Publication Date:
1989-06-23
Description:
Although the T cell receptor (TCR) alpha beta heterodimer and its encoding genes have been characterized, a cell-free form of this receptor, which is needed for the study of functional or ligand-binding properties of the receptor, has not previously been isolated. When the cell-free supernatant products of activated cloned T helper (TH) cells were found to mediate helper activity with antigen specificity identical to that of intact T cells, experiments were carried out to determine whether this functional activity was mediated by a cell-free form of TCR-related material. A disulfide-linked dimer indistinguishable from the T cell surface alpha beta heterodimer was precipitated from cell-free supernatants of cloned TH cells with F23.1, a monoclonal antibody specific for a TCR V beta 8 determinant. Moreover, when cell-free TH products were bound to and eluted from immobilized F23.1, these affinity-purified materials had antigen-specific and major histocompatibility complex-restricted helper activity that synergized with recombinant lymphokines in the generation of B cell antibody responses. These findings suggest that the factor isolated from T cell supernatants is a cell-free form of the TCR alpha beta dimer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Guy, R -- Ullrich, S J -- Foo-Philips, M -- Hathcock, K S -- Appella, E -- Hodes, R J -- New York, N.Y. -- Science. 1989 Jun 23;244(4911):1477-80.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Experimental Immunology Branch, National Cancer Institute, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2472009" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Monoclonal
;
Antigens/*immunology
;
B-Lymphocytes/immunology
;
Disulfides
;
Epitopes/immunology
;
Hemocyanin/immunology
;
Histocompatibility Antigens/immunology
;
Immunoglobulin G/immunology
;
Immunosorbent Techniques
;
Interleukin-2/pharmacology
;
Interleukin-4
;
Interleukins/pharmacology
;
Lymphocyte Activation
;
Macromolecular Substances
;
Mice
;
Molecular Weight
;
Receptors, Antigen, T-Cell/*immunology/isolation & purification
;
Recombinant Proteins
;
Serum Albumin, Bovine/immunology
;
T-Lymphocytes, Helper-Inducer/*immunology
;
Trinitrobenzenes/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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