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  • photosynthesis  (224)
  • 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
  • Mt. Etna
  • Springer  (244)
  • 2020-2023
  • 2005-2009  (19)
  • 1995-1999  (225)
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  • 1
    Publication Date: 2020-11-16
    Description: Geological, geophysical and geotechnical investigations, for the characterization of the strong-motion recording sitesmanaged by the ItalianCivil Protection, have been carried out in the framework of the project “Italian strong-motion database in the period 1972–2004”. The project aimed at creating an updated database of strong-motion data acquired in Italy by different institutions in the time span 1972–2004, and at improving the quality of disseminated data. This article illustrates the state of the recording site characterization before the beginning of the project, explains the criteria adopted to select the sites where geophysical/ geotechnical investigation have been performed and describes the results of the promoted field surveys.
    Description: Published
    Description: 1189–1207
    Description: 4IT. Banche dati
    Description: JCR Journal
    Description: open
    Keywords: site ; characterization ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 2
    Publication Date: 2021-06-21
    Description: Earthquake early warning systems (EEWS), based on real-time prediction of ground motion or structural response measures, may play a role in re- ducing vulnerability and/or exposure of buildings and lifelines. Indeed, seismologists have recently developed efficient methods for real-time es- timation of an event’s magnitude and location based on limited informa- tion of the P-waves. Therefore, when an event occurs, estimates of magni- tude and source-to-site distance are available, and the prediction of the structural demand at the site may be performed by Probabilistic Seismic Hazard Analysis (PSHA) and then by Probabilistic Seismic Demand Analysis (PSDA) depending upon EEWS measures. Such an approach contains a higher level of information with respect to traditional seismic risk analysis and may be used for real-time risk management. However, this kind of prediction is performed in very uncertain conditions which may affect the effectiveness of the system and therefore have to be taken into due account. In the present study the performance of the EWWS under development in the Campania region (southern Italy) is assessed by simu- lation. The earthquake localization is formulated in a Voronoi cells ap- proach, while a Bayesian method is used for magnitude estimation. Simu- lation has an empirical basis but requires no recorded signals. Our results, in terms of hazard analysis and false/missed alarm probabilities, lead us to conclude that the PSHA depending upon the EEWS significantly improves seismic risk prediction at the site and is close to what could be produced if magnitude and distance were deterministically known.
    Description: Published
    Description: 211-232
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: reserved
    Keywords: Earthquake Early ; Campania Region ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: book chapter
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  • 3
    Publication Date: 2017-04-04
    Description: In order to empirically obtain the scaling relationships for the high-frequency ground motion in the Western Alps (NW Italy), regressions are carried out on more than 7500 seismograms from 957 regional earthquakes. The waveforms were selected from the database of 6 three-component stations of the RSNI (Regional Seismic network of Northwestern Italy). The events,MW ranging between 1.2 and 4.8, were recorded within a hypocentral distance of 200 km during the time period: 1996–2001. The peak ground velocities are measured in selected narrow-frequency bands, between 0.5 and 14 Hz. Results are presented in terms of a regional attenuation function for the vertical ground motion, a set of vertical excitation terms at the reference station STV2 (hard-rock), and a set of site terms (vertical and horizontal), all relative to the vertical component of station STV2. The regional propagation of the ground motion is modeled after quantifying the expected duration of the seismic motion as a function of frequency and hypocentral distance. A simple functional form is used to take into account both the geometrical and the anelastic attenuation: a multi-variable grid search yielded a quality factor Q( f ) = 310 f 0.20, together with a quadri-linear geometrical spreading at low frequency. A simpler, bilinear geometrical spreading seems to be more appropriate at higher frequencies (f 〉 1.0 Hz). Excitation terms are matched by using a Brune spectral model with variable, magnitude-dependent stress drop: at Mw 4.8, we used σ = 50MPa. A regional distanceindependent attenuation parameter is obtained (κ0 = 0.012 s) by modelling the average spectral decay at high frequency of small earthquakes. In order to predict the absolute levels of ground shaking in the region, the excitation/attenuation model is used through the Random Vibration Theory (RVT) with a stochastic point-source model. The expected peak-ground accelerations (PGA) are compared with the ones derived by Ambraseys et al. (1996) for the Mediterranean region and by Sabetta and Pugliese (1996) for the Italian territory.
    Description: Published
    Description: 315-333
    Description: JCR Journal
    Description: reserved
    Keywords: Attenuation ; Ground motion ; Western Alps ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 4
    Publication Date: 2017-04-04
    Description: For early-warning applications in particular, the reliability and efficiency of rapid scenario generation strongly depend on the availability of reliable strong ground-motion prediction tools. If shake maps are used to represent patterns of potential damage as a consequence of large earthquakes, attenuation relations are used as a tool for predicting peak ground-motion parameters and intensities. One of the limitations in the use of attenuation relations is that these have only rarely been retrieved from data collected in the same tectonic environment in which the prediction has to be performed. As a consequence, strong ground motion can result in underestimations or overestimations with respect to the recorded data. This also holds for Italy, and in particular for the Southern Apennines, due to limitations in the available databases, both in terms of distances and magnitude. Moreover, for “real-time” early-warning applications, it is important to have attenuation models for which the parameters can be easily upgraded when new data are collected, whether this has to be done during the earthquake rupture occurrence or in the post-event, when all the strong motion waveforms are available. Here we present a strong-motion attenuation relation for early-warning applications in the Campania region (Southern Apennines), Italy. The model has a classical analytical formulation, and its coefficients were retrieved from a synthetic strong-motion database created by using a stochastic approach. The input parameters for the simulation technique were obtained through the spectral analysis of waveforms of earthquakes recorded by the Istituto Nazionale di Geofisica e Vulcanologia (INGV) network for a magnitude range Md (1.5,5.0) in the last fifteen years, and they have been extrapolated to cover a larger range. To validate the inferred relation, comparisons with two existing attenuation relations are presented. The results show that the calibration of the attenuation parameters, i.e., geometric spreading, quality factor Q, static stress drop values along with their uncertainties, are the main concern.
    Description: Published
    Description: 133-152
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: reserved
    Keywords: A Strong Motion ; Earlywarning ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: book chapter
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  • 5
    Publication Date: 2017-04-04
    Description: In the framework of an ongoing project financed by the Campania Region, a prototype system for seismic early and post-event warning is being developed and tested, based on a dense, wide dynamic seismic network (ISNet) and under installation in the Apennine belt region. This paper reports the characteristics of the seismic network, focussing on the required technological innovation of the different seismic network components (data-logger, sensors and data communication). To ensure a highly dynamic recording range, each station is equipped with two types of sensors: a strong-motion accelerometer and a velocimeter. Data acquisition at the seismic stations is performed using Osiris-6 model data-loggers made by Agecodagis. Each station is supplied with two (120 W) solar panels and two 130 Ah gel cell batteries, ensuring 72-h autonomy for the seismic and radio communication equipment. The site is also equipped with a GSM/GPRS programmable control/alarm system connected to several environmental sensors (door forcing, solar panel controller, battery, fire, etc) and through which the site status is known in real time. The data are stored locally on the hard-disk and, at the same time, continuously transmitted by the SeedLink protocol to local acquisition/analysis nodes (Local Control Center) via Wireless LAN bridge. At each LCC site runs a linux Earthworm system which stores and manages the acquired data stream. The real-time analysis system will perform event detection and localization based on triggers coming from data-loggers and parametric information coming from the other LCCs. Once an event is detected, the system will performs automatic magnitude and focal mechanism estimations. In the immediate post-event period, the RISSC performs shaking map calculations using parameters from the LCCs and/or data from the event database. The recorded earthquake data are stored into an event database, to be available for distribution and visualization for further off-line analyses. The seismic network will be completed in two stages: • Deployment of 30 seismic stations along the southern Apennine chain (to date almost completed) • Setting up a carrier-class radio communication system for fast and reliable data transmission, and installation of 10 additional seismic stations.
    Description: Published
    Description: 325 - 341
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: reserved
    Keywords: Monitoring Infrastructure ; Early-warning Applications ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: book chapter
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  • 6
    Publication Date: 2017-04-04
    Description: The area of Serravalle, sited in the northern part of the town of Vittorio Veneto (TV), NE Italy, has been the target of a seismic microzonation campaign. 10 seismic stations have been deployed for a 7 months period to record in continuous mode. Three stations were installed on bedrock outcrops and seven on sedimentary sites with variable cover thickness. Spectral analyses have been performed on the collected data-set using the Generalized Inversion Technique (GIT, e.g. Andrews, 1986). In particular, spectral ratios have been calculated for each station relatively to the average of the three reference, bedrock sites. The spectral ratios provide quantitative estimates of the seismic motion amplifications which occur in each of the monitored sites. Two sites show high values of amplification, 5 times larger than signal amplitude at the reference sites, in correspondence of well discernible peak frequencies of 5 Hz. Results for the other stations show smaller amounts of site amplification spreading over a broad range of frequencies. Sites where the highest amplifications were recorded all lie on the left bank of the Meschio River and in areas farther away from its outlet into the plain correlating with the presence of thick layers of Quaternary deposits.
    Description: Published
    Description: 31-49
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: JCR Journal
    Description: reserved
    Keywords: spectral ratios ; reference sites ; site effects ; earthquake grounf motion ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 7
    Publication Date: 2017-04-04
    Description: The Italian strong-motion database was created during a joint project between Istituto Nazionale di Geofisica e Vulcanologia (INGV, Italian Institute for Geophysics and Vulcanology) and Dipartimento della Protezione Civile (DPC, Italian Civil Protection). The aim of the project was the collection, homogenization and distribution of strong motion data acquired in Italy in the period 1972-2004 by different institutions, namely Ente Nazionale per l’Energia Elettrica (ENEL, Italian electricity company), Ente per le Nuove tecnologie, l’Energia e l’Ambiente (ENEA, Italian energy and environment organization) and DPC. Recently the strong-motion data relative to the 23th December 2009, Parma (Mw=5.4 and Mw=4.9) and to the 2009 L’Aquila sequence (13 earthquakes with 4.1Mw6.3) were included in the ITACA database (beta release). The database contains 7038 waveforms from analog and digital instruments, generated by 1019 earthquakes with magnitude up to 6.9 and can be accessed on-line at the web site http://itaca.mi.ingv.it. The strong motion data are provided in the unprocessed and processed versions. This article describes the steps followed to process the acceleration time series recorded by analogue and digital instruments. The procedures implemented involve: baseline removal, instrumental correction, band pass filtering with acausal filters, integration of the corrected acceleration in order to obtain velocity and displacement waveforms, computation of acceleration response spectra and strong motion parameters. This procedure is applied to each accelerogram, is realised to preserve the low frequency content of the records.
    Description: In press
    Description: 5.2. TTC - Banche dati di sismologia strumentale
    Description: JCR Journal
    Description: open
    Keywords: strong-motion ; processing ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 8
    Publication Date: 2017-04-04
    Description: Microgravity observations at Mt. Etna have been routinely performed as both discrete (since 1986) and continuous (since 1998) measurements. In addition to describing the methodology for acquiring and reducing gravity data from Mt. Etna, this paper provides a collection of case studies aimed at demonstrating the potential of microgravity to investigate the plumbing system of an active volcano and detect forerunners to paroxysmal volcanic events. For discrete gravity measurements, results from 1994– 1996 and 2001 are reported. During the first period, the observed gravity changes are interpreted within the framework of the Strombolian activity which occurred from the summit craters. Gravity changes observed during the first nine months of 2001 are directly related to subsurface mass redistributions which preceded, accompanied and followed the July-August 2001 flank eruption of Mt. Etna. Two continuous gravity records are discussed: a 16-month (October 1998 to February 2000) sequence and a 48-hour (26–28 October, 2002) sequence, both from a station within a few kilometers of the volcano’s summit. The 16-month record may be the longest continuous gravity sequence ever acquired at a station very close to the summit zone of an active volcano. By cross analyzing it with contemporaneous discrete observations along a summit profile of stations, both the geometry of a buried source and its time evolution can be investigated. The shorter continuous sequence encompasses the onset of an eruption from a location only 1.5 km from the gravity station. This gravity record is useful for establishing constraints on the characteristics of the intrusive mechanism leading to the eruption. In particular, the observed gravity anomaly indicates that the magma intrusion occurred ‘‘passively’’ within a fracture system opened by external forces.
    Description: Published
    Description: 769-790
    Description: 2.6. TTC - Laboratorio di gravimetria, magnetismo ed elettromagnetismo in aree attive
    Description: JCR Journal
    Description: reserved
    Keywords: Mt. Etna ; microgravity ; 04. Solid Earth::04.03. Geodesy::04.03.05. Gravity variations
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 9
    Publication Date: 2017-04-04
    Description: Mount Etna is an open conduit volcano, characterised by persistent activity, consisting of degassing and explosive phenomena at summit craters, frequent flank eruptions, and more rarely, eccentric eruptions. All eruption typologies can give rise to lava flows, which represent the greatest hazard by the volcano to the inhabited areas. Historical documents and scientific papers related to the 20th century effusive activity have been examined in detail, and volcanological parameters have been compiled in a database. The cumulative curve of emitted lava volume highlights the presence of two main eruptive periods: (a) the 1900–1971 interval, characterised by a moderate slope of the curve, amounting to 436 · 106 m3 of lava with average effusion rate of 0.2 m3/s and (b) the 1971–1999 period, in which a significant increase in eruption frequency is associated with a large issued lava volume (767 · 106 m3) and a higher effusion rate (0.8 m3/s). The collected data have been plotted to highlight different eruptive behaviour as a function of eruptive periods and summit vs. flank eruptions. The latter have been further subdivided into two categories: eruptions characterised by high effusion rates and short duration, and eruptions dominated by low effusion rate, long duration and larger volume of erupted lava. Circular zones around the summit area have been drawn for summit eruptions based on the maximum lava flow length; flank eruptions have been considered by taking into account the eruptive fracture elevation and combining them with lava flow lengths of 4 and 6 km. This work highlights that the greatest lava flow hazard at Etna is on the south and east sectors of the volcano. This should be properly considered in future land-use planning by local authorities.
    Description: Published
    Description: 407–443
    Description: 4.3. TTC - Scenari di pericolosità vulcanica
    Description: JCR Journal
    Description: reserved
    Keywords: Mt. Etna ; effusive activity ; database ; lava flow length ; eruptive fractures ; vent elevation ; hazard zonation ; 05. General::05.02. Data dissemination::05.02.03. Volcanic eruptions
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 10
    Publication Date: 2017-04-04
    Description: Flank instability and collapse are observed at many volcanoes. Among these, Mt. Etna is characterized by the spreading of its eastern and southern flanks. The eastern spreading area is bordered to the north by the EW-trending Pernicana Fault System (PFS). During the 20022003 Etna eruption, ground fracturing along the PFS migrated eastward from the NE Rift, to as far as the 18 km distant coastline. The deformation consisted of dextral en-echelon segments, with sinistral and normal kinematics. Both of these components of displacement were one order of magnitude larger (~1 m) in the western, previously known, portion of the PFS with respect to the newly surveyed (~9 km long) eastern section (~0.1 m). This eastern section is located along a pre-existing, but previously unknown, fault, where displaced man-made structures give overall slip rates (11.9 cm/year), only slightly lower than those calculated for the western portion (1.42.3 cm/year). After an initial rapid motion during the first days of the 20022003 eruption, movement of the western portion of the PFS decreased dramatically, while parts of the eastern portion continued to move. These data suggest a model of spreading of the eastern flank of Etna along the PFS, characterized by eruptions along the NE Rift, instantaneous, short-lived, meter-scale displacements along the western PFS and more long-lived centimeter-scale displacements along the eastern PFS. The surface deformation then migrated southwards, reactivating, one after the other, the NNWSSE-trending Timpe and Trecastagni faults, with displacements of ~0.1 and ~0.04 m, respectively. These structures, along with the PFS, mark the boundaries of two adjacent blocks, moving at different times and rates. The new extent of the PFS and previous activity over its full length indicate that the sliding eastern flank extends well below the Ionian Sea. The clustering of seismic activity above 4 km b.s.l. during the eruption suggests a deep décollement for the moving mass. The collected data thus suggests a significant movement (volume 〉1,100 km3) of the eastern flank of Etna, both on-shore and off-shore.
    Description: Published
    Description: 417-430
    Description: partially_open
    Keywords: Volcano spreading ; Fracturing ; Mt. Etna ; Pernicana Fault System ; NE Rift ; 04. Solid Earth::04.04. Geology::04.04.09. Structural geology ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring ; 05. General::05.02. Data dissemination::05.02.03. Volcanic eruptions ; 04. Solid Earth::04.08. Volcanology::04.08.08. Volcanic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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    Format: 998206 bytes
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  • 11
    Publication Date: 2017-04-04
    Description: A prototype system for earthquake early warning and rapid shake map evaluation is being developed and tested in southern Italy based on a dense, dynamic seismic network (accelerometers + seismometers) under installation in the Apenninic belt region (Irpinia Seismic Network). It can be classified as a regional Earthquake Early Warning System consisting of a broad-based seismic sensor network covering a portion or the entire area which is threatened by the quake's strike. The real time magnitude estimate will take advantage from the high spatial density of the network in the source region and the broad dynamic range of installed instruments. Based on the offline analysis of high quality strong-motion data bases recorded in Italy, several methods are envisaged, using different observed quantities (peak amplitude, dominant frequency, square velocity integral, …) to be measured on seismograms, as a function of time, both on P and early-S wave signals. Results from the analysis of the Italian strong motion database point out the possibility of using low-pass filtered displacement and velocity peak amplitudes measured in time windows lasting less than 3-4 sec after the first P- or S-wave arrivals. These parameters show they are robustly correlated with moment magnitude. The correlation found of 3Hz low-pass filtered PGV and PGD with magnitude is discussed and interpreted in terms of plausible dynamic models of the earthquake rupture process during its initial stage.
    Description: Published
    Description: 45-63
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: reserved
    Keywords: Real-time Estimation ; Magnitude ; Seismic Early Warning ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: book chapter
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  • 12
    Publication Date: 2017-04-04
    Description: Various authors, analysing the set of accelerograms recorded at Gubbio Piana (GBP) (central Italy), have demonstrated that strong amplification occurs at this accelerometric station, which is installed within an alluvial basin. In particular, Ambraseys et al. [(2005a), Bull Earthq Eng 3:1–53; (2005b), Bull Earth Eng 3:55–73] observed that the strong motion peaks at GBP greatly exceed the median values predicted by the attenuation relationships they derived for Europe. In this work, we analyse and discuss some characteristics of the ground motion recorded at the GBP station. We show that the ground motion parameters, such as peak-ground acceleration and peak-ground velocity, are strongly influenced by the presence of locally induced surface waves that produce both a lengthening of the significant shaking duration and an increase in the peak values with respect to a nearby bedrock site. The basin-induced surface waves are observed in the three components of motion and their effects on the peak values are particularly evident in the vertical component. In the frequency domain, the energy of the surface waves is mostly restricted to the frequency band 0.4–0.8Hz for both the horizontal and vertical components. The horizontal and vertical Fourier amplitudes are also very similar, and this indicates that the H/V spectral ratio technique is not applicable to describing the site response due to the propagation of seismic wave in a complex 2D/3D geological structure. Finally, a preliminary polarization analysis shows that the directions of polarization, as well as the degree of elliptical polarization, exhibit a strong variability with time, that may be related to a complex propagation of Love and Rayleigh waves within the basin.
    Description: Published
    Description: 27-43
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: JCR Journal
    Description: partially_open
    Keywords: site amplification ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 13
    Publication Date: 2017-04-04
    Description: Kostrov's (1974) algorithm for seismic-strain tensor computations, in the version implemented by Wyss et al. (1992a) for error estimates, has been applied to shear-type earthquakes occurring beneath the Etna volcano during 1990-1996. Space-time variations of strain orientations and amplitudes have been examined jointly with ground-deformation and gravimetric data collected in the same period and reported in the literature. Taking also into account the information available from volcanological observations and structural geology, we propose a model assuming that hydraulic pressure by magma emplaced in nearly north-south vertical structures produces the E-W orientation of the maximum compressive strain found in the upper 10 km beneath the crater area. In contrast, regional tectonics deriving from the slow, north-south convergence between the African and European plates appear to play a dominant role in the generation of stress and strain fields at crustal depths deeper than 10 km below the volcano. According to our interpretation, the progressive ascent of magma through the upper crust prior to eruption produces the observed gravity changes, cone inflation and unusual seismic strain rate in the upper 10 km associated with a more sharply defined seismic deformation regime (i.e. very small confidence limits of the epsilon 1 orientation). In agreement with this model, deflation revealed by ground-deformation data during the course of the major 1991-1993 eruption was accompanied by a practically nil level of shallow seismicity.
    Description: Published
    Description: 318-330
    Description: JCR Journal
    Description: open
    Keywords: Mt. Etna ; Italy ; Earthquakes ; Seismic strain ; Stress inversion ; Volcanic processes ; 04. Solid Earth::04.06. Seismology::04.06.08. Volcano seismology
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 14
    Publication Date: 2017-04-04
    Description: A revised Italian strong motion archive has become available since July 2007, including all the records of the strongest events occurred from 1972 to 2004. It contains the uncorrected and corrected accelerograms and the metadata relevant to seismic events, recording stations and instruments added after a careful revision. The availability of this archive allowed us to perform a first step towards an update of the reference ground motion prediction equations for Italy, which were evaluated by Sabetta and Pugliese in (Bull Seismol Soc Am 77:1491–1513, 1987), for peak ground acceleration and velocity, and subsequently extended to the 5% damped pseudovelocity response spectra in 1996. A subset with the 27 major earthquakes occurred in Italy from 1972 to 2002, in the magnitude range 4.6–6.9, was extracted and 235 good quality waveforms were selected, recorded at distances up to 183 km. The goodness of fit of the Sabetta and Pugliese (Bull Seismol Soc Am 86:337–352, 1996) model was explored using two independent statistical approaches (Spudich et al. Bull Seismol Soc Am 89:1156–1170, 1999 and Scherbaum et al. Bull Seismol Soc Am 94:2164– 2185, 2004). The results obtained show that the Sabetta and Pugliese (Bull Seismol Soc Am 77:1491–1513, 1987) does not adequately fit the new strong-motion data set, for its small standard deviation and its non-zero bias. In particular, the most noteworthy result is that the Sabetta and Pugliese (Bull Seismol Soc Am 77:1491–1513, 1987) over-predicts peak ground acceleration and velocity at rock sites. New coefficients for the prediction of horizontal peak ground acceleration, peak ground velocity and acceleration response spectra, adopting the same functional form in Sabetta and Pugliese (Bull Seismol Soc Am 77:1491–1513, 1987), were then evaluated in order to fit the new data set. This paper illustrates the steps made to update the existing ground motion prediction equations for Italy, discusses their limitations and provides the basis for future developments.
    Description: Published
    Description: 591–608
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: JCR Journal
    Description: reserved
    Keywords: ground motion prediction ; equation ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
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  • 15
    Publication Date: 2017-04-04
    Description: The Italian Accelerometic Archive (ITACA) was created in 2007 during a joint project between the Italian Institute for Geophysics and Vulcanology (Istituto Nazionale di Geofisica e Vulcanologia, INGV) and the Italian Civil Protection (Dipartimento della Protezione Civile, DPC). The project, started in 2006, had the aim of filling the data gap of existing strong motion databases and facilitating strong motion data users in obtaining good quality waveforms, through the collection, homogenization and distribution of strong motion data acquired during the period 1972-2004 in Italy by different institutions (Ente Nazionale per l’Energia Elettrica, ENEL, Italian electricity company; Ente per le Nuove tecnologie, l’Energia e l’Ambiente, ENEA, Italian energy and environment organization DPC). The compiled database contains 2182 three-component waveforms generated by 1008 earthquakes with a maximum moment magnitude of 6.9 (1980 Irpinia earthquake) and can be accessed on-line at the portal denominated ITACA at the site http://itaca.mi.ingv.it, where a wide range of search tools enables the user to interactively retrieve events, recording stations and waveforms with particular characteristics, whose parameters can be specified, as needed, through user friendly interfaces. A range of display options allows users to view data in different contexts, extract and download time series and spectral data. This article describes the state of the art up to 2006 and the activities which led to the completion of the project.
    Description: In press
    Description: 5.2. TTC - Banche dati di sismologia strumentale
    Description: JCR Journal
    Description: open
    Keywords: strong-motion ; database ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
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  • 16
    Publication Date: 2017-04-04
    Description: A set of Ground Motion Prediction Equations (GMPEs) for the Italian territory is proposed, exploiting a new strong-motion data set become available since July 2007 through the Italian Accelerometric Archive (ITACA). The data set is composed by 561 three-component waveforms from 107 earthquakes with moment magnitude in the range 4.0–6.9, occurred in Italy from 1972 to 2007 and recorded by 206 stations at distances up to 100 km. The functional form used to derive GMPEs in Italy (Sabetta and Pugliese in Bull Seismol Soc Am 86(2):337–352, 1996) has been modified introducing a quadratic term for magnitude and a magnitude-dependent geometrical spreading. The coefficients for the prediction of horizontal and vertical peak ground acceleration, peak ground velocity and 5% damped acceleration response spectra are evaluated. This paper illustrates the new data set, the regression analysis and the comparisons with recently derived GMPEs in Europe and in the Next Generation Attenuation of Ground Motions (NGA) Project.
    Description: In press
    Description: 4.1. Metodologie sismologiche per l'ingegneria sismica
    Description: JCR Journal
    Description: open
    Keywords: ground motion ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 17
    Publication Date: 2017-04-04
    Description: Large variations of the CO2 flux through the soil were observed between November 2002 and January 2006 at Mt. Etna volcano. In many cases, the CO2 flux was strongly influenced by changes in air temperature and atmospheric pressure. A new filtering method was then developed to remove the atmospheric influences on soil CO2 flux and, at the same time, to highlight the variations strictly related to volcanic activity. Successively, the CO2 corrected data were quantitatively compared with the spectral amplitude of the volcanic tremor by cross correlation function, cross-wavelet spectrum and wavelet coherence. These analyses suggested that the soil CO2 flux variations preceded those of volcanic tremor by about 50 days. Given that volcanic tremor is linked to the shallow (a few kilometer) magma dynamics and soil CO2 flux related to the deeper (*12 km b.s.l.) magma dynamics, the “delayed similarity” between the CO2 flux and the volcanic tremor amplitude was used to assess the average speed in the magma uprising into the crust, as about 170–260 m per day. Finally, the large amount of CO2 released before the onset of the 2004–2005 eruption indicated a deep ingression of new magma, which might have triggered such an eruption.
    Description: In press
    Description: N/A or not JCR
    Description: reserved
    Keywords: Mt. Etna ; Soil CO2 flux ; Volcanic tremor ; Cross-wavelet spectrum ; Wavelet coherence ; Cross correlation function ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring ; 05. General::05.02. Data dissemination::05.02.01. Geochemical data ; 05. General::05.02. Data dissemination::05.02.02. Seismological data
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 18
    Publication Date: 2017-04-04
    Description: Following the 2001 and 2002–2003 flank eruptions, activity resumed at Mt. Etna on 7 September 2004 and lasted for about 6 months. This paper presents new petrographic, major and trace element, and Sr–Nd isotope data from sequential samples collected during the entire 2004–2005 eruption. The progressive change of lava composition allowed defining three phases that correspond to different processes controlling magma dynamics inside the central volcano conduits. The compositional variability of products erupted up to 24 September is well reproduced by a fractional crystallization model that involves magma already stored at shallow depth since the 2002–2003 eruption. The progressive mixing of this magma with a distinct new one rising within the central conduits is clearly revealed by the composition of the products erupted from 24 September to 15 October. After 15 October, the contribution from the new magma gradually becomes predominant, and the efficiency of the mixing process ensures the emission of homogeneous products up to the end of the eruption. Our results give insights into the complex conditions of magma storage and evolution in the shallow plumbing system of Mt. Etna during a flank eruption. Furthermore, they confirm that the 2004–2005 activity at Etna was triggered by regional movements of the eastern flank of the volcano. They caused the opening of a complex fracture zone extending ESE which drained a magma stored at shallow depth since the 2002–2003 eruption. This process favored the ascent of a different magma in the central conduits, which began to be erupted on 24 September without any significant change in eruptive style, deformation, and seismicity until the end of eruption.
    Description: Published
    Description: 781–793
    Description: 2.3. TTC - Laboratori di chimica e fisica delle rocce
    Description: JCR Journal
    Description: reserved
    Keywords: Geochemistry ; Isotopic compositions ; Magma feeding system ; Magma mixing ; Mt. Etna ; 04. Solid Earth::04.08. Volcanology::04.08.03. Magmas ; 04. Solid Earth::04.08. Volcanology::04.08.05. Volcanic rocks
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 19
    Publication Date: 2022-05-24
    Description: Various authors, analysing the set of accelerograms recorded at Gubbio Piana (GBP) (central Italy), have demonstrated that strong amplification occurs at this accelerometric station, which is installed within an alluvial basin. In particular, Ambraseys et al. [(2005a), Bull Earthq Eng 3:1–53; (2005b), Bull Earth Eng 3:55–73] observed that the strong motion peaks at GBP greatly exceed the median values predicted by the attenuation relationships they derived for Europe. In this work, we analyse and discuss some characteristics of the ground motion recorded at the GBP station. We show that the ground motion parameters, such as peak-ground acceleration and peak-ground velocity, are strongly influenced by the presence of locally induced surface waves that produce both a lengthening of the significant shaking duration and an increase in the peak values with respect to a nearby bedrock site. The basin-induced surface waves are observed in the three components of motion and their effects on the peak values are particularly evident in the vertical component. In the frequency domain, the energy of the surface waves is mostly restricted to the frequency band 0.4–0.8Hz for both the horizontal and vertical components. The horizontal and vertical Fourier amplitudes are also very similar, and this indicates that the H/V spectral ratio technique is not applicable to describing the site response due to the propagation of seismic wave in a complex 2D/3D geological structure. Finally, a preliminary polarization analysis shows that the directions of polarization, as well as the degree of elliptical polarization, exhibit a strong variability with time, that may be related to a complex propagation of Love and Rayleigh waves within the basin.
    Description: Published
    Description: JCR Journal
    Description: reserved
    Keywords: Strong motion ; Alluvial basin effects ; Site effects ; Gubbio plain ; 04. Solid Earth::04.06. Seismology::04.06.04. Ground motion
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Mangroves and salt marshes 2 (1998), S. 99-107 
    ISSN: 1572-977X
    Keywords: conductance ; mangrove ; photosynthesis ; productivity ; water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diurnal gas exchange characteristics were measured simultaneously in two mangrove species, Avicennia marina and Bruguiera gymnorrhiza, over 7 d in summer (February–March), to compare their productivity. The study was undertaken in the Beachwood Mangroves Nature Reserve, Durban, South Africa, using fully expanded leaves of young and mature trees at the top of the canopy. Gas exchange was strongly influenced by photosynthetic photon flux density (PPFD), leaf temperature and the accompanying leaf to air vapour pressure deficit (Δ w). Carbon dioxide exchange was saturated at a PPFD of about 600 μmol m-2s-1 in B. gymnorrhiza compared to 800 μmol m-2s-1 in A. marina. Maximal CO2 exchange occurred between 12h00 and 14h00 and was consistently greater in A. marina (8.8 μmol m-2s-1) than in B. gymnorrhiza (5.3 mu;mol m-2s-1). Mean internal CO2 concentrations ( ci) were 260 μl l-1 in A. marina and 252 μl l-1 in B. gymnorrhiza. Photorespiratory activity was 32% in A. marina and 30% in B. gymnorrhiza. Mean water use efficiency (WUE) was 8.0 μmol mmol-1 in A. marina and 10.6 μmol mmol-1 in B. gymnorrhiza. Diurnal leaf water potentials ranged from –0.8 to –3.5 MPa and were generally lower in A. marina.
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  • 21
    Electronic Resource
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    Springer
    Mangroves and salt marshes 2 (1998), S. 191-198 
    ISSN: 1572-977X
    Keywords: canopy ; Hinchinbrook ; leaf area index ; mangrove ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Data on stand structure and rates of photosynthesis were used to estimate net canopy carbon fixation and carbon accumulation as living biomass in mangrove forests in Hinchinbrook Channel, Australia. Total annual canopy net carbon fixation was estimated to be about 29 t C ha−1 yr−1. This equates to about 204,000 t C yr−1 for all mangrove forests in Hinchinbrook Channel. Of this, only about 12% was stored as living plant biomass. Although it is not yet possible to present a robust carbon balance for mangrove trees, the remainder is presumably lost through plant respiration, litter fall, root turnover and exudation of organic compounds from roots.
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  • 22
    Electronic Resource
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    Springer
    Mangroves and salt marshes 3 (1999), S. 147-153 
    ISSN: 1572-977X
    Keywords: conductance ; gas exchange ; mangrove ; photorespiration ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthetic characteristics were investigated in the geographically isolated and restricted mangrove species, P.rhizophoreae. Gas exchange measurements were made on two to seven years old hydroponically grown plants maintained in 10%, 50% and 100% seawater. CO2 exchange in the 50% and 100% seawater treatments was reduced by 10% and 26%, respectively, compared to the 10% seawater treatment. CO2 response curves indicated that carboxylation efficiency was greater in 10% than in 50% seawater, while stomatal limitation increased from 11% to 16% as salinity increased from 10% to 50% seawater. Carbon losses via photorespiration (31% and 41%) and CO2 compensation point (67 and 81 μ11−1) were greater in 50% than in the 10% seawater treatment. Maximal CO2 exchange occurred at 30 °C with no differences among the salinity treatments. The results indicate that P. rhizophoreae exhibits many gas exchange characteristics previously reported for other mangroves.
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  • 23
    ISSN: 1432-1939
    Keywords: Key words Agrostis canina ; CO2 vents ; photosynthesis ; lignification ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The aim of this study was to characterise growth and photosynthetic capacity in plants adapted to long-term contrasting atmospheric CO2 concentrations (C a). Seeds of Agrostis canina L. ssp. monteluccii were collected from a natural CO2 transect in central-western Italy and plants grown in controlled environment chambers at both ambient and elevated CO2 (350 and 700 μmol mol−1) in nutrient-rich soil. Seasonal mean C a at the source of the plant material ranged from 610 to 451 μmol CO2 mol−1, derived from C4 leaf stable carbon isotope discrimination (δ13C). Under chamber conditions, CO2 enrichment stimulated the growth of all populations. However, plants originating from elevated C a exhibited higher initial relative growth rates (RGRs) irrespective of chamber CO2 concentrations and a positive relationship was found between RGR and C a at the seed source. Seed weight was positively correlated with C a, but differences in seed weight were found to explain no more than 34% of the variation in RGRs at elevated CO2. Longer-term experiments (over 98 days) on two populations originating from the extremes of the transect (451 and 610 μmol CO2 mol−1) indicated that differences in growth between populations were maintained when plants were grown at both 350 and 700 μmol CO2 mol−1. Analysis of leaf material revealed an increase in the cell wall fraction (CWF) in plants grown at elevated CO2, with plants originating from high C a exhibiting constitutively lower levels but a variable response in terms of the degree of lignification. In vivo gas exchange measurements revealed no significant differences in light and CO2 saturated rates of photosynthesis and carboxylation efficiency between populations or with CO2 treatment. Moreover, SDS-PAGE/ LISA quantification of leaf ribulose bisphosphate carboxylase/oxygenase (Rubisco) showed no difference in Rubisco content between populations or CO2 treatments. These findings suggest that long-term adaptation to growth at elevated CO2 may be associated with a potential for increased growth, but this does not appear to be linked with differences in the intrinsic capacity for photosynthesis.
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  • 24
    Electronic Resource
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    Water, air & soil pollution 85 (1995), S. 1461-1466 
    ISSN: 1573-2932
    Keywords: ozone ; wheat ; Triticum aestivum ; growth ; senescence ; biomass partitioning ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In closed-chamber fumigation experiments dry matter partitioning and chlorophyll fluorescence of wheat were studied, analysing the effects of ozone during different stages of plant development. Ozone causes enhanced leaf senescence, leading to a loss of green leaf area and, consequently to a decreased supply of assimilates, affecting (in increasing order of severeness) stem, ear and grain productivity because of reduced storage pools for translocation. Leaves of plants before shooting stage were most sensitive but the lack of green leaf area after ear emergence had the most pronounced effects on grain yield. Measurements of photochemical capacity showed that evidence for negative ozone effects could be found in changes of chlorophyll fluorescence parameters in leaf sections not yet showing visible ozone injury. Negative effects on photosynthesis were more distinct with increasing accumulated ozone dose, with increasing age of leaf tissue and with increasing ozone sensitivity of the cultivar. The changes in chlorophyll fluorescence are most likely to be explained by a decreased pool size of plastoquinones caused by ozone.
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  • 25
    ISSN: 1573-5028
    Keywords: Cell cycle ; circadian clock ; green alga ; GTP-binding proteins ; light regulation ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ADP-ribosylation factor (ARF) is a highly conserved, low molecular mass (ca. 21 kDa) GTP-binding protein that has been implicated in vesicle trafficking and signal transduction in yeast and mammalian cells. However, little is known of ARF in plant systems. A putative ARF polypeptide was identifed in subcellular fractions of the green alga Chlamydomonas reinhardtii, based on [32P]GTP binding and immunoblot assays. A cDNA clone was isolated from Chlamydomonas (Arf1), which encodes a 20.7 kDa protein with 90% identity to human ARF1. Northern blot analyses showed that levels of Arf1 mRNA are highly regulated during 12 h/12 h light/dark (LD) cycles. A biphasic pattern of expression was observed: a transient peak of Arf1 mRNA occurred at the onset of the light period, which was followed ca. 12 h later by a more prominent peak in the early to mid-dark period. When LD-synchronized cells were shifted to continuous darkness, the dark-specific peak of Arf1 mRNA persisted, indicative of a circadian rhythm. The increase in Arf1 mRNA at the beginning of the light period, however, was shown to be light-dependent, and, moreover, dependent on photosynthesis, since it was prevented by DCMU. We conclude that the biphasic pattern of Arf1 mRNA accumulation during LD cycles is due to regulation by two different factors, light (which requires photosynthesis) and the circadian clock. Thus, these studies identify a novel pattern of expression for a GTP-binding protein gene.
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  • 26
    ISSN: 1573-5028
    Keywords: gene expression ; photosynthesis ; protein turnover ; psbA ; tac promoter ; D1 protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Over-expression of the psbAIII gene encoding for the D1 protein (form II; D1:2) of the photosystem II reaction centre in the Synechococcus sp. PCC 7942 was studied using a tac promoter and the lacI Q system. Over-expression was induced with 40 μg/ml IPTG in the growth medium for either 6 or 12 h at growth irradiance (50 μmol photons m-2 s-1). This treatment doubled the amount of psbAII/III mRNA and the D1:2 protein in membranes but decreased the amount of psbAI messages and the D1:1 protein. The total amount of both heterodimeric reaction centre proteins, D1 and D2, remained constant under growth light conditions, indicating that the number of PSII centres in the membranes was not affected, only the form of the D1 protein was changed from D1:1 to D1:2 in most centres. When the cells were photoinhibited either at 500 or 1000 μmol photons m-2 s-1, in the presence or absence of the protein synthesis inhibitor lincomycin, the D1:2 protein remained at a higher level in cells in which over-expression had been induced by IPTG. These cells were also less prone to photoinhibition of PSII. It is suggested that the tolerance of cells to photoinhibition increases when most PSII reaction centres contain the D1:2 protein at the beginning of high irradiance. This tolerance is further strengthened by maintaining psbAIII gene over-expression during the photoinhibitory treatment.
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  • 27
    ISSN: 1573-5028
    Keywords: antisense ; Corynebacterium glutamicum ; Escherichia coli ; Flaveria trinervia ; overexpression ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phosphoenolpyruvate carboxylase (PEPC) genes from Corynebacterium glutamicum (cppc), Escherichia coli (eppc) or Flaveria trinervia (fppc) were transferred to Solanum tuberosum. Plant regenerants producing foreign PEPC were identified by Western blot analysis. Maximum PEPC activities measured in eppc and fppc plants grown in the greenhouse were doubled compared to control plants. For cppc a transgenic plant line could be selected which exhibited a fourfold increase in PEPC activity. In the presence of acetyl-CoA, a known activator of the procaryotic PEPC, a sixfold higher activity level was observed. In cppc plants grown in axenic culture PEPC activities were even higher. There was a 6-fold or 12-fold increase in the PEPC activities compared to the controls measured in the absence or presence of acetyl-CoA, respectively. Comparable results were obtained by transient expression in Nicotiana tabacum protoplasts. PEPC of C. glutamicum (PEPC C.g.) in S. tuberosum leaf extracts displays its characteristic K m(PEP) value. Plant growth was examined with plants showing high expression of PEPC and, moreover, with a plant cell line expressing and antisense S. tuberosum (anti-sppc) gene. In axenic culture the growth rate of a cppc plant cell line was appreciably diminished, whereas growth rates of an anti-sppc line were similar or slightly higher than in controls. Malate levels were increased in cppc plants and decreased in antisense plants. There were no significant differences in photosynthetic electron transport or steady state CO2 assimilation between control plants and transformants overexpressing PEPC C.g. or anti-sppc plants. However, a prolonged dark treatment resulted in a delayed induction of photosynthetic electron transport in plants with less PEPC. Rates of CO2 release in the dark determined after a 45 min illumination period at a high proton flux density were considerably enhanced in cppc plants and slightly diminished in anti-sppc plants. When CO2 assimilation rates were corrected for estimated rates of mitochondrial respiration in the light, the electron requirement for CO2 assimilation determined in low CO2 was slightly lower in transformants with higher PEPC, whereas transformants with decreased PEPC exhibited an appreciably elevated electron requirement. The CO2 compensation point remained unchanged in plants (cppc) with high PEPC activity, but might be increased in an antisense plant cell line. Stomatal opening was delayed in antisense plants, but was accelerated in plants overexpressing PEPC C.g. compared to the controls.
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  • 28
    ISSN: 1573-5028
    Keywords: photosystem II ; photosynthesis ; chlorophyll-binding protein ; Synechocystis ; oxygen evolution ; oligonucleotide-mediated mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract CP 47, a component of photosystem II (PSII) in higher plants, algae and cyanobacteria, is encoded by the psbB gene. Site-specific mutagenesis has been used to alter a portion of the psbB gene encoding the large extrinsic loop E of CP 47 in the cyanobacterium Synechocystis 6803. Alteration of a lysine residue occurring at position 321 to glycine produced a strain with altered PSII activity. This strain grew at wild-type rates in complete BG-11 media (480 µM chloride). However, oxygen evolution rates for this mutant in complete media were only 60% of the observed wild-type rates. Quantum yield measurements at low light intensities indicated that the mutant had 66% of the fully functional PSII centers contained in the control strain. The mutant proved to be extremely sensitive to photoinactivation at high light intensities, exhibiting a 3-fold increase in the rate of photoinactivation. When this mutant was grown in media depleted of chloride (30 µM chloride), it lost the ability to grow photoautotrophically while the control strain exhibited a normal rate of growth. The effect of chloride depletion on the growth rate of the mutant was reversed by the addition of 480 µM bromide to the chloride-depleted BG-11 media. In the presence of glucose, the mutant and control strains grew at comparable rates in either chloride-containing or chloride-depleted media. Oxygen evolution rates for the mutant were further depressed (28% of control rates) under chloride-limiting conditions. Addition of bromide restored these rates to those observed under chloride-sufficient conditions. Measurements of the variable fluorescence yield indicated that the mutant assembled fewer functional centers in the absence of chloride. These results indicate that the mutation K321G in CP 47 affects PSII stability and/or assembly under conditions where chloride is limiting.
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  • 29
    ISSN: 1573-5028
    Keywords: chloroplasts ; gene expression ; heat bleaching ; photosynthesis ; transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A shift in the ratio of chlorophyll (Chl) a and Chl b is an early indicator of heat bleaching in Euglena gracilis. This observation prompted us to consider whether or not changes in steady-state levels of chloroplast transcripts and in transcriptional activity could limit the synthesis of Chl a-binding proteins in bleaching plastids. We found that the mature transcripts for CP47 and CP43, the Chl a-binding apoproteins of the proximal antenna of photosystem II, decline sharply very early during bleaching. Our study also shows that transcription of psbB and psbC, the chloroplast genes encoding CP47 and CP43, remains essentially unchanged during the same interval. We conclude that posttranscriptional events, such as mRNA stability, could play a major role in initiating an irreversible loss of chloroplast function in Euglena at a moderately elevated temperature. Lack of these transcripts would eventually impair the assembly of photosystem II in thylakoids.
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  • 30
    ISSN: 1573-5028
    Keywords: Cyanobacteria ; gene copy number ; light regulation ; photosynthesis ; photosystem II reaction center ; polymerase chain reaction ; psbA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA sequence, copy number, expression and phylogenetic relevance of the psbA gene from the abundant marine prokaryote P. marinus CCMP 1375 was analyzed. The 7 amino acids near the C-terminus missing in higher plant and in Prochlorothrix hollandica D1 proteins are present in the derived amino acid sequence. P. marinus contains only a single psbA gene. Thus, this organism lacks the ability to adapt its photosystem II by replacement of one type of D1 by another, as several cyanobacteria do. Phylogenetic trees suggested the D1-1 iso-form from Synechococcus PCC 7942 as the next related D1 protein and place P. Marinus separately from Prochlorothrix hollandica among the cyanobacteria.
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  • 31
    ISSN: 1573-5028
    Keywords: chlorophyll synthesis ; cyanobacteria ; chlorophyl-binding proteins ; photosynthesis ; thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Part of the chlL gene encoding a component involved in light-independent protochlorophyllide reduction was deleted in wild type and in a photosystem I-less strain of Synechocystis sp. PCC 6803. In resulting mutants, chlorophyll biosynthesis was fully light-dependent. When these mutants were propagated under light-activated heterotrophic growth conditions (in darkness except for 15 min of weak light a day) for several weeks, essentially no chlorophyll was detectable but protochlorophyllide accumulated. Upon return of the chlL - mutant cultures to continuous light, within the first 6 h chlorophyll was synthesized at the expense of protochlorophyllide at a rate independent of the presence of photosystem I. Chlorophyll biosynthesized during this time gave rise to a 685 nm fluorescence emission peak at 77 K in intact cells. This peak most likely originates from a component different from those known to be directly associated with photosystems II and I. Development of 695 and 725 nm peaks (indicative of intact photosystem II and photosystem I, respectively) required longer exposures to light. After 6 h of greening, the rate of chlorophyll synthesis slowed as protochlorophyllide was depleted. In the chlL - strain, greening occurred at the same rate at two different light intensities (5 and 50 μE m-2s-1), indicating that also at low light intensity the amount of light is not rate-limiting for protochlorophyllide reduction. Thus, in this system the rate of chlorophyll biosynthesis is limited neither by biosynthesis of photosystems nor by the light-dependent protochlorophyllide reduction. We suggest the presence of a chlorophyll-binding ‘chelator’ protein (with 77 K fluorescence emission at 685 nm) that binds newly synthesized chlorophyll and that provides chlorophyll for newly synthesized photosynthetic reaction centers and antennae.
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  • 32
    ISSN: 1573-5028
    Keywords: Cyanobacteria ; photosynthesis ; random mutagenesis ; sodium bisulfite ; thylakoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To identify amino acid residues of the D2 protein that are critical for functional photosystem II (PS II), sodium bisulfite was utilized for in vitro random mutagenesis of the psbDI gene from Synechocystis sp. PCC 6803. Sodium bisulfite reacts specifically with cytosine in single-stranded regions of DNA and does not attack double-stranded DNA. Using a hybrid plasmid that was single-stranded in the region to be mutagenized and that was double-stranded elsewhere, mutations were targeted to a specific psbDI region coding for the lumenal A-B loop of the D2 protein. Several mutants were isolated with a total of 15 different amino acid changes in the loop. The majority of these mutations did not result in a loss of photoautotrophic growth or in significantly altered PS II function. However, mutation of Glu-69 to Lys, Ser-79 to Phe, and Ser-88 to Phe were found to influence photosystem II activity; the importance of the latter two residues for proper PS II function was unexpected. Cells carrying the double mutation S79F/S88F in D2 did not grow photoautotrophically and had no functionally active PS II centers. The single mutant S79F was also incapable of photoautrophic growth, but displayed reasonably stable oxygen evolution, while PS II function in the single mutant S88F appeared to be close to normal. Because of the more pronounced phenotype of the S79F/S88F strain as compared to the single mutants, both Ser residues appear to affect stable assembly and function of the PS II complex. The mechanism by which the S79F mutant loses photoautotrophic growth remains to be established. However, these results show the potential of targeted random mutagenesis to identify functionally important residues in selected regions of proteins.
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  • 33
    ISSN: 1871-4528
    Keywords: benzylaminopurine ; gibberellic acid ; senescence ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The modifying effects of applying the plant growth regulators (PGRs) benzylaminopurine (BAP), gibberellic acid (GA3) and BAP+GA3 on physiological age were studied. Two experiments with two cultivars, differing in rate of physiological ageing (medium-early Pampeana, medium-late Huinkul) and two storage systems were performed during 1988/89 and 1989/90 in two different potato areas of Argentina. In both seasons seed tubers stored in heaps reached an advanced physiological age at planting, compared with tubers from the cold store. Seed tubers of cv. Pampeana were older than those of Huinkul. compared with control crops, those sprayed with BAP maintained ground cover and photosynthesis for longer, and those sprayed with GA3 for a shorter period. Consequently tuber yield was decreased by GA3 in 1988/89, but in 1989/90 all crops treated with PGRs outyielded the control. BAP could overcome effects of advanced physiological age on crop senescence and tuber yield.
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  • 34
    ISSN: 1871-4528
    Keywords: water stress ; photosynthesis ; stress index ; dry matter partitioning ; tuber quality ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A lysimeter experiment was performed to study the optimal allocation of limited water supply in potatoes. Irrigation regimes equal to 40, 60 and 80% of maximum evapotranspiration (ET) were evenly applied over the crop cycle. Other treatments involved withholding 80 mm of irrigation, based on ET, beginning at each of three designated growth stages (tuber initiation, early and late tuber growth). An irrigated control treatment, restoring the entire ET, was included for comparison. Continuous drought stress reduced photosynthesis as irrigation volumes were reduced. Plant biomass and tuber yield decreased almost proportionally to water consumption, so that WUE was roughly constant. N uptake was highest in the control and in 80% ET treatment. Withholding water during tuberisation severely hindered plant physiological processes and penalized tuber yield. Reductions in photosynthesis, total biomass and yield were the greatest when drought was imposed during tuber initiation. The earliest stress resulted in the lowest WUE and N uptake. A new crop water stress index (SI) was proposed, which combines atmospheric demand for water and canopy temperature.
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  • 35
    ISSN: 1610-7403
    Keywords: Chamaecyparis obtusa Endl. ; photosynthesis ; Pinus densiflora Sieb. et Zucc. ; stomatal conductance ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was conducted to investigate the potential for modifying drought tolerance of Japanese cypress (Chamaecyparis obtusa Endl.) and Japanese red pine (Pinus densiflora Sieb. et Zucc.). Three-year-old seedlings were controlled for five-months at three different soil water potentials ({ie73-1}). Japanese cypress exposed to high {ie73-2} was able to maintain higher photosynthesis (Phn), transpiration (Tr) and stomatal conductance to H2O (gH2O) in comparison to low {ie73-3} pretreatments, however, there was no significant difference in Phn for Japanese red pine. Soil water potential at the threshold from the maximum to limited Phn was higher in high {ie73-4} pretreatments than in low {ie73-5} pretreatments. Net photosynthesis, Tr and gH2O decreased more rapidly in high {ie73-6} pretreatments than in low {ie73-7} pretreatments. Transpiration decreased more significantly than Phn, thus, resulted in increased water use efficiency. All these factors are likely to result in significant improvements in the drought tolerance. Japanese red pine seems more drought-tolerant than Japanese cypress. Japanese cypress is suitable to soil of −0.05 MPa water potential, and Japanese red pine is suitable to −0.16 MPa and even dryer soils.
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  • 36
    ISSN: 1610-7403
    Keywords: chlorophyll fluorescence ; electron transport ; photorespiration ; photosynthesis ; quantum yield ; tree
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using attached and detached leaves ofAcer palmatum Thunb. andRhaphiolepsis umbellata Makino, pulse-modulated chlorophyll fluorescence and CO2 exchange were measured. Quantum yield of photosynthesis was determined from the fluorescence parameter(Fm′−Fs)/Fm′, where (Fm′−Fs) was defined as the difference between steady state chlorophyll fluorescence (Fs) and maximum fluorescence (Fm′) elicited by a saturating light pulse. The rate of electron transport through photosystem II (total electron flow) was calculated from the product of quantum yield andA (PFD), whereA is the rate of absorbed photons as given by leaf absorptance, and PFD is the photon flux density at the leaf surface. The rate of electron transport dependant on CO2 uptake (assimilative electron flow) was calculated from the gross photosynthetic rate in a leaf. The difference between the rates of total and assimilative electron transport was denoted as the rate of non-assimilative electron transport which depends on photorespiration and oxygen reduction. Available data provided quantitative information on the rate of non-assimilative electron flow in intact leaves. When leaf photosynthesis ofA. palmatum was measured under sunlight, the rates of total and assimilative electron transport were determined to be approximately 900 and 150 μmol equiv. e/mg Chl·h, respectively. The difference (750 μmol equiv. e/mg Chl·h) was attributed to the activity of non-assimilative electron flow. The ratio of total to assimilative electron flow was found to increase gradually with rising in irradiance. The results suggest that non-assimilative electron flow occurred at much higher rate than assimilative electron flow at high irradiance. Implications of the results are briefly discussed in relation to photosynthesis limitation in tree leaves.
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  • 37
    ISSN: 1573-5125
    Keywords: chlorophyll fluorescence ; nutrient limitation ; phytoplankton ; photosynthesis ; quantum efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 38
    ISSN: 1573-5125
    Keywords: intertidal areas ; photosynthetically active radiation ; photosynthesis ; Tagus estuary ; tides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthetically active radiation (PAR) and temperature were measured continuously at the surface of estuarine intertidal sediments in the Tagus estuary, Portugal, along two spring-neap tidal cycles. PAR and temperature were strongly conditioned by the periodic tidal inundation, with large and abrupt variations occurring during flooding and ebbing. PAR levels reaching the sediment surface decreased very rapidly to zero or very low values during most of the daytime immersion. Inundation during high tide had the general effect of attenuating the amplitude of daily temperature fluctuation, with the incoming water usually warmer than the sediment during the night or early morning and cooler during the day. The daily progression of tidal emersion resulted in a clear fortnightly variation in total daily PAR reaching the sediment surface, while both daily mean temperature and mean temperature of diurnal low tide periods failed to exhibit a well-defined fortnightly periodicity. The obtained results indicate that the estuarine intertidal environment is dominated, at sub-seasonal time scales, by fortnightly periodicity in irradiance and temperature conditions favourable for benthic photosynthesis.
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  • 39
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    Journal of applied phycology 10 (1998), S. 547-554 
    ISSN: 1573-5176
    Keywords: herbicide ; green alga ; growth ; nutrients ; photosynthesis ; it Protosiphon botryoides ; respiration ; Thiobencarb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of the herbicide thiobencarb (Saturn) were tested on the growth and physiology of the chlorophyte Protosiphon botryoides isolated from an Egyptian paddy. Assays were conducted using 16-day batch cultures. Chlorophyll and dry weight biomass yields were significantly reduced at 2–3 mg L-1 thiobencarb, and dark respiration increased and protein decreased significantly at 3 mg L-1. Reductions in exponential specific growth rate (μ) were generally small, but in some cases significant. Thiobencarb also slightly, but significantly, reduced the 77 K fluorescence parameter Fv/Fm, an indicator of maximum photosynthetic efficiency. No consistent dose-dependent changes occurred in chlorophyll per unit dry weight, total carbohydrate or gross photosynthetic capacity. Whereas half of the added thiobencarb was recovered from control (uninoculated) medium, it was largely absent from cells and culture medium after sixteen days, indicating biodegradation by the alga or associated bacteria. P. botryoides recovered fully within sixteen days following subculture in thiobencarb-free medium. Independently varying phosphate and nitrate nine-fold had no clear effect on the sensitivity of P. botryoides to thiobencarb.
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  • 40
    ISSN: 1573-5176
    Keywords: Cyanobacterium ; Spirulina platensis ; Arthrospira ; CO2 ; organic carbon ; nitrogen ; photosynthesis ; batch culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The consequences of the addition of CO2 (1%) in cultures of S. platensis are examined in terms of biomass yield, cell composition and external medium composition. CO2 enrichment was tested under nitrogen saturating and nitrogen limiting conditions. Increasing CO2 levels did not cause any change in maximum growth rate while it decreased maximum biomass yield. Protein and pigments were decreased and carbohydrate increased by high CO2, but the capability to store carbohydrates was saturated. C:N ratio remained unchanged while organic carbon released to the external medium was enhanced, suggesting that organic carbon release in S. platensis is an efficient mechanism for the maintenance of the metabolic integrity, balancing the cell C:N ratio in response to environmental CO2 changes. CO2 affected the pigment content: Phycocyanin, chlorophyll and carotenoids were reduced in around 50%, but the photosynthetic parameters were slightly changed. We propose that in S. platensis CO2 could act promoting degradation of pigments synthetised in excess in normal CO2 conditions, that are not necessary for light harvesting. Nitrogen assimilation was significantly not affected by CO2, and it is proposed that the inability to stimulate N assimilation by CO2 enrichment determined the lack of response in maximum growth rate.
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  • 41
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    Journal of applied phycology 11 (1999), S. 195-201 
    ISSN: 1573-5176
    Keywords: dense algal suspension ; light-harvesting pigment ; photosynthesis ; productivity ; cyanobacterium ; Synechocystis PCC 6714
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microalgal productivity was examined using both a wild type and a phycocyanin-deficient mutant of Synechocystis PCC 6714 (PD-1). The culture was conducted at various light intensities under low and high cell densities in a continuous culture system. At low light intensity, photosynthetic productivity was almost the same for both low and high cell densities. However, at higher light intensities photosynthetic productivity was higher in mutant PD-1 than in the wild type. At 2000 μmol photon m−2 s−1 the productivity was 50% higher in mutant PD-1. This result is consistent with our first report (Nakajima & Ueda, 1997), which showed that photosynthetic productivity can be improved by reducing the light harvesting pigment content in high cell density cultures at high light intensities. It is concluded that the technology for reducing LHP content is a useful method for improving photosynthetic productivity in algal mass production.
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  • 42
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    Journal of applied phycology 8 (1996), S. 263-273 
    ISSN: 1573-5176
    Keywords: molecular biology ; mutagenesis ; photosynthesis ; protein engineering ; respiration ; thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cyanobacteriumSynechocystis sp. PCC 6803 is readily amenable to targeted mutagenesis: Foreign DNA is taken up spontaneously, and after uptake DNA can be integrated into the organism's genome by homologous recombination. Using appropriate DNA constructs for transformation, specific genes in the organism can be interrupted, deleted, or replaced by modified gene copies. The organism can grow under a number of different conditions, ranging from photoautotrophic to fully heterotrophic modes, making genetic modifications that alter fundamental processes such as photosynthesis and/or respiration feasible. For example, deletion of photosystem I leads to an obligate (photo)heterotrophic strain in which photosystem II-generated electrons appear to be consumed by respiratory processes, whereas deletion of photosystem II leads to an obligate (photo)heterotrophic strain in which cyclic electron flow around photosystem I appears to remain active. A major advantage ofSynechocystis sp. PCC 6803 is that its entire genome has been sequenced (by S. Tabata and co-workers), opening many avenues to address basic and applied research problems. For example, genes can be introduced, modified or deleted, and hypotheses regarding the function of an open reading frame can be tested by deletion of this open reading frame. Methods to modify genes are numerous. In addition to site-directed mutagenesis, novel molecular genetic approaches including ‘targeted random mutagenesis’, combinatorial mutagenesis and introduction of hybrid genes have come of age and have proven to be very powerful tools in protein engineering. These approaches have been utilized primarily in this strain to study photosynthesis, but applications of this technology, including pathway engineering, alterations of substrate specificity of enzymes and introduction of tolerance to a variety of stresses, are equally feasible in relation to more applied aims. For optimal utilization of the potential of theSynechocystis sp. PCC 6803 system, however, an increased emphasis toward understanding the biochemistry and molecular physiology of cyanobacteria will also be critically important.
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  • 43
    ISSN: 1573-5176
    Keywords: fluorescence ; photoinhibition ; photosynthesis ; Spirulina ; photobioreactor ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A chlorophyll fluorescence technique was applied to anin situ study on the effects of low temperature and high light stresses onSpirulina cultures grown outdoors in controlled tubular photobioreactors at high (1.1 g L−1) and low (0.44 g L−1) biomass concentrations. Diurnal changes in PSII photochemistry (F v/F m) after 15 min of darkness, or in the light (dF/F′ m), and non-photochemical (qN) quenching were measured using a portable, pulse-amplitude-modulated fluorometer. The depression of theF v/F m ratio ofSpirulina cultures grown outdoors at 25°C (i.e. 10°C below optimum for growth) and 0.44 g L−1, reached 30% at the middle of the day. At the same time of the day thedF/F′ m ratio showed a reduction of up to 52%. The depression of bothF v/F m anddF/F′ m was lower in the cultures grown at 1.1 g L−1. Photoinhibition reduced the daily productivity of the culture grown at 0.44 g L−1 and 25°C by 33% with respect to that grown at 35°C. Changes in the growth yields of the cultures grown under different temperatures and growth rates correlate well with analogous changes in photon yield (dF/F′ m). Simple measurements of photochemical yield (F v/F m) can be used to test the physiological status ofSpirulina cultures. The results indicate that the saturating pulse fluorescence technique, when usedin situ, is a powerful tool for assessment of the photosynthetic characteristics of outdoor cultures ofSpirulina.
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  • 44
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    Journal of applied phycology 10 (1998), S. 419-425 
    ISSN: 1573-5176
    Keywords: Gracilaria cornea ; photosynthesis ; respiration ; chlorophyll ; phycoerythrin ; Florida ; salinity ; temperature ; irradiance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The agarophyte Gracilaria cornea, collected over 2.5 y in the Florida Keys, shows adaptations to oceanic salinities and subtropical to tropical water temperatures in its photosynthetic and respiratory responses as measured with a respirometer. No seasonal pattern in responses to irradiance, temperature, and salinity were evident between five collections over a 20-month period, indicating the tropical nature of the populations from Bahia Honda and Pigeon Keys. Concentrations of chlorophyll a (0.09 to 0.41 mg g d wt-1) and phycoerythrin (0.06 to 0.36 mg g d wt- 1) were low and reflect the low nutrient regime of the habitats, especially when compared to laboratory cultured plants. Compensation and saturation irradiances were also low (11–38 and 90–127 μmol photon m-2 s-1), indicating acclimation to lower irradiances in their shallow (1–2 m depth) habitats where turbidity can be high. In comparison with other subtropical and warm temperate species of Gracilaria, G. cornea had lower levels of pigment, but similarly high photosynthetic efficiency, demonstrating shade adaptation; it had only limited tolerance to salinities below 20‰ and temperatures below 15 °C. Thus, G. cornea from the Florida Keys in mariculture would require subtropical to tropical temperatures and stable oceanic salinities.
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  • 45
    ISSN: 1573-5176
    Keywords: Chlorophyll antenna size ; damage and repair cycle ; photon use efficiency ; photosynthesis ; photoinhibition ; Dunaliella salina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photon use efficiencies and maximal rates of photosynthesis in Dunaliella salina (Chlorophyta) cultures acclimated to different light intensities were investigated. Batch cultures were grown to the mid-exponential phase under continuous low-light (LL: 100 μmol photon m-2 s-1) or high-light (HL: 2000 μmol photon m-2 s-1) conditions. Under LL, cells were normally pigmented (deep green) containing ∼500 chlorophyll (Chl) molecules per photosystem II (PSII) unit and ∼250 Chl molecules per photosystem I (PSI). HL-grown cells were yellow-green, contained only 60 Chl per PSII and 100 Chl per PSI and showed signs of chronic photoinhibition, i.e., accumulation of photodamaged PSII reaction centers in the chloroplast thylakoids. In LL-grown cells, photosynthesis saturated at ∼200 μmol photon m-2 s-1 with a rate (Pmax) of ∼100 mmol O2 (mol Chl)-1 s-1. In HL-grown cells, photosynthesis saturated at much higher light intensities, i.e. ∼2500 μmol photon m-2 s-1, and exhibited a three-fold higher Pmax (∼300 mmol O2 (mol Chl)-1 s-1) than the normally pigmented LL-grown cells. Recovery of the HL-grown cells from photoinhibition, occurring prior to a light-harvesting Chl antenna size increase, enhanced Pmax to ∼675 mmol O2 (mol Chl)-1 s-1. Extrapolation of these results to outdoor mass culture conditions suggested that algal strains with small Chl antenna size could exhibit 2–3 times higher productivities than currently achieved with normally pigmented cells.
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  • 46
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    Journal of applied phycology 11 (1999), S. 157-163 
    ISSN: 1573-5176
    Keywords: Euglena gracilis ; photosynthesis ; waste water ; pulp and paper industry ; ultraviolet-B radiation (280–320 nm) ; pentachlorophenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The green flagellate Euglena gracilis has been used as a model organism to elucidate the possible large-scale and short-term effects of waste substances from the pulp and paper industry on photosynthetic efficiency (PE). Different concentrations of waste substances before and after treatment in a cleaning system were studied. The uncleaned sample at concentrations up to 1:10 and the cleaned sample at concentrations up to 1:5 showed stimulating effects on the PE after 7 days of incubation compared to the control. The effects of waste substances on the PE of E. gracilis were also studied in combination with short-term studies (20 and 40 min) of ultraviolet-B radiation (UV-B, 280–320 nm). It was shown that increasing concentrations of the uncleaned sample had continuously stimulating effects on the PE and worked protectively against UV-B radiation. The cleaned sample exhibited no effects, or negative effects, on the PE of E. gracilis together with UV-B radiation compared to the experiments with only UV-B radiation. At the concentration 1:1 of the cleaned sample an increase in the PE was detected due to the high concentration of the coloured substances and a decrease in the UV-B penetration. PE revealed itself to be highly sensitive for detecting toxic effects on E. gracilis and is thus very promising for use in regular toxicity tests of waste water from pulp and paper industry.
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  • 47
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    Journal of applied phycology 10 (1998), S. 51-53 
    ISSN: 1573-5176
    Keywords: blue-green alga ; cyanobacterium ; Fv/Fmlight ; Nostoc flagelliforme ; photosynthesis ; rewetting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract PS II photochemical efficiency (Fv/Fm) of Nostoc flagelliforme was examined after rewetting in order to investigate the light-dependency of its photosynthetic recovery. Fv/Fm was not detected in the dark, but was immediately recognized in the light. Different levels of light irradiation (4, 40 and 400 µmol photon m2 s-1) displayed different effects on the recovery process of photosynthesis. The intermediate level led to the best recovery of photochemical efficiency; the low light required longer and the high light inhibited the extent of the recovered efficiency. It was concluded that the photosynthetic recovery of N. flagelliforme is both light-dependent and influenced by photon flux density.
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  • 48
    ISSN: 1573-5176
    Keywords: ammonium ; C:N ratio ; tank culture ; dietary fibre ; fatty acids ; nitrogen ; photosynthesis ; Ulva rigida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Physiological and biochemical changes in relation to inorganic nitrogen availability were studied for tank-cultivated Ulva rigida grown under nitrogen- enriched and nitrogen-depleted seawater. U. rigida was initially cultivated in nitrogen-enriched seawater (daily concentrations of NH4+ and NO3- + NO2- ranged between 0.5–1.7 and 0.06–0.15 mg L-1, respectively), then transferred to nitrogen-depleted seawater where photosynthetic capacity decreased to zero after 23 d. At the time (14 d) when photosynthetic rates were lower than 2.0 μmol O2 g-1 FW min-1 and strong bleaching had occurred, some algae were returned to the initial nitrogen-enriched seawater to study recovery from N-limited growth. Data on biochemical composition (chlorophylls, ash, caloric content, fatty acids and dietary fibres) and colouration varied significantly depending on the nitrogen conditions. C:N ratios correlated significantly with biochemical parameters. Fatty acid (FA) synthesis continued during the N-starvation period; saturated and mono-unsaturated FA increased to a maximun of 72.2%, while poly-unsaturated fatty acids (PUFA) decreased to 27.7%. During the N-enriched recovery period, the reverse was found. C:N ratios above 10 correlated with carbohydrate synthesis as shown by the dietary fibre level. Under nitrogen enriched conditions, C:N ratios decreased along with a decrease in fibre level. Under controlled conditions, nitrogen represents a major influence on the development of intensive tank cultivation of Ulva rigida, not only by affecting parameters closely related to nitrogen metabolism but also some clearly influenced by carbon uptake.
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  • 49
    ISSN: 1573-5176
    Keywords: herbicide ; DCMU ; photosynthesis ; monoalgal culture ; Chaetoceros ; Dunaliella ; Nannochloropsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The selective effect of DCMU on photosynthetic activity and growth rate was examined in several marine unicellular algae:Nannochloropsis sp. (Eustigmatohyceae),Dunaliella salina (Chlorophyceae)Isochrysis galbana (Prymnesiophyceae) andChaetoceros sp. (Bacillariophyceae). DCMU at 10−7 M caused an immediate decrease in the photosynthetic rate ofDunaliella andIsochrysis (about 50% inhibition), whereas 10−6 M imposed 80% inhibition in the photosynthetic rate ofChaetoceros. InNannochloropsis the rate was affected only when DCMU concentration exceeded 10−6M. Cellular growth rate of all studied algae was affected by DCMU in a similar manner to photosynthesis. The differential effect of DCMU was further examined in mixed cultures in whichNannochloropsis was cultivated together with an additional species simulating a contamination situation of aNannochloropsis culture. When DCMU was added at concentrations higher than 10−7 M, the growth of the competing algae significantly decreased, whileNannochloropsis maintained a relatively high growth rate. Consequently, after a growth period of 4 to 7 days a clear domination ofNannochloropsis was observed. These results demonstrate that DCMU and probably other herbicides of similar characteristics can be used effectively as a selective tool to suppress contaminating unicellular algae in open ponds in order to maintain a monoculture ofNannochloropsis.
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  • 50
    ISSN: 1573-5176
    Keywords: Spirulina platensis ; cyanobacterium ; NaCl ; photoinhibition ; photosynthesis ; photosystem II ; salt stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response ofSpirulina platensis cells to salinity stress was studied. Once adapted to the higher osmoticum, photosynthetic parameters such as the maximum rate of photosynthesis under saturating irradiance (Pmax) and the initial slope of the P-I curve (α) are reduced by 15% and 25% in 0.5 M NaCl grown cells, respectively. Salt-adapted cells have a modified biochemical composition; reduced protein and chlorophyll content, and an increased level of carbohydrates. The reduction in the photosynthetic capacity of the salt-adaptedSpirulina cells reflects a lower ability to utilize light energy and results in an increase in the susceptibility of the stressed cells to photoinhibition. This conclusion is supported by the finding that cultures exposed to salt stress show not only a decrease in growth rate (μ), but lose the ability to respond to increased irradiance with an increase in growth. The use of variable fluorescence as a fast and reliable measurement to follow the changes in PSII of salt-stressesSpirulina cells enables following the early events of salinity shock. It indicates that as soon as the cells are exposed to salt, a protection mechanism is induced. This mechanism does not require any protein synthesis and may take place even in the dark, though at somewhat reduced effectiveness. The significance of the result in providing a better understanding of the interaction between two environmental stresses — light and salinity — and their application in the outdoor mass cultivation ofSpirulina are discussed.
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  • 51
    ISSN: 1573-5176
    Keywords: algal growth ; Porphyra ; inorganic carbon (Ci) ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthetic (oxygen evolution) and growth (biomass increase) responses to ambient pH and inorganic carbon (Ci) supply were determined for Porphyralinearis grown in 0.5 L glass cylinders in the laboratory, or in 40 L fibreglass outdoor tanks with running seawater. While net photosynthetic rates were uniform at pH 6.0–8.0, dropping only at pH 8.7, growth rates were significantly affected by pH levels other than that of seawater (c. pH 8.3). In glass cylinders, weekly growth rates averaged 76% at external pH 8.0, 13% at pH 8.7 and 26% at pH 7.0. Photosynthetic O2 evolution on a daily basis(i.e. total O2 evolved during day time less total O2 consumed during night time) was similar to the growth responses at all experimental pH levels, apparently due to high dark respiration rates measured at acidic pH. Weekly growth rates averaged 53% in algae grown in fibreglass tanks aerated with regular air (360 mg L-1 CO2) and 28% in algae grown in tanks aerated with CO2-enriched air (750 mg L-1 CO2). The pH of the seawater medium in which P. linear is was grown increased slightly during the day and only rarely reached 9.0. The pH at the boundary layer of algae submerged in seawater increased in response to light reaching, about pH 8.9 within minutes, or remained unchanged for algae submerged in a CO2-free artificial sea water medium. Photosynthesis of P. linearissaturated at Ci concentrations of seawater (K0.5560 μM at pH 8.2) and showed low photosynthetic affinity for CO2(K0.5 61 μM) at pH 6.0. It is therefore concluded that P. linearisuses primarily CO2 with HCO3 - being an alternative source of Ci for photosynthesis. Its fast growth could be related to the enzyme carbonic anhydrase whose activity was detected intra- and extracellularly.
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  • 52
    ISSN: 1573-5176
    Keywords: CO2 ; inorganic carbon ; macroalgae ; photosynthesis ; PAM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthesis and cell composition of Porphyraleucosticta discs grown at low (〈 0.0001% in air), current (control) and high (1% CO2 in air)inorganic carbon (Ci) concentrations were analyzed. Carbohydrate content in discs grown at high Ci increased (15.1 mg g-1 FW) with respect to the control (6.4 mg g FW-1), whereas soluble protein content decreased to one-third (5.6 to2.1 mg g-1 FW). Carbohydrate content was unaffected and soluble protein slightly increased in discs grown at low Ci. As a consequence of these changes, a lower C/N molar ratio (8.6) was found in the discs grown at low compared to high Ci(12.4). Nitrate reductase activity increased at high Ci from 0.3 ± 0.2 to 1.7 ± 0.4 μmolNO2 - g-1 FW h-1indicating that reduction and assimilation of nitrate were uncoupled. The response of photosynthesis to increasing irradiance, estimated from O2evolution vs. irradiance curves, was affected by the treatments. Maximum quantum yield (Φ O2°) and effective quantum yield (Φ O2) at 150 μmol photon m-2s-1 decreased by 20% and 50%, respectively, at low Ci. These differences could be due to changes in photosynthetic electron flow between PSII and PSI. Treatments also produced changes in maximal (Fv/Fm) and effective (ΔF/Fm′)quantum yield for photosystem II charge separation.
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  • 53
    ISSN: 1573-5176
    Keywords: cell-wall polysaccharides ; chlorophylla ; Gelidium sesquipedale ; internal C and N ; light quality ; photosynthesis ; phycobiliproteins ; respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of different light qualities on the photosynthetic rate, dark respiration, intracellular carbon and nitrogen content, and accumulation of photosynthetic pigments and cell-wall polysaccharides during short-term incubation (5 h) of the red algaGelidium sesquipedale was investigated. The same photon irradiance of 50μmol m−2 s−2 below the light saturation point of photosynthesis was applied in each case. Blue light stimulated photosynthesis, dark respiration and the accumulation of chlorophyll and biliproteins, phycoerythrin in particular. The accumulation of internal carbon and nitrogen was greater under blue light than under the other light qualities. In contrast, the percentage of cell-wall polysaccharides was higher in red light. The content of cell-wall polysaccharides decreased during the time of incubation in all light treatments except in red light. The action of a non-photosynthetic photoreceptor in the control of cell-wall polysaccharide synthesis is suggested because the accumulation of cell-wall polysaccharides was not correlated with net photosynthesis in contrast to what occurred with carbon, chlorophyll and phycoerythrin accumulation.
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  • 54
    ISSN: 1573-5176
    Keywords: cyanobacteria ; light intensity ; photoinhibition ; photosynthesis ; recovery ; Spirulina platensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three isolates ofSpirulina platensis (Norst) Geitler marked BP, P4P and Z19/2 were compared with respect to their response and acclimation capability to high photon flux densities (HPFD). Cultures exposed to HPFD (1500–3500 μmol photon m−2 s−1) exhibited a marked decrease in light-dependent O2 evolution rate. P4P was more sensitive to HPFD than the two other isolates. All three isolates recovered from photoinhibition when placed under low PFD. The BP isolate was able to recover also in the dark but to a lower extent and at a lower rate, while no recovery was observed in the other two isolates under dark conditions. No recovery was observed when protein synthesis was inhibited using chloramphenicol. Cultures grown at 200 μmol photon m−2 s−1 differed from cultures grown at 120 μmol photon m 2 s-1 by their lower maximal photosynthetic rate (P max ) and higher light saturation (I k ) value, while being more resistant to HPFD stress. The ability ofSpirulina isolates to acclimate and withstand HPFD may provide useful information for the selection of strains useful for outdoor mass cultivation.
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  • 55
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    Journal of applied phycology 9 (1997), S. 525-532 
    ISSN: 1573-5176
    Keywords: Gelidiella ; agarophyte ; photosynthesis ; P-I curve ; morphology ; tide ; pigment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The red seaweed agarophyte, Gelidiella acerosa (Forsskål) (Feldmann& Hamel) was collected from tidepools, high intertidal rocks. and shallow subtidal are as along a reef flat in Ilocos Norte, northern Philippines. The three populations were compared during the summer (dry) and rainy (wet) seasons to determine changes in morphology and photoacclimation capacity as possible use in mariculture. During summer months (February toApril) after exposure to environmental extremes (i.e. the highest percent of minus tides during daylight, high light regimes, desiccation, and solar bleaching), the populations differed in their morphologies and responses to increasing irradiance levels (P–I curve). Tidepool plants were the tallest, bushiest, and with increased diameter of cortical cells; while,high intertidal plants were the shortest, with sparse branching pattern and decreased diameter of cortical cells. Although their saturation irradiances indicated shade tolerance (Ik = 52 − 112 µmol photon m -2 s-1). their differential light saturation curves (P-I curves) suggested a capacity to acclimate to ambient light regimes. For example, plants from the high intertidal zone showed higher photosynthetic rates and saturation irradiances, slightly lower initial slopes of the P-I curves and levels of light harvesting accessory pigments, rphycoerhythrin (R-PE) and rphycocyanin (R-PC), after being exposed to higher light regimes. In contrast, plants from tidepools and shallow subtidal areas had lower photosynthetic rates and saturation irradiances, slightly steeper initial slopes of the P-I curves and levels of R-PE and R-PC, having been exposed to lower light regimes. During the rainy months (June to November) no significant responses in these parameters were recorded. Comparison of the P-I responses of vegetative and tetrasporic plants showed these to vary with season. The data suggest that when plants became reproductive their physiological fitness either was unchanged or slightly enhanced. These results indicate that all three populations of G. acerosa could be used as seed stock for mariculture.
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  • 56
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    Journal of applied phycology 9 (1997), S. 503-510 
    ISSN: 1573-5176
    Keywords: Chlorella pyrenoidosa ; dense algalsuspension ; light-harvesting pigment ; photosynthesis ; Synechocystis PCC 6714
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of light-harvesting pigments (LHP) inmicroalgal cells on photosynthetic activity in adense cell suspension were examined. The results suggest that a lower LHP content should result in higher photosynthetic productivity under high light intensity. The idea was first proposed by Lien and San Pietro in 1975 that photosynthesis could be improved by reducing the LHP content in microalgal cells, but this has not been demonstrated in detail. Experiments to evaluate the idea were conducted with Synechocystis PCC6714 and Chlorellapyrenoidosa. In the experiments with PCC 6714, photosynthesis of a phycocyanin-deficient mutant was compared with that of the wild type. In the experiments with C. pyrenoidosa, the LHP content was controlled by the light intensity in the algalculture. The maximum photosynthetic activity was 20–30% higher in the dense suspension of cells having a lower LHP content with both organisms. These results indicate that the idea of reducing the LHP contentcould be applicable to a wide variety of photosynthetic organisms.
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  • 57
    ISSN: 1573-5176
    Keywords: Gelidium sesquipedale ; photosynthesis ; fluorescence ; light response curves ; pigments ; depth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthesis-light response curves of Gelidium sesquipedale from the west coast of Portugal (Cape Espichel) were determined at four different depths, 3, 10, 15 and 22 m. Data acquisition using chlorophyll a fluorescence methodology and oxygen electrode measurements were compared. Response curves were determined over an increasing range of irradiance values (I), from darkness to 900 μmol photon m-2 s-1 PAR. In general, light response curves obtained for G. sesquipedale showed a similar pattern whether determined by the chlorophyll fluorescence method or by oxygen evolution. The photosynthetic capacity of G. sesquipedale decreased with depth, as expected, revealing a ‘sun’ and ‘shade’ acclimation pattern, between shallow and deeper waters.
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  • 58
    ISSN: 1573-5176
    Keywords: UV-radiation ; chlorophyll fluorescence ; photosynthesis ; stress tolerance ; electron transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photoinhibition and recovery kinetics after short exposure to solar radiation following three different irradiance treatments of irradiances (PAR, PAR+UVA and PAR+UVA+UVB) was assessed in two intertidal species of the genus Gelidium, Gelidium sesquipedale and G. latifolium, collected from Tarifa (southern Spain) using in vivo chlorophyll fluorescence (PAM fluorometry). After 3 h UV radiation exposure, optimal quantum efficiency (Fv/Fm) in G. sesquipedale decreased between 25 and 35% relative to the control. Under PAR alone, values decreased to 60%. In G. latifolium, photoinhibition did not exceed 40%. Similar results were found for the effective quantum yield (ΔF/Fm′), however, no marked differences in relation to light treatments were seen. When plants were shaded for recovery from stress, only in G. latifolium a significant increase in photosynthesis was observed (between 80 and 100% of control). In contrast, photosynthesis of G. sesquipedale suffered a chronic photoinhibition or photodamage under the three light irradiances. Full solar radiation (PAR+UVA+UVB) affected also the electron transport rate in both species. Here, initial slopes of electron transport vs. irradiance curves decreased up to 60% of controls. Although the recovery kinetic under PAR+UVA+UVB conditions was delayed in G. latifolium, after 24 h recovery this species reached significantly higher than G. sesquipedale. PAR impaired electron trasport only in G. sesquipedale. Overall, both species are characterized by different capacity to tolerate enhanced solar radiation. G. latifolium is a sun adapted plant, well suited to intertidal light conditions, whereas G. sesquipedale, growing at shaded sites in the intertidal zone, is more vulnerable to enhanced UV radiation.
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  • 59
    ISSN: 1573-5176
    Keywords: 14C ; photosynthesis ; population growth ; Selenastrum capricornutum ; suspended sediment elutriate ; zinc ; cadmium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Short-term 14C-fixation (4 h) Selenastrum capricornutum algal toxicity tests were conducted with Cd (n=8), Zn (n=9) and suspended sediment aqueous elutriates (n=28) and the results were compared to those obtained in a 48 h population growth test. In order to provide more realistic experimental conditions, toxicity tests were carried out in prefiltered nutrient-spiked Lake Geneva water. The population growth inhibition test was significantly more sensitive than the14 C-fixation test for Cd (median EC50-4h and EC50-48h values of 600 and 118 µg L-1, respectively) whereas no significant difference was measured for Zn toxicity (median EC50-4h and EC50-48h values of 97 and 96 µg L-1, respectively). With suspended sediment aqueous elutriates, the relative sensitivity of the two different end points is sample dependent, with ratios of the EC25 for the14 C-fixation: population growth test ranging from 〈0.26 to 〉53.3. Elutriate toxicity shows no apparent relationship between the acute and chronic test, indicating that population growth inhibition cannot be derived directly or predicted from14 C-fixation. Both tests with their specific advantages and limitations provide valuable complementary information to measure the impact of single toxicants or complex mixtures on aquatic plants.
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  • 60
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    Journal of applied phycology 10 (1998), S. 447-452 
    ISSN: 1573-5176
    Keywords: dense algal suspension ; light-harvesting pigment ; photosynthesis ; Synechocystis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects on photoinhibition of light-harvesting pigments in microalgal cells were examined using the wild type and a phycocyanin- deficient mutant (PD-1) of Synechosystis PCC 6714. Mutant PD-1 showed higher resistance to high light than the wild type in terms of the decline of photosynthetic activity at any light intensity and with various cell densities. This suggests that the loss of productivity induced by high light intensity would be improved by reducing the content of light-harvesting pigments.
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  • 61
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    Journal of applied phycology 11 (1999), S. 473-477 
    ISSN: 1573-5176
    Keywords: aquaculture ; light ; photosynthesis ; Porphyra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Due to their rapid growth and nutrient assimilation,Porphyra spp. are good candidates for bioremediation and polyculture. The production potential of two strains of P. purpurea and P. umbilicalis from north-east USA was evaluated by measuring rates of photosynthesis (as O2evolution) of material grown at 20 °C. Photosynthetic rates of P. umbilicalis were 80%higher than P. purpurea over the temperature range 5–20 °C, at both sub-saturating andsaturating irradiances (37 and 289 μmol photonm-2 s-1). Porphyra umbilicalis was more efficient at low irradiances (higher α) and had a higher Pmax (23.0 vs 15.6 μmolO2 g-1 DW min-1) than P.purpurea, suggesting that P. umbilicalis is a better choice for mass culture, where self-shading maybe severe.
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  • 62
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    Journal of applied phycology 11 (1999), S. 535-541 
    ISSN: 1573-5176
    Keywords: cyanobacterium ; Nostoc flagelliforme ; nutrients ; photosynthesis ; potassium ; re-hydration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of nutrients on the photosynthetic recovery of Nostoc flagelliforme during re-hydration were investigated in order to see if their addition was necessary. Net photosynthesis was negligible in distilled water without nutrient-enrichment. Addition of K+ resulted in significant enhancement of net photosynthesis, whereas other nutrients (Fe3+, Mg2+, Na+, NO3 -, PO4 3-, Cl-) and trace-metals (A5) showed little effect. The recovered net photosynthetic activity increased with the increased K+, and reached the maximum at concentrations above 230 μM. Desiccation and re-hydration did not affect the dependence of photosynthetic recovery on K+. It was concluded that dried field populations of N. flagelliforme require exogenous addition of potassium for photosynthetic recovery and that growth may be potassium-limited in nature.
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  • 63
    ISSN: 1573-5125
    Keywords: sherbicide tolerance ; photosynthesis ; phytoplankton ; simetryn ; triazine ; herbicides ; ultraviolet radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the effects of UV B radiation on14C-uptake rates and carbon assimilation into the major end-products of photosynthesis of the green algaScenedesmus in the presence and absence of the triazine herbicide simetryn. Experiments were conducted using both a herbicide-susceptible and herbicide-tolerant strains ofScenedesmus. Three different UV-B dose rates were used as well as a light control. The lowest dose rate was almost the same level as in subsurface of ponds and lakes, while the other two were slightly lower and higher than natural sunlight on the surface of ponds and lakes, respectively. Total uptake rates of14C were not reduced by the UV B irradiation alone even at the highest dose rate. However, in the presence of the herbicide, uptake rates were clearly reduced by the highest dose rate of UV-B concomitant with increasing herbicide concentrations in the herbicide-susceptible strain. On the other hand, the proportion of lipid fraction was slightly reduced by all the UV-B treatments in the herbicide-susceptible strain even in the absence of the herbicide. In the herbicide-tolerant strain, uptake rates were not affected by UV-B radiation or by the herbicide. These facts indicated that UV-B effects could be smaller than predicted. It may be important to examine combined effects of UV-B and other anthropogenic and/or natural stresses for assessing actual UV-B effects in the field.
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  • 64
    ISSN: 1573-5125
    Keywords: cryptomonads ; macromolecular ; Phototron ; photosynthesis ; UV radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We used a device called a Phototron to measure the effects of UV radiation on the cosmopolitan algae, Cryptomonas erosa, grown in continuous cultures. In the Phototron, we investigated changes in photosynthetic parameters (Pmax – specific production rate at optimal light intensity; α – initial slope of the linear portion of the Photosynthesis-Irradiance curve; and θ – the convexity or rate of bending) and carbon allocation as a function of irradiance at three different environmentally-realistic doses of UV radiation in unconditioned (no prior UV exposure) and conditioned algae (15 d previous UV exposure). For unconditioned control algae, Pmax-Total was lower, although not significantly, than the two highest UV treatments. For conditioned control algae, Pmax-Total was higher, although not significantly, than all UV treatments. Our data suggest that short term (4 h) exposure to low levels of UV (8.09 W m−2 unweighted) does not affect Pmax-Total in C. erosa, but does change the proportion of carbon allocated to lipids and proteins. Also, comparisons of lipids, polysaccharides and proteins as a percent of total carbon uptake between unconditioned and conditioned algae indicate that exposure history to UV radiation can have a negative impact on carbon allocation to lipids and proteins, in a wetland alga species that is crucial to the efficient transfer of energy through freshwater food webs.
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  • 65
    ISSN: 1573-868X
    Keywords: Carbon dioxide ; coral reefs ; atoll ; lagoon ; photosynthesis ; calcification ; total alkalinity ; partial pressure ; topography ; residence time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Factors controlling the CO2 system parameters, including the partial pressure of CO2 (PCO2) in coral reef waters, were investigated in three mid-oceanic reefs of the Indo-Pacific region. Surface water PCO2 in the lagoons of Majuro Atoll and Palau barrier reef in the Pacific were 25 µatm and 48 µatm higher than those of the offshore waters, respectively, while South Male Atoll lagoon of the Maldives in the Indian Ocean exhibited relatively small difference in PCO2 compared to the offshore water. Observations from Majuro Atoll and Palau barrier reef are consistent with the view that calcium carbonate production predominates in coral reefs. On the other hand, results from South Male Atoll can be attributed to the thorough flushing of the lagoon, which is connected to the open ocean by numerous deep channels. The offshore-lagoon PCO2 difference depends on system-level net organic-to-inorganic carbon production ratio while reef topography, especially residence time of the lagoon, has a secondary effect on the magnitude of the offshore-lagoon difference. A potential for releasing CO2 might be more evident in an enclosed atoll where the reef water has a longer residence time. Oceanic atoll and barrier reef lagoons, which are in the terminal stage of evolutionary history of oceanic volcanic islands, have the potential to release CO2 to the atmosphere.
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  • 66
    ISSN: 1573-5117
    Keywords: gas exchange ; mangrove ; photosynthesis ; salinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Continuous measurements of gas exchange characteristics were made on two to nine year old hydroponically grown Avicennia germinans (L.) Stearn, Aegialitis annulata R. Br. and Aegiceras corniculatum (L.) Blanco maintained at 50 or 500 mol m−3 NaCl. In Avicennia germinans and Aegialitis annulata, CO2 assimilation rates were initially higher at 500 mol m−3 NaCl and decreased gradually towards the end of the photoperiod when rates were similar to those at the lower salinity. In Aegiceras corniculatum, assimilation rates were higher at 50 mol m−3 NaCl and about 55% lower at the higher salinity. In all three species, leaf conductance and transpiration exhibited trends similar to those for CO2 assimilation. Intercellular CO2 concentrations were similar at both salinities in Avicennia germinans and Aegialitis annulata, but considerably higher at the lower salinity in Aegiceras corniculatum. Water use efficiencies (WUE), although similar between salinity treatments in Avicennia germinans and Aegialitis annulata, were greater at the higher salinity in Aegiceras corniculatum. Data obtained from CO2 response curves indicated that assimilation at high salinity in Aegiceras corniculatum was limited by conductance, and to a lesser extent, by photosynthetic capacity. In Avicennia germinans and Aegialitis annulata, assimilation was greater at the higher salinity as indicated by increase in both the initial slope and the upper plateau of the CO2 response data. Greater assimilation at high salinity in Avicennia germinans and Aegialitis annulata may be attributed to lower carbon losses via photorespiration and to efficient salt excretion and sequestration.
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  • 67
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    Hydrobiologia 364 (1997), S. 199-208 
    ISSN: 1573-5117
    Keywords: Phytoplankton ; photosynthesis ; light ; temperature ; tidal freshwater ; irradiance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photosynthetic response to irradiance wasquantified for phytoplankton from the tidalfreshwater Potomac River biweekly to monthly over aperiod of six years. Samples were collected from twoshallow embayments and portions of the deeper rivermainstem. Photosynthetic rate was measured in thelaboratory at in situ temperature over a range ofirradiance levels and photosynthetic parameters werecalculated using nonlinear regression.PB max,the maximum photosynthetic ratestandardized to chlorophyll a, increased withtemperature up to 25 °C with a Q10 of 2.02. Above 25 °C, PB max was essentiallyconstant with temperature. Lesser correlationbetween PB max and ambient irradiance couldbe explained by the correlation of irradiance withtemperature. α, the slope of the P–I curve atlow light, was correlated with both ambientirradiance and temperature. Highest α valueswere found in late summer when high temperature andintermediate ambient irradiance were observed. Spring and early summer were characterized by lowα. Despite low light penetration, Ik andα values were indicative of sun limitationpossibly due to intermittent high light levelsexperienced during mixing. Ik showed a clearseasonal trend directly related to days from summersolstice. Spatial patterns were minimal except thatIk was consistently lower in one shallowembayment than in the other two areas. Seasonalpatterns in photosynthetic parameters correspondedroughly to changes from a spring diatom populationto summer cyanobacterial assemblage.
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  • 68
    ISSN: 1573-5117
    Keywords: photon flux density ; intracellular metabolic pools ; proteins ; carbohydrates ; lipids ; polysaccharides ; photosynthesis ; phytoplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of photon flux density (PFD) on the partitioning of photosynthetically fixed 14CO2-C into major intracellular end products was investigated for three species of freshwater planktonic algae (Nitzschia palea, Monoraphidium minutum and Synechococcus elongatus belonging to three different classes. This study was designed to investigate the phenomenon of polysaccharide synthesis associated with the saturation of protein synthesis and to test if this process is common to all three phytoplankton species. Protein synthesis was saturated at low PFD in all three species of algae studied. However, fixed carbon was differentially stored, namely in lipids in Nitzschia palea (Bacillariophyceae), in polysaccharides in Monoraphidium minutum (Chlorophyceae), and in low molecular weight metabolites (LMW) in Synechococcus elongatus (Cyanophyceae). The results of this transient state study indicate that the metabolic pathways of algae can easily be controlled by different irradiance. Furthermore, it appears that the difference in the patterns of synthesis is taxonomy dependent.
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  • 69
    ISSN: 1573-5117
    Keywords: biotest ; brackish ; Gracilaria ; growth inhibition ; marine ; photosynthesis ; Rhodophyta ; seaweed ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A growth inhibition test method was developed using the macroalga Gracilaria tenuistipitata as the test organism. This alga was chosen because of its high laboratory growth rates, commonly 30–40% d−1, which are reached in salinities between 5 and 40‰, and its epiphyte resistance. The toxicity of a number of substances, including heavy metals, herbicides and complex wastewaters towards the alga was assayed. Anti-fouling paints were tested with a modification of the method. EC50 values for heavy metals varied between 0.05 and 17 mg l−1 and for herbicides between 0.002 and 0.02 mg l−l. The sensitivity to the toxicant was generally higher at low salinity. Omitting nitrogen and phosphorus additions to the test medium increased the sensitivity and a semi-static performance was possible with maintained or increased sensitivity. Preliminary tests done with a computerised photosynthesis inhibition method produced promising results. In conclusion, this is a simple, sensitive and reproducible test method for assessing the toxicity of substances, wastewaters and anti-fouling paints in brackish and marine environments.
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  • 70
    ISSN: 1573-5117
    Keywords: Avicennia marina ; gas exchange ; mangroves ; photosynthesis ; waterlogging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study was undertaken in summer on fully expanded leaves of Avicennia marina trees in the Beachwood Mangroves Nature Reserve, Durban,South Africa. Data sets were obtained over 5–7 days of relatively dry conditions and over two periods of 5 days during which the swamp was continuously inundated with dilute seawater (〈 150 mol m−3NaCl). Gas exchange responses were strongly influenced by photosynthetic photon flux density (PPFD), leaf temperature and leaf to air vapour pressure deficit (Δw). Carbon dioxide exchange was saturated at a PPFD of about 800 µmol m−2 s−1. Maximal CO2 exchange rates ranged from 8.5 to 9.9 µmol m−2 s−1 with no differences between drained and waterlogged conditions. Under drained conditions, leaf conductance,transpiration and internal CO2 concentrations were generally lower, and water use efficiencies higher, than during waterlogging. Continuous waterlogging for 5 days had no adverse effect on CO2 exchange. Xylem water potentials ranged from −1.32to −3.53 MPa during drained and from −1.02 to −2.65 Mpa during waterlogged conditions. These results are discussed in relation to anatomical and metabolic adaptations of A. marina to waterlogging stress.
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  • 71
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    Bioscience reports 19 (1999), S. 499-509 
    ISSN: 1573-4935
    Keywords: Arabidopsis ; auxiliary enzymes ; light stress ; photosynthesis ; protein phosphorylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An improved cultivation system for Arabidopsis thaliana was developed, allowing advanced biochemical studies in vitro and in vivo of this important model plant. Highly functional Arabidopsis thylakoids were isolated and used to study both basic and regulatory photosynthetic functions with the aim to create a platform for the characterization of mutants deficient in auxiliary proteins. Light-induced proteolytic degradation of the D1 protein could be followed and shown to be a subsequent event to photoinactivation of electron transport. The phosphorylation and dephosphorylation of thylakoid proteins resembled that seen in spinach leaves although phospho-CP43 revealed an unusual regulatory behavior.
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  • 72
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    Plant molecular biology 35 (1997), S. 407-416 
    ISSN: 1573-5028
    Keywords: cyanobacteria ; cytochrome oxidase ; electron transport ; photosynthesis ; respiration ; thylakoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyanobacterial thylakoids catalyze both photosynthetic and respiratory activities. In a photosystem I-less Synechocystis sp. PCC 6803 strain, electrons generated by photosystem II appear to be utilized by cytochrome oxidase. To identify the lumenal electron carriers (plastocyanin and/or cytochromes c 553, c 550, and possibly c M) that are involved in transfer of photosystem II-generated electrons to the terminal oxidase, deletion constructs for genes coding for these components were introduced into a photosystem I-less Synechocystis sp. PCC 6803 strain, and electron flow out of photosystem II was monitored in resulting strains through chlorophyll fluorescence yields. Loss of cytochrome c 553 or plastocyanin, but not of cytochrome c 550, decreased the rate of electron flow out of photosystem II. Surprisingly, cytochrome c M could not be deleted in a photosystem I-less background strain, and also a double-deletion mutant lacking both plastocyanin and cytochromec 553 could not be obtained. Cytochrome c M has some homology with the cytochrome c-binding regions of the cytochromecaa3 -type cytochrome oxidase from Bacillus spp. and Thermus thermophilus. We suggest that cytochrome c M is a component of cytochrome oxidase in cyanobacteria that serves as redox intermediate between soluble electron carriers and the cytochromeaa3 complex, and that either plastocyanin or cytochrome c 553 can shuttle electrons from the cytochrome b6f complex to cytochrome c M.
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  • 73
    ISSN: 1573-5028
    Keywords: ferredoxin-NADP+ oxidoreductase ; petH ; divergent operator ; antisense mRNA ; phosphoribulokinase ; prk Synechocystis PCC 6803 ; photosynthesis ; cyanobacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The petH gene, encoding ferredoxin-NADP+ oxidoreductase (FNR), has been characterised in the unicellular cyanobacterium Synechocystis PCC 6803. Its product, FNR, was heterologously produced and functionally characterized. The start-site of the monocystronic petH transcript was mapped 523 bp upstream of the predicted PetH initiation codon, resulting in an unusually large 5′-untranslated region. The 5′ end of the petH transcript is situated within the open reading frame of phosphoribulokinase (encoded by prk), which is transcribed in opposite orientation with respect to petH. The transcription start site of the prk transcript was mapped 219 bp upstream of the initiation codon, resulting in a 223 bp antisense region between both transcripts. Under many conditions the expression of both genes (i.e. petH and prk) is co-regulated symmetrically at the transcriptional level, as was concluded from both northern hybridization experiments and from primer extension analyses; it became uncoupled, however, when specifically petH expression was stimulated, independent of prk expression, by stressing the Synechocystis cells with high salt concentrations. A model for a new type of bidirectional operator, regulating the expression of petH and prk, is proposed.
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  • 74
    ISSN: 1573-5079
    Keywords: chlorophyll fluorescence ; energy dissipation ; light scattering ; photosynthesis ; state transition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The light-induced induction of components of non-photochemical quenching of chlorophyll fluorescence which are distinguished by different rates of dark relaxation (qNf, rapidly relaxing and qNs, slowly relaxing or not relaxing at all in the presence brief saturating light pulses which interrupt darkness at low frequencies) was studied in leaves of spinach. After dark adaptation of the leaves, a fast relaxing component developed in low light only after a lag phase. Quenching increased towards a maximum with increasing photon flux density. This ‘fast’ component of quenching was identified as energy-dependent quenching qE. It required formation of an appreciable transthylakoid ΔpH and was insignificant when darkened spinach leaves received 1 s pulses of light every 30 s even though zeaxanthin was formed from violaxanthin under these conditions. Another quenching component termed qNs developed in low light without a lag phase. It was not dependent on a transthylakoid pH gradient, decayed exponentially with a long half time of relaxation and was about 20% of total quenching irrespective of light intensity. When darkened leaves were flashed at frequencies higher than 0.004 Hz with 1 s light pulses, this quenching also appeared. Its extent was very considerable, and it did not require formation of zeaxanthin. Relaxation was accelerated by far-red light, and this acceleration was abolished by NaF. We suggest that qNs is the result of a so-called state transition, in which LHC II moves after its phosphorylation from fluorescent PS II to nonfluorescent PS I. This state transition was capable of decreasing in darkened leaves the potential maximum quantum efficiency of electron flow through Photosystem II by about 20%.
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  • 75
    ISSN: 1573-5079
    Keywords: cyanobacteria ; electron transport ; fluorescence ; photosynthesis ; spillover ; state changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adaptive responses to excess (supraoptimal) level of cobalt supplied to the growth medium were studied in the cyanobacterium Synechocystis PCC 6803. Growth of cells in the medium containing 10 μM CoCl2 led to a large stimulation (50%) in O2-evolution and an overall increase (∼30%) in the photosynthetic electron transport rates. Analysis of variable Chl a fluorescence yield of PS II and immuno-detection of Photosystem II (PS II) reaction-center protein D1, showed a small increase (15–20%) in the number of PS II units in cobalt-grown cells. Cobalt-grown cells, therefore, had a slightly elevated PS II/PS I ratio compared to control. We observed alteration in the extent of energy distribution between the two photosystems in the eobalt grown cells. Energy was preferentially distributed in favour of PS II accompanied by a reduction in the extent of energy transfer from PS II to PS I in cobalt-grown cells. These cells also showed a smaller PS I absorption cross-section and a smaller size of intersystem electron pool than the control cells. Thus, our results suggest that supplementation of 10 μM CoCl2, to the normal growth medium causes multiple changes involving small increase in PS II to PS I ratio, enhanced funneling of energy to PS II and an increase in PS I electron transport, decrease PS I cross section and reduction in intersystem pool size. The cumulative effects of these alterations cause stimulation in electron transport and O2 evolution.
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  • 76
    ISSN: 1573-5087
    Keywords: carbohydrates ; elevated CO2 ; Gossypium hirsutum L. ; interaction ; photosynthesis ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cotton (Gossypium hirsutum L., cv DPL 5415) plants were grown in naturally lit environment chambers at day/night temperature regimes of 26/18 (T-26/18), 31/23 (T-31/23) and 36/28 °C (T-36/28) and CO2 concentrations of 350 (C-350), 450 (C-450) and 700 μL L-1 (C-700). Net photosynthesis rates, stomatal conductance, transpiration, RuBP carboxylase activity and the foliar contents of starch and sucrose were measured during different growth stages. Net CO2 assimilation rates increased with increasing CO2 and temperature regimes. The enhancement of photosynthesis was from 24 μmol CO2 m-2 s-1 (with C-350 and T-26/18) to 41 μmol m-2 s-1 (with C-700 and T-36/28). Stomatal conductance decreased with increasing CO2 while it increased up to T-31/23 and then declined. The interactive effects of CO2 and temperature resulted in a 30% decrease in transpiration. Although the leaves grown in elevated CO2 had high starch and sucrose concentrations, their content decreased with increasing temperature. Increasing temperature from T-26/18 to 36/28 increased RuBP carboxylase activity in the order of 121, 172 and 190 μmol mg-1 chl h-1 at C-350, C-450 and C-700 respectively. Our data suggest that leaf photosynthesis in cotton benefited more from CO_2 enrichment at warm temperatures than at low growth temperature regimes.
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  • 77
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    Photosynthesis research 51 (1997), S. 149-159 
    ISSN: 1573-5079
    Keywords: abscisic acid ; t Acacia confusa ; t Leucaenaleucocephala ; photosynthesis ; soil drying ; stomatal conductance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effects of leaf water deficit and increase in endogenous ABA on photosynthesis of two tropical trees, t Acacia confusa and t Leucaena leucocephala, were investigated with two soil-drying methods, i.e. half or whole root system was subjected to soil drying. Half-root drying was achieved by allowing upper layer of soil column to dry and lower layer of soil column to remain watered. Half-root drying had little effect on leaf water potential, but when compared to the well-watered control, both methods of soil drying substantially increased the ABA concentration in xylem and reduced leaf conductance in both species. There was a significant relationship between leaf conductance and xylem ABA concentrations in both species, which was comparable to the same relationship that was generated by feeding ABA to excised twigs. The rate of photosynthesis was inhibited substantially in both soil-drying treatments and in both species, but photochchemical efficiency, measured as a ratio of variable fluorescence to a peak fluorescence emission of a dark-adapted leaf (Fv/Fm), was not reduced except in the whole root-dried t L. leucocephala plants where leaf water potential was reduced to –2.5 MPa. In all the cases where photosynthesis was inhibited, there was a concomitant reduction in both leaf conductance and calculated internal CO2 concentration. After two days of rewatering, leaf water potential and xylem ABA concentration rapidly returned to pre-treatment levels, but leaf conductance and photosynthesis of both whole-root and half root dried t L. leucocephala remained inhibited substantially. Rewatering led to a full recovery of both stomatal conductance and photosynthesis in soil-dried t A. confusa, although its photosynthesis of whole-root dried plants did not recover fully but such difference was not significant statistically. These results suggest that drought-induced decline of photosynthesis was mainly a result of the stomatal factor caused by the increase of ABA concentration in the xylem sap. Non-stomatal factors, e.g. reduced photochemical activity and/or carbon metabolic activity, were species-specific and were brought about only at very low water potential.
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  • 78
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    Photosynthesis research 51 (1997), S. 185-192 
    ISSN: 1573-5079
    Keywords: CAB ; cytochrome f ; photosynthesis ; Rubisco ; Rubisco activase ; senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this report we examine the factors that regulate photosynthesis during leaf ontogeny in y3y3 and Y11y11, two chlorophyll-deficient mutants of soybean. Photosynthetic rates were similar during wild type and Y11y11 leaf development, but the senescence decline in photosynthesis was accelerated in y3y3. Photosynthetic rates fell more rapidly than chlorophyll concentrations during senescence in wild type leaves, indicating that light harvesting is not strongly limiting for photosynthesis during this phase of leaf development. Chlorophyll concentrations in Y11y11, though significantly lower than normal, were able to support normal photosynthetic rates throughout leaf ontogeny. Chlorophyll a/b ratios were constant during leaf development in the wild type, but in the mutants they progressively increased (y3y3) or decreased (Y11y11). In all three sets of plants, photosynthetic rates were directly proportional to Rubisco contents and activities, suggesting that Rubisco plays a dominant role in regulating photosynthesis throughout leaf ontogeny in these plants. The expression of some photosynthetic proteins, such as Rubisco activase, was coordinately regulated with that of Rubisco in all three genotypes, i.e. an early increase, coincident with leaf expansion, followed by a senescence decline in the fully-expanded leaf. On the other hand, the light harvesting chlorophyll a/b-binding proteins of PS II (the CAB proteins), while they showed a profile similar to that of Rubisco in the wild type and y3y3, progressively increased in amount during Y11y11 leaf development. We conclude that Y11y11 may be defective in the accumulation of a component required for LHC II assembly or function, while y3y3 has more global effects and may be a regulatory factor that controls the duration of senescence.
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  • 79
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    Wetlands ecology and management 4 (1996), S. 245-256 
    ISSN: 1572-9834
    Keywords: Anaerobiosis ; baldcypress ; cherrybark oak ; nuttall oak ; photosynthesis ; productivity ; soil redox potential ; stomatal conductance ; waterlogging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seedlings of baldcypress (Taxodium distichum), nuttall oak (Quercus nuttalli), and cherrybark oak (Quercus falcata var.pagodaefolia) were subjected to four flooding treatments: control, continuously flooded, intermittently flooded, and partially flooded for 70 days in a greenhouse. The treatments imposed various durations and intensities of soil redox potential (Eh) conditions representing a range encountered by plants in their habitats. Morphological changes and gas exchange responses to the treatments differed among the study species. Rapid development of adventitious root and hypertrophied lenticels were observed in baldcypress and nuttall oak under all flooded treatments. Cherrybark oak had the highest percentage reduction in net photosynthesis ranging from 65–87%, whereas reductions in nuttall oak ranged between 35–68% and in baldcypress between 6–21% in response to various treatments. Recovery of gas exchange was noted in baldcypress but no significant recovery was found in oaks. The recovery in baldcypress contributed to the continued growth and biomass accumulation under various treatments. Little evidence of consistent changes in biomass allocation patterns in response to the treatments was found in baldcypress but total biomass decreased significantly under the continuously flooded treatment. In oaks, total biomass decreased significantly in all flooded treatments. The present findings demonstrated that physiological functions are adversely affected by low soil Eh conditions and the extent of such effects are dependent on the intensity and duration of soil reduction as well as the species' capability to respond to such conditions rapidly. Management plans concerned with regeneration of bottomland forested ecosystems should consider the species flood response capabilities at seedling stages as well as the timing, durations, and intensities of soil reduction at the specific site.
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  • 80
    ISSN: 1573-5036
    Keywords: nitrogen ; nutrient availability ; photosynthesis ; Scots pine ; seasonal change ; site quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The relationship between light saturated net photosynthesis (Amax) and nitrogen concentration (N) was studied in needles of both Scots pine seedlings, grown at three relative growth rates (2,6 and 8%) controlled by nutrient addition rate, and Scots pine shoots collected from four sites with different fertility. In the seedlings, Amax was measured on 14 different dates starting at the beginning of the second growing season and ending when growth of the new shoot and the secondary needles had finished. In shoots from the natural stands Amax of the previous-year shoots was measured on 6 dates throughout the growing season. Both in seedlings and shoots, the correlation between Amax and N was poor, when data from all sampling dates were taken together. However, Amax was correlated with N in most instances when the age of the needles was considered and the data were examined either at weekly intervals (seedlings) or separately for each sampling date (shoots). The slope of the Amax vs N relationship varied greatly between sampling dates. In the seedlings the correlation between Amax and N was strongest by the time when the new needles were developing. In the shoots the correlation was significant from mid June until mid August, while no correlation was found in the beginning and at the end of the growing season. Our data indicate that in pine needles the photosynthesis-nitrogen relationship is more complex than in broadleaved species. Contrary to the broadleaved species, where the correlation is independent of sampling time, in this conifer the time of the year affects the correlation and there are phases during the growing season when the correlation is poor or nonexistent.
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  • 81
    ISSN: 1573-5036
    Keywords: carbon budget ; growth analysis ; interspecific variation ; nitrogen supply ; photosynthesis ; respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In previous experiments systematic differences have been found in the morphology, carbon economy and chemical composition of seedlings of inherently fast- and slow-growing plant species, grown at a non-limiting nutrient supply. In the present experiment it was investigated whether these differences persist when plants are grown at suboptimal nutrient supply rates. To this end, plants of the inherently fast-growing Holcus lanatus L. and the inherently slow-growing Deschampsia flexuosa (L.) Trin. were grown in sand at two levels of nitrate supply. Growth, photosynthesis, respiration and carbon and nitrogen content were studied over a period of 4 to 7 weeks. At low N-supply, the potentially fast-growing species still grew faster than the potentially slow-growing one. Similarly, differences in leaf area ratio (leaf area:total dry weight), specific leaf area (leaf area:leaf dry weight) and leaf weight ratio (leaf dry weight:total dry weight), as observed at high N-supply persisted at low N-availability. The only growth parameter for which a substantial Species × N-supply interaction was found was the net assimilation rate (increase in dry weight per unit leaf area and time). Rates of photosynthesis, shoot respiration and root respiration, expressed per unit leaf, shoot and root weight, respectively, were lower for the plants at low N-availability and higher for the fast-growing species. Species-specific variation in the daily carbon budget was mainly due to variation in carbon fixation. Lower values at low N were largely determined by both a lower C-gain of the leaves and a higher proportion of the daily gain spent in root respiration. Interspecific variation in C-content and dry weight:fresh weight ratio were similar at low and high N-supply. Total plant organic N decreased with decreasing N-supply, without differences between species. It is concluded that most of the parameters related to growth, C-economy and chemical composition differ between species and/or are affected by N-supply, but that differences between the two species at high N-availability persist at low N-supply.
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  • 82
    ISSN: 1573-5036
    Keywords: elevated CO2 ; leaf transpiration ; nonstructural carbohydrate ; P nutrition ; photosynthesis ; white clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The growth response of white clover (Trifolium repens L.) to the expected increase in atmospheric partial pressure of CO2 (pCO2) may depend on P availability. A decrease in the rate of transpiration due to increased pCO2 may reduce the amount of P transported to the shoot, thereby causing a change in the partitioning of P between the root and shoot. To test these hypotheses, four concentrations of P in the nutrient solution, combined with two pCO2 treatments, were applied to nodulated white clover plants. Compared to ambient pCO2 (35 Pa), twice ambient pCO2 (70 Pa) reduced the rate of transpiration but did not impair the total P uptake per plant. However, at twice ambient pCO2 and a moderate to high supply of P, concentrations of structural P and soluble P (Pi) were lower in the leaves and higher in the roots. The activity of root acid phosphatase was lower at twice ambient pCO2 than at ambient pCO2; it depended on the Pi concentration in the roots. At the highest P concentration, twice ambient pCO2 stimulated photosynthesis and the growth rate of the plant without affecting the concentration of nonstructural carbohydrates in the leaves. However, at the lower P concentrations, plants at twice ambient pCO2 lost their stimulation of photosynthesis in the afternoon, they accumulated nonstructural carbohydrates in the leaves and their growth rate was not stimulated; indicating C-sink limitation of growth. P nutrition will be crucial to the growth of white clover under the expected future conditions of increased pCO2.
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  • 83
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    Plant and soil 209 (1999), S. 283-295 
    ISSN: 1573-5036
    Keywords: leaf emergence ; phosphorus ; photosynthesis ; tillering ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Phosphorus (P) deficiency limits the yield of wheat, particularly by reducing the number of ears per unit of area because of a poor tiller emergence. The objectives of this work were to (i) determine whether tiller emergence under low phosphorus availability is a function of the availability of assimilates for growth or a direct result of low P availability, (ii) attempt to establish a quantitative relation between an index of the availability of P in the plant and the effects of P deficiency on tiller emergence, and (iii) to provide a better understanding of the mechanisms involved in tiller emergence in field-grown wheat. Wheat (Triticum aestivum L., cv. INTA Oasis), was grown in the field under drip irrigation on a typic Argiudol, low in P (5.5 μg P g-1 soil Bray & Kurtz I) in Balcarce, Argentina. Treatments consisted of the combination of three levels of P fertilization 0, 60 and 200 kg P2O5 ha-1, and two levels of assimilate availability, a control (non-shaded) and 65% of reduction in incident irradiance from seedling emergence until the end of tillering (shaded). Phosphorus treatments significantly modified the pattern of growth and development of the plants. Shading reduced the growth and concentration of water-soluble carbohydrates in leaves and stems. Leaf photosynthetic rate at saturating irradiance was reduced by P deficiency, but was not affected by shading. At shoot P concentrations less than 4.2 g P kg-1 the heterogeneity in the plant population increased with respect to the number of plants bearing a certain tiller. At a shoot P concentration of 1.7 g P kg-1 tillering ceased completely. Phosphorus deficiency directly altered the normal pattern of tiller emergence by slowing the emergence of leaves on the main stem (i.e. increasing the phyllochron), and by reducing the maximum rate of tiller emergence for each tiller.
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  • 84
    ISSN: 1573-5060
    Keywords: barley landraces ; drought ; Hordeum vulgare ; leaf colour ; photosynthesis ; photosystem I and II ; thylakoid chlorophyll-proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Barley breeders at ICARDA have observed that genotypes adapted to dry regions have leaves which are lighter in colour than those of unadapted ones. We measured photosynthesis, chlorophyll content and chlorophyll a:b ratios in two sets of genotypes which had previously been observed to have either light green or dark green leaves when grown in the field. Thylakoid membranes were also extracted and the proteins analysed on SDS-PAGE gels. The light leaf colour was associated with a higher chlorophyll a:b ratio. This was a measure of a reduction in the amount of antenna chlorophyll compared to that in the core complex of PSII. Genotypes with light green leaves had consistently less chlorophyll per unit leaf area and lower photosynthetic rates per unit area than those with dark green leaves. It is suggested that these features of light green leaves may confer the ability to adapt to high levels of irradiance under drought conditions. This ability may result from a high rate of photosynthetic electron transport through each PSII reaction centre, thus reducing the risk of damage from the overexcitation of these centres.
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  • 85
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    Photosynthesis research 45 (1995), S. 157-168 
    ISSN: 1573-5079
    Keywords: chloroplast movement ; photosynthesis ; photothermal deflection spectroscopy ; Vallisneria americana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a study of photosynthetic energy storage efficiency (ES), the adaxial surface of the leaves of Vallisneria americana exhibited the highest ES values (22%) of the four aquatic plants examined. V. americana leaves have a dispersed structure and it was possible to measure the energy storage properties of the epidermal cells independently of the rest of the leaf. The abaxial epidermis had a higher value of ES at zero light fluence than the adaxial epidermis but ES in the abaxial epidermis declined much more rapidly with light fluence. Thus the abaxial epidermis is more suited to lower light fluences than the adaxial epidermis. ES declined as the pH rose from 4.0 to 8.0 at a constant dissolved inorganic carbon concentration. This paralleled the change from carbon dioxide to bicarbonate and suggests that these leaves utilise CO2 more efficiently than bicarbonate. ES increased by about 50% at pH 8.0 as leaf sections further from the leaf tip were examined which demonstrates that the older epidermal cells are less well able to use bicarbonate. Exposure to 30 min of a saturating light fluence caused the epidermal chloroplasts to move from the periclinal walls to the anticlinal walls. This decreased the photothermal signal by increasing the thermal diffusion distance and lowering the light fluence due to greater chloroplast shading. The latter effect increased ES. It appears that chloroplast movement could assist the epidermis to survive harmful light fluences.
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  • 86
    ISSN: 1573-5028
    Keywords: green algae ; high-CO2 stress ; photosynthesis ; regulation ; Rubisco activase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract cDNA and the corresponding genomic DNA region encoding Rubisco activase were isolated from the unicellular green alga Chlorococcum littorale. The deduced amino acid sequence encoded by the cDNA was 403 amino acids long and exhibited important homology with those of other known Rubisco activases. Its N-terminal sequence was similar to the chloroplastic transit peptides in Chlamydomonas reinhardtii. The mature protein had a predicted molecular mass of 42 kDa. Five introns were located inside the genomic gene encoding Rubisco activase (rca). Genomic Southern blots indicated that two copies of the rca gene were present in the genome of C. littorale. The level of rca messenger RNA increased when cells of C. littorale were subjected to high-CO2 stress (i.e. grown under at least 20% CO2). Hsp70 heat-shock protein was also induced under high-CO2 conditions and, as expected, was also induced at 35 °C. The rca gene, in contrast, was not induced at 35 °C, indicating that this gene was induced in response to the high CO2 concentration and not to general stress. A search of the promoter-binding proteins by a gel retardation assay showed that, under the high-CO2 conditions, a protein(s) which was probably an activator of the rca transcription was synthesized.
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  • 87
    ISSN: 1573-5036
    Keywords: calcium ; Coffea arabica ; fluorescence analysis ; nutrient relations ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Calcium deficiency was induced in hydroponically grown 1.5-years-old coffee plants with 12–14 pairs of leaves. Calcium was given in the form of Ca(NO3)2: 5, 2.5, 0.1, 0.01 and 0 mM. After 71 days of Ca-treatment root and shoot as well as total biomass were decreased by severe Ca-deficiency. However, a stronger decrease was observed for shoot growth as revealed by the increase in the root/shoot ratio. New leaves were affected showing decreases in the total leaf area and in Leaf Area Duration (LAD). After 91 days of deficiency, leaf protein concentration decreased (by about 45%) in the top leaves while nitrate reductase activity (NRA) and NO3 content showed no significant changes. Total nitrogen and mineral concentrations (P, K, Ca, Mg and Na) were also determined in leaves and roots. With the decrease in calcium concentration in Ca-deficiency conditions, we observed concomitant increases in the concentrations of K+, Mg2+ and Na+ in leaves (maximal changes of 32% for K+, 96% for Mg2+ and 438% for Na+) and in roots (108% for K+, 86% for Mg2+ and 38% for Na+). Accordingly, the ratio between elements changed, including the ratio N/P, showing a non-equilibrium in the balance of nutrients. Significant correlations were obtained between Ca2+ concentration and some photosynthetic parameters. Ca-deficiency conditions would increase the loss of energy as expressed by the rise in aE and decrease the photochemical efficiency, which confirms the importance of this element in the stabilization of chlorophyll and in the maintenance of good photochemical efficiency at PS II level.
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    Plant and soil 176 (1995), S. 219-227 
    ISSN: 1573-5036
    Keywords: calcium ; chloride ; cotton ; photosynthesis ; potassium ; sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The optimum Ca2+ concentration for growth of cotton (Gossypium hirsutum cv. Acala SJ-2) was in the range 1 to 15 mol m−3 for plants growing in hydroponic culture with 100–150 mol m−3 NaCl. Most saline (but not sodic) soils contain higher Ca2+ concentrations. CaCl2 was inhibitory to the growth of cotton above 20–50 mol m−3. Increasing concentrations of Ca2+ in the range 0–2 mol m−2 drastically reduced Na+ accumulation in the leaves. As CaCl2 concentrations were increased above the optimum for growth there was a further reduction in leaf Na+ accumulation, but this was more than offset by increased leaf Ca2+ and Cl− concentrations. Leaf K+ concentrations were not much affected by changes in external CaCl2 concentrations. The response of Mg2+ varied from an increase to a decrease with increasing external CaCl2 and was influenced by nutritional status. There was no evidence that high Ca2+ caused a deficiency of Mg2+ in cotton. Except for Cl−, whose concentrations tended to decrease initially and then increase as the CaCl2 concentration increased, the anions were largely unaffected by changes in external CaCl2.
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  • 89
    ISSN: 1573-5036
    Keywords: biomass ; growth ; photosynthesis ; salinity ; salt-tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of NaCl salinity on growth, dry-matter production and leaf photosynthesis of seedlings of Eucalyptus camaldulensis Dehnh. and Dalbergia sissoo Roxb. was studied by imposing 4 levels (40, 80, 120 and 160 mM) of NaCl in pot culture. Salinity up to 160 mM did not affect plant survival, but did affect plant growth and dry-matter production depending upon the species and salt concentration. NaCl reduced leaf number and dry-weight of all the plant components, but increased stem dry-weight, especially in E. camaldulensis. Salinization also stimulated total dry-matter production at all the salinity levels in E. camaldulensis but only at 40 mM in D. sissoo. The two species varied in protein and chlorophyll concentration and in leaf photosynthetic rate. Protein and chlorophyll concentration of the plants fell at all the levels of NaCl, except at 40 mM, where stimulation in the photosynthetic carbon assimilation of the plants occurred. However, no distinct relationship between leaf photosynthetic rate and dry-matter production was found. The study indicated that low salt concentrations generally stimulated growth, biomass production and rate of photosynthesis in both the species, and E. camaldulensis appeared more NaCl salt-tolerant than D. sissoo.
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  • 90
    ISSN: 1573-5028
    Keywords: cyanobacteria ; immunogold labelling ; light-harvesting complexes ; photosynthesis ; phycobilins ; phytoplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An intrinsic divinyl-chlorophyll a/b antenna and a particular form of phycobiliprotein, phycoerythrin (PE) III, coexist in the marine oxyphotobacterium Prochlorococcus marinus CCMP 1375. The genomic region including the cpeB/A operon of P. marinus was analysed. It encompasses 10 153 nucleotides that encode three structural phycobiliproteins and at least three (possibly five) different polypeptides analogous to cyanobacterial or red algal proteins involved either in the linkage of subunits or the synthesis and attachment of chromophoric groups. This gene cluster is part of the chromosome and is located within a distance of less than 110 kb from a previously characterized region containing the genes aspA-psbA-aroC. Whereas the Prochlorococcus phycobiliproteins are characterized by distinct deletions and amino acid replacements with regard to analogous proteins from other organisms, the gene arrangement resembles the organization of phycobiliprotein genes in some other cyanobacteria, in particular marine Synechococcus strains. The expression of two of the Prochlorococcus polypeptides as recombinant proteins in Escherichia coli allowed the production of individual homologous antisera to the Prochlorococcus α and β PE subunits. Experiments using these sera show that the Prochlorococcus PEs are specifically associated to the thylakoid membrane and that the protein level does not significantly vary as a function of light irradiance or growth phase.
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  • 91
    ISSN: 1573-5036
    Keywords: pearl millet ; Pennisetum glaucum (L.) R. Br. ; phosphorus ; photosynthesis ; modelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract There have been no studies of the effects of soil P deficiency on pearl millet (Pennisetum glaucum (L.) R. Br.) photosynthesis, despite the fact that P deficiency is the major constraint to pearl millet production in most regions of West Africa. Because current photosynthesis-based crop simulation models do not explicitly take into account P deficiency effects on leaf photosynthesis, they cannot predict millet growth without extensive calibration. We studied the effects of soil addition on leaf P content, photosynthetic rate (A), and whole-plant dry matter production (DM) of non-water-stressed, 28 d pearl millet plants grown in pots containing 6.00 kg of a P-deficient soil. As soil P addition increased from 0 to 155.2 mg P kg−1 soil, leaf P content increased from 0.65 to 7.0 g kg−1. Both A and DM had maximal values near 51.7 mg P kg−1 soil, which corresponded to a leaf P content of 3.2 g kg−1. Within this range of soil P addition, the slope of A plotted against stomatal conductance (gs) tripled, and mean leaf internal CO2 concentration ([CO2]i) decreased from 260 to 92 μL L−1, thus indicating that P deficiency limited A through metabolic dysfunction rather than stomatal regulation. Light response curves of A, which changed markedly with P leaf content, were modelled as a single substrate, Michaelis-Menten reaction, using quantum flux as the substrate for each level of soil P addition. An Eadie-Hofstee plot of light response data revealed that both KM, which is mathematically equivalent to quantum efficiency, and Vmax, which is the light-saturated rate of photosynthesis, increased sharply from leaf P contents of 0.6 to 3 g kg−1, with peak values between 4 and 5 g P kg−1. Polynomial equations relating KM and Vmax, to leaf P content offered a simple and attractive way of modelling photosynthetic light response for plants of different P status, but this approach is somewhat complicated by the decrease of leaf P content with ontogeny.
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  • 92
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    Plant and soil 168-169 (1995), S. 255-261 
    ISSN: 1573-5036
    Keywords: magnesium deficiency ; Norway spruce ; photosynthesis ; Picea abies (L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to investigate the influence of different magnesium nutrition on photosynthesis, one hundred 6-year-old spruce trees derived from one clone were planted in October 1990 into a special out-door experimental construction, where they were cultivated in sand culture with an optimal supply of nutrients, except magnesium, via circulating nutrient solutions. Magnesium was added to the nutrient solutions in three different concentrations, varying from optimal to severe deficient supplies. During the first vegetative period in 1991, photosynthetic performance and carboxylation efficiency were measured under saturating light, controlled CO2 conditions, optimal temperature and humidity, using a minicuvette system. During summer, the trees under moderate magnesium deficiency developed tip yellowing symptoms on older needles, while the youngest needles remained green with unchanged chlorophyll contents. Trees under severe magnesium deficiency showed yellowing symptoms on all needle age classes combined with decreased chlorophyll contents in the youngest needles as well. In comparison with the controls, the photosynthetic performance of the 1-year-old needles was significantly lower in both deficiency treatments. The same was observed in the youngest needles of the trees under severe deficiency. Trees under moderate deficiency treatment decreased in photosynthetic performance during the summer without reduction of chlorophyll contents. The reduction of photosynthetic rates corresponded to a decrease in carboxylation efficiency, which is taken as a measure of the activity of the enzyme ribulose-1,5-bisphosphate carboxylase. This reduction, together with the observed increase of carbohydrate contents in needles of trees growing under magnesium deficiency, led to the assumption that the photosynthetic carbonfixation is reduced as a consequence of the accumulation of carbohydrates.
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  • 93
    ISSN: 1573-5036
    Keywords: ammonium toxicity ; buffered nutrient solution ; eggplant ; flowers ; fruit yield ; nitrate ; photosynthesis ; Solanum melongena ; starch ; sucrose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Eggplants (Solanum melongena L. cv. Bonica) were grown in a glasshouse during summer under natural light with one unbranched shoot or one shoot with 3 to 4 branches and with or without fruit in quartz sand buffered and not buffered with 0.5% CaCO3 (w : v), respectively. Nutrient solutions supplied contained nitrate or ammonium as the sole nitrogen source. Compared with nutrient solutions containing nitrate (10 mM), solutions containing ammonium (10 mM) caused a decrease in net photosynthesis of eggplants during early stages of vegetative growth when grown in quartz sand not buffered with CaCO3. The decrease was not observed before leaves showed interveinal chlorosis. In contrast, net photosynthesis after bloom at first increased more rapidly in eggplants supplied with ammonium than with nitrate nitrogen. However, even in this case, net photosynthesis decreased four weeks later when ammonium nutrition was continued. The decrease was accompanied by epinasty and interveinal chlorosis on the lower leaves and later by severe wilting, leaf drop, stem lesions, and hampered growth of stems, roots, and fruits. These symptoms appeared later on plants not bearing fruits than on plants bearing fruits. If nutrient solutions containing increasing concentrations of ammonium (0.5–30 mM) were supplied after the time of first fruit ripening, shoot growth and set of later flowers and fruits were promoted. In contrast, vegetative growth and reproduction was only slightly affected by increasing the concentration of nitrate in the nutrient solutions. In quartz sand buffered with CaCO3 ammonium nutrition caused deleterious effects only under low light conditions (shade) and on young plants during rapid fruit growth. If eggplants were supplied with ammonium nitrogen before bloom, vegetative growth was promoted, and set of flowers and fruit occurred earlier than on plants supplied with nitrate. Furthermore, the number of flowers and fruit yield increased. These effects of ammonium nutrition were more pronounced when plants were grown with branched shoots than with unbranched shoots. The results indicate that vegetative and reproductive growth of eggplants may be manipulated without causing injury to the plants by supplying ammonium nitrogen as long as the age of the plants, carbohydrate reserves of the roots, quantity of ammonium nitrogen supplied, and pH of the growth medium are favourable. T W Rufty Section editor
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  • 94
    ISSN: 1573-5036
    Keywords: abscisic acid ; Brassica napus ; Brassica carinata ; callus ; photosynthesis ; plant growth ; salinity ; salt tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The greater sensitivity of B. carinata to salinity in comparison to B. napus has been linked to a greater reduction in net assimilation rate. Apparently this is not due to ion toxicity; the cause is unknown. In this report, we test the hypothesis that increases in abscisic acid (ABA) are involved in the reduction of growth by salinity. Salinity (8 dS m−1) caused an increase of ABA concentrations in the shoot, root and callus of both species. ABA concentrations were lower in the salt-tolerant species, B. napus, than the salt-sensitive species, B. carinata, both in the whole plant and callus. Leaf expansion for both species was equally sensitive to ABA; salt stress did not significantly alter sensitivity to applied ABA. The growth inhibition increased in a hyperbolic manner with an increase in endogenous ABA concentration. These results indicate that ABA in salt-stressed plants may play a role in the inhibition of growth. The photosynthesis of salt-sensitive species, B. carinata, was also decreased by salinity, corresponding to the reduction in growth. The decreased photosynthesis does not appear to be the cause of the growth reduction, because photosynthesis was not inhibited by short-term exposure to salinity and photosynthesis was poorly correlated with endogenous ABA concentrations.
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  • 95
    ISSN: 1573-5036
    Keywords: drought ; Glycine max ; photosynthesis ; water use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen fixation in soybean (Glycine max [L.] Merr.) is more sensitive to water deficits than many physiological processes and may therefore limit yield under nonirrigated conditions. Tolerance of nitrogen fixation to water deficits has been observed in the cultivar Jackson, however, the physiological basis for this is unclear. It was hypothesized that genotypes that could continue biomass production on limited soil water would prolong nitrogen fixation by continued photosynthate allocation to nodules. An initial greenhouse experiment compared biomass and N accumulation in six genotypes over an 8 d water deficit. Low stress intensity minimized genotypic expression of water-deficit tolerance; nevertheless, Jackson was clearly one of the most tolerant genotypes. In a second experiment, Jackson was compared to SCE82-303 at more severe stress levels. Biomass and N accumulation continued during water deficits for Jackson but ceased in SCE82-303. Individual nodule mass tended to increase during water deficits in Jackson and tended to decrease in SCE82-303, indicating greater allocation of photosynthate to Jackson's nodules in response to water deficits. Biomass accumulation of Jackson was contrasted with the USDA plant introduction (PI) 416937, which also has demonstrated tolerance to water deficits. For water-deficit treatments, total biomass accumulation was negligible for PI416937, but biomass accumulation continued at approximately 64% of the control treatment for Jackson. Transpirational losses for Jackson and PI416937 were approximately the same for the water-deficit treatment, indicating that Jackson had higher water use efficiency (WUE). Isotopic discrimination of 13C relative to12 C also indicated that Jackson had superior WUE during water deficits. Carbon-14 allocation in Jackson was compared to KS4895, a cultivar that was identified as sensitive to water deficits in an initial experiment. The comparison of water-deficit treatments of Jackson with KS4895 indicated that Jackson exported significantly greater amounts of14 C from labeled leaves and allocated approximately four times greater amounts of 14C per g of nodule. Results indicated that Jackson's sustained biomass production during water deficits resulted in the continued allocation of photosynthate to nodules and prolonged nitrogenase activity.
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  • 96
    Electronic Resource
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    Plant and soil 196 (1997), S. 211-215 
    ISSN: 1573-5036
    Keywords: boron ; deficiency ; function ; membrane ; mobility ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In species in which boron (B) mobility is limited, B deficiency only occurs in growing plant organs. As a consequence of the highly localized patterns of plant growth and the general immobility of B it has been extremely difficult to determine the primary function of B in plants. In species in which B is phloem mobile, the removal of B from the growth medium results in the depletion of B present in mature leaves. Thus, it is possible to develop mature leaves with increasingly severe levels of B depletion, thereby overcoming the complications of experiments based on growing tissues. Utilizing this approach we demonstrate here that B depletion of mature plum (Prunus salicina) leaves did not result in any discernible change in leaf appearance, membrane integrity or photosynthetic capacity even though B concentrations were reduced to 6-8 µg/g dwt, which is less than 30% of the reported tissue B requirement. Boron depletion, however, results in a severe disruption of plant growth and metabolism in young growing tissues. This experimental evidence and theoretical considerations suggest that the primary and possibly sole function of B, is as a structural component of growing tissues.
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  • 97
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 94 (1997), S. 303-309 
    ISSN: 1573-5060
    Keywords: photosynthesis ; hexaploid wheat ; A genome ; D genome ; polyploidy ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We assessed (1) the effects of addition and doses of the D genome from different sources and (2) the addition of either the A genome or the D genome on the photosynthesis of synthesized hexaploid wheats. On average, the increased doses of the D genome reduced photosynthesis, but the depression was dependent on the source of the D genome. Two accessions of Aegilops squarrosa had depressed photosynthetic rates, but not another accession of Ae. squarrosa. The D genome of cv. Thatcher did not contribute to depress photosynthetic rate. Triticum monococcum had considerably higher photosynthetic rates than Ae. squarrosa. However, addition of the A genome from T. monococcum did not increase the photosynthetic rates of hexaploids. Chlorophyll a : b ratio, functional photosystem II and the core complex of photosystem II did not account for the variation in photosynthetic rate among the genotypes studied. In our experiment, photosynthesis of polyploids was not dependent on photosynthesis rates of the donor genomes.
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  • 98
    Electronic Resource
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    Springer
    Photosynthesis research 44 (1995), S. 23-40 
    ISSN: 1573-5079
    Keywords: chloroplasts ; cyanobacteria ; ferredoxin ; photosynthesis ; plastocyanin ; thylakoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosystem I functions as a plastocyanin:ferredoxin oxidoreductase in the thylakoid membranes of chloroplasts and cyanobacteria. The PS I complex contains the photosynthetic pigments, the reaction center P700, and five electron transfer centers (A0, A1, FX, FA, and FB) that are bound to the PsaA, PsaB, and PsaC proteins. In addition, PS I complex contains at least eight other polypeptides that are accessory in their functions. Recent use of cyanobacterial molecular genetics has revealed functions of the accessory subunits of PS I. Site-directed mutagenesis is now being used to explore structure-function relations in PS I. The overall architecture of PSI complex has been revealed by X-ray crystallography, electron microscopy, and biochemical methods. The information obtained by different techniques can be used to propose a model for the organization of PS I. Spectroscopic and molecular genetic techniques have deciphered interaction of PS I proteins with the soluble electron transfer partners. This review focuses on the recent structural, biochemical and molecular genetic studies that decipher topology and functions of PS I proteins, and their interactions with soluble electron carriers.
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  • 99
    ISSN: 1573-5079
    Keywords: diadinoxanthin ; dinoflagellate ; light-harvesting-complex ; peridinin ; photoacclimation ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated Chl a-Chl c-carotenoid binding proteins from the dinoflagellates Prorocentrum minimum and Heterocapsa pygmaea grown under high (500 μmol m−2 s−1, HL) and low (35 μmol m−2 s−1, LL) light conditions. We compared various isolation procedures of membrane bound light harvesting complexes (LHCs) and assayed the functionality of the solubilized proteins by determining the energy transfer efficiency from the accessory pigments to Chl a by means of fluorescence excitation spectra. The identity of the newly isolated protein-complexes were confirmed by immunological cross-reactions with antibodies raised against the previously described membrane bound Chl a-c proteins (Boczar et al. (1980) FEBS Lett 120: 243–247). Spectroscopic analysis demonstrated the relatedness of these proteins with the recently described Chl-a-c 2-peridinin (ACP) binding protein (Hiller et al. (1993) Photochem Photobiol 57: 125–131; Iglesias Prieto et al. (1993) Phil Trans R Soc London B 338: 381–392). The water-soluble peridinin-Chl a binding-protein (PCP) was not detectable in P. minimum. Two functional forms of ACP with different pigmentation were isolated. A variant of ACP which was isolated from high-light grown cells, that specifically binds increased amounts of diadinoxanthin was compared to the previously described ACPs that bind proportionately more peridinin.
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  • 100
    ISSN: 1573-5079
    Keywords: glycine betaine ; osmolyte ; oxygen-evolving complex ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Natural osmoregulatory substances (osmolytes) allow a wide variety of organisms to adjust to environments with high salt and/or low water content. In addition to their role in osmoregulation, some osmolytes protect proteins from denaturation and deactivation by, for example, elevated temperature and chaotropic compounds. A ubiquitous protein-stabilizing osmolyte is glycine betaine (N-trimethyl glycine). Its presence has been reported in bacteria, in particular cyanobacteria, in animals and in plants from higher plants to algae. In the present review we describe the experimental evidence related to the ability of glycine betaine to enhance and stabilize the oxygen-evolving activity of the Photosystem II protein complexes of higher plants and cyanobacteria. The osmolyte protects the Photosystem II complex against dissociation of the regulatory extrinsic proteins (the 18 kD, 23 kD and 33 kD proteins of higher plants and the 9 kD protein of cyanobacteria) from the intrinsic components of the Photosystem II complex, and it also stabilizes the coordination of the Mn cluster to the protein cleft. By contrast, glycine betaine has no stabilizing effect on partial photosynthetic processes that do not involve the oxygen-evolving site of the Photosystem II complex. It is suggested that glycine betaine might act, in part, as a solute that is excluded from charged surface domains of proteins and also as a contact solute at hydrophobic surface domains.
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