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  • Essen : Verl. Glückauf
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  • 1
    ISSN: 1570-7458
    Keywords: Bemisia argentifolii ; Encarsia formosa ; poinsettia ; parasitoids ; nitrogen ; tri-trophic level interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract More wasps of Encarsia formosa Gahan (Hymenoptera: Aphelinidae) were found on fertilized poinsettias, Euphorbia pulcherrima (Willd.) (Euphorbiaceae), than on non-fertilized plants. Parasitization of Bemisia argentifolii Bellows & Perring (Homoptera: Aleyrodidae) by E. formosa was higher on plants treated with calcium nitrate than with ammonium nitrate or on control plants. In a no-choice test, host feeding by E. formosa was higher when hosts were on fertilized plants than when hosts were on control plants. The nitrogen content of whitefly pupae reared on plants treated with ammonium nitrate was higher than those on calcium nitrate-treated plants. Variability in the parasitization of B. argentifolii by E. formosa appears to be due to host plant-mediated differences in the whiteflies. E. formosa may be influenced by the nutritional suitability of the host, which influences whether wasps continue to oviposit, feed, or disperse.
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  • 2
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    Catalysis letters 37 (1996), S. 163-165 
    ISSN: 1572-879X
    Keywords: nitrogen ; Rh(111) ; thermal desorption ; Arrhenius parameters ; lateral interactions ; NO decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Associative desorption of N atoms from the Rh(111) surface is simulated in the framework of the lattice-gas model. The Arrhenius parameters and nearest-neighbour lateral interaction employed to describe the measured thermal desorption spectra are as follows:v=1013 s−1,E d=40 kcal/mol, and ε1=1.7 kcal/mol. The results obtained are used to clarify the role of nitrogen desorption in the NO + CO reaction on Rh(111) atT=400–700 K andP NO≈P CO≈0.01 atm.
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  • 3
    ISSN: 1573-0832
    Keywords: Fusarium ; mycotoxins ; occurrence ; trichothecenes ; wheat ; zearalenone ; deoxynivalenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Wheat for human consumption (140 samples) was collected after harvest from all regions of Bulgaria. The 1995 crop year was characterized by heavy rainfall in the spring and summer months. The internal mycoflora of wheat samples was dominated by Fusarium spp. and Alternaria spp., and storage fungi were rarely present. The samples were analysed for contamination with Fusarium mycotoxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), T-2 Toxin (T-2), diacetoxyscirpenol (DAS), and zearalenone (ZEA), using enzyme immunoassay methods. DON and ZEA were the predominant toxins, with a contamination frequency of 67% and 69%, respectively. The average levels of these toxins in positive samples were 180 μg/kg (DON) and 17 μg/kg (ZEA), maximum concentrations were 1800 μg kg−1 and 120 μg kg−1, respectively. Acetyl derivatives of DON, namely 3-AcDON and 15-AcDON, were found in 2.1 % and 0.7% of the samples, at at maximum level of about 100 μg kg−1. Only one sample was positive for T-2 (55 μg/kg), DAS was not detected. This is the first report about the natural occurrence of a range of Fusarium mycotoxins in wheat for human consumption in Bulgaria.
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  • 4
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    Nutrient cycling in agroecosystems 46 (1996), S. 81-90 
    ISSN: 1573-0867
    Keywords: elemental sulfur ; granule size ; nitrogen ; phosphorus ; potassium ; S oxidation ; sulfur fertilizers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Experiments were undertaken to determine the effect of granule size and nutrients in granulated compound fertilizers fortified with finely divided elemental sulfur (So) on the rate of So oxidation. In one experiment, So was banded together with or apart from triple superphosphate (TSP) while in two others, So was granulated with nutrient and inert carriers. A fourth experiment examined response to S in an So-fortified TSP from a range of granule sizes. Response and, in some cases, So recovery (using 35S labels) by test crops (maize, wheat, upland rice) was measured. In all experiments, P mixed with So increased plant growth and S recovery above treatments in which P and So were physically separated. There was however, no effect of distance of separation on S recovery. In one experiment, N as urea and N and P as diammonium phosphate (DAP) were also found to enhance response to So although to a lesser degree than P alone. These observations were attributed to a nutritional requirement of So-oxidizing microorganisms for P and N. Granulation of So with carriers also influenced oxidation rate, as inferred from the fertilizer S recovery. For a given So concentration, the effect was inversely proportional to the mean diameter of granules. It is shown that this relationship can be explained if one assumes that So particles in granules collapse into a fixed number of aggregates per granule irrespective of granule size when the soluble nutrient carrier dissolves and diffuses away from the point of application.
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  • 5
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    Nutrient cycling in agroecosystems 46 (1996), S. 225-234 
    ISSN: 1573-0867
    Keywords: long-term experiment ; maize ; wheat ; fertilizers ; farm yard manure ; weedicide application ; yield sustainability ; zinc deficiency ; nutrient uptake ; cropping sequence ; organic carbon build-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Long-term field experiments play an important role in understanding the complex interactions of plants, soils, climate and management and their effects on sustainable crop production. A long-term fertilizer experiment with maize-wheat-cowpea (fodder) is in progress since 1971 at Punjab Agricultural University farm Ludhiana, India. The experimental result for the first 21 years showed that application of N alone or in combination with P did not produce as much maize and wheat grains as the application of N, P and K together. Eight years after the start of the experiment, the optimal levels of N, P and K application (100% NPK) were unable to sustain the similar (maize) yield level as before because of Zn deficiency. Whereas in FYM amended plots the Zn deficiency did not appear and the higher crop yields could be sustained. The chemical control of weeds could not sustain the maize productivity at the same level as the manual removal of weeds. It was concluded that the high level of crop production can be sustained with the application of N, P and K under intensive cropping system provided deficiency of any of the micronutrient does not crop up. The deficiency of Zn is most likely to occur in semi-arid light textured alluvial soils under intensive cropping without the addition of farm yard manure/organic manures. In maize based cropping systems, manual control of weeds may be preferred to the chemical one. Addition of FYM in conjunction with 100% NPK is most beneficial both from bio-physical and economic point of view.
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  • 6
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    Nutrient cycling in agroecosystems 46 (1996), S. 53-70 
    ISSN: 1573-0867
    Keywords: crop ; emission ; fertilizer ; nitrogen ; nitrous oxide ; soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This analysis is based on published measurements of nitrous oxide (N2O) emission from fertilized and unfertilized fields. Data was selected in order to evaluate the importance of factors that regulate N2O production, including soil conditions, type of crop, nitrogen (N) fertilizer type and soil and crop management. Reported N2O losses from anhydrous ammonia and organic N fertilizers or combinations of organic and synthetic N fertilizers are higher than those for other types of N fertilizer. However, the range of management and environmental conditions represented by the data set is inadequate for use in estimating emission factors for each fertilizer type. The data are appropriate for estimating the order of magnitude of emissions. The longer the period over which measurements are made, the higher the fertilizer-induced emission. Therefore, a simple equation to relate the total annual direct N2O−N emission (E) from fertilized fields to the N fertilizer applied (F), was based on the measurements covering periods of one year: E=1+1.25×F, with E and F in kg N ha-1 yr-1. This relationship is independent of the type of fertilizer. Although the above regression equation includes considerable uncertainty, it may be appropriate for global estimates.
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  • 7
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    Nutrient cycling in agroecosystems 46 (1996), S. 179-187 
    ISSN: 1573-0867
    Keywords: electrical conductivity ; leaching ; nitrogen ; pH ; phosphorus ; potassium ; release pattern ; slow-release fertilizers ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We studied the effect of temperature on the release of N, P, and K from slow-release fertilizers (SRF). The study was conducted in micro-lysimeters filled with moist peat medium. Increasing the temperature from 4 to 12°C slightly increased N release from three different slow-release N (SRN) carriers with different particle sizes and coating thicknesses. At 21°C the rate of release was significantly different than the other two temperatures. Urea formaldehyde (UF), sulphur coated urea (SCU) and coated calcium nitrate (CCN), incubated in sphagnum moss peat, released between 3 and 20% of the applied N in six weeks. For eight synthetic and organic NPK carriers, the release pattern was similar to UF and SCU. However, the leaching losses of N from the NPK fertilizers were up to twenty times more than for the SRN products. Except for Osmocote® and Duna, which released 30–40% of the applied N as mineral-N within six weeks, all other slow-release and slowly mineralized NPK carriers acted like readily water-soluble compound NPK. Temperature did not affect the nutrient release from NPK fertilizers.
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  • 8
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    Nutrient cycling in agroecosystems 47 (1996), S. 243-250 
    ISSN: 1573-0867
    Keywords: fertilizer value ; nitrogen ; phosphorus ; poultry manure ; urea ; wetland rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Poultry manure applied alone or in combination with urea at different N levels was evaluated as a N source for wetland rice grown in a Fatehpur loamy sand soil. Residual effects were studied on wheat which followed rice every year during the three cropping cycles. In the first year, poultry manure did not perform better than urea but by the third year, when applied in quantities sufficient to supply 120 and 180 kg N ha−1, it produced significantly more rice grain yield than the same rates of N as urea. Poultry manure sustained the grain yield of rice during the three years while the yield decreased with urea. Apparent N recovery by rice decreased from 45 to 28% during 1987 to 1989 in the case of urea, but it remained almost the same (35, 33 and 37%) for poultry manure. Thus, urea N values of poultry manure calculated from yield or N uptake data following two different approaches averaged 80, 112 and 127% in 1987, 1988 and 1989, respectively. Poultry manure and urea applied in 1:1 ratio on N basis produced yields in between the yields from the two sources applied alone. After three cycles of rice-wheat rotation, the organic matter in the soil increased with the amount of manure applied to a plot. Olsen available P increased in soils amended with poultry manure. A residual effect of poultry manure applied to rice to supply 120 or 180 kg N ha−1 was observed in the wheat which followed rice and it was equivalent to 40 kg N ha−1 plus some P applied directly to wheat.
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  • 9
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    Landscape ecology 11 (1996), S. 27-38 
    ISSN: 1572-9761
    Keywords: abandoned channels ; floodplain ; nitrogen ; organic matter ; phosphorous ; potassium ; sediment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nature of sediments in abandoned channels is an important component of their development as floodplain wetlands. The texture, organic matter, phosphorous, potassium, and nitrogen content of sediments were determined for abandoned channels along the Iowa and Cedar Rivers near their confluence in Iowa. Differences in the levels of these constituents were examined among categories of three landscape gradients: present connectivity to the river, time since abandonment, and proximity to agricultural land use. Local scale processes of ecological development are seen in the importance of time for increased organic matter and nitrogen. Basin scale processes of sediment transport and deposition are revealed by the importance of connectivity for decreases in these two elements, and by the counter-intuitive findings for nitrogen and especially phosphorous and potassium in relation to agricultural proximity. Location on a floodplain is important for differentiating development, but it cannot be reduced to univariate gradients.
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  • 10
    ISSN: 1572-9761
    Keywords: wetlands ; non-point source pollution ; nitrogen ; watershed ; leaching ; geographic information system ; landscape ; groundwater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We developed a spatially-explicit, quantitative Nitrogen Leaching Index to assess the potential for non-point source subsurface nitrogen pollution to wetlands. The index was based on the leaching potential of the watershed soils, the amount of nitrogen available for leaching, and the spatial position of nitrogen sources in the watershed. A raster or cell-based geographic information system (GIS) was used to estimate the necessary data inputs for calculating the index, such as soil hydrologic group, land use/soil type combination, groundwater residence time, and location of septic systems. The Total and Average Watershed Nitrogen Leaching Index (TWNLI and AWNLI) were calculated by summing and averaging, respectively, individual cell contributions over a watershed. Analysis of nine wetland watersheds in central New York state, USA, with mixed forest and agricultural land uses illustrated the use of the index for identifying and ranking wetlands with potential nitrogen pollution. Results showed that the spatial characteristics of a watershed potentially can effect subsurface nitrogen delivery to groundwater-dominated wetlands. The use of an index based on watershed soils, topography, and land use may be useful for assessing potential nitrogen pollution to wetlands at a regional scale.
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  • 11
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    Plasma chemistry and plasma processing 16 (1996), S. 577-603 
    ISSN: 1572-8986
    Keywords: Cathode erosion ; electrode materials ; argon ; nitrogen ; hydrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract Cathode erosion continues to be a problem hindering the widespread application of plasma technology. In this work, cathode erosion was studied on titanium, stainless steel 314, copper-nickel 10% and 30%, and copper 122 for magnetically rotated arcs operating in argon, nitrogen, and argon/hydrogen mixtures at a constant magnetic flux density of 0.1 T Titanium and stainless steel gave very low erosion rates in argon (0.2 and 0.3, μg/C respectively). Cupronickels were shown to be suitable for nitrogen and hydrogen plasmas. The slope of hydrogen solubility versus temperature in the cathode material was found to be important in determining hydrogen plasma erosion characteristics. When the plasma gas has a high solubility in the cathode material, or can react with the cathode, a negative erosion rate may result. When gas solubility in the cathode is low, oxide stability and mode of electron emission may govern the erosion rate. A high gas solubility in the cathode material, as with hydrogen, can result in mechanical erosion due to micro-explosions near the cathode surface.
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  • 12
    ISSN: 1573-2932
    Keywords: atmospheric deposition ; ecosystem ; hydrology ; nitrogen ; sulfur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Information on atmospheric inputs, water chemistry and hydrology were combined to evaluate elemental mass balances and assess temporal changes in elemental transport from 1983 through 1992 for the Arbutus Lake watershed. This watershed is located within a northern hardwood ecosystem at the Huntington Forest within the central Adirondack Mountains of New York (USA). Changes in water chemistry, including increasing NO3 − concentrations (1.1 μmol c , L−1 yr-1), have been detected during this study period. Starting in 1991 hydrological flow has been measured from Arbutus Lake and these measurements were compared with predicted flow using the BROOK2 hydrological simulation model. The model adequately (r2=0.79) simulated flow from this catchment and was used to estimate drainage for earlier periods when direct hydrological measurements were not available. Modeled drainage water losses coupled with estimates of wet and dry atmospheric deposition were used to calculate solute budgets. Export of SO4 2− (831 mol c ha−1 yr−1) from the greater Arbutus Lake watershed exceeded estimates of atmospheric deposition in an adjacent hardwood stand suggesting an additional source of S. These large drainage losses of SO4 2− also contributed to the drainage fluxes of basic cations (Ca2+, Mg2+, K+ and Na+). Most of the atmospheric inputs of inorganic N were retained (average of 74% of wet precipitation and 85% total deposition) in the watershed. There were differences among years (56 to 228 mol ha−1 yr−1) in drainage water losses of N with greatest losses occurring during a warm, wet period (1989–1991).
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  • 13
    ISSN: 1573-2932
    Keywords: Pulp and paper mill sludge ; nitrogen ; DOC ; heavy metals ; water quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Primary sludge, secondary sludge, and wood ash from a pulp and paper mill were combined with sand to create a synthetic topsoil (C:N ratio of 18:1) to restore an abandoned gravel pit. Synthetic topsoil was applied to field microcosms at rates equivalent to 0, 2170, 4341, or 6511 kg N/ha; each was seeded with grass. Fifteen chemical constituents in leachate were measured during two field seasons. Cadmium, Ni and Zn were mobilized rapidly by soil disturbance. Chloride and SO4-S eluted rapidly from the sludge along with Na. Nitrate leached with Ca late in each field season when sludge N-mineralization and nitrification exceeded plant uptake and microbial immobilization. Ammonium elution was negligible. Dissolved organic carbon (DOC) was mobilized by decomposition of organic matter in the sludge, as were Mg and K. Copper eluted with DOC, probably as an organic ligand. Lead and ortho-P were below our detection limits. We concluded that a synthetic topsoil with a 30:1 C:N ratio applied at a rate of 2100-4300 kg N/ha should provide adequate plant nutrition while minimizing water quality hazards.
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  • 14
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    Plant molecular biology 30 (1996), S. 1301-1306 
    ISSN: 1573-5028
    Keywords: abscisic acid ; glycine-rich ; ribonucleoprotein ; RNA-binding protein ; RNP motif ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings. whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
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  • 15
    ISSN: 1573-5028
    Keywords: differential display ; heat shock proteins ; non-coding regions ; PCR ; reverse transcription ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific probes from 3′ non-coding regions of different members can assist in the fast retrieval and characterization of individual members of a multigene family. We used the recent technique of differential display for the same purpose. As an example of a multigene family in plants, we selected a heat shock protein gene family, HSP16.9 from wheat, with estimated 12 members. We modified the original differential display technique for selective amplification of the 3′ non-coding regions of different wheat HSP16.9 genes by replacing the random 10-mer in the original method with a conserved HSP16.9 gene family-specific primer. Sixteen cDNA fragments from these experiments were sequenced and they represent 8 different members of a 12 member gene family. Our succes can be attributed to shorter 3′ non-coding regions that are typical of higher-plant genes and use of highly conserved gene family-specific primer in these experiments. This modified differential display technique can be of general application to other plant systems where cloning of the different members of a gene family is desired.
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  • 16
    ISSN: 1573-5028
    Keywords: male sterility ; ovary ; parthenogenetic lines ; pollen allergen ; subtractive hybridization ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To isolate genes specifically expressed at the initiation of plant embryo development we have applied a sensitive subtractive hybridization technique for three isogenic wheat lines of the so-called ‘Salmon system’ with either zygotic or autonomous embryo development. Here we present a gene sequence showing a high homology to grass pollen allergens of type II/III thought to be expressed in pollen tissue only. Surprisingly, the pollen allergen-like sequence, designated Tri a III, is also expressed in gynoecia of the sexual, male fertile wheat line ‘(aestivum)-Salmon’, whereas the two parthenogenetic and male sterile wheat lines ‘(caudata)-Salmon’ and ‘(kotschyi)-Salmon’ completely lack any Tri a III transcript. Our data suggest a positive correlation between the expression of this clone and the manifestation of male fertility. Northern and in situ hybridization analysis revealed that, in addition to its presence in pollen, Tri a III is expressed in the parenchymatous tissue of ‘(aestivum)-Salmon’ ovaries exclusively at the day of anthesis. This precise temporal and spatial expression pattern suggests a more general function of the pollen allergen-like sequence Tri a III not limited to the exhibition of allergens in pollen grains.
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  • 17
    ISSN: 1573-5028
    Keywords: cDNA ; expression ; FKBP ; PPIase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel cDNA encoding for a peptidyl-prolyl-cis-trans-isomerase (PPIase) belonging to the FK506-binding protein (FKBP) family was isolated from wheat. It contains an open reading frame of 559 amino acids and it represents the first plant FKBP-PPIase to be cloned. It possesses a unique sequence which is composed of three FKPB-like domains, in addition to a putative tetratricopeptide repeat (TPR) motif and a calmodulin-binding site. The recombinant FKBP-PPIase expressed in and purified from Escherichia coli exhibits PPIase activity that is efficiently inhibited by the immunosuppressive drugs FK506 and rapamycin. Northern blot analysis showed that wheat FKBP was found mainly in young tissues. Polyclonal antibodies revealed the presence of cross-reacting proteins in embryos, roots and shoots. The unique structural features, the enzymatic activity and the presence of putative isoforms in wheat tissues indicate the possibility of the involvement of wheat PPIase in essential biological functions, similar to other members of the FKBP gene family.
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  • 18
    ISSN: 1573-5028
    Keywords: cDNA cloning ; gene expression ; glutamine synthetase ; grapevine ; nitrogen ; Vitis vinifera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Grapevine (Vitis vinifera L.) glutamine synthetase (GS) was analysed into two distinct classes of isoforms; one of them was present in both leaf and root tissues while the other one showed leaf specificity. Western blot analysis revealed that grapevine GS consists of three types of polypeptides of distinct size and differential tissue specificity. Two structurally distinct cDNA clones, pGS1;1 and pGS1;2, encoding grapevine GS were isolated from a cell suspension library and characterized. Both clones contained open reading frames encoding for polypeptides of 356 amino acids with a predicted molecular mass of about 39 kDa. Although the coding sequences of pGS1;1 and pGS1;2 were 84% similar, their 5′-and 3′-untranslated sequences showed only 40% similarity. The coding sequences of the two clones and the derived amino acid sequences showed higher homology to cytosolic than to chloroplastic GSs of other higher plants indicating that the cDNAs isolated encode for cytosolic isoforms of grapevine GS. Southern blot analysis suggested the existence of more than two GS genes in the grapevine genome. In northern blots both clones were hybridized to mRNAs of about 1.4 kb that are differentially expressed in the various tissues. Supply of nitrate or ammonium in the cell suspension culture medium, as a sole nitrogen source, resulted in differential response of the pGS1;1-and pGS1;2-related genes.
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  • 19
    ISSN: 1432-203X
    Keywords: Triticum aestivum L. ; wheat ; transformation ; biolistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a method for the accelerated production of fertile transgenic wheat (Triticum aestivum L.) that yields rooted plants ready for transfer to soil in 8–9 weeks (56–66 days) after the initiation of cultures. This was made possible by improvements in the procedures used for culture, bombardment, and selection. Cultured immature embryos were given a 4–6 h pre-and 16 h post-bombardment osmotic treatment. The most consistent and satisfactory results were obtained with 30 μg of gold particles/bombardment. No clear correlation was found between the frequencies of transient expression and stable transformation. The highest rates of regeneration and transformation were obtained when callus formation after bombardment was limited to two weeks in the dark, with or without selection, followed by selection during regeneration under light. Selection with bialaphos, and not phosphinothricin, yielded more vigorously growing transformed plantlets. The elongation of dark green plantlets in the presence of 4–5 mg/l bialaphos was found to be reliable for identifying transformed plants. Eighty independent transgenic wheat lines were produced in this study. Under optimum conditions, 32 transformed wheat plants were obtained from 2100 immature embryos in 56–66 days, making it possible to obtain R3 homozygous plants in less than a year.
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  • 20
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    Russian chemical bulletin 45 (1996), S. 794-796 
    ISSN: 1573-9171
    Keywords: nitrogen ; methane ; ethane ; propane ; combined adsorption ; zeolite NaX ; selectivity coefficient
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Adsorption of N2, CH4, C2H6, C3H8, and their mixture on zeolite NaX was studied by the volumetric method under static conditions at 278 K in the pressure range from 0.1 to 0.8 MPa. Compressibility factors were calculated in order to take into account the nonideal character of the gas phase. Adsorption isotherms of individual gases and partial isotherms were obtained. The adsorption properties of gases in the adsorption of a mixture and its components were compared. The selectivity coefficient of adsorption of propane in the N2-CH4-C2H6-C3H8-NaX system was calculated, and its dependence on the total pressure was determined.
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  • 21
    ISSN: 1610-7403
    Keywords: calcium ; mineral distribution ; nitrogen ; potassium ; xylem sap
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The chemical compositions of xylem saps ofCryptomeria japonica D. Don andPinus densiflora Sieb. et Zucc. were studied. One-year-old seedlings of these species were transplanted into hydroponics in the middle of June. Some seedlings were retained in the nursery. Two nitrogen sources, ammonium and nitrate nitrogen, were applied separately. The samplings of xylem saps were made at the end of July. Saps were collected from both roots and tops of the seedlings using a pressure chamber. InC. japonica, the fact that the translocation form of nitrogen from the roots to shoots mainly was citrulline was confirmed. The result ofP. densiflora in this study showed that glutamine was the predominant compound of nitrogen translocation. Total nitrogen ofP. densiflora had a large value in the series of ammonium. Glutamine increased in the ammonium series of both species. Nitrate nitrogen was found in whole samples ofC. japonica, and was not found inP. densiflora. As the result of cations in the saps, monovalent cations were rich inP. densiflora, and divalent cation concentrations were greater inC. japonica. The idea that the great oxygen requirement ofP. densiflora produces the negative charge gives an explanation of the great retention of divalent cations in the roots. The idea that the mineral distribution indicates root activity related to its negative charge production also is considered a possibility.
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  • 22
    ISSN: 1573-5125
    Keywords: Eutrophication ; nutrients ; phosphorus ; nitrogen ; chlorophyll-a ; primary production ; secondary production ; Mytilus edulis ; river Rhine ; Wadden Sea ; North Sea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From 1955 to the mid 1980s the loads of both nitrogen and phosphorus from the river Rhine to the Dutch coastal area, the Wadden Sea included, increased. Since 1985 the phosphorus loads has decreased significantly, while the nitrogen load remained about the same. Annual primary production in the western Dutch Wadden Sea has increased fromc. 40 g C m−2 (1950) to 150 (mid 1960s) and over 500 g C m−2 (1986). The biomass of macrozoobenthos has more than doubled since 1970. Simultaneously, the meat yield of cultured blue mussels (Mytilus edulis), has increased since the 1960s. Previously, it was indicated that the increase in primary production of the phytoplankton over the period 1950 to 1986 was stimulated by the load of dissolved inorganic phosphate from Lake IJssel, a reservoir supplied by Rhine water. Since 1990, however, primary production has been higher than was expected from decreased phosphate loads from Lake IJssel. It is argued that this lack of response may have been caused by increased concentrations of dissolved inorganic phosphate at sea originating from increased inflow from a.o. the Strait of Dover, which compensate for the decrease in phosphate from the rivers, possibly in combination with a significant improvement of the light conditions of the water in the Wadden Sea.
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  • 23
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    Journal of applied phycology 8 (1996), S. 389-396 
    ISSN: 1573-5176
    Keywords: Nitzschia inconspicua ; diatoms ; lipids ; fatty acids ; eicosapentaenoic acid ; silicate ; nitrogen ; carbon source
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of nitrate and silicate levels, and carbon source on growth, biochemical composition and fatty acid composition ofNitzschia inconspicua were investigated using batch cultures. Within the range of silicate levels supplied (8.8–176 μM), no marked variations in growth trend, biochemical composition or fatty acid composition were shown. Biomass at stationary phase, ranging from 64–66 mg ash-free dry weight (AFDW) L−1, and specific growth rate (μ) based on chlorophylla (0.41–0.50 d−1) of the cultures grown within 0.3–3.0 mM NaNO3 were not significantly different. Cultures supplemented with glucose (0.1 % w/v), acetate (0.1 % w/v) or 5% CO2 attained higher biomass (85, 85, 97 mg AFDW L−1) than the control which was grown in synthetic seawater and agitated by magnetic stirring. Cells grown at 〈3.0 mM NaNO3 contained higher carbohydrate contents (14.8–21.5% AFDW) than those grown at 3.0 mM (4.0% AFDW). Lipid content increased at the expense of proteins in cells aerated with 5% CO2. The dominant fatty acids, 16:0 and 16:1, ranged from 35.7–45.0% and 36.4–45.4% total fatty acids (TFA), respectively, while the relative proportions of 20:4 (n-6) and 20:5 (n-3) ranged from 1.7–5.4% and 3.4–5.9% TFA respectively. Cultures aerated with 5% CO2 attained the highest biomass (97 mg AFDW L−1) and yield of 20:5 (n-3) (0.34 mg L−1).
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  • 24
    ISSN: 1618-2545
    Keywords: consecutive planting ; Phoma sp. ; plant growth-promotion ; potting medium ; soybean ; wheat ; zoysiagrass rhizosphere fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several isolates ofPhoma sp., certain nonsporulating fungi, as well asPenicillium andTrichoderma, all isolated from zoysiagrass rhizosphere, promoted growth of wheat and soybean under greenhouse conditions. However, the ability of these rhizosphere fungi to enhance plant growth varied with the crop tested. For example, most of the fungi effectively promoted the growth of wheat, whereas only a few fungi were effective on soybean. In consecutive plantings of wheat and soybean grown in soil previously infested with these zoysiagrass rhizosphere fungi, the growth promotion ability of the fungi was lowered. However, addition of fresh potting medium appeared to restore their growth-promotive effects. It appears that the activation of plant growth-promoting fungi in soil might depend on the availability of organic substrates to colonize, as evidenced by the promotion of plant growth.
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  • 25
    ISSN: 1573-5117
    Keywords: river ; hyporheic ; dissolved organic carbon ; nitrogen ; phosphorus ; silica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Solute, nutrient and bacterial inputs to the River Rhône from the interstitial habitat of a gravel bar and the floodplain aquifer were investigated during an artificial drought. Eight springs were investigated: four groundwater-fed springs in the floodplain, located at the bottom of the bank; and four interstitial-fed springs located at the downstream end of a gravel bar. During this period, the inflows of groundwater to the river represented an average input of 0.77 mg l−1 of nitrogen (of which 93.3% were nitrates), 0.0187 mg l−1 of total phosphorus (of which 42.2% was orthophosphate), 3.56 mg l−1 of silica, 2.315 ± 0.703 mg l−1 of dissolved organic carbon (DOC, of which 47% was biodegradable) and 7.3 × 104 ± 3.7 × 104 bacteria per ml (of which 8.8% were active). Silica, DOC, biodegradable DOC, and bacteria concentrations displayed temporal variations during the study, which seem to be linked to the biological activity of the groundwater biofilm. There was a strong heterogeneity between the two types of groundwater that flow to the river: concentrations of calcium and alkalinity were higher in bank springs than in gravel bars springs. In these latters, sulfate, sodium, nitrogen, phosphorus were significantly higher.
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  • 26
    ISSN: 1573-5117
    Keywords: Sediment ; nitrogen ; phosphorus ; regeneration ; iron availability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During 1994 net sediment-water fluxes of oxygen, ammonium and inorganic phosphorus as well as sediment profiles of organic matter, nitrogen, phosphorus and iron were determined in three shallow eutrophic environments. Investigations were conducted monthly from March to December at five stations in the Sacca di Goro (Po River Delta, Italy). In the late summer, samples were collected from a single site in the Prévost lagoon (French Mediterranean coast) and three stations in the Bassin d'Arcachon (French Atlantic coast). In the Sacca di Goro, water-sediment exchanges of O2, NH 4 + and PO 4 3− were estimated by means of core incubation in the dark. Benthic fluxes for the French lagoons were in part determined experimentally using benthic chambers and in part from the literature. In general in the Sacca di Goro the highest oxygen uptake and nutrient release rates were found at the central sites, affected by macroalgal growth. At the sampling site adjacent to the freshwater inlet, sediment-water exchanges were principally influenced by tidal activity. In terms of organic matter and nutrient levels, sediments from the Sacca di Goro and from the Prévost lagoon, both colonised by the floating macroalga Ulva rigida C. Agardh, were similar. Sediments from the inner sheltered site in the Bassin d'Arcachon, invaded by the rooted macrophyte Ruppia cirrhosa (Pet.) Grande, showed the highest total N and P content (363 ± 157 µmol N cm−3 and 15 ± 2 µmol P cm−3 as average values in the top 10 cm of sediment), but were low in pore water ammonium and orthophosphate probably due to the high sequestering capacity of the system and/or efficient coupling between bacterial nutrient regeneration and assimilation by the plant roots. In addition the outer tidal stations in the Bassin d'Arcachon, invaded by rooted macrophytes, were low in pore water nutrients. A different trend was evident in the Prévost lagoon where the concentrations of exchangeable inorganic phosphorus and ammonium were appreciable (0.28 ± 0.07 µmol P cm−3 and 2.4 ± 1.4 µmol N cm−3 as average values in the top 10 cm of sediment). High amounts of dissolved organic nitrogen were found in the pore water at all the sites investigated showing the key role of the organic nitrogen in the recycling of nitrogen in these systems. The hypothesis that iron is a key factor in controlling phosphorus release is discussed since the Sacca di Goro, which is subject to dystrophic crises, is richer in iron than the Bassin d'Arcachon, which is a more buffered system.
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  • 27
    ISSN: 1573-5117
    Keywords: Nanakita River Estuary ; isotope ratio ; carbon ; nitrogen ; deposit-feeding polychaetes ; sediment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two types of deposit-feeding polychaetes, Neanthes japonica and Notomastus sp., and their surrounding sediments were collected from the Nanakita River Estuary and a small brackish lagoon (Gamõ Lagoon) in northeastern Japan. The samples were examined using stable isotope analysis to assess the site specific feeding mode of the animals and their trophic status. N. japonica is a surface deposit-feeder and Notomastus sp. is a subsurface deposit-feeder. In the estuary, the sedimentary δ 5N tended to become isotopically heavier from the upper estuary (2.0 ∼ 3.9‰) to the river mouth (4.3 ∼ 6.2‰), while sedimentary organic δ 13C constant value (−26.8 ∼ −24.4, average −25.6‰) throughout the river estuary. The δ 13C values of N. japonica were similar to those of the surrounding sediment in the upper estuary, whereas in the lower estuary, N. japonica had a heavier δ 13C value than the surrounding sediment. The δ 13C and dg 15N values indicated that the carbon, but not the nitrogen, of N. japonica was derived from upland plants in the upper estuary. In the lower estuary, a significant fraction of carbon of N. japonica was derived from phytoplankton. Notomastus sp. exhibited heavier δ 13C values than the surrounding surface sediment throughout the estuary and had heavier δ 13C values than N. japonica in the same location. These results suggest selective utilization of sedimentary carbon by those animals following bacterial processing and subsequent fractionation. The difference in δ 15N between sedimentary organics and corresponding polychaetes was 5 ± 1‰ and rather higher than 3.4 ± 1.1‰ expected for normal trophic effects in other animals.
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  • 28
    ISSN: 1573-5117
    Keywords: Ulva rigida ; growth rates ; nitrogen ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth of the seaweed Ulva rigida C. Agardh was investigated in relation to biomass densities, internal nutrient pools and external nutrient supply. Research was carried out from 23 March to 5 July 1994 in the Sacca di Goro (Po Delta, Northern Italy), whose south-eastern part was covered by extensive mats of Ulva rigida. Two types of field experiments were conducted by incubating Ulva thalli inside large cages. In the first experiment, beginning on 23 March, 100 g of wet thalli were placed into the cages, allowed to grow for two weeks, then collected and replaced. This procedure was repeated 8 times over the study period. In the second experiment, Ulva thalli were left inside the cages and collected at selected time intervals (14, 27, 41, 64 and 76 days) in order to simulate the effects of increased density on growth and nutrient storage. We recorded specific growth rates (NGR) ranging from 0.025 to 0.081 d−1 for a period up to two months in the repeated short-term experiments performed at relatively low initial algal densities (300–500 g AFDW m−3). These NGR resulted significantly related to dissolved inorganic nitrogen (DIN) in the water column. Tissue concentrations of total Kjeldahl nitrogen (TN) were almost constant, while extractable nitrate decreased in a similar manner to DIN in the water column. Total phosphorus showed considerable variation, probably linked to pulsed freshwater inflow. In the long-term incubation experiment, NGR of Ulva was inversely related to density. Internal concentrations of both total P and TN reached maximum values after one month; thereafter P concentration remained almost constant, while TN decreased below 2% w/w (by dry weight). The TN decrease was also accompanied by an abrupt decrease in nitrate tissue concentration. The biomass incubated over the two month period suffered a progressive N limitation as shown by a decreasing NY ratio (49.4 to 14.6). The reciprocal control of Ulva against biogeochemical environment and viceversa is a key factor in explaining both resource competition and successional stages in primary producer communities dominated by Ulva. However, when the biomass exceeds a critical threshold level, approximately 1 kg AFDW m−3, the macroalgal community switches from active production to rapid decomposition, probably as a result of selfshading, biomass density and development of anaerobic conditions within the macroalgal beds.
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  • 29
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    Hydrobiologia 334 (1996), S. 169-183 
    ISSN: 1573-5117
    Keywords: Oligochaete ; eutrophication ; large-scale experiment ; ditch ; phosphorus ; nitrogen ; oxygen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eight experimental ditch mesocosms were used to study the effect of eutrophication over four years. The experimental ditches had a sand or clay bottom. The ditches were treated with additions of phosphorus, phosphorus and nitrogen, or without additions (controls). Oligochaetes were sampled by deploying trays with substratum for colonization over twenty weeks. Both the important variables phosphorus, nitrogen and oxygen as well as the oligochaete species and numbers are presented. The effects of nutrient additions on phosphorus, nitrogen and oxygen concentrations were described together with changes in oligochaete species composition and numbers. The results were further analyzed by redundancy analysis (RDA). In the clay-lined ditches nutrient addition coincided with fluctuation in oxygen concentration. The higher the nutrient addition levels the longer the period of oxygen depletion became. During oxygen depletion the number of oligochaetes was strongly reduced or even became zero. The low nutrient status of the sandy bed in the sand-lined ditches slowed down the rate of colonization. Only a few tubificids were collected. Eutrophication effects were only observed at the highest nutrient addition level. Considerable variation is attributed to stochastic factors in the sand-lined ditches. Whether oligochaete species were present was related to the length of the colonization period. The substratum composition and food together with oxygen regime decided whether they become more or less abundant in ditches. Large-scale mesocosm experiments require time to develop. Only after the first colonization period variables of species presences and abundances can be employed to detect changes associated with eutrophication. Oligochaetes can be used to measure colonization as well as eutrophication processes.
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  • 30
    ISSN: 1573-5117
    Keywords: nitrogen ; phosphorus ; chlorophyll ; organic matter ; mountain lakes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Concentrations of total phosphorus (TP), inorganic and organic nitrogen, organic matter, and chlorophyll-a were studied in ten mountain lakes at various stages of acidification, trophy, and type of watershed during each July and October from 1987 to 1990. Concentrations of TP and total organic matter were higher in July than in October. Concentrations of NH44 +-N decreased and NO3 −-N increased from July to October. The relative composition of total nitrogen (TN) and its concentration were strongly dependent on the type of watershed: the lowest TN concentrations were observed in lakes with forested watersheds, increasing above the timberline and reaching maximum values in acidified lakes with rocky watersheds. In the pool of TN, nitrate was most important in lakes above the timberline (70–86% of TN), and organic nitrogen in forest lakes (〉 90% of TN). Lakes with rocky watersheds were characterized by high ratios of TN:TP (〉 250 by mass). The concentration of chlorophyll-a varied widely, from 0.01 to 22.6 µg l−1, without any consistent change between July and October, and were P limited.
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  • 31
    ISSN: 1573-5117
    Keywords: air pollution ; deposition ; sulphur ; nitrogen ; base cations ; throughfall ; Leningrad Region ; Scots pine ; soil water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper presents initial results of a joint Finnish-Russian investigation (1991–1996) of air pollutant deposition in Scots pine (Pinus sylvestris Linné) stands of Karelian Isthmus (north-western Leningrad Region, Russia) and south-eastern Finland. Bulk precipitation and canopy throughfall analyses revealed relatively high sulphur and nitrogen loads in the Karelian Isthmus and in the southern part of south-eastern Finland. The deposition load decreased gradually northwards, the highest levels of S and N deposition in the vicinity of St. Petersburg being two to three times higher than the values considered to be background level in Finland. Besides the acidifying elements, a high calcium deposition was detected in the Karelian Isthmus and in the southern part of south-eastern Finland. The high load of calcium, which probably emanates from oil shale burning in NE Estonia, alleviates the acidifying effect of sulphur and nitrogen deposition.
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  • 32
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    Hydrobiologia 322 (1996), S. 241-248 
    ISSN: 1573-5117
    Keywords: water chemistry ; phosphorus ; nitrogen ; intercalibration ; Lake Ladoga ; large lake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract As part of the Russian-Finnish research studies on Lake Ladoga, joint expeditions were organized in 1992 and 1993. Water samples were collected for intercalibration of chemical analysis methods and to monitor the chemical quality of the lake water. In August of 1992 water samples were taken from northern Lake Ladoga for intercalibration of Russian and Finnish analysis methods. In August 1993 water samples were collected from 23 sampling stations in all parts of the lake; some of these were also used for intercalibration purposes. The oxygen, colour and CODMn results were at the same level in the intercalibration. In 1993, the Ptot results obtained were acceptable. In Ntot, Fe and Mn analysis there seemed to be systematic and random errors between some results. The Secchi depth ranged from 1.5 m to 3.3 m. The average concentrations for the total phosphorus ranged from 15 µg 1−1 to 29 µg 1−1. The total nitrogen values were from 620 µg 1−1 to 690 µg 1−1. The N:P ratio varied from 24 to 40. The concentration of phosphorus indicated mesotrophic or even eutrophic conditions in the lake. Phosphorus seemed to be the limiting nutrient to bacteria and algae.
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  • 33
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    Hydrobiologia 323 (1996), S. 1-8 
    ISSN: 1573-5117
    Keywords: nitrogen ; water chemistry methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied a recently described method for the determination of total nitrogen in natural waters involving sample oxidation with persulfate and subsequent determination of nitrate-nitrogen with second derivative spectroscopy and compared it to the USEPA approved method involving the sum of nitrate-nitrogen and Kjeldahl-nitrogen as measured with an automated analyzer. The overall objective was to determine if the two methods gave the same estimates of total nitrogen and if the detection limits, precision and accuracy of the new method were as good as those of the USEPA method. The new method was used to make measurements on replicated blanks, standards and lake water samples covering a range of concentrations. We also collaborated with certified laboratories to make comparative measurements on 5 standards and 21 lake water samples that were run by us with the new method and by them with the USEPA method. The new method had an instrument detection limit of 0.07 mg 1−1, and the standard deviation of 20 sets of lake water samples averaged 0.03 mg 1−1. The new method gave concentrations equivalent to those found with the USEPA method, was more sensitive, and had a higher degree of precision. We concluded that the new method is suitable as a substitute for the USEPA method. We also found that the addition of acid to lake water samples stored under refrigeration was not necessary to preserve them for later determinations of nitrate-nitrogen and total nitrogen and that freezing was an effective means of sample preservation for 90 days.
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  • 34
    ISSN: 1573-5117
    Keywords: Leaf decomposition ; polyphenols ; nitrogen ; microbial colonization ; Eucalyptus globulus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Leaf decomposition of the exotic evergreen Eucalyptus globulus (eucalyptus), and three native deciduous tree species, Alnus glutinosa (alder), Castanea sativa (chestnut) and Quercus faginea (oak), was compared in a second order stream in Central Portugal. Changes in dry weight, nitrogen and polyphenolic compounds and microbial colonization were periodically assessed for three months. Negative exponential curves fit the leaf weight loss with time for all leaf species. Mass loss rate was in the order alder (K = 0.0161) 〉 chestnut (K = 0.0079) 〉 eucalyptus (K = 0.0068) 〉 oak (K = 0.0037). Microbial colonization followed the same pattern as breakdown rates. Evidence of fungal colonization was observed in alder after 3 days in the stream, whereas it took 21 days in oak leaves to have fungal colonization. Fungal diversity was leaf species-dependent and increased with time. In all cases, percent nitrogen per unit leaf weight increased, at least, at the initial stages of decay while soluble polyphenolics (expressed as percentage per unit leaf weight) decreased rapidly in the first month of leaves immersion. Intrinsic factors such as nitrogen and polyphenolic content may explain differences in leaf decomposition. The possible incorporation of eucalyptus litter into secondary production in a reasonable time span is suggested, although community balance and structure might be affected by differences in allochthonous patterns determined by eucalyptus monocultures.
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  • 35
    ISSN: 1573-5117
    Keywords: biofilter ; nitrogen ; Sparus ; Ulvia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ulva rigida was cultivated in 7501 tanks at different densities with direct and continuous inflow (at 2, 4, 8 and 12 volumes d−1) of the effluents from a commercial marine fishpond (40 metric tonnes, Tm, of Sparus aurata, water exchange rate of 16 m3 Tm−1) in order to assess the maximum and optimum dissolved inorganic nitrogen (DIN) uptake rate and the annual stability of the ‘Ulva tank biofiltering system’. Maximum yields (40 g DW m−2 d−1) were obtained at a density of 2.5 g FW 1−1 and at a DIN inflow rate of 1.7 g DIN m−2 d−1. Maximum DIN uptake rates were obtained during summer (2.2 g DIN M−2 d−1), and minimum in winter (1.1 g DIN m−2 d−1) with a yearly average DIN uptake rate of 1.77 g DIN m−2 d−1 At yearly average DIN removal efficiency (2.0 g DIN m−2 d−1, if winter period is excluded), 153 m2 of Ulva tank surface would be needed to recover 100% of the DIN produced by 1 Tm of fish.
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  • 36
    ISSN: 1573-6849
    Keywords: in situ hybridization ; intermediate wheatgrass ; translocation ; virus resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescencein situ hybridization (FISH) was used to determine the breakpoint of the translocation chromosome in two bread wheat (Triticum aestivum) germplasm lines withThinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, genome-specific random amplified polymorphic DNA (RAPD) markers were used to ascertain the genomic sources of theTh. intermedium chromosomes carrying the WSMV or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from induced homoeologous pairing by using theph1b mutant, had a translocation chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, and the entire 4ES ofTh. intermedium. The BYDV-resistant translocation line, TC14, derived from tissue culture, had a very short distal segment of 7StL fromTh. intermedium terminally attached to 56% of the proximal 7DL. These observations indicate that translocations in these wheat germplasm lines did not involve centromeric breaks and fusion but were a result of homoeologous chromosome recombination.
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  • 37
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    Wetlands ecology and management 4 (1996), S. 199-206 
    ISSN: 1572-9834
    Keywords: constructed wetlands ; wastewater ; macrophytes ; nitrogen ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first full-scale constructed wetland (CW) in the Czech Republic was built in 1989 and there are now three tertiary systems and 50 secondary treatment facilities. We report here on the design and operational efficiencies of these facilities. All CWs have been designed with horizontal subsurface flow. Coarse sand, gravel and crushed stones with size fraction of 4–16 mm are commonly used as substrates. The area of vegetated beds ranges between 18 and 4500 m2 and operational CWs are designed for population equivalent (PE) of 4 to 1,100. Common reed (Phragmites australis) is the most frequently used macrophyte species. Results from systems studied during 1994 and 1995 show that the effluent concentrations of organics and suspended solids (SS) are well below the required discharge limits. In most cases the final effluent BOD5 concentration is 〈10 mg l−1. The relationship between vegetated bed BOD5 inflow loadings (L 0) and outflow loadings (L) is very strong (r=0.92). Constructed wetlands with subsurface horizontal flow usually do not remove larger amounts of nitrogen and phosphorus. The results from five Czech constructed wetlands show that nitrogen removal varies among systems, but the amount of removed nitrogen is very predictable. A regression equation between nitrogen inflow loading (L 0) and outflow loading (L) produces a strong correlation (r=0.98). The most important process responsible for phosphorus removal in wetlands is precipitation with soil Ca, Fe and Al. However, the subsurface horizontal flow constructed wetlands use mostly coarse gravel and/or sandy materials and this provides little or no P precipitation. Results from monitored systems in the Czech Republic show that the percentage phosphorus removal varies widely among systems and is lower than the percentage removal of organics and suspended solids.
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  • 38
    ISSN: 1572-9834
    Keywords: Alnus glutinosa ; constructed ecosystems ; disinfection ; Iris pseudacorus ; multi-stage systems ; nitrogen ; phosphorus ; constructed treatment wetlands ; optimization ; purification efficiencies ; urban wastewaters treatment ; Typha latifolia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tests were carried out under controlled conditions in the Experimental Plant of Viville (Arlon, Belgium) to enhance the purification of urban wastewater by “natural” means. The results demonstrate the need to structure treatment systems in a series of different artificial ecosystems (or a Hierarchical Mosaic of Artificial Ecosystems — MHEA in French). The first two levels we used were made up of an unplanted aquatic ecosystem (stabilization pond) followed by a semi-aquatic ecosystem planted withTypha latifolia L. in which the water flows over the substrate. At a flow rate of 4 m2/PE (1 PE=150 1/day of typical urban wastewaters in Belgian rural zones), this first stage substantially reduces suspended solids (SS), COD and BOD5, a significant amount of tot-N and tot-P, and reduces pathogens by 100-fold. Further, the system is easy to manage (sludge is eliminated in the first stage and biomass is collected in the second stage) and the treatment system does not clog up. Nevertheless, real and sustainable environmental protection demands even higher performance rates, and these first two stages, both in terms of design and dimension, can only be considered as a satisfactory part of a MHEA system. Artificial aquatic, semi-aquatic, and terrestrial ecosystems were systematically compared at the third and fourth stage of the system to increase the overall removal efficiency. The most complete and efficient system in our tests (i.e., the one that provides the most successful primary (SS), secondary (COD and BOD5) and tertiary (N and P) treatment and the best pathogens removal rates) was made up of 3 sequential series of ecosystems: an aquatic ecosystem whose flow went into a plantedTypha latifolia system (surface water flow), that flowed into a terrestrial ecosystem planted withAlnus glutinosa (L.) Gaertn (vertical subsurface water flow). A total surface area (stages 1–4) of 8 m2/PE ensured a high performance level whose outflow conformed to the strictest European norms.
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  • 39
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    Plant and soil 184 (1996), S. 281-289 
    ISSN: 1573-5036
    Keywords: chlorosis ; growth ; iron ; nitrogen ; phosphate ; soil acidity ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Veronica officinalis and Carex pilulifera, widespread plants of acid soils in Europe, were grown in 50 soils of natural and seminatural ecosystems representing a wide range of soil chemical properties. The experiment was performed in a greenhouse at a soil moisture content of 55–65% WHC, ca. 60% R.H. of the air, temperature 14–16°C at night and 19–21°C by day; additional light 70 W m-2 12 h d-1. Properties closely related to soil acidity precluded growth of V. officinalis and limited the growth rate of C. pilulifera at soil pH-KCl 〈 ca. 3.4. In slightly-moderately alkaline (calcareous) soils, growth was primarily limited by insufficient phosphate uptake. A low growth rate of C. pilulifera. in such soils was related to low concentrations of exchangeable soil phosphate and low tissue concentrations of phosphorus. However, in high-pH soils, secondary effects due to suboptimum trace element (probably Fe) conditions, giving rise to symptoms of chlorosis, were also indicated. The highest growth rates of both species were invariably measured in soils of intermediate acidity having very high concentrations of exchangeable phosphate. Multiple regression tests on the entire material indicated that 65–75% of the variability in several growth functions could be accounted for, when two or more soil characters were included in the equation. Besides phosphate, exchangeable Zn (in C. pilulifera) and nitrate (in V. officinalis) were of considerable importance in accounting for growth rates.
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  • 40
    ISSN: 1573-5036
    Keywords: ammonium ; heterogeneity ; inflow ; localised nutrient ; mineralisation ; 15N ; nitrate ; organic residue ; proliferation ; roots ; soil ; uptake ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To obtain nutrients mineralised from organic matter in the soil, plants have to respond to its heterogeneous distribution. We measured the timing of nitrogen uptake by wheat from a localised, 15N labelled organic residue in soil, as well as the timing of changes in root length density. We calculated the rates of N uptake per unit root length (inflows) for roots growing through the residue and for the whole root system. A stimulated local inflow appeared to be the main mechanism of exploitation of the residue N during the first five days of exploitation. 8% of the N that the plants would ultimately obtain from the residue was captured in this period. Roots then proliferated in the residue. This, together with a rapidly declining N inflow, contributed to the capture, over the next seven days, of 63% of the N that the plants derived from the residue. After that time, massive root proliferation occurred in the residue, but relatively little further N was captured.
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  • 41
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    Plant and soil 179 (1996), S. 45-56 
    ISSN: 1573-5036
    Keywords: Eucalyptus regnans ; nitrogen ; phosphorus ; soil desiccation ; soil microfractures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The poor growth of young Eucalyptus regnans seedlings in undried soil from the mature forest of E. regnans can be overcome by previously air-drying the soil or by adding sufficient amounts of complete soluble fertilizer or equivalent concentrations of P (as NaH2PO4) and N (as NaNO3). A factorial pot experiment in which phosphate and nitrate were added to undried soil indicated that P was the primary deficiency for young seedlings and that response to N did not occur until this lack was satisfied. In dried soil, seedlings also responded to additions of complete fertilizer but most of this effect was due to N rather than P. Field trials in the mature forest also indicated greater growth in dried soil than undried soil and confirmed a response of young seedlings to superphosphate. In pot experiments, the concentration of P and N per g plant dry weight after four months was relatively constant irrespective of the final size of the plant. Seedlings in dried soil extracted up to 15 times more P than did those grown in undried soil. In general, chemical analysis of soil indicated more extractable P and N from dried soil although this was not always consistently so. Soil desiccation resulted in an increase in soil surface area due to the fragmentation of larger peds and to an increase in the number of microfractures which remained in the soil crumbs after rewetting. Mycorrhiza are likely to be important since the differentiation of the growth response of seedlings in dried and undried soil, which occurred at 5–6 weeks, corresponded with the establishment of full ectomycorrhizal development (80% root tips). The factors concerned with the increase in fertility after air-drying are discussed.
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  • 42
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    Plant and soil 181 (1996), S. 83-93 
    ISSN: 1573-5036
    Keywords: amino sugars ; fixed NH4 + ; immobilization ; mineralization ; nitrogen ; proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Major known fractions of soil nitrogen are amino nitrogen (proteins, peptides), polymers of amino sugars, and NH4 + fixed in interlayers of 2:1 minerals. Only a small percentage of the total soil organic N is easily mineralizable and contributes to the pool of mineral soil N. Predominant sources of mineralization are amino-N and polymers of amino sugars present in the soil microbial biomass. Influx into this pool occurs with the application of organic matter (green manure, straw), organic carbon released by plant roots, N2 assimilation by leguminous species and inorganic nitrogen. Microbial metabolization of green manure proteins results in a partial mineralization of the applied organic N, microbial metabolization of straw in the assimilation (immobilization) of inorganic nitrogen. Microbial biomass is characterized by a narrow C/N ratio (proteins, peptidoglycans, polymers of amino sugars). Its metabolization therefore is associated with a partial mineralization of the attacked organic nitrogen compounds. Nitrogen mineralization consists of a sequence of enzymatic processes for which the living microbial biomass provides the enzymes and the dead microbial biomass the substrate.
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  • 43
    ISSN: 1573-5036
    Keywords: evapotranspiration ; grain yield ; irrigation ; plant density ; water use efficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Yield of eight wheat cultivars was evaluated under rainfed and irrigated conditions in a Mediterranean environment. Variation in grain yield resulted from variation in both aboveground biomass production and in harvest index. Under rainfed compared to irrigated conditions, grain yield, biomass and days to heading were decreased, whereas harvest index was increased. Grain yield of the different cultivars under rainfed conditions correlated with that under irrigated conditions in one of the two years. Among cultivars, harvest index under rainfed and irrigated conditions were correlated in both years. Water was used more efficiently for biomass production, and equally efficiently for grain production, under irrigated compared to rainfed conditions. Under rainfed conditions, crop water use efficiency was higher for cultivars developed for rainfed environments than for those developed for high-rainfall or irrigated environments. Cultivars with low-rainfall target environments had the lowest evapotranspiration under rainfed conditions. Under rainfed conditions, differences between the cultivar groups in crop water use efficiency corresponded with trends in water use efficiency of individual plants and with the ratio of photosynthesis to transpiration, measured on plants grown in a growth room. Early in the season, water was used more efficiently for biomass production at high sowing densities than at low sowing densities. Through faster biomass production and ground cover a smaller proportion of the evapotranspired water was lost in soil evaporation and a larger proportion was transpired. However, the net effect was a greater water use in the early phases of growth and consequently a lower water availability later in the season, leading to similar yields regardless of sowing density.
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  • 44
    ISSN: 1573-5036
    Keywords: crown rot ; Fusarium ; fungal disease ; genotypes ; wheat ; zinc ; zinc efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A crown rot disease in wheat caused by the fungusFusarium graminearum Schw. Group 1 is a widespread problem in chronically Zn-deficient Australian soils. A link between crown rot and Zn deficiency was established by Sparrow and Graham (1988). This paper reports a test of a further hypothesis, that wheat genotypes more efficient at extracting zinc from low-zinc soils are more resistant to infection by this pathogen. Three wheat cultivars (Excalibur, Songlen and Durati) of differential Zn efficiency were tested at three zinc levels (0.05, 0.5 and 2.0 mg Zn kg−1 of soil) and three levels ofF. graminearum S. Group 1 inoculum (0.1 g and 0.3 g kg−1 live chaff-inoculum and control having 0.1 g kg−1 dead chaff inoculum). Six weeks after sowing dry matter production of shoots and roots was decreased byFusarium inoculation at 0.05 mg and 0.5 mg kg−1 applied Zn.Fusarium inoculum at 0.1 g was as effective as 0.3 g kg−1 for infection and decreasing dry matter. The infection at the basal part of culm decreased significantly by increasing the rate of Zn application. Excalibur, a Zn-efficient cultivar (tolerant to Zn deficiency) produced significantly more shoot and root dry matter, and showed less disease infection compared with Zn-inefficient cultivars (Durati and Songlen) at low (0.05 mg Zn kg−1 soil) and medium (0.5 mg Zn kg−1 soil) Zn fertilization rates. Higher rate of Zn fertilization (2.0 mg Zn kg−1 soil) reduced the disease level in Durati to the level of Excalibur but the disease level of Songlen was still high, indicating its high Zn requirement and or sensitivity to crown rot. The data on Zn uptake show that Excalibur, being Zn-efficient, was able to scavenge enough Zn from Zn-deficient soil, we suggest that besides sustaining growth Excalibur was able to build and maintain resistance to the pathogen; inefficient cultivars needed extra Zn fertilization to achieve performance comparable to that of Excalibur. The present study indicates that growing Zn-efficient cultivars of wheat along with judicious use of Zn fertilizer in Zn-deficient areas where crown rot is a problem may sustain wheat production by reducing the severity of the disease as well as by increasing the plant vigour through improved Zn nutrition. ei]Section editor: R Rodriques-Kalana
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  • 45
    ISSN: 1573-5036
    Keywords: ectomycorrhiza ; fungus-specific compounds ; isocitrate dehydrogenase ; nitrogen ; Picea abies ; phosphoenolpyruvate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The response of carbohydrate metabolism in 3-year-old Norway spruce plants to an increased amount of nitrogen supply to a N-poor forest soil was investigated in a pot experiment. After 7 months of treatment we found a decreased amount of starch in both needles and roots, together with decreased amounts of sucrose in needles of those plants grown under an enhanced inorganic N supply. In addition, the activity and the protein amount of the anaplerotic enzyme phosphoenolpyruvate carboxylase (PEPC) and the activity of NADP-dependent isocitrate dehydrogenase (IDH) were clearly increased. The activity of sucrose phosphate synthase (SPS) and the pool size of fructose 2,6-bisphosphate (F26BP) were not affected by high supply of inorganic N. These data indicate a shift of carbon flow from starch formation towards an enhanced provision of carbon skeletons for N assimilation and shoot growth. In parallel, we found decreased contents of fungus-specific compounds (ergosterol, mannitol, trehalose) in roots, which are indicators of a decreased colonization by ectomycorrhizal fungi, probably as a result of a changed allocation and partitioning of photoassimilates due to an increased N supply.
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  • 46
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    Euphytica 88 (1996), S. 165-174 
    ISSN: 1573-5060
    Keywords: wheat ; tritordeum ; Hordeum chilense ; Fusarium culmorum ; Septoria nodorum ; scab ; deoxynivalenol ; ergosterol ; resistance ; Triticum spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae. It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum. Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense, followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases.
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  • 47
    ISSN: 1573-5060
    Keywords: Aegilops markgrafii ; aneuploids ; chromosome specific library ; DOP-PCR ; microdissection ; molecular markers ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We established a chromosome specific DNA library of the Aegilops markgrafii chromosome B. Eight microdissected chromosomes B obtained from a monosomic T. aestivum-Aegilops markgrafii addition line were PCR-amplified and the DNA was cloned in Escherichia coli DH5α. Clones were characterized by dot blot hybridization with total Ae. markgrafii DNA. 62% of clones represented repetitive sequences and 38% low or single copy sequences. The estimated length of excised inserts varied between less than 200 bp and more than 500 bp. The average size of inserts was 310 bp.
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  • 48
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    Euphytica 89 (1996), S. 1-10 
    ISSN: 1573-5060
    Keywords: aneuploidy ; chromosome substitutions ; height ; wheat ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The difficulties in developing inter-varietal chromosome substitution lines in wheat are reviewed. The use of genetical, cytological and molecular markers is suggested as a way of overcoming them. These difficulties and the use of markers, as well as the need to develop duplicate lines to detect background variation, are described using the development and analysis of the Cappelle-Desprez (Bezostaya 1) chromosome substitution set as an example. The effects of substituting Bezostaya 1 chromosomes on final plant height and adult-plant resistance to yellow rust are reported. The large number of aneuploids and substitution lines available in wheat provides a tremendous, international resource, which should be exploited in the future.
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  • 49
    ISSN: 1573-5060
    Keywords: Bread-making quality ; chromosome effects ; grain hardness ; SDS-sedimentation volume ; substitution lines ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The group 1 and 6 inter-varietal chromosome substitution lines of Cappelle-Desprez (Bezostaya 1) were intercrossed along with the donor and recipient varieties, Cappelle-Desprez and Bezostaya 1, to give 36 genetically different families. The analysis of the means of these families showed that variation in SDS-sedimentation volume fitted a predominantly additive model. There were no significant within or between chromosome interactions among the group 1 and 6 chromosomes. Nor was there any evidence for interactions between these chromosomes and those of the background. Significant dominance/within chromosome interactions amongst the background chromosomes were however detected. Some of the positive effects on SDS-sedimentation were associated with increased grain hardness. Chromosome effects on % grain protein were not correlated with SDS-sedimentation.
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  • 50
    ISSN: 1573-5060
    Keywords: agronomic characters ; QTL ; RFLP maps ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The advent of molecular marker systems has made it possible to develop comparative genetic maps of the genomes of related species in the Triticeae. These maps are being applied to locate and evaluate allelic and homoeoallelic variation for major genes and quantitative trait loci within wheat, and to establish the pleiotropic effects of genes. Additionally, the known locations of genes in related species can direct searches for homoeologous variation in wheat and thus facilitate the identification of new genes. Examples of such analyses include the validation of the effects of Vrn1 on chromosome 5A on flowering time in different crosses within wheat; the indication of pleiotropic effects for stress responses by the Fr1 locus on chromosome 5A; the detection of homoeologous variation for protein content on the homoeologous Group 5 chromosomes; and the detection of a new photoperiod response gene Ppd-H1 in barley from homoeology with Ppd2 of wheat.
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  • 51
    ISSN: 1573-5060
    Keywords: aneuploids ; automated DNA sequencer ; chromosomal location ; microsatellite marker ; PCR ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chromosomal assignment of 64 PCR-amplified microsatellite loci and 29 additional fragments amplified by the same primer pairs is described for bread wheat (Triticum aestivum). The distribution over the different chromosomes and chromosome arms appears to be random. The highest proportion of microsatellite loci is found on the B genome, followed by the A and D genome. About half of the primer pairs amplified unique fragments, while the other half amplified additional fragments. 25% of the primer pairs, mostly designed to clones of a PstI-library, amplify fragments on homoeologous chromosomes. In some cases, more than one fragment on a single chromosome or fragments on non-homoeologous chromosomes occurred. The use of an automated DNA sequencer accounts for the accurate resolution of multiple fragments and enables to differentiate between fragments, amplified by a single primer pair, with size differences as small as two base pairs.
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  • 52
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    Euphytica 89 (1996), S. 49-57 
    ISSN: 1573-5060
    Keywords: adaptability ; earliness per se ; photoperiod sensitivity ; vernalization sensitivity ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In order to obtain high levels of environmental adaptability in wheat varieties it is essential they flower at times appropriate to particular environmental conditions. The influence of three distinct genetic systems that together determine time of flowering is reviewed here. Vernalization genes are seen to be particularly important to winter wheats for their direct or indirect effects on winter hardiness. Vernalization genes play a minor role in determining flowering time in autumn sown winter wheats but insensitivity is essential if spring sown wheats are to flower. Day length sensitive photoperiod genes play a major role in determining flowering time and adaptability of autumn sown wheats. Insensitivity can promote yield advantages of over 35% in Southern European environments. 15% in Central Europe and offers benefits even in the UK. At present only a single allele of Ppd1 appears to have been introduced into commercial European wheat varieties. The merits of alternative Ppd1 alleles or different loci are discussed. The influence of earliness per se genes that determine flowering time independently of environmental stimuli is less well documented than the effect of photoperiod and vernalization genes. It is likely that genes on chromosomes belonging to groups 2, 3, 4, 6 and 7 may act to modify flowering time independently of environmental stimuli probably by determining numbers of vegetative and floral primordia being initiated or the rate of initiation of the primordia. Earliness per se genes appear to be widespread in European wheats and play a significant role in determining the exact time plants flower.
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  • 53
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    Euphytica 89 (1996), S. 69-75 
    ISSN: 1573-5060
    Keywords: dwarfing genes ; gene mapping ; GA insensitivity ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The improvement of lodging resistance by introducing major dwarfing genes, classified either as GA insensitive or GA sensitive, is one of the main strategies chosen by cereal breeders. In the present paper the current knowledge about the genetics, chromosomal localisation and the homoeoallelic relationships of the dwarfing genes in wheat and rye is reviewed. The confusing system of the symbolisation of the GA insensitive dwarfing genes/alleles in wheat is discussed and a nomenclature based on rules for gene symbolisation in wheat is proposed.
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  • 54
    ISSN: 1573-5060
    Keywords: Chromosomal location ; grain size ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Grain size in wheat is the most stable yield component and has a favorable effect on flour yield. To identify the chromosomes associated with the large grains of line G603-86, (grain weight over 60 mg and grain length of about 9 mm), F3 lines, extracted from F2 populations obtained from F1 monosomics of crosses between G603-86 (P1) and the monosomic set of Favorit (P2) were tested in the field. ANOVA showed significant differences among parents for grain weight and grain length, but not for grain width or the factor expressing the difference in grain form and density. Homoeologous groups had significant effects on grain weight and on all components of grain weight, while genomes were not significantly different for any of these characters. Grain weight was significantly increased by chromosomes 6D and 4A of G603-86. Grain length was significantly increased by chromosomes 4A, 4B, 2B, 3A and 1B, grain width by chromosomes 1A and 1B, and the factor form-density by chromosomes 6D and 6A. The high grain size in G603-86 results from the effects of genes located on many chromosomes which affect grain dimensions, form and density.
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  • 55
    ISSN: 1573-5060
    Keywords: chromosome substitutions ; hybrid dwarfness ; photoperiodic response ; tissue culture response ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The wheat varieties Chinese Spring, Marquis and Thatcher and five intervarietal ‘Chinese Spring’ substitution lines for chromosomes 2B (2 lines), 2D (2 lines) and 4A*, differing from the recipient variety in alleles for hybrid dwarfing genes and/or the photoperiodic response genes were analysed for tissue culture response (TCR). It could be demonstrated that only chromosome 2B has a major effect on TCR. Allelic variation at the hybrid dwarfing loci seems to have no effect on tissue culture performance, even in the combination D1D2D3 which gives the grass dwarf phenotype. Also the allelic constitution at the Ppd loci, gave no indication for a direct major effect of those alleles, however there seems to exist genetical factors for TCR on the homoeologous group 2 chromosomes which may be closely linked to the Ppd loci.
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  • 56
    ISSN: 1573-5060
    Keywords: Triticum miguschovae ; wheat ; leaf rust resistance ; transfer lines ; monosomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Triticum miguschovae, a genome addition synthetic, was used as a source for transfer of leaf rust (Puccinia recondita tritici) resistance to common wheat. This synthetic, developed from two wild species Triticum militinae and Aegilops squarrosa, proves a valuable donor of the genes for leaf rust resistance. Leaf rust resistance was transferred from T. miguschovae by both dominant and recessive genes. Stable lines phenotypically similar to their recurrent parents Kavkaz and Bezostaya 1 but differing from them in a high level of leaf rust resistance were obtained. The genes for resistance in 3 selected lines differed from each other and from the known effective genes Lr9, Lr19, and Lr24. The resistance of one of them (line 1229) is controlled by two complementary interacting genes located on chromosome 7B and 1D was revealed by monosomic analysis.
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  • 57
    ISSN: 1573-5060
    Keywords: Aneuploidy ; dwarfing genes ; Triticum aestivum ; preferential transmission ; Aegilops sharonensis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat varieties tend to be chromosomally unstable producing on average 2–3% of plants with abnormal chromosome numbers. A number of semi dwarf wheat varieties, carrying the gibberellic acid insensitive dwarfing genes Rht1 or Rht2, have been seen to produce distinct tall off types due to reduction in dosage of the chromosome carrying the dwarfing gene. The UK variety ‘Brigand’, carrying Rht2 on chromosome 4D, produced very distinct tall off types when this chromosome was reduced in dosage. The frequency of tall off types was sufficiently high to cause the variety to fail United Kingdom statutory uniformity tests. An attempt to prevent the loss of chromosome 4D was made by constructing translocation chromosomes involving the short arm of chromosome 4D, which carries Rht2, and the long arm of chromosome 4S l from Aegilops sharonensis, which carries a gene(s) conferring preferential transmission. The work in this paper describes the field evaluation of two lines carrying 4DS.4DL-4S l L translocations, and demonstrates their success in preventing spontaneously occurring monosomy of chromosome 4D in semi-dwarf wheats.
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  • 58
    ISSN: 1573-5060
    Keywords: C-banding ; FISH ; heterochromatin ; in situ hybridization ; rye ; Secale cereale ; Secale montanum ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The sequential combination of C-banding and in situ hybridization techniques applied in this or in a reverse order, are used to recognize targeted chromosomal regions in cereals. Both methods are described whereby standard chromosome squash preparations are followed by: i) C-banding technique using Leishman stain and a slightly modified in situ hybridization technique using biotin-labeled DNA probes, or ii) fluorescence in situ hybridization technique and C-banding. Both approaches have been successfully used onto mitotic chromosomes of rye and wheat resulting suitable for both their identification and detection of targeted sites.
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  • 59
    ISSN: 1573-5060
    Keywords: alien introduction ; chromosome pairing ; fluorescent in situ hybridization ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fluorescent in situ hybridization (FISH) of DNA to plant chromosomes has proved to be a powerful cytogenetic tool. The value of fluorescent in situ hybridization of total genomic DNA (GISH) of related species is demonstrated in the determination of wheat/alien chromosome pairing in hybrids. Its use for assessing the relative merits of the various genes that affect chromosome pairing is also shown. The ability of GISH to identify the presence in wheat of whole alien chromosomes or alien chromosome segments is illustrated. The potential of FISH for detecting repeated DNA sequences, low copy sequences and single copy genes is discussed.
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  • 60
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    Euphytica 89 (1996), S. 215-221 
    ISSN: 1573-5060
    Keywords: agronomic characteristics ; breeding trends ; quality ; year of release ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat breeding efforts for a half century in Japan were investigated by using of 129 varieties registered in MAFF from 1929 to 1984 in the view point of case history for three years. Varieties released in each breeding station are classified apparently by growth habit associated closely with duration of the cold requirement. Heading date is earlier in varieties released in southwest breeding stations (southern varieties) than in varieties released in northern breeding stations (northern varieties). Culm length is higher and the pre-harvest sprouting is more sensitive in northern varieties. In quality characters, northern varieties has the higher milling rate, lower apparent amylose content and larger particle size of flour. In varieties released in the Tohoku district, trend of breeding direction with time is smaller ear numbers and larger 1,000-grain weight and greater resistance to powdery mildew. In varieties released in the Kyushu district, a significant correlation with released year was clearly observed with regard to early maturity, short culm length, less grain crude protein content, less apparent amylose content and higher milling rate on breeding advancement.
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  • 61
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; stripe rust ; epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten spring bread wheat cultivars with a susceptible seedling reaction to race 14E14 of yellow rust were tested at three locations to assess the level and stability of quantitative resistance. Quantitative resistance was expressed in terms of disease severity (DS), area under the disease progress curve (AUDPC), apparent infection rate (r), infection type (IT) and infection density. Large genotypic differences were observed for all variables measured. Morocco was the most susceptible cultivar. Based on its high infection type (IT=9) throughout the epidemics, it most likely does not possess any resistance. All other cultivars carry quantitative resistance. The levels ranged from very low (Taichung 23) to very high (Parula). Resistance levels were lower in Quito, Ecuador than at the other locations. Most likely, the lower temperatures in Quito resulted in a reduced expression of quantitative resistance to yellow rust and to obtain the same protection as at the other two locations, more resistance genes are needed. Therefore, to accumulate genes for quantitative resistance, Quito is considered to be the better location. Though significant cultivar-location interactions were detected, they were small compared to the cultivar and location effect. Therefore, they are considered of little importance and it is concluded that quantitative resistance is a stable trait, in the sense that cultivar rankings are hardly affected by environment. The contribution of infection growth to the development of yellow rust was demonstrated. Between 29 and 66% of the increase in disease severity could be contributed to growth of infections. These figures are probably an underestimation of the real contribution as new infections are very small, thus reducing the average size of infections and their contribution to the increase of disease severity.
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  • 62
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    Euphytica 91 (1996), S. 31-35 
    ISSN: 1573-5060
    Keywords: aluminum ; linkage map ; marker ; RFLP mapping ; Triticum aestivum ; Triticum turgidum ; wheat ; durum wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The tolerance of aluminum (Al) of disomic substitution lines having the chromosomes of the D genome of Triticum aestivum L. cv. Chinese Spring individually substituted for their homoeologues in T. turgidum L. cv. Langdon was investigated by the hematoxylin method. The disomic substitution lines involving chromosome 4D were more Al tolerant than Langdon. The tolerance was found to be controlled by a single dominant gene, designated Alt2, that is in the proximal region of the long arm of chromosome 4D. The locus was mapped relative to molecular markers utilizing a population of recombinant chromosomes from homoeologous recombination between Chinese Spring chromosome 4D and T. turgidum chromosome 4B. Comparison of the location of Alt2 in this map with a consensus map of chromosomes 4B and 4D based on homologous recombination indicated that Alt2 is in a vicinity of a 4 cM interval delineated by markers Xpsr914 and Xpsr1051. The Alt2 locus is distal to marker Xpsr39 and proximal to XksuC2. The Altw locus is also proximal to the Knal locus on chromosome 4D that controls K+/Na+ selectivity and salt tolerance. In two lines, Alt 2 and Knal were transferred on a single 4D segment into the long arm of T. turgidum chromosome 4B.
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  • 63
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    Euphytica 91 (1996), S. 127-140 
    ISSN: 1573-5060
    Keywords: In vitro selection ; salt tolerance ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Embryogenic calli isolated from immature embryos of four wheat cultivars were subjected to three in vitro selection methods for salt tolerance. The effect of NaCl on the selected and unselected cell lines has been investigated. The results indicated that the relative growth rate of callus decreased as the concentration of NaCl increased in both callus lines. The selected callus line gave a higher growth weight in the presence of NaCl in the medium and was highly significant as compared with unselected callus line across medium protocols in all wheat cultivars. The dry weight of both kinds of callus lines of all wheat cultivars increased markedly with increasing NaCl concentration in most cases. The Na+ and Cl- contents of both callus lines were increased with increasing salinity levels while K+ content was decreased. The selected callus line of each cultivar at the same salinity level produced significant amounts of Na+, K+ and Cl- higher than the unselected callus line in most salinity levels. However, the unselected callus lines of the cultivars Giza-157 and Sakha-90 at the same salinity level produced significant amounts of K+ higher than the selected callus line in most salinity levels. The proline content of both kinds of callus lines for all wheat cultivars was increased with increasing salinity level. However, the selected callus line gave a significantly higher proline content than the unselected callus line in all wheat cultivars at the same Salinity level. Results from the in vitro selection for NaCl tolerance showed that the stepwise method of increasing NaCl in the medium was more effective for plant regeneration than other methods.
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  • 64
    ISSN: 1573-5060
    Keywords: adaptation ; intrinsic earliness ; Triticum aestivum ; vernalization ; wheat ; Syria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study sought to identify factors that influence wheat development in the transitional wheat growing zone of northern Syria. Three development factors were studied, intrinsic earliness, and responses to vernalization and to photoperiod. Two sets of wheat were studied, each composed of lines with differing combinations of development factors. Set 1 comprised 20 parental and breeding lines utilized by the CIMMYT/ICARDA facultative and winter wheat breeding program based at Tel Hadya. Set 2 comprised 19 parental and breeding lines utilized by an Australian winter wheat breeding program based at Temora. Field development was recorded in greatest detail at one site. Tel Hadya, using the state of differentiation of the apex of the main tiller of sampled plants. To extend findings, development was also recorded as the time from sowing to ear emergence for later sowings of wheat at Tel Hadya, and in sowings at four other regional sites. The significance of each development factor was tested in multiple regressions that predicted either stage of apical development at Tel Hadya, or time to ear emergence in all trials. It was found that intrinsic earliness was the major factor associated with development, in both sets of wheat. Response to photoperiod had a much smaller and less consistent effect. Response to vernalization had least effect on development, possibly because low temperature in winter delayed development for a longer period than was required to fully vernalize winter wheats. Our results suggested it may not be directly relevant whether spring or winter wheats are grown in the transitional zone of northern Syria. The desired phenotype for the region, of slow development prior to double ridge, then fast development to ear emergence, cannot be simply achieved from combinations of the three development factors. Selection for improved adaptation to the region must continue to rely on direct field observations.
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  • 65
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    Euphytica 92 (1996), S. 147-153 
    ISSN: 1573-5060
    Keywords: breeding methodology ; drought patterns ; input-efficiency ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary About 32% of the 99 million ha wheat grown in developing countries experiences varying levels of drought stress. Three major drought types have been identified: Late drought (LD) is common in the Mediterranean region, early drought (ED) is found in Latin America and wheat is produced on residual soil moisture (RM) in the Indian subcontinent and part of Australia. Until 1983, CIMMYT selected all germplasm under near optimum conditions for its yield potential and tested only advanced lines under drought. In spite of many critics, this approach proved to be successful, since in the mid 80's CIMMYT germplasm was grown on 45% of the wheat area in LC with annual rainfall from 300–500 mm and on 21% in areas with less than 300 mm. Since 1983, CIMMYT's drought breeding methodology is to alternate segregating populations between drought stressed and fully irrigated conditions (FI) and to test advanced lines under a line source irrigation system. To compare the efficiency of these approach, yield of four, mostly leading varieties, from each of the regions with LD, ED, RM, and FI and twelve recent CIMMYT cultivars selected for high yield under FI and RM conditions (ALT) were compared under four different moisture regimes (FI, LD, ED, and RM) in 89–90 and 90–91 in Yaqui Valley, Mexico. Genotypic correlation between yield and days to flowering, days to maturity, height, grains m-2, TKW, test weight and grain fill period were calculated. Mean grain yield of the four best lines in the ALT group was highest under all moisture stress regimes, followed by the FI-group. However, the highest yielding cultivar within each moisture regime was from the FI-group under FI, from the LD-group under LD, and from the ALT-group under ED and RM conditions. Estimates for genetic advance suggest that FI is the best environment for increasing grain yield even in all three drought environments. This indicates that yield potential per se is beneficial also in drought environments. The highest yield in drought environments was realized by the CIM cultivars selected under FI and RM. Simultaneous evaluation of the germplasm under near optimum conditions, to utilize high heritabilities and identify lines with high yield potential, and under stress conditions to preserve alleles for drought tolerance seem at present the best strategy.
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  • 66
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    Euphytica 92 (1996), S. 221-224 
    ISSN: 1573-5060
    Keywords: hybrid lines ; nitrogen efficiency ; variation ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The new European Common Agricultural Policy and environmental considerations are certainly to change agricultural practices toward low input cultivation systems. Nitrogen is one of the main inputs of winter wheat in northern France and it contributes highly to phreatic water pollution. A research programme has then been set up in order to study whether it is possible to breed for winter wheat cultivars using more efficiently N fertilisers. Less nitrogen would be applied, decreasing pollution risks and operational costs. It has been shown that a large variation exists for N related traits and for the resistance against N deficiency. On the one hand the cv Arche is very resistant to N deficiency, its yield on low N conditions (with no N fertiliser) is on average 89% of its yield on high N conditions (with a high N application). On the other hand, cv Récital is very susceptible to N deficiency as this same percentage is only 61%. A study on 10 hybrids showed that heterosis for grain yield was higher at low N level than at high N level. This was due to a higher number of grains per m2.
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  • 67
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    Plant and soil 178 (1996), S. 223-230 
    ISSN: 1573-5036
    Keywords: barley ; cultivar ; acquisition efficiency ; rhizosphere ; root hairs ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To assess the extent of variation in phosphorus acquisition efficiency of some winter wheat (Triticum aestivum L.), winter and spring barley (Hordeum vulgare L.) genotypes, depletion of inorganic phosphorus (P) extractable with 0.5 M NaHCO3 (NaHCO3-Pi) from the rhizosphere soil was studied. Nutrients supply, rhizosphere soil pH and soil water content was kept equal for all the genotypes with the aim to reduce the confounding variation due to these factors. The experimental set up implied that no difference in the relative growth rates, nitrogen, potassium and calcium content of shoot dry matter occurred among the genotypes. The winter wheat, winter barley and spring barley genotypes differed significantly (p〉0.05) in their efficiency to acquire NaHCO3-Pi from the rhizosphere soil. The efficiency of the winter wheat genotypes to acquire NaHCO3-Pi from rhizosphere soil ranked Kraka 〉 Gawain 〉 Foreman 〉 Sleipner = Obelisk 〉 Kosack 〉 Pepital 〉 Arum. Winter wheat genotypes differed in extent of P depletion profiles in the rhizosphere, indicating variation in root hair length. The winter barley and spring barley genotypes also showed significant differences in their P depletion profiles near roots. The efficiency of the winter barley genotypes to acquire soil P in the rhizosphere ranked Hamu 〉 Frost 〉 Marinka 〉 Astrid 〉 Clarine = Angora. The efficiency of spring barley genotypes to acquire NaHCO3-Pi in the rhizosphere ranked Canut 〉 Etna ≅ Riga 〉 Digger 〉 Peel 〉 Semal 〉 Alexis. The rhizosphere pH remained unchanged, suggesting that additional mechanisms such as root hair formation and root exudates play a significant role in causing variation in P acquisition among the genotypes.
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  • 68
    ISSN: 1573-5036
    Keywords: diffusion ; models ; nitrogen ; nutrient uptake ; phosphorus process-oriented ; resource use ; rooted volume ; rooting density ; root parameters ; roots ; spatial distribution ; uptake models ; water uptake ; zero sink
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A review is given of the prospects for using process-oriented models of water and nutrient uptake in improving ‘integrated agriculture’. Government-imposed restrictions on the use of external inputs will increase the likelihood of (temporary) nutrient or water stress in crop production in NW Europe and thus a better understanding is required of shoot-root-soil interactions than presently available. In modelling nutrient and water uptake, three approaches are possible: 1) models-without-roots, based on empirically derived efficiency ratios for uptake of available resources, 2) models evaluating the uptake potential of root systems as actually found in the field and 3) models which also aim at a prediction of root development as influenced by interactions with environmental factors. For the second type of models the major underlying processes are known and research can concentrate on model refinement on the one hand and practical application on the other. The main parameters required for such models are discussed and examples are given of practical applications. For the third type of models quantification of processes known only qualitatively is urgently needed.
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  • 69
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    Plant and soil 186 (1996), S. 39-43 
    ISSN: 1573-5036
    Keywords: acetylene reduction ; drought ; drought-avoidant germplasm ; Glycine max (L.) Merrill ; growth ; nitrogen ; assimilation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Inoculated soybeans (Glycine max L. (Merrill)) were grown in controlled environments to evaluate the relationship between genotype and plant water status on nodule function, nitrogen assimilation, growth rates, and seed yield. Plants were grown under well-watered (WW) and water-stressed (WS) conditions during the linear pod-filling growth stage in sand culture using N-free nutrient solution. Dry matter and N accumulation were greater for the drought-adapted Plant Introduction 416937 (PI) than for ‘Forrest’, a commercially adapted genotype of similar phenology. These differences are attributed to: (i) more favorable internal water balance throughout the pod-filling period (higher total leaf water potential), (ii) higher photosynthetic function (more total leaf area and higher net carbon exchange rates), and (iii) stronger nodule function (larger nodule mass, greater specific and total nodule activity, and thus more nitrogen assimilation) for the PI than for Forrest. While Forrest out yielded the PI under WW conditions, the percentage reduction in seed mass per plant was less for the PI than for Forrest when both genotypes were exposed to desiccating conditions. The inference is that soybean germplasm with the capacity to maintain tissue turgidity, and thus leaf and nodule function, during reproductively-imposed desiccation may reduce the extent to which yield is compromised during drought. These findings have implications for the role of symbiotic nitrogen fixation in conserving yield under dry weather conditions.
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  • 70
    ISSN: 1573-5036
    Keywords: amino acids ; arginine ; fertilization ; mineral nutrients ; nitrogen ; nitrogen deposition ; Pinus sylvestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aim of the investigation was to study if improved nutrient status in Scots pine (Pinus sylvestris L) trees would be reflected in decreased concentrations of arginine in the needles. The studies trees had imbalanced mineral nutrient composition and elevated needle arginine concentrations caused by long-term fertilization with N. Concentrations of arginine and mineral nutrients in needles were followed over three consecutive years of additional fertilization with N alone or with P, K, Mg and micronutrients in combination with and without N. Analysis of needle mineral concentrations suggested that there were deficiencies only in K and Mg. The N concentration increased both in trees fertilized with N alone and in trees fertilized with N in combination with mineral nutrients. In the control treatment and in trees fertilized with mineral nutrients other than N the N concentration remained fairly constant. The highest Ca/N, K/N and P/N ratios were found in trees fertilized with mineral nutrients other than N while the lowest ratios were found in trees fertilized with N alone. Arginine concentrations in needles from trees fertilized with N alone remained at a high level throughout the experiment while arginine concentrations in trees given the other treatments decreased. The results show that the mineral nutrient balance can be improved with appropriate fertilization and that this improvement is reflected in decreasing arginine levels. Furthermore the study demonstrates that when N supply is reduced the arginine concentration also decreases also as an effect of reduced N supply per se. The study also indicates that arginine may be a better measure of the N status in pine trees than total N.
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  • 71
    ISSN: 1573-5036
    Keywords: manure ; mineralization ; modelling ; nitrogen ; organic matter ; slurry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A computer simulation model of the turnover of organic matter in soil was adapted to simulate the change in soil organic C and N contents of soil during several years following annual additions of farm slurry to maize fields. The model proved successful in estimating the build-up of both C and N in soil and the leaching of N to ground-water in response to applications of slurry ranging from 50 to 300 tons per hectare per year. The model was then used to estimate the build-up of organic matter in soil under crops of fodder maize that were grown using the excess of manure produced during the last 20 years in the Netherlands. The build-up of organic matter from these applications was estimated to lead to about 70 kg extra nitrogen mineralized ha-1 yr-1. As a result of legislation manure applications have decreased and are expected to decrease further in the immediate future. Calculations suggest that after 10 years of manure applied at rates no longer exceeding the amount needed to replace the phosphorus removed by crops, the extra mineralization of N will still be between 45 and 60 kg ha-1 yr-1. If manure applications cease altogether then the extra mineralization will be about 25–30 kg N ha-1 yr-1.
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  • 72
    ISSN: 1573-5036
    Keywords: foliar nutrient contents ; nitrogen ; Picea abies ; root growth ; root morphology ; root/shoot ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract During a seven-month period the effect of different nitrogen (N) availability in soil on growth and nutrient uptake was studied in three-year-old Norway spruce (Picea abies [L.] Karst.) trees. The plants were grown in pots on N-poor forest soil supplied with various amounts and forms (inorganic and organic) of N. Increasing supply of inorganic N (as NH4NO3) increased the formation of new shoots and shoot dry weight. The root/shoot dry weight ratio of new growth was drastically decreased from 1.6 in plants without N supply to 0.5 in plants supplied with high levels of NH4NO3. This decrease in root/shoot dry weight ratio was associated with distinct changes in root morphology in favour of shorter and thicker roots. The addition of keratin as organic N source did neither affect growth nor root morphology of the trees. The amount of N taken up by plants was closely related to the supply of inorganic N, and trees supplied with highest levels of NH4NO3 also had the highest N contents in the dry matter of needles and roots. In contrast, N contents in needles of trees grown without additional N, or with keratin supply, were in the deficiency range. Supply of NH4NO3 decreased the contents of phosphate (P) and potassium (K) and therefore markedly increased N/P and N/K ratios in the needles. On the other hand, the contents of calcium (Ca), magnesium (Mg), and manganese (Mn) in the needles were increased in the plants supplied with inorganic N, suggesting high soil availability and promotion of uptake of these divalent cations by high nitrate uptake. The observed effects on root/shoot dry weight ratio, root morphology, and mineral nutrient composition of the needles indicated that high inorganic N supply may increase above-ground productivity but at the same time decrease the tolerance of trees against soil-borne (e.g. deficiency of other mineral nutrients) stress factors.
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  • 73
    ISSN: 1573-5036
    Keywords: acetylene inhibition ; calcium carbide ; denitrification ; nitrogen ; vegetable production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The use of calcium carbide (CaC2) pellets as a source of acetylene (C2H2) for the C2H2 inhibition technique to measure denitrification in the field was examined in a sandy loam soil cropped to lettuce. CaC2 was used in combination with vented closed chambers. Three grams of 2–4 mm CaC2 pellets were inserted at 7 and 20 cm for raised beds, and 6 and 15 cm for furrows, in four holes located at right angles, 2.5 cm outside a 20 cm dia. chamber pushed 4 cm into the soil. Acetylene concentrations were determined in the centre of the chamber 1.0 and 4.0 hours after applying CaC2, in beds (two dates) and furrows (one date) at different depths to 20 and 15 cm, respectively, for beds and furrows. At soil matric potentials of ca. 0.01 and ca. 0.06 MPa, soil C2H2 concentrations were 〉1.0% (v/v) for all determinations. Weekly measurements of denitrification were made from a sprinkler irrigated lettuce crop for 8 weeks. Measurements were made 24 hours after irrigation and on one occasion 48 hours after. Measured mean rates of denitrification were generally 〈100 g N ha−1 d−1, much less than published data following furrow irrigation of vegetable crops in a nearby region. In another sandy loam soil at ca. 0.08 MPa, soil C2H2 concentrations were ≥2.0 % at 5 and 15 cm, 1.0 and 6.0 hours after adding CaC2. Using CaC2 to generate C2H2 in soil is a convenient approach for field measurements where the C2H2 inhibition technique is used with chambers to determine the nitrous oxide flux. In comparison with C2H2 supply via probe systems, CaC2 overcomes the requirements for cylinders of pressurised C2H2 gas and complex on-site plumbing.
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  • 74
    ISSN: 1573-5036
    Keywords: deprivation ; intermitten ; nitrogen ; roses ; uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A recirculating nutrient solution system was utilized to study the effect of intermittent N deprivation on N uptake by mature ‘Royalty’ rose over the course of one flowering cycle. Plants received a nutrient solution lacking N for 4, 8 or 16 days, after which one containing NO3−N (0.75 mM) was supplied for 4 days. N-deprivation resulted in a 2–3 fold increase in N uptake rate compared to control plants supplied continuously with N. The magnitude of this deprivation-enhanced N uptake was not affected by either the duration of N-deprivation or the plant developmental stage. Over the course of the flowering cycle, the total cumulative N uptake by the plants was 95, 66, and 44% of the control plants in the 4, 8 and 16-day deprivation treatments, respectively. A characteristic diurnal pattern of N uptake occurred in both N-starved and control plants. Uptake oscillated between minimum rates in the morning and maximum rates in the evening, the latter occurring 4–6 hr after the maximum rate of transpiration.
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  • 75
    ISSN: 1573-5036
    Keywords: genotypes ; phytosiderophores ; wheat ; zinc deficiency ; zinc efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of the zinc (Zn) nutritional status on the rate of phytosiderophore release was studied in nutrient solution over 20 days in four bread wheat (Triticum aestivum cvs. Kiraç-66, Gerek-79, Aroona and Kirkpinar) and four durum wheat (Triticum durum cvs. BDMM-19, Kunduru-1149, Kiziltan-91 and Durati) genotypes differing in Zn efficiency. Visual Zn deficiency symptoms, such as whitish-brown necrosis on leaves and reduction in plant height appeared first and more severe in Zn-inefficient durum wheat genotypes Kiziltan-91, Durati and Kunduru-1149. Compared to the bread wheat genotypes, all durum wheat genotypes were more sensitive to Zn deficiency. BDMM-19 was the least affected durum wheat genotype. Among the bread wheat genotypes, Kirkpinar was the most sensitive genotype. In all genotypes well supplied with Zn, the rate of phytosiderophore release was very low and did not exceed 1 μmol 32 plants-1 3h-1, or 0.5 μmol g-1 root dry wt 3h-1. However, under Zn deficiency, with the onset of visual Zn deficiency symptoms, the release of phytosiderophores was enhanced in bread wheat genotypes up to 7.5 μmol 32 plants-1 3h-1, or 9 μmol g-1 root dry wt 3h-1, particularly in Zn-efficient Kiraç-66, Gerek-79 and Aroona. In contrast to bread wheat genotypes, phytosiderophore release in Zn-deficient durum wheat genotypes remained at a very low rate. Among the durum wheat genotypes BDMM-19 had highest rate of phytosiderophore release. HPLC analysis of root exudates showed that 2′-deoxymugineic acid (DMA) is the dominating phytosiderophore released from roots of Zn-efficient genotypes. In root extracts concentration of DMA was also much higher in Zn-efficient than in inefficient genotypes. The results demonstrate that enhanced synthesis and release of phytosiderophores at deficient Zn supply is involved in Zn efficiency in wheat genotypes. It is suggested that the expression of Zn efficiency mechanism is causally related to phytosiderophore-mediated enhanced mobilization of Zn from sparingly soluble Zn pools and from adsorption sites, both in the rhizosphere and plants.
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  • 76
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    Plant and soil 181 (1996), S. 1-6 
    ISSN: 1573-5036
    Keywords: leaching ; nitrate ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In studies of nitrate leaching both experimenters and modellers experience problems arising from soil variability. Because of the small-scale heterogeneity that gives rise to mobile and immobile categories of water, both measurements and modelling are easiest in homogeneous sandy soils and most difficult in strongly structured clay soils. There are also parallels at plot and field scale in the problems caused to experimenters by log-normal distributions of nitrate concentrations and those caused to modellers by non-linearity in models. All researchers need to be aware that a reliable estimate of the mean from a set of measurements or a model may necessitate considerations of variances as well as means.
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  • 77
    ISSN: 1573-5036
    Keywords: ammonia volatilization ; denitrification ; grazed pasture ; leaching ; nitrogen ; N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Inputs and losses of nitrogen (N) were determined in dairy cow farmlets receiving 0, 225 or 360 kg N ha-1 (in split applications as urea) in the first year of a large grazing experiment near Hamilton, New Zealand. Cows grazed perennial ryegrass/white clover pastures all year round on a free-draining soil. N2 fixation was estimated (using 15N dilution) to be 212, 165 and 74 kg N ha-1 yr-1 in the 0, 225 and 360 N treatments, respectively. The intermediate N rate had little effect on clover growth during spring but favoured more total pasture cover in summer and autumn, thereby reducing overgrazing and resulting in 140% more clover growth during the latter period. Removal of N in milk was 76,89 and 92 kg N ha-1 in the 0, 225 and 360 N treatments, respectively. Denitrification losses were low (7–14 kg N ha-1 yr-1), increased with N application, and occurred predominantly during winter. Ammonia volatilization was estimated by micrometeorological mass balance at 15, 45 and 63 kg N ha-1 yr-1 in the 0, 225 and 360 N treatments, respectively. Most of the increase in ammonia loss was attributed to direct loss after application of the urea fertilizer. Leaching of nitrate was estimated (using ceramic cup samplers at 1 m soil depth, in conjunction with lysimeters) to be 13, 18 and 31 kg N ha-1 yr-1 in a year of relatively low rainfall (990 mm yr-1) and drainage (170–210 mm yr-1). Drainage was lower in the N fertilized treatments and this was attributed to enhanced evapotranspiration associated with increased grass growth. Nitrate-N concentrations in leachates increased gradually over time to 30 mg L-1 in the 360 N treatment whereas there was little temporal variation evident in the 0 (mean 6.4 mg L-1) and 225 (mean 10.1 mg L-1) N treatments. Thus, the 360 N treatment had a major effect by greatly reducing N2 fixation and increasing N losses, whereas the 225 N treatment had little effect on N2 fixation or on nitrate leaching. However, these results refer to the first year of the experiment and further measurements over time will determine the longer-term effects of these treatments on N inputs, transformations and losses.
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  • 78
    ISSN: 1573-5036
    Keywords: Barro Colorado Island ; base cations ; nitrogen ; phosphorus ; seasonal variation ; semideciduous forest ; soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of seasonal water availability on soil nutrients and soil N transformations was investigated by irrigating two large plots of mature tropical forest on Barro Colorado Island (BCI), Panama, during the dry season for five consecutive years. Methods included (i) nutrient accumulation by ion-exchange resins placed on the surface of the mineral soil for contiguous 21-day periods, (ii) monthly mineral soil (0–10 cm) extractions and incubations for inorganic N and P concentrations, and (iii) leaching loss of nutrients from leaf litter samples. Rates of nutrient accumulation by the resins showed a great deal of variation between sampling dates and among years in control plots; albeit, seasonal patterns were slight, except for the highest Ca values near the end of the wet season and inorganic P (Pi) and SO4 values that peaked during the dry season. Irrigation had remarkably little effect on nutrient accumulation rates by resins, except for an increase in Mg and Na values, but did affect the timing in the temporal variation in K, Na, Ni and Pi values. In contrast, inorganic N (Ni) and Pi pools and N transformation rates in the mineral soil hardly varied among sampling dates and did not show any response to irrigation. We hypothesize that the timing of leaf litterfall and nutrient leaching from forest floor litter can set up temporal patterns in the levels of soil nutrient at the surface of the mineral soil, but the temporal patterns essentially disappear with depth in the mineral soil.
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  • 79
    ISSN: 1573-5036
    Keywords: ammonia volatilization ; animal manure ; N immobilization ; N mineralization ; N-balance ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The fate of nitrogen from 15N-labelled sheep urine and urea applied to two soils was studied under field conditions. Labelled and stored urine equivalent to 204 kg N ha−1 was either incorporated in soil or applied to the soil surface prior to sowing of Italian ryegrass (Lolium multiflorum L.), or it was applied to ryegrass one month after sowing. In a sandy loam soil, 62% of the incorporated urine N and 78% of the incorporated urea N was recovered in three cuts of herbage after 5 months. In a sandy soil, 51–53% of the labelled N was recovered in the herbage and the distribution of labelled N in plant and soil was not significantly different for incorporated urine and urea. Almost all the supplied labelled N was accounted for in soil and herbage in the sandy loam soil, whereas 33–34% of the labelled N was unaccounted for in the sandy soil. When the stored urine was applied to the soil surface, 20–24% less labelled N was recovered in herbage plus soil compared to the treatments where urine or urea were incorporated, irrespective of soil type. After a simulated urination on grass, 69% of the labelled urine N was recovered in herbage and 15% of the labelled N was unaccounted for. The labelled N unaccounted for was probably mainly lost by ammonia volatilization. Significantly more urine- than urea-derived N (36 and 19%, respectively) was immobilized in the sandy loam soil, whereas the immobilization of N from urea and urine was similar in the sandy soil (13–16%). The distribution of urine N, whether incorporated or applied to the soil surface prior to sowing, did not influence the immobilization of labelled urine N in soil. The immobilization of urine-derived N was also similar whether the urine was applied alone or in an animal slurry consisting of labelled urine and unlabelled faecal N. When urine was applied to growing ryegrass at the sandy loam soil, the immobilization of urine-derived N was significantly reduced compared to application prior to sowing. The results indicated that the net mineralization of urine N was similar to that of urea in the sandy soil, but only about 75% of the urine N was net mineralized in the sandy loam soil, when urine was applied prior to sowing. Thus, the fertilizer effect of urine N may be significantly lower than that of urea N on fine-textured soils, even when gaseous losses of urine N are negligible.
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  • 80
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; gene action ; heritability ; wheat ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Estimates of gene actions were obtained for five in vitro traits of immature wheat (Triticum aestivum L.) embryo cultures from a cross of two wheat cultivars and the resulting reciprocal, F1, F2 and backcross populations. The contribution of additive gene effects to in vitro traits was not as important as the dominance gene effects. Epistatic gene effects were relatively more important than either additive or dominance gene effects. Of the individual types of digenic epistatic effects, the dominance x dominance estimates were relatively larger in magnitude for all in vitro culture traits measured. The maternal effect played a minor role in the inheritance of the in vitro studied traits since the difference among the reciprocal values was not significant. It is shown from the generation mean method that epistasis played a major role in the inheritance of most of the traits under study. The negative values of additive and dominance genetic variance were estimates of zero. Heritability estimates, in broad sense, were relatively high for the in vitro studied traits. In some cases, heritability estimates in broad and narrow senses are almost equal since the estimation of dominance genetic variance led to negative values. According to the results of the gene effects, dominance and epistasis were important for the shoot formation trait. Selection would be effective among the isolated genotypes on individual basis.
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  • 81
    ISSN: 1573-5060
    Keywords: Secale cereale ; Triticum aestivum ; yield components ; 1AL/1RS translocation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of the 1AL/1RS chromosome translocation on grain yield and other agronomic characteristics of 85 random F2-derived F6 bulks from three 1AL 1RS × 1A bread wheat crosses was determined under optimum and reduced irrigation conditions at CIANO, Yaqui Valley, Sonora, Mexico, during the 1991–1992 and 1992–1993 crop production cycles. Harvest plots of 5.0 m2 were arranged in an alpha lattice design with three replications. The 1AL/1RS translocation increased grain yield, above-ground biomass, spikes/m2, and test weight under both irrigated and dryland conditions. Homozygous chromosome 1A lines, on the other hand, possessed longer spikes with more grains. The 1AL/1RS cultivars had an advantage in 1000-grain weight, which was detected only under optimum irrigation. The translocation lines showed later maturity and longer grainfilling period than the 1A genotypes under one irrigation treatment. A significant relationship between grain yield and test weight was detected only among the 1AL/1RS genotypes, indicating that they possess heavier and plumper grains than the 1A genotypes. These results encourage the continued use of the 1AL/1RS translocation in wheat improvement.
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  • 82
    ISSN: 1573-5060
    Keywords: embryogenesis ; wheat ; maize ; Triticum aestivum ; Zea mays ; haploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Embryogenesis was analyzed in wheat × maize hybrids using paraffin sectioning. Embryogenesis in wheat × maize hybrids is different from that in self-pollinated wheat plants. Development of the embryo is not accompanied by the formation of an endosperm. The endosperm nuclei remain free in the cytoplasm, fail to advance into the cellular stage, and degenerate at a later time. The antipodal cells quickly degenerate in the fertilized ovaries of wheat × maize hybrids similar to self-pollinated ovaries. The antipodal cells remain normal in unpollinated ovaries. The pre-embryo will abort if it is allowed to develop on the plant, because of a nutritional shortage in the absence of an endosperm. Therefore, embryo rescue is necessary for haploid production from a wheat × maize hybrids. Haploid polyembryos were obtained from spikelet culture of wheat × maize hybrids. The formation of polyembryos is due to the cleavage of the pre-embryo and the effect of 2,4-D. The frequency of haploid embryo production and plant regeneration is affected significantly by maize genotypes, but not by wheat genotypes. The concentration of 2,4-D affects only the size of the embryo.
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  • 83
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    Euphytica 92 (1996), S. 95-103 
    ISSN: 1573-5060
    Keywords: rusts ; virulence genes ; wheat ; resistance genes ; Czech Republic ; Slovak Republic ; Triticum aestivum ; Thalictrum species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In former Czechoslovakia virulence of rusts attacking wheat was studied since the sixties. Since the same time genes for resistance in the registered cultivars were identified. The role of Berberis and Thalictrum as alternate hosts for stem rust and leaf rust, respectively, was investigated as well. Determined changes of virulence in rust populations could only partially be ascribed to changes of resistance genes in the grown cultivars. Unnecessary genes for virulence had no negative effect on the fitness of the pathogen. All tested samples of aeciospores from barberries attacked rye, not wheat. None of Thalictrum species occurring in the Czech and Slovak Republics was found to host wheat leaf rust. However, the sexual stage of wheat stem rust and wheat leaf rust could be induced on Berberis vulgaris and Thalictrum speciosissimum, respectively. General epidemiological conclusions are drawn from the results and experience of the last 35 years.
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  • 84
    ISSN: 1573-5109
    Keywords: genetic diversity ; HMW glutenin subunits ; landraces ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sixty hexaploid wheat landraces collected from five regions of Pakistan were assessed for genetic variability in terms of high molecular weight (HMW) glutenin subunits as revealed by SDS-PAGE. The germplasm appeared to be diverse and unique on the basis of HMW glutenin subunit compositions. Out of 24 alleles detected at all the Glu-1 loci, four belonged to Glu-A1, 12 to Glu-B1 and eight to Glu-D1 locus. The number of novel HMW glutenin subunits detected were 1, 4 and 6 at the three loci (Glu-A1, Glu-B1, Glu-D1), respectively. The frequency distribution patterns of 24 allelic variants detected at the three Glu-1 loci in 1080 samples analysed for 60 accessions were determined both on the basis of individual accessions and on the basis of regions (accessions pooled across the regions). One allele (“null”) at the Glu-A1 locus, three alleles (17+18, 7+8, 14) at the Glu-B1 locus and, two alleles (2+12 and 2**+12′) at the Glu-D1 locus were found most frequently distributed in the 60 populations. Maximum variation was observed in the Baluchistan and Gilgit regions of Pakistan in terms of distribution of novel Glu-1 alleles. A higher gene diversity was observed between the populations as compared to the gene diversity within the populations while, a reverse pattern of gene diversity was observed when populations were pooled across the regions (higher within the regions than between the regions). A data base has been generated in this study which could be expanded and usefully exploited for cultivar development or management of gene bank accessions.
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  • 85
    ISSN: 1573-5117
    Keywords: reservoir ; phosphate ; nitrogen ; sedimentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to study the N and P balances in the Driss I reservoir, we measured concentrations in the water of these elements, their sedimentation rates, and their input by the river Inaouen and output through the dam. Supply and loss of N and P were calculated from samples collected every 48 hours and were for N 15 and 27. 5 mg m−2 d−1 and for P 33.6 and 1.1 mg m−2 d−1 respectively. The sedimentation rates, determined using sediment traps, were high, about 75 mg m−2 d−1 for N and 34 mg m−2 d−1 for P. Phosphate input came from point sources and was mainly in particulate form. A large fraction of the phosphate sedimented in the form of calcium bound phosphate, with some iron bound phosphate. Bioavailability was, however, low, as the particulate phosphate disappeared rapidly from the epilimnion because of the high sedimentation rate. The ratio Tot-N/Tot-P of the sedimented particulate matter varied between 0.05 and 7.74 and depended on primary production and watergate management.
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  • 86
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    Hydrobiologia 335 (1996), S. 159-170 
    ISSN: 1573-5117
    Keywords: denitrification ; mineral ; nitrogen ; Adour ; mineralisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A section of the River Adour (SW France) has been investigated to determine the role of the riparian vegetative strips (rvs) in the retention of mineral nitrogen. Entering and exiting fluxes have been measured along this segment throughout a hydrological season. The study indicates gains in mineral nitrogen (∼ 28%) during periods of low flow in the summer months, with riparian vegetation utilising 2.3 to 3.7% of the entering flux. Significant losses (∼ 50%) of nitrogen are observed in autumn, whereas minor losses are observed during the two flood events of November 1991 and June 1992 (4.6 – 4.9% of the entering flux). In winter losses of mineral nitrogen due to denitrification are small varying from 0.07% in winter, to 7% in summer, of the entering flux. The input of dissolved nitrogen through rainfall is estimated to be negligible within the zone studied. Additions of fertiliser for maize cultivation in February, May, June and July of 1992 have a significant impact on the fluxes exiting, the study zone. In May, the added nitrogen is used largely in the growth of the maize, what remains is is retained in the riparian vegetation. By contrast, in June, 27.2% of the available nitrogen added as fertiliser is found in the river. Finally, during the course of the hydrological year an increase in mineral nitrogen of 519 tonnes is observed in the area studied between Pontonx and Dax, of which only 224.8 tonnes can be ascribed to the addition of fertiliser. The remainder of this enrichment is explained by mineralisation of organic nitrogen stored in the riparian vegetative strips.
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  • 87
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    Biogeochemistry 32 (1996), S. 93-113 
    ISSN: 1573-515X
    Keywords: alpine ; biogeochemistry ; nitrogen ; nitrogen saturation ; snowmelt ; soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Experiments were conducted during 1993 at Niwot Ridge in the Colorado Front Range to determine if the insulating effect of winter snow cover allows soil microbial activity to significantly affect nitrogen inputs and outputs in alpine systems. Soil surface temperatures under seasonal snowpacks warmed from −14 °C in January to 0 °C by May 4th. Snowmelt began in mid-May and the sites were snow free by mid June. Heterotrophic microbial activity in snow-covered soils, measured as C02 production, was first identified on March 4, 1993. Net C02 flux increased from 55 mg CO2-C m−2 day−1 in early March to greater than 824 mg CO2-C m-2 day−1 by the middle of May. Carbon dioxide production decreased in late May as soils became saturated during snowmelt. Soil inorganic N concentrations increased before snowmelt, peaking between 101 and 276 mg kg−1 soil in May, and then decreasing as soils became saturated with melt water. Net N mineralization for the period of March 3 to May 4 ranged from 2.23 to 6.63 g N m−2, and were approximately two orders of magnitude greater than snowmelt inputs of 50.4 mg N m−2 for NH4 + and 97.2 mg N m−2 for NO3 −. Both NO3 − and NH4 + concentrations remained at or below detection limits in surface water during snowmelt, indicating the only export of inorganic N from the system was through gaseous losses. Nitrous oxide production under snow was first observed in early April. Production increased as soils warned, peaking at 75 μg N2O-N m−2 day−1 in soils saturated with melt water one week before the sites were snow free. These data suggest that microbial activity in snow-covered soils may play a key role in alpine N cycling before plants become active.
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  • 88
    ISSN: 1573-515X
    Keywords: lacustrine sediment ; organic matter ; sedimentation rates ; recycling ; burial ; carbon ; nitrogen ; silicon ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The areal distribution of organic C contents, δ13C values, total N and P and biogenic Si contents in surficial sediments were used to study the distribution, origin and diagenetic transformations of sedimented biogenic debris in the eutrophic subalpine Lake Bled (Slovenia), which for most of the yearhas an anoxic hypolimnion. The influence of an allochthonous input, restricted to the western basin, was clearly traced by higher organic C and total N and P contents, higher δ13C values, and higher sedimentation rate in comparison to the eastern basin. The low δ13C values of sedimentary organic matter in the major part of the lake, lower than the δ13C values of different types of organic matter, suggest that this sedimentary organic matter is most probably the product of a microbial community and not a residue of primary production. The temporal variation of benthic diffusive fluxes of NH4, Si and PO4, derived from modelling the pore water profiles, was related to sedimentation of phytoplanktonic blooms, while the PO4 fluxes were also dependent on changing redox conditions at the sediment-water interface in the period of the winter-spring overtum. The removal of PO4 in pore waters is probably due to the adsorption of phosphate and precipitation of apatite and vivianite. The budget of C, N and P at the sediment-water interface revealed a high recycling efficiency (〉70%), also confirmed by the rather uniform (or only slightly decreasing) vertical profiles of organic C, total N and P in sediment cores and C/N and C/P ratios. The percentage of biogenic Si recycling is low (〈10%), suggesting its removal in sediments.
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  • 89
    ISSN: 1573-515X
    Keywords: continental shelf ; estuaries ; mass balance ; nitrogen ; North Atlantic ; nutrient budget ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Five large rivers that discharge on the western North Atlantic continental shelf carry about 45% of the nitrogen (N) and 70% of the phosphorus (P) that others estimate to be the total flux of these elements from the entire North Atlantic watershed, including North, Central and South America, Europe, and Northwest Africa. We estimate that 61 · 109 moles y−1 of N and 20 · 109 moles y−1 of P from the large rivers are buried with sediments in their deltas, and that an equal amount of N and P from the large rivers is lost to the shelf through burial of river sediments that are deposited directly on the continental slope. The effective transport of active N and P from land to the shelf through the very large rivers is thus reduced to 292 · 109 moles y−1 of N and 13 · 109 moles y−1 of P. The remaining riverine fluxes from land must pass through estuaries. An analysis of annual total N and total P budgets for various estuaries around the North Atlantic revealed that the net fractional transport of these nutrients through estuaries to the continental shelf is inversely correlated with the log mean residence time of water in the system. This is consistent with numerous observations of nutrient retention and loss in temperate lakes. Denitrification is the major process responsible for removing N in most estuaries, and the fraction of total N input that is denitrified appears to be directly proportional to the log mean water residence time. In general, we estimate that estuarine processes retain and remove 30–65% of the total N and 10–55% of the total P that would otherwise pass into the coastal ocean. The resulting transport through estuaries to the shelf amounts to 172–335 · 109 moles y−1 of N and 11–19 · 109 moles y−1 of P. These values are similar to the effective contribution from the large rivers that discharge directly on the shelf. For the North Atlantic shelf as a whole, N fluxes from major rivers and estuaries exceed atmospheric deposition by a factor of 3.5–4.7, but this varies widely among regions of the shelf. For example, on the U.S. Atlantic shelf and on the northwest European shelf, atmospheric deposition of N may exceed estuarine exports. Denitrification in shelf sediments exceeds the combined N input from land and atmosphere by a factor of 1.4–2.2. This deficit must be met by a flux of N from the deeper ocean. Burial of organic matter fixed on the shelf removes only a small fraction of the total N and P input (2–12% of N from land and atmosphere; 1–17% of P), but it may be a significant loss for P in the North Sea and some other regions. The removal of N and P in fisheries landings is very small. The gross exchange of N and P between the shelf and the open ocean is much larger than inputs from land and, for the North Atlantic shelf as a whole, it may be much larger than the N and P removed through denitrification, burial, and fisheries. Overall, the North Atlantic continental shelf appears to remove some 700–950· 109 moles of N each year from the deep ocean and to transport somewhere between 18 and 30 · 109 moles of P to the open sea. If the N and P associated with riverine sediments deposited on the continental slope are included in the total balance, the net flux of N to the shelf is reduced by 60 · 109 moles y−1 and the P flux to the ocean is increased by 20 · 109 moles y−1. These conclusions are quite tentative, however, because of large uncertainties in our estimates of some important terms in the shelf mass balance.
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  • 90
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    Genetica 97 (1996), S. 243-254 
    ISSN: 1573-6857
    Keywords: durable resistance ; induced resistance ; leaf rust ; rust resistance ; stem rust ; stripe rust ; wheat ; wheat breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It is known that few wheat cultivars maintain their resistance to rust diseases for a long period of time, particularly when crop populations become genetically more uniform. A number of genetically diverse, so far unexploited, sources of rust resistance in the natural as well as mutagenized population of wheat cultivars were identified. Several of these genes were placed in agronomically superior well-adapted backgrounds so that they could be used as pre-breeding stocks for introducing genetic diversity for resistance in a crop population. Some of these stocks when employed as parents in several cross combinations in a breeding programme have generated a number of promising cultivars with diversity for resistance. Many presently grown wheats in India, near-isogenic lines each with Lr14b, Lr14ab, Lr30 and certain international cultivars were identified as possessing diverse sources of adult plant resistance (APR) to leaf rust. Prolonged leaf rust resistance in some of the Indian cultivars was attributed to the likely presence of Lr34 either alone or in combination with other APR components. Tests of allelism carried out in certain cultivars that continue to show adequate levels of field resistance confirm the presence of Lr34, which explains the role that this gene has played in imparting durability for resistance to leaf rust. Also, Lr34 in combination with other APR components increases the levels of resistance, which suggests that combination of certain APR components should be another important strategy for breeding cultivars conferring durable and adequate levels of resistance. A new adult plant leaf rust resistance source that seems to be associated with durability in ‘Arjun’ has been postulated. Likewise, cultivars possessing Sr2 in combination with certain other specific genes have maintained resistance to stem rust. Further, non-specific resistances that were transferred across widely different genotypes into two of the popular Indian wheats provided easily usable materials to the national breeding programmes for imparting durable resistance to stripe rust.
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  • 91
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    Ecological research 11 (1996), S. 283-289 
    ISSN: 1440-1703
    Keywords: herbivory ; leaf life span ; leaf toughness ; nitrogen ; Quercus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seasonal changes in leaf traits and the herbivory pattern ofQuercus mongolica var.grosseserrata were studied, and simulated herbivory experiments were carried out in order to evaluate leaf trait responses. Leaves ofQ. mongolica emerged simultaneously in spring and most were retained until autumn. Nitrogen concentration was highest when leaves first emerged and decreased rapidly with leaf age. Leaf mass per area (LMA) increased with leaf age. Herbivore attack was concentrated in the first 20 days after bud-break, which corresponded to the high nutritional value of the leaves for herbivores at this time. Simulated herbivory experiments indicated that LMA increased with artificial leaf damage, suggesting an increase in leaf toughness, and that nitrogen concentration decreased later in the season in comparison with intact leaves. As a result, herbivore attack following artificial leaf damage decreased with increasing initial leaf damage. However, leaf longevity was not affected by initial leaf damage. These responses were considered to be a strategy to disperse herbivory damage among leaves.
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  • 92
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    World journal of microbiology and biotechnology 12 (1996), S. 379-384 
    ISSN: 1573-0972
    Keywords: Agaricus bisporus ; ammonia ; compost ; nitrogen ; thermophilic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The rise in temperature is an important event during the composting of lignocellulosic materials and has to be controlled and regulated to improve the biodegradation. Phase I mushroom composts were incubated under environmentally controlled conditions. When the temperature was pre-set at 65°C and then later at around 80°C, the microbial respiration and the biodegradations were hardly affected. However residual activities due to thermoresistant bacteria were still measured after 68 h at a constant temperature of 80°C. Significant changes in nitrogen resources were observed: loss of nitrogen from microbial products, an increase in the proportion of nitrogen in non-hydrolysable and stable forms, and an increase in volatilisation of ammonia. These changes were mainly due to physico-chemical mechanisms associated with disturbances in the structure of the microbial community.
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  • 93
    ISSN: 1573-0867
    Keywords: fertilizer ; nitrogen ; nutrition ; pastures ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to propose consistent decision rules for fertilizer supply, a study was made on the effect of additions of N and P fertilizers and of their interaction on the above-ground dry matter yield of pastures during spring. The interaction between N and P could occur through nutrient acquisition or nutrient efficiency for growth. We therefore characterised the herbage N and P status (N and P index) from previously established critical curves of herbage mineral content according to above-ground dry matter. First we studied the effect of N and P addition on herbage nutrient status. Secondly, we expressed the above-ground dry matter as a function of the herbage nutrient status. This study consisted of four treatments applied to four permanent pastures which had a low phosphorus availability. The results showed a positive effect of P supply on the herbage nitrogen status, which may be due to an increase of organic matter mineralization or root growth. The P herbage status decreased only if N was supplied without P. The dry matter yield was positively related to the herbage nitrogen status, but a low P herbage status reduced the slope of the relationship. For the pastures studied, the indirect effect of P supply on above-ground dry matter, revealed by an increase in N index, was greater than its direct effect. This methodology allows us to distinguish the direct and indirect effects of N and P addition on herbage growth at field level. It could be used to propose consistent rules to manage jointly both N and P supplies.
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  • 94
    ISSN: 1573-0867
    Keywords: China ; Loess Plateau ; maize ; 15N ; Nitrogen ; urea ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field trials were carried out to study the fate of15N-labelled urea applied to summer maize and winter wheat in loess soils in Shaanxi Province, north-west China. In the maize experiment, nitrogen was applied at rates of 0 or 210 kg N ha−1, either as a surface application, mixed uniformly with the top 0.15 m of soil, or placed in holes 0.1 m deep adjacent to each plant and then covered with soil. In the wheat experiment, nitrogen was applied at rates of 0, 75 or 150 kg N ha−1, either to the surface, or incorporated by mixing with the top 0.15 m, or placed in a band at 0.15 m depth. Measurements were made of crop N uptake, residual fertilizer N and soil mineral N. The total above-ground dry matter yield of maize varied between 7.6 and 11.9 t ha−1. The crop recovery of fertilizer N following point placement was 25% of that applied, which was higher than that from the surface application (18%) or incorporation by mixing (18%). The total grain yield of wheat varied between 4.3 and 4.7 t ha−1. In the surface applications, the recovery of fertilizer-derived nitrogen (25%) was considerably lower than that from the mixing treatments and banded placements (33 and 36%). The fertilizer N application rate had a significant effect on grain and total dry matter yield, as well as on total N uptake and grain N contents. The main mechanism for loss of N appeared to be by ammonia volatilization, rather than leaching. High mineral N concentrations remained in the soil at harvest, following both crops, demonstrating a potential for significant reductions in N application rates without associated loss in yield.
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  • 95
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    Nutrient cycling in agroecosystems 47 (1996), S. 197-212 
    ISSN: 1573-0867
    Keywords: ammonia volatilization ; coarse textured soils ; denitrification ; fertilizer N use efficiency ; irrigated soils ; leaching ; nitrification ; nitrogen ; porous soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Porous soils are characterized by high infiltration, low moisture retention and poor fertility due to limitation of organic matter and nitrogen (N). However, wherever irrigated and properly managed, these are among the most productive soils in the world. For sustained productivity and prevention of N related pollution problems, fertilizer N management in porous soils needs to be improved by reducing losses of N via different mechanisms. Losses of N through ammonia volatilization are not favoured in porous soils provided fertilizer N is applied before an irrigation or rainfall event. Ammonium N transported to depth along with percolating water cannot move back to soil surface where it is prone to be lost as NH3. Under upland conditions nitrification proceeds rapidly in porous soils. Due to high water percolation rates in porous soils, continuous flooding for rice production usually cannot be maintained and alternate flood and drained conditions are created. Nitrification proceeds rapidly during drained conditions and nitrates thus produced are subsequently reduced to N2 and N2O through denitrification upon reflooding. Indirect N-budget estimates show that up to 50% of the applied N may be lost via nitrification-denitrification in irrigated porous soils under wetland rice. High soil nitrate N levels and sufficient downward movement of rain water to move nitrate N below the rooting depth are often encountered in soils of humid and subhumid zones, to a lesser extent in soils of semiarid zone and quite infrequently, if at all in arid zone soils. The few investigations carried out with irrigated porous soils do not show substantial leaching losses of N beyond potential rooting zone even under wetland rice. However, inefficient management of irrigation water and fertilizer N particularly with shallow rooted crops may lead to pollution of groundwater due to nitrate leaching. At a number of locations, groundwater beneath irrigated porous soils is showing increased nitrate N concentrations. Efficient management of N for any cropping system in irrigated porous soils can be achieved by plugging losses of N via different mechanisms leading to both high crop production and minimal pollution of the environment.
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    Nutrient cycling in agroecosystems 46 (1996), S. 97-101 
    ISSN: 1573-0867
    Keywords: ammonia loss ; iron pyrite ; nitrogen ; particle size ; placement ; surface-applied urea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Laboratory incubations were conducted to determine the ammonia (NH3) loss from urea as affected by the addition of coarse and ground (fine) pyrites at 1:1, 1:2 and 1:5 urea: pyrite (w/w) ratios and methods of application (surfaceapplication, incorporation and placement). Coarse pyrites (〉-2mm) were not effective in reducing NH3 loss from urea when surface applied even at the highest ratio of pyrite (15.9% vs 18.7% without pyrite). Ground pyrites (0.1–0.25 mm), in 1:1 ratio, had about 5% less NH3 loss than the urea alone treatment. Higher ratios of pyrites reduced NH3 loss much more. Ammonia losses were the most with surface-applied urea (18.9%) and the least (13.5%) when placed (2.5 cm) below the soil surface. Addition of ground pyrite to surface-applied urea (1:1 ratio) decreased the loss to 13.2%. Urea+pyrite placed below the soil surface had the least loss (9.8%). Results indicate that combined application of urea and fine pyrite could reduce NH3 loss.
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  • 97
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    International journal of thermophysics 17 (1996), S. 1349-1363 
    ISSN: 1572-9567
    Keywords: Lennard Jones two-center potential model ; molecular dynamics simulations ; nitrogen ; thermodynamic properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Molecular dynamics simulation results in the isochoric isothermal ensemble are reported for a two-center Lennard Jones model of fluid nitrogen characterized by the fixed molecular elongationL = 1σ = 0.3292, New values ofε andσ were determined by fitting the vapor pressure and the saturated liquid density of the model to experimental data at 94,67 K. The required vapor liquid equilibrium data of the model were taken from a study using the NpT + test particle method. The resulting values areε k = 36.32013 K (36.673 K) andσ = 0.32973 nm (0.33073 nm), with values in parentheses being those obtained previously from a Weeks Chandler Andersen-type perturbation theory. Then pressures and internal energies were calculated by molecular simulations for 110 state points in the temperature range from 72 to 330 K and for densities up to 35 mol · L1. Comparison of the predictions based on the new parameters with the empirical equation of state of Jacobsen et al. shows good to excellent agreement except in the near-critical region. Moreover. for almost all state points the new parameters yield an improvement over old ones from perturbation theory.
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    Plant growth regulation 18 (1996), S. 201-206 
    ISSN: 1573-5087
    Keywords: paclobutrazol ; protection ; stress ; waterlogged ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat (Triticum aestivum L.) seeds were imbibed for 18 h in 40 mM KCl solution containing either distilled water or 100 mg l−1 paclobutrazol (PBZ, a triazole) and then air dried. Seeds were germinated in a greenhouse and one set of seedlings was grown under non-stressed conditions. Another three sets were stressed by waterlogging at three different stages when the PBZ-treated seedlings were 3, 6, or 10 cm above the soil surface. During waterlogging, the soil was saturated by maintaining the water level 3 cm above the soil surface. For waterlogged plants, the first visible symptoms of chlorosis and wilting occurred 2 to 4 days after stress was initiated. There was a concomitant decrease in chlorophyll (a+b) and carotenoid levels. A decrease in fluorescence and increase in leakiness indicated photoinhibitory damage, lower photosynthetic efficiency, and a loss of membrane integrity. These symptoms of stress induced by waterlogging at different stages were reduced by PBZ.
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  • 99
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 47 (1996), S. 27-32 
    ISSN: 1573-5044
    Keywords: growth regulators ; nitrogen ; Pelargonium ; sucrose ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A number of media constituents including sucrose, ammonium nitrate and plant growth regulators were evaluated in an attempt to improve somatic embryo production in zonal geranium (Pelargonium ×hortorum) cv. Scarlet Orbit Improved. Somatic embryo production was characterized by the quantity and type of somatic embryo induced by the treatments. Sucrose at 4% supported the highest number of total somatic embryos while improving the proportion of the morphologically normal cotyledon-stage somatic embryos. Addition of ammonium nitrate also improved embryo production. With 1.89 mM ammonium nitrate, normal cotyledon-stage embryo development was increased by 53%; the proportion of normal cotyledon-stage embryos decreased and abnormal embryos with leaves or serrated margins in cotyledons (fringed-shoot type) increased with higher ammonium nitrate concentrations. The effect of plant growth regulators on somatic embryogenesis indicated that exogenous supply of indole-3-acetic acid (IAA) at a range of 0.25 to 4 µM failed to promote somatic embryogenesis. In contrast, benzyladenine (BA) up to 2.0 µM increased the total embryo number and the proportion of desirable cotyledon-stage embryos. There was no interaction between IAA and BA. Our research has demonstrated that improvement in both quantity and quality of somatic embryos can be achieved in zonal geranium.
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  • 100
    ISSN: 1573-5060
    Keywords: gramineae ; polyembryony ; somatic embryogenesis ; Triticum aestivum ; wheat ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the course of experiments performed to obtain haploid wheat plants in which 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to developing spikes, it was found that three cultivars showed a different ability to produce polyembryos (Thatcher 20.19%, Chris 7.06%, Dollar 0%). This behaviour was related to their capacity to form somatic embryos. Diploid immature embryos cultured in vitro after 2,4-D treatment, gave a higher frequency of embryogenic callus in Thatcher and Chris than in Dollar. As the common factor in both experiments was the 2,4-D treatment we propose that the three cultivars showed a differential sensitivity to 2,4-D.
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