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  • RFLP  (260)
  • Aircraft Design, Testing and Performance
  • Seismology
  • Industrial Chemistry
  • Inorganic Chemistry
  • Springer  (265)
  • 1995-1999  (263)
  • 1950-1954  (2)
  • 1
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    Die Erde im Visier - Geowissenschaften an der Schwelle zum 21. Jahrhundert (1999)
    Springer
    In:  Heidelberg, Springer, vol. 113, no. XVI:, pp. 1-14, (ISBN 1-56670-263-3)
    Details
    Publikationsdatum: 1999
    Schlagwort(e): Handbook of geophysics ; Seismology ; Seismics (controlled source seismology) ; Early warning systems (earthquakes, volcanic eruptions, tsunamis etc.) ; Earthquake hazard ; KTB ; ICDP ; IOcean Drilling Program ; climate ; Antarctica ; Nuclear explosion ; Volcanology ; GeodesyY ; satellites ; remote ; sensing ; gas ; hydrates ; Geothermics ; Energy (of earthquakes) ; potable ; water ; waste ; soils ; evolution ; Geol. aspects ; geotechnics ; Engineering geophys. ; ores
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    BIBLIOGRAFIE SEISMOLOGIE
  • 2
    Digitale Medien
    Digitale Medien
    Genetic linkage map of a wheat×spelt cross (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1163-1170 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key wordsTriticum aestivum ; Triticum spelta ; RFLP ; Genetic map ; QTL
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  We constructed a genetic map of a cross between the Swiss winter wheat (Triticum aestivum L.) variety Forno and the Swiss winter spelt (Triticum spelta L.) variety Oberkulmer. For the linkage analysis,176 polymorphic RFLP probes and nine microsatellites were tested on 204 F5 recombinant inbred lines (RILs) of Forno×Oberkulmer revealing 242 segregating marker loci. Thirty five percent of these loci showed significant (P〉0.05) deviation from a 1 : 1 segregation, and the percentage of Forno alleles ranged from 21% to 83% for individual marker loci. Linkage analysis was performed with the program MAPMAKER using the Haldane mapping function. Using a LOD threshold of 10, we obtained 37 linkage groups. After finding the best order of marker loci within linkage groups by multi-point analysis we assembled the linkage groups into 23 larger units by lowering the LOD threshold. All except one of the 23 new linkage groups could be assigned to physical chromosomes or chromosome arms according to hybridisation patterns of nulli-tetrasomic lines of Chinese Spring and published wheat maps. This resulted in a genetic map comprising 230 marker loci and spanning 2469 cM. Since the analysed population is segregating for a wide range of agronomically important traits, this genetic map is an ideal basis for the identification of quantitative trait loci (QTLs) for these traits.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051181
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  • 3
    Digitale Medien
    Digitale Medien
    An integrated high-density RFLP-AFLP map of tomato based on two Lycopersicon esculentum×L. pennellii F2 populations (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 254-271 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Molecular markers ; Integrated linkage map ; Tomato ; Lycopersicon species ; AFLP ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Two independent F2 populations of Lycopersicon esculentum×L. pennellii which have previously been investigated in RFLP mapping studies were used for construction of a highly saturated integrated AFLP map. This map spanned 1482 cM and contained 67 RFLP markers, 1078 AFLP markers obtained with 22 EcoRI+MseI primer combinations and 97 AFLP markers obtained with five PstI+MseI primer combinations, 231 AFLP markers being common to both populations. The EcoRI+MseI AFLP markers were not evenly distributed over the chromosomes. Around the centromeric region, 848 EcoRI+ MseI AFLP markers were clustered and covered a genetic distance of 199 cM, corresponding to one EcoRI+ MseI AFLP marker per 0.23 cM; on the distal parts 1283 cM were covered by 230 EcoRI+MseI AFLP markers, corresponding to one marker per 5.6 cM. The PstI/MseI AFLP markers showed a more even distribution with 16 PstI/MseI AFLP markers covering a genetic distance of 199 cM around the centromeric regions and 81 PstI/MseI AFLP markers covering a genetic distance of 1283 cM on the more distal parts, corresponding to one marker per 12 and 16 cM respectively. In both populations a large number of loci showed a significant skewed segregation, but only chromosome 10 loci showed skewness that was similar for both populations. This ultra-dense molecular-marker map provides good perspectives for genetic and breeding purposes and map-based cloning.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051231
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  • 4
    Digitale Medien
    Digitale Medien
    Development and utility of cleaved amplified polymorphic sequences (CAPS) and restriction fragment length polymorphisms (RFLPs) linked to the Fom-2 fusarium wilt resistance gene in melon (Cucumis melo L.) (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 453-463 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Cucumis melo ; Molecular markers ; RAPD ; CAPS ; RFLP ; Fusarium oxysporum ; Fusarium resistance ; Marker-assisted selection (MAS)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Fusarium wilt, caused by Fusarium oxysporum Schlecht f. sp. melonis Snyder & Hans, is a worldwide soil-borne disease of melon (Cucumis melo L.). Resistance to races 0 and 1 of Fusarium wilt is conditioned by the dominant gene Fom-2. To facilitate marker-assisted backcrossing with selection for Fusarium wilt resistance, we developed cleaved amplified polymorphic sequences (CAPS) and restriction fragment length polymorphisms (RFLP) markers by converting RAPD markers E07 (a 1.25-kb band) and G17 (a 1.05-kb band), respectively. The RAPD-PCR polymorphic fragments from the susceptible line ’Vedrantais’ were cloned and sequenced in order to construct primers that would amplify only the target fragment. The derived primers, E07SCAR-1/E07SCAR-2 from E07 and G17SCAR-1/G17SCAR-2 from G17, yielded a single 1.25-kb fragment (designated SCE07) and a 1.05-kb fragment (designated SCG17) (the same as RAPD markers E07 and G17), respectively, from both resistant and susceptible melon lines, thus demonstrating locus-specific associated primers. Potential CAPS markers were first revealed by comparing sequence data between fragments amplified from resistant (PI 161375) and susceptible (’Vedrantais’) lines and were then confirmed by electrophoresis of restriction endonuclease digestion products. Twelve restriction endonucleases were evaluated for their potential use as CAPS markers within the SCE07 fragment. Three (BclI, MspI, and BssSI) yielded ideal CAPS markers and were subsequently subjected to extensive testing using an additional 88 diverse melon cultigens, 93 and 119 F2 individuals from crosses of ’Vedrantais’ x PI 161375 and ’Ananas Yokneam’×MR-1 respectively, and 17 families from a backcross BC1S1 population derived from the breeding line ’MD8654’ as a resistance source. BclI- and MspI-CAPS are susceptible-linked markers, whereas the BssSI-CAPS is a resistant-linked marker. The CAPS markers that resulted from double digestion by BclI and BssSI are co-dominant. Results from BclI- and MspI-CAPS showed over 90% accuracy in the melon cultigens, and nearly 100% accuracy in the F2 individuals and BC1S1 families tested. This is the first report of PCR-based CAPS markers linked to resistance/susceptibility for Fusarium wilt in melon. The RFLP markers resulting from probing with a clone-derived 1.05-kb SCG17 PCR fragment showed 85% correct matches to the disease phenotype. Both the CAPS and RFLP markers were co-dominant, easier to score, and more accurate and consistent in predicting the melon phenotype than the RAPD markers from which they were derived.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051257
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  • 5
    Digitale Medien
    Digitale Medien
    Comparative mapping in loblolly and radiata pine using RFLP and microsatellite markers (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 656-662 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Comparative mapping ; RFLP ; Microsatellite markers ; Loblolly pine ; Radiata pine
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Genetic linkage maps were constructed for loblolly pine (Pinus taeda L.) and radiata pine (P. radiata D. Don) using a common set of RFLP and microsatellite markers. The map for loblolly pine combined data from two full-sib families and consisted of 20 linkage groups covering 1281 cM. The map for radiata pine had 14 linkage groups and covered 1223 cM. All of the RFLP probes readily hybridise between loblolly and radiata pine often producing similar hybridisation patterns. There were in total 60 homologous RFLP loci mapped in both species which could be used for comparative purposes. A set of 20 microsatellite markers derived from radiata pine were also assayed; however, only 9 amplified and revealed polymorphic loci in both species. Single-locus RFLP and microsatellite markers were used to match up linkage groups and compare order between species. Twelve syntenic groups were obtained each consisting of from 3 to 9 homologous loci. The order of homologous loci was colinear in most cases, suggesting no major chromosomal rearrangements in the evolution of these species. Comparative mapping between loblolly and radiata pine should facilitate genetic research in both species and provide a framework for mapping in other pine species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051281
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  • 6
    Digitale Medien
    Digitale Medien
    Breeding for ”low-gossypol seed and high-gossypol plants” in upland cotton. Analysis of tri-species hybrids and backcross progenies using AFLPs and mapped RFLPs (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 1233-1244 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Cotton ; Gossypol ; Wide hybridization ; Backcrossing ; DNA markers ; AFLP ; RFLP ; Introgression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  This work aims at breeding upland cotton [Gossypium hirsutum L., 2(AD)1 genome] with a reduced level of gossypol in the seeds for optimal food and feed uses, and a high gossypol level in the remaining organs for resistance to pests. Two tri-species Gossypium hybrids, (G. thurberi–G. sturtianum–G. hirsutum and G. hirsutum–G. raimondii–G. sturtianum) including G. sturtianum (2C1) as a donor, G. thurberi (2D1) and G. raimondii (2D5) as a bridge species, were created. Recurrent selection initiated with these tri-species hybrids produced backcross (BC) progenies expressing the ”low-gossypol seed and high-gossypol plant” trait at different levels. We used AFLP markers to assess the genetic similarity among the germplasm and RFLP probes to tag the introgression of specific chomosome segments from the parental species. Five pairs of AFLP primers generated 477 fragments, among which 417 (87.4%) were polymorphic. The genetic similarity between the upland cotton and the wild species ranged from 29.5 to 43.2%, while similarity reached 80% between upland cotton and BC3 plants. Introgression of species-specific AFLPs was evident from all the parental species and confirmed the hybrid origin of the analyzed progenies. Southern-blot analysis based on 49 RFLP probes allowed us to trace the introgression of parental DNA segments in the tri-species hybrids and in three generations of backcross. Introgression was evident from 11, 8 and 7 linkage groups of G. sturtianum, G. raimondii and G. thurberi respectively. The types of introgression revealed by RFLP probes are discussed, and breeding schemes to enhance recombination are proposed. The ability to trace DNA segments of known chromosomal locations from the donor G. sturtianum through segregating generations is a starting point to map the ”low-gossypol seed and high-gossypol plant” traits.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051329
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  • 7
    Digitale Medien
    Digitale Medien
    Molecular mapping of two dwarfing genes differing in their GA response on chromosome 2H of barley (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 670-675 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Dwarfing genes ; GA insensitivity ; Genetic mapping ; RFLP ; Hordeum vulgare
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract   The two recessive dwarfing mutants gai (GA-ins) and gal (GA-less), differing in their response to exogenously applied gibberellic acid (GA3), were mapped in the centromere region and on the long arm, respectively, of the barley chromosome 2H. The gene gai, which determines reduced plant height and GA insensitivity pleiotropically, was found to co-segregate with the two RFLP markers Xmwg2058 and Xmwg2287. Both markers are known to map close to the centromere. The GA-sensitive dwarfing gene gal was found to be linked to the three co-segregating RFLP markers Xmwg581, Xmwg882 and Xmwg2212 (proximal) and XksuG5 (distal) by 3.6 and 9.5. cM, respectively. The distance between the two mutant loci was estimated to be about 55 cM. Homoeologous relationships between the dwarfing genes within the Triticeae are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051283
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  • 8
    Digitale Medien
    Digitale Medien
    Variable genome composition in Triticum×Leymus amphiploids (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 1087-1093 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Amphiploid ; Triticum ; Leymus ; FISH ; RFLP ; Ribosomal genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Three different amphiploid lines originated from crosses between wheat (Triticum L.) and lymegrass (Leymus Hochst.) were analysed by fluorescence in situ hybridization (FISH) using total genomic DNA and 18S.26S ribosomal genes (rDNA). Based on the genomic probes, these lines were the same in that they all were allohexaploids (2n=6x=42) containing 30 wheat and 12 lymegrass chromosomes. The ribosomal gene mapping further identified species origin of the chromosomes, whereby the lymegrass parent was undoubtedly L. mollis and the wheat was likely to be a Triticum species having the AB genomes. This rDNA mapping was also able to reveal differences in the genome composition among these lines, and such differences were mainly in the wheat nucleolar organizing regions (Nor). The first line (M) had two Nor-B1 (1BS), the second line (G) had one Nor-B1 (1BS), two Nor-B2 (6BS) and two Nor-A1 (1AS), whereas the third line (U) had the same Nor loci as the second line but two sites each. The wheat ribosomal genes were variably expressed depending on the lines, but the lymegrass loci appeared inactive. All three lines had the same Nor loci belonging to L. mollis, two Nor-m1 and two Nor-m2. Analysis of restriction fragment length polymorphism (RFLP) of the rDNA confirmed the identity of L. mollis parent in all three lines and verified the differences in the wheat ribosomal genes among them. These amphiploids were, however, similar in their restriction profiles, therefore indicating common origin. The molecular and cytogenetic evidence here suggested that these annual, fully fertile amphiploids that originated from the same crosses 40 years ago became genetically differentiated and fixed in stable forms.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051313
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  • 9
    Digitale Medien
    Digitale Medien
    RFLP markers associated with soybean cyst nematode resistance and seed composition in a ‘Peking’בEssex’ population (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 356-364 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words SCN ; RFLP ; QTL ; Molecular marker ; Soybean
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Soybean cyst nematode (SCN), Heterodera glycines Ichinohe, causes severe damage to soybean [Glycine max (L.) Merr] throughout North America and worldwide. Molecular markers associated with loci conferring SCN resistance would be useful in breeding programs using marker-assisted selection (MAS). In this study, 200 F2:3 families derived from two contrasting parents, SCN-resistant ‘Peking’ with relatively low protein and oil concentrations, and SCN-susceptible ‘Essex’ with high protein and oil concentrations, were used to determine loci underlying the SCN resistance and seed composition. Three different SCN Race isolates (1, 3, and 5) were used to screen both parents and F2:3 families. The parents were surveyed with 216 restriction fragment length polymorphism (RFLP) probes with five different restriction enzymes. Fifty-six were polymorphic and contrasted with trait data from bioassays to identify molecular markers associated with loci controlling resistance to SCN and seed composition. Five RFLP markers, A593 and T005 on linkage group (LG) B, A018 on LG E, and K014 and B072 on LG H, were significantly linked to resistance loci for Race 1 isolate, which jointly explained 57.7% of the total phenotypic variation. Three markers (B072 and K014, both on LG H; T005 on LG B) were associated with resistance to the Race 3 isolate and jointly explained 21.4% of the total phenotypic variation. Two markers (K011 on LG I, A963 on LG E) associated with resistance to the Race 5 isolate together explained 14.0% of the total phenotypic variation. In the same population we also identified two RFLP markers (B072 on LG H, B148 on LG F) associated with loci conferring protein concentration, which jointly explained 32.3% of the total phenotypic variation. Marker B072 was also linked to loci controlling the concentration of seed oil, which explained 21% of the total phenotypic variation. Clustering among quantitative trait loci (QTLs) conditioning resistance to different SCN Race isolates and seed protein and oil concentrations may exist in this population. We believe that markers located near these QTLs could be used to select for new SCN resistance and higher levels of seed protein and oil concentrations in breeding improved soybean cultivars.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051080
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  • 10
    Digitale Medien
    Digitale Medien
    Detection of quantitative trait loci on chromosome 5R of rye (Secale cereale L.) (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1087-1090 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Quantitative trait loci ; Genetic mapping ; RFLP ; Agronomic characters ; Secale cereale L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Progenies of an F2 mapping population were analyzed for quantitative traits to detect QTLs by using marker information from F2 plants for chromosome 5R. The mapping population was segregating for the major dwarfing gene Ddw1 and the gene Hp1 for hairy peduncle. The only QTL determining plant height was located between HP1 and Ddw1 on the distal part of chromosome 5RL. At the same position a QTL for peduncle length was found, and this trait was closely related to plant height (r=0.895). Since Hp1 and Ddw1 are dominant marker loci, no dominance effect could be estimated. The QTLs for spike length and the number of florets were located near the centromere on 5RL. These two traits were correlated with r=0.824 and showed partial dominance, but these traits were not correlated to plant height and peduncle length. Homoeologous relationships between the QTLs mapped for the first time in rye and those mapped in other Triticeae members are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051171
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  • 11
    Digitale Medien
    Digitale Medien
    Linkage disequilibrium among modern sugarcane cultivars (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 1053-1060 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Sugarcane cultivars ; Linkage disequilibrium ; Foundation bottleneck ; Interspecific hybrids ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Modern sugarcane cultivars are derived from a few interspecific hybrids created early in this century. Linkage disequilibrium was investigated in a population of 59 cultivars representing the most important commercial clones bred in Mauritius as well as a few old cultivars involved in their genealogy. Thirty-eight probes scattered over the sugarcane genome map were used to reveal RFLPs. Forty-two cases of bilocus associations were observed involving a total of 33 loci. Most of them are separated by less than 10 cM. All the corresponding allele couples were found in at least 1 of the originally created cultivars, suggesting that they depict ancient associations. This global disequilibrium is interpreted as the result of the foundation bottleneck related to the first interspecific crosses; the preferential allele associations thus created have been maintained through subsequent crosses when the loci were closely linked. This phenomenon is likely also to apply to genes of agricultural interest. A practical consequence is that markers can be used to track known QTLs in modern breeding materials without the necessity to repeatedly study segregating progenies. This structure gives high value to the correlation between molecular markers and agricultural traits among cultivars.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051414
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  • 12
    Digitale Medien
    Digitale Medien
    Characterization of S tester lines in Brassica oleracea: polymorphism of restriction fragment length of SLG homologues and isoelectric points of S-locus glycoproteins (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1329-1334 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Brassica oleracea ; S haplotype ; Self-incompatibility ; RFLP ; IEF
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Forty three S tester lines of Brassica oleracea were characterized using DNA and protein gel-blotting analyses. DNA gel-blot analysis of HindIII-digested genomic DNA with class-I and class-II SLG probes revealed that 40 lines could be classified as class-I S haplotypes while three lines could be classified as class-II S haplotypes. The band patterns in the S tester lines were highly polymorphic. Although the S tester lines typically showed two bands corresponding to SLG and SRK in the analysis with the class-I SLG probe, only one band was observed in the S 24 homozygote. This band was identified as SRK, suggesting that this haplotype has no class-I SLG band. In the analysis using the class-II SLG probe, one plant yielded a different band pattern from the known class-II haplotypes, S 2 , S 5 and S 15 . Unexpectedly, this plant was reciprocally cross-incompatible with the S 2 haplotype. Therefore, it was designated as S 2-b . We found an S 13 haplotype having a restriction fragment length polymorphism different from that of the S 13 homozygotes of the S tester line. These findings indicate that S homozygous lines with the same S specificity do not necessarily show the same band pattern in the DNA gel-blot analysis. Soluble stigma proteins of 32 S homozygotes were separated by isoelectric focusing and detected using anti-S 22 SLG antiserum. S haplotype-specific bands were detected in 27 S homozygotes but not in five S homozygotes, including the S 24 homozygote. This is consistent with the observation that the S 24 haplotype had no SLG band.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051199
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  • 13
    Digitale Medien
    Digitale Medien
    Phylogenetic analysis of Acacia (Mimosaceae) as revealed from chloroplast RFLP data (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 291-298 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words DNA ; Molecular evolution ; Parsimony ; PAUP ; Phylogeny ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Chloroplast DNA of 22 species of Acacia (Tourn.) Miller was digested with ten restriction endonucleases, Southern-blotted and probed with cloned fragments covering the chloroplast genome of tobacco (Nicotiana tabacum L.). Phyletic and phenetic analyses of the resulting 176 polymorphic bands recorded among the 22 species were performed. The phylogram was reconstructed using heuristic search and Wagner parsimony. The resulting most parsimonious consensus phylogram displayed three major phyletic lineages, consistent with the previously established three subgenera of Acacia. The 10 species of subgenus Acacia and the 6 species of subgenus Heterophyllum formed two monophyletic sister clades. The 5 species of subgenus Aculeiferum studied and Acacia albida (Syn. Faidherbia albida) grouped together and were basal to the clades of subgenera Acacia and Heterophyllum. The phylogram indicated that subgenus Heterophyllum diverged earlier from subgenus Aculeiferum than did subgenus Acacia; however, the phenogram indicated the reverse. The study indicated that A. nilotica and A. farnesiana are sister species, though A. nilotica is Afro-Asiatic and A. farnesiana is American. The phenogram separated the three subgenera in agreement with the phylogram, but the two dendrograms differed regarding the topologies of the species and the distance of evolution between subgenera Acacia and Heterophyllum.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051071
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  • 14
    Digitale Medien
    Digitale Medien
    Mapping of Ve in tomato: a gene conferring resistance to the broad-spectrum pathogen, Verticillium dahliae race 1 (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 315-319 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Linkage analysis ; Mapping populations ; Introgression Lines ; RFLP ; Host range
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The soil-borne fungi Verticillium spp. cause vascular wilt disease in a wide range of crop plants. In tomato, resistance to Verticillium dahliae race 1 is conferred by a single dominant gene, Ve. Previous efforts to map Ve in tomato have yielded confusing results, locating it on different chromosomes, which subsequently raised the possibility that Verticillium resistance may be controlled by a number of loci. We used three different mapping populations to obtain an unambiguous map location of Ve: a recombinant inbred (RI) line population; an F2 population segregating for Verticillium resistance; and a population of 50 introgression lines (IL). In all of the mapping populations Ve was positioned on the short arm of chromosome 9 tightly linked to the RFLP marker GP39. This linkage was confirmed by screening for GP39 in different breeding lines with known resistance or susceptibility to Verticillium. A perfect match was found between GP39 and the Verticillium response of the lines, indicating the potential of GP39 in the rapid detection of Verticillium resistance and as a starting point for map-based cloning of Ve. This approach is particularly relevant for Verticillium dahliae race 1, since in the present work we also show that the isolate that infects tomato is responsible for wilt disease in other important crop plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051075
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  • 15
    Digitale Medien
    Digitale Medien
    Towards a saturated sorghum map using RFLP and AFLP markers (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 320-328 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Sorghum ; RFLP ; AFLP ; Genetic linkage map
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A near-saturated sorghum genetic linkage map was produced using RFLP, AFLP and morphological markers. First a composite, essentially RFLP-based genetic linkage map was obtained from analyses of two recombinant inbred populations. This map includes 343 loci for 11 linkage groups spanning 1352 cM. Since this map was constructed with many previously mapped heterologous probes, it offers a good basis for synteny studies. Separately, an AFLP map was obtained from the analysis of 168 bands revealed from 12 primer pair combinations. It includes 137 loci for 11 linkage groups spanning 849 cM. Taking into account the different data sets, we constructed a combined genetic linkage map including 443 loci spanning 1899 cM. Two main features are to be noted: (1) the distribution of AFLPs along the genome is not uniform; (2) an important stretching of the former core map is induced after adding the AFLPs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051076
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  • 16
    Digitale Medien
    Digitale Medien
    Integration of dinucleotide microsatellites from hexaploid bread wheat into a genetic linkage map of durum wheat (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1202-1207 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Wheat microsatellites ; Linkage map ; RFLP ; T. turgidum ssp. durum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Seventy nine microsatellite markers from hexaploid bread wheat (T. aestivum L.) were integrated into a genetic linkage map of durum wheat (T. turgidum ssp. durum (Desf.) Huns.) created by RFLP segregation data from a population of 65 recombinant inbred lines. The results indicate a relatively even distribution of microsatellite loci and demonstrate that microsatellite markers from hexaploid wheat provide an excellent source of molecular markers for use in the genetics and breeding of durum wheat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051185
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  • 17
    Digitale Medien
    Digitale Medien
    Molecular investigation of the genetic base of sugarcane cultivars (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 171-184 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Saccharum officinarum ; Sugarcane cultivars ; Polyploid ; Genetic diversity ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Molecular diversity was analysed among 162 clones of sugarcane using DNA restriction fragment length polymorphism (RFLP). One hundred and nine of them were modern cultivars of interspecific origin; most of them were bred in Barbados or in Mauritius. Fifty three were from Saccharum officinarum species, which is the major source of genes in modern cultivars, prevailing over the part of the genome incorporated from the wild species Saccharum spontaneum. Twelve low-copy nuclear DNA probes scattered over the genome were used in combination with one or two restriction enzymes. A total of 399 fragments was identified, 386 of which were polymorphic. Each sugarcane clone displayed a high number of fragments per probe/enzyme combination, illustrating the polyploid constitution of the genome. Among the S. officinarum clones, those from New Guinea had the largest variability and encompassed that present among clones collected from the Indonesian Islands and those known to have been involved in the parentage of modern cultivars. This is in agreement with the hypothesis that New Guinea is the centre of origin of this species. The clones from New Caledonia formed a separate group and could correspond to S. officinarum clones modified through introgression with other members of the ‘Saccharum complex’. Despite the low number of S. officinarum clones used for breeding cultivars, more than 80% of the markers present in the whole S. officinarum sample were also found in modern cultivars due probably to a high heterozygosity related to polyploidy. Among the cultivars, the two main groups, originating from Barbados and Mauritius, were clearly separated. This appeared essentially due to S. spontaneum alleles present in Mauritian cultivars and absent in Barbadan ones, probably in relation to the regular use of early generation interspecific hybrids in the breeding program employed in Mauritius.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051222
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  • 18
    Digitale Medien
    Digitale Medien
    A composite map of expressed sequences and phenotypic traits of the sunflower (Helianthus annuus L.) genome (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 218-234 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Sunflower ; Linkage mapping ; cDNA ; RFLP ; Phenotypic traits ; Composite mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A map of the sunflower genome, based on expressed sequences and consisting of 273 loci, was constructed. The map incorporates data from seven F2 populations, for a total of 1115 individuals. Two hundred and fourty five loci corresponding to 170 anonymous cDNA markers and four loci for morphological markers were mapped. We also mapped 18 loci corresponding to previously described genes or to sequences obtained through homology cloning. The unit maps vary from 774 cM to 1060 cM, with an average value of 14 major linkage groups. The integrated map is arranged in 17 major linkage groups including 238 loci, plus four small segments with 2–5 marker loci; and covers 1573 cM with an overall average marker interval of 7 cM. Thirty five percent of the markers were dominant in nature and 30% showed inter-linkage group duplication without any indication of homoeologous linkage groups. Evidence is provided for the independence of two distinct fertility restoration genes, for the presence of two loosely linked branching loci, and for marker tightly linked to the Rf1 restoration locus. This map provides an efficient tool in breeding applications such as disease-resistance mapping, QTL analyses and marker-assisted selection.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051228
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  • 19
    Digitale Medien
    Digitale Medien
    Identification and mapping of a gene conferring resistance to the spot form of net blotch (Pyrenophora teres f maculata) in barley (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 323-327 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Hordeum vulgare ; Disease resistance ; Genetic mapping ; RFLP ; QTL
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Spot form of net blotch (SFNB) (Pyrenophora teres f maculata) is an economically damaging foliar disease of barley in many of the world’s cereal growing areas. The development of SFNB-resistant cultivars may be accelerated through the use of molecular markers. A screen for SFNB resistance in 96 lines identified four new sources of resistance, including a feed variety, ‘Galleon’, for which a fully mapped doubled haploid population was available. Segregation data indicated SFNB resistance was conferred by a single gene in the ‘Galleon’בHaruna Nijo’ cross, positioned on the long arm of chromosome 7H. This gene is designated Rpt4 and is flanked by the RFLP loci Xpsr117(D) and Xcdo673 at distances of 6.9 cM and 25.9 cM, respectively. The marker Xpsr117(D) was validated using another population segregating for Rpt4, correctly predicting SFNB resistance with more than 90% accuracy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051239
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  • 20
    Digitale Medien
    Digitale Medien
    RFLP mapping of BaYMV resistance gene rym3 in barley (Hordeum vulgare) (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 727-732 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Hordeum vulgare ; BaYMV ; Resistance gene ; RFLP ; Mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The rym3 (formerly designated ym3) gene conferring resistance to barley yellow mosaic virus (BaYMV) is effective against all strains of the virus but up to now has not been mapped to any chromosome. We performed a linkage analysis, using DNA extracted from individually harvested mature leaves of 153 F2 plants derived from a cross between BaYMV-resistant cv ’Ishuku Shirazu’ carrying rym3 and susceptible cv ’Ko A’. Additionally, the F3 lines derived from F2 plants were grown in the BaYMV-infested field and examined for their reaction to BaYMV. Our results indicated that rym3 is located on the short arm of chromosome 5H and flanked by RFLP markers MWG28and ABG705A at distances of 7.2 and 11.7 cM, respectively. The chromosomal configuration estimated by DNA markers around rym3 and the utilization of these molecular markers for pyramiding with the BaYMV resistance genes in barley breeding programs are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051290
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  • 21
    Digitale Medien
    Digitale Medien
    A new polymorphism in the 5' flanking region of the human interleukin (IL)-4 gene (1999)
    Springer
    Immunogenetics 49 (1999), S. 738-739 
    Details
    ISSN: 1432-1211
    Schlagwort(e): Key words Interleukin (IL)-4 ; Promoter ; Polymorphism ; RFLP ; Linkage disequilibrium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002510050677
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  • 22
    Digitale Medien
    Digitale Medien
    Molecular identification of co-occurring Cortinarius and Dermocybe species from southeastern Australian sclerophyll forests (1999)
    Springer
    Mycorrhiza 9 (1999), S. 85-90 
    Details
    ISSN: 1432-1890
    Schlagwort(e): Key words PCR ; RFLP ; ITS sequence analysis ; Ectomycorrhizal fungi ; Cortinarius taxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The ability of restriction fragment length polymorphism (RFLP) analysis of the rDNA internal transcribed spacer (ITS) region to discriminate 10 co-occurring Cortinarius and Dermocybe species at a southeastern Australian sclerophyll forest site was assessed. Using the basidiomycete-specific primers ITS1F and ITS4B, some taxa were separated on the basis of individual RFLP patterns derived using the restriction endonucleases Hae III or Hinf I. Combined data from both endonucleases were, however, required to separate all taxa [Dermocybe austro-veneta Clel. (Moser & Horak), C. rotundisporus Clel. & Cheel, C. archeri Berk., C. sinapicolor Clel., C. violaceus (L.: Fr.) S.F.Gray, C. radicatus Clel. and four morphologically-distinct, but unidentified Cortinarius spp.]. ITS sequence comparisons confirmed that D. austro-veneta belongs in Dermocybe, that C. rotundisporus is correctly placed in subgenus Phlegmacium, and suggest that Australian C. violaceus collections are not conspecific with northern hemisphere C. violaceus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s005720050004
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  • 23
    Digitale Medien
    Digitale Medien
    Leek Proliferation: A New Phytoplasma Disease in the Czech Republic and Italy (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 487-493 
    Details
    ISSN: 1573-8469
    Schlagwort(e): electron microscopy ; molecular identification ; PCR ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract During the summer 1996, twelve of twenty-eight leek plants located in a garden near České Budějovice, South Bohemia exhibited symptoms typical of diseases associated with phytoplasmas. In summer 1998 similar symptoms were detected in leek plants in a field used for seed production located in Romagna, North Italy. In both cases the plants were established in the spring of the previous year. Plants showed flower abnormalities: stamen elongation, anther sterility, pistil proliferation, as well as poor, if any, seed production. Phytoplasma-like structures were detected by scanning and transmission electron microscopy in phloem sieve elements in the Czech diseased plants, but not in healthy ones. Nested-PCR amplifications of extracted DNA with phytoplasma-specific oligonucleotide primer pairs confirmed the presence of phytoplasmas in these plants at low concentrations. Restriction fragment length polymorphism analyses of amplified ribosomal sequences allowed the identification of detected phytoplasmas: all the samples from the Czech Republic contained aster yellows related phytoplasmas (16SrI-B) while in the Italian samples aster yellows related phytoplasmas (16SrI-B) together with stolbur related phytoplasmas (16SrXII-A) were identified. This is the first report of detection and identification of a phytoplasma disease of leek in the Czech Republic and Italy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008745206438
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  • 24
    Digitale Medien
    Digitale Medien
    Gene and Genotypic Diversity of Phytophthora cinnamomi in South Africa and Australia Revealed by DNA Polymorphisms (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 667-680 
    Details
    ISSN: 1573-8469
    Schlagwort(e): asexual reproduction ; mating types ; oomycetes ; origin ; RAPD ; RFLP ; population genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Phytophthora cinnamomi isolates from South Africa and Australia were compared to assess genetic differentiation between the two populations. These two populations were analysed for levels of phenotypic diversity using random amplified polymorphic DNAs (RAPDs) and gene and genotypic diversity using restriction fragment length polymorphisms (RFLPs). Sixteen RAPD markers from four decanucleotide Operon primers and 34 RFLP alleles from 15 putative loci were used. A few isolates from Papua New Guinea known to posses alleles different from Australian isolates were also included for comparative purposes. South African and Australian P. cinnamomi populations were almost identical with an extremely low level of genetic distance between them (Dm=0.003). Common features for the two populations include shared alleles, low levels of phenotypic/genotypic diversity, high clonality, and low observed and expected levels of heterozygosity. Furthermore, relatively high levels of genetic differentiation between mating type populations (Dm South Africa=0.020 and Dm Australia=0.025 respectively), negative fixation indices, and significant deviations from Hardy–Weinberg equilibrium, all provided evidence for the lack of frequent sexual reproduction in both populations. The data strongly suggest that both the South African and Australian P. cinnamomi populations are introduced.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008755532135
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  • 25
    Digitale Medien
    Digitale Medien
    Differentiation of Rhizoctonia AG-D Isolates from Turfgrass into Subgroups I and II Based on rDNA and RAPD Analyses (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 835-846 
    Details
    ISSN: 1573-8469
    Schlagwort(e): turfgrass ; binucleate ; RFLP ; ITS
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Binucleate Rhizoctonia anastomosis group (AG) D is the cause of rhizoctonia-patch and elephant-footprint diseases of zoysiagrass, and winter-patch disease of bentgrass. Rhizoctonia AG-D is also known as the causal pathogen of other diseases such as sharp-eye-spot of cereals, foot-rot of cereals and winter-stem-rot of mat rush. Isolates of AG-D have been divided into the two subgroups AG-D (I) and AG-D (II), based on the results of cultural characteristics and pathogenicity tests. Isolates obtained from zoysiagrass exhibiting symptoms of rhizoctonia-patch disease, from bentgrass with winter-patch disease, from wheat with foot-rot disease, and from mat rush with winter-stem-rot disease were reported to belong to subgroup AG-D (I). On the other hand, isolates obtained from zoysiagrass with elephant-footprint disease were assigned to subgroup AG-D (II). To confirm the existence of these two subgroups in AG-D, the genetic structure of AG-D isolates from turfgrass and other crops was compared. RFLP analysis of the ITS region from rDNA after digestion with the restriction enzymes EcoRI, HaeIII, HhaI, HinfI, and MboI separated AG-D isolates into two groups corresponding to AG-D (I) and AG-D (II). Furthermore, other AGs except AG-Q (AGs-A, Ba, Bb, C, E, F, G, I, K, L, O, P, and R. solani AG1-IC) did not have the same patterns that were seen for the two AG-D subgroups. AG-Q isolates from bentgrass showed the same patterns as AG-D (I). The results of the RAPD analysis also revealed the existence of two groups that corresponded to AG-D (I) and AG-D (II). These analyses revealed that Rhizoctonia AG-D isolates from turfgrass could be divided into two subgroups consistent with those based on cultural characteristics and pathogenicity. In addition, isolates of foot-rot disease of wheat and isolates of winter-stem-rot disease of mat rush whose cultural characteristics were the same as those of AG-D (I) also showed similar RFLP and RAPD patterns to those of AG-D (I) isolates from turfgrass.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008736404263
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  • 26
    Digitale Medien
    Digitale Medien
    Protistan community structure: molecular approaches for answering ecological questions (1999)
    Springer
    Hydrobiologia 401 (1999), S. 215-227 
    Details
    ISSN: 1573-5117
    Schlagwort(e): 18S rRNA ; protistan biogeography ; protists ; RFLP ; oligonucleotide probe ; protistan community structure ; in-situ hybridization ; microbial ecology ; molecular ecology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1003721923719
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  • 27
    Digitale Medien
    Digitale Medien
    Identification of a RAPD marker linked to a brown planthopper resistance gene in rice (1999)
    Springer
    Euphytica 107 (1999), S. 23-28 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Bph-1 ; linkage analysis ; mapping ; RAPD ; RFLP ; rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract We report the tagging of a brown planthopper (BPH) resistance gene (Bph–1) in rice using RAPD and RFLP markers. The Korean rice variety ‘Gayabyeo’ has dominant duplicate genes including Bph–1 conferring resistance to biotype 1 of BPH. Bulked segregant RAPD analysis was employed for rapid identification of DNA markers linked to resistance genes. For tagging these two genes, an F2F3 population from a ‘Gayabyeo’ × ‘Nagdongbyeo’ cross was developed and evaluated for BPH resistance. Three bulked DNAs from two groups of homozygous BPH resistant (each for Bph–1 and the other unknown gene) and homozygous susceptible F2 plants were analyzed by RAPD using 140 random oligomers. One primer, OPD–7 yielded a 700-bp fragment that was present in Gayabyeo and resistant F2 plants (homozygous for Bph-1 locus) but absent in Nagdongbyeo and susceptible F2 plants. Cosegregation of this marker with Bph-1 was verified using an F2 population segregating for Bph-1. Chromosomal regions surrounding the Bph-1 were examined with additional RFLP and microsatellite markers on chromosome 12 to define the location of the RAPD marker and Bph-1. Use of this RAPD marker could facilitate early selection of resistant lines for BPH.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1003506830735
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  • 28
    Digitale Medien
    Digitale Medien
    Molecular cytogenetic identification of wheat-Elymus tsukushiense introgression lines (1999)
    Springer
    Euphytica 107 (1999), S. 217-224 
    Details
    ISSN: 1573-5060
    Schlagwort(e): C-banding ; chromosome additions ; chromosome substitution ; Elymus tsukushiense ; homoeology ; in situ hybridization ; RFLP ; Roegneria kamoji ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Elymus tsukushiense Honda (syn. Roegneria kamoji C. Koch) (2n = 6x = 42, StsStsHtsHtsYtsYts) is a hexaploid species, distantly related to bread wheat Triticum aestivum L. em Thell (2n = 6x = 42, AABBDD). Apart from the delineation of evolutionary relationships, this species is a potential source of resistance to scab, a devastating disease of wheat caused by Fusarium graminearum Schw. A standard C-banded karyotype was established identifying all 21 chromosome pairs of E. tsukushiense. By using C-banding and genomic in situ hybridization analyses, three wheat-E. tsukushiense chromosome addition lines, one ditelosomic addition line, and one disomic substitution line were identified in BC2 progenies from wheat × E. tsukushiense hybrids. Twenty DNA markers specific for the seven homoeologous groups of the Triticeae were used to determine the homoeology of the added E. tsukushiense chromosomes. The E. tsukushiense chromosomes in the addition lines NAU702, NAU703, and NAU701 were identified as belonging to homoeologous groups 1, 3, and 5, and thus, were designated as 1Ets#1, 3Ets#1, and 5Ets#1, respectively. NAU751 was identified as a disomic substitution line with chromosome 3A of wheat replaced by chromosome 3Ets#1. Line NAU702 has a high level of resistance to scab and will be used in chromosomal engineering and development of improved wheat germplasm for scab resistance breeding.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1003686014905
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  • 29
    Digitale Medien
    Digitale Medien
    RFLP- and physical mapping of resistance gene homologues in rice (O. sativa) and Barley (H. vulgare) (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 509-520 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Resistance gene ; RFLP ; YAC contig ; Fragmentation ; Gene cluster
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The deduced peptide sequences of 25 gene fragments of NBS-LRR resistance (R) gene homologues from rice and barley and of characterized R genes were compared, revealing a string of six conserved motifs. Mapping of the R-gene candidates in rice showed linkage to genes conferring race-specific resistance to rice blast (Pi-k, Pi-f and Pi-1) and bacterial blight disease (Xa-1, Xa-3 and Xa-4), in barley to powdery mildew (Mla) and the rust fungus (Rpg1). In rice four mixed clusters were detected, each harboring at least two highly dissimilar NBS-LRR genes. A YAC-contig was established for one of these mixed clusters. YAC fragmentation experiments revealed the presence of at least five NBS-LRR genes within 200 kb in head-to-tail orientation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051099
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  • 30
    Digitale Medien
    Digitale Medien
    Quantitative trait loci associated with resistance to Fusarium head blight and kernel discoloration in barley (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 561-569 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Fusarium head blight ; Scab ; Kernel discoloration ; Black point ; Kernel blight ; Deoxynivalenol (DON) ; RFLP ; Barley
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Resistance to Fusarium head blight (FHB), deoxynivalenol (DON) accumulation, and kernel discoloration (KD) in barley are difficult traits to introgress into elite varieties because current screening methods are laborious and disease levels are strongly influenced by environment. To improve breeding strategies directed toward enhancing these traits, we identified genomic regions containing quantitative trait loci (QTLs) associated with resistance to FHB, DON accumulation, and KD in a breeding population of F4:7 lines using restriction fragment length polymorphic (RFLP) markers. We evaluated 101 F4:7 lines, derived from a cross between the cultivar Chevron and an elite breeding line, M69, for each of the traits in three or four environments. We used 94 previously mapped RFLP markers to create a linkage map. Using composite interval mapping, we identified 10, 11, and 4 QTLs associated with resistance to FHB, DON accumulation, and KD, respectively. Markers flanking these QTLs should be useful for introgressing resistance to FHB, DON accumulation, and KD into elite barley cultivars.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051269
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  • 31
    Digitale Medien
    Digitale Medien
    QTL mapping of resistance to Sporisorium reiliana in maize (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 593-598 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Keywords Maize ; Head smut ; Sporisorium reiliana ; QTL ; RFLP ; Ustilago maydis ; Puccinia sorghi
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  We mapped and characterized quantitative trait loci (QTL) for resistance to Sporisorium reiliana. A population of 220 F3 families produced from the cross of two European elite inbreds (D32, D145) was evaluated with two replications at a French location with high natural incidence of S. reiliana and at a Chinese location employing artificial inoculation. The 220 F3 families were genotyped with 87 RFLP and seven SSR markers. Using composite interval mapping, we identified two different sets of 3 and 8 QTL for the French and the Chinese locations explaining 13% and 44% of respectively. Individual QTL explained up to 14% of σ^2 p. The 11 QTL mapped to eight maize chromosomes and displayed mostly additive or partial dominant gene action. Significant digenic epistatic interactions were detected for one pair of these QTL. Only a few QTL for S. reiliana were in common with QTL for resistance to Ustilago maydis and Puccinia sorghi, identified at a German location for the same population. Consequently, in our materials resistance to these three fungal pathogens of maize seems to be inherited independently.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051273
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  • 32
    Digitale Medien
    Digitale Medien
    Linkage relationships among molecular markers and storage root traits of carrot (Daucus carota L. ssp. sativus) (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 58-64 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Genetic map ; RFLP ; AFLP ; RAPD ; SAMPL ; Daucus carota L. ssp. sativus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A 109-point linkage map consisting of three phenotypic loci (P 1, Y 2, and Rs), six restriction fragment length polymorphisms (RFLPs), two random amplified polymorphic DNAs (RAPDs), 96 amplified fragment length polymorphisms (AFLPs), and two selective amplification of microsatellite polymorphic loci (SAMPL) was constructed for carrot (Daucus carota L. ssp. sativus; 2n=2x=18). The incidence of polymorphism was 36% for RFLP probes, 20% for RAPD primers, and 42% for AFLP primers. The overall incidence of disturbed segregation was 18%. Linkage relationships at a LOD score of 4.0 and θ=0.25 indicated 11 linkage groups. The total map length was 534.4 cM and the map was clearly unsaturated with markers spaced at 4.9 cM. AFLP P6B15 was 1.7 cM from P 1, AFLP P1B34 was 2.2 cM from Y 2, and AFLP P3B30XA was 8.1 cM from Rs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051208
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  • 33
    Digitale Medien
    Digitale Medien
    Novel polymorphisms in the upstream region of the human dopamine D4 receptor (DRD4) gene (1999)
    Springer
    Journal of human genetics 44 (1999), S. 416-418 
    Details
    ISSN: 1435-232X
    Schlagwort(e): Key words Dopamine D4 receptor gene ; Promoter ; Polymorphisms ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract We found nine novel polymorphisms in the upstream region of the human dopamine D4 receptor (DRD4) gene of Japanese by direct sequencing. These polymorphisms are −809G 〉 A, −768G 〉 A, −616C 〉 G, −603T 〉 del, −602G 〉 del, −600G 〉 C, −376C 〉 T, −291C 〉 T, and −128G 〉 T. One known polymorphism, −521C 〉 T, was also recognized. Six of these sites were identified as restriction fragment length polymorphisms (RFLPs).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s100380050191
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  • 34
    Digitale Medien
    Digitale Medien
    Mapping loci associated with milling yield in wheat (Triticum aestivum L.) (1999)
    Springer
    Molecular breeding 5 (1999), S. 561-568 
    Details
    ISSN: 1572-9788
    Schlagwort(e): wheat ; milling yield ; QTL mapping ; RFLP ; microsatellite
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A partial genetic linkage map constructed using 150 single seed descent (SSD) lines generated from a cross between the hexaploid wheat varieties ‘Schomburgk’ and ‘Yarralinka’ was used to identify loci controlling milling yield. Milling yield data were obtained using seed collected from field trials conducted at different sites over two seasons. The estimated broad-sense heritability of milling yield in this population was calculated as 0.48. In the preliminary analysis, two regions were identified on chromosomes 3A and 7D, which were significantly associated with milling yield and accounted for 22% and 19% of the genetic variation, respectively. Bulked segregant analysis in combination with AFLP identified other markers linked to these loci, as well as an additional region on chromosome 5A, which accounted for 19% of the genetic variation. The applicability of these markers as selection tools for breeding purposes is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009678023431
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  • 35
    Digitale Medien
    Digitale Medien
    Differentiating PVYNTN by unique single-restriction cleavage of PCR products (1999)
    Springer
    Potato research 42 (1999), S. 215-221 
    Details
    ISSN: 1871-4528
    Schlagwort(e): potato virus Y ; strain differentiation ; RFLP ; potato tuber necrotic ringspot disease ; Solanum tuberosum L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary A procedure for differentiating PVYNTN from PVYN is described and is based on the unique cleavage of their respective PCR products with strain specific restriction endonucleases. The PCR products corresponding to the 5′ end of the N and NTN strains of PVY were cloned and sequenced, and a restriction map was constructed which included common enzymes that were used for the differentiation of PVYNTN. Unique, single cleavage of PCR products derived from the 5′ end of the PVYNTN genome by Nco I, and that of the N-strain of PVY by Bgl II restriction endonuclease were demonstrated. The specific digestion patterns in polyacrylamide gel were used for the unequivocal differentiation between the N and NTN strains of the virus. Both single and mixed infections were detected in field samples of potatoes using this procedure.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02358411
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  • 36
    Digitale Medien
    Digitale Medien
    Genetic and physical characterization of a region of Arabidopsis chromosome 1 containing the CLAVATA1 gene (1999)
    Springer
    Plant molecular biology 39 (1999), S. 171-176 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Arabidopsis ; CLAVATA1 ; genome sequencing ; RFLP ; YAC contig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract With the advance of Arabidopsis as a model system for understanding plant genetics, development and biochemistry, a detailed description of the genome is necessary. As such, focused projects are underway to map and sequence the Arabidopsis nuclear genome. We have characterized a region of chromosome 1, surrounding the CLAVATA1 (CLV1) locus. Three (RFLP) clones were mapped relative to clv1-1, and were used to construct an ca. 700 kb yeast artificial chromosome (YAC) contig. Three cosmids spanning the CLV1 locus were analyzed and ca. 24 kb of genomic DNA was sequenced, including a continuous stretch of 18 kb. In addition to generating clones in this region of chromosome 1, we have analyzed the size, spacing and organization of several contiguous genes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006127302671
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  • 37
    Digitale Medien
    Digitale Medien
    Identification of quantitative trait loci for nitrogen use efficiency in maize (1999)
    Springer
    Molecular breeding 5 (1999), S. 187-195 
    Details
    ISSN: 1572-9788
    Schlagwort(e): gene mapping ; low nitrogen tolerance ; nitrogen use efficiency ; RFLP ; Zea mays L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Intensively managed crop systems are normally dependent on nitrogen input to maximize yield potential. Improvements in nitrogen- use efficiency (NUE) in crop plants may support the development of cropping systems that are more economically efficient and environment friendly. The objective of this study was to map and characterize quantitative trait loci (QTL) for NUE in a maize population. In preliminary experiments, inbred lines contrasting for NUE were identified and were used to generate populations of F2:3 families for genetic study. A total of 214 F2:3 families were evaluated in replicated trials under high nitrogen (280 kg/ha) and low nitrogen (30 kg/ha) conditions in 1996 and 1997. Analysis of ear-leaf area, plant height, grain yield, ears per plant, kernels number per ear, and kernel weight indicated significant genetic variation among F2:3 families. The heritability of these traits was found to be high (h2=0.57–0.81). The mapping population were genotyped using a set of 99 restriction fragment length polymorphism (RFLP) markers. A linkage map of these markers was developed and used to identify QTL. Between two and six loci were found to be associated with each trait. The correspondence of several genomic regions with traits measured under nitrogen limited conditions suggests the presence of QTL associated with NUE. QTLs will help breeders to improve their maize ideotype of a low-nitrogen efficiency by identifying those constitutive and adaptive traits involved in the expression of traits significantly correlated with yield, such as ear leaf area and number of ears per plant.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009669507144
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  • 38
    Digitale Medien
    Digitale Medien
    Ultrastructural and molecular study of plastid inheritance in Abies alba and some Abies hybrids (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 284-291 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Key words Abies ; Egg cell ; Plastid inheritance ; RFLP ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The ultrastructure of egg cells in Abies alba was examined to elucidate the lack of maternal inheritance of plastids. Before fertilization, maternal plastids are absent in the perinuclar zone containing mainly mitochondria and smooth endoplasmic reticulum. During egg cell development the maternal plastids are transformed into large inclusions which are situated mostly towards the periphery of the egg cell, and finally disintegrate. As a consequence, they do not participate in zygote formation. RFLP analysis of cpDNA of parental trees and their F1 interspecific hybrids (A. alba×A. numidica, A. alba×A. nordmanniana, A. nordmanniana×A. Alba) using HindIII and BamHI showed a paternal mode of cpDNA inheritance. Paternal inheritance has also been found with PCR/RFLP analysis of cpDNA from parental trees and their hybrids (A. alba×A. pinsapo, A. pinsapo×A. alba, A. pinsapo×A. numidica) using ApaI and HaeIII digests, as well as in the crosses of A. cephalonica×A. nordmanniana, A. nordmanniana×A. cephalonica, A. cephalonica×A. numidica using TagI digests.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004970050155
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  • 39
    Digitale Medien
    Digitale Medien
    Construction of RFLP-based maps of foxtail millet, Setaria italica (L.) P. Beauv. (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 31-36 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Foxtail millet ; Genetic map ; Primary trisomics ; RFLP ; Setaria italica ; Setaria viridis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  An RFLP-based map consisting of 160 loci was constructed in an intervarietal cross of foxtail millet [Setaria italica (L.) P. Beauv.], Longgu 25×Pagoda Flower Green. The map comprises nine linkage groups, which were aligned with the nine foxtail millet chromosomes using trisomic lines, and spans 964 cM. The intraspecific map was compared to an interspecific map, constructed in a S. italica×S. viridis cross. Both the order of the markers and the genetic distances between the loci were highly conserved. Deviations from the expected 1 : 2 : 1 Mendelian segregation ratios were observed in both the intra- and inter-specific populations. The segregation data indicate that chromosome VIII in the Longgu 25×Pagoda Flower Green cross carries a gene that strongly affects gamete fertility.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050705
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  • 40
    Digitale Medien
    Digitale Medien
    Organization of an Arabidopsis thaliana gene cluster on chromosome 4 including the RPS2 gene, in the Brassica nigra genome (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 468-474 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Microsynteny ; Genome ; Gene cluster ; RFLP ; PFGE
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Genetic and physical maps, consisting of a large number of DNA markers for Arabidopsis thaliana chromosomes, represent excellent tools to determine the organization of related genomes such as those of Brassica. In this paper we report the chromosomal localization and physical analysis by pulsed-field gel electrophoresis (PFGE) of a well-defined gene complex of A. thaliana in the Brassica nigra genome (B genome n=8). This complex is approximately 30 kb in length in A. thaliana and contains a cluster of six genes including ABI1 (ABA-responsive), RPS2 (resistance against Pseudomonas syringae, a bacterial disease), CK1 (casein kinase I), NAP (nucleosome-assembly protein), X9 and X14 (both of unknown function). The Arabidopsis chromosomal complex was found to be duplicated and conserved in gene number at different levels in the Brassica genome. Linkage group B1 had the most-conserved arrangement carrying all six genes tightly linked. Group B4 had an almost complete complex except for the absence of RPS2. Other partial complexes of fewer members were found on three other chromosomes. Our studies demonstrate that by this approach it is possible to identify ancestrally related chromosome segments in a complex and duplicated genome, such as the genome of B. nigra, permitting one to draw conclusions as to its origin and evolution.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050763
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  • 41
    Digitale Medien
    Digitale Medien
    Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 812-822 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Citrus ; RFLP ; RAPD ; Phylogeny ; Taxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Relationships among 88 accessions representing 45 Citrus species, three man-made hybrids, and six related genera were examined for restriction fragment length polymorphisms (RFLP). Thirty-two Citrus and three Microcitrus accessions were also examined by random amplified polymorphic DNA (RAPD) analysis. A measure of relative heterozygosity was estimated based on the mean of the number of fragments per individual per probe-enzyme combination (PEC) divided by total number of fragments per PEC for all non-hybrid Citrus individuals. The presence in a Citrus species of a rare band found also in a related genus was taken as an indication of possible introgression, while the presence of several fragments unique to 1 species was used to indicate non-involvement of that species in hybridization events. Most species that have been described in the literature as hybrids had high heterozygosity indices and no unique fragments. Distance matrices and dendrograms were generated using simple matching coefficient and neighbor-joining cluster analysis. RFLP and RAPD data gave approximately the same results. These data showed C. maxima was affiliated with the papedas C. hongheensis and C. latipes. C. medica clustered with C. indica when only non-hybrid taxa were examined, or among limes, lemons, and relatives when all species were considered. Mandarins did not show strongly supported groupings among themselves, nor with other species. These data showed that several accessions were probably assigned to the wrong species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050807
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  • 42
    Digitale Medien
    Digitale Medien
    Mapping of the gene Nxphu that controls hypersensitive resistance to potato virus X in Solanum phureja IvP35 (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 840-843 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Potato virus X ; Resistance gene ; Genetic mapping ; RFLP ; Solanaceae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The line IvP35 of the diploid (2n=2x=24) cultivated potato species Solanum phureja (family Solanaceae) expresses hypersensitive resistance (H) to potato X potexvirus (PVX). In this study, a diploid potato population was produced using IvP35 as the male parent and a diploid line of S. tuberosum (87HW13.7) as the female parent and tested for resistance to PVX. Data indicated that H to PVX in IvP35 is a dominant, monogenically inherited trait controlled by a single gene, named Nx phu , that is in a simplex condition (Nxnx). RFLP analysis carried out on the progeny lines revealed 4 markers (CT220, TG328, CT112 and TG424) from the long arm of chromosome IX that were linked to the hypersensitive phenotype; the closest linkage was observed with the marker TG424. Previous authors have shown that the same region of chromosome IX contains the gene Sw-5 for resistance to tomato spotted wilt tospovirus in Lycopersicon peruvianum (Solanaceae).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050810
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  • 43
    Digitale Medien
    Digitale Medien
    RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 719-726 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Tomato and Solanum lycopersicoides intergeneric hybrid ; Chloroplast DNA ; Nuclear genome ; RFLP ; GISH
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  RFLP (restriction fragment length polymorphism) and GISH (genomic in situ hybridization) analyses were employed to identify the chloroplast and nuclear genomes of the somatic hybrids and progeny between tomato ‘Ohgata zuiko’ and Solanum lycopersicoides (‘LA 2386’). A random distribution of the chloroplast genotype was determined using a cloned 19.6-kb BamHI fragment (Ba1) of tobacco chloroplast DNA. Eight selected hybrids were analyzed for their chromosomal compositions; 4 were tetraploids (2n=48) with an equal number of chromosomes derived from each parent as accurately determined by GISH, and the other 4 were hexaploids, containing an average of two sets of tomato chromosomes and one set from the wild parent. RFLP analysis with six tomato nuclear probes of known chromosomal locations revealed no major variation among the 44 hybrid plants surveyed. However, it also showed the presence of both parent-specific alleles and the loss of some and the presence of a few non-parental alleles, indicating rearrangement and/or recombination of the nuclear DNA. The relevance of the molecular and cytological methods and the potential use of somatic hybrids for plant breeding are demonstrated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050794
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  • 44
    Digitale Medien
    Digitale Medien
    Genetic basis of resistance to sugarcane mosaic virus in European maize germplasm (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1151-1161 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words European maize ; Zea mays ; Sugarcane mosaic virus ; Disease resistance ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Sugarcane mosaic virus (SCMV) causes considerable damage to maize (Zea mays L.) in Europe. The objective of the present study was to determine the genetic basis of resistance to SCMV in European maize germplasm and to compare it with that of U.S. inbred Pa405. Three resistant European inbreds D21, D32, and FAP1360A were crossed with four susceptible inbreds F7, KW1292, D408, and D145 to produce four F2 populations and three backcrosses to the susceptible parent. Screening for SCMV resistance in parental inbreds and segregating generations was done in two field trials as well as under greenhouse conditions. RFLP markers umc85, bnl6.29, umc10, umc44, and SSR marker phi075 were used in F2 populations or F3 lines to locate the resistance gene(s) in the maize genome. Segregation in the F2 and backcross generations fitted to different gene models depending on the environmental conditions and the genotype of the susceptible parent. In the field tests, resistance in the three resistant European inbreds seems to be controlled by two to three genes. Under greenhouse conditions, susceptibility to SCMV in D32 appears to be governed by one dominant and one recessive gene. Allelism tests indicated the presence of a common dominant gene (denoted as Scm1) in all three resistant European inbreds and Pa405. Marker analyses mapped two dominant genes: Scm1 on chromosome 6S and Scm2 on chromosome 3.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050851
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  • 45
    Digitale Medien
    Digitale Medien
    Characterisation of the radish introgression carrying the Rfo restorer gene for the Ogu-INRA cytoplasmic male sterility in rapeseed (Brassica napus L.) (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 129-134 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Brassica napus ; Raphanus sativus ; Restorer gene ; Introgression ; RFLP ; RAPD ; Genetic mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Bulked segregant analysis and comparative mapping were applied to identify molecular markers linked to the Rfo restorer gene used for the Ogu-INRA cytoplasmic male-sterility system in rapeseed. These markers were then used to localise the radish introgression on the B. napus genetic map constructed from the cross ‘Darmor.bzh’ x ’Yudal’. The introgression mapped on the DY15 linkage group. From the comparison of this latter group to the linkage group constructed on a F2 progeny segregating for the radish introgression, it was concluded that the introgression had occurred through homoeologous recombination, that it was not distal and that it had replaced a B. napus region of around 50 cM. A QTL involved in aliphatic seed glucosinolate content was located on the DY15 linkage group at a position corresponding to one end of the introgression. The DNA markers identified in this study are being used in map-based cloning of the Rfo gene and in marker-assisted selection.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050876
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  • 46
    Digitale Medien
    Digitale Medien
    Mapping of three self-fertility mutations in rye (Secale cereale L.) using RFLP, isozyme and morphological markers (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 147-153 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Genetic mapping ; Isozyme ; RFLP ; Secale cereale L. ; Self-fertility
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Three mutations determining self-fertility at the S, Z and S5 self-incompatibility loci on chromosomes 1R, 2R and 5R of rye, respectively, were mapped using three different F2 populations. There was a close linkage of one isozyme and four RFLP markers, and no recombinant plants were detected. These markers are Prx7, Xiag249 and Xpsr634 for the S locus (1R), Xbcd266 for the Z locus (2R) and Xpsr100 for the S5 locus (5R). Linkage data for markers associated to the self-fertility mutations at the S, Z and S5 loci were calculated and compared with genetic maps computed by MAPMAKER multipoint analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050879
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  • 47
    Digitale Medien
    Digitale Medien
    Codominant PCR-based markers for Pinus taeda developed from mapped cDNA clones (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 327-336 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words STS ; Codominant PCR marker ; RFLP ; Loblolly pine (Pinus taeda L.) ; Comparative map
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  We report a strategy for developing codominant PCR-based genetic markers by using sequenced cDNA clones from loblolly pine (Pinus taeda L.). These clones were previously used as probes for detecting restriction fragment length polymorphisms (RFLPs) to generate linkage maps. After assessing the complexity of banding patterns from Southern blots, we selected clones representing relatively simple gene families, and then determined nucleotide sequences for about 200 bp at each end of the cDNA inserts. Specific PCR primers were designed to amplify samples of genomic DNA derived from two loblolly pine mapping populations. Polymorphisms were detected after digesting the amplified DNA fragments with a battery of restriction endonucleases, and most polymorphisms were inherited in a Mendelian fashion. These newly identified genetic markers are codominant and relatively simple to use. By assaying DNA from individuals used to construct RFLP maps, we show that most of these markers map to the same position as the RFLP loci detected using their corresponding cDNAs as probes, implying that these markers have been converted from RFLP to PCR-based methods. These PCR-based markers will be useful for genome mapping and population genetics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050903
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  • 48
    Digitale Medien
    Digitale Medien
    Comparative QTL mapping of resistance to Ustilago maydis across four populations of European flint-maize (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1321-1330 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Maize ; Ustilago maydis ; QTL mapping ; RFLP ; Resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  We mapped and characterized quantitative trait loci (QTLs) for resistance to Ustilago maydis and investigated their consistency across different flint-maize populations. Four independent populations, comprising 280 F3 lines (A×BI), 120 F5 lines (A×BII), 131 F4 lines (A×C) and 133 F4 lines (C×D), were produced from four European elite flint inbreds (A, B, C, D) and genotyped at 89, 151, 104, and 122 RFLP marker loci, respectively. All Fn lines were evaluated in field trials with two replications in five German environments. Genotypic variances were highly significant for the percentage of U. maydis infected plants (UST) in all populations, and heritabilities exceeded 0.69. Between five and ten QTLs were detected in individual populations by composite interval mapping, explaining between 39% and 58% of the phenotypic variance. These 19 different QTLs were distributed over all ten chromosomes without any clustering on certain chromosomes. In most cases, gene action was dominant or overdominant. Fourteen pairs of the detected QTLs for UST displayed significant digenic epistatic interactions, but only two of them did so after arcsin √UST/100 transformation. Significant QTL× environment interactions occurred frequently. Between two to four QTLs were common between pairs of populations. Population C×D was also grown in Chartres, a location with a high U. maydis incidence. Two out of six QTLs identified for Chartres were in common with QTLs detected across five German environments for C×D. Consequently, marker-assisted or phenotypic selection based on results from natural infection seem to be suitable breeding strategies for improving the resistance of maize to U. maydis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051025
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  • 49
    Digitale Medien
    Digitale Medien
    Prediction of testcross means and variances among F3 progenies of F1 crosses from testcross means and genetic distances of their parents in maize (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 503-512 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Zea mays L ; AFLP ; RFLP ; Testcross mean ; Testcross variance ; Genetic distance ; Midparent heterosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Prediction of the means and genetic variances in segregating generations could help to assess the breeding potential of base populations. In this study, we investigated whether the testcross (TC) means and variances of F3 progenies from F1 crosses in European maize can be predicted from the TC means of their parents and F1 crosses and four measures of parental genetic divergence: genetic distance (GD) determined by 194 RFLP or 691 AFLPTM 1 markers, mid-parent heterosis (MPH), and absolute difference between the TC means of parents (∣P1−P2∣). The experimental materials comprised six sets of crosses; each set consisted of four elite inbreds from the flint or dent germplasm and the six possible F1 crosses between them, which were evaluated for mid-parent heterosis. Testcross progenies of these materials and 20 random F3 plants per F1 cross were produced with a single-cross tester from the opposite heterotic group and evaluated in two environments. The characters studied were plant height, dry matter content and grain yield. The genetic distance between parent lines ranged between 0.17 and 0.70 for RFLPs and between 0.14 and 0.57 for AFLPs in the six sets. Testcross-means of parents, F1 crosses, and F3 populations averaged across the six crosses in a particular set generally agreed well for all three traits. Bartlett’s test revealed heterogeneous TC variances among the six crosses in all sets for plant height, in four sets for grain yield and in five sets for dry matter content. Correlations among the TC means of the parents, F1 crosses, and F3 populations were highly significant and positive for all traits. Estimates of the TC variance among F3 progenies for the 36 crosses showed only low correlations with the four measures of parental genetic divergence for all traits. The results demonstrated that for our material, the TC means of the parents or the parental F1 cross can be used as predictors for the TC means of F3 populations. However, the prediction of the TC variance remains an unsolved problem.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050767
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  • 50
    Digitale Medien
    Digitale Medien
    Molecular phylogeny of mangroves V. Analysis of genome relationships in mangrove species using RAPD and RFLP markers (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 617-625 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Mangroves ; Genome relationship ; Phylogeny ; RAPD ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  DNA from pooled leaf samples of 11 true major mangrove, three true minor mangrove, two mangrove associate, two mangrove parasite, three terrestrial and one cultivated species were isolated for the present study. In total, 198 random amplified polymorphic DNAs (RAPDs) and 180 restriction fragment length polymorphism (RFLP) loci were scored by using ten primers and 14 enzyme-probe combinations respectively. The polymorphism observed for these markers revealed a high degree of genetic diversity in mangroves at both inter-specific or inter-generic levels. A dendrogram, constructed after pooling both RAPD and RFLP data, using a similarity index was analysed for genome relationships among these species. The dendrogram showed clustering of all the major mangroves, except for Nypa fruticans (Arecaceae), into one group. All species under the tribe Rhizophorae formed a sub-cluster, to which Xylocarpus granatum was found to be the most closesly related species. The clustering pattern implied that Excoecaria agallocha and Acanthus ilicifolius should be considered as true minor mangroves. The present study also provided molecular data favouring the separation of Avicennia spp. from the Verbenaceae to create a monotypic family the Avicenniaceae. The separation of Viscum orientale into the Viscaceae was also favoured.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050937
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  • 51
    Digitale Medien
    Digitale Medien
    Molecular mapping of the photoperiod response gene ea7 in barley (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 797-800 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Genetic mapping ; RFLP ; Flowering time ; Photoperiod response ; Barley
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The gene ea 7 determining photoperiod insensitivity under short day length was mapped on the short arm of chromosome 6H near the centromere. The gene was linked to the two flanking markers Xmwg2264 and Xmwg916 by 6.7 and 13.0 cM, respectively. Compared to Ppd-H1 (chromosome 2H) and Ppd-H2 (chromosome 1H), ea 7 determines the strongest effect on flowering time with 55 and 18 days difference compared to photoperiod sensitive genotypes grown under short and long photoperiods, respectively. Allelic and homoeologous relationships to major genes and quantitative trait loci controlling flowering time in barley and wheat are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050958
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  • 52
    Digitale Medien
    Digitale Medien
    Molecular mapping of the py-1 gene for resistance to corky root rot (Pyrenochaeta lycopersici) in tomato (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 784-788 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words CAP ; py-1 ; RAPD ; RFLP ; Breeding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  We report the molecular mapping of the py-1 gene for resistance to corky root rot [Pyrenochaeta lycopersici (Schneider and Gerlach)] in tomato using RAPD and RFLP marker analysis. DNA from near-isogenic lines (NILs) of tomato differing in corky root rot resistance was screened with 575 random oligonucleotide primers to detect polymorphic DNAs linked to py-1. Three primers (OPW-04, OPC-02, OPG-19) revealed polymorphisms between the NILs. Twelve resistant and eight susceptible DNA pools derived from segregating F3 families were used to confirm that the RAPD markers were linked to the py-1 gene. Two of the linked amplified fragments, corresponding to OPW-04 and OPC-02, were subsequently cloned and mapped on the tomato molecular linkage map as RFLPs. These clones were located between TG40 and CT31 on the short arm of chromosome 3. Further analysis with selected RFLP markers showed that 7% (8.8 cM) of chromosome 3 of the resistant line ‘Moboglan’ was introgressed from the L. peruvianum donor parent. Three RFLP markers (TG40, TG324, and TG479) from the introgressed part of chromosome 3 were converted to cleaved amplified polymorphism (CAP) markers for use in a polymerase chain reaction (PCR) assay. These PCR markers will allow rapid large-scale screening of tomato populations for corky root rot resistance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050956
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  • 53
    Digitale Medien
    Digitale Medien
    Identification and physical mapping of three Haynaldia villosa chromosome-6V deletion lines (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1042-1046 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words T. aestivum ; H. villosa ; C-banding ; RFLP ; Deletion mapping ; Pm21
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Three deletion lines (del6V?2S-1, del6V? 2L-1, and del6V?2L-2) of Haynaldia villosa chromosome 6V added to wheat were identified by C-banding and characterized by RFLP analyses. The breakpoints were located at fraction lengths (FL) 0.58 in del6V?2S-1 in the short arm, and FL 0.66 in del6V?2L-1 and FL 0.64 in del6V?2L-2 in the long arm. Thirty-one Triticeae homoeologous group-6 DNA probes were used to map RFLP loci in the deletion lines and the wheat-H. villosa disomic substitution (DS) line 6V?2(6A). Nine probes failed to detect polymorphism between Chinese Spring and DS6V?2(6A). Ten of sixteen polymorphic short-arm loci were not detected in del6V?2S-1. Thus, the loci are located in the deleted distal chromosome region. Six RFLP markers were mapped in the proximal 58% of 6VS. Of 20 DNA markers specific for 6VL, six mapped in the distal 36% of the long arm, and nine mapped in the proximal 64% of 6VL. The breakpoint of the short arm of 6V?2 occurs between Xpsr106 and Xcdo270, and that of the long arm between Xpsr915 and Xmwg934. The powdery mildew resistance gene Pm21 is located on the short arm of chromosome 6V?2. Pm21 is present in del6V?2S-1, and can be further mapped in the proximal 58% of 6V?2S.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050989
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  • 54
    Digitale Medien
    Digitale Medien
    The structure of the Aegilops tauschii genepool and the evolution of hexaploid wheat (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 657-670 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Triticum aestivum ; Phylogeny ; Genetic distance ; Genome ; Introgression ; Allopolyploidy ; RFLP ; Glutenin ; rRNA ; Non-transcribed spacers ; Evolution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Polymorphism in the lengths of restriction fragments at 53 single-copy loci, the rRNA locus Nor3, and the high-molecular-weight glutenin locus Glu1 was investigated in the D genome of hexaploid Triticum aestivum and that of Aegilops tauschii, the source of the T. aestivum D genome. The distribution of genetic variation in Ae. tauschii suggests gene flow between Ae. tauschii ssp. strangulata and ssp. tauschii in Iran but less in Transcaucasia. The “strangulata” genepool is wider than it appears on the basis of morphology and includes ssp. strangulata in Transcaucasia and southeastern (SE) Caspian Iran and ssp. tauschii in north-central Iran and southwestern (SW) Caspian Iran. In the latter region, Ae. tauschii morphological varieties ‘meyeri’ and ‘typica’ are equidistant to ssp. strangulata in Transcaucasia, and both belong to the “strangulata” genepool. A model of the evolution of Ae. tauschii is presented. On the geographic region basis, the D genomes of all investigated forms of T. aestivum are most closely related to the “strangulata” genepool in Transcaucasia, Armenia in particular, and SW Caspian Iran. It is suggested that the principal area of the origin of T. aestivum is Armenia, but the SW coastal area of the Caspian Sea and a corridor between the two areas may have played a role as well. Little genetic differentiation was found among the D genomes of all investigated free-threshing and hulled forms of T. aestivum, and all appear to share a single D-genome genepool, in spite of the fact that several Ae. tauschii parents were involved in the evolution of T. aestivum.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050942
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  • 55
    Digitale Medien
    Digitale Medien
    A genetic linkage map of durum wheat (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 721-728 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Durum wheat ; Mapping ; Linkage map ; RFLP ; Morphological markers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A genetic linkage map of tetraploid wheat [Triticum turgidum (L.) Thell.] was constructed using segregation data from a population of 65 recombinant inbred lines (RILs) derived from a cross between the durum wheat cultivar Messapia and accession MG4343 of T. turgidum (L.) Thell. ssp dicoccoides (Korn.) Thell. A total of 259 loci were analysed, including 244 restriction fragment length polymorphisms (RFLPs), one PCR (polymerase chain reaction) marker (a sequence coding for a LMW (low-molecular-weight) glutenin subunit gene located at the Glu-B3 locus), seven biochemical (six seed-storage protein loci and one isozyme locus) and seven morphological markers. A total of 213 loci were mapped at a LOD≥3 on all 14 chromosomes of the A and B genomes. The total length of the map is 1352 cM and the average distance between adjacent markers is 6.3 cM. Forty six loci could not be mapped at a LOD≥3. A fraction (18.6%) of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were found on chromosomes 1B, 3AL, 4AL, 6AL and 7AL. The durum wheat map was compared with the published maps of bread wheat using several common RFLP markers and general features are discussed. The markers detected the known structural rearrangements involving chromosomes 4A, 5A and 7B as well as the translocation between 2B-6B, but not the deletion on 2BS. This map provides a useful tool for analysing and breeding economically important quantitative traits and for marker-assisted selection, as well as for studies of genome organisation in small grain cereal species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050948
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  • 56
    Digitale Medien
    Digitale Medien
    QTLs conditioning early growth in a soybean population segregating for growth habit (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1210-1216 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Soybean ; Glycine max ; RFLP ; QTL ; Plant height ; Canopy width
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  There are both economic and environmental reasons for reducing the use of herbicides for weed control in soybean [Glycine max (L.) Merr.] fields. Optimizing crop competitiveness can reduce reliance on chemical weed control. Fast and vigorous early growth and rapid canopy development can be effective in suppressing weed infestation of crop plants. The purposes of this study were to identify and molecularly map the quantitative trait loci (QTLs) conditioning soybean plant height and canopy width during the early vegetative stages of soybean growth. A restriction fragment length polymorphism (RFLP) linkage map was created using 142 markers and 116 F2-derived lines from a cross of ‘S100’בTokyo’. The parents and the 116 F2-derived lines were evaluated in the greenhouse and in the field at Athens, Ga., in 1996 and 1997. Combined over environments, Tokyo averaged 41 and 17% taller plants than S100 at the V7 and V10 stages of development. Transgressive segregation was observed among the progeny at both stages. Based on single-factor analysis of variance (ANOVA), three and four independent RFLP loci were associated with plant height at the V7 and V10 stages, respectively. All three loci detected [on linkage groups (LGs) C2 and F, and unlinked] at the V7 stage were also detected at the V10 stage along with one additional independent locus on LG E. The Tokyo allele contributed to increased plant height at all loci except at the unlinked locus. Three QTLs (on LGs C2, E, and F) were consistent across environments, three (on LGs C2 and F, and unlinked) were consistent across stages of plant development, and two (on LGs C2 and F) were consistent both across environments and stages of plant development. Within each stage of development, there was no interaction among the independent loci, and the respective loci together explained most of the variation in the traits. Three independent RFLP loci were associated with canopy width at the V10 stage, of which one was unique to the trait, while the remaining loci (on LGs C2 and F) were in common with the independent loci for plant height. Canopy width had a strong correlation (r=0.87) with plant height at the V10 stage. However, mature plant height, lodging, or seed weight had no phenotypic or QTL association with early plant height or canopy width.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051012
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  • 57
    Digitale Medien
    Digitale Medien
    A consensus linkage map of rye (Secale cereale L.) including 374 RFLPs, 24 isozymes and 15 gene loci (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1279-1288 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Consensus map ; Isozymes ; RFLP ; Rye ; Secale cereale L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Consensus linkage maps were constructed for all seven rye chromosomes using 12 basic RFLP maps. The maps presented contain a total of 413 markers. The number of markers per chromosome varies from 41 (chromosome 3R) to 83 (chromosome 1R). In addition to 374 RFLP and 24 isozyme markers 15 gene loci were incorporated, determining the traits reduced plant height, self fertility, male sterility restoration, vernalization response, resistance against powdery mildew, chlorophyll deficiency, hairy leaf sheath, hairy peduncle, waxy endosperm, waxless plant and absence of ligules. The maps presented allow the selection of markers for the fine mapping of certain regions of the rye genome. In terms of the known chromosomal rearrangements within the Triticeae its utilization can also be extended for mapping in wheat and barley.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051020
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  • 58
    Digitale Medien
    Digitale Medien
    Identification of molecular markers linked to quantitative trait loci for soybean resistance to corn earworm (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 786-790 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Soybean ; Glycine max ; QTL ; RFLP ; Antixenosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  One hundred and thirty nine restriction fragment length polymorphisms (RFLPs) were used to construct a soybean (Glycine max L. Merr.) genetic linkage map and to identify quantitative trait loci (QTLs) associated with resistance to corn earworm (Helicoverpa zea Boddie) in a population of 103 F2-derived lines from a cross of ‘Cobb’ (susceptible) and PI229358 (resistant). The genetic linkage map consisted of 128 markers which converged onto 30 linkage groups covering approximately 1325 cM. There were 11 unlinked markers. The F2-derived lines and the two parents were grown in the field under a plastic mesh cage near Athens, Ga., in 1995. The plants were artificially infested with corn earworm and evaluated for the amount of defoliation. Using interval-mapping analysis for linked markers and single-factor analysis of variance (ANOVA), markers were tested for an association with resistance. One major and two minor QTLs for resistance were identified in this population. The PI229358 allele contributed insect resistance at all three QTLs. The major QTL is linked to the RFLP marker A584 on linkage group (LG) ‘M’ of the USDA/Iowa State University public soybean genetic map. It accounts for 37% of the total variation for resistance in this cross. The minor QTLs are linked to the RFLP markers R249 (LG ‘H’) and Bng047 (LG ‘D1’). These markers explain 16% and 10% of variation, respectively. The heritability (h2) for resistance was estimated as 64% in this population.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050803
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  • 59
    Digitale Medien
    Digitale Medien
    Structural rearrangement in chromosome 2M of Aegilops comosa has prevented the utilization of the Compair and related wheat-Ae. comosa translocations in wheat improvement (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 780-785 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Wheat ; Aegilops comosa ; Translocation ; Homoeologous recombination ; RFLP ; C-banding ; Genomic in situ hybridization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The genetic constitutions of chromosome 2M of Aegilops comosa and the derived wheat-Ae. comosa translocations were analyzed by molecular cytogenetic techniques. Hybridization of 15 RFLP markers covering the entire length of the group-2 chromosomes revealed that chromosome 2M was structurally rearranged compared to the homoeologous chromosomes of wheat by either a pericentric inversion or a terminal intrachromosomal translocation. The breakpoint of the rearrangement was located in a region between the loci Xpsr131 and Xcdo405, resulting in the translocation of 47% of 2MS to 2ML. This aberrant structure of 2M allowed homoeologous recombination between 2M and its wheat counterpart only in the translocated segment on 2ML. C-banding and genomic in situ hybridization analyses confirmed that all translocation chromosomes consisted of the complete 2MS arm, a large part of 2ML, and very small distal segments derived from 2AS or 2DS, as expected from the aberrant structure of chromosome 2M. Thus, the translocation in the line 2A-2M?4/2 can be described as T2AS-2M?1L ⋅ 2M?1S and the translocations in the lines Compair and 2D-2M?3/8 as T2DS-2M?1L ⋅ 2M?1S. RFLP analysis determined the breakpoints in these translocation chromosomes to be within the telomeric 16% of the wheat chromosome arms. The breakpoint of the 2A/2M translocation was between Xbcd348 and Xcdo783, and that of the 2D/2M translocation was between Xcdo783 and Xpsr666. Because the translocation chromosomes retain the structural aberration found in chromosome 2M, further exploitation of the wheat-Ae. comosa translocations for cultivar improvement is questionable.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050802
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  • 60
    Digitale Medien
    Digitale Medien
    Molecular examination of a chromosome region that controls resistance to potato Y and A potyviruses in potato (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1036-1043 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Potato virus A ; Potato virus Y ; Resistance gene ; RFLP ; Diploid potato
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The gene Ry adg that confers resistance to potato Y potyvirus (PVY) in the cultivated potato [Solanum tuberosum subsp. andigena, line 2x(v-2)7] is located on chromosome XI in a segment that contains three other known resistance genes in other syntenic solanaceous species. One of them is the gene N that controls resistance to tobacco mosaic tobamovirus in tobacco and has previously been isolated and sequenced. Three sequence-related, resistance gene-like (RGL) DNA fragments (354–369 bp) highly homologous to the gene N were PCR-amplified from the potato line 2x(v-2)7. Two RGL fragments (79 and 81% homologous to the N gene) co-segregated with Ry adg among the 77 F1 progeny tested. These RGLs may originate from a resistance gene family on chromosome XI. The potato line 2x(v-2)7 also expressed resistance to potato A potyvirus (PVA), which was controlled by another locus on chromosome XI mapped ca. 6.8 cM distal to Ry adg .
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050836
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  • 61
    Digitale Medien
    Digitale Medien
    Mapping loci associated with flour colour in wheat (Triticum aestivum L.) (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 238-245 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Wheat ; Flour colour ; QTL mapping ; RFLP ; AFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  An RFLP map constructed using 150 single seed descent (SSD) lines from a cross between two hexaploid wheat varieties (‘Schomburgk’בYarralinka’) was used to identify loci controlling flour colour. Flour colour data were obtained from field trials conducted over two seasons at different sites. The estimated heritability of this trait was calculated as 0.67. Two regions identified in the preliminary analysis on chromosomes 3A and 7A, accounted for 13% and 60% of the genetic variation respectively. A detailed analysis of the major locus on 7A was conducted through fine mapping of AFLP markers identified using bulked segregant analysis (BSA). Seven additional markers were identified by the BSA and mapped to the region of the 7A locus. The applicability of these markers to identify wheat lines with enhanced flour colour is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050891
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  • 62
    Digitale Medien
    Digitale Medien
    RFLP mapping of the centromere of chromosome 4 in maize using isochromosomes for 4S (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 361-366 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Centromere ; Telocentric ; Secondary trisomic ; Maize ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The centromere of maize chromosome 4 was previously localized to a 26-cM interval using molecular markers and B-A translocations. The objective of the present study was to refine the placement of the centromere using secondary trisomics. Two independently isolated secondary trisomics (having an isochromosome plus two normal homologs) for 4S were recovered. RFLP analysis of populations segregating for them placed the centromere of chromosome 4 between bnl15.45 and bnl7.20, two RFLP loci that are 5.4-cM apart on the UMC map and 11.5-cM apart on the BNL map.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050749
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  • 63
    Digitale Medien
    Digitale Medien
    RFLP tagging of QTLs conditioning specific leaf weight and leaf size in soybean (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 354-360 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Soybean ; Glycine max ; QTL ; RFLP ; SLW ; Leaf size
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Selection for high specific leaf weight (SLW) in soybean [Glycine max (L) Merr.] may increase apparent photosynthetic rate per unit leaf area (AP), which in turn may improve seed yield. In general, the SLW and leaf size are negatively correlated in soybean. To maximize total photosynthetic performance, and perhaps the seed yield, of a soybean cultivar, it would be necessary to establish a large leaf area rapidly while maintaining a high SLW. The objective of the present study was to identify quantitative trait loci (QTLs) conditioning SLW and leaf size in soybean. One hundred and twenty F4-derived lines from a ‘Young’×PI416937 population were evaluated using restriction fragment length polymorphism (RFLP) markers. The genetic map consisted of 155 loci on 33 linkage groups (LGs) covering 973 cM of map distance. The phenotypic data were collected from two different environments – a greenhouse at Athens, Ga. and a field site at Windblow, N.C. The SLW and leaf-size measurements were made on leaves from the 8th and 9th node of soybean plants at the V12 stage of development. Combined over environments, six putative independent RFLP markers were associated with SLW, and four of these loci were consistent across environments. Individually, the six markers each explained between 8 and 18% of the phenotypic variation among lines for SLW. The Young alleles contributed to a greater SLW at four of the six independent marker loci, and transgressive segregation occurred among the progeny for SLW. Three putative independent RFLP markers were associated with leaf size, each explaining between 6 to 11% of the phenotypic variation in the trait, and one of these markers was identified in both environments. There was no correlation between SLW and leaf size in this population. Similarly, none of the six QTLs conditioning SLW were linked to any of the three QTLs for leaf size. In this soybean population, it is possible to select for progeny lines with greater SLW than either parent perhaps without affecting the leaf size. It is feasible to pyramid all of the desirable alleles for greater SLW and large leaf size in a single genetic background.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050748
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  • 64
    Digitale Medien
    Digitale Medien
    Genetics and molecular mapping of a male fertility restoration locus (Rfg1) in rye (Secale cereale L.) (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 99-102 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Genetic mapping ; CMS ; Fertility restoration ; RAPD ; RFLP ; Rye ; Secale cereale L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A gene determining the restoration of cytoplasmic genic male sterility (CMS) caused by the Gülzow (G)-type cytoplasm was mapped by analyzing an F2 and F3 population comprising 140 and 133 individual plants, respectively. The target gene, designated Rfg1, was mapped on chromosome 4RL distally to three RFLP (Xpsr119, Xpsr167, Xpsr899) and four RAPD (XP01, XAP05, XR11, XS10) loci. Xpsr167 and Xpsr899 are known to be located on the segment of chromosome 4RL which was ancestrally translocated and is homoeologous to the distal end of other Triticeae 6S chromosomes. It is suggested that Rfg1 may be allelic to the gene determining the restoration of rye CMS caused by the Pampa (P) cytoplasm (chromosome 4RL) and to Rfc4 that on rye addition lines of chromosome 4RL restores male fertility of hexaploid wheat with T. timopheevi cytoplasm. Homoeoallelism to two loci for cytoplasmic-male-sterility restoration on chromosomes 6AS and 6BS in hexaploid wheat is also suggested.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050871
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  • 65
    Digitale Medien
    Digitale Medien
    Genetic linkage map of peach [Prunus persica (L.) Batsch] using morphological and molecular markers (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 888-895 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Prunus persica ; Linkage map ; RFLP ; RAPD ; AFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A genetic linkage map of peach [Prunus persica (L.) Batch] was constructed in order to identify molecular markers linked to economically important agronomic traits that would be particularly useful for long-lived perennial species. An intraspecific F2 population was generated from self-pollinating a single F1 plant from a cross between a flat non-acid peach, ‘Ferjalou Jalousia®’ and an acid round nectarine ‘Fantasia’. Mendelian segregations were observed for 270 markers including four agronomic characters (peach/nectarine, flat/round fruit, acid/non-acid fruit, and pollen sterility) and 1 isoenzyme, 50 RFLP, 92 RAPD, 8 inter-microsatellite amplification (IMA), and 115 amplified fragment length polymorphism (AFLP) markers. Two hundred and forty-nine markers were mapped to 11 linkage groups covering 712 centiMorgans (cM). The average density between pairs of markers is 4.5 cM. For the four agronomic characters studied, molecular markers were identified. This map will be used for the detection of QTL controlling fruit quality in peach and, particularly, the acid and sugar content.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050969
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  • 66
    Digitale Medien
    Digitale Medien
    Comparative RFLP mapping of Triticum monococcum genes controlling vernalization requirement (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 968-975 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Triticum monococcum ; Vernalization genes ; Vrn-1 ; Vrn-2 ; RFLP ; Comparative maps
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The adaptability of Triticum aestivum to a large range of environments is partially due to genetic differences in sensitivity to vernalization. The most potent gene reducing the vernalization requirement in hexaploid wheat is Vrn-A1. An orthologous vernalization gene, designated Vrn-A m 1, was mapped in the diploid wheat Triticum monococcum between RFLP markers Xwg908 and Xabg702 on the long arm of chromosome 5AmL. The orthology of VrnA m 1 with Vrn-A1 (5A wheat, originally Vrn1), Vrn-D1 (5D wheat, originally Vrn3), Vrn-R1 (5R rye, originally Sp1) and Vrn-H1 (5H barley, originally Sh2) was shown by mapping RFLP markers linked to these vernalization genes on the T. monococcum linkage map. A second vernalization gene, designated Vrn-A m 2, was found in the distal region of chromosome 5AmL within a segment translocated from homoeologous group 4. This gene is completely linked to RFLP marker Xbcd402 and located between the same RFLP markers (Xβ-Amy-1 and Xmwg616) as the Vrn-H2 (originally Sh) locus in Hordeum vulgare.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050978
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  • 67
    Digitale Medien
    Digitale Medien
    A molecular genetic map of the long arm of chromosome 6R of rye incorporating the cereal cyst nematode resistance gene, CreR (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1000-1012 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words CCN ; RFLP ; PCR ; Heterodera avenae ; Genetic mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A genetic map of the long arm of chromosome 6R of rye was constructed using eight homoeologous group-6 RFLP clones and five PCR markers derived from the rye-specific dispersed repetitive DNA family, R173. The map was developed using a novel test-cross F1 (TC-F1) population segregating for resistance to the cereal cyst nematode. Comparisons were made between the map generated with other rye and wheat group-6 chromosome maps by the inclusion of RFLP clones previously mapped in those species. Co-linearity was observed for common loci. This comparison confirmed a dramatic reduction in recombination for chromosome 6R in the TC-F1 population. The CreR locus was included in the linkage map via progeny testing of informative TC-F1 individuals. CreR mapped 3.7 cM distal from the RFLP locus, XksuF37. Comparative mapping should allow the identification of additional RFLP markers more closely linked to the CreR locus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050984
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  • 68
    Digitale Medien
    Digitale Medien
    A Rapid DNA Extraction Method for RFLP and PCR Analysis from a Single Dry Seed (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 90-90 
    Details
    ISSN: 1572-9818
    Schlagwort(e): DNA extration ; DNA fingerprint ; half seed ; PCR ; RFLP ; target gene
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A single-seed DNA extraction method was developed for rapid identification of plant genotype. The method was applied to 12 plant species, including the oil seeds sesame and soybean. The results were comparable to those obtained for oil-less seeds such as rice. This method will be useful for genotypic selection which requires rapid screening of large populations. It can also be used to identify varietal purity of seed stocks by PCR and RFLP analysis. The method includes two major steps, (i) treatment by proteinase K in an SDS extraction buffer, and (ii) grinding of a single half seed in the buffer after incubation. About 1.5–2 µg of DNA per half seed (the endosperm part) of rice was obtained and more than 200 half seed samples could be handled by one person in a day. The DNA could be used for fingerprinting and detection of target genes in a transgenic plant by PCR. The amplified PCR products from the half seed DNA exhibited the same banding patterns as those from leaf DNA. Yield and quality of DNA extracted from half seeds of rice was also sufficient for RFLP analysis. The remnant half seeds containing the embryo can be maintained for later germination of selected genotypes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1007418606098
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  • 69
    Digitale Medien
    Digitale Medien
    Comparative Analysis of Different DNA Extraction Protocols: A Fast, Universal Maxi-Preparation of High Quality Plant DNA for Genetic Evaluation and Phylogenetic Studies (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 69-86 
    Details
    ISSN: 1572-9818
    Schlagwort(e): cpDNA ; DNA extraction ; fingerprinting ; forest trees ; M13 fingerprinting ; method ; PCR ; rDNA ; RFLP ; rhododendron ; plant
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Four DNA extraction protocols were compared for ability to produce DNA from the leaves or needles of several species: oak, elm, pine, fir, poplar and maize (fresh materials) and rhododendron (silica dried or frozen material). With the exception of maize and poplar, the species are known to be difficult for DNA extraction. Two protocols represented classical procedures for lysis and purification, and the other two were a combination of classical lysis followed by anion exchange chromatography. The DNA obtained from all procedures was quantified and tested by PCR and Southern hybridisation.Test results indicated superiority of one of the four protocols; a combination of CTAB lysis followed by anion exchange chromatography which enabled DNA extraction from all seven species. A second protocol also produced DNA from leaves or needles of all species investigated and was well suited for PCR applications but not Southern hybridisations. The remaining protocols produced DNA from some but not all species tested. Abbreviations: CTAB, hexadecyltrimethylammonium bromide; EtOH, Ethanol; TBE, tris-borate-EDTA.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1007428009556
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  • 70
    Digitale Medien
    Digitale Medien
    A new polymorphism in the promoter of the Interleukin 5 receptor alpha subunit (IL-5RA) gene (1998)
    Springer
    Immunogenetics 48 (1998), S. 65-66 
    Details
    ISSN: 1432-1211
    Schlagwort(e): Key words IL-5Rα ; Promoter ; Polymorphism ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002510050402
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  • 71
    Digitale Medien
    Digitale Medien
    Characterisation of Erwinia carotovora subspecies and detection of Erwinia carotovora subsp. atroseptica in potato plants, soil and water extracts with PCR-based methods (1998)
    Springer
    European journal of plant pathology 104 (1998), S. 685-699 
    Details
    ISSN: 1573-8469
    Schlagwort(e): biochemical tests ; DAS-ELISA ; immunomagnetic separation ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A PCR-RFLP test based on a pectate-lyase encoding gene permits the detection of several Erwinia carotovora subspecies, but requires complete DNA extraction. This paper reports on the suitability of a simplified PCR-RFLP protocol to characterise E. carotovora strains and on the performance of PCR, using the same primers, to detect the atroseptica subspecies in substrates of epidemiological significance. A collection of 140 strains from various hosts and geographical origins was characterised for biochemical traits and PCR-RFLPs. PCR performed on boiled bacterial suspensions yielded an amplification product of 434 bp in 109 of the 140 strains. None of the E. carotovora subsp. betavasculorum strains was amplified, even after complete DNA extraction. RFLPs of the PCR product yielded 24 groups, 3 of which were new. Twenty one groups were specific to one subspecies. Several strains biochemically similar to E. carotovora subsp. atroseptica, but growing at 37 °C, showed PCR-RFLP profiles characteristic of E. carotovora subsp. carotovora. Phenetic and cladistic analyses gave three main domains, not strictly related to hosts or geographical origins. The atroseptica (RFLP groups 1 and 2) and wasabiae (group 21) subspecies constituted one of the domains, despite clustering distantly from one another. Host specialisation and molecular homogeneity suggest a clonal structure within these subspecies. Conversely, E. carotovora subsp. odorifera, despite its limited host range and geographical distribution, and E. carotovora subsp. carotovora showed great molecular diversity, spreading respectively across five and 19 RFLP groups. These two subspecies shared RFLP groups 4, 5 and 6. The tree nodes in the phenograms showed a low robustness when bootstrapping the data matrix. PCR coupled with a 48h enrichment step in a polypectate-rich medium improved detection thresholds of E. carotovora subsp. atroseptica (1.5.102- 1.5.103 bacteria/ml in leaves, stems, and tuber peel extracts to 4.107 bacteria/ml in wash water) relative to either immunomagnetic separation coupled with PCR or DAS-ELISA (2.105 in plant samples to 2.107 bacteria/ml in wash water).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008666801839
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  • 72
    Digitale Medien
    Digitale Medien
    Incongruence between RFLPs of chloroplast DNA and morphological classification in east Asian pear (Pyrus spp.) (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 533-539 
    Details
    ISSN: 1573-5109
    Schlagwort(e): chloroplast DNA ; hybridization ; introgression ; Pyrus ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Polymorphism of chloroplast DNA of 106 accessions of Pyrus (mainly east Asian accessions) was examined. Four haplotypes were observed with the combination of three independent restriction site mutations digested by EcoO109 I, Sal I, and Xba I, respectively. In the occidental pear accessions only the most plesiomorphic type was observed, whereas all four types appeared in the oriental pear accessions. This suggests that the oriental species of Pyrus and occidental ones may have evolved independently. The distribution of four haplotypes in the east Asian pear was quite incongruent with the species or infrageneric classification using mainly morphological characters. Considering the high crossability and much occurrence of suspected interspecific hybrids in wild populations, this disaccord is inferred to be the results of the hybridization and introgression between species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008646016181
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  • 73
    Digitale Medien
    Digitale Medien
    Genetic diversity in coconut (Cocos nucifera L.) revealed by restriction fragment length polymorphism (RFLP) markers (1998)
    Springer
    Euphytica 101 (1998), S. 103-108 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Cocos nucifera ; RFLP ; genetic diversity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A study of the genetic diversity in coconut by RFLP analysis was performed in 100 individuals representing 10 Tall and seven Dwarf local populations or 'ecotypes' from various geographical origins. Nine cDNA clones from rice, one mitochondrial DNA clone (CoxI) and one genomic clone (rDNA) from wheat were used as probe for southern hybridization. The distribution of the 40 polymorphic bands revealed by rice cDNA clones was studied using a multivariate analysis and allowed to identify two main genetical groups. The first one includes the ecotypes from the Far East and from the South Pacific, whereas the other one comprises the ecotypes from India, Sri Lanka and Western Africa. The rDNA and the CoxI probes confirm this distinction. The Far East and the Pacific regions which were the most likely center of origin also exhibit the widest polymorphism. The associations between the Panama Tall and the Pacific group and between the West African Tall and the Indian Ocean group reflect their likely origin. The Comoro Tall appears to be intermediate between the two main groups, and could reflect the old migration route between Indonesia and Madagascar. All Dwarf varieties belong to the first group, even those collected in West Africa. Those were probably introduced from Asia and Pacific at the beginning of the century. The cross-pollinating Tall ecotypes were generally more polymorphic than the self pollinating Dwarf ecotypes. The legitimacy of two hybrids between ecotypes was confirmed and maternal inheritance of mitochondrial genome was observed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018323721803
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  • 74
    Digitale Medien
    Digitale Medien
    Loss of genetic diversity associated with selection for resistance to sorghum midge in Australian sorghum (1998)
    Springer
    Euphytica 102 (1998), S. 1-7 
    Details
    ISSN: 1573-5060
    Schlagwort(e): genetic diversity ; linkage drag ; RFLP ; Stenodiplosis sorghicola ; sorghum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract In recent years, hybrids with levels of resistance to sorghum midge (Stenodiplosis sorghicola Coquillett) have become available to Australian sorghum producers. These hybrids have been readily accepted to the extent that more than 80% of the sorghum growing area was planted to hybrids with some level of midge resistance by 1995. Since selection for resistance to sorghum midge is one of the primary objectives of Australian sorghum breeding programs, the relationship between resistance and genetic diversity was investigated. Genetic diversity and heterozygosity were assessed using restriction fragment length polymorphism analysis among 26 grain sorghum hybrids grown commercially in Australia. The genetic distances between each sorghum hybrid and a standard highly resistant hybrid were found to be strongly negatively correlated to hybrid midge resistance ratings (r = - 0.77, p 〈 0.001). In addition, the average heterozygosity of each hybrid was correlated with their midge resistance ratings (r = - 0.54, p 〈 0.01). The results indicate that the move to midge resistant hybrids has been associated with a narrowing of the genetic diversity and average heterozygosity of commercial sorghum hybrids. Repeated use of particular elite parent lines, linkage drag and genetic drift are likely to have contributed to this decline. This reduction in genetic diversity may have implications for the genetic vulnerability of sorghum in Australia and the rate of progress in breeding for yield.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018311908636
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  • 75
    Digitale Medien
    Digitale Medien
    QTL mapping for nodule number and common bacterial blight in Phaseolus vulgaris L. (1998)
    Springer
    Plant and soil 204 (1998), S. 135-145 
    Details
    ISSN: 1573-5036
    Schlagwort(e): chalcone isomerase ; chalcone synthetase ; chitinase ; PAL ; phaseolin ; Phaseolus vulgaris ; QTL ; RFLP ; Rhizobium tropici ; Xanthomonas campestris bv. phaseoli
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A recently developed bean RFLP linkage map was used to identify genetic elements affecting quantitative trait loci (QTLs) in two contrasting common bean genotypes, BAT-93 and Jalo EEP558, under two levels of mineral nitrogen: low – 0.25 mM NH4NO3 and a high – 6 mM NH4NO3. QTLs affecting nodule number (NN) and response to Xanthomonas campestris bv. phaseoli, which causes common bacterial blight (CBB) were identified and mapped. Analyses of 70 F2-derived F3 families, using the F1, the two parents, and a nodulation-defective mutant (Nod-) inoculated with R. tropici UM1899 under both levels of N showed significant differences (P#60;0.0001) among the F3 families for NN. Under low N, three genomic regions influenced both traits, with seven linked markers. In three of the six regions influencing NN, higher NN was associated with the Jalo EEP-558 allele, whereas in only two regions was the BAT-93 allele associated with higher NN. One-way analysis of variance, with each marker as the independent variable and NN as the dependent variable, and interval mapping analysis identified four QTLs, which accounted for 45% of the total variation, and two additional QTLs near to yet unassigned loci. In linkage group D7, one QTL mapped to the same region as a QTL for CBB. Under high N, three additional regions were linked to NN, one where the BAT-93 allele was closely associated with CH18 (chitinase), and the others where the Jalo EEP-558 allele was associated with CHS (chalcone synthetase) and PAL-1 (phenylalanine ammonia lyase). Four regions for CBB were mapped adjacent to or in the same region as a QTL for NN. Thus, N showed dual and opposite effects on the expression of NN and CBB. Analysis of these RFLP markers revealed these ‘hidden’ favorable alleles and can serve as an indirect selection tool to increase NN and resistance to CBB.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1004347515127
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  • 76
    Digitale Medien
    Digitale Medien
    RFLP analysis of a PCR amplified region of chloroplast DNA in eggplant and related Solanum species (1998)
    Springer
    Euphytica 102 (1998), S. 295-299 
    Details
    ISSN: 1573-5060
    Schlagwort(e): chloroplast DNA ; eggplant ; PCR ; RFLP ; Solanum ; taxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract RFLP analysis of a PCR amplified 3.2-kbp region of cpDNA bounded by the conserved sequences in rbc L and ORF 106 was performed in eggplant (Solanum melongena), its related Solanum species, S. incanum, S. virginianum (= S. surattense), S. torvum, S. aethiopicum (= S. gilo), S. aethiopicum (= S. integrifolium), S. violaceum (= S. indicum), S. violaceum (= S. sanitwongsei) and S. mammosum and the reciprocal hybrids between S. aethiopicum (= S. integrifolium) and S. melongena 'Uttara'. The target region of cpDNA was amplified correctly by PCR. The amplified products were digested with each of 10 restriction enzymes (Alu I, Ase I, BamH I, Hinf I, Msp I, Rsa I, ScrF I, Sty I, Taq I and Xba I). Variations of restriction patterns among the species were recognized after digesting the amplified products with each of the seven restriction enzymes, Taq I, Alu I, Rsa I, Sty I, Ase I, Hinf I and Xba I. The restriction patterns divided the examined nine species into the following five clusters, 1) S. melongena and S. incanum, 2) S. virginianum (= S. surattense), 3) S. torvum, 4) S. aethiopicum (= S. gilo), S. aethiopicum (= S. integrifolium), S. violaceum (= S. indicum) and S. violaceum (= S. sanitwongsei) and 5) S. mammosum. The restriction pattern with Alu I in each of the reciprocal hybrids between S. melongena 'Uttara' and S. aethiopicum (= S. integrifolium) was identical with that of seed parent. The present study demonstrated the availability of the PCR-RFLP analysis of cpDNA for assessing taxonomic relationships and identifying cytoplasmic parentage of interspecific hybrids in eggplant and related Solanum species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018304308608
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  • 77
    Digitale Medien
    Digitale Medien
    Identification of genomic regions for rust resistance in sorghum (1998)
    Springer
    Euphytica 103 (1998), S. 287-292 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Sorghum ; genome mapping ; rust ; RFLP ; QTL
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The location and effects of genomic regions for rust resistance in sorghum were determined. One hundred and sixty recombinant inbreds, which derived from a cross between QL39 and QL41, were used as a segregating population for genome mapping and rust resistance evaluation. Phenotypic data were collected in replicated field trials in two years. Interval mapping and non-parametric mapping identified four regions, each in a separate linkage group, associated with rust resistance. The region with the largest effect on rust resistance is on linkage group 10; it accounted for 40% of the total phenotypic variation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018332909752
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  • 78
    Digitale Medien
    Digitale Medien
    Comparative analyses of RFLP diversity in landraces of Triticum aestivum and collections of T. tauschii from China and Southwest Asia (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 312-318 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Genetic diversity ; Triticum tauschii ; Triticum aestivum ; RFLP ; Landrace wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Chinese accessions of Triticum tauschii and T. aestivum L. from the Sichuan white (SW), Yunnan hulled (YH), Tibetan weedrace (TW), and Xinjiang rice (XR) wheat groups were subjected to RFLP analysis. T. tauschii and landraces of T. aestivum from countries in Southwest Asia were also evaluated. For T. tauschii, a west to east gradient was apparent where the Chinese accessions exhibited less diversity than those from Southwest Asia. Compared to the Southwest Asian gene pool, the Chinese T. tauschii was highly homogeneous giving a low frequency of polymorphic bands (16%) and banding patterns (1.33 per probe) with 75 RFLP probe-HindIII combinations. Accessions of T. tauschii from Afghanistan and Pakistan were genetically more similar to the Chinese T. tauschii than those from Iran. Of 368 bands found for 39 Chinese hexaploid wheat accessions with 63 RFLP probe-HindIII combinations, 28.3% were polymorphic with an average of 2.6 banding patterns per probe and 5.0 bands per genotype. The individual Chinese landrace wheat groups revealed less variation than those from Afghanistan, Iran, and Turkey. When classified into country based groups, however, the diversity level over all Chinese landraces was greater than that of some Southwest Asian landraces, especially those from Afghanistan and Iran . The XR wheat group was genetically distinct from the other three Chinese landrace groups and was more related to the Southwest Asian landraces. The TW group was genetically similar to, but more diverse than, the SW and YH groups. The Chinese landraces had a higher degree of genetic relatedness to the Southwest Asian T. tauschii, particularly to accessions from Iran, rather than to the Chinese T. tauschii. ‘Chinese Spring’ was most related to ‘Chengdu-guang-tou’, a cultivar from the SW wheat group.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050742
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  • 79
    Digitale Medien
    Digitale Medien
    Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 170-176 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Wild soybean ; Glycine soja ; RFLP ; Mitochondrial DNA ; Geographic distribution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Wild soybean (Glycine soja Sieb. et Zucc.), regarded as the progenitor of cultivated soybean [G. max (L.) Merr.], is widely distributed in East Asia. We have collected 1097 G. soja plants from all over Japan and analyzed restriction fragment length polymorphisms (RFLPs) of mitochondrial DNA (mtDNA) in them. Based on the RFLPs detected by gel-blot analysis, using coxII and atp6 as probes, the collected plants were divided into 18 groups. Five mtDNA types accounted for 94% of the plants examined. The geographic distribution of mtDNA types revealed that, in many regions, wild soybeans grown in Japan consisted of a mixture of plants with different types of mtDNA, occasionally even within sites. Some of the mtDNA types showed marked geographic clines among the regions. Additionally, some wild soybeans possessed mtDNA types that were identical to those widely detected in cultivated soybeans. Our results suggest that the analysis of mtDNA could resolve the maternal lineage among plants of the genus Glycine subgenus Soja.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050724
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  • 80
    Digitale Medien
    Digitale Medien
    A genetic linkage map of rye (Secale cereale L.) (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 203-208 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Genetic mapping ; Reciprocal crosses ; RFLP ; Rye ; Secale cereale L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A genetic linkage map of rye composed of 91 loci (88 RFLP, two morphological and one isozyme markers) has been developed using two reciprocal crosses. The RFLP loci covering all seven chromosomes were detected by a selection of rye, wheat, barley and oat cDNA and genomic DNA probes. The level of polymorphism was dependent on the source of the clones, with a ranking of rye〉wheat〉barley〉oat. Distorted segregations were detected in linkage groups of chromosomes 1R, 4R, 5R and 7R. When the recombination of the two reciprocal crosses was compared, no systematic increase or decrease in one or the other direction was observed suggesting that a combination of populations of reciprocal crosses is possible.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050728
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  • 81
    Digitale Medien
    Digitale Medien
    Targeted mapping approaches to identify DNA markers linked to the Rfp1 restorer gene for the ‘Polima’ CMS of canola (Brassica napus L.) (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 431-438 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Targeted mapping ; RFLP ; RAPD ; Brassica napus ; Polima CMS ; Nearly isogenic line ; Bulked segregant analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  We have used two targeting approaches [pairs of nearly isogenic lines (NILs) and bulked segregant analysis] to identify DNA markers linked to the Rfp1 restorer gene for the pol CMS of canola (Brassica napus L.). We were able to target the Rfp1 locus as efficiently by comparing NILs as by bulked segregant analysis, and it was demonstrated in this instance that double-screening strategies could significantly improve the overall targeting efficiency. The chance occurrence of shared homozygosity at specific unlinked chromosomal regions in the bulks was found to limit the efficiency of bulked segregant analysis, while the efficiency of NIL comparison was limited by residual DNA from the donor cultivar at scattered sites throughout the genome of the NILs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050913
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  • 82
    Digitale Medien
    Digitale Medien
    Isozyme diversity, RFLP of the rDNA and phylogenetic affinities among cultivated Lima beans,Phaseolus lunatus (Fabaceae) (1998)
    Springer
    Plant systematics and evolution 213 (1998), S. 153-164 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Phaseolus ; Isozymes ; genetic distance ; Lima bean ; rDNA ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Genetic variation inPhaseolus lunatus (Lima bean) was investigated at isozyme and DNA levels. Sixty cultivated accessions, including representatives of the Mesoamerican and Andean gene pools and intermediate types, were analyzed for variability at 17 isozyme loci. Some accessions were also examined for restriction fragment length polymorphism (RFLP) at the rDNA level. These data were used to construct two dendrograms showing clear separation in two distinct groups corresponding to each of the gene pools and an intermediate one probably representing a transitional group.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985196
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  • 83
    Digitale Medien
    Digitale Medien
    A second spontaneous hybrid in the genusLeucaena (Leguminosae, Mimosoideae) (1998)
    Springer
    Plant systematics and evolution 212 (1998), S. 53-77 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Leucaena leucocephala ; L. diversifolia ; L. ×spontanea ; Hybridization ; hybrid detection ; spontaneous hybrid ; RAPD ; RFLP ; chloroplast DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The detection of hybridity inLeucaena is discussed in relation to: (i) traditional criteria, (ii) molecular criteria and (iii) models to predict hybrid leaf morphology. Morphological, geographical and molecular evidence for the occurrence of interspecific hybrids betweenL. leucocephala andL. diversifolia in south-central Mexico, northern Guatemala, Jamaica, Dominican Republic, the Philippines and Papua New Guinea is presented. Predicted mean hybrid leaf trait values calculated from parent material are compared with data from putative hybrids and shown to be similar. The origin of these hybrids is discussed and shown to be the result of artificial sympatry resulting from indigenous, and recent exotic, domestication of the parent species. The hybrid is described asL. ×spontanea.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985221
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  • 84
    Digitale Medien
    Digitale Medien
    Comparison of PCR-RFLP analysis of the ITS region with morphological criteria of various strains of Dunaliella (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 573-580 
    Details
    ISSN: 1573-5176
    Schlagwort(e): Dunaliella ; ITS ; PCR ; RFLP ; strains ; taxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The genus Dunaliella comprises 28 species defined primarily by morphological and physiological criteria, which vary considerably depending on growth conditions. Concomitantly, the taxonomic status of various species is uncertain. To confirm the taxonomic identity and to better understand the relationship within Dunaliella, seven taxa ( D. salina, D. bardawil, D. tertiolecta, D. parva, D. viridis, D. lateralis, D. peircei) were compared using RFLP analysis of the nuclear rDNA repeats, specifically the internal transcribed spacer regions, including the 5.8S rRNA gene. Volvox aureus was used as an outgroup. A single ITS PCR amplification product was obtained for each taxon. An ITS fragment of ca. 640 bp was present in all the taxa within the subgenus Dunaliella, except for D. salina CCMP 1303 (ca. 540 bp) and D. lateralis (subgenus Pascheria) (ca. 600 bp). A cluster analysis based on the presence or absence of bands generated by digestion of the PCR product with 8 restriction endonucleases (DpnI, HhaI, EcoRI, PvuII, TaqI, HaeIII, MspI, StyI) revealed no correlation between the genetic relationship inferred from the ITS-RFLP data and the morpho-physiological attributes used for taxonomy. In addition, differences in morphology, physiology and in the length and restriction fragment patterns of the ITS region of D. salina CCMP 1303 suggest that this strain does not belong to Dunaliella.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008035422784
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  • 85
    Digitale Medien
    Digitale Medien
    Analyses of quantitative trait loci associated with resistance to shape Sclerotinia sclerotiorum in sunflowers (shape Helianthus annuus L.) using molecular markers (1998)
    Springer
    Molecular breeding 4 (1998), S. 215-226 
    Details
    ISSN: 1572-9788
    Schlagwort(e): Helianthus annuus ; QTL ; resistance ; RFLP ; Sclerotinia scerotorum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Restriction fragment length polymorphism and isoenzyme markers were used to investigate quantitative trait loci involved in sunflower resistance to mycelial extension of Sclerotinia sclerotiorum on leaves and capitula. Seed weight, oil content and flowering data were also evaluated. Four quantitative trait loci were demonstrated for leaf resistance and two for capitulum resistance. One of these zones appears involved in resistance to both types of S. sclerotiorum attack while the others appear specific for resistance of one part of the plant. Two quantitative trait loci were detected for seed weight, three for oil content and three for flowering date. Individual quantitative trait loci explained 9% to 48% of the phenotypic variability, confirming the polygenic basis of the quantitative traits studied. Overall, the quantitative trait loci explain 60% of the genetic variation for leaf resistance and 38% for capitulum resistance to S. sclerotiorum. One linkage group is particularly interesting since it includes quantitative trait loci for all the five quantitative traits measured. Hypotheses for linkage versus pleiotropy and consequences of all the results in resistance breeding are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009694626992
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  • 86
    Digitale Medien
    Digitale Medien
    A gene for resistance to the maize streak virus in the African CIMMYT maize inbred line CML202 (1998)
    Springer
    Molecular breeding 4 (1998), S. 147-154 
    Details
    ISSN: 1572-9788
    Schlagwort(e): composite interval mapping ; maize streak virus ; QTL ; RFLP ; Zea mays
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Resistance to maize streak virus (MSV) is an essential trait of improved maize varieties in sub-Saharan Africa. We mapped quantitative trait loci (QTL) for resistance to MSV in a population of 196 F2:3 lines derived from a cross between the maize inbred lines CML202 (resistant) from CIMMYT-Zimbabwe and Lo951 (susceptible) from Italy. Field tests were planted at two locations in Zimbabwe, inoculated with viruliferous leaf hoppers (Cicadulina mbila), and scored twice (21 and 83 days after infesting, DAI) on a 1–5 scale. The mean final streak intensity (score 2) of the parent lines was 2.2 (CML202) and 4.8 (Lo951). Genotype × location interaction was large for score 1 but negligible for score 2. Consequently, the heritability was higher for score 2 (0.93) than for score 1 (0.62). By composite interval mapping across locations, using a linkage map with 110 RFLP loci, four significant (LOD ≥3.0) QTL were identified for score 1 on chromosomes (C) 1, 2, 3, and 4, respectively. All four were contributed by CML202. For score 2, only the QTL on C 1 was significant (LOD =37), explaining 59% of the phenotypic and 64% of the genotypic variance. The QTL's partially dominant gene action was consistent with the nearly intermediate resistance of the F1 generation (relative heterosis for resistance 12%). The presence of one major QTL is consistent with the bimodal frequency distribution of the mapping population showing a clear 3:1 segregation. This gene seems to be allelic or identical to Msv1, a major resistance gene which was previously identified in the same genomic region in Tzi4, an inbred line from IITA. Inbred CML202 had lower final disease ratings than Tzi4. The greater resistance of CML202 may be due to allelic differences at the msv1 locus or due to the minor QTL on C 2, 3, and 4 which were not detected in Tzi4. z y Trigo (International Maize and Wheat Improvement Center); IITA, International Institute of Tropical Agriculture; IRAT, Institute de Recherches Agronomiques Tropicales et des Cultures Vivrières; KARI, Kenya Agricultural Research Institute; MSV, maize streak virus; QTL, quantitative trait locus/loci
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009602620244
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  • 87
    Digitale Medien
    Digitale Medien
    Earthquake induced damage in an historic area: the September–October 1997 seismic sequence which affected Assisi, Central Italy (1998)
    Springer
    Bulletin of engineering geology and the environment 57 (1998), S. 101-109 
    Details
    ISSN: 1435-9537
    Schlagwort(e): Key words Assisi Earthquake ; Seismology ; Historic buildings ; Umbria-Marche ; Seismic microzonation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie
    Beschreibung / Inhaltsverzeichnis: Résumé La sequence sismique de septembre–octobre 1997 qui a affecté l'une des régions historiques les plus importantes d'Italie Centrale a mis en évidence que l'impact économique des tremblements de terre de magnitude modérée peut être considérable. En ce qui concerne la vulnerabilité du patrimoine artistique et architectural, on a constaté que des trésors inestimables pouvaient subir des dégâts importants même lors de secousses modérées. On attire l'attention sur le fait qu'un microzonage sismique ne pourra être efficace que si l'on prend en compte non seulement l'activité sismique passée mais aussi l'historique des développements urbains.
    Notizen: Abstract  The September–October 1997 seismic sequence in one of the most important historic areas of Central Italy showed that the economic impact of earthquakes of only moderate magnitude can be high. In view of the vulnerability of the artistic and architectural heritage, priceless treasures in such towns as Assisi are very susceptible to damage as a consequence of such seismic events. Attention is drawn to the fact that for seismic microzonation to be effective, it is essential that not only the past seismic activity but also the history of the urban developments is taken into account.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s100640050026
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  • 88
    Digitale Medien
    Digitale Medien
    Sequence-tagged-sites (STSs) of cDNA clones in Cryptomeria japonica and their evaluation as molecular markers in conifers (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 764-772 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Linkage map ; RFLP ; STS ; Conifers ; Cryptomeria japonica ; cDNA ; Polymorphism ; Phylogeny ; Homology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  We have generated 66 sequence-tagged-site (STS) markers from cDNA clones of Cryptomeria japonica, and 60% of them have already been mapped into C. japonica linkage groups. All of the STS markers showed a single fragment following polymerase chain reaction (PCR) amplification. We investigated by polymorphism of these STS markers in a mapped F2 population and 15 plus trees by means of a restriction endonuclease analysis. Polymorphism levels were 10.6% and 22.7% in the F2 population and the 15 plus trees, respectively. PCR amplification levels of the 66 STS markers in 14 conifer species varied depending on their genetic relationship with C. japonica. Taxodium, which is closely related to C. japonica, had the most amplifications (31.82%), followed by Sequoiadendron giganteum, which is of the same family. The average proportion of PCR amplifications in each family gradually declined in the following order: from Taxodiaceae to Cuppresaceae, Sciadopityaceae, Pinaceae, and Taxaceae. These results are in general agreement with a molecular phylogenetic relationship based on chloroplast DNA. The 66 STS markers will be useful as on anchor point for genome mapping and population genetics, and some of them will also be useful when studying other conifers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050476
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  • 89
    Digitale Medien
    Digitale Medien
    A RFLP-based linkage map of mustard [Brassica juncea (L.) Czern. and Coss.] (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 841-851 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key wordsBrassica juncea ; Mustard ; Linkage ; map ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A genetic linkage map of Brassica juncea was constructed based on restriction fragment length polymorphism (RFLP) detected by anonymous cDNA markers from B. napus, using a segregating F1-derived doubled haploid (DH) progeny from a cross between a canola-quality mustard line (J90-4317) and a high-oil-content mustard line (J90-2733). The RFLP probes consisted of 229 cDNA probes from B. napus and a B. napus tandem repeat sequence, RDA2. The map consisted of 343 marker loci arranged in 18 major linkage groups plus five small segments with two to five marker loci, covering a total map distance of 2073 cM. Twenty-four percent of the markers were dominant in nature. Sixty-two percent of the marker loci were duplicated, and the majority were involved in inter-linkage group duplications, illustrating that complex duplications and subsequent rearrangements occurred after allopolyploidy. Deviation from the Mendelian segregation ratio for a DH population was observed for 27% of the markers. Two-thirds of these markers with a skewed segregation were clustered in 6 linkage groups and two unassigned segments. The overall average marker interval of the B. juncea map reported here was 6.6 cM, which would provide a marker density satisfactory for efficient use of the map in breeding applications, such as tagging of important agronomic traits and marker-assisted selection.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050485
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  • 90
    Digitale Medien
    Digitale Medien
    Asymmetric fusion between wild and cultivated species of potato (Solanum spp.) –detection of asymmetric hybrids and genome elimination (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 1104-1112 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Asymmetric somatic hybrids ; Flowcytometry ; RFLP ; Solanum tuberosum ; Wild species
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The objective of this study was to evaluate the suitability of different techniques for a simple and rapid identification of asymmetric hybrids, without the use of selection markers and independent of the fusion partners used. Additionally, the degree of donor DNA elimination was determined. Among 473 viable plants obtained from asymmetric fusion experiments between three di-haploid breeding lines of potato (Solanum tuberosum) and diploid wild species (S. bulbocastanum, S. circaeifolium; X-ray treatment of the wild species) the most promising ones were investigated with three different methods: flow cytometry, RFLP analysis with an oligonucleotide probe (GATA)4, and with single-copy probes. Flow cytometry, which combines a high screening capacity with detailed information about the DNA content and allows a distinction between asymmetric hybrids and chimeras, detected 31 hypo-tetraploid and 42 hypo-hexaploid regenerates among 224 plants. With the oligonucleotide probe (GATA)4 only a few asymmetric hybrids were detected among all regenerates. More than 50% of these asymmetric regenerates were chimeras. Concerning the degree of DNA elimination, the results obtained by RFLP analysis with 17 single-copy probes were correlated with the results obtained by flow cytometry. The maximum DNA elimination of the donor genome was 52%. As a trend, an irradiation dosage of 210 Gy caused a higher DNA elimination in the wild species than a dosage of 70 Gy. No calli were obtained after irradiation of the wild species with 420 Gy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050523
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  • 91
    Digitale Medien
    Digitale Medien
    High-resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 153-162 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words PVX ; Resistance gene ; Mapping ; RFLP ; AFLP ; CAPS
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The Rx locus in potato confers extreme resistance to PVX. In the F1 progeny of crosses between the PVX-susceptible cultivar Huinkel and the cultivar Cara (Rx genotype) there was a 1 : 1 segregation of PVX resistance, indicating that Rx in Cara is present in the simplex condition. Using potato and tomato RFLP markers, we mapped Rx in Cara to the distal end of chromosome XII at a different position to the previously mapped Rx1 locus. To generate a high-resolution linkage map in the vicinity of Rx a total 728 AFLP primer combinations were screened using DNA of bulked resistant and susceptible segregants. We also screened segregating populations for chromosomal recombination events linked to the Rx locus and identified 82 plants with recombination events close to Rx. Using these recombinant plants we have identified AFLPs that flank Rx and span an interval of 0.23 cM in a region of the genome where 1 cM corresponds to approximately 400 kb.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050543
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  • 92
    Digitale Medien
    Digitale Medien
    Characterization of Cyanobacteria by SDS-PAGE of whole-cell proteins and PCR/RFLP of the 16S rRNA gene (1997)
    Springer
    Archives of microbiology 168 (1997), S. 176-184 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Key words Cyanobacteria ; Planktonic ; Total protein ; SDS-PAGE ; PCR ; RFLP ; 16S rRNA ; UPGMA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Planktonic, filamentous cyanobacterial strains from different genera, both toxic and nontoxic strains, were characterized by SDS-PAGE of whole-cell proteins and PCR/RFLP of the 16S rRNA gene. Total protein pattern analysis revealed the mutual relationships at the genus level. Restriction fragment length polymorphism (RFLP) of the 16S rRNA gene with reference strains proved to be a good method for the cyanobacterial taxonomy. The nonheterocystous strains outgrouped from the nitrogen-fixing ones. With both methods, Aphanizomenon clustered with Anabaena, and Nodularia with Nostoc. In the RFLP study of Anabaena, the neurotoxic strains were identical, but the hepatotoxic ones formed a heterogeneous group. Genetic distances found in the RFLP study were short, confirming that close genotypic relationships underlie considerable diversity among cyanobacterial genera.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002030050485
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  • 93
    Digitale Medien
    Digitale Medien
    Megaplasmids in Thermus oshimai isolates from two widely separated geographical areas: restriction fragment profiling and DNA homology (1997)
    Springer
    Archives of microbiology 168 (1997), S. 473-479 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Key words Thermus oshimai ; Megaplasmids ; Pulsed-field gel electrophoresis ; RFLP ; Southern ; hybridization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Megaplasmid DNA was detected in ten isolates belonging to the recently described thermophilic eubacterial species Thermus oshimai and isolated from hot springs in Portugal (eight isolates) and Iceland (two isolates). The estimated size of the large plasmids purified from T. oshimai SPS-18 from S. Pedro do Sul, Portugal, and from isolate JK-91 from Hveragerdhi-Hengill, Iceland, was 214 and 275 kb, respectively. No sequence homologous to isolate SPS-18 megaplasmid is present in chromosomal DNA as indicated by Southern hybridization analysis. Overall examination of the HindIII fragment profiles of megaplasmid DNAs purified from isolates from the same geographical area gave similar but not always identical restriction profiles on agarose gels. Restriction fragment length polymorphism (RFLP) was higher for megaplasmids present in isolates purified from the Portuguese and Icelandic isolates than for megaplasmids from the same hot spring. Megaplasmid RFLP correlated with previous results obtained on the polymorphism of macrorestriction patterns of whole genomic DNA and with the RFLP of co-resident small plasmid DNA that was found in one half of the isolates examined. The 16-kb HindIII–HindIII fragment from isolate SPS-18 megaplasmid showed DNA–DNA homology with restriction fragments of similar size generated by the large plasmids present in all the other isolates, even in those from hot springs of widely separated geographical areas. This suggests a high degree of sequence conservation in T. oshimai megaplasmids.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002030050524
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  • 94
    Digitale Medien
    Digitale Medien
    Ribosomal DNA variation in foxtail millet, Setaria italica (L.) P. Beauv., and a survey of variation from Europe and Asia (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 751-756 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Foxtail millet ; rDNA ; RFLP ; Restriction mapping ; Geographical distribution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Restriction fragment length polymorphism (RFLP) and the structure of ribosomal RNA genes (rDNA) were investigated in 117 landraces of foxtail millet, Setaria italica (L.) P. Beauv. Five RFLP phenotypes were found when the genomic DNA was digested with BamHI; these were named types I–V. Of these types I, II and III were the most frequent. Type I was mainly distributed in the temperature zone, type II in the Taiwan-Philippines Islands and type III in South Asia. Restriction mapping of the cloned rDNA and comparison with RFLP phenotypes showed that the different types originated from a polymorphism in the length within the intergenic spacer (IGS) and BamHI site changes within the IGS.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050621
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  • 95
    Digitale Medien
    Digitale Medien
    Genetic variability of the wild diploid wheat Triticum urartu revealed by RFLP and RAPD markers (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 424-430 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Triticum urartu ; Wheat ; A genome ; RFLP ; RAPD ; Genetic variability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Genetic variability among 49 accessions of Triticum urartu was estimated by RFLP and RAPD marker analyses, and the two data sets were compared. One T. timopheevii accession and two accessions of T. durum and T. aestivum, respectively, were included to identify T. urartu accessions closely related to these polyploid wheats. Twenty eight RFLP clones and 29 RAPD primers generated 451 and 155 polymorphic bands, respectively. The three accessions from Armenia clustered together and were well separated from all other accessions, which showed less pronounced geographical patterns. Genetic similarity and co-phenetic values calculated with RAPD markers were very similar to those calculated with RFLP markers for the intraspecific comparisons, but not for the interspecific comparisons. The identification of individual T. urartu accessions which are more related to polyploid wheats than others was not possible.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050432
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  • 96
    Digitale Medien
    Digitale Medien
    Comparison of the genetic maps of Brassica napus and Brassica oleracea (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 569-582 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Brassica napus ; Brassica oleracea ; Genetic maps ; RFLP ; RAPD
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The genus Brassica consists of several hundreds of diploid and amphidiploid species. Most of the diploid species have eight, nine or ten pairs of chromosomes, known respectively as the B, C, and A genomes. Genetic maps were constructed for both B. napus and B. oleracea using mostly RFLP and RAPD markers. For the B. napus linkage map, 274 RFLPs, 66 RAPDs, and two STS loci were arranged in 19 major linkage groups and ten smaller unassigned segments, covering a genetic distance of 2125 cM. A genetic map of B. oleracea was constructed using the same set of RFLP probes and RAPD primers. The B. oleracea map consisted of 270 RFLPs, 31 RAPDs, one STS, three SCARs, one phenotypic and four isozyme marker loci, arranged into nine major linkage groups and four smaller unassigned segments, covering a genetic distance of 1606 cM. Comparison of the B. napus and B. oleracea linkage maps showed that eight out of nine B. oleracea linkage groups were conserved in the B. napus map. There were also regions in the B. oleracea map showing homoeologies with more than one linkage group in the B. napus map. These results provided molecular evidence for B. oleracea, or a closely related 2n=18 Brassica species, as the C-genome progenitor, and also reflected on the homoeology between the A and C genomes in B. napus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050453
    Standort Signatur Erwartet Verfügbarkeit
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  • 97
    Digitale Medien
    Digitale Medien
    RFLP-based estimates of parental contribution to F2- and BC1-derived maize inbreds (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 652-656 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Bootstrap ; Genetic drift ; Parental contribution ; RFLP ; Selection ; Zea mays L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Selection and genetic drift during inbreeding may cause differences between the actual and expected proportions of the genome derived by an inbred from each of its parents. We used 70 RFLP loci to determine the frequency and magnitude of deviations from the expected parental contribution among F2- and BC1-derived maize (Zea mays L.) inbreds. Assuming inbreds i and j were the parents of inbred k, the parental contribution of i to k was estimated as p=(Sik−Sij)/ (1−Sij), where Sik and Sij were the average proportions, across the ten linkage groups in maize, of RFLP loci with alleles common to the inbreds in subscript. Bootstrap confidence intervals (CIs) were obtained for p by re-sampling RFLP similarity for each linkage group. Among 62 F2-derived inbreds, 13 had estimates of p that deviated significantly from the expected value of 0.5. One F2-derived inbred obtained p=0.801 of its genome from a parent. Among 34 BC1-derived inbreds, eight had estimates of p that deviated significantly from the expected contribution of 0.75 from the recurrent parent. Two inbreds, both from the same BC1 population, had an estimated p?0.94. The results suggested that selection during backcrossing generally favored the recurrent parent over the donor parent. Among the inbreds with significant deviations from the expected p, the width of 95% CIs with 70 RFLP loci was 〉0.20. Inbreds selfed from the same F2 or BC1 population varied in p, indicating that coefficients of co-ancestry calculated from pedigree records may give erroneous estimates of genetic relationship.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050462
    Standort Signatur Erwartet Verfügbarkeit
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  • 98
    Digitale Medien
    Digitale Medien
    Chromosomal assignment of the nine linkage groups of sugar beet (Beta vulgaris L.) using primary trisomics (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 590-596 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Beta vulgaris ; Chromosomal assignment ; RFLP ; Primary trisomics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Twenty-four marker loci representing each of the nine linkage groups of sugar beet (Beta vulgaris) have been assigned to the nine primary trisomics of Butterfass (1964). Single-copy RFLP probes were hybridized with filter-bound DNA of the trisomics. The autoradiographs were scanned and analyzed by densitometric methods. Statistics on the integrated optical densities of the RFLP bands revealed a clear relationship of each linkage group to a distinct trisomic type. For the first time each of the linkage groups could unequivocally be assigned to one sugar beet chromosome. A standard nomenclature of the 9 chromosomes of sugar beet is suggested and discussed with respect to previous numbering systems.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050600
    Standort Signatur Erwartet Verfügbarkeit
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  • 99
    Digitale Medien
    Digitale Medien
    The genetics of resistance to five races of downy mildew (Plasmopara halstedii) in sunflower (Helianthus annuus L.) (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 584-589 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Linkage ; Major gene ; Race-specific resistance ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  These studies were undertaken to determine whether downy mildew resistance genes in sunflower were independent as first reported, or linked as suggested by more recent hypotheses. The segregations for downy mildew reaction of 111 F3 progenies from a cross between a susceptible line and a line with Pl2 were used to locate this gene on the sunflower consensus RFLP linkage map. It was shown that Pl2 was linked to the same RFLP markers on linkage group 1 as Pl1 and Pl6, mapped earlier, and at a very similar distance. The F3 progenies showed exactly the same segregation patterns when tested with race 1 and race D. One hundred and fifty four progenies from a cross between a susceptible line and HA335, containing Pl6 (considered as giving resistance to all Plasmopara halstedii races), were tested with the five French downy mildew races, 1, A, B, C and D. Two progenies were observed to show segregation for races 1 and D, while appearing homozygous-resistant to races A , B and C. Tests on F4 progenies confirmed this separation of resistances with fixation of susceptibility to races 1 and D and resistance to races A, B and C. It is concluded that the Pl6 gene is not a “strong” gene, giving resistance to all downy mildew races, but rather a cluster of genes, each providing resistance to one, or a few, downy mildew races. The genes giving resistance to races 1 and D, on one hand, and to races A, B and C, on the other hand, must be very closely linked, with about 0.6 cM between the two groups.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050599
    Standort Signatur Erwartet Verfügbarkeit
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  • 100
    Digitale Medien
    Digitale Medien
    Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 622-628 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Gene mapping ; RAPD ; RFLP ; Stripe rust ; Triticum dicoccoides ; Triticum durum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The Yr15 gene of wheat confers resistance to the stripe rust pathogen Puccinia striiformis West., which is one of the most devastating diseases of wheat throughout the world. In the present study, molecular markers flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines approach. RFLP screening of 76 probe-enzyme combinations revealed one polymorphic marker (Nor/TaqI) between the susceptible and the resistant lines. In addition, out of 340 RAPD primers tested, six produced polymorphic RAPD bands between the susceptible and the resistant lines. The genetic linkage of the polymorphic markers was tested on segregating F2 population (123 plants) derived from crosses between stripe rust-susceptible Triticum durum wheat, cv D447, and a BC3F9 resistant line carrying Yr15 in a D447 background. A 2.8-kb fragment produced by the Nor RFLP probe and a 1420-bp PCR product generated by the RAPD primer OPB13 showed linkage, in coupling, with the Yr15 gene. Employing the standard maximum-likelihood technique it was found that the order OPB13 1420 –Yr15–Nor1 on chromosome 1B appeared to be no less than 1000-times more probable than the closest alternative. The map distances between OPB13 1420 –Yr15–Nor1 are 27.1 cM and 11.0 cM for the first and second intervals, respectively. The application of marker-assisted selection for the breeding of new wheat cultivars with the stripe rust resistance gene is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050604
    Standort Signatur Erwartet Verfügbarkeit
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