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  • Immunocytochemistry  (352)
  • Wheat  (219)
  • Springer  (571)
  • American Chemical Society
  • Periodicals Archive Online (PAO)
  • 1985-1989  (314)
  • 1980-1984  (257)
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  • Springer  (571)
  • American Chemical Society
  • Periodicals Archive Online (PAO)
  • Wiley-Blackwell  (13)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Occurrence, distribution and nature of neuropeptide Y in the rat pancreas (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1554-1557 
    Details
    ISSN: 1420-9071
    Keywords: Immunocytochemistry ; neuropeptide Y ; radioimmunoassay ; rat pancreas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Significant quantities of a newly discovered peptide, neuropeptide Y, were found in the rat pancreas, where they were localized to nerves in the exocrine parenchyma and around arterial and ductal structures. Although unaffected by surgical parasympathectomy, the periarterial and periductal nerves were abolished by chemical sympathectomy, suggesting that NPY is partially costored with sympathetic transmitters in nerve fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01964804
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation of nitrogen and phosphorus-enriched paddy straw compost and its effect on yield and nutrient uptake by wheat (Triticum aestivum L.) (1989)
    Springer
    Biology and fertility of soils 8 (1989), S. 339-342 
    Details
    ISSN: 1432-0789
    Keywords: Wheat ; Paddy straw compost ; N and P enrichment ; Rock phosphate ; Pyrite ; Triticum aestivum ; Nutrient uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A nutrient-rich compost from paddy straw was prepared using urea and Mussoorie rock phosphate for N and P enrichment respectively. Inorganic N was partly conserved in the compost by the addition of pyrite. Citric-acid-soluble P also increased with the addition of pyrite. Compost containing about 1.6% total N and 3.3% total P was found to be a good source of P for a wheat crop and also supplied a significant amount of N to the plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263166
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of herbicides on three vesicular-arbuscular fungi associated with winter wheat (Riticum aestivum L.) (1989)
    Springer
    Biology and fertility of soils 7 (1989), S. 113-119 
    Details
    ISSN: 1432-0789
    Keywords: Vesicular-arbuscular mycorrhiza ; Herbicides ; Wheat ; Glomus spp. ; Spore germination ; Root infection ; Triticum aestivum L. ; Bifenox ; Mecoprop ; Difenzoquat methyl sulphate ; Chlortoluron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We studied the effects of field application rates of four herbicides (Avenge, Ceridor, Dicurane, and Harrier) on spore germination and infection of wheat roots by three species of Glomus grown under conditions of low P availability. Low concentrations of Ceridor (bifenox, mecoprop) and Harrier (mecoprop, ioxynil, clopyralid) inhibited spore germination while higher concentrations were stimulatory. Avenge (difenzoquat methyl sulphate) prevented spore germination completely, while Dicurane (chlortoluron) had no effect. The herbicide applications had no significant effect on the infection rates of any of the three fungi except Harrier on G. geosporum. The herbicide treatments did, however, affect plant growth and ear yields. Ceridor and Harrier increased ear yields, while Dicurane showed marked phytotoxic effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292568
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  • 4
    Electronic Resource
    Electronic Resource
    Crop growth and nitrogen transformations in wheat (Triticum aestivum L.) planted after wetland rice (Oryza sativa L.) (1989)
    Springer
    Biology and fertility of soils 7 (1989), S. 263-268 
    Details
    ISSN: 1432-0789
    Keywords: Dentrification ; Immobilization ; Tillage ; N leaching ; Wheat ; Wetland rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A field study was undertaken to examine the effects of various management strategies on wheat (Triticum aestivum L.) performance and N cycling in an intensively cropped soil. Microplots receiving 100 kg N ha−1 as15NH4 + 15NO3 − at sowing, tillering or stem elongation were compared with unfertilized microplots. Stubble from the previous rice crop was either incorporated, burnt without tillage, burnt then tilled or retained on the surface of untilled soil. Wheat grain yield ranged from 1.5 to 5.1 t ha− and was closely related to N uptake. Plant accumulation of soil N averaged 36 kg N ha−1 (LSD 5% = 10) on stubble-incorporation plots and 54 kg N ha−1 on stubble-retention plots. Fertilizer N accumulation averaged 18 kg N ha−1 (LSD 51% = 6) on stubble-incorporation plots and 50 kg N ha−1 on stubble-retention plots. Tillage had little effect on burnt plots. Delaying N application from sowing until stem elongation increased average fertilizer N uptake from 26 to 39 kg N ha−1 (LSD 5% = 6), but reduced soil N uptake from 50 to 37 kg N ha− (LSD 5% = 10). Immobilization and leaching did not vary greatly between treatments and approximately one-third of the fertilizer was immobilized. Less than 1% of the fertilizer was found below a depth of 300 mm. Incorporating 9 t ha−1 of rice stubble 13 days before wheat sowing reduced net apparent mineralization of native soil N from 37 to 3 kg ha−1 between tillering and maturity. It also increased apparent denitrification of fertilizer N from an average 34 to 53 kg N ha−1 (LSD 5% = 6). N loss occurred over several months, suggesting that denitrification was maintained by continued release of metabolizable carbohydrate from the decaying rice stubble. The results demonstrate that no-till systems increase crop yield and use of both fertilizer and soil N in intensive rice-based rotations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00709659
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  • 5
    Electronic Resource
    Electronic Resource
    Varietal and fertilizer effects on straw phytotoxins (1986)
    Springer
    Biology and fertility of soils 2 (1986), S. 109-112 
    Details
    ISSN: 1432-0789
    Keywords: Straw ; Phytotoxins ; Acetic Acid ; Wheat ; Barley ; Cultivar ; Nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Acetic acid production over 10 days from fresh barley straw was greater than that from fresh wheat straw, but there were only minor consistent differences between different cultivars. The effect depended on the soil type on which the straw had been produced and acid production was greatest with the largest amounts of N fertilizer which had been used. The fertilizer also affected the C:N ratio of the straw
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257588
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of wheat (Triticum aestivum) roots on mineralization rates of soil organic matter (1988)
    Springer
    Biology and fertility of soils 7 (1988), S. 67-70 
    Details
    ISSN: 1432-0789
    Keywords: Root activity ; Soil C mineralization ; 14C-labelled plant material ; Decomposition stages ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two different soils were amended with 14C-labelled plant material and incubated under controlled laboratory conditions for 2 years. Half the samples were cropped with wheat (Triticum aestivum) 10 times in succession. At flowering, the wheat was harvested and the roots removed from the soil, and a new crop was started. Thus, the soil was continuously occupied by predominantly active root systems. The remaining samples were maintained without plants under the same conditions. The aim of the experiment was to study the effects of active roots on C-mineralization rates during different stages of decomposition and during long-term incubation. During the first 200 days, corresponding to the active decomposition stages, the roots weakly reduced 14C mineralization. With a lower level of decomposition, when more than 60% of the initial 14C was mineralized and when the available nutrients were markedly exhausted by plant uptake, the roots stimulated 14C mineralization.[/ p]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260735
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  • 7
    Electronic Resource
    Electronic Resource
    Root activity and carbon metabolism in soils (1988)
    Springer
    Biology and fertility of soils 7 (1988), S. 71-78 
    Details
    ISSN: 1432-0789
    Keywords: Root activity ; Rhizosphere ; C metabolism ; Microbial biomass ; Microbial activity ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two different soils were amended with 14C-labelled plant material and incubated under controlled laboratory conditions for 2 years. Half the samples were cropped with wheat (Triticum aestivum) 10 times in succession. At flowering, the wheat was harvested and the old roots removed from the soil, so that the soil was continuously occupied by predominantly active root systems. The remaining samples were maintained without plants under the same conditions. During the initial stages of high microbial activity, due to decomposition of the labile compounds, the size of the total microbial biomass was comparable for both treatments, and the metabolic quotient (qCO2-C = mg CO2-C·mg−1 Biomass C·h−1) was increased by the plants. During the subsequent low-activity decomposition stages, after the labile compounds had been progressively mineralized, the biomass was multiplied by a factor of 2–4 in the presence of plants compared to the bare soils. Nevertheless, qCO2-C tended to reach similar low values with both treatments. The 14C-labelled biomass was reduced by the presence of roots and qCO2-14C was increased. The significance of these results obtained from a model experiment is discussed in terms of (1) the variation in the substrate originating from the roots and controlled by the plant physiology, (2) nutrient availability for plants and microorganisms, (3) soil biotic capacities and (4) increased microbial turnover rates induced by the roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260736
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  • 8
    Electronic Resource
    Electronic Resource
    Inoculation of field-grown wheat (Triticum aestivum) with Azospirillum spp. in Brazil (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 37-40 
    Details
    ISSN: 1432-0789
    Keywords: Azospirillum brasilense ; Azospirillum amazonense ; rate reductase ; Inoculation ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three field experiments with wheat were conducted in 1983, 1984, and 1985 in Terra Roxa soil in Paraná, the major Brazilian wheat-growing region, to study inoculation effects of various strains of Azospirillum brasilense and A. amazonense. In all three experiments inoculation with A. brasilense Sp 245 isolated from surface-sterilized wheat roots in Paraná produced the highest plant dry weights and highest N% in plant tops and grain. Grain yield increases with this strain were up to 31 % but were not significant. The application of 60 or 100 kg N ha−1 to the controls increased N accumulation and produced yields less than inoculation with this strain. Another A. brasilense strain from surface-sterilized wheat roots (Sp 107st) also produced increased N assimilation at the lower N fertilizer level but reduced dry weights at the high N level, while strain Sp 7 + Cd reduced dry weights and N% in the straw at both N levels. The A. amazonense strain isolated from washed roots and a nitrate reductase negative mutant of strain Sp 245 were ineffective. Strains Sp 245 and Sp 107st showed the best establishment within roots while strain Cd established only in the soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280348
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  • 9
    Electronic Resource
    Electronic Resource
    Decomposition of wheat and barley straw treated with urea-sulfuric acid (1987)
    Springer
    Biology and fertility of soils 4 (1987), S. 199-203 
    Details
    ISSN: 1432-0789
    Keywords: Wheat ; Barley ; Urea ; Sulfuric acid ; Straw decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat straw treated with 0.5 or 1.0 ml/g urea-sulfuric acid (1:1 acid in water v/v) and incubated in Protneuf or Woodburn silt loam soils in the laboratory decomposed faster than nontreated straw the first 4–6 weeks but at 12 weeks the nontreated straw had decomposed 25%–45% more. In a field experiment, urea-sulfuric acid treated straw, removed at 40-day intervals over 160 days, decomposed faster than nontreated straw. The differences were attributed to salt buildup in the laboratory samples, where electrical conductivities up to 17.6 dS/m were observed. In the field, leaching removed the excess salts. Nitrification produced up to 1875 mg NO 3 − N/kg Portneuf silt loam soil in the laboratory, indicating that nitrifying bacteria were not suppressed by the salt. Total plate counts with no straw were 1.8 × 106 microorganisms/g and with urea-sulfuric acid treated straw were 15.7 × 106/g soil after 14 days incubation. The respective actinomycete counts were 0.3 × 106 and 6.7 × 106/g for the no straw and straw-treated soils, respectively. The urea-sulfuric acid treatments suppressed straw decomposition in the laboratory and accelerated straw decomposition in the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270941
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  • 10
    Electronic Resource
    Electronic Resource
    Immunocytochemical location of vitellin in the egg of the silkworm,Bombyx mori, during early developmental stages (1983)
    Springer
    Development genes and evolution 192 (1983), S. 113-119 
    Details
    ISSN: 1432-041X
    Keywords: Vitellin ; Yolk granule ; Yolk protein ; Silkworm ; Embryogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vitellin was purified from eggs of the silkworm,Bombyx mori, by a new method in which vitellin was extracted from isolated yolk granules. The purified vitellin had a molecular weight of 540,000. An antibody against purified vitellin was prepared in rabbits. It reacted with the hemolymph vitellogenin as well as with purified vitellin, but not with other proteins in the hemolymph or in the extract from yolk granules. The anti-vitellin IgG was used to immunocytochemically locate vitellin in theBombyx non-diapause egg during early developmental stages. In the egg, just after oviposition, vitellin was located in internal yolk granules and in small yolk granules of the periplasm. During the early developmental stages studied, vitellin was not metabolized uniformly throughout the egg. The vitellin of the internal yolk granules located at the posterior-dorsal part and of the small peripheral yolk granules was utilized in 16 h and 2 days, respectively, after oviposition. A thin, very vitellin-poor layer was located between the periplasm and the vitellin-rich interior in the newly laid egg. it was always in close contact with the periphery where blastoderm and germ-band cells developed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848679
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  • 11
    Electronic Resource
    Electronic Resource
    Immunocytochemical and electrophoretic studies on the localization of the major haemolymph proteins (ceratitins) inCeratitis capitata during development (1984)
    Springer
    Development genes and evolution 194 (1984), S. 37-43 
    Details
    ISSN: 1432-041X
    Keywords: Major haemolymph proteins ; Development ; Cuticle ; Immunocytochemistry ; Ceratitis capitata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental profile of the major haemolymph proteins (ceratitins) inCeratitis capitata was studied. Ceratitin concentration in the haemolymph decreases dramatically during the last days of pupal life, while the amounts of ceratitins in whole organism extracts remain unchanged. By electrophoretic, immunological and immunofluorescence techniques it was revealed that ceratitins are reabsorbed by the fat body and a fraction of them is deposited in the cuticle. The possible role of ceratitins is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848952
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  • 12
    Electronic Resource
    Electronic Resource
    Immunocytochemical demonstration of calmodulin in cells secreting by exocytosis (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1340-1342 
    Details
    ISSN: 1420-9071
    Keywords: Immunocytochemistry ; calmodulin ; secretory granules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Calmodulin is a regulator of several calcium-dependent cellular processes. It has been suggested that it plays a role in the mechanism of secretion. Employing an indirect immunoperoxidase technique at the light microscope level, this study demonstrates the presence of calmodulin in several exocytotic cells (mast cells, thyroid follicular cells, neurohypophyseal neurosecretory terminals, pancreaticβ-cells and pancreatic acinus cells) in rat and man. The positive staining reaction for calmodulin was granular and at least in the case of rat mast cells it appeared to be associated with the granule membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01952085
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  • 13
    Electronic Resource
    Electronic Resource
    Localization of phytohemagglutinin in the embryonic axis of Phaseolus vulgaris with ultra-thin cryosections embedded in plastic after indirect immunolabeling (1984)
    Springer
    Planta 162 (1984), S. 548-555 
    Details
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; Lectin (localization) ; Phaseolus (lectin) ; Phytohemagglutinin ; Seed (lectin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined the properties and subcellular localization of phytohemagglutinin (PHA), the major lectin of the common bean (Phaseolus vulgaris.), in the axis cells of nearly mature and imbibed mature seeds. On a protein basis the axis contained about 15% as much PHA as the cotyledons. Localization of PHA was done with an indirect immunolabeling method (rabbit antibodies against PHA, followed by colloidal gold particles coated with goat antibodies against rabbit immunoglobulins) on ultra-thin cryosections which were embedded in plastic on the grids after the immunolabeling procedure. The embedding greatly improved the visualization of the subcellular structures. The small (4 nm) collodial gold particles, localized with the electron microscope, were found exclusively over small vacuoles or protein bodies in all the cell types examined (cortical parenchyma cells, vascular-bundle cells, epidermal cells). The matrix of these vacuoles-protein bodies appears considerably less dense than that of the protein bodies in the cotyledons, but the results confirm that in all parts of the embryo PHA is localized in similar structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00399921
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  • 14
    Electronic Resource
    Electronic Resource
    Immunochemical studies on xanthine dehydrogenase of soybean root nodules (1986)
    Springer
    Planta 167 (1986), S. 190-195 
    Details
    ISSN: 1432-2048
    Keywords: Glycine (xanthine dehydrogenase) ; Immunocytochemistry ; Polyclonal antibody ; Root nodule ; Xanthine dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Xanthine dehydrogenase (XDH, EC 1.2.1.37) was purified from root nodules of soybean (Glycine max) and used to prepare a polyclonal rabbit antiserum. Monospecificity of this antiserum was ascertained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipate. During root nodule development of soybean, only one form of XDH was detected on an immunological basis. Titration of XDH by immunoelectrophoresis showed that a remarkable increase in the amount of XDH occurred between two and four weeks after inoculation, in parallel with the increase in enzyme activity. Localization of XDH by immunofluorescence indicated that the enzyme was present exclusively in uninfected cells where it appeared to be associated with discrete organellels
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391414
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  • 15
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of reserve protein in the endoplasmic reticulum of developing bean (Phaseolus vulgaris) cotyledons (1980)
    Springer
    Planta 150 (1980), S. 419-425 
    Details
    ISSN: 1432-2048
    Keywords: Cotyledons ; Endoplasmic reticulum ; Ferritin labeling ; Immunocytochemistry ; Phaseolus ; Protein (reserve) ; Reserve protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructure of the storage parenchyma cells of the cotyledons of developing bean (Phaseolus vulgaris L.) seeds was examined in ultrathin frozen sections of specimens fixed in a mixture of glutaraldehyde, formaldehyde and acrolein, infused with 1 M sucrose, and sectioned at-80° C. Ultrastructural preservation was excellent and the various subcellular organelles could readily be identified in sections which had been stained with uranyl acetate and embedded in Carbowax and methylcellulose. The cells contained large protein bodies, numerous long endoplasmic reticulum cisternae, mitochondria, dictyosomes, and electron-dense vesicles ranging in size from 0.2 to 1.0 μm. Indirect immunolabelling using rabbit immunoglobulin G against purified phaseolin (7S reserve protein), and ferritin-conjugated goat immunoglobulin G against rabbit immunoglobulin G was used to localize phaseolin. With a concentration of 0.1 mg/ml of anti-phaseolin immunoglobin G, heavy labeling with ferritin particles was observed ober the protein bodies, the cisternae of the endoplasmic reticulum, and the vesicles. The same structures were lightly labeled when the concentration of the primary antigen was 0.02 mg/ml. Ferritin particles were also found over the Golgi bodies. The absence of ferritin particles from other organelles such as mitochondria and from areas of cytoplasm devoid of organelles indicated the specificity of the staining, especially at the lower concentration of anti-phaseolin immunoglobulin G.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390179
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  • 16
    Electronic Resource
    Electronic Resource
    On the cellular localization of phosphoenolpyruvate carboxylase in Sorghum leaves (1981)
    Springer
    Planta 151 (1981), S. 226-231 
    Details
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; (PEP carboxylase) ; PEP carboxylase ; Sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is predominantly located in the cytoplasm of mesophyll cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395173
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  • 17
    Electronic Resource
    Electronic Resource
    Localisation of genes for components of photosystem II in chloroplast DNA from pea and wheat (1985)
    Springer
    Current genetics 10 (1985), S. 329-333 
    Details
    ISSN: 1432-0983
    Keywords: Pea ; Wheat ; Chloroplast genes ; Photosystem II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genes for three components of photosystem II have been localised in chloroplast DNA from pea and wheat by hybridisation with gene-internal sequences from spinach chloroplast DNA. In both pea and wheat, the gene for the 51 kDa polypeptide is located close to the genes for cytochrome b-563 and the 15 kDa polypeptide of the cytochrome b-f complex. The genes for the D2 and 44 kDa polypeptides are located close together, approximately 55 kbp from the gene for the 51 kDa polypeptide, in both pea and wheat chloroplast DNA. The location and orientation of the genes for the D2 and 44 kDa polypeptides in wheat chloroplast DNA indicate that the rearrangement of the wheat genome with respect to the spinach genome is the result of at least two inversions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365629
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  • 18
    Electronic Resource
    Electronic Resource
    Homology in the region containing a tRNATrp gene and a (complete or partial) tRNAPro gene in wheat mitochondrial and chloroplast genomes (1987)
    Springer
    Current genetics 12 (1987), S. 91-98 
    Details
    ISSN: 1432-0983
    Keywords: Organellar genomes ; tRNA genes ; Wheat ; Maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have used bean mitochondrial (mt) and chloroplast (cp) tRNATrp as probes to locate the corresponding genes on the mt and cp genomes of wheat and we have determined the nucleotide sequences of the wheat mt and cp tRNATrp genes and of the flanking regions. Sequence comparisons show that the wheat mt and cp tRNATrp genes are 97% homologous. On the wheat cp DNA, a tRNA Pro UGG gene was found 139 by upstream of the cp tRNATrp gene. On the wheat mt DNA, a sequence of 23 nucleotides completely homologous with the 3' end of this cp tRNAPro gene was found 136 by upstream of the rut tRNATrp gene, but there is only 38% homology between cp and mt wheat genomes in the intergenic regions. The overall organization of this region in the chloroplast genome (a tRNATrp gene separated by about 140 by from a tRNAPro gene) is also found in the mitochondrial genome, suggesting that this mitochondrial fragment might have originated from a chloroplast DNA insertion. A comparison of the genes and of the intergenic regions located between the tRNATrp gene and the tRNAPro (or partial tRNAPro) gene shows that there is an almost complete conservation of these sequences in the mitochondrial DNA of wheat and maize, whereas wheat mt and cp intergenic regions show more sequence divergence. Wheat mt tRNATrp gene is encoded by the main mt genome (accounted for by the master chromosome) but, in the case of maize mitochondria, this gene was found to be encoded by the 2.3 kb linear plasmid, indicating that this plasmid is not dispensable in maize mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00434662
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  • 19
    Electronic Resource
    Electronic Resource
    Extracellular protein fibrils in Chrysochromulina breviturrita (Prymnesiophyceae) and their serological relationship to fungal fimbriae (1986)
    Springer
    Archives of microbiology 146 (1986), S. 207-213 
    Details
    ISSN: 1432-072X
    Keywords: Extracellular proteins ; Surface fibrils ; Algae-fungi-Chrysochromulina ; Immunocytochemistry ; Agglutination ; Fimoriae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An extensive network of extracellular fibrils was revealed by negative staining in the greenish gold algal flagellate, Chrysochromulina breviturrita. These fibrils were of uniform diameter (4–5 nm), sometimes exceeding 5 μm in length. In addition there were short, narrower fibrils (2–3 nm) on the surface of the flagella. Six protein bands were isolated from spent culture medium by SDS-PAGE and one of 80,000 Da was found to polymerize after dialysis into 4–5 nm fibrils identical to those found on the cell surface. Two other proteins of 58,000 Da and 65,000 Da also formed 4–5 nm fibrils but these were either rare or of a shorter length and different appearance. An antiserum directed against the surface 7 nm fibrils (fimbriae) of fungi agglutinated cells of C. breviturrita and some other Prymnesiophyceae and Chrysophyceae, but did not agglutinate cells of algal species in other groups. Immunofluorescence and protein A gold labelling confirmed that antigens related to fungal fimbriae were present on the surface of cells of C. breviturrita. Only the 80,000 and 58,000 Da proteins labelled heavily following protein A gold labelling. Some individual 4–5 nm fibrils labelled with gold were observed in the material prepared from the 80,000 Da band. These results therefore establish that C. breviturrita produces a surface network of fibrils that are serologically related to the fimbriae of fungi, and suggest a previously unrecognized relationship between members of the Prymnesiophyceae, Chrysophyceae and fungal groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403218
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    Immunocytochemical localization of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum (1987)
    Springer
    Archives of microbiology 147 (1987), S. 190-194 
    Details
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Methyl-CoM reductase ; Immunocytochemistry ; Colloidal gold ; Energy conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of Methanobacterium thermoautotrophicum were fixed with glutaraldehyde, sectioned and labeled with antibodies against the β subunit of component C (=methyl-CoM reductase) of methyl-CoM reductase system and with colloidal gold-labeled protein A. It was found that the gold particles were located predominantly in the vicinity of the cytoplasmic membrane, when the cells were grown under conditions where methyl-CoM reductase was not overproduced. This finding confirms the recent data obtained with Methanococcus voltae showing via the same immunocytochemical localization technique that in this organism methyl-CoM reductase is membrane associated.
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    URL: http://dx.doi.org/10.1007/BF00415283
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    Immunocytochemical localization of two key enzymes of the 2-hydroxyglutarate pathway of glutamate fermentation in Acidaminococcus fermentans (1988)
    Springer
    Archives of microbiology 150 (1988), S. 504-508 
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    ISSN: 1432-072X
    Keywords: Acidaminococcus fermentans ; Glutamate fermentation ; Electron microscopy ; Immunocytochemistry ; Post-embedding labelling ; Antibody-gold complexes ; Protein A-gold complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the in situ location of glutaconyl-CoA decarboxylase and 2-htdroxyglutaryl-CoA dehydratase in Acidaminococcus fermentans using the antibody-gold and protein A-gold techniques carried out as a post-embedding immunoelectron microscopic procedure. Polyclonal antisera were raised in rabbits against homogeneous fractions of the enzymes. Anaerobically grown cells of A. fermentans of the late exponential growth phase were fixed with 0.2% glutaraldehyde and 0.3% formaldehyde (final concentrations) in the growth medium. Dehydration of the cells was achieved with methanol. The cells were embedded in the low temperature embedding resin Lowicryl K4M. The markers indicative for antigenic sites of the two enzymes unequivocally demonstrate that the sodium pump glutaconyl-CoA decarboxylase is located at the cell periphery being a membrane-bound enzyme as expected whereas 2-hydroxyglutaryl-CoA dehydratase is a soluble cytoplasmic enzyme.
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    URL: http://dx.doi.org/10.1007/BF00422295
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    Dihydroxyacetone synthase is localized in the peroxisomal matrix of methanol-grown Hansenula polymorpha (1985)
    Springer
    Archives of microbiology 143 (1985), S. 237-243 
    Details
    ISSN: 1432-072X
    Keywords: Hansenula polymorpha ; Peroxisomes ; Methanol ; Dihydroxyacetone synthase ; Cell fractionation ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The subcellular localization of dihydroxyacetone synthase (DHAS) in the methylotrophic yeast Hansenula polymorpha was studied by various biochemical and immunocytochemical methods. After cell fractionation involving differential and sucrose gradient centrifugation of protoplast homogenates prepared from methanol-grown cells, DHAS cosedimented with the peroxisomal enzymes alcohol oxidase and catalase. Electron microscopy of this fraction showed that it contained mainly intact peroxisomes, whereas SDS-polyacrylamide gel electrophoresis revealed two major protein bands (75 and 78 kDa) which were identified as alcohol oxidase and DHAS, respectively. The localization of DHAS in peroxisomes was further established by immunocytochemistry. After immuno-gold staining carried out on ultrathin sections of methanol-grown H. polymorpha using DHAS-specific antibodies, labelling was confined to the peroxisomal matrix.
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    URL: http://dx.doi.org/10.1007/BF00411242
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    Localization of nitrogenase in vesicles of Frankia sp. Cc1.17 by immunogoldlabelling on ultrathin cryosections (1987)
    Springer
    Archives of microbiology 146 (1987), S. 327-331 
    Details
    ISSN: 1432-072X
    Keywords: Actinomycetes ; Nitrogen fixation ; Symbiosis ; Immunocytochemistry ; Ultracryotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunogoldlabelling on ultrathin cryosections of Frankia sp. Cc1.17 showed specific labelling of nitrogenase in the spherical cells called vesicles. No label was found in the hyphae in any cells grown on a medium with combined nitrogen, nor in those to which no specific antiserum was added. Similar results were obtained with cultures grown under high (20%) and low (2%) oxygen tension in the gas phase.
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    URL: http://dx.doi.org/10.1007/BF00410930
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    The major albumin protein from pea (Pisum sativum L.) (1985)
    Springer
    Planta 165 (1985), S. 522-526 
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    ISSN: 1432-2048
    Keywords: Albumin (localisation) ; Cotyledon ; Pisum (albumin protein) ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The major albumin protein in storage parenchyma tissue of developing peas has been localised at an ultrastructural level by immunocytochemistry. Tissue was fixed in buffered aldehyde and embedded in LR White resin which was polymerised by addition of catalyst. Sections were labelled by the indirect method of absorption of Protein A-gold to specifically bound antibodies. This method gives high levels of specific labelling on sections which retain good ultrastructural preservation and have high contrast after conventional staining. The albumin is located throughout the cytoplasm although no labelling was found associated with the endoplasmic reticulum, Golgi apparatus, vacuoles-protein bodies or other organelles.
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    URL: http://dx.doi.org/10.1007/BF00398098
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    Immunocytochemical localization of phaseolin and phytohemagglutinin in the endoplasmic reticulum and Golgi complex of developing bean cotyledons (1985)
    Springer
    Planta 164 (1985), S. 295-302 
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    ISSN: 1432-2048
    Keywords: Cotyledon ; Golgi complex ; Immunocytochemistry ; Phaseolus (seed proteins) ; Phaseolin ; Phytohemagglutinin ; Protein (seeds)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Development of legume seeds is accompanied by the synthesis of storage proteins and lectins, and the deposition of these proteins in protein-storage vacuoles (protein bodies). We examined the subcellular distribution, in developing seeds of the common bean, Phaseolus vulgaris L., of the major storage protein (phaseolin) and the major lectin (phytohemagglutinin, PHA). The proteins were localized using an indirect immunocytochemical method in which ultrathin frozen sections were immunolabeled with rabbit antibodies specific for either PHA or phaseolin. Bound antibodies were then localized using goat-anti-rabbit immunoglobulin G adsorbed onto 4- to 5-nm colloidal gold particles. The sections were post-fixed with OsO4, dehydrated, and embedded in plastic on the grids. Both PHA and phaseolin exhibited a similar distribution in the storage-parenchyma cells, being found primarily in the developing protein bodies. Endoplasmic reticulum and Golgi complexes (cisternal stacks and associated vesicles) also were specifically labeled for both proteins, whereas the cytosol and other organelles, such as mitochondria, were not. We interpret these observations as supporting the hypothesis that the transport of storage proteins and lectins from their site of synthesis, the rough endoplasmic reticulum, to their site of deposition, the protein bodies, is mediated by the Golgi complex.
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    URL: http://dx.doi.org/10.1007/BF00402940
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    Efficient production of haploid wheat (Triticum aestivum) through crosses between Japanese wheat and maize (Zea mays) (1989)
    Springer
    Plant cell reports 8 (1989), S. 263-266 
    Details
    ISSN: 1432-203X
    Keywords: Wheat ; Maize ; Intergeneric cross-Embryo culture ; Haploid production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four Japanese wheat varieties, three crossable and one non-crossable with Hordeum bulbosum, were pollinated with maize pollen of 5 genotypes. By the application of 2,4-dichlorophenoxyacetic acid after pollination, embryos kept developing on wheat plants until 14 days after pollination. The frequency of embryo formation was significantly different among the maize genotypes, varying from 18.0% to 31.9%, but not among the wheat varieties. By bagging spikes with flag leaves the frequency of embryo formation was increased by about 7%. Ten- to twelve-day-old embryos gave higher frequencies of plant formation (83.6%) than 14-day-old embryos(50.0%). All 6 regenerated plants investigated cytologically were found to be haploid. Twelve of the 14 colchicine-treated plants produced florets setting seeds. The overall efficiency of our procedure is considered to be higher than that reported by Laurie and Bennett (1988).
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    URL: http://dx.doi.org/10.1007/BF00274125
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    The use of irradiated pollen for differential gene transfer in wheat (Triticum aestivum) (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 103-111 
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    ISSN: 1432-2242
    Keywords: Pollen irradiation ; Wheat ; Differential gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The use of irradiated pollen to bring about limited gene transfer in wheat has been investigated. Doses of X-rays of 2Kr, 3Kr and 5Kr were used to generate M1 progeny between maternal and paternal genotypes differing in quantitative and major gene characters. Cytological studies of M1 plants revealed hybrids with widespread aneuploidy and structural rearrangements in the paternal genome. These effects resulted in phenotypic variation between M1 progeny and complex multivalent formation at meiosis. All M1 plants at the 5Kr and 3Kr doses were sterile and all but 2 plants at the 2Kr dose. Studies of the two M2 families from these plants revealed disturbances in genotype frequencies for some of the marker loci with an excess of maternal homozygotes and a deficit of paternal homozygotes. This was also reflected in a more maternal appearance for quantitative characters. These results are interpreted as showing that irradiation damage to the paternal genome in M1 plants results in the differential transmission of maternal alleles.
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    Chitinase as a possible resistance factor for higher plants (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 171-172 
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    ISSN: 1432-2242
    Keywords: Resistance ; Genetic engineering ; Yellow rust ; Wheat ; Chitinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Virulence and resistance may act on the same biochemical mechanisms. Because Erwinia-virulence on potato depends on the lysis of cell walls of the host, resistance may depend on the lysis of cell walls of the parasite. An example is given with yellow rust on wheat.
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    The Tom Thumb dwarfing gene Rht3 in wheat (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 249-256 
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    ISSN: 1432-2242
    Keywords: Wheat ; Dwarfism ; Yield ; Quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of the ‘Tom Thumb’ Rht3 dwarfing gene in wheat on plant height, yield components and grain quality in field trials of random inbred lines from two ‘Minister Dwarf’ (Rht3) x rht3 crosses are described. Limited genetic increases in Rht3 plant height, up to 70 cm, were achieved by incorporating genes from the extremely tall variety ‘April Bearded’. Amongst lines from the higher yielding cross, the dwarfing gene did not reduce grain yield but was associated with an increase in grain number of over 25%. Although Rht3 reduced grain protein content by up to 0.8%, breadmaking quality, as measured by SDS sedimentation values and baking tests, was not affected. A controlled environment experiment with near-isogenic Rht3 and rht3 lines indicated that reduced grain size in the dwarfs is a competitive response to the increase in fertility rather than a primary effect of the dwarfing gene.
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    URL: http://dx.doi.org/10.1007/BF00251155
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    The chromosomal location of malate dehydrogenase isozymes in hexaploid wheat (Triticum aestivum L.) (1983)
    Springer
    Theoretical and applied genetics 64 (1983), S. 255-258 
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    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Malate dehydrogenase ; Chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The malate dehydrogenase (E.C. no 1.1.1. 37) of Triticum aestivum L. cv. Chinese Spring, shows two activity zones. The results obtained support the hypothesis that the malate dehydrogenase isozymes of zone II are dimers composed of the six possible combinations of subunits coded by triplicate genes located in the long arms of chromosomes of the homoeologous group 1.
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    Chromosome variation in wheat plants regenerated from cultured immature embryos (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 249-255 
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    ISSN: 1432-2242
    Keywords: Wheat ; Plant regeneration ; Chromosome variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cytological study has been made of plants regenerated from cultured immature embryos of four wheat cultivars (Triticum aestivum, 2n = 6x = 42). In total, 29% of the 192 plants examined were aneuploid with a range in chromosome numbers of 38–45. Evidence of chromosome structural changes was also found. This variation occurred in regenerants of all four cultivars, but there were large differences in the proportions of aneuploids arising from individual cultures which meant that no significant differences could be demonstrated between cultivars. Chromosome abnormalities were present in plants regenerated both from embryogenic cultures and from cultures in which the origin of shoots could not be distinctly defined.
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    Wheat microspore embryogenesis during in vitro anther culture (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 439-442 
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    ISSN: 1432-2242
    Keywords: Wheat ; Multicellular pollen grains ; Embryos ; Cytology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cytological analysis of microspore embryogenesis during in vitro culture reveals a high mortality in the first week and a latency phase of about one week before the first embryogénic mitosis. Genotypic differences observed during our wheat anther culture do not seem to originate at the induction level but are linked to the different abortion rates.
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    The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 525-528 
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    ISSN: 1432-2242
    Keywords: Cryopreservation ; Embryo culture ; Haploids ; Wheat ; Rice ; Germplasm bank
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anther culture derived pollen embryos and immature zygotic embryos of wheat and rice, frozen in liquid nitrogen in the presence of dimethyl sulfoxide, sucrose and glycerol, have been revived. The retrieved cultures proliferated and/or regenerated shoots and plantlets. The prospects of the cryopreservation of embryos for the conservation and multiplication of germplasm and the possibility of the establishment of ‘Germplasm Banks’ are discussed.
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    The genetic control of grain esterases in hexaploid wheat (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 219-226 
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    ISSN: 1432-2242
    Keywords: Hexaploid genotypes ; Allelic variation ; Esterase ; Isozymes ; Isoelectric focusing ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Analysis of grain esterase isozymes in Chinese Spring aneuploid genotypes by IEF confirmed that genes on the long arms of chromosomes 3A, 3B and 3D (Est-5) control the production of 19 isozymes. Allelic variants have been found for the isozyme pattern controlled by each chromosome. Segregational data involving null alleles and complex phenotypic differences indicate that the wheat grain esterases are encoded by three compound and probably homoeoallelic loci, each capable of producing at least six different isozymes. In a sample of 138 hexaploid genotypes, seven alleles were distinguished.
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    A computer-assisted examination of the storage protein genetic variation in 841 accessions of Triticum dicoccoides (1984)
    Springer
    Theoretical and applied genetics 69 (1984), S. 79-86 
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    ISSN: 1432-2242
    Keywords: Electrophoresis ; Endosperm ; Proteins ; Emmer ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Triticum turgidum L. var. dicoccoides (wild emmer) is an important genetic resource for increasing the protein content of common wheat (Triticum aestivum L.). Many studies have shown that the presence or absence of bands in sodium dodecyl sulfate polyacrylamide (SDS-PAGE) electrophoregrams of wheat storage proteins to be of a purely genetic character. A total protein extraction and SDS-PAGE technique was used to estimate the storage protein genetic variability among 841 accessions of wild emmer collected from various ecological regions in the Middle East. In addition, a computer data bank was developed, recording the onedimension electrophoregram bands for each accession by molecular weight (MW) and relative Coomassie Blue staining intensity as determined from densitometer scans. Analyses of this information are being used to identify specific accessions for further study by two dimension electrofocusing-electrophoresis and breeding and genetic analyses. The computer-assisted analyses indicated that the greatest genetic variability occurs for proteins in the high MW region (above 70,000 MW) followed by those in the medium range (70,000 to 33,300 MW). Comparatively little variability was revealed for protein subunits of below 33,300 MW.
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    Chromosomal assignment of genes controlling salt-soluble proteins (albumins and globulins) in wheat and related species (1984)
    Springer
    Theoretical and applied genetics 69 (1984), S. 167-172 
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    ISSN: 1432-2242
    Keywords: Chromosomal location ; Genetic control ; Salt soluble proteins ; Wheat ; Barley ; Rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Salt-soluble proteins from the endosperms of wheat, barley, and rye have been separated by nonequilibrium electrofocusing x electrophoresis. Genes encoding 14 of the 25 components observed in wheat have been unambiguously assigned to 10 different chromosomes (1B, 3B, 3D, 4A, 4D, 5B, 6B, 6D, 7B, 7D) by analysis of the compensated nulli-tetrasomic series. Five more wheat proteins seem to be controlled by group 2 chromosomes. Analysis of wheat-barley and wheat-rye addition lines has led to the location of genes for 6 out of 20 barley proteins in 4 different chromosomes (1H, 3H, 4H, 6H; 1H is homoeologous to group 7 chromosomes of wheat) and of genes for 5 out of 20 rye proteins in two different chromosomes (2R, 4R). The relationship between the proteins reported here and previously characterized ones is discussed.
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    The effect of additions ofAegilops longissima chromosomes on grain protein in common wheat (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 429-435 
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    ISSN: 1432-2242
    Keywords: Aegilops longissima chromosomes ; Nitrogen fertilization ; Protein content ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of various chromosomes ofAegilops longissima when added to the common wheat cultivar ‘Chinese Spring’ was evaluated at two levels of nitrogen fertilization for absolute and relative amount of protein in the grain. All the added chromosomes ofAe. longissima increased protein percentage: protein increase by chromosomes D, C and A averaged 3.8% while that by chromosomes F, E, G and B averaged 1.7%. Addition lines F, D and C had a significantly higher protein weight per grain. On the other hand, lines A, E and G had reduced grain protein weight per grain as compared with that of ‘Chinese Spring’. Line C carries the HMW glutenin and some of the gliadin subunits ofAe. longissima. The effect of this line, however, and obviously that of the other lines on protein content was through genes controlling the level of storage protein rather than through genes that code directly for these proteins. Nitrogen fertilization affected protein content and the relative amount of the various protein fractions in a similar manner in every addition line. When high levels of nitrogen fertilization were compared to low ones, the relative amount of the HMW glutenins remained constant while that of HMW gliadins increased and that of the LMW subunits decreased. In contrast to the nitrogen effect, increase in protein content by the addition oflongissima chromosomes did not change the relative amounts of the various protein fractions.
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    Diversity of salt tolerance in a germplasm collection of wheat (Triticum spp.) (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 651-657 
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    ISSN: 1432-2242
    Keywords: Diversity analysis ; Salt tolerance ; Triticum ; Wheat ; Wheat-rye derivatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A collection (5,072 lines) of wheat germplasm was screened at the seedling stage for tolerance to salinity concentrations having electrical conductivities of 0.8 (control), 12.5, 18.75 and 25.0 dS/m. Surviving seedlings were expressed for each line as a percentage of the control value. The 442 lines with greater than 70% surviving seedlings were tested for whole-life cycle survival under each salinity condition. The data of the reactions to salinity at both the seedling stage and maturity were used to classify the collection according to: (1) country of origin (2) species and ploidy level. The data were then subjected to a diversity analysis using the Shannon-Weaver information index. Seedling stage tolerance to 12.5 dS/cm salinity was widely distributed in the collection (79% of lines), whereas only 9% were tolerant at 25.0 dS/m salinity. The seedling stage tolerance was indicative of maturity tolerance. At the seedling stage, entries from USA and Egypt showed the largest proportions of tolerant lines. In addition, USA, Mexico and Egypt entries exhibited the widest variability. Diversity among regions was greater than among countries within regions, while the diversity among species was greater than among ploidy levels. Tetraploids exceeded hexaploids and diploids in the proportion of tolerant lines and diversity. Wheat-rye derivatives showed a good potential for salt tolerance at early stages. Screening more germplasm from the arid and semi-arid regions especially from countries with salt affected soils was recommended.
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    The structure and genetic control of a new class of disulphide-linked proteins in wheat endosperm (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 79-92 
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    ISSN: 1432-2242
    Keywords: Wheat ; Disulphide-linked proteins ; LMW glutenin subunits ; Genetic Control ; 2-D electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of unreduced total protein extracts from the endosperm of hexaploid wheat revealed three high molecular weight protein bands (triplet bands) in a zone of heavy background streaking. Electrophoretic examination of 135 hexaploid cultivars showed at least five different patterns of these triplet bands. Nine durum wheat cultivars showed a single band only. Analysis of nullisomic-tetrasomic and ditelocentric lines of ‘Chinese Spring’ wheat revealed that the slowest moving band (Tri-1) of the triplet was controlled by gene(s) on chromosome arm 1DS and the fastest moving band (Tri-3) by 1AS. The band with intermediate mobility (Tri-2) was found to be a hybrid aggregate of the subunits controlled by 1DS and 1AS. Using a non-reducing/reducing form of 2-dimensional (2-D) electrophoresis, these triplet bands were shown to be heterotetramers of four subunits designated D (M.W. 58,000), δ (22,000), A (52,000) and α (23,000) where Tri-1=DδDδ, Tri-2 = DδAα and Tri-3 = AαAα. With very low concentrations of 2-mercaptoethanol (ME), the tetramers dissociated into dimeric subunit pairs (Dδ, Aα), the monomers being observed with higher concentrations of ME. The structure of these subunit pairs resembles that of the subunit pairs in the globulin storage proteins of oats and some legumes. The 2-D method employed in this study was useful also for separating low molecular weight (LMW) subunits of glutenin from the monomeric gliadins which have similar electrophoretic mobility in 1-D separation. It was shown that at least four of these LMW glutenin subunits are controlled by genes on 1DS and 1AS and at least one subunit is controlled by gene(s) on 1BS. This electrophoretic separation method has proven useful in understanding the aggregation behaviour of the seed proteins of wheat.
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    Organelle genome stability in anther-derived doubled haploids of wheat (Triticum aestivum L., cv. ‘Moisson’) (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 320-324 
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    ISSN: 1432-2242
    Keywords: Wheat ; Anther-derived doubled haploids ; Chloroplast DNA ; Mitochondrial DNA ; Restriction patterns
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chloroplast and mitochondrial compartments of a parental line of wheat (Triticum aestivum L., cv. ‘Moisson’) and its anther-derived doubled haploid lines have been analyzed and compared on the basis of their DNA restriction patterns. The results obtained show that no noticeable difference can be detected between doubled haploid lines and parental line at the level of ctDNA and mtDNA organization. It may be concluded that in vitro culture by itself does not systematically generate a cytoplasmic variation in germ cells.
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    URL: http://dx.doi.org/10.1007/BF00252074
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    Genetical analysis of chromosome 5A of wheat and its influence on important agronomic characters (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 518-526 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Vernalisation genes ; Agronomic characters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chromosome 5A of bread wheat, Triticum aestivum carries the major gene, Vrnl, which is one of the main determinants of the winter/spring growth habit polymorphism in this species. Genetical analysis of this chromosome has been carried out using single-chromosome recombinant lines to establish the pleiotropic effects of this locus and two other major genes, q determining ear morphology and bl determining the presence of awns, on important agronomic characters. The three major genes were located on the long arm of chromosome 5A with a gene order of: centromere -bl-q-Vrnl. Analysis of quantitative characters from a winter sowing revealed pleiotropic effects of Vrnl or the effects of closely linked loci on the characters plant height, tiller number and spikelet number. However effects on ear emergence time were not associated with Vrnl but with q as were effects on spikelet number and ear length. In addition a locus determining yield/plant was located between Vrnl and q. Independant loci determining height and ear length were apparent on the short arm of chromosome 5A. From a spring sowing, however, there was a large pleiotropic effect of Vrnl on ear emergence time, as well as the effects previously detected. In addition, associated with q were effects on plant height and grain size which were not expressed from the winter sowing.
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    URL: http://dx.doi.org/10.1007/BF00251199
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    Genetic control of heading date and spikelet number in common wheat (T. aestivum L.) line ‘Noa’ (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 105-107 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; T. aestivum ; Heading date ; Spikelet number ; Phenology ; Day-length insensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The phenology and build-up of spikelet number under 10 h day-length were studied in five wheat lines: the multispikelet line ‘Noa’, the regular line ‘Mara’, the F1 hybrid between them and monosomics 2D of ‘Mara’ and of this hybrid (lacking the 2D chromosome of ‘Mara’). ‘Noa’ had a longer spike development phase, a higher initial number of spikelet primordia and a slower rate of spikelet production than ‘Mara’. The F1 hybrid was similar to ‘Noa’ in its high initial number of spikelets and to ‘Mara’ in its high rate of spikelet production. This hybrid had a shorter spikelet phase than both parents. Deletion of one dose of the ‘Mara’ 2D chromosome from either ‘Mara’ or the F1 hybrid caused a reduction in the rate of spikelet production and an increase in the duration of the spikelet phase. These effects were due to the reduced dosage of the 2D chromosome. However, in the F1 hybrid this deletion also caused an increase in the spike development phase — an indication that ‘Noa’ carries on its 2D chromosome a recessive gene for late heading date which acts on the spike development phase. This gene of ‘Noa’ is independent of the day-length sensitive gene ppd, and is different from ‘Noa’s dominant gene for large initial number of spikelets.
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    URL: http://dx.doi.org/10.1007/BF00261463
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    Wheat specific repetitive DNA sequences — construction and characterization of four different genomic clones (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 207-210 
    Details
    ISSN: 1432-2242
    Keywords: Repetitive DNA ; Wheat ; Rye ; Relic DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The construction and molecular analysis of four recombinant clones — pTa1, pTa2, pTa7, and pTa8 — is described. The four clones contain different highly repeated sequences of genomic DNA from Triticum aestivum variety ‘Chinese Spring’. The wheat specificity has been determined by colony and dot blot hybridization in comparison with total rye DNA (Secale cereale variety ‘Petka’). The four clones with a variable degree of specificity were compared by sequence analysis after the recloning of wheat DNA inserts into M13 mp8. Within the sequencing data a tendency can be observed that those repeated sequences which show the highest degree of species specificity contain a significantly increased amount of GC residues.
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    URL: http://dx.doi.org/10.1007/BF00266993
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    The genetic control of grain esterases in hexaploid wheat (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 219-225 
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    ISSN: 1432-2242
    Keywords: Esterase isozymes ; Isoelectric focusing ; Wheat ; Rye ; Barley ; Aegilops ; Agropyron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison of EST-5 grain esterase phenotypes from wheat-alien amphiploid, addition and substitution genotypes, resolved by flat-bed isoelectric focusing identified homoeologous Est-5 loci on chromosome 3H of Hordeum vulgare, 3Hch of H. chilense, 3Sb of Aegilops bicornis, 3S1 of Ae. sharonensis and Ae. longissima and 6R of Secale cereale and 6Rm of S. montanum. The Est-5 genes in alien species provide evidence for chromosome homoeology with wheat.
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    URL: http://dx.doi.org/10.1007/BF00266995
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    Quantitative variation in the kernel proteins among 841 accessions of Triticum dicoccoides estimated by SDS-PAGE (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 296-301 
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    ISSN: 1432-2242
    Keywords: Electrophoresis ; Endosperm ; Proteins ; Wild emmer ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relative proportion and amount of proteins in five defined molecular weight (MW) regions (A1=above 71,000=71K, A2=71K−49K, A3=49K−31K, A4=31K−20K, A5=20K and less) were estimated by densitometric analyses of the amount of dye bound by kernel proteins (Fullington et al. 1980) of Triticum dicoccoides SDS-PAGE gels. These MW regions roughly correspond to the wheat protein solubility classes (Cole et al. 1981; Fullington et al. 1983). One purpose of the study was to select accessions whose seed proteins bind relatively high amounts of dye in the glutenin and albumin globulin regions. These accessions will be used for further in-depth studies as possible candidate donors of genes to improve the baking and nutritional quality of wheat. Marked differences in the quantitative relationships were found among the proteins in the five MW regions. Coefficients of variation (CV's) for the highest peak (i.e., most abundant protein) MW in different protein MW regions were similar for A1, A2 and A3, at 11.4, 11.7, and 11.1%, respectively, but only 4.1 for A4, and 10.6% for region A5. The CV for the highest peak MW overall was 29.8. Accession BP0649, for example, had over 44% of its protein in region A5, whereas BP0566 (lowest among the top 10%) had only 21.4% of its protein in that region. Over 37% of the proteins of accessions BP0649 and 0001 to 0005 was in region A5. At least 84 accessions with the highest amount of protein in region A5, and 13 accessions with more protein in region A1 than Chinese Spring may merit further evaluation as possible protein gene donors. High amounts of protein in A1 may be of importance in bread-baking quality, and in A4 and A5 for high lysine wheat. Accessions in both extremes were selected to test these hypotheses. All accessions are now or will be available in the USDA Wheat Collection.
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    URL: http://dx.doi.org/10.1007/BF00288564
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    The effect of the crossability loci Kr1 and Kr2 on fertilization frequency in hexaploid wheat x maize crosses (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 403-409 
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    ISSN: 1432-2242
    Keywords: Wheat ; Maize ; Crossability genes ; Chromosome elimination ; Haploids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Dominant alleles of the Kr1 and Kr2 genes reduce the crossability of hexaploid wheat with many alien species, including rye and Hordeum bulbosum, with Kr1 having the greater effect. However, a cytological study of wheat ovaries fixed 48 h after pollination showed that the wheat genotypes ‘Highbury’ (kr1, Kr2) and ‘Chinese Spring (Hope 5B)’ (kr1, kr2) were crossable with ‘Seneca 60’ maize, fertilization occurring in 14.4 and 30.7% of embryo sacs respectively. The latter figure was similar to the 29.7% fertilization found in ‘Chinese Spring’ (kr1, kr2). Most embryo sacs in which fertilization occurred contained an embryo but lacked an endosperm and where an endosperm was formed it was usually highly aberrant. All three wheat x maize combinations were karyotypically unstable and rapidly eliminated maize chromosomes to produce haploid wheat embryos.
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    URL: http://dx.doi.org/10.1007/BF00262508
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    Nor loci analysis in progenies of plants regenerated from the scutellar callus of bread-wheat (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 827-831 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Ribosomal DNA intergenic spacer ; Scutellum ; Tissue culture ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Progenies of plants regenerated from scutellar callus of bread wheat (Triticum aestivum L.) were analysed for the organization of the intergenic spacer of the rRNA genes, located at the sites of the nucleolar organizer region (Nor loci). Sexual progenies derived from the regenerated plants of three wheat cultivars were subjected to this analysis. The respective DNAs were digested with the restriction endonuclease TaqI, and probed with a specific rDNA fragment by Southern blothybridization. The intergenic rDNA spacer could thus be characterized for each of the three cultivars. Thirty-eight progeny plants of the cultivars ‘Chinese Spring’ and ‘Miriam’ were found to be stable in their organisation of the Nor loci: no changes relative to the Nor of control plants from these cultivars were revealed. On the other hand, three progeny plants of ‘ND7532’ showed reduction in the number of the rDNA spacers. Since no variability in the Nor loci could be revealed among control ‘ND7532’ plants, this seems to indicate that the changes in the progeny of regenerated plants resulted from the in vitro culture of the scutellar callus. Grain glutenin and gliadin profiles of sexual progenyplants derived from scutellar calli of ‘Chinese Spring’, of ‘Miriam’ as well as of ‘ND7532’ were identical to the respective control plants of these cultivars, indicating low (or no) somaclonal variation in these grain proteins in the analyzed plants.
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    URL: http://dx.doi.org/10.1007/BF00289386
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    Genetic control of dipeptidase in the Triticeae (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 387-390 
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    ISSN: 1432-2242
    Keywords: Isozymes ; Dipeptidase ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Isoelectric focusing has been employed to elucidate the genetic control of a series of dipeptidase isozymes in wheat and its relatives. The phenotype of wheat shows four bands, three of which are shown by aneuploid analysis to be controlled by the loci Dip-A1, Dip-B1 and Dip-D1 on chromosome arms 6AL, 6BL and 6DL, respectively. Varietal polymorphism for Dip-A1 and Dip-B1 was observed. Different homoeoloci were found in barley, Haynaldia villosa and Agropyron junceum.
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    URL: http://dx.doi.org/10.1007/BF00274722
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    Identification of new mitochondrial genome organizations in wheat plants regenerated from somatic tissue cultures (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 169-175 
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    ISSN: 1432-2242
    Keywords: Mitochondrial DNA ; Chondriome variability ; In vitro culture ; Plant regeneration ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants have been regenerated from short-and long-term in vitro somatic tissue cultures made from immature embryos of the hexaploid wheat cultivar “Chinese Spring”. The mitochondrial genome organization of each regenerated plantlet was studied, after one selfing, by probing Sal I-restricted total DNA with cloned Sal I fragments of wheat mitochondrial DNA derived from a segment of the genome, which displays marked structural changes in response to in vitro culture. Short-term in vitro cultures give rise to regenerated plants whose mitochondrial genome organization is either close to that of the parental cultivar or to that of embryogenic callus cultures, except for a single plant which has an organization resembling that of short-term non-embryogenic cultures. In contrast, all but one of the plants regenerated from long-term cultures exhibited a mitochondrial genome organization similar to that of long-term nonembryogenic cultures. In addition, extra labelled bands were detected in some of the regenerated plants with two of the probes used. These results emphasize the importance of the duration of the in vitro step preceding the regeneration process: the longer it is, the higher the probability is of obtaining mitochondrial DNA variability in regenerated plants. Furthermore, since increasing the duration of the in vitro stetp results in the production of regenerated plants with a mitochondrial genome organization resembling that of non-embryogenic tissue cultures, the question is thus raised as to whether regeneration from long-term cultures is suitable for use in plant breeding.
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    URL: http://dx.doi.org/10.1007/BF00266182
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    Biochemical and cytological characterization of wheat/Aegilops ventricosa addition and transfer lines carrying chromosome 4MV (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 184-188 
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    ISSN: 1432-2242
    Keywords: Wheat ; Aegilops ventricosa ; Powdery mildew resistance ; Biochemical markers ; Addition and transfer lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gene encoding a variant of alcohol dehydrogenase, Adh-μ, has been found to be associated with the chromosome of the Mv genome which is present in type 9 wheat/Aegilops ventricosa addition line, to which the genes for protein CM-4 and for a phosphatase variant, Aph-v, had been previously assigned. Transfer line H-93-33, which has 42 chromosomes and has been derived from the cross (Triticum turgidum x Ae. ventricosa) x T. aestivum, carries genes encoding all three biochemical markers. Linkage between these genes has been demonstrated by analysis of individual kernels of the F2 (H-93-33 x T. aestivum cv. “Almatense” H-10-15). A study of the hybrids of line H-93-33 with T. aestivum H-10-15 and with the 4DS ditelosomic line has confirmed that, as suspected, the linkage group corresponds to chromosome 4Mv from Ae. ventricosa. Additionally, it has been found that the previously reported resistance of line H-93-33 to powdery mildew (Erysiphe graminis) is also linked to the biochemical markers; this indicates that either the gene responsible for it is different from that in lines H-93-8 and H-93-35, or that a translocation between two different Mv chromosomes has occurred in line H-93-33.
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    URL: http://dx.doi.org/10.1007/BF00266184
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    Genetic variability and advance under four selection procedures in wheat pedigree breeding programme (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 516-520 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Single seed descent (SSD) ; Single plant selection (SPS) ; Bulk population (BP) ; Mechanical mass selection (MMS)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four methods of generation advance (SPS, SSD, BP and MMS) were compared in F3 and F4 generations. In the F3 generation, the SPS and SSD methods of generation advance proved superior to the BP and MMS methods for grain yield per plant and for at least one of the yield component traits. The F3 SSD population did not differ significantly from the F3 SPS for any of the traits. However, the F3 SSD population retained more range and cv for different traits than with other methods of generation advance. F4 progenies derived from F3 SSD population were significantly superior for grain yield than lines derived from the other three F3 populations. The MMS method of generation advance proved useful for increasing the 1,000-grain weight for which initial selection was made.
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    URL: http://dx.doi.org/10.1007/BF00274272
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    Comparative performance of bread wheat and hexaploid triticale cytoplasms (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 685-688 
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    ISSN: 1432-2242
    Keywords: Wheat ; Triticale ; Cytoplasmic effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirteen wheat-like advanced-generation triticale x wheat derivatives, having tetraploid wheat cytoplasm from triticale, were reciprocally crossed with three improved bread wheats, and the resulting F1s were evaluated for determining the comparative performance of the bread wheat and triticale cytoplasms for different traits. Significant reciprocal differences in the mean performance were observed for days to heading, days to maturity, spikes/plant, flag-leaf area, peduncle length, plant height, spike length, grains/spike, 1,000-grain weight, grain yield and grain protein content, and most of them were in favour of hexaploid wheat cytoplasm. However, this superiority of the hexaploid cytoplasm was not universal for a particular trait, implying that the differences in the performance of the evaluated reciprocal crosses depended not solely on the cytoplasmic background, but also on the interplay of the specific genotype with the cytoplasm.
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    Is Nor region variability in wheat invariably caused by tissue culture? (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 809-814 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Ribosomal DNA ; Tissue culture ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a previous study we observed extensive Nor region variability in tissue-culture derived plants of only one out of three tested wheat cultivars. This finding prompted us to further question whether or not this variability was invariably caused by in vitro culture. In the present study, the upper halves of spikes from four source plants of the inbred cultivar ‘ND7532’ were removed 12 days after anthesis. The immature embryos from these halves were cultured and regenerated into plants. The lower halves of the same spikes were retained on the plants to obtain mature caryopses. DNA was extracted from seedlings, cut with TaqI endonuclease, run on agarose gels, and the respective Southern blots were probed with the plasmid pTA71 to reveal the Nor region patterns. The sexual progeny of regenerants from three out of four source plants derived from the immature embryos provided Nor region patterns which were exactly identical to the patterns obtained from seedlings which germinated from the caryopses matured on the respective source spikes. The regenerants from the fourth source plant provided variable Nor region patterns. Analyses of the Nor region patterns of 21 individual seedlings germinated from caryopses of this source plant showed that 18 had a three-fragment pattern (consisting of 3.0, 2.7 and 1.9 kb fragments) while three seedlings lacked one (2.7 of 1.9 kb) fragment. Furthermore, the next sexual progeny of the regenerants which had a three-fragment pattern further segregated into three- and two-fragment patterns.These results, in conjunction with previous reports on Nor region variability among tissue-culture derived plants, suggest that this variability is not invariably related to in vitro culture.
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    URL: http://dx.doi.org/10.1007/BF00268331
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    Use of tissue culture to bypass wheat hybrid necrosis (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 57-60 
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    ISSN: 1432-2242
    Keywords: Hybrid necrosis ; Wheat ; Immature embryoculture ; Young ear culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hybrid necrosis in wheat is a barrier to gene transfer in wheat breeding practice. It is based on two complementary genes, Ne1 and Ne2. Recovery mutants (Re1, Re2 and Re3) which can grow to maturity were recovered from immature embryo cultures of necrotic hybrids between T. aestivum and T. durum. Cytological observation demonstrated that Re1 had 34 chromosomes instead of 35. This indicated that one of the chromosomes carrying the Ne genes was lost. Genetic study suggested that for Re1, the lost chromosome was chromosome 5B of the durum parental line. Re mutants are male sterile but can be maintained through a young ear culture method. Re mutants could be successfully pollinated by either parental line and the BC1 progeny is partially fertile. Re mutants were repeatedly induced in about 1% of the regenerated plants from immature embryo culture. This technique provides a practical way to bypass hybrid necrosis.
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    Inheritance of somatic embryogenesis and organ regeneration from immature embryo cultures of winter wheat (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 137-142 
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    ISSN: 1432-2242
    Keywords: Wheat ; Diallel analysis ; Somatic embryogenesis ; Plant regeneration ; In vitro culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Diallel analyses of F1 and reciprocal crosses among five winter wheat lines show that additive, non-additive, and cytoplasmic genetic effects were significant in the genetic control of somatic embryogenesis, shoot, and root induction frequencies as well as in numbers of somatic embryos, shoots, and roots. However, additive genetic effect appears to be most important since, in most cases a larger portion of the cross variation was accounted for by general combining ability. The results suggest that somatic embryogenesis and organ regeneration in winter wheat can be improved through genetic manipulation. Due to the presence of maternal effects, it may be critical to use a suitable genotype as a female parent in a selection program.
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    URL: http://dx.doi.org/10.1007/BF00299767
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    Control of tissue culture response in wheat (Triticum aestivum L.) (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 783-787 
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    ISSN: 1432-2242
    Keywords: Tissue culture ; Callus ; Wheat ; Ditelosomics ; Nullisomic-tetrasomics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ability of immature embryos of wheat (Triticum aestivum L.) to respond to tissue culture has been shown to involve the group 2 chromosomes. The available group 2 ditelosomic and nullisomic-tetrasomic lines of ‘Chinese Spring’ wheat were used to determine the chromosome arm location and chromosome dosage effect associated with the expression of tissue culture response (TCR). Significant differences were found between the aneuploid lines and the euploid control for the expression of both regenerable callus formation and callus growth rate. A model is proposed suggesting that a major TCR gene is located on 2DL and that 2AL and 2BS possess minor TCR genes. Furthermore, a major regulatory gene controlling the expression of TCR genes may be located on chromosome 2BL.
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    Evidence for cytoplasmic control of in vitro microspore embryogenesis in the anther culture of wheat (Triticum aestivum L.) (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 867-872 
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    ISSN: 1432-2242
    Keywords: Wheat ; Cytoplasm ; Anther culture ; Microspore embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anthers were cultured from two sets of seven lines of hexaploid wheat (Triticum aestivum L.) with different cytoplasms, the euplasmic nucleus donors, ‘Siete Cerros 66’ and ‘Penjamo 62’, as well as their six alloplasmic lines derived from wild relative species of the genera Triticum and Aegilops. Significant cytoplasmic and nuclear effects but no cytoplasmic-nuclear interaction were found for embryogenic anther response, with the best performance of ‘Penjamo 62’ in Ae. kotschyi cytoplasm. Plant regeneration was not affected significantly by the cytoplasmic background of the lines cultured. The possible genetic implications of the observed cytoplasmic and nuclear influences on the in vitro haploid induction of wheat are discussed.
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    The relationship between the Rht 1 and Rht 2 dwarfing genes and grain weight in Triticum aestivum L. spring wheat (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 153-157 
    Details
    ISSN: 1432-2242
    Keywords: Inheritance ; Culm-length ; GA insensitivity ; Grain weight ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The study was carried out in the first year on samples of random F5 lines, uniform in height and in heading date, of three crosses between semi dwarf spring wheat cultivars (Triticum aestivum L.), differing in grain weight and in their Rht gene. In the second year only the progenies of the early heading F5 lines were studied. All the material was grown in the absence of lodging. The culm-length genotypes of the different lines were identified by test crosses and by a seedling GA response test. No differences in grain weight were found between the two semi dwarf genotypes (Rht 1Rht1 rht 2rht2 and rht 1rht1 Rht 2Rht2). The tall genotype (rht 1rht1 rht 2rht2) was significantly higher in grain weight than the two semi dwarf genotyes and the grain weight of these genotypes exceeded markedly the grain weight of the dwarf genotype (Rht 1Rht1 Rht 2Rht2). These genotypic effects were independent of differences in plant height, heading date or number of grains per spike.
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    URL: http://dx.doi.org/10.1007/BF00265191
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    Inheritance of glutenin subunits in F1 seeds of reciprocal crosses between European hexaploid wheat cultivars (1983)
    Springer
    Theoretical and applied genetics 64 (1983), S. 103-107 
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    ISSN: 1432-2242
    Keywords: Glutenin ; Wheat ; Co-dominance ; Bread-making
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ten pairs of reciprocal crosses have been made between wheat cultivars which show differences in their glutenin subunit compositions. The F1 seed glutenin subunit composition was studied by means of polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS). The results indicate that all the high molecular weight (HMW) and medium molecular weight (MMW) subunits (from 133,000 to 65,000 daltons) are transmitted to the F1 seed generation from the parental cultivars. In accordance with the triploid nature of the heterozygous endosperm (3n) and with the maternal and paternal gene dosage ratio (2∶1) in the endosperm itself, a significant effect of maternal parent is registered when comparing pairs of reciprocal seeds. Genes coding for the glutenin subunits are expressed whatever their doses are (one, two, or three) in the hybrid endosperm; thus the glutenin subunits inheritance is consistent with the co-dominant type. For one pair of the reciprocal crosses, two MMW parental bands (MW: 71,000 and 66,000) seemed absent in the F1 seed patterns while a new band with an intermediate, apparent MW (68,000) appears. This phenomenon was observed when the glutenins analyzed by electrophoresis were previously separated from other endosperm proteins, and not when they were directly extracted from the ground seed. We assume that the extraction can cause interactions between moieties attached to the subunits and lead to the formation of a complex having an intermediate electrophoretic mobility.
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    URL: http://dx.doi.org/10.1007/BF00272713
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    Genetic studies of the crossability of hexaploid wheat with rye and Hordeum bulbosum (1983)
    Springer
    Theoretical and applied genetics 64 (1983), S. 303-307 
    Details
    ISSN: 1432-2242
    Keywords: Crossability ; Wheat ; Rye ; Hordeum bulbosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Crosses of wheat (Triticum aestivum L.) Group 5 chromosome substitution lines from three different cultivar backgrounds indicate that there may be multiple alleles for reduced crossability with rye (Secale cereale L.) and H. bulbosum on both 5A and 5B chromosomes. No reduction in seed set was observed from any of the 5D substitution lines. It was found that the Kr1 locus on chromosome 5B had a more pronounced affect on both rye and H. bulbosum crossability than the Kr2 locus on chromosome 5A and that the effects of both loci were cumulative. The Kr alleles affected both rye and H. bulbosum crossability in a linear, or additive, fashion. The Kr 2 allele was shown to have little effect on H. bulbosum crossability compared to a significant effect on rye crossability. Tests with tetrasomic and nulli-tetrasomic lines of ‘Chinese Spring’ indicated that the kr allele is ‘null’ or inactive in promoting crossability while the Kr allele is active in reducing crossability to both rye and H. bulbosum. Thus, extra doses of the kr allele do not increase rye or H. bulbosum crossability in the presence of the corresponding Kr allele.
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    URL: http://dx.doi.org/10.1007/BF00274168
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    Genetic control of α-Amylase production in wheat (1983)
    Springer
    Theoretical and applied genetics 64 (1983), S. 309-316 
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    ISSN: 1432-2242
    Keywords: Wheat ; α-Amylase ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An analysis of the α-amylase isozymes in GA-treated endosperm of wheat nullisomic-tetrasomics shows that there is more variation at the α-Amy-1 and α-Amy-2 homoeoallelic loci than was previously thought. Among the 16 isozymes produced by genes on the group 7 chromosomes, most could be definitely established as products of a single homoeoallele. Inter-varietal allelic differences would be expected at such loci and clear variation was found in isozymes produced by chromosomes 6B and 7B. The latter allele, α-Amy-B2b carried by the variety ‘Hope’, was used to locate the enzyme structural gene within chromosome 7B relative to the centromere and five other gene markers. The nature of the α-Amy-B2b phenotype and the rare non-parental isozyme patterns found among the recombinant lines indicates that the locus is large and compound, probably involving some degree of intra-locus gene duplication.
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    URL: http://dx.doi.org/10.1007/BF00274170
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    Characterisation of high molecular weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal localisation of their controlling genes (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 29-37 
    Details
    ISSN: 1432-2242
    Keywords: Gliadin ; Glutenin ; Tticum ; Wheat ; Endosperm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gliadins, here defined as those proteins of defatted wheat endosperm which dissolve in 70% (v/v) ethanol at room temperature, were fractionated by gel filtration using Sephadex G-100. The protein which eluted with the void volume of the column, often described as high-molecular-weight (HMW) gliadin, was fractionated by the two different, two dimensional gel electrophoresis procedures of O'Farrell (1975) and O'Farrell et al. (1977). The next two fractions to elute from the gel column, ω-gliadin and α-, β-, γ-gliadin, were analysed similarly. The subunits of HMW gliadin and the classical (i.e. non-aggregated) gliadins map at distinctive positions on the electrophoregrams, the majority of the HMW gliadin subunits being more basic and having a slightly slower electrophoretic mobility than the α-, β-, γ-gliadins. These experiments demonstrate that those gliadins which aggregate to form HMW gliadin are distinct molecular entities and thus coded by different genes to those gliadins which do not aggregate. Glutenin, here prepared by a modification of the pH 6.4 precipitation procedure of Orth and Bushuk (1973), was also analysed by two-dimensional electrophoresis. The low-molecular-weight subunits were found to correspond exactly with the HMW gliadin subunits. Using the nullisomic-tetrasomic lines and the ditelocentric lines of ‘Chinese Spring’, the genes controlling the synthesis of all the major HMW gliadin subunits were shown to be located on the short arms of chromosomes 1A, 1B and 1D, as are the genes coding for the ω-gliadins and the majority of the γ-gliadins.
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    URL: http://dx.doi.org/10.1007/BF00281844
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    Evidence for maternal effect in the inheritance of grain protein in crosses between cultivated and wild tetraploid wheats (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 521-524 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Triticum ; Grain protein ; Grain weight ; Maternal effect ; Cytoplasmic effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Reciprocal crosses were made between cultivated wheat (Triticum turgidum var. ‘durum’) and a high-protein line of wild tetraploid wheat (T. turgidum var. ‘dicoccoides’). F1 grains (on maternal spikes) were very similar to the selfed grains on the maternal parent in protein percentage, weight and protein content. These traits were also analyzed in F3 grains developed on F2 spikes of segregating populations derived from reciprocal crosses between the same cultivated parent and another high-protein line of var. ‘dicoccoides’. No significant differences in the mean values of these traits were found between the reciprocal crosses, indicating no cytoplasmic effect. It has been concluded that these grain characteristics are largely determined by the maternal plant.
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    URL: http://dx.doi.org/10.1007/BF00264897
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    Heritability of grain yield, plant height and test weight of a population of winter wheat adapted to Southwestern Ontario (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 21-23 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Heritability ; Yield ; Height
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Grain yield, plant height and test weight were studied in a population of winter wheat (Triticum aestivum L.). The population consisted of F2 bulk populations of 42 crosses among 11 genotypes adapted to S. W. Ontario. Heritabilities were: 0.30±0.32 for yield, 0.77±0.15 for height and 0.98±0.08 for test weight. Predicted genetic gain with 10% selection intensity was 0.15 t/ha for yield, 10.1 cm for height and 3.00 kg/hl for test weight. The low heritability for yield indicates that effective selection would require pedigree information and progeny tests, while the high heritabilities for height and test weight indicate that selection for these traits using single plots would be appropriate.
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    URL: http://dx.doi.org/10.1007/BF00252304
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    Intrachromosomal mapping of crossability genes in wheat (Triticum aestivum) (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 309-314 
    Details
    ISSN: 1432-2242
    Keywords: Crossability genes ; Wheat ; Intrachromosomal mapping ; Rye ; Hordeum bulbosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intrachromosomal mapping studies were used to locate the positions of the genes Kr1 and Kr2, which control the crossability of wheat with Hordeum bulbosum, on chromosomes 5B and 5A, respectively. The location of Kr1 was established using the telocentric mapping technique and found to be on the long arm of chromosome 5B, distal to the centromere with a mean recombination frequency of 44.8±3.28%. Kr2 was located on the long arm of chromosome 5A by linkage with the major gene markers Vrn1, controlling vernalization requirement, and q, controlling ear morphology. Kr2 is closely linked to Vrn1, with a mean recombination frequency of 4.8±4.66%, and is distal to q with a mean recombination frequency of 38.1±10.60%. The similar locations of Kr1 and Kr2 on homoeologous chromosomes suggest that these two loci are homoeoallelic. Significant correlations between Hordeum bulbosum and rye crossability confirmed that Kr1 and Kr2 control the crossability of wheat with both species.
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    URL: http://dx.doi.org/10.1007/BF00304917
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    The effect of specific chromosome and cytoplasm substitutions on the tissue culture response of wheat (Triticum aestivum) callus (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 797-800 
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    ISSN: 1432-2242
    Keywords: Wheat ; Callus ; Regeneration ; hromosomes ; Cytoplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calli were initiated from immature embryos of four lines of hexaploid wheat (Triticum aestivum L. em. Thell), the euplasmic nuclear donor ‘Chinese Spring’, ‘Chinese Spring’ in which both 4B chromosomes were substituted by those of the variety ‘Cappelle-Desprez’ and two alloplasmic lines in which these nuclei were substituted into the cytoplasm of Aegilops ovata. The calli were found to differ in their initia growth rates and their ability to organise shoot primordia and regenerate shoots. The ‘Cappelle’ 4B chromosomes had a very significant effect on all these characters. The potential for modelling genotypes for improved tissue culture characteristics is discussed.
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    URL: http://dx.doi.org/10.1007/BF00276420
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    Cytoplasmic effects on the tissue culture response of wheat (Triticum aestivum) callus (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 70-75 
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    ISSN: 1432-2242
    Keywords: Cytoplasm ; Wheat ; Tissue culture ; Callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calli were initiated from immature embryos of eight lines of hexaploid wheat (Triticum aestivum L. em. Thell) with different cytoplasms, the euplasmic nuclear donor ‘Chinese Spring’ and seven alloplasmic lines derived from wild relative species of the genera Triticum and Aegilops. The calli were found to differ in their initial growth rates, their sensitivity to 2,4-D and their ability to organise shoot primordia, demonstrating that the cytoplasm can significantly affect the behaviour of tissues in culture. The potential for improving the responses of tissues in culture by cytoplasmic changes is noted.
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    URL: http://dx.doi.org/10.1007/BF00261457
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    Genetic transfer of resistance to powdery mildew and of an associated biochemical marker from Aegilops ventricosa to hexaploid wheat (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 605-608 
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    ISSN: 1432-2242
    Keywords: Wheat ; Aegilops ventricosa ; Erysiphe graminis f.sp. tritici ; Powdery mildew resistance ; Protein U-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Resistance to powdery mildew, caused by the fungus Erysiphe graminis f.sp. tritici, has been transferred from Aegilops ventricosa (genomes DvMv) to hexaploid wheat (Triticum aestivum, ABD). In two transfer lines, H-93-8 and H-93-35, the resistance gene was linked to a gene encoding protein U-1, whereas one line, H-93-33, was resistant but lacked the molecular marker, and another line, H-93-1, was susceptible but carried the gene for U-1, indicating that the original Mv chromosome from Ae. ventricosa, carrying the two genes, had undergone recombination with a wheat chromosome in the last two lines.
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    URL: http://dx.doi.org/10.1007/BF00289201
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    Chromosome variation in dividing protoplasts and cell suspensions of wheat (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 140-146 
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    ISSN: 1432-2242
    Keywords: Wheat ; Protoplasts ; Cell suspensions ; Aneuploidy ; Structural chromosome variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytology of bread wheat (Triticum aestivum) suspension lines, recycled lines (selected for high division frequency) and their dividing protoplasts, have been examined. Extensive numerical and structural chromosome variation was present in all the lines. The most frequently observed chromosome numbers were around 2n=32, indicating that considerable chromosome loss from the normal wheat complement (2n=6x=42) had occurred during selection of the lines. Chromosome aberrations also indicated loss of chromosome arms and chromosome segments. The implications of this variation for studies on transformation and for the potential regeneration of whole plants from protoplasts of bread wheat are discussed.
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    URL: http://dx.doi.org/10.1007/BF00290096
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    Genetic variability for heat shock proteins in common wheat (1987)
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    Theoretical and applied genetics 74 (1987), S. 209-213 
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    ISSN: 1432-2242
    Keywords: Genetic variability ; Heat shock proteins ; Wheat ; Two-dimensional electrophoresis ; Thermal tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The response of the common wheat line ‘Chinese Spring’ to heat shocks of different time lengths was studied by the two-dimensional (2D) electrophoresis of denatured proteins. After a heat shock of 5 h, 33 heat shock proteins (HSPs) accumulated in an amount sufficient to be revealed by silver stain. Two other wheat lines (‘Moisson’ and ‘Selkirk’) were then submitted to a heat shock of 5 h, and the responses of the 3 lines were compared: of a total of 35 HSPs, 13 (37.1%) were quantitatively or qualitatively variable. This variability concerns low-molecular-weight and high-molecular-weight HSPs. The three genotypes showed thermal tolerance but ‘Chinese Spring's’ response to heat treatments was slightly different from those of the other two lines The possibility of a relationship between HSP patterns and thermal sensitivity is discussed.
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    URL: http://dx.doi.org/10.1007/BF00289970
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    Interaction between genes controlling a new group of glutenin subunits in bread wheat (1987)
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    Theoretical and applied genetics 74 (1987), S. 459-465 
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    ISSN: 1432-2242
    Keywords: Wheat ; Glutenins ; Genetic interaction ; Linkage mapping ; SDS-PAGE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary One-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of reduced total protein extracts from the endosperm of hexaploid wheat revealed a new set of faintly-stained bands, having slower electrophoretic mobility than the high-molecular-weight (HMW) glutenin subunits. These new bands have been termed the E group of glutenin subunits. Analysis of aneuploid stocks of Chinese Spring wheat has shown that three of the E bands, in order of increasing electrophoretic mobility, are controlled by genes on the short arms of chromosomes 1B, 1A and 1D, respectively. The E bands are expressed only in the presence of the long arm of chromosome 1B indicating an interaction between two or more genes involved in their production in wheat endosperm. The gene on the short arm of chromosome 1D controlling an E subunit recombined freely with Tri-D1 and the centromere but not at all with Gli-D1, indicating additional complexity at the Gli-DI locus in wheat.
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    URL: http://dx.doi.org/10.1007/BF00289821
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    Monosomic analysis of heading date and spikelet number in the common wheat (Triticum aestivum L.) multispikelet line ‘Noa’ (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 487-492 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; T. aestivum ; Heading date ; Spikelet number ; Monosomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic analysis of heading date and spikelet number was carried out in the common wheat (Triticum aestivum L.) multispikelet line ‘Noa’, by using the monosomic series of the regular line ‘Mara’. ‘Noa's’ high number of spikelets was found to be controlled by a recessive major gene on chromosome 2D; a slight reduction in spikelet number was induced by another recessive gene on ‘Noa's’ 7A chromosome. ’Noa's’ late heading date was found to be controlled by two recessive genes, located on chromosome 2D (a major effect) and 6B (a minor effect). The nature of the genes located on ’Noa's’ 2D chromosome and the relationship between spikelet number and heading date are discussed.
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    URL: http://dx.doi.org/10.1007/BF00289827
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    In vitro selection of barley and wheat for resistance against Helminthosporium sativum (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 841-845 
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    ISSN: 1432-2242
    Keywords: In vitro selection ; Helminthosporium sativum ; Disease resistance ; Protein isozymes ; Barley ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calli derived from immature embryos of barley and wheat genotypes were screened for their resistance to purified culture filtrate produced by the fungus Helminthosporium sativum P.K. and B. Two selection methods were used: a continuous method in which four cycles of selection were performed one after another on toxic medium and a discontinuous method in which a pause on non-toxic medium was given after the second or third cycle of selection. The latter was superior as it allowed the calli to regain their regeneration ability. About 3,000 calli from two barley genotypes and 2,000 from two wheat genotypes were used for selection. The selection with the pathotoxins resulted in 6% to 17% surviving calli. Toxin tolerant callus lines of barley were characterised by protein isozymes. Zymograms showed one more isozyme than with the unselected sensitive callus. Barley and wheat plants have been regenerated from callus lines surviving the toxin treatment and in vivo testing against pathogen revealed that the majority of these plants were less sensitive.
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    URL: http://dx.doi.org/10.1007/BF00247566
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    In vitro expression of genes affecting whole plant phenotype — the effect of Rht/Gai alleles on the callus culture response of wheat (Triticum aestivum L. em. Thell) (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 474-479 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Callus ; Regeneration ; Dwarfinggenes ; Rht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calli were initiated from immature embryos of 12 lines of hexaploid wheat (Triticum aestivum L. em. Thell). The lines were from 3 varieties — April Bearded, Bersee and Maris Huntsman — isogenic for the reduced height/gibberellic acid insensitivity (Rht) genes — Rht1, Rht2 and Rht3 — and the ‘tall’ (rht) allele. The dwarfing genes had significant effects on the growth and morphogenesis of calli. The genes interacted with the 2,4-D in the medium and the varietal background. Calli of each line were cultured in the presence and absence of 1 mg/l of gibberellic acid (GA), but there was no interaction of the Rht genes with GA in vitro. The effect of the Rht genes is discussed in relation to their effects on cellular hormone metabolism and their involvement in previously described chromosome 4B effects in culture.
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    URL: http://dx.doi.org/10.1007/BF00276752
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    Linkage mapping of genes controlling endosperm storage proteins in wheat (1988)
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    Theoretical and applied genetics 75 (1988), S. 642-650 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; HMW glutenin subunits ; Linkage mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A translocation mapping procedure was used to map the Glu-1 genes, controlling high-molecular-weight (HMW) glutenin subunits on the long arms of group 1 wheat chromosomes, with respect to their centromeres. The Glu-A1 genes on chromosome arm 1AL were linked with the centromere but gave very different map distances (in cM) of 10.2 ± 3.5 and 33.9 ± 5.4 with test-cross and F2 progeny, respectively. The centromere-Glu-B1 and centromere-Glu-D1 map distances were estimated to be 28.1 ± 2.8 and 30.9 ± 2.7, respectively, using test-cross seeds. A telocentric mapping procedure gave a reduced map distance of 22 ± 3.5 for the centromere-Glu-D1 interval. Possible reasons for the differences between the mapping results obtained with test-cross and F2 seeds and with the translocation and telocentric procedures are discussed.
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    URL: http://dx.doi.org/10.1007/BF00289133
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    Genetic analysis of anther culture response in wheat using aneuploid, chromosome substitution and translocation lines (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 7-11 
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    ISSN: 1432-2242
    Keywords: Wheat ; Anther culture ; Regeneration ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Marked effects of genotype on wheat anther culture response have been observed. Genetic factors have been recognised to be one of the major contributors to in vitro responses of cultured wheat tissues. In wheat anther culture, embryo induction, plant regeneration and albina/green ratio have been determined to be heritable traits. Using Chinese Spring (CS) monosomic 1D, single chromosome substitution lines of chromosome 5B or chromosome arm 5BL from Chinese Spring into six varieties, and F1 hybrids heterozygous for the 1B chromosome structure (1BL-1BS/1BL-1RS), the anther culture response was studied: genes on CS1D chromosome and 5BL chromosome arm increases the embryo frequency; gene(s) involved in regeneration ability are located on the 1RS chromosome arm; a gene increasing albina frequency is located on Chinese Spring 5B chromosome. Our results support the fact that without gametic selection, a differential development occurred from the particular classes of microspores carrying genes for higher regeneration ability. Moreover, in some crosses, a few genes with major effects were involved in determination of anther culture response.
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    URL: http://dx.doi.org/10.1007/BF00292308
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    Increasing grain protein content of hard red winter wheat (Triticum aestivum L.) by mutation breeding (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 41-46 
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    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Protein ; Mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Poor adaptability or functional quality of much germplasm used for breeding high-protein hard red winter wheats prompted mutagenesis as an alternative means of increasing grain protein content. Four hard red winter wheat genotypes — KS644 (‘Triumph// Concho/Triumph’), ‘Kaw’, ‘Parker’, and ‘Shawnee’ — were treated with 0.40 M ethyl methanesulfonate (EMS). Advanced lines (M8-M10) were selected that had a 3-year mean grain protein advantage of 0.7% to 2.0% over controls. Increased grain protein content was generally associated with decreased grain yield and kernel weight, but some high-protein mutant lines had yields or kernel weights similar to those of original genotypes. Changes in height and lodging induced by EMS were generally favorable, most mutants being shorter and lodging less than controls, but blooming date was generally delayed, a deleterious change. One line also changed from resistant to segregating for wheat soil-borne mosaic virus. Mutant lines might be utilized in cross-breeding programs, particularly if negative pleiotropic effects and linkages are absent.
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    URL: http://dx.doi.org/10.1007/BF00276260
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    Glutenin subunits of genetically related European hexaploid wheat cultivars: Their relation to bread-making quality (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 107-111 
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    ISSN: 1432-2242
    Keywords: Glutenin ; Wheat ; Bread-making ; Chromosomal control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The subunit composition of glutenin from 47 European wheat cultivars was studied using SDS-polyacrylamide gel electrophoresis. These cultivars are genetically related since they originate from the same stock. Moreover, the diversity of sample, containing cultivars with very different French bread-making qualities, makes it possible to investigate the relationship between glutenin subunit composition and bread-making quality. 16 electrophoretic types of glutenin subunits could be distinguished: these were grouped into four classes. Depending on the cultivar, six to eight glutenin subunits with MW more than or equal to 62,000 were detected. Subunits 3 and 5, with an approximate MW of 122,000 and 108,000 respectively, seem to play a prominent role on bread-making quality; they were found in cultivars of good quality and were absent in those unsuitable for making French bread. Two other subunits (9 and 10; MW: 71,000 and 66,000, respectively) have a less defined influence but may be needed in some types of glutenin structure. Aneuploid analysis shows that in ‘Chinese Spring’, subunit 5 is coded by a gene on the long arm of chromosome 1B. The location of genes coding for subunits 3, 9 and 10 could not be determined.
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    URL: http://dx.doi.org/10.1007/BF00263099
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    Genetic studies of frost resistance in wheat (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 145-152 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Frost resistance ; Diallel cross ; F2monosomics ; Substitutions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic studies of frost resistance were performed on various wheat varieties using diallel, F2 monosomic and substitution analysis. A six-parental cross including reciprocals was carried out, and F1 hybrids and their parents were used for the freezing tests under controlled conditions. Both the general combining ability (GCA) and the specific combining ability (SCA) were significant, indicating additive and non-additive gene action in the inheritance of frost resistance. The high GCA∶SCA ratio revealed a preponderance of additive genetic variance. No significant reciprocal differences were found between the reciprocal crosses. The variance/covariance graphical analysis indicated the partial dominance of frost sensitivity. Frost sensitive varieties had the largest number of dominant genes, while frost resistant varieties had the highest proportion of recessive genes. The magnitude of the additive component of variation was higher than that of the dominance component, and the overall measure of the degree of dominance was smaller than one, so average dominance is incomplete. The increasing and decreasing alleles are not equally frequent at all loci. In this set of wheat varieties the values of narrow and broad heritability are relatively high. F2 monosomic analysis of the winter wheat variety ‘Arthur’ crossed with the monosomics of ‘Chinese Spring’ revealed that the average frost resistance of all the 21 monosomics was lower than that of the disomic. F2 monosomic hybrids 5A, 2B, 4B and 5D proved to be relatively frost resistant, while monosomics 3A, 3B and 6D were the most sensitive. The control of frost resistance in the set of chromosome substitution lines of the variety ‘Cheyenne’ into ‘Chinese Spring’ (with the exception of 2B) indicated that the genes responsible for the frost resistance of ‘Cheyenne’ are localised in chromosomes 5A, 7A, 4B, 5B, 4D and 5D. The genetic basis of frost resistance and problems of analysis are discussed.
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    URL: http://dx.doi.org/10.1007/BF00264968
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    Monosomic analysis of genes for resistance against stem rust races in bread wheat (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 313-316 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Puccinia graminis ; Stem rust resistance ; Monosomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using monosomic analysis genes governing resistance in one wheat variety, ‘E5883’, against stem rust races 15C and 122, were located on specific chromosomes. Against race 15C, a major dominant gene, Sr6, was located on chromosome 2D of ‘E5883’ with minor modifiers found on chromosomes 4A and 6D. Against race 122, the presence of a recessive hemizygous ineffective gene, Sr8, was identified, located on chromosome 6A of variety ‘E5883’.
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    URL: http://dx.doi.org/10.1007/BF00264984
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    Analysing environments for plant breeding purposes as exemplified by multivariate analyses of long term wheat yields (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 317-325 
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    ISSN: 1432-2242
    Keywords: Environmental evaluation ; Plant breeding ; Multivariate analysis ; Wheat ; Climatic variable
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A previous attempt to relate long term yields from the Western Australian wheat belt to climatic factors proved only partially successful. Here, principal component analysis has been used to examine the patterns of variability created by those socio-economic factors which may have obscured any underlying relationship which existed between yield and climate. In fact, these analyses revealed the existence of variation peculiar to particular groups of years, a result which could explain why many attempts to relate crop yields directly to climatic factors have proved unsuccessful. The plant breeding implications of these genotype x environment interactions are considered.
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    URL: http://dx.doi.org/10.1007/BF00264985
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    Genetic variation in wheat endosperm proteins: an analysis by two-dimensional electrophoresis using intervarietal chromosomal substitution lines (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 361-371 
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    ISSN: 1432-2242
    Keywords: Wheat ; Endosperm ; Proteins ; Electro phoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The major endosperm proteins in a range of genotypes of hexaploid wheat have been fractionated by two-dimensional electrophoresis. The genotypes included nine varieties and forty four intervarietal substitution lines in which chromosomes 1A, 1B, 1D, 6A, 6B or 6D from eight of the varieties have been introduced one at a time into a common genetic background. The appearance of different protein subunits was often correlated with a chromosome substitution. This showed that many of the genes for the high molecular weight protein subunits (molecular weight range 55,000 to 140,000 determined by SDS polyacrylamide gel electrophoresis) are specified by chromosomes 1A, 1B and 1D while many of the lower molecular weight subunits (molecular weight range 30,000 to 45,000) are specified by chromosomes 6A, 6B and 6D. The different protein subunits correlated with chromosome substitution could not always be recognised in the varietal source of the substituted chromosome. The different subunits specified by homologous chromosomes in different wheat varieties may differ in isoelectric point and/or molecular weight.
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    URL: http://dx.doi.org/10.1007/BF00276450
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    Biochemical markers associated with two Mv chromosomes from Aegilops ventricosa in wheat-Aegilops addition lines (1981)
    Springer
    Theoretical and applied genetics 60 (1981), S. 5-10 
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    ISSN: 1432-2242
    Keywords: Wheat ; Aegilops ventricosa ; Addition lines ; Biochemical markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The distribution of three biochemical markers, U-1, CM-4 and Aphv-a, -b, among wheat-Aegilops addition lines carrying Mv chromosomes from Aegilops ventricosa (genomes DvMv) has been investigated. Addition lines which had been previously grouped together on the basis of common non-biochemical characters carried marker U-1, a protein component from the 2M urea extract. The added chromosome, in the appropriate genetic background, seems to confer a high level of resistance to the eyespot disease, caused by the fungus Cercosporella herpotrichoides. The other two markers were concomitantly associated with another similarly formed group of addition lines. Both CM-4, a protein component from the chloroform:methanol extract, and Aphv-a, -b, alkaline phosphate isozymes, have been previously shown to be associated with homoeologous chromosome group 4, which suggests that the added chromosome in the second group of addition lines is 4Mv.
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    URL: http://dx.doi.org/10.1007/BF00275171
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    The role of ‘Norin 10’ dwarfing genes in photosynthetic and respiratory activity of wheat leaves (1981)
    Springer
    Theoretical and applied genetics 60 (1981), S. 81-84 
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    ISSN: 1432-2242
    Keywords: Wheat ; ‘Norin 10’ dwarfing genes ; Photosynthesis ; Respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparative analysis of eight cultivars of spring wheat (Triticum aestivum) classified by height as tall (T), semi-dwarf (D1), dwarf (D2) and very dwarf (D3) was conducted to study their efficiency of oxygen exchange during photosynthesis and dark respiration. Two cultivars were included in each height group. Cultivars carrying ‘Norin 10’ dwarfing genes (D1, D2 and D3) were found to have a significantly higher photosynthetic rate per unit leaf area than talls (T) that lack these genes. Among the ‘Norin’ gene carriers, dwarf group (D2) was most efficient, followed by very dwarf (D3) and semi-dwarf (D1). Photosynthetic rate and respiratory rate were found to have a positive relationship.
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    URL: http://dx.doi.org/10.1007/BF00282421
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    Investigation of a preferentially transmitted Aegilops sharonensis chromosome in wheat (1982)
    Springer
    Theoretical and applied genetics 61 (1982), S. 27-33 
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    ISSN: 1432-2242
    Keywords: Preferential transmission ; In situ hybridization ; C-banding ; Aegilops sharonensis ; Wheat ; Addition ; Substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An attempt to produce a set of addition lines of Aegilops sharonensis to the wheat variety ‘Chinese Spring’ produced only one addition line. This was due to preferential transmission of one chromosome from Ae. sharonensis. This chromosome was studied in detail by established cytological methods of chromosome observation and by the newer techniques of C-banding and in situ hybridization of a cloned DNA sequence. The chromosome was found to be partially homologous to an Ae. sharonensis chromosome of similar behaviour in another wheat addition line. The incomplete homology of the two Ae. sharonensis chromosomes was due to the presence of a translocated segment of a wheat chromosome. — Substitution lines of the Ae. sharonensis chromosome for wheat homoeologous group 4 were produced and the Ae. sharonensis chromosome thereby designated 4 S l .
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    URL: http://dx.doi.org/10.1007/BF00261506
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    Preferential C-banding of wheat or rye chromosomes (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 227-233 
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    ISSN: 1432-2242
    Keywords: C-banding ; Wheat ; Rye ; Triticale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using different stains, wheat chromosomes could be distinguished from rye chromosomes by preferential staining. C-bands of rye chromosomes were preferentially stained with Giemsa while those of wheat chromosomes were preferentially stained with either Leishman or Wright stain. Preferential staining aids the identification of wheat and rye chromosomes and chromosome segments and in particular the recognition of wheat/rye chromosome substitutions and translocations.
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    URL: http://dx.doi.org/10.1007/BF00304000
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    Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones (1983)
    Springer
    Theoretical and applied genetics 67 (1983), S. 87-96 
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    ISSN: 1432-2242
    Keywords: HMW glutenin subunit genes ; cDNA clones ; Tandem DNA repeats ; Chromosomal location ; Gene copy number ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary cDNA clones encoding wheat HMW glutenin subunits have been isolated from a cDNA bank made to poly A+ RNA from developing wheat endosperm var. Chinese Spring. One such clone, pTag 1290, has enabled us to identify the HMW glutenin mRNA species. The DNA sequence of this clone has been partially determined and it contains several tandem DNA repeats. The sequence is discussed in relation to the generation of the HMW glutenin subunit gene family. Analysis of the organization of the HMW glutenin sequences in the wheat genome revealed that the genes encoding HMW glutenin subunits exist in low copy number and are located on the long arm of each of the homoeologous group 1 chromosomes.
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    URL: http://dx.doi.org/10.1007/BF00303930
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    Responses of alloplasmic (cytoplasm=Triticum timopheevii) and euplasmic wheats (Triticum aestivum) to photoperiod and vernalization (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 61-66 
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    ISSN: 1432-2242
    Keywords: Triticum aestivum ; T. timopheevii ; Wheat ; Photoperiod ; Vernalization ; Male sterility ; Alloplasmic hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Studies were conducted to determine the influence of the male sterility-inducing cytoplasm of Triticum timopheevii (Zhuk.) Zhuk. on response of several common winter wheat (T. aestivum L.) nuclear genotypes to photoperiod and vernalization. Comparative studies of cytoplasmic substitution lines provide information on the role of the cytoplasmic genetic mechanism in growth and development. In the case of cytoplasmic male sterility-based hybrid production systems, ubiquity of sterility-inducing cytoplasm in derived hybrids warrants thorough characterization of its influence on plant phenotype. Factorial combinations of cytoplasm (T. timopheevii and T. aestivum), nuclear genotype, and photoperiod or vernalization treatments were evaluated under hydroponic conditions in controlled environment chambers. Interaction of cytoplasm, photoperiod, and nuclear genotype was significant in one or more experiments for days to anthesis and potential spikelet number, and interaction of cytoplasm, vernalization, and nuclear genotype was significant for days to spike emergence. Long day length was associated with increased percentage seed set in one study, but interactions of photoperiod and cytoplasm were not detected for percentage seed set. Interactions involving cytoplasm and photoperiod or vernalization were interpreted as evidence of the existence of genetic factors in cytoplsam of T. timopheevii which alter photoperiod or vernalization responses of alloplasmic plants relative to responses exhibited by euplasmic plants. Since photoperiod and vernalization responses are critical to adaptation, T. timopheevii cytoplasm can alter adaptability of T. aestivum. The specific effect would be nuclear genotype dependent, and does not appear to be of a magnitude greater than that induced by nuclear genetic variability at loci conditioning photoperiod or vernalization responses or other adaptation-determining characteristics. Normal multilocation/year testing of alloplasmic hybrids should therefore adequately identify zones of adaptation.
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    URL: http://dx.doi.org/10.1007/BF00281850
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    Identification and chromosomal locations of aconitase gene loci in Triticeae species (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 261-268 
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    ISSN: 1432-2242
    Keywords: Aconitase ; Isozymes ; Structural genes ; Triticeae ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two systems of monomeric aconitase (ACO) isozymes, designated ACO-1 and ACO-2, were identified in Triticum aestivum and in five diploid Triticeae species. The gene loci Aco-A1, Aco-B1, and Aco-D1 were located in T. aestivum cv. ‘Chinese Spring’ chromosome arms 6Aq, 6Bq, and 6Dq, respectively, and the gene loci Aco-A2, Aco-B2, and Aco-D2 in 5 Aq, 5 Bq, and 5Dq, respectively. Aco-1 gene loci were also identified in 6Eβ of Elytrigia elongata, 6HL of Hordeum vulgare cv. ‘Betzes’, 6RL of Secale cereale ‘PI 252003’, 6S1 of T. longissimum, and CSU-31 of T. umbellulatum. Other Aco-2 gene loci were identified in 5RL of S. cereale cv. ‘King II’ and 4EL of E. elongata. Conservation of synteny relationships is indicated among the species studied for the genes identified, with the exception of Aco-E2; the presence of this gene in 4EL suggests that E. elongata differs from ‘Chinese Spring’ and ‘King II’ by a translocation involving 4E and 5E.
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    URL: http://dx.doi.org/10.1007/BF00289978
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    The effect of the 4B chromosomes of hexaploid wheat on the growth and regeneration of callus cultures (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 439-444 
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    ISSN: 1432-2242
    Keywords: Wheat ; Callus ; Regeneration ; Tissue culture ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calli were initiated from immature embryos of nine lines of hexaploid wheat (Triticum aestivum L. em. Thell). These were the euploid lines Chinese Spring and Cappelle-Desprez, a line of Chinese Spring ditelocentric for the long arm of 4B, four substitution lines of Chinese Spring in which chromosome 4B has been replaced by its homologues from different wheat varieties and substituted into Chinese Spring and a substitution line of Besostaya I 4B into Cappelle-Desprez. The calli from these lines were found to differ in their growth rates and morphogenic and regenerative activities. The substitution of different 4B chromosomes into Chinese Spring significantly increased morphogenesis and shoot regeneration from callus. The potential for developing wheat lines with improved culture characteristics is discussed.
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    URL: http://dx.doi.org/10.1007/BF00289818
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    Hybrid seed development and viability in crosses of T. aestivum (cv. Chinese Spring) monosomic lines with S. cereale (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 503-507 
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    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Crossing barrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The diploid and tetraploid wheats have a crossing barrier that inhibits development and viability of the F1 hybrid seeds which result from their pollination with rye. This barrier is ineffective in synthetic or natural hexaploid wheat × rye crosses. From the results obtained by crossing the Chinese Spring monosomic series to a diploid rye composite, it was concluded that the breakdown of this barrier in hexaploid wheats is determined by polygenes, but may also involve genedosage effects. While more than half of the hexaploidwheat chromosomes may contribute to the breakdown of the barrier, chromosome 1D had the strongest effect. Its absence resulted in shrivelled and inviable hybrid kernels similar to those obtained when the T. durum cultivar Langdon was crossed with rye.
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    URL: http://dx.doi.org/10.1007/BF00289829
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    Effect of population structure on protein-yield improvements in spring wheat (Triticum aestivum L. em Thell) (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 625-632 
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    ISSN: 1432-2242
    Keywords: Wheat ; Grain yield ; Grain protein ; Population structure ; Intermating ; Partial backcrossing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a study designed to develop a more efficient breeding method for concurrent protein-yield improvements in wheat (Triticum aestivum L. em. Thell), 7 base populations [2 F2's, 1 intermated F2 (IF2) and 4 partial backcross (PBC) populations] developed from biparental crosses involving 2 Canadian hard red spring (CHRS) and 2 Canadian utility (CU) wheat cultivars were evaluated in Winnipeg, Manitoba, Canada. The IF2 and PBC populations were generated for comparison with conventional F2 populations and to determine which of the 4 methods of population development would provide a more efficient means of producing potentially superior genetic recombinants. Parameters pertaining to means, variances, correlations, heritabilities and frequencies of desirable and undesirable progenies were used to evaluate the limitations to genetic gain that may be expected from selection for GY and GPC in F2, IF2, CHRS-PBC and CU-PBC populations. Analysis of protein and yield data from 105 S1 lines derived from each of the 7 populations showed the CU-PBC's to have the highest grain yield (GY) and the lowest grain protein concentration (GPC) means; and the CHRS-PBC's, the lowest GY and the highest GPC means. The F2 and IF2 populations were intermediate for both characteristics. Populations developed from the same biparental cross did not differ significantly with respect to the majority of genetic parameters. However, desirable progenies combining high GY with high GPC were more frequent in the CU-PBC, and least frequent in the CHRS-PBC populations. The observed superiority of the CU-PBC populations appeared to be related to the advantage the system has in preserving the genetic integrity of a proven cultivar, while adding desirable genetic factors from another cultivar, thus capitalizing on introgression and upgrading simultaneously.
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    URL: http://dx.doi.org/10.1007/BF00288862
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    The inheritance of seed peroxidases of wheat and rye: further data (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 767-772 
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    ISSN: 1432-2242
    Keywords: Triticum ; Secale ; Wheat ; Rye ; Peroxidases ; Inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Further data on the inheritance of seed peroxidases of hexaploid wheat (Triticum aestivum L.) and rye (Secale cereale L.) have been obtained from the genetic analysis of several progenies of both species. Additional data on the inheritance and the chromosomal location and linkage have been obtained for peroxidases of wheat embryo and rye endosperm. The general presence of null alleles in peroxidase loci has been confirmed in both species. In addition to simple monogenic inheritance, epistatic segregations have been observed in both species. These epistatic segregations again suggest the presence of “regulatory” genes controlling the expression of individual peroxidases in both species and also the existence of several duplicate homoeologous genes in wheat. Known linkage relationships have been confirmed and new ones are indicated. Loci for embryo wheat peroxidases seem to be in chromosomes of the homoeology group 3. The rye endosperm ones should be in chromosome 7R, although it is hypothesized that a duplication of gene EPer1 is located in chromosomes 4R and 7R.
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    URL: http://dx.doi.org/10.1007/BF00247555
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    The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 117-126 
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    ISSN: 1432-2242
    Keywords: Glutenin ; Wheat ; Amino acid sequences ; Chromosomes 1A, 1B and 1D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide and deduced amino acid sequences of a high molecular weight glutenin subunit gene derived from chromosome 1B of bread wheat (Triticum aestivum L.) are reported. The encoded protein corresponds to the y-type subunit 1B9. Comparison of the 5′ upstream untranslated regions of this gene and a previously reported silent y-type gene derived from chromosome 1A showed a deletion of 85 bp in the latter. A sequence present in this region of the 1By 9 gene shows homology with part of the “-300 element” which is conserved in the 5′ upstream regions of other prolamin genes from barley, wheat and maize (Forde BG et al. 1985). It is suggested that the absence of this element is responsible for the lack of expression of the 1Ay gene. Comparison of the derived amino acid sequence with those reported previously for the silent 1Ay gene and the expressed x-type (1Dx2) and y-type (1Dy12) genes derived from chromosome 1D showed that the three y-type proteins are closely related. In contrast the x-type subunit (1Dx2) shows clear differences in the N-terminal region and in the number, type and organisation of repeats in the central repetitive domain.
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    URL: http://dx.doi.org/10.1007/BF00249151
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    The ribosomal RNA genes in synthetic tetraploids of wheat (1987)
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    Theoretical and applied genetics 75 (1987), S. 138-143 
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    ISSN: 1432-2242
    Keywords: Wheat ; Ribosomal RNA genes ; Synthetic tetraploids ; Evolution ; Nucleolar activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ribosomal RNA genes in the A genome of tetraploid (AABB) wheats are either absent or present at low levels. In four synthetic AABB tetraploids tested by in situ hybridization with a radioactive probe, the level of radioactive label at A and B NOR's corresponded to those of the parent species. There was no reduction after ten generations. In addition, the A chromosome rDNA is transcriptively active, the numbers of nucleoli being governed by the number of rRNA gene repeats in the smaller of the two A-genome NOR's. Since the wild tetraploid Triticum dicoccoides, as well as its domesticated derivatives, lack A-genome NOR's, their deletion is likely to have occurred prior to domestication, i.e. more than 10,000 years ago.
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    URL: http://dx.doi.org/10.1007/BF00249154
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    Location of β-amylase sequences in wheat and its relatives (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 286-290 
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    ISSN: 1432-2242
    Keywords: RFLP ; Wheat ; Aegilops ; Rye ; β-Amylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A β-amylase cDNA clone isolated from barley has been used to locate β-amylase encoding sequences on wheat, rye, and Aegilops umbellulata chromosomes by hybridisation to restriction endonuclease digested DNA obtained from wheat aneuploid and wheat-alien addition lines. Structural genes were identified on homoeologous group 4 and 5 chromosomes, confirming the results of isozyme studies. In addition, a further set of structural genes was found on homoeologous group 2 chromosomes. It is proposed that there are two homoeoallelic series, β-Amy-1 on group 4 or 5 chromosomes, and β-Amy-2 on group 2 chromosomes. Evidence is presented that each locus contains one or two β-amylase structural genes, and it is suggested that the large number of isozymes seen upon IEF are due to post-translational modifications.
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    URL: http://dx.doi.org/10.1007/BF00303966
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    Cytogenetic investigations in rye, wheat and triticale (1988)
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    Theoretical and applied genetics 75 (1988), S. 305-308 
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    ISSN: 1432-2242
    Keywords: Wheat ; Giemsa/Leishman ; C-banding ; Polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chromosomes of tetra- and hexaploid wheat have been individually characterized by Giemsa and/or Leishman C-banding techniques. Appropriate methodological modifications resulted in almost identical staining of chromosomes of tetraploid wheat with Giemsa and Leishman solutions. Additionally comparison of Giemsa banded chromosomes of the A- and B-genome of Triticum turgidum 34 and Triticum aestivum cv ‘Jubilar’ reveals similar or corresponding patterns in all homologous chromosomes with the exception of chromosome 7B. Apart from this intervarietal variation in certain homologous chromosomes of both wheat cultivars, intravarietal polymorphism is verified.
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    URL: http://dx.doi.org/10.1007/BF00303969
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    Ribosomal DNA as a convenient probe to follow segregation and possible divergency from expected homozygosity after haploidization of an androgenetic process (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 389-396 
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    ISSN: 1432-2242
    Keywords: Ribosomal DNA ; Restriction fragment length polymorphism ; Anther-derived doubled haploids ; Gametoclonal variation ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism of the wheat nuclear ribosomal DNA has been studied in several steps of a breeding scheme, including parental genotypes, F1 hybrid, F9 generation, and anther-derived doubled haploid lines obtained from F9. Ribosomal DNA represents a suitable molecular marker in following segregation and possible divergency from expected homozygosity after haploidization of an androgenetic process. It has been shown to undergo variations among the first cycle-doubled haploid lines in the relative amount of two different sizes of ribosomal DNA repeat units. The specificity and peculiar properties of the plant system used allowed us to assign an intrachromosomal location (short arm of the chromosomes 1B, 1R or 6B) to several ribosomal DNA repeat units that differ by the length of their nontranscribed spacer region.
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    URL: http://dx.doi.org/10.1007/BF00276740
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    Linkage mapping of genes controlling endosperm storage proteins in wheat (1988)
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    Theoretical and applied genetics 75 (1988), S. 628-641 
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    ISSN: 1432-2242
    Keywords: Wheat ; Triplet proteins ; Gliadins ; Glutenins ; Linkage mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A translocation mapping procedure was used to map gene-centromere distances for the genes controlling endosperm proteins on the short arm of each of the chromosomes 1A, 1B and 1D in wheat. The genes controlling triplet proteins (tentatively designated Tri-1) were found to be closely linked to the centromere on chromosome arms 1AS and 1DS and loosely linked to the gliadin genes (Gli-1) on the same arms. The Gli-1 genes segregated independently or were very loosely linked to their respective centromeres. The Gli-B1-centromere map distance on 1BS was also estimated using conventional telocentric mapping and the result was similar to that obtained with the translocation mapping. A simple two-step one-dimensional electrophoretic procedure is described which allows the low-molecular-weight (LMW) glutenin subunits to be separated from the gliadin bands, thus facilitating the genetic analysis of these LMW subunits. No recombination was observed between the genes (designated Glu-3) controlling some major LMW glutenin subunits and those controlling gliadins on chromosome arms 1AS and 1DS. However, in a separate experiment, the genes controlling LMW glutenin subunits on 1BS (Glu-B3) showed a low frequency of recombination with the gliadin genes.
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    URL: http://dx.doi.org/10.1007/BF00289132
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    The production of haploid wheat plants from wheat x maize crosses (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 393-397 
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    ISSN: 1432-2242
    Keywords: Wheat ; Maize ; Wide-crosses ; Embryo culture ; Haploids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hybrid embryos from hexaploid wheat x maize crosses rapidly lose the maize chromosomes to produce haploid wheat embryos. Such embryos almost always aborted when left to develop on the plant, and only 1 was recovered from 2440 florets (0.17% of the expected number). Embryos had greater viability in spikelet culture, 47 (26.5% of the expected number) being recovered from 706 ovaries. Thirty-two of these embryos germinated to give green plants, 31 of which were haploid (21 wheat chromosomes) and 1 of which was euploid (42 wheat chromosomes). Spikelet culture enabled 17.1% of the expected number of embryos to be recovered as haploid plants, a 100-fold improvement on allowing embryos to develop in vivo. Ten haploid plants of ‘Chinese Spring’ (kr1, kr2), 13 plants of ‘Chinese Spring (Hope 5A)’ (kr1, Kr2), and 8 of ‘Hope’ (Kr1, Kr2) were recovered. The potential of wheat x maize crosses for wheat haploid production and for gene transfer from maize to wheat is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265339
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