plots per household also had significant positive effects on ACT. However, the total cultivated area of land per household had a significant negative impact on ACT. Farmer awareness of conservation tillage technology, the distance from a farmer’s house to the nearest agricultural market, and the size of the active labor force in the family had significant negative impacts on ACT. These results will help in the development of more effective and targeted policies to improve the sustainability of farming systems on the semi-arid Loess Plateau.

Description: Sustainability, Vol. 10, Pages 2620: Transition towards Energy Efficiency: Developing the Nigerian Building Energy Efficiency Code Sustainability doi: 10.3390/su10082620 Authors: Susanne Geissler Doris Österreicher Ene Macharm In Nigeria, there is an estimated deficit of 17 million housing units. Power supply is insufficient, and the electricity supply for about 60 million Nigerians relies on private generators, causing noise, pollution, and high expenditures for mainly imported fuel. Altogether, current challenges clearly demonstrate the need for effective energy efficiency policies targeting also the building sector. The Nigerian Energy Support Program began in 2013, among others, with the objective being to support the Nigerian Government in developing the Nigerian Building Energy Efficiency Code. This paper presents two preparatory activities carried out in order to come up with suggestions for a legal framework well suited for the situation on the ground: the Case Study Building Analysis carried out in collaboration with a Nigerian developer and the Nigerian Building Energy Efficiency Guideline, elaborated together with stakeholders. The results of preparatory activities pointed out that the code must put emphasis on climate adaptive design and must define requirements and procedures in a clear and simple way to allow for effective enforcement. Only then can energy-efficient mass housing be feasible in Nigeria. The paper concludes with a description of the Nigerian Building Energy Efficiency Code (BEEC), officially approved and launched by the Federal Minister of Power, Works and Housing on 29 August 2017.

Description: Sustainability, Vol. 10, Pages 2619: Are the World-Leading Primary Silver Mines Exhausting? Sustainability doi: 10.3390/su10082619 Authors: Marcello Ruberti Stefania Massari Silver is one of the rarest metals in nature and certainly among the most used ones, not only as precious good for financial investments but also for many industrial critical applications. Because it would be very difficult to analyze the situation regarding the future global availability of silver as a commodity in general, due to the necessity of a large amount of data which are hardly available, we have focused only on the mining production of primary silver. In particular, the study regards the activities of a sample consisting of twelve of the world top fifteen leading primary silver mines, which represent the 54% of the worldwide primary silver production and 16% of the global silver mining production. We have investigated the related exploitation state and trends of these twelve sites by elaborating their last ten-years statistics on silver production, ore grades, resources and reserves. The findings of this study, in short, are that the cumulative average silver ore grades, both in extracted mineral, resources and reserves, of the above selected mines, have decreased and also that the new mining fields (Dukat, San Bartolomé, Pirquitas, Saucito) have lower silver content indexes than the older ones.

Description: Water, Vol. 10, Pages 989: Computational Study of a Vertical Plunging Jet into Still Water Water doi: 10.3390/w10080989 Authors: Zegao Yin Qianqian Jia Yuan Li Yanxu Wang Dejun Yang The behavior of a vertical plunging jet was numerically investigated using the coupled Level Set and Volume of Fluid method. The computational results were in good agreement with the experimental results reported in the related literature. Vertical plunging jet characteristics, including the liquid velocity field, air void fraction, and turbulence kinetic energy, were explored by varying the distance between the nozzle exit and the still water level. It was found that the velocity at the nozzle exit plays an unimportant role in the shape and size of ascending bubbles. A modified prediction equation between the centerline velocity ratio and the axial distance ratio was developed using the data of the coupled Level Set and Volume of Fluid method, and it showed a better predicting ability than the Level Set and Mixture methods. The characteristics of turbulence kinetic energy, including its maximum value location and its radial and vertical distribution, were also compared with that of submerged jets.

Description: Water, Vol. 10, Pages 984: Vulnerability Analysis of the Venetian Littoral and Adopted Mitigation Strategy Water doi: 10.3390/w10080984 Authors: Piero Ruol Luca Martinelli Chiara Favaretto This paper discusses the key aspects of the recent Coastal Plan of the Veneto Region (IT). Its aim is to propose a single mitigation strategy for coastal erosion that is valid for the whole Veneto Region, and possibly elsewhere, as well as a method to assign a priority level to any action. The suggested mitigation action against erosion depends on urbanization level, beach width, as well as cross-shore and long-shore sediment transport. The criterion used to give a priority level to mitigation actions is based on a vulnerability index that takes into account erosive tendency, existing coastal flooding hazards, coast value, environmental relevance, tourist pressure, urbanization level, the presence of production activities, and cultural heritage. A sample case featuring the littoral of Rosolina is also provided and includes a site description, the sediment budget, critical issues and possible mitigation measures.

Description: Algorithms, Vol. 11, Pages 112: A Novel Parallel Auto-Encoder Framework for Multi-Scale Data in Civil Structural Health Monitoring Algorithms doi: 10.3390/a11080112 Authors: Ruhua Wang Ling Li Jun Li In this paper, damage detection/identification for a seven-storey steel structure is investigated via using the vibration signals and deep learning techniques. Vibration characteristics, such as natural frequencies and mode shapes are captured and utilized as input for a deep learning network while the output vector represents the structural damage associated with locations. The deep auto-encoder with sparsity constraint is used for effective feature extraction for different types of signals and another deep auto-encoder is used to learn the relationship of different signals for final regression. The existing SAF model in a recent research study for the same problem processed all signals in one serial auto-encoder model. That kind of models have the following difficulties: (1) the natural frequencies and mode shapes are in different magnitude scales and it is not logical to normalize them in the same scale in building the models with training samples; (2) some frequencies and mode shapes may not be related to each other and it is not fair to use them for dimension reduction together. To tackle the above-mentioned problems for the multi-scale dataset in SHM, a novel parallel auto-encoder framework (Para-AF) is proposed in this paper. It processes the frequency signals and mode shapes separately for feature selection via dimension reduction and then combine these features together in relationship learning for regression. Furthermore, we introduce sparsity constraint in model reduction stage for performance improvement. Two experiments are conducted on performance evaluation and our results show the significant advantages of the proposed model in comparison with the existing approaches.

Description: Diversity, Vol. 10, Pages 75: Kuschelysius, a New Alpine Genus of Eugnomine Weevil (Coleoptera: Curculionidae: Curculioninae) from New Zealand Diversity doi: 10.3390/d10030075 Authors: Samuel D. J. Brown Richard A. B. Leschen Kuschelysiusnew genus is described for four species, K. hollowayaenew species, K. durusnew species, K. verbalisnew species and K. nitensnew species, which are found in alpine regions along the length of the South Island of New Zealand. The genus most closely resembles members of the genus Eugnomus but is distinguished from them by the presence of a small pair of post-ocular tubercles and by having appressed scales on the dorsal surfaces. Some members of Kuschelysius appear to be flight-capable with well-developed hindwings, while others have reduced hindwings and are presumably flightless. Many specimens have been collected from the flowers of Dracophyllum traversii, Celmisia and other alpine plants, and the guts of examined specimens contained pollen. We hypothesise that the species of Kuschelysius are pollinators of the New Zealand alpine flora.

Description: Water, Vol. 10, Pages 982: Multivariate Chemometric Analysis of Membrane Fouling Patterns in Biofilm Ceramic Membrane Bioreactor Water doi: 10.3390/w10080982 Authors: Olga Kulesha Zakhar Maletskyi Harsha Ratnaweera Membrane fouling highly limits the development of Membrane bioreactor technology (MBR), which is among the key solutions to water scarcity. The current study deals with the determination of the fouling propensity of filtered biomass in a pilot-scale biofilm membrane bioreactor to enable the prediction of fouling intensity. The system was designed to treat domestic wastewater with the application of ceramic microfiltration membranes. Partial least squares regression analysis of the data obtained during the long-term operation of the biofilm-MBR (BF-MBR) system demonstrated that Mixed liquor suspended solids (MLSS), diluted sludge volume index (DSVI), chemical oxygen demand (COD), and their slopes are the most significant for the estimation and prediction of fouling intensity, while normalized permeability and its slope were found to be the most reliable fouling indicators. Three models were derived depending on the applied operating conditions, which enabled an accurate prediction of the fouling intensities in the system. The results will help to prevent severe membrane fouling via the change of operating conditions to prolong the effective lifetime of the membrane modules and to save energy and resources for the maintenance of the system.

Description: Water, Vol. 10, Pages 983: The Mechanical Properties of High Strength Reinforced Cured-in-Place Pipe (CIPP) Liner Composites for Urban Water Infrastructure Rehabilitation Water doi: 10.3390/w10080983 Authors: Hyun Wook Ji Sung Soo Yoo Jonghoon Kim Dan Daehyun Koo Most urban areas in the world have water infrastructure systems, including the buried sewer and water pipelines, which are assessed as in need of extensive rehabilitation. Deterioration by many other factors affects structural integrity. Trenchless technologies such as Cured-in-Place Pipe (CIPP) are now applied in numerous projects while minimizing disturbance in an urban environment. The main purpose of this study is to develop a high strength CIPP material using various composite materials (e.g., glass fiber, carbon fiber, polyester felt, unsaturated polyester resin, and others). Composite samples were made of the materials and tested using three-point bend apparatus to find mechanical properties, which include the flexural modulus, strength, and deflection. A composite combination with glass fibers with thin felt layers shows the best results in mechanical properties. Flexural modulus is a key factor for CIPP liner thickness design. Glass fiber composite yields between four and nine times higher values than the minimum value specified in the American Society for Testing and Materials (ASTM) F1216. This study provides a fundamental baseline for high strength CIPP liners that are capable of using conventional curing technologies.

Description: Sustainability, Vol. 10, Pages 2617: Exploring Twitter for CSR Disclosure: Influence of CEO and Firm Characteristics in Latin American Companies Sustainability doi: 10.3390/su10082617 Authors: Yuli Marcela Suárez-Rico Mauricio Gómez-Villegas María Antonia García-Benau Social networks, which are characterised by accessibility and interactivity, offer great potential for dialogue between companies and stakeholders, for example as platforms for publishing information on aspects of corporate social responsibility (CSR). In this paper, we use a synthetic index to analyse levels of CSR disclosure via Twitter, and identify explanatory variables of this disclosure by studying the demographic characteristics of the Chief Executive Officer (CEO) and of the company. This synthetic index was based on data for 93 companies located in the four countries of the Pacific Alliance (Chile, Colombia, Mexico, and Peru), using categories based on the 2016 Global Reporting Initave (GRI) Standards. The tweets were compiled during a period of two months in 2017, immediately before and after the publication of each CSR report. The synthetic index was taken as the dependent variable and used as the basis for multivariate regression analysis to identify the relationship between the level of CSR disclosure on Twitter and the characteristics of the firm and its CEO. The results obtained show that firms operating in environmentally-sensitive industries present higher levels of CSR disclosure on Twitter than those in other sectors. By country of origin, the Colombian and Chilean companies offered higher levels of disclosure than those in Mexico and Peru. The regression analysis revealed a positive relationship between the firm operating in a sensitive industry and its level of CSR disclosure on Twitter, and an inverse relationship between the latter variable and the tenure of the CEO.

Description: Algorithms, Vol. 11, Pages 113: Improved Parameter Identification Method for Envelope Current Signals Based on Windowed Interpolation FFT and DE Algorithm Algorithms doi: 10.3390/a11080113 Authors: Xiangfeng Su Huaiqing Zhang Lin Chen Ling Qin Lili Yu Envelope current signals are increasingly emerging in power systems, and their parameter identification is particularly necessary for accurate measurement of electrical energy. In order to analyze the envelope current signal, the harmonic parameters, as well as the envelope parameters, need to be calculated. The interpolation fast Fourier transform (FFT) is a widely used approach which can estimate the signal frequency with high precision, but it cannot calculate the envelope parameters of the signal. Therefore, this paper proposes an improved method based on windowed interpolation FFT (WIFFT) and differential evolution (DE). The amplitude and phase parameters obtained through WIFFT and the envelope parameters estimated by the envelope analysis are optimized using the DE algorithm, which makes full use of the performance advantage of DE. The simulation results show that the proposed method can improve the accuracy of the harmonic parameters and the envelope parameter significantly. In addition, it has good anti-noise ability and high precision.

Description: Atmosphere, Vol. 9, Pages 293: The Impact of Mount Washington on the Height of the Boundary Layer and the Vertical Structure of Temperature and Moisture Atmosphere doi: 10.3390/atmos9080293 Authors: Eric Kelsey Adriana Bailey Georgia Murray Discrimination of the type of air mass along mountain slopes can be a challenge and is not commonly performed, but is critical for identifying factors responsible for influencing montane weather, climate, and air quality. A field campaign to measure air mass type and transitions on the summit of Mount Washington, New Hampshire, USA was performed on 19 August 2016. Meteorological observations were taken at the summit and at several sites along the east and west slopes. Ozone concentrations were measured at the summit and on the valley floor. Additionally, water vapor stable isotopes were measured from a truck that drove up and down the Mount Washington Auto Road concurrent with radiosonde launches that profiled the free atmosphere. This multivariate perspective revealed thermal, moisture, and air mass height differences among the free atmosphere, leeward, and windward mountain slopes. Both thermally and mechanically forced upslope flows helped shape these differences by altering the height of the boundary layer with respect to the mountain surface. Recommendations for measurement strategies hoping to develop accurate observational climatologies of air mass exposure in complex terrain are discussed and will be important for evaluating elevation-dependent warming and improving forecasting for weather and air quality.

Description: Atmosphere, Vol. 9, Pages 292: A Mechanism of the Interdecadal Changes of the Global Low-Frequency Oscillation Atmosphere doi: 10.3390/atmos9080292 Authors: Ruowen Yang Quanliang Chen Yuyun Liu Lin Wang Based on the National Center for Environmental Prediction/National Center for Atmospheric Research reanalysis dataset from 1948 to 2009, this study reveals that global low-frequency oscillation features two major temporal bands. One is a quasi-60-day period known as the intraseasonal oscillation (ISO), and the other is a quasi-15-day period known as the quasi-biweekly oscillation (QBWO). After the mid-1970s, both the ISO and QBWO become intensified and more active, and these changes are equivalently barotropic. The primitive barotropic equations are adopted to study the involved mechanism. It reveals that the e-folding time of the least stable modes of both the ISO and QWBO becomes shorter if the model is solved under the atmospheric basic state after the mid-1970s than if solved under the basic state before the mid-1970s. This result suggests that the atmospheric basic flow after the mid-1970s facilitates a more rapid growth of the ISO and QBWO, and thereby an intensification of the low-frequency oscillations at the two bands.

Description: Atmosphere, Vol. 9, Pages 291: A Statistical Parameter Correction Technique for WRF Medium-Range Prediction of Near-Surface Temperature and Wind Speed Using Generalized Linear Model Atmosphere doi: 10.3390/atmos9080291 Authors: Jinmyeong Jeong Seung-Jae Lee A statistical post-processing method was developed to increase the accuracy of numerical weather prediction (NWP) and simulation by matching the daily distribution of predicted temperatures and wind speeds using the generalized linear model (GLM) and parameter correction, considering an increase in model bias when the range of the prediction time lengthens. The Land Atmosphere Modeling Package Weather Research and Forecasting model, which provides 12-day agrometeorological predictions for East Asia, was employed from May 2017 to April 2018. Training periods occurred one month prior to and after the test period (12 days). A probabilistic consideration accounts for the relatively short training period. Based on the total and monthly root mean square error values for each test site, the results show an improvement in the NWP accuracy after bias correction. The spatial distributions in July and January were compared in detail. It was also shown that the physical consistency between temperature and wind speed was retained in the correction procedure, and that the GLM exhibited better performance than the quantile matching method based on monthly Pearson correlation comparison. The characteristics of coastal and mountainous sites are different from inland automatic weather stations, indicating that supplements to cover these distinctive topographic locations are necessary.

Description: Energies, Vol. 11, Pages 1961: A Novel High Efficiency Quasi-Resonant Converter Energies doi: 10.3390/en11081961 Authors: Tianyu Zhu Jianze Wang Yanchao Ji Yiqi Liu In this paper, a new constant-frequency quasi-resonant converter is proposed. Compared with the traditional LLC converter, the proposed converter can effectively reduce the range of the operating frequency. The output voltage is changed to adjust the reactance of the resonant cavity. The proposed converter has a better loss factor. To verify the theoretical analysis and soft-switching condition, a 250 W, 100 V output prototype was built and compared with the full-bridge LLC converter. Analysis and experimental results verify that a smaller operating frequency range and volume of the transformers, a soft-switching condition, and a higher overall efficiency are achieved with the proposed converter.

Description: Energies, Vol. 11, Pages 1957: Dual-Temperature Evaluation of a High-Temperature Insulation System for Liquid-Immersed Transformer Energies doi: 10.3390/en11081957 Authors: Xiaojing Zhang Lu Ren Haichuan Yu Yang Xu Qingquan Lei Xin Li Baojia Han A high-temperature oil–paper insulation system offers an opportunity to improve the overloading capability of distribution transformers facing seasonal load variation. A high-temperature electrical insulation system (EIS) was chosen due to thermal calculation based on a typical loading curve on the China Southern Power Grid. In order to evaluate candidate high-temperature insulation systems, Nomex® T910 (aramid-enhanced cellulose) immersed in FR3 (natural ester) was investigated by a dual-temperature thermal aging test compared with a conventional insulation system, Kraft paper impregnated with mineral oil. Throughout the thermal aging test, mechanical, chemical, and dielectric parameters of both paper and insulating oil were investigated in each aging cycle. The thermal aging results determined that the thermal class of the FR3-T910 insulation system meets the request of overloading transformer needs.

Description: Energies, Vol. 11, Pages 1954: Experimental Study of Flow-Induced Whistling in Pipe Systems Including a Corrugated Section Energies doi: 10.3390/en11081954 Authors: Hee-Chang LIM Faran RAZI When air flows through pipe systems that include a corrugated segment, a whistling tone is generated and increases in intensity with increasing flow velocity. This whistling sound is related to the particular geometry of corrugated pipes, which is in the form of alternating cavities. This whistling is an environmental noise problem as well as a possible structural danger because of the resulting induced vibration. This paper studies the whistling behavior of various pipe systems with a combination of smooth and corrugated pipes through a series of experiments. The considered pipe systems consist of two smooth pipes attached at the upstream and downstream ends of a corrugated segment. Experiments with smooth and corrugated pipes, which had inner diameters of 15.25 and 16.5 mm, respectively, and various lengths, were performed for flow velocities of up to approximately 30 m/s. The minimum and maximum Strouhal numbers (St) obtained during our experiments were 0.25 and 0.38, respectively. For all pipe configurations investigated in this study, the lowest Mach number at which whistling was observed was 0.017, and the maximum was 0.093. The lowest frequency at which whistling was detected in our experiments was 650 Hz, and the highest was 3080 Hz. The results presented in the form of different variables and dimensionless parameters, including the frequency, Mach number, Strouhal number, and Helmholtz number. The average mode gap and number of excited acoustic modes were also taken into account for all considered configurations. The pipe systems with longer corrugated segments had broader whistling ranges than did configurations with shorter segments, indicating that the number of cavities inside the corrugated pipe has a direct effect on whistling. Increasing the smooth pipe length (either upstream or downstream) resulted in a decrease in the average mode gap between successive modes. The number of excited acoustic modes was primarily related to the corrugated segment length, but the smooth pipe length also had a pronounced effect on the excited modes for a constant corrugation length. The highest number of excited modes (13) was seen in the case of corrugated length 450 mm and smooth pipe length (either upstream or downstream) 400 mm while the lowest number of excited modes (1) was observed for corrugated length 250 mm and smooth pipe length (downstream) 300 mm and 400 mm.

Description: Energies, Vol. 11, Pages 1950: Investigation of Injection Strategy of Branched-Preformed Particle Gel/Polymer/Surfactant for Enhanced Oil Recovery after Polymer Flooding in Heterogeneous Reservoirs Energies doi: 10.3390/en11081950 Authors: Hong He Jingyu Fu Baofeng Hou Fuqing Yuan Lanlei Guo Zongyang Li Qing You The heterogeneous phase combination flooding (HPCF) system which is composed of a branched-preformed particle gel (B-PPG), polymer, and surfactant has been proposed to enhance oil recovery after polymer flooding in heterogeneous reservoirs by mobility control and reducing oil–water interfacial tension. However, the high cost of chemicals can make this process economically challenging in an era of low oil prices. Thus, in an era of low oil prices, it is becoming even more essential to optimize the heterogeneous phase combination flooding design. In order to optimize the HPCF process, the injection strategy has been designed such that the incremental oil recovery can be maximized using the corresponding combination of the B-PPG, polymer, and surfactant, thereby ensuring a more economically-viable recovery process. Different HPCF injection strategies including simultaneous injection and alternation injection were investigated by conducting parallel sand pack flooding experiments and large-scale plate sand pack flooding experiments. Results show that based on the flow rate ratio, the pressure rising area and the incremental oil recovery, no matter whether the injection strategy is simultaneous injection or alternation injection of HPCF, the HPCF can significantly block high permeability zone, increase the sweep efficiency and oil displacement efficiency, and effectively improve oil recovery. Compared with the simultaneous injection mode, the alternation injection of HPCF can show better sweep efficiency and oil displacement efficiency. Moreover, when the slug of HPCF and polymer/surfactant with the equivalent economical cost is injected by alternation injection mode, as the alternating cycle increases, the incremental oil recovery increases. The remaining oil distribution at different flooding stages investigated by conducting large-scale plate sand pack flooding experiments shows that alternation injection of HPCF can recover more remaining oil in the low permeability zone than simultaneous injection. Hence, these findings could provide the guidance for developing the injection strategy of HPCF to further enhance oil recovery after polymer flooding in heterogeneous reservoirs in the era of low oil prices.

Description: Entropy, Vol. 20, Pages 560: Ensemble Estimation of Information Divergence † Entropy doi: 10.3390/e20080560 Authors: Kevin R. Moon Kumar Sricharan Kristjan Greenewald Alfred O. Hero Recent work has focused on the problem of nonparametric estimation of information divergence functionals between two continuous random variables. Many existing approaches require either restrictive assumptions about the density support set or difficult calculations at the support set boundary which must be known a priori. The mean squared error (MSE) convergence rate of a leave-one-out kernel density plug-in divergence functional estimator for general bounded density support sets is derived where knowledge of the support boundary, and therefore, the boundary correction is not required. The theory of optimally weighted ensemble estimation is generalized to derive a divergence estimator that achieves the parametric rate when the densities are sufficiently smooth. Guidelines for the tuning parameter selection and the asymptotic distribution of this estimator are provided. Based on the theory, an empirical estimator of Rényi-α divergence is proposed that greatly outperforms the standard kernel density plug-in estimator in terms of mean squared error, especially in high dimensions. The estimator is shown to be robust to the choice of tuning parameters. We show extensive simulation results that verify the theoretical results of our paper. Finally, we apply the proposed estimator to estimate the bounds on the Bayes error rate of a cell classification problem.

Description: Entropy, Vol. 20, Pages 556: Multivariate Multiscale Complexity Analysis of Self-Reproducing Chaotic Systems Entropy doi: 10.3390/e20080556 Authors: Shaobo He Chunbiao Li Kehui Sun Sajad Jafari Designing a chaotic system with infinitely many attractors is a hot topic. In this paper, multiscale multivariate permutation entropy (MMPE) and multiscale multivariate Lempel–Ziv complexity (MMLZC) are employed to analyze the complexity of those self-reproducing chaotic systems with one-directional and two-directional infinitely many chaotic attractors. The analysis results show that complexity of this class of chaotic systems is determined by the initial conditions. Meanwhile, the values of MMPE are independent of the scale factor, which is different from the algorithm of MMLZC. The analysis proposed here is helpful as a reference for the application of the self-reproducing systems.

Description: IJERPH, Vol. 15, Pages 1592: Full-Scale Experimental Study of Groundwater Softening in a Circulating Pellet Fluidized Reactor International Journal of Environmental Research and Public Health doi: 10.3390/ijerph15081592 Authors: Ruizhu Hu Tinglin Huang Aofan Zhi Zhangcheng Tang The softening effect of a new type of circulating pellet fluidized bed (CPFB) reactor on groundwater was studied through a full-scale experiment. The operation of the CPFB reactor in the second water plant in Chang’an District in Xi’an China was monitored for one year, and the results were compared with those for the Amsterdam reactor in The Netherlands. The removal efficiency of Ca2+ in the CPFB reactor reached 90%; the removal rate of total hardness was higher than 60%; effluent pH was 9.5–9.8; the turbidity of the effluent and the turbidity after boiling were lower than 1.0 NTU; the unit cost was less than €0.064 per m3; and the softened effluent was stable. The pellets in the CPFB reactor were circulated, providing higher crystallization efficiency. The diameter of the discharged pellets reached between 3–5 mm, and the fluidized area height of the CPFB reactor was 4 m. The performance parameters of the CFPB reactor were optimized.

Description: IJERPH, Vol. 15, Pages 1596: Predicting Infectious Disease Using Deep Learning and Big Data International Journal of Environmental Research and Public Health doi: 10.3390/ijerph15081596 Authors: Sangwon Chae Sungjun Kwon Donghyun Lee Infectious disease occurs when a person is infected by a pathogen from another person or an animal. It is a problem that causes harm at both individual and macro scales. The Korea Center for Disease Control (KCDC) operates a surveillance system to minimize infectious disease contagions. However, in this system, it is difficult to immediately act against infectious disease because of missing and delayed reports. Moreover, infectious disease trends are not known, which means prediction is not easy. This study predicts infectious diseases by optimizing the parameters of deep learning algorithms while considering big data including social media data. The performance of the deep neural network (DNN) and long-short term memory (LSTM) learning models were compared with the autoregressive integrated moving average (ARIMA) when predicting three infectious diseases one week into the future. The results show that the DNN and LSTM models perform better than ARIMA. When predicting chickenpox, the top-10 DNN and LSTM models improved average performance by 24% and 19%, respectively. The DNN model performed stably and the LSTM model was more accurate when infectious disease was spreading. We believe that this study’s models can help eliminate reporting delays in existing surveillance systems and, therefore, minimize costs to society.

Description: Materials, Vol. 11, Pages 1301: Spiral Bevel Gears Face Roughness Prediction Produced by CNC End Milling Centers Materials doi: 10.3390/ma11081301 Authors: Álvaro Álvarez Amaia Calleja Mikel Arizmendi Haizea González Luis Norberto Lopez de Lacalle The emergence of multitasking machines in the machine tool sector presents new opportunities for the machining of large size gears and short production series in these machines. However, the possibility of using standard tools in conventional machines for gears machining represents a technological challenge from the point of view of workpiece quality. Machining conditions in order to achieve both dimensional and surface quality requirements need to be determined. With these considerations in mind, computer numerical control (CNC) methods to provide useful tools for gear processing are studied. Thus, a model for the prediction of surface roughness obtained on the teeth surface of a machined spiral bevel gear in a multiprocess machine is presented. Machining strategies and optimal machining parameters were studied, and the roughness model is validated for 3 + 2 axes and 5 continuous axes machining strategies.

Description: Materials, Vol. 11, Pages 1302: Enhanced Cycling Stability of LiCuxMn1.95−xSi0.05O4 Cathode Material Obtained by Solid-State Method Materials doi: 10.3390/ma11081302 Authors: Hongyuan Zhao Fang Li Xiuzhi Bai Tingting Wu Zhankui Wang Yongfeng Li Jianxiu Su The LiCuxMn1.95−xSi0.05O4 (x = 0, 0.02, 0.05, 0.08) samples have been obtained by a simple solid-state method. XRD and SEM characterization results indicate that the Cu-Si co-doped spinels retain the inherent structure of LiMn2O4 and possess uniform particle size distribution. Electrochemical tests show that the optimal Cu-doping amount produces an obvious improvement effect on the cycling stability of LiMn1.95Si0.05O4. When cycled at 0.5 C, the optimal LiCu0.05Mn1.90Si0.05O4 sample exhibits an initial capacity of 127.3 mAh g−1 with excellent retention of 95.7% after 200 cycles. Moreover, when the cycling rate climbs to 10 C, the LiCu0.05Mn1.90Si0.05O4 sample exhibits 82.3 mAh g−1 with satisfactory cycling performance. In particular, when cycled at 55 °C, this co-doped sample can show an outstanding retention of 94.0% after 100 cycles, whiles the LiMn1.95Si0.05O4 only exhibits low retention of 79.1%. Such impressive performance shows that the addition of copper ions in the Si-doped spinel effectively remedy the shortcomings of the single Si-doping strategy and the Cu-Si co-doped spinel can show excellent cycling stability.

Description: Forests, Vol. 9, Pages 459: Pedunculate and Sessile Mixed Oak Forest Regeneration Process in Lithuania Forests doi: 10.3390/f9080459 Authors: Girmantė Jurkšienė Virginijus Baliuckas Pedunculate and sessile oak species are sympatric. These oaks hybridize with one another, and this process influences the development of undergrowth. The purpose of this study was to determine how different oak species influence the forest regeneration process. For this purpose, the forest was divided into eight transects of 300 m and 100 m widths, distinguished into temporary plots of 10 m in diameter covering the whole territory of the forest. The distribution of oak undergrowth was calculated by four oak height groups, determining the composition of the first storey, covering of underbrush and herbaceous plant, and forest site. We determined that the spread of oak differed depending on the first storey tree species and underbrush. Grass cover was the biggest influence on the sessile oak. The impurity of sessile oak in oak stands had a positive impact on the development of undergrowth, since the entire undergrowth develops faster than separate components of the undergrowth.

Description: Forests, Vol. 9, Pages 455: Measuring Eco-Efficiency of State-Owned Forestry Enterprises in Northeast China Forests doi: 10.3390/f9080455 Authors: Youliang Ning Zhen Liu Zekui Ning Han Zhang State-owned forestry enterprises (SOFEs) play an important role in the forestry economy in China. Understanding the eco-efficiency of their production is beneficial for the development of sustainable forestry and for achieving Goal 8 of the United Nations’ Sustainable Development Goals (SDGs): Decent Work and Economic Growth. This paper assesses SOFEs’ overall eco-efficiency by analyzing various undesirable outputs using the Slacks-Based Measure of efficiency in Data Envelopment Analysis (SBM-DEA) model. Using basic data from 87 SOFEs in Northeast China from 2003 to 2016, this paper evaluated the eco-efficiency development level and spatial patterns of that region. The results show that SOFEs’ low eco-efficiency was caused by low pure-technical efficiency. Regional differences in eco-efficiency were very significant and became larger, but a market-oriented reform might help to improve such efficiency. The eco-efficiency of SOFEs was in decline from 2003 to 2016 due to the implementation of the Natural Forest Protection Project (NFPP). However, due to a relative lack of production factor inputs, most SOFEs’ scale returns are now increasing. In the future, efforts should be made to promote market-oriented reforms and take the path of large-scale development.

Description: Minerals, Vol. 8, Pages 323: (10.4) Face of Ordered and Disordered Dolomite, MgCa(CO3)2: A Computational Study to Reveal the Growth Mechanism Minerals doi: 10.3390/min8080323 Authors: Marco Bruno Erica Bittarello In this study, the stability of the (10.4) face of dolomite was systematically investigated. The surface energies at 0 K of the different (10.4) surfaces resulting from the cut of both ordered and disordered bulk structures were determined and compared, to establish how different atomic configurations (surface terminations) can affect the stability of the investigated face. To study the thermodynamic behavior of a surface, a 2D periodic slab model and the ab initio CRYSTAL code were adopted. The surface energies of the (10.4) faces of calcite and magnesite were also calculated in order to compare them with those of the different terminations of the (10.4) face of dolomite. Our calculations showed that the bulk of the dolomite crystal must have an ordered structure to reach the minimum of the energy, whereas the (10.4) surface is more stable when its structure is disordered. A growth model of the (10.4) face has been proposed: the peculiarity of this model consists in the existence of some disordered layers forming at the interface crystal/solution, which arrange in an ordered structure once covered by others disordered layers resulting by the spiral steps propagation.

Description: Materials, Vol. 11, Pages 1297: A Straightforward Substitution Strategy to Tune BODIPY Dyes Spanning the Near-Infrared Region via Suzuki–Miyaura Cross-Coupling Materials doi: 10.3390/ma11081297 Authors: Guanglei Li Yu Otsuka Takuya Matsumiya Toshiyuki Suzuki Jianye Li Masashi Takahashi Koji Yamada In this study, a series of new red and near-infrared (NIR) dyes derived from 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) were developed by introducing thiophene and its derivatives to the 3- and 5- positions of the dichloroBODIPY core. For the first time, cyclictriol boronates and N-methyliminodiacetic acid (MIDA) boronate were used as organoboron species to couple with 3,5-dichloroBODIPY via the one-step Suzuki–Miyaura cross-coupling. Six kinds of thieno-expended BODIPY dyes were synthesized in acceptable yields ranging from 31% to 79%. All six dyes showed different absorption and emission wavelengths spanning a wide range (c.a. 600–850 nm) in the red and NIR regions with relatively high quantum yields (19–85%). Cellular imaging of 8-(2,6-dimethylphenyl)-re3,5-di(2-thienyl)-BODIPY (dye 1) was conducted using bovine cumulus cells, and the fluorescence microscopy images indicated that the chromophore efficiently accumulated and was exclusively localized in the cytoplasm, suggesting it could be utilized as a subcellular probe. All six dyes were characterized using 1H-NMR and mass spectrometry.

Description: Minerals, Vol. 8, Pages 322: Particle Size Distribution Effects on the Strength Characteristic of Cemented Paste Backfill Minerals doi: 10.3390/min8080322 Authors: Jiangyu Wu Meimei Feng Zhanqing Chen Xianbiao Mao Guansheng Han Yiming Wang It is of great significance, for economic, environmental and security reasons, to investigate the strength characteristic of underground cemented paste backfill (CPB). Consequently, an ultrasonic test, uniaxial and triaxial compression experiment, and acoustic emission (AE) monitoring were carried out on CPB, for which the particles satisfied Talbot gradation. The homogeneity of CPB specimens was evaluated by ultrasonic pulse velocity (UPV). The stress–strain behavior and AE characteristic of CPB specimens under different Talbot indices and confining pressures were investigated. The effects of the particle size distribution and the confining pressure on the peak strength of CPB were analyzed. The strength parameter model of CPB under the coupled influence of the particle size distribution and the confining pressure was constructed based on the Mohr–Coulomb strength criterion. The results show that the peak strength of CPB is positively linear with confining pressure, however, the relationship between its strength parameters and the Talbot index can be characterized by a quadratic polynomial function. This suggests that there is an optimal gradation of particles reflected in the maximum strength of CPB.

Description: Minerals, Vol. 8, Pages 319: Fault Zone Evolution and Development of a Structural and Hydrological Barrier: The Quartz Breccia in the Kiggavik Area (Nunavut, Canada) and Its Control on Uranium Mineralization Minerals doi: 10.3390/min8080319 Authors: Alexis Grare Olivier Lacombe Julien Mercadier Antonio Benedicto Marie Guilcher Anna Trave Patrick Ledru John Robbins In the Kiggavik area (Nunavut, Canada), major fault zones along, or close to, where uranium deposits are found are often associated with occurrence of thick quartz breccia (QB) bodies. These bodies formed in an early stage (~1750 Ma) of the long-lasting tectonic history of the Archean basement, and of the Proterozoic Thelon basin. The main characteristics of the QB are addressed in this study; through field work, macro and microscopic observations, cathodoluminescence microscopy, trace elements, and oxygen isotopic signatures of the quartz forming the QB. Faults formed earlier during syn- to post-orogenic rifting (1850–1750 Ma) were subsequently reactivated, and underwent cycles of cataclasis, pervasive silicification, hydraulic brecciation, and quartz recrystallization. This was synchronous with the circulation of meteoric fluids mixing with Si-rich magmatic-derived fluids at depth, and were coeval with the emplacement of the Kivalliq igneous suite at 1750 Ma. These processes led to the emplacement of up to 30 m thick QB, which behaved as a mechanically strong, transverse hydraulic barrier that localized later fracturing, and compartmentalized/channelized vertical flow of uranium-bearing fluids after the deposition of the Thelon Basin (post 1750 Ma). The development and locations of QB control the location of uranium mineralization in the Kiggavik area.

Description: IJERPH, Vol. 15, Pages 1597: Assessing Heat-Related Mortality Risks among Rural Populations: A Systematic Review and Meta-Analysis of Epidemiological Evidence International Journal of Environmental Research and Public Health doi: 10.3390/ijerph15081597 Authors: Emmanuel A. Odame Ying Li Shimin Zheng Ambarish Vaidyanathan Ken Silver Most epidemiological studies of high temperature effects on mortality have focused on urban settings, while heat-related health risks in rural areas remain underexplored. To date there has been no meta-analysis of epidemiologic literature concerning heat-related mortality in rural settings. This study aims to systematically review the current literature for assessing heat-related mortality risk among rural populations. We conducted a comprehensive literature search using PubMed, Web of Science, and Google Scholar to identify articles published up to April 2018. Key selection criteria included study location, health endpoints, and study design. Fourteen studies conducted in rural areas in seven countries on four continents met the selection criteria, and eleven were included in the meta-analysis. Using the random effects model, the pooled estimates of relative risks (RRs) for all-cause and cardiovascular mortality were 1.030 (95% CI: 1.013, 1.048) and 1.111 (95% CI: 1.045, 1.181) per 1 °C increase in daily mean temperature, respectively. We found excess risks in rural settings not to be smaller than risks in urban settings. Our results suggest that rural populations, like urban populations, are also vulnerable to heat-related mortality. Further evaluation of heat-related mortality among rural populations is warranted to develop public health interventions in rural communities.

Description: IJERPH, Vol. 15, Pages 1595: Does SuperPark Make Children Less Sedentary? How Visiting a Commercial Indoor Activity Park Affects 7 to 12 Years Old Children’s Daily Sitting and Physical Activity Time International Journal of Environmental Research and Public Health doi: 10.3390/ijerph15081595 Authors: Arto J. Pesola Martti Melin Anssi Vanhala Ying Gao Taija Finni Commercial indoor activity parks provide children with a variety of entertaining physical activities. This study examined whether visiting SuperPark affects total daily sitting and physical activity time. The participants (8 girls and 7 boys, aged 10.3 ± 1.9 years, height 144.5 ± 11.8 cm, body mass index (BMI) 19.3 ± 3.0 kg/m2) wore a thigh-worn accelerometer during a normal week and were provided free tickets to visit SuperPark on at least one day. On average, the children spent 3.3 ± 1.2 h in SuperPark. During the visits the children had 0.9 h less sitting (0.7 ± 0.3 h, p = 0.000) and 0.9 h more moderate-to-vigorous physical activity (MVPA; 1.4 ± 0.6 h, p = 0.002) as compared to the reference periods on days without a SuperPark visit (1.6 ± 0.3 h sitting and 0.5 ± 0.4 h MVPA). During the days when visiting SuperPark, sitting time decreased 1.0 h (5.8 ± 0.9 h, p = 0.008) and MVPA increased 0.8 h (3.0 ± 1.0 h, p = 0.017) as compared to the reference days (6.8 ± 1.1 h sitting and 2.2 ± 0.8 h MVPA). The effects were more pronounced during weekdays than weekends. The children spent more than three hours in SuperPark on one visit, of which almost a half was MVPA. During the whole day, one hour of sitting was replaced with MVPA, suggesting that visiting SuperPark has the potential to improve health. Whether children continue visiting SuperPark and gain health benefits merits investigation.

Description: IJGI, Vol. 7, Pages 300: A Comparative Study of Three Non-Geostatistical Methods for Optimising Digital Elevation Model Interpolation ISPRS International Journal of Geo-Information doi: 10.3390/ijgi7080300 Authors: Serajis Salekin Jack H. Burgess Justin Morgenroth Euan G. Mason Dean F. Meason It is common to generate digital elevation models (DEMs) from aerial laser scanning (ALS) data. However, cost and lack of knowledge may preclude its use. In contrast, global navigation satellite systems (GNSS) are seldom used to collect and generate DEMs. These receivers have the potential to be considered as data sources for DEM interpolation, as they can be inexpensive, easy to use, and mobile. The data interpolation method and spatial resolution from this method needs to be optimised to create accurate DEMs. Moreover, the density of GNSS data is likely to affect DEM accuracy. This study investigates three different deterministic approaches, in combination with spatial resolution and data thinning, to determine their combined effects on DEM accuracy. Digital elevation models were interpolated, with resolutions ranging from 0.5 m to 10 m using natural neighbour (NaN), topo to raster (ANUDEM), and inverse distance weighted (IDW) methods. The GNSS data were thinned by 25% (0.389 points m−2), 50% (0.259 points m−2), and 75% (0.129 points m−2) and resulting DEMs were contrast against a DEM interpolated from unthinned data (0.519 points m−2). Digital elevation model accuracy was measured by root mean square error (RMSE) and mean absolute error (MAE). It was found that the highest resolution, 0.5 m, produced the lowest errors in resulting DEMs (RMSE = 0.428 m, MAE = 0.274 m). The ANUDEM method yielded the greatest DEM accuracy from a quantitative perspective (RMSE = 0.305 m and MAE = 0.197 m); however, NaN produced a more visually appealing surface. In all the assessments, IDW showed the lowest accuracy. Thinning the input data by 25% and even 50% had relatively little impact on DEM quality; however, accuracy decreased markedly at 75% thinning (0.129 points m−2). This study showed that, in a time where ALS is commonly used to generate DEMs, GNSS-surveyed data can be used to create accurate DEMs. This study confirmed the need for optimization to choose the appropriate interpolation method and spatial resolution in order to produce a reliable DEM.

Description: IJERPH, Vol. 15, Pages 1593: Modeling Land Use Changes and their Impacts on Non-Point Source Pollution in a Southeast China Coastal Watershed International Journal of Environmental Research and Public Health doi: 10.3390/ijerph15081593 Authors: Xin Zhang Lin Zhou Yuqi Liu Changes in landscape patterns in a river basin play a crucial role in the change on load of non-point source pollution. The spatial distribution of various land use types affects the transmission of non-point source pollutants on the basis of source-sink theory in landscape ecology. Jiulong River basin in southeast of China was selected as the study area in this paper. Aiming to analyze the correlation between changing landscape patterns and load of non-point source pollution in this area, traditional landscape metrics and the improved location-weighted landscape contrast index based on the minimum hydrological response unit (HRULCI) were applied in this study, in combination with remote sensing and geographic information system (GIS) technique. The results of the landscape metrics showed the enhanced fragmentation extent and the decreasing polymerization degree of the overall landscape in the watershed. High values of HRULCI were concentrated in cultivated land, while low HRULCI values mostly appeared in forestland, indicating that cultivated land substantially enhanced non-point source pollution, while forestland inhibited the pollution process.

Description: by Keira Sztukowski, Kaila Nip, Paige N. Ostwald, Matheus F. Sathler, Julianna L. Sun, Jiayi Shou, Emily T. Jorgensen, Travis E. Brown, John H. Elder, Craig Miller, Franz Hofmann, Sue VandeWoude, Seonil Kim Over half of individuals infected with human immunodeficiency virus (HIV) suffer from HIV-associated neurocognitive disorders (HANDs), yet the molecular mechanisms leading to neuronal dysfunction are poorly understood. Feline immunodeficiency virus (FIV) naturally infects cats and shares its structure, cell tropism, and pathology with HIV, including wide-ranging neurological deficits. We employ FIV as a model to elucidate the molecular pathways underlying HIV-induced neuronal dysfunction, in particular, synaptic alteration. Among HIV-induced neuron-damaging products, HIV envelope glycoprotein gp120 triggers elevation of intracellular Ca 2+ activity in neurons, stimulating various pathways to damage synaptic functions. We quantify neuronal Ca 2+ activity using intracellular Ca 2+ imaging in cultured hippocampal neurons and confirm that FIV envelope glycoprotein gp95 also elevates neuronal Ca 2+ activity. In addition, we reveal that gp95 interacts with the chemokine receptor, CXCR4, and facilitates the release of intracellular Ca 2+ by the activation of the endoplasmic reticulum (ER)-associated Ca 2+ channels, inositol triphosphate receptors (IP3Rs), and synaptic NMDA receptors (NMDARs), similar to HIV gp120. This suggests that HIV gp120 and FIV gp95 share a core pathological process in neurons. Significantly, gp95’s stimulation of NMDARs activates cGMP-dependent protein kinase II (cGKII) through the activation of the neuronal nitric oxide synthase (nNOS)-cGMP pathway, which increases Ca 2+ release from the ER and promotes surface expression of AMPA receptors, leading to an increase in synaptic activity. Moreover, we culture feline hippocampal neurons and confirm that gp95-induced neuronal Ca 2+ overactivation is mediated by CXCR4 and cGKII. Finally, cGKII activation is also required for HIV gp120-induced Ca 2+ hyperactivation. These results thus provide a novel neurobiological mechanism of cGKII-mediated synaptic hyperexcitation in HAND.

Description: by Weilong Chen, Yuanyuan Qin, Dong Wang, Lei Zhou, Yin Liu, Sheng Chen, Liang Yin, Yaoxing Xiao, Xiao-Hong Yao, Xiaoli Yang, Wei Ma, Weifeng Chen, Xueyan He, Lixing Zhang, Qifeng Yang, Xiuwu Bian, Zhi-ming Shao, Suling Liu Chemotherapeutic resistance in triple-negative breast cancer (TNBC) has brought great challenges to the improvement of patient survival. The mechanisms of taxane chemoresistance in TNBC have not been well investigated. Our results illustrated C-C motif chemokine ligand 20 (CCL20) was significantly elevated during taxane-containing chemotherapy in breast cancer patients with nonpathologic complete response. Furthermore, CCL20 promoted the self-renewal and maintenance of breast cancer stem cells (BCSCs) or breast cancer stem-like cells through protein kinase Cζ (PKCζ) or p38 mitogen-activated protein kinase (MAPK)-mediated activation of p65 nuclear factor kappa B (NF-κB) pathway, significantly increasing the frequency and taxane resistance of BCSCs. Moreover, CCL20-promoted NF-κB activation increased ATP-binding cassette subfamily B member 1 (ABCB1)/multidrug resistance 1 (MDR1) expression, leading to the extracellular efflux of taxane. These results suggested that chemotherapy-induced CCL20 mediated chemoresistance via up-regulating ABCB1. In addition, NF-κB activation increased CCL20 expression, forming a positive feedback loop between NF-κB and CCL20 pathways, which provides sustained impetus for chemoresistance in breast cancer cells. Our results suggest that CCL20 can be a novel predictive marker for taxane response, and the blockade of CCL20 or its downstream pathway might reverse the taxane resistance in breast cancer patients.

Description: by Luigi Acerbi, Kalpana Dokka, Dora E. Angelaki, Wei Ji Ma The precision of multisensory perception improves when cues arising from the same cause are integrated, such as visual and vestibular heading cues for an observer moving through a stationary environment. In order to determine how the cues should be processed, the brain must infer the causal relationship underlying the multisensory cues. In heading perception, however, it is unclear whether observers follow the Bayesian strategy, a simpler non-Bayesian heuristic, or even perform causal inference at all. We developed an efficient and robust computational framework to perform Bayesian model comparison of causal inference strategies, which incorporates a number of alternative assumptions about the observers. With this framework, we investigated whether human observers’ performance in an explicit cause attribution and an implicit heading discrimination task can be modeled as a causal inference process. In the explicit causal inference task, all subjects accounted for cue disparity when reporting judgments of common cause, although not necessarily all in a Bayesian fashion. By contrast, but in agreement with previous findings, data from the heading discrimination task only could not rule out that several of the same observers were adopting a forced-fusion strategy, whereby cues are integrated regardless of disparity. Only when we combined evidence from both tasks we were able to rule out forced-fusion in the heading discrimination task. Crucially, findings were robust across a number of variants of models and analyses. Our results demonstrate that our proposed computational framework allows researchers to ask complex questions within a rigorous Bayesian framework that accounts for parameter and model uncertainty.

Description: by Simon Heilbronner, Ian R. Monk, Jeremy R. Brozyna, David E. Heinrichs, Eric P. Skaar, Andreas Peschel, Timothy J. Foster

Description: by Ida Friis, Ilia A. Solov’yov The non-homologous end joining of a DNA double strand break is initiated by the MRE11-NBS1-RAD50 complex whose subunits are the first three proteins to arrive to the breakage site thereby making the recruitment time of MRE11, NBS1 and RAD50 essential for cell survival. In the present investigation, the nature of MRE11 and NBS1 transportation from the cytoplasm to the nucleus, hosting the damaged DNA strand, is hypothesized to be a passive diffusive process. The feasibility of such a mechanism is addressed through theoretical and computational approaches which permit establishing the characteristic recruitment time of MRE11 and NBS1 by the nucleus. A computational model of a cell is constructed from a set of biological parameters and the kinetic Monte Carlo algorithm is used to simulate the diffusing MRE11 and NBS1 particles as a random walk process. To accurately describe the experimented data, it is discovered that MRE11 and NBS1 should start diffusion from significantly different starting positions which suggests that diffusion might not be the only transport mechanism of repair protein recruitment to the DNA break.

Description: by Lin Yang, Xiaoyan Kong, Shuli Yang, Xinxing Dong, Jianfa Yang, Xiao Gou, Hao Zhang The Tibetan horse is a species endemic to the Tibetan plateau, with considerable economic value in the region. However, we currently have little genetic evidence to verify whether the breed originated in Tibet or if it entered the area via an ancient migratory route. In the present study, we analyzed the hypervariable segment I sequences of mitochondrial DNA (mtDNA) in 2,050 horses, including 290 individuals from five Tibetan populations and 1,760 from other areas across Asia. Network analysis revealed multiple maternal lineages in the Tibetan horse. Component analysis of sub-lineage F3 indicated that it decreased in frequency from east to west, a trend reflected both southward and northward from Inner Mongolia. Analysis of population genetics showed that the Deqen horse of eastern Tibet was more closely related to the Ningqiang horse of northern China than to other Tibetan horses or the Yunnan horse. These results indicated that the Tibetan horse migrated first from Central Asia to Mongolia, moved south to eastern Tibet (near Deqen), then finally westward to other regions of Tibet. We also identified a novel lineage K that mainly comprises Tibetan and Yunnan horses, suggesting autochthonous domesticated origin for some Tibetan horse breeds from local wild horses. In conclusion, our study demonstrated that modern Tibetan horse breeds originated from the introgression of local wild horses with exotic domesticated populations outside China.

Description: by Marya Plotkin, Dunstan Bishanga, Hussein Kidanto, Mary Carol Jennings, Jim Ricca, Amasha Mwanamsangu, Gaudiosa Tibaijuka, Ruth Lemwayi, Benny Ngereza, Mary Drake, Jeremie Zougrana, Neena Khadka, James A. Litch, Barbara Rawlins Background Globally, an estimated 2.7 million babies die in the neonatal period annually, and of these, about 0.7 million die from intrapartum-related events. In Tanzania 51,000 newborn deaths and 43,000 stillbirths occur every year. Approximately two-thirds of these deaths could be potentially prevented with improvements in intrapartum and neonatal care. Routine measurement of fetal intrapartum deaths and newborn deaths that occur in health facilities can help to evaluate efforts to improve the quality of intrapartum care to save lives. However, few examples exist of indicators on perinatal mortality in the facility setting that are readily available through health management information systems (HMIS). Methods From November 2016 to April 2017, health providers at 10 government health facilities in Kagera region, Tanzania, underwent refresher training on perinatal death classification and training on the use of handheld Doppler devices to assess fetal heart rate upon admission to maternity services. Doppler devices were provided to maternity services at the study facilities. We assessed the validity of an indicator to measure facility-based pre-discharge perinatal mortality by comparing perinatal outcomes extracted from the HMIS maternity registers to a gold standard perinatal death audit. Results Sensitivity and specificity of the HMIS neonatal outcomes to predict gold standard audit outcomes were both over 98% based on analysis of 128 HMIS–gold standard audit pairs. After this validation, we calculated facility perinatal mortality indicator from HMIS data using fresh stillbirths and pre-discharge newborn death as the numerator and women admitted in labor with positive fetal heart tones as the denominator. Further emphasizing the validity of the indicator, FPM values aligned with expected mortality by facility level, with lowest rates in health centers (range 0.3%– 0.5%), compared to district hospitals (1.5%– 2.9%) and the regional hospital (4.2%). Conclusion This facility perinatal mortality indicator provides an important health outcome measure that facilities can use to monitor levels of perinatal deaths occurring in the facility and evaluate impact of quality of care improvement activities.

Description: by Seung-Heon Shin, Mi-Kyung Ye, Dong-Won Lee, Mi-Hyun Che The essential oil of Chamaecyparis obtusa ( C . obtusa ), which is used in soap, toothpaste, and aromatic agents, has been known to have anti-inflammatory properties. In this study, we investigated the effects of microencapsulated C . obtusa essential oil on airborne fungus-induced dendritic cell (DC) activation and Th immune responses. We stimulated monocyte-derived DCs with Alternaria alternate and lipopolysaccharide (LPS). To determine the anti-inflammatory effects, we pre-treated DCs with various concentrations of microencapsulated C . obtusa essential oil and collected the supernatants to measure interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), and we determined the expression of cell surface molecules. The effects of the essential oil on CD4 + T cells polarization was determine by culturing stimulated DCs and autologous CD4 + T cells. Alternaria enhanced the production of IL-6 and TNF-α from DCs, and pretreating DCs with 0.001, 0.01, and 0.05% of the essential oil significantly inhibited their production. Increased CD80 and CD86 expression by Alternaria was significantly inhibited with 0.05% of the essential oil. Alternaria -induced IL-5, IL-10, and interferon-gamma from CD4 + T cells were significantly inhibited with C . obtusa essential oil in a dose dependent manner. C . obtusa influenced both Alternaria - and LPS-induced Th1 and Th2 polarization of CD4 + T cells. These results suggest a novel pharmacological use for C . obtusa essential oil to treat inflammatory airway diseases.

Description: by P. Susthitha Menon, Fairus Atida Said, Gan Siew Mei, Dilla Duryha Berhanuddin, Akrajas Ali Umar, Sahbudin Shaari, Burhanuddin Yeop Majlis This work investigates the surface plasmon resonance (SPR) response of 50-nm thick nano-laminated gold film using Kretschmann-based biosensing for detection of urea and creatinine in solution of various concentrations (non-enzymatic samples). Comparison was made with the presence of urease and creatininase enzymes in the urea and creatinine solutions (enzymatic samples), respectively. Angular interrogation technique was applied using optical wavelengths of 670 nm and 785 nm. The biosensor detects the presence of urea and creatinine at concentrations ranging from 50–800 mM for urea samples and 10–200 mM for creatinine samples. The purpose of studying the enzymatic sample was mainly to enhance the sensitivity of the sensor towards urea and creatinine in the samples. Upon exposure to 670 nm optical wavelength, the sensitivity of 1.4°/M was detected in non-enzymatic urea samples and 4°/M in non-enzymatic creatinine samples. On the other hand, sensor sensitivity as high as 16.2°/M in urea-urease samples and 10°/M in creatinine-creatininase samples was detected. The enhanced sensitivity possibly attributed to the increase in refractive index of analyte sensing layer due to urea-urease and creatinine-creatininase coupling activity. This work has successfully proved the design and demonstrated a proof-of-concept experiment using a low-cost and easy fabrication of Kretschmann based nano-laminated gold film SPR biosensor for detection of urea and creatinine using urease and creatininase enzymes.

Description: by David A. Eavarone, Linah Al-Alem, Alexey Lugovskoy, Jillian M. Prendergast, Rawan I. Nazer, Jenna N. Stein, Daniel T. Dransfield, Jeff Behrens, Bo R. Rueda The expression of Sialyl-Tn (STn) in tumors is associated with metastatic disease, poor prognosis, and reduced overall survival. STn is expressed on ovarian cancer biomarkers including CA-125 (MUC16) and MUC1, and elevated serum levels of STn in ovarian cancer patients correlate with lower five-year survival rates. In the current study, we humanized novel anti-STn antibodies and demonstrated the retention of nanomolar (nM) target affinity while maintaining STn antigen selectivity. STn antibodies conjugated to Monomethyl Auristatin E (MMAE-ADCs) demonstrated in vitro cytotoxicity specific to STn-expressing ovarian cancer cell lines and tumor growth inhibition in vivo with both ovarian cancer cell line- and patient-derived xenograft models. We further validated the clinical potential of these STn-ADCs through tissue cross-reactivity and cynomolgus monkey toxicity studies. No membrane staining for STn was present in any organs of human or cynomolgus monkey origin, and the toxicity profile was favorable and only revealed MMAE-class associated events with none being attributed to the targeting of STn. The up-regulation of STn in ovarian carcinoma in combination with high affinity and STn-specific selectivity of the mAbs presented herein warrant further investigation for anti-STn antibody-drug conjugates in the clinical setting.

Description: by Laura K. Borkenhagen, Kerry A. Mallinson, Rick W. Tsao, Siaw-Jing Ha, Wei-Honn Lim, Teck-Hock Toh, Benjamin D. Anderson, Jane K. Fieldhouse, Sarah E. Philo, Kuek-Sen Chong, William G. Lindsley, Alejandro Ramirez, James F. Lowe, Kristen K. Coleman, Gregory C. Gray Background The large livestock operations and dense human population of Southeast Asia are considered a hot-spot for emerging viruses. Objectives To determine if the pathogens adenovirus (ADV), coronavirus (CoV), encephalomyocarditis virus (EMCV), enterovirus (EV), influenza A-D (IAV, IBV, ICV, and IDV), porcine circovirus 2 (PCV2), and porcine rotaviruses A and C (RVA and RVC), are aerosolized at the animal-interface, and if humans working in these environments are carrying these viruses in their nasal airways. Study This cross-sectional study took place in Sarawak, Malaysia among 11 pig farms, 2 abattoirs, and 3 animal markets in June and July of 2017. Pig feces, pig oral secretions, bioaerosols, and worker nasal wash samples were collected and analyzed via rPCR and rRT-PCR for respiratory and diarrheal viruses. Results In all, 55 pig fecal, 49 pig oral or water, 45 bioaerosol, and 78 worker nasal wash samples were collected across 16 sites. PCV2 was detected in 21 pig fecal, 43 pig oral or water, 3 bioaerosol, and 4 worker nasal wash samples. In addition, one or more bioaerosol or pig samples were positive for EV, IAV, and RVC, and one or more worker samples were positive for ADV, CoV, IBV, and IDV. Conclusions This study demonstrates that nucleic acids from a number of targeted viruses were present in pig oral secretions and pig fecal samples, and that several viruses were detected in bioaerosol samples or in the nasal passages of humans with occupational exposure to pigs. These results demonstrate the need for future research in strengthening viral surveillance at the human-animal interface, specifically through expanded bioaerosol sampling efforts and a seroepidemiological study of individuals with exposure to pigs in this region for PCV2 infection.

Description: by Vasileios Stavropoulos, Stefanos Mastrotheodoros, Tyrone L. Burleigh, Nicole Papadopoulos, Rapson Gomez Romantic development is a distinctive characteristic of puberty. However, a significant proportion of adolescents present with avoidant romantic attachment (ARA) tendencies, which have significant impact on their general adaptation. ARA variations have been suggested in relation to age, gender, engagement with a romantic partner and Excessive Internet Use (EIU) behaviours. In this longitudinal, two-wave study of a normative sample of 515 Greek adolescents at 16 and 18 years, ARA was assessed with the relevant subscale of the Experiences in Close Relationships-Revised and EIU with the Internet Addiction Test. A three-level hierarchical linear model found ARA tendencies to decrease between 16 and 18 while engagement in a romantic relationship and EIU were associated with lower and higher ARA tendencies respectively. Gender did not differentiate ARA severity either at the age of 16 or its changes over time. Results highlight the need of adopting a longitudinal-contextualized approach and provide implications for prevention and intervention initiatives in relation to the romantic development of adolescents.

Description: by Emma C. Johnson, Luke M. Evans, Matthew C. Keller Inbreeding increases the risk of certain Mendelian disorders in humans but may also reduce fitness through its effects on complex traits and diseases. Such inbreeding depression is thought to occur due to increased homozygosity at causal variants that are recessive with respect to fitness. Until recently it has been difficult to amass large enough sample sizes to investigate the effects of inbreeding depression on complex traits using genome-wide single nucleotide polymorphism (SNP) data in population-based samples. Further, it is difficult to infer causation in analyses that relate degree of inbreeding to complex traits because confounding variables (e.g., education) may influence both the likelihood for parents to outbreed and offspring trait values. The present study used runs of homozygosity in genome-wide SNP data in up to 400,000 individuals in the UK Biobank to estimate the proportion of the autosome that exists in autozygous tracts—stretches of the genome which are identical due to a shared common ancestor. After multiple testing corrections and controlling for possible sociodemographic confounders, we found significant relationships in the predicted direction between estimated autozygosity and three of the 26 traits we investigated: age at first sexual intercourse, fluid intelligence, and forced expiratory volume in 1 second. Our findings corroborate those of several published studies. These results may imply that these traits have been associated with Darwinian fitness over evolutionary time. However, some of the autozygosity-trait relationships were attenuated after controlling for background sociodemographic characteristics, suggesting that alternative explanations for these associations have not been eliminated. Care needs to be taken in the design and interpretation of ROH studies in order to glean reliable information about the genetic architecture and evolutionary history of complex traits.

Description: by David M. Brown, Sarah Jones, Zoe C. T. R. Daniel, Madelaine C. Brearley, Jo E. Lewis, Francis J. P. Ebling, Tim Parr, John M. Brameld Previously, we highlighted induction of an integrated stress response (ISR) gene program in skeletal muscle of pigs treated with a beta-adrenergic agonist. Hence we tested the hypothesis that the ER-stress inhibitor, sodium 4-phenylbutyrate (PBA), would inhibit Clenbuterol-mediated muscle growth and reduce expression of genes that are known indicators of an ISR in mice. Clenbuterol (1mg/kg/day) administered to C57BL6/J mice for 21 days increased body weight ( p

Description: by Charlotte Avet, Chantal Denoyelle, David L’Hôte, Florence Petit, Céline J. Guigon, Joëlle Cohen-Tannoudji, Violaine Simon Reproductive function is under the control of the neurohormone GnRH, which activates a G-protein-coupled receptor (GnRHR) expressed in pituitary gonadotrope cells. GnRHR activates a complex signaling network to regulate synthesis and secretion of the two gonadotropin hormones, luteinizing hormone and follicle-stimulating hormone, both regulating gametogenesis and steroidogenesis in gonads. Recently, in an attempt to identify the mechanisms underlying GnRHR signaling plasticity, we identified the first interacting partner of GnRHR, the proto-oncogene SET. We showed that SET binds to intracellular domains of GnRHR to enhance its coupling to cAMP pathway in αT3-1 gonadotrope cells. Here, we demonstrate that SET protein is rapidly regulated by GnRH, which increases SET phosphorylation state and decreases dose-dependently SET protein level. Our results highlight a post-translational regulation of SET protein involving the proteasome pathway. We determined that SET phosphorylation upon GnRH stimulation is mediated by PKC and that PKC mediates GnRH-induced SET down-regulation. Phosphorylation on serine 9 targets SET for degradation into the proteasome. Furthermore, a non-phosphorylatable SET mutant on serine 9 is resistant to GnRH-induced down-regulation. Altogether, these data suggest that GnRH-induced SET phosphorylation on serine 9 mediates SET protein down-regulation through the proteasome pathway. Noteworthy, SET down-regulation was also observed in response to pulsatile GnRH stimulation in LβT2 gonadotrope cells as well as in vivo in prepubertal female mice supporting its physiological relevance. In conclusion, this study highlights a regulation of SET protein by the neurohormone GnRH and identifies some of the mechanisms involved.

Description: by Wilson Mendoza, Dominick Mendola, Jang Kim, Charles Yarish, Alyssa Velloze, B. Greg Mitchell

Description: by Àngel Oliveras, Aina Baró, Laura Montesinos, Esther Badosa, Emilio Montesinos, Lidia Feliu, Marta Planas A collection of 36 lipopeptides were designed from the cecropin A-melittin hybrid peptide BP100 (H-Lys-Lys-Leu-Phe-Lys-Lys-Ile-Leu-Lys-Tyr-Leu-NH 2 ) previously described with activity against phytopathogenic bacteria. These lipopeptides were synthesized on solid-phase and screened for their antimicrobial activity, toxicity and proteolytic stability. They incorporated a butanoyl, a hexanoyl or a lauroyl group at the N-terminus or at the side chain of a lysine residue placed at each position of the sequence. Their antimicrobial activity and hemolysis depended on the fatty acid length and its position. In particular, lipopeptides containing a butanoyl or a hexanoyl chain exhibited the best biological activity profile. In addition, we observed that the incorporation of the acyl group did not induce the overexpression of defense-related genes in tomato. Best lipopeptides were BP370, BP378, BP381, BP387 and BP389, which were highly active against all the pathogens tested (minimum inhibitory concentration of 0.8 to 12.5 μM), low hemolytic, low phytotoxic and significantly stable to protease degradation. This family of lipopeptides might be promising functional peptides useful for plant protection.

Description: by The PLOS ONE staff

Description: by Fausto Petrelli, Chiara Lazzari, Raffaele Ardito, Karen Borgonovo, Alessandra Bulotta, Barbara Conti, Mary Cabiddu, Jody Filippo Capitanio, Matteo Brighenti, Mara Ghilardi, Luca Gianni, Sandro Barni, Vanesa Gregorc Background Patients with anaplastic lymphoma kinase rearranged (ALK+) non-small cell lung cancer (NSCLC) have a higher risk of developing brain metastases (BMs) than patients with other NSCLC sub-types. ALK inhibitors have activity in BMs due to ALK+ NSCLC. We performed a systematic review of the literature with the aim of assessing the efficacy of ALK inhibitors on BMs. Material and methods A systematic search of the literature was performed using the databases Pubmed, EMBASE, Web of Science, The Cochrane Library, and SCOPUS. Relevant publications reporting activity of ALK inhibitors in NSCLC BMs were retrieved. Data were pooled using the number of events/number of evaluable patients according to fixed or random effect models. Intracranial tumour response was assessed through overall response rate (ORR), disease control rate (DCR: ORR + stable disease rate), median progression-free survival (PFS), and overall survival (OS). The primary endpoint was intracranial overall response rate (IC ORR). Results A total of 1,016 patients with BMs from 21 studies were analysed. In patients receiving ALK inhibitors in the first line setting, the pooled IC ORR was 39.17% (95%CI 13.1–65.2%), while the pooled IC ORR observed in further lines was 44.2% (95%CI 33.3–55.1%). Intracranial disease control rate (IC DCR) was 70.3% and 78.2% in naïve and pre-treated patients, respectively. Patients who had not received brain radiation attained an IC ORR of 49.0%. Conclusions Based on these data, ALK inhibitors are effective in both naive and pre-treated patients with similar IC ORR and IC DCR, irrespective of the line of therapy.

Description: by Steven Denyer, Abhiraj D. Bhimani, Steven Papastefan, Pouyan Kheirkhah, Tania Aguilar, Jack Zakrzewski, Clayton L. Rosinski, Akash S. Patel, Saavan Patel, Victoria Zakrzewski, Akop Seksenyan, Gail S. Prins, Ankit I. Mehta Vertebral compression fractures (VCFs) caused by metastatic malignancies or osteoporosis are devastating injuries with debilitating outcomes for patients. Minimally invasive kyphoplasty is a common procedure used for symptomatic amelioration. However, it fails in treating the underlying etiologies of VCFs. Use of systemic therapy is limited due to low perfusion to the spinal column and systemic toxicity. Localized delivery of drugs to the vertebral column can provide a promising alternative approach. A porcine kyphoplasty model was developed to study the magnetically guided drug delivery of systemically injected magnetic nanoparticles (MNPs). Jamshidi cannulated pedicle needles were placed into the thoracic vertebra and, following inflatable bone tamp expansion, magnetic bone cement was injected to the vertebral body. Histological analysis was performed after intravenous injection of MNPs. Qualitative analysis of harvested tissues revealed successful placement of magnetic cement into the vertebral body. Further quantitative analysis of histological sections of several vertebral bodies demonstrated enhanced accumulation of MNPs to regions that had magnetic cement injected during kyphoplasty compared to those that did not. By modifying the kyphoplasty bone cement to include magnets, thereby providing a guidance stimulus and a localizer, we were successfully able to guide intravenously injected magnetic nanoparticles to the thoracic vertebra. These results demonstrate an in-vivo proof of concept of a novel drug delivery strategy that has the potential to treat the underlying causes of VCFs, in addition to providing symptomatic support.

Description: by Maria Frølund, Arne Wikström, Peter Lidbrink, Waleed Abu Al-Soud, Niels Larsen, Christoffer Bugge Harder, Søren Johannes Sørensen, Jørgen Skov Jensen, Peter Ahrens Background Non-gonococcal urethritis (NGU) is a common syndrome in men. NGU may have several causes, but many cases are caused by sexually transmitted infections that may also cause complications in their female partners. Chlamydia trachomatis and Mycoplasma genitalium are the most common causes of NGU, but in up to 35% of the cases, none of the known viral or bacterial causes are found. Traditionally, pathogens have been detected using various culture techniques that may not identify all species present in the urethra. To address this, we used culture-independent methods for analysis of the male urethral microbiota. Methods This case-control study analysed first void urine samples, collected at STD clinics in Stockholm, Sweden from men with idiopathic urethritis (IU), i.e. negative for Neisseria gonorrhoeae , Chlamydia trachomatis , Mycoplasma genitalium , Ureaplasma urealyticum , Trichomonas vaginalis , adenovirus, and herpes simplex virus type 1 and -2 together with samples from men without urethritis. Forty-six controls and 39 idiopathic urethritis patients were analysed. Results The microbiota was highly diverse: None of the 302 operational taxonomic units (OTUs) found in negative controls and IU patients were found in all of the samples or even in all of the samples in one group. More than 50% of the OTUs were only found in one or two of the total of 85 samples. Still the most dominant 1/6 of the genera constituted 79% of the sequences.Hierarchical clustering in a heatmap showed no specific clustering of patients or controls.A number of IU patient samples were dominated by a single genus previously related to urethritis ( Gardnerella , Haemophilus , Ureaplasma ). Conclusion The male urethra contain a very diverse composition of bacteria, even in healthy controls. NGU may be caused by a number of different bacteria but more studies including a higher number of samples are needed for elucidation of the role of each species.

Description: by Yves Jackson, Adeline Paignon, Hans Wolff, Noelia Delicado Background Undocumented migrants endure adverse living conditions while facing barriers to access healthcare. Evidence is lacking regarding their healthcare needs, notably in regards to chronic diseases. Our goal was to investigate health conditions in undocumented migrants attended in primary care setting. Methods This study was conducted at the primary care outpatient clinic, Geneva University Hospitals, Switzerland. We retrospectively recorded and coded all medical conditions of a random sample of 731 undocumented migrants using the International Classification of Primary Care, 2nd version (ICPC-2). We dichotomized conditions as chronic or non-chronic and considered multimorbidity in the presence of three or more chronic conditions. Results Participants originated from 72 countries and were mainly female (65.5%) with a mean age of 42.4 (standard deviation [SD]: 11.4) years. They presented a mean of 2.9 (SD: 2.1; range: 1–17) health conditions. In multivariable analysis, the number of conditions was associated with female gender (p = 0.011) and older age (p

Description: by Kine Andenæs, Ida G. Lunde, Naiyereh Mohammadzadeh, Christen P. Dahl, Jan Magnus Aronsen, Mari E. Strand, Sheryl Palmero, Ivar Sjaastad, Geir Christensen, Kristin V. T. Engebretsen, Theis Tønnessen Pressure overload of the heart leads to cardiac remodeling that may progress into heart failure, a common, morbid and mortal condition. Increased mechanistic insight into remodeling is instrumental for development of novel heart failure treatment. Cardiac remodeling comprises cardiomyocyte hypertrophic growth, extracellular matrix alterations including fibrosis, and inflammation. Fibromodulin is a small leucine-rich proteoglycan that regulates collagen fibrillogenesis. Fibromodulin is expressed in the cardiac extracellular matrix, however its role in the heart remains largely unknown. We investigated fibromodulin levels in myocardial biopsies from heart failure patients and mice, subjected fibromodulin knock-out (FMOD-KO) mice to pressure overload by aortic banding, and overexpressed fibromodulin in cultured cardiomyocytes and cardiac fibroblasts using adenovirus. Fibromodulin was 3-10-fold upregulated in hearts of heart failure patients and mice. Both cardiomyocytes and cardiac fibroblasts expressed fibromodulin, and its expression was increased by pro-inflammatory stimuli. Without stress, FMOD-KO mice showed no cardiac phenotype. Upon aortic banding, left ventricles of FMOD-KO mice developed mildly exacerbated hypertrophic remodeling compared to wild-type mice, with increased cardiomyocyte size and altered infiltration of leukocytes. There were no differences in mortality, left ventricle dilatation, dysfunction or expression of heart failure markers. Although collagen amount and cross-linking were comparable in FMOD-KO and wild-type, overexpression of fibromodulin in cardiac fibroblasts in vitro decreased their migratory capacity and expression of fibrosis-associated molecules, i.e. the collagen-cross linking enzyme lysyl oxidase, transglutaminase 2 and periostin. In conclusion, despite a robust fibromodulin upregulation in clinical and experimental heart failure, FMOD-KO mice showed a relatively mild hypertrophic phenotype. In cultured cardiac fibroblasts, fibromodulin has anti-fibrotic effects.

Description: by Bruno Rossitto De Marchi, Tonny Kinene, James Mbora Wainaina, Renate Krause-Sakate, Laura Boykin The whitefly, Bemisia tabaci , is a species complex of more than 40 cryptic species and a major agricultural pest. It causes extensive damage to plants mainly by transmitting plant viruses. There is still a lack of genomic data available for the different whitefly species found in Brazil and their bacterial endosymbionts. Understanding the genetic and transcriptomic composition of these insect pests, the viruses they transmit and the microbiota is crucial to sustainable solutions for farmers to control whiteflies. Illumina RNA-Seq was used to obtain the transcriptome of individual whiteflies from 10 different populations from Brazil including Middle East-Asia Minor 1 (MEAM1), Mediterranean (MED) and New World 2 (NW2). Raw reads were assembled using CLC Genomics Workbench and subsequently mapped to reference genomes. We obtained whitefly complete mitochondrial genomes and draft genomes from the facultative bacterial endosymbiont Hamiltonella for further phylogenetic analyses. In addition, nucleotide sequences of the GroEL chaperonin gene from Hamiltonella from different populations were obtained and analysed. There was concordance in the species clustering using the whitefly complete mitogenome and the mtCOI gene tree. On the other hand, the phylogenetic analysis using the 12 ORF’s of Hamiltonella clustered the native species NW2 apart from the exotics MEAM1 and MED. In addition, the amino acid analysis of GroEL chaperonin revealed a deletion only in Hamiltonella infecting NW2 among whiteflies populations analysed which was further confirmed by PCR and Sanger sequencing. The genomic data obtained in this study will aid understanding the functions that Hamiltonella may have in whitefly biology and serve as a reference for further studies regarding whiteflies in Brazil.

Description: by Wen-Kai Yang, An-Di Hsu, Chao-Kai Kang, Ivan Pochou Lai, Pei-Shao Liao, Tsung-Han Lee FXYD proteins are the regulators of sodium-potassium ATPase (Na + /K + -ATPase, NKA). In teleosts, NKA is a primary driving force for the operation of many ion transport systems in the osmoregulatory organs (e.g. intestines). Hence, the purpose of this study was to determine the expression of FXYD proteins and NKA α-subunit in the intestines of two closely related medakas ( Oryzias dancena and O . latipes ), which came from different salinity habitats and have diverse osmoregulatory capabilities, to illustrate the association between NKA and FXYD proteins of two medaka species in response to salinity changes. The results showed that the fxyd12 mRNA was the most predominant in the intestines of both medakas. The association of FXYD12 and NKA in the intestines of the two medaka species was demonstrated via double immunofluorescent staining and co-immunoprecipitation. Upon salinity challenge, the localization of FXYD12 and NKA was similar in the intestines of the two medaka species. However, the expression profiles of intestinal FXYD12 and NKA (mRNA and protein levels), as well as NKA activity differed between the medakas. These results showed that FXYD12 may play a role in modulating NKA activity in the intestines of the two medakas following salinity changes in the maintenance of internal homeostasis. These findings contributed to knowledge of the expression and potential role of vertebrate FXYD12, the regulators of NKA, upon salinity challenge.

Description: by Raissa Prior Migliorini, Felipe Bortolotto Peters, Marina Ochoa Favarini, Carlos Benhur Kasper Information about resource partitioning among small cat species that live in sympatry in South America is fairly incomplete. Knowledge about feeding habits is essential for understanding the role of these predators in the environment, the impact on prey populations, and potential competition among themselves and with other carnivores. This study aimed to describe and compare the diet of four sympatric small cats in the grasslands of southern Brazil. We analysed the stomach contents of 37 Geoffroy’s cats ( Leopardus geoffroyi ), 27 margays ( Leopardus wiedii ), 14 pampas cats ( Leopardus colocola ), and 20 jaguarundis ( Herpailurus yagouaroundi ) obtained as road kill in the Brazilian Pampa in southern Brazil. Small mammals were the most representative class consumed by all cats, followed by Aves, Reptilia, and Amphibia. Some items, such as rodents Cavia aperea , Akodon sp., Oligoryzomys sp. and Passeriformes were consumed by all cat species. Niche overlap varied widely, from 10% (margay x jaguarundi) to 92% (jaguarundi x pampas cat). Niche breadth indicated that jaguarundi were the most specialized of the cats ( B sta = 0.24) in this region, with a diet closely associated to C . aperea . Margay consumed more items associated with arboreal behaviour than other cat species, but consumed more terrestrial items than arboreal ones. The pampas cat consumed mostly terrestrial species associated with open fields. Geoffroy’s cat consumed mammals found in a diversity of habitats, indicating high ecological flexibility. Species with more similarity in diet such as jaguarundi and pampas cat probably present temporal segregation in activity. In conclusion, despite their habitat and diet similarities, these four species explore distinct microhabitats by foraging different prey groups, what favor them to live in sympatry.

Description: by Cagan Urkup, Burcin Bozkaya, F. Sibel Salman The rapid growth of mobile payment and geo-aware systems as well as the resulting emergence of Big Data present opportunities to explore individual consuming patterns across space and time. Here we analyze a one-year transaction dataset of a leading commercial bank to understand to what extent customer mobility behavior and financial indicators can predict the use of a target product, namely the Individual Consumer Loan product. After data preprocessing, we generate 13 datasets covering different time intervals and feature groups, and test combinations of 3 feature selection methods and 10 classification algorithms to determine, for each dataset, the best feature selection method and the most influential features, and the best classification algorithm. We observe the importance of spatio-temporal mobility features and financial features, in addition to demography, in predicting the use of this exemplary product with high accuracy (AUC = 0.942). Finally, we analyze the classification results and report on most interesting customer characteristics and product usage implications. Our findings can be used to potentially increase the success rates of product recommendation systems.

Description: by Amparo Lázaro, Asier R. Larrinaga Seed size is a fundamental life-history trait for plants. A seed number/size trade-off is assumed because the resources invested in reproduction are limited; however, such a trade-off is not always observed. This could be a consequence of the method used for testing it, where the null hypothesis is dictated by common statistical practice, rather than being based on any underlying theory. Alternatively, there might be some population- and species-dependent variables that affect resource availability and, in turn, influence the presence and intensity of this trade-off. Using data on 42 herbs from two communities (lowland and alpine) from Southern Norway, we tested the validity of the classical linear model vs. two previously proposed models, based on resource competition, when assessing the existence of this trade-off at different levels. We also evaluated whether some species- (fruit aggregation, ovules/flower) and population-dependent (pollen limitation) variables could affect this trade-off. Classical linear modelling outperformed the other proposed functional models. Significant seed number/size relationships were negative in single-fruited species, whereas they were positive in species with infructescences of one-seeded fruits. Concordantly, fruit organization was the most influencing variable for the intra-specific trade-off in the lowland community. In the alpine community, species suffering higher pollen limitation showed more strongly negative slopes between seed size and seed number at the fruit/infructescence level. Across species, seed size and number were negatively related, although the relationship was significant in only one of the communities. No evidence of trade-off was found at the plant level. Linear models provide a flexible framework that allows coping with the variability in the seed number/size relationship. The emergence of the intra-specific relationship between seed number and size depends on species- and population-dependent variables, related to resource allocation and the pollination environment.

Description: by Sunitha Kodidela, Sabina Ranjit, Namita Sinha, Carole McArthur, Anil Kumar, Santosh Kumar Cytokines and chemokines circulate in plasma and may be transferred to distant sites, via exosomes. HIV infection is associated with dysregulation of cytokines and chemokines, which subsequently contribute to the pathogenesis of HIV. Alcohol and tobacco exposure, which are prevalent in HIV-infected individuals, may induce changes in the expression of cytokines and chemokines. Therefore, our aim in this study was to quantify plasma exosomal cytokines and chemokines that we expect to exacerbate toxicity or disease progression in HIV-positive drug abusers. We measured the levels of cytokines and chemokines in the plasma and plasma exosomes of 39 patients comprising six groups: HIV-negative and HIV-positive non drug abusers, HIV-negative and HIV-positive alcohol users, and HIV-negative and HIV positive tobacco smokers. We measured six cytokines (TNF-α, IL-1β, IL-8, IL-6, IL-1ra, IL-10) and two chemokines (MCP-1 and RANTES). All were present in exosomes of healthy subjects, but their levels varied between different study groups. HIV-positive alcohol drinkers had higher levels of plasma IL-8 compared to those of HIV-positive non-drinkers. The IL-1ra level was significantly higher in exosomes of non-HIV-infected alcohol drinkers compared to those of HIV-positive alcohol drinkers. Interestingly, the IL-10 level was higher in exosomes compared with their respective plasma levels in all study groups except HIV-positive non-alcohol drinkers. IL-10 was completely packaged in exosomes of HIV-positive smokers. HIV-positive smokers had significantly higher levels of plasma IL-8 compared with HIV-positive non-smokers and significantly higher exosomal IL-6 levels compared with HIV-negative subjects. HIV-positive smokers had significantly increased plasma levels of IL-1ra compared to HIV-positive non-smokers. The MCP-1 levels in the plasma of HIV-positive smokers was significantly higher than in either HIV-positive non-drug abusers or HIV-negative smokers. Overall, the findings suggest that plasma cytokines and chemokines are packaged in exosomes at varying degrees in different study groups. Exosomal cytokines and chemokines are likely to have a significant biological role at distant sites including cells in the brain.

Description: by Mian Zhou, Monowar Aziz, Manhendar Ochani, Weng-Lang Yang, Archna Sharma, Ping Wang Decrease of CD4 T cell numbers causes immunosuppression in sepsis. We previously showed the beneficial role of ghrelin in sepsis. We hypothesize that the protective outcome of ghrelin in sepsis is mediated partially through the restoration of CD4 T cells’ proliferation. Sepsis was induced in mice by cecal ligation and puncture (CLP). The percentage of CD4 T cells in spleen was assessed by flow cytometry and their proliferation was determined by carboxyfluorescein succinimidyl ester (CSFE). Compared to sham mice, the percentages of splenic CD4 T cells were reduced by 20%, 21%, and 29% at day 1, 2 and 3 after CLP, respectively. Human ghrelin was given to 3 day septic mice by s . c . injection at 5 and 24 h after CLP. Treatment with ghrelin restored the loss of CD4 T cells by increasing their proliferation in septic mice. The expression of cyclin D1 and B1 was significantly increased, while the expression of p57 was decreased in ghrelin-treated mice compared to vehicle-treated mice in sepsis. Treatment with human ghrelin significantly increased the p-AKT levels in the spleen compared to vehicle-treated septic mice. Human ghrelin plays an important role in reestablishing the proliferation of CD4 T cells and serves as a promising therapeutic agent in sepsis.

Description: by Ann Machablishvili, Giorgi Chakhunashvili, Khatuna Zakhashvili, Irakli Karseladze, Olgha Tarkhan-Mouravi, Mari Gavashelidze, Tamar Jashiashvili, Lela Sabadze, Paata Imnadze, Rodney S. Daniels, Burcu Ermetal, John W. McCauley Background Influenza epidemiological and virologic data from Georgia are limited. We aimed to present Influenza Like Illness (ILI) and Severe Acute Respiratory Infection (SARI) surveillance data and characterize influenza viruses circulating in the country over three influenza seasons. Methods We analyzed sentinel site ILI and SARI data for the 2014–2017 seasons in Georgia. Patients’ samples were screened by real-time RT-PCR and influenza viruses isolated were characterized antigenically by haemagglutination inhibition assay and genetically by sequencing of HA and NA genes. Results 32% (397/1248) of ILI and 29% (581/1997) of SARI patients tested were positive for influenza viruses. In 2014–2015 the median week of influenza detection was week 7/2015 with B/Yamagata lineage viruses dominating (79%); in 2015–2016—week 5/2016 was the median with A/H1N1pdm09 viruses prevailing (83%); and in 2016–2017 a bimodal distribution of influenza activity was observed—the first wave was caused by A/H3N2 (55%) with median week 51/2016 and the second by B/Victoria lineage viruses (45%) with median week 9/2017. For ILI, influenza virus detection was highest in children aged 5–14 years while for SARI patients most were aged 〉15 years and 27 (4.6%) of 581 SARI cases died during the three seasons. Persons aged 30–64 years had the highest risk of fatal outcome, notably those infected with A/H1N1pdm09 (OR 11.41, CI 3.94–33.04, p